CN101356896A - Method for producing Gypsophila seedling using mini-type cuttage - Google Patents
Method for producing Gypsophila seedling using mini-type cuttage Download PDFInfo
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- CN101356896A CN101356896A CNA2008100589449A CN200810058944A CN101356896A CN 101356896 A CN101356896 A CN 101356896A CN A2008100589449 A CNA2008100589449 A CN A2008100589449A CN 200810058944 A CN200810058944 A CN 200810058944A CN 101356896 A CN101356896 A CN 101356896A
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- 241001316290 Gypsophila Species 0.000 title claims abstract description 12
- 238000004519 manufacturing process Methods 0.000 title abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 29
- 229920001817 Agar Polymers 0.000 claims description 16
- 239000008272 agar Substances 0.000 claims description 16
- 238000005286 illumination Methods 0.000 claims description 15
- 238000010899 nucleation Methods 0.000 claims description 14
- 230000035755 proliferation Effects 0.000 claims description 14
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 13
- 229930006000 Sucrose Natural products 0.000 claims description 13
- 239000011159 matrix material Substances 0.000 claims description 13
- 230000001939 inductive effect Effects 0.000 claims description 11
- 241000283986 Lepus Species 0.000 claims description 9
- 230000003203 everyday effect Effects 0.000 claims description 8
- 235000019362 perlite Nutrition 0.000 claims description 7
- 239000010451 perlite Substances 0.000 claims description 7
- 238000005520 cutting process Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 239000012467 final product Substances 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 4
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 4
- 239000006013 carbendazim Substances 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 239000003337 fertilizer Substances 0.000 claims description 4
- 239000011888 foil Substances 0.000 claims description 4
- 229910001710 laterite Inorganic materials 0.000 claims description 4
- 239000011504 laterite Substances 0.000 claims description 4
- 229960002523 mercuric chloride Drugs 0.000 claims description 4
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 4
- 239000002985 plastic film Substances 0.000 claims description 4
- 229920006255 plastic film Polymers 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 239000008223 sterile water Substances 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 239000012879 subculture medium Substances 0.000 claims description 3
- 229910001562 pearlite Inorganic materials 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 12
- 238000005516 engineering process Methods 0.000 abstract description 7
- 230000006698 induction Effects 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 7
- 230000012010 growth Effects 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000008935 nutritious Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 description 1
- 239000005985 Paclobutrazol Substances 0.000 description 1
- 239000005842 Thiophanate-methyl Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
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- Y02P60/216—
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- Cultivation Of Plants (AREA)
Abstract
The invention provides a method for rapidly producing gypsophila seedlings by micro-cuttage. Tradable high-quality seedlings can be obtained by selecting explants, establishing a germfree system, performing subculturing and propagation, induction culture of root primordium, micro-cuttage, managing cottage seedlings, and transplanting the cottage seedlings. The method shortens seedling production time, prolongs seedling supply time and improves seedling quality. Compared with the prior art, the survival rate is increased by 6.2%, the survival rate of the fixed planting is increased by 5.8%, and the high-quality seedlings reaches over 85%, which is an economical, practical and efficient seedling production technology.
Description
Technical field
The present invention relates to a kind of quick production method of cut-flower seedling of open herding, especially a kind of method of producing Gypsophila seedling using mini-type cuttage belongs to plant biotechnology field.
Background technology
Babysbreath is that the most popular fresh cut-flowers in the world is joined one of flower, also is one of Europe ten big cut-flowers.China is mainly in Yunnan Province's plantation, and nearly 4000 mu of cultivated area needs seedling more than 300 ten thousand strains every year.The production of China's babysbreath is mainly based on tissue culture propagation, someone has carried out babysbreath cuttage production test research and has produced, but more in the technical problem of aspects such as illumination, water management, cuttage survival rate and planting percent are not high, and the root system of seedling is relatively poor, and transplanting survival rate is lower.Adopt tissue culture propagation to carry out tissue cultivating seedling production, the foundation of sterile system is easy, reproduction speed is fast, but rootage duration is long in the bottle, needs more than 16 days, and seedling fast growth, the height of seedling reaches more than the 5cm, directly influences the survival rate of transition cultivating, and the phenomenon of " high pin seedling " can occur, can lodge after the plantation, thereby influence the growth of plant and the quality of blooming in later stage.
