CN108912240A - The extracting method and its application of the high coriolan composition of sugar content - Google Patents
The extracting method and its application of the high coriolan composition of sugar content Download PDFInfo
- Publication number
- CN108912240A CN108912240A CN201811083756.1A CN201811083756A CN108912240A CN 108912240 A CN108912240 A CN 108912240A CN 201811083756 A CN201811083756 A CN 201811083756A CN 108912240 A CN108912240 A CN 108912240A
- Authority
- CN
- China
- Prior art keywords
- coriolan
- composition
- filter residue
- ethyl alcohol
- added
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Materials Engineering (AREA)
- Polymers & Plastics (AREA)
- Biochemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Sustainable Development (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Epidemiology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of extracting methods of coriolan composition that sugar content is high, include the following steps：By rainbow conk and dry ice co-grinding, successively use sodium-chloride water solution, pretreatment liquid processing, filter to take filter residue, ethanol water refluxing extraction is added into filter residue, filtering, take filter residue, distilled water refluxing extraction is added into filter residue, filter to take filtrate, filtrate is concentrated, ethyl alcohol is added, centrifuging and taking precipitating, precipitating is successively used into ethyl alcohol, acetone washing, precipitating after washing is dissolved with distilled water, cross hollow-fibre membrane, collect concentrate, centrifugation, filter to take filtrate, filtrate is spray-dried, up to coriolan composition, with the content for improving coriolan in coriolan composition.The invention also discloses a kind of application of coriolan composition in preparation treatment stomatocace medicine, and there is the drug for preparing treatment canker sore for coriolan composition to provide the beneficial effect on experimental data basis.
Description
Technical field
The present invention relates to coriolan fields.It is more particularly related to a kind of coriolan group that sugar content is high
Close the extracting method and its application of object.
Background technique
Rainbow conk is the Chinese herbal medicine resource of China's preciousness, and prepared from coriolus versicolor mycelium is interior and can produce coriolan, rainbow conk in vitro
Polysaccharide is the main active of rainbow conk, it is containing β-glycosidic bond glucan, and Coriolus Versicolor P.E. dries resolving sputum effect, anti-
The function of bacterium, mould and virus can treat tuberculosis and chronic bronchitis, unresolved or chronic hepatitis, be alternatively arranged as liver cancer
The immunotherapy medicaments of patient.The method that coriolan extracts at present is broadly divided into water extract method, acid by the difference of Extraction solvent
Formulation, alkaline extraction etc., sour formulation and alkaline extraction extract the serious ground contamination environment of meeting of coriolan, and flooding rule is producing
Technique, production cost, Product Safety and environmental protection etc. have stronger advantage.Current water extract method often first uses ethyl alcohol
Coriolan is extracted using the method that rainbow conk is boiled in water heating afterwards, lacks the pre-processing to rainbow conk, the coriolan extracted
The sugar content of composition is relatively low.
Summary of the invention
It is an object of the invention to solve at least the above problems, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of extracting method of coriolan composition that sugar content is high and its answer
With, improve the sugar content in coriolan composition, the present invention also provides a kind of coriolan composition preparation treat
Application in the drug of canker sore, the drug for preparing treatment canker sore for coriolan composition provide experimental data base
Plinth, to increase the types of drugs for the treatment of canker sore.
In order to realize these purposes and other advantages according to the present invention, a kind of coriolan group that sugar content is high is provided
The extracting method for closing object, includes the following steps：
Step 1: by rainbow conk and dry ice by weight 1.5~2.0:1 mixing after, be crushed to 120 mesh with pulverizer hereinafter,
Rainbow conk powder is obtained, the sodium-chloride water solution that rainbow conk powder mass fraction is 0.65~0.85% is impregnated into 30~40min, in 65~75
Heated sealed handles 5~10min at DEG C, filters to take filter residue I, and pretreatment liquid is added into filter residue I and impregnates 2~4h, in 8~
20~30min of slightly boiled processing, filters to take filter residue II under the pressure of 10MPa, and the matter of 5~12 times of volumes is added into filter residue II every time
Ethanol water 2~3h of refluxing extraction that score is 75~85% is measured, refluxing extraction 2~3 times altogether, filtering takes filter residue III,
In, by weight, pretreatment liquid includes：0.2~0.4 part of ursolic acid, 0.1~0.2 part of hesperidin methyl, 0.4~0.6 part hundred
Autumn Lee's alcohol, 4~6 parts of guanidinium isothiocyanates, 1~2 part of lauryl sodium sulfate, 0.5~1 part of sodium citrate, 0.5~1.5 portion of peppermint
Oil, 0.6~0.8 part of gaultherolin, 0.8~1 part of Monoolein, 1~