For the existing article report of the tissue culture of babysbreath, but main research aspect culture medium prescription is more, and mainly be at solving the vitrifying problem and reducing cost and carry out the research of aspects such as hormone combination, paclobutrazol, sugar, fine jade refer to, for at solving the seedling quality problem, and the relevant report that adopts tissue culture and minitype cuttage to combine is not seen.
Summary of the invention
At production problems such as " the high pin seedling " quality problems that exist in existing babysbreath cuttage and the tissue culture and survival rate and planting percent are low, also long and comparatively concentrated for the seedling time simultaneously in order to solve existing seedling, be difficult to produce in a large number in the short period of time serial problems such as seedling, the invention provides a kind of method of producing Gypsophila seedling using mini-type cuttage.
The present invention is to provide a kind of tissue culture technology that adopts earlier and carry out the propagation of seedling and inducing of root original hase, again in conjunction with the minitype cuttage technology, emphasis is to cutting medium, liquid manure and environment manage, to improve the quality of tissue cultivating seedling, make nursery stock short strong, following ground planting survival rates height can anniversary production high-quality tissue cultivating seedling.
The present invention finishes by following technical proposal: a kind of method of producing Gypsophila seedling using mini-type cuttage is characterized in that through the following step:
A, the selection of explant and the foundation of sterile system: obvious in feature, blooming, selecting and purchasing is new on the good stand sprouts and sturdy twig, remove blade, cut and stay the 0.5-1.0cm terminal bud, peel off outer blade, after in adding the water of an amount of washing agent, cleaning, in concentration is sterilization 15 minutes in 0.14% the mercuric chloride solution, be sterilization 10 minutes among 0.2% the liquor natrii hypochloritis in concentration again, after rinsed with sterile water 2 times, be inoculated into proliferated culture medium: among MS+BA1.0～1.5mg/L+NAA0.1mg/L+ agar 6.5～7.0g/L+ white sugar 30～40g/L, in temperature is 24-26 ℃, and intensity of illumination is 2000～2500Lx, and light application time is under 10-12 hour/day the condition, enrichment culture 10～20 days promptly gets Cong Zhuanmiao;
B, shoot proliferation are cultivated: the band joint segment that the clump shape seedling of A step acquisition is cut into a 1.5cm length and a tool 1-3 bud, be inoculated into the shoot proliferation medium: among MS+BA1.0～1.5mg/L+NAA0.1mg/L+ agar 6.5～7.0g/L+ white sugar 30～40g/L, in temperature is 24-26 ℃, intensity of illumination is 2000～2500Lx, light application time is under 10-12 hour/day the condition, successive transfer culture 12～15 days gets the shoot proliferation seedling, and subculture cycle is 12-15 days.
C, root original hase inducing culture: the seedling that above-mentioned shoot proliferation seedling is cut into band stem apex 1-1.2cm, be inoculated into inducing culture: MS+NAA0.2～0.3mg/L+IAA0.1～0.2mg/L+ agar 6.5～7.0g/L+ white sugar 30～40g/L, on the pH value 5.8～6.0, and it is 24-26 ℃ in cultivation temperature, intensity of illumination is 2000～2500Lx, light application time is under 8-10 hour/day the condition, inducing culture 6-7 days, forms the root original hase-seedling of promptly taking root;
D, minitype cuttage: the above-mentioned seedling of taking root is taken out the back in concentration is 0.1% carbendazim solution, soaked 1-2 minute, after after the taking-up seedling base portion being stained with the root sun solution of 100ppm, cuttage is in pearlite interstitial substance, and stromal thickness is 2.5～3.0cm, and cutting density is 1.0cm * 1.5cm;
The management of E, cuttage seeding: after the seedling cuttage, spray normal root water, afterwards that cuttage is good seedling amplifies in the canopy and covered with plastic film, and add a cover two-layer 70% sunshade net again, keep air humidity more than 95%, remove plastic foil after 3 days, air humidity is controlled at more than 90%, removed one deck sunshade net in 3-4 days, air humidity remains on more than 80%, after 1 week the sunshade net is all removed, but need lid layer sunshade net again when sunlight is strong at noon, water 2-3 every day, only needs to water every morning after 10 days once to get final product, and transplants after 20 days～3 months;
The transplanting of F, cuttage seeding: above-mentioned cuttage seeding is taken out from matrix, being transplanted to mass ratio is laterite: leaf mould: in the matrix of perlite=1.5: 1.0: 0.5, after watering normal root water, sunshade cultivation off the net 3-5 days, carry out normal management afterwards, water 1-2 every day, sprayed once-combined fertilizer in the time of 7-10 days, transplanted 16-20 days, and promptly became the high quality seedling that can go on the market.
The present invention compared with prior art has following advantage and effect,
1. improved transplanting survival rate and seedling quality: prior art be in bottle, take root and the long nearly 1cm of root after just emerge, root system is easily impaired during transplanting, the seedling frangibility, and the difficult wash clean of the agar on the root system, easily introduce mashed seedling, survival rate is lower, and transplanting survival rate of the present invention reaches 98.2%.
2. effectively solved " high pin seedling " problem of seedling of open herding, seedling quality obviously improves: after adopting the minitype cuttage technology, took root 6-8 days in the bottle, this moment, the root original hase formed, and the growth of seedling just just begins, carry out root system ramp behind the minitype cuttage again, the quick growth of seedling is eased, simultaneously under the stronger illumination condition in field, and the robust growth of seedling, survival rate improves 5.8% after the field planting, and growth rapidly.
3. the minitype cuttage technology has been simplified the production details, save the labour, improved operating efficiency: prior art is because of forming root system in the bottle, the agar of wanting flush away to stick on the root system when emerging is to take a lot of work most in the whole transplanting, insert and bottle outlet is little when only forming the root original hase, then base portion can not glue agar, directly takes out the immersion bactericide and just can directly carry out minitype cuttage.
Embodiment
Below in conjunction with embodiment the present invention is described further.
Embodiment 1
1, the selection of explant and the foundation of sterile system: obvious when selecting explant from varietal characteristic, bloom and show new sprouting of selecting and purchasing and sturdy vegetative bud on the good plant, remove blade, cut and stay the 0.5cm terminal bud, peel off outer blade, after in the water that adds the clear agent of a small amount of washing, cleaning, sterilization is after 15 minutes in 0.14% mercuric chloride solution, in 0.2% liquor natrii hypochloritis, sterilized 10 minutes again, be inoculated into proliferated culture medium after the rinsed with sterile water 2 times: cultivate in the medium of MS+BA1.0mg/L+NAA0.1mg/L+ agar 6.5g/L+ white sugar 30g/L, in temperature is 24 ℃, intensity of illumination is 2000Lx, light application time is that enrichment culture 20 days promptly gets Cong Zhuanmiao under 12 hours/day the condition;
2, shoot proliferation is cultivated: the band joint segment that the clump shape seedling of A step acquisition is cut into a 1.5cm length and a tool 1-3 bud, be transferred on the subculture medium of MS+BA1.0mg/L+NAA0.1mg/L+ agar 6.5g/L+ white sugar 30g/L, in temperature is 24 ℃, intensity of illumination is 2000Lx, light application time is under 10 hours/day the condition, successive transfer culture 15 days gets the shoot proliferation seedling, and every 12-15 days subculture once during subculture;
3, root original hase inducing culture: with the shoot proliferation seedling, be cut into the seedling of band stem apex 1-1.2cm, be inoculated into inducing culture: MS+NAA0.2mg/L+IAA0.1mg/L+ agar 6.5g/L+ white sugar 30g/L, on the medium of pH value 5.8, and be 24 ℃ in cultivation temperature, intensity of illumination is 2000Lx, light application time is under 10 hours/day the condition, cultivated 7 days, and promptly formed the root original hase-seedling of taking root;
4, minitype cuttage: the above-mentioned seedling of taking root is taken out the back in concentration is 0.1% carbendazim solution, soaked 1 minute, after after the taking-up seedling base portion being stained with the root sun solution of 100ppm, cuttage is to using in the seedling-cultivating tray of perlite as matrix, and stromal thickness is 2.5cm, and cutting density is 1.0 * 1.5cm;
5, the management of cuttage seeding: after the seedling cuttage is finished, spray normal root water immediately, afterwards will be seedling that cuttage is good amplify in the canopy and covered with plastic film, and add a cover two-layer 70% sunshade net again, keep air humidity more than 95% by spraying, remove plastic foil after 3 days, air humidity was removed one deck sunshade net more than 90% after 3 days, air humidity remains on more than 80%, after 1 week the sunshade net is all removed, but need cover a room sunshade net again when sunlight is strong at noon.Water every day 2 times, only need to water every morning after 10 days once to get final product, can transplant after 20 days, the characteristics of the method are that the seedling in the pearl rock can be by the control of moisture and nutrient, transplant the quality that still can guarantee the product seedling in 3 months;
6, the transplanting of cuttage seeding: above-mentioned 20 days cuttage seeding is taken out from matrix, be transplanted in the nutritious bag that following mixed-matrix is housed, the mass ratio of mixed-matrix is: laterite: leaf mould: perlite=1.5: 1.0: 0.5, after watering normal root water, lid sunshade net carries out normal management after 3 days, water 1-2 every day, sprayed once-combined fertilizer in the time of 7 days, transplanted 16 days, and promptly became the high quality seedling that can go on the market.
Embodiment 2
1, the selection of explant and the foundation of sterile system: obvious when selecting explant from varietal characteristic, bloom and show new sprouting of selecting and purchasing and sturdy vegetative bud on the good plant, remove blade, cut and stay the 1.0cm terminal bud, peel off outer blade, after in the water that adds the clear agent of a small amount of washing, cleaning, sterilization is after 15 minutes in 0.14% mercuric chloride solution, in 0.2% liquor natrii hypochloritis, sterilized 10 minutes again, be inoculated into proliferated culture medium after the rinsed with sterile water 2 times: cultivating in the medium of MS+BA1.5mg/L+NAA0.1mg/L+ agar 7.0g/L+ white sugar 40g/L, is 26 ℃ in temperature, and intensity of illumination is 2500Lx, light application time is under 10 hours/day the condition, to cultivate 10 days;
2, shoot proliferation is cultivated: the band joint segment that the clump shape seedling of A step acquisition is cut into a 1.5cm length and a tool 1-3 bud, be transferred on the subculture medium of MS+BA1.5mg/L+NAA0.1mg/L+ agar 7.0g/L+ white sugar 40g/L, in temperature is 26 ℃, intensity of illumination is 2500Lx, light application time is under 10 hours/day the condition, successive transfer culture 12 days gets the shoot proliferation seedling, and every 12-15 days subculture once during subculture;
3, root original hase inducing culture: with the shoot proliferation seedling, be cut into the seedling of band stem apex 1.2cm, be inoculated into MS+NAA0.3mg/L+IAA0.1mg/L+ agar 7.0g/L+ white sugar 40g/L, on the medium of pH value 6.0, and be 26 ℃ in cultivation temperature, intensity of illumination is 2500Lx, light application time is under 8 hours/day the condition, cultivated 6 days, and promptly formed the root original hase-seedling of taking root;
4, minitype cuttage: the above-mentioned seedling of taking root is taken out the back in concentration is 0.1% carbendazim solution, soaked 2 minutes, after after the taking-up seedling base portion being stained with the root sun solution of 100ppm, cuttage is to using in the seedling-cultivating tray of perlite as matrix, and stromal thickness is 2.5cm, and cutting density is 1.0 * 1.5cm;
5, the management of cuttage seeding: after the seedling cuttage is finished, spray normal root water immediately, afterwards will be seedling that cuttage is good amplify in the canopy and covered with plastic film, and add a cover two-layer 70% sunshade net again, keep air humidity more than 95% by spraying, remove plastic foil after 3 days, air humidity was removed one deck sunshade net more than 90% after 4 days, air humidity remains on more than 80%, after 1 week the sunshade net is all removed, but need cover a room sunshade net again when sunlight is strong at noon.Water 2-3 every day, only needs to water every morning after 10 days once to get final product, and can transplant after 20 days, and the characteristics of the method are that the seedling in the pearl rock can be by the control of moisture and nutrient, transplant the quality that still can guarantee the product seedling in 3-4 month;
6, the transplanting of cuttage seeding: above-mentioned 3 months cuttage seeding is taken out from matrix, in the nutritious bag that mixed-matrix is housed of being transplanted to, the quality proportioning of mixed-matrix is: laterite: leaf mould: perlite=1.5: 1.0: 0.5, after watering normal root water, lid sunshade net carries out normal management after 3-5 days, water 1-2 every day, sprayed once-combined fertilizer in the time of 10 days, transplanted 20 days, and promptly became the high quality seedling that can go on the market.
Subordinate list one: minitype cuttage technology and existing transplanting, planting survival rates and seedling quality contrast table
Embodiment 3
The foundation of explant selection and sterile system is with embodiment 1.
Inducing of root original hase: with the shoot proliferation seedling, be cut into the seedling of band stem apex 1cm, be inoculated into MS+IBA0.3mg/L+ agar 7.0g/L+ white sugar 30g/L, on the medium of pH value 6.0, and be 26 ℃ in cultivation temperature, intensity of illumination is 2500Lx, light application time is under 8 hours/day the condition, cultivated 6-7 days, and behind the formation root original hase, carried out minitype cuttage.
Minitype cuttage: the above-mentioned seedling of taking root is taken out the back in 0.15% thiophanate methyl solution, soaked 1 minute from bottle, cuttage is in the seedling-cultivating tray of perlite as matrix after after the taking-up seedling base portion being stained with the root sun solution of 100ppm, stromal thickness is 2.5～3.0cm, and cutting density is 1.0cm * 1.5cm.
The management of cuttage seeding: with embodiment 1.
The transplanting of cuttage seeding: with embodiment 1.
Claims (1)
1, a kind of method of producing Gypsophila seedling using mini-type cuttage is characterized in that through the following step:
A, the selection of explant and the foundation of sterile system: obvious in feature, blooming, selecting and purchasing is new on the good stand sprouts and sturdy twig, remove blade, cut and stay the 0.5-1.0cm terminal bud, peel off outer blade, after in adding the water of an amount of washing agent, cleaning, in concentration is sterilization 15 minutes in 0.14% the mercuric chloride solution, be sterilization 10 minutes among 0.2% the liquor natrii hypochloritis in concentration again, after rinsed with sterile water 2 times, be inoculated into proliferated culture medium: among MS+BA1.0～1.5mg/L+NAA0.1mg/L+ agar 6.5～7.0g/L+ white sugar 30g/L, in temperature is 24-26 ℃, and intensity of illumination is 2000～2500Lx, and light application time is under 10-12 hour/day the condition, enrichment culture 15～20 days, get final product Cong Zhuanmiao;
B, shoot proliferation are cultivated: the band joint segment that the clump shape seedling of A step acquisition is cut into a 1.5cm length and a tool 1-3 bud, be inoculated into subculture medium: among MS+BA1.0mg/L+NAA0.1mg/L+ agar 6.5～7.0g/L+ white sugar 30g/L, in temperature is 24-26 ℃, intensity of illumination is 2000～2500Lx, light application time is under 10-12 hour/day the condition, successive transfer culture 15～20 days gets the shoot proliferation seedling, and subculture cycle is 12-15 days;
C, root original hase inducing culture: with above-mentioned shoot proliferation seedling, be cut into the seedling of band stem apex 1-1.2cm, be inoculated into inducing culture: MS+NAA0.2～0.3mg/L+IAA0.1～0.2mg/L+ agar 6.5～7.0g/L+ white sugar 30g/L, on the pH value 5.8～6.0, and be 24-26 ℃ in cultivation temperature, intensity of illumination is 2000～2500Lx, light application time is under 8-10 hour/day the condition, inducing culture 6-7 days, form the root original hase-seedling of promptly taking root;
D, minitype cuttage: the above-mentioned seedling of taking root is taken out the back in concentration is 0.1% carbendazim solution, soaked 1-2 minute, after after the taking-up seedling base portion being stained with the root sun solution of 100ppm, cuttage is in pearlite interstitial substance, and stromal thickness is 2.5～3.0cm, and cutting density is 1.0cm * 1.5cm;
The management of E, cuttage seeding: after the seedling cuttage, spray normal root water, afterwards that cuttage is good seedling amplifies in the canopy and covered with plastic film, and add a cover two-layer 70% sunshade net again, keep air humidity more than 95%, remove plastic foil after 3 days, air humidity is controlled at more than 90%, removed one deck sunshade net in 3-4 days, air humidity remains on more than 80%, after 1 week the sunshade net is all removed, but need lid layer sunshade net again when sunlight is strong at noon, water 2-3 every day, only needs to water every morning after 10 days once to get final product, and transplants after 20 days～3 months;
The transplanting of F, cuttage seeding: above-mentioned cuttage seeding is taken out from matrix, being transplanted to mass ratio is laterite: leaf mould: in the matrix of perlite=1.5: 1.0: 0.5, after watering normal root water, sunshade cultivation off the net 3-5 days, carry out normal management afterwards, water 1-2 every day, sprayed once-combined fertilizer in the time of 7-10 days, transplanted 16-20 days, and promptly became the high quality seedling that can go on the market.
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| CN101946707A (en) * | 2010-08-25 | 2011-01-19 | 云南省农业科学院花卉研究所 | Tissue culture and rapid propagation method of million stars |
| CN102318485A (en) * | 2011-07-27 | 2012-01-18 | 金华职业技术学院 | Cuttage propagation method based on nutrient solution spraying |
| CN105961015A (en) * | 2016-06-15 | 2016-09-28 | 安徽菲扬农业科技有限公司 | Gypsophila paniculata planting technology |
| CN105993558A (en) * | 2016-06-27 | 2016-10-12 | 安徽梅兰园林景观工程有限公司 | Cutting propagation technology for Gypsophila paniculata |
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2008
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| CN105993558A (en) * | 2016-06-27 | 2016-10-12 | 安徽梅兰园林景观工程有限公司 | Cutting propagation technology for Gypsophila paniculata |
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| CN109197545A (en) * | 2018-11-07 | 2019-01-15 | 常宁市瑶园生态农业科技发展有限公司 | A kind of tea cutting mating system |
| CN109463146A (en) * | 2018-12-27 | 2019-03-15 | 靖西市秀美边城农业科技有限公司 | A method of promoting babysbreath seedling cuttage Rapid Rooting |
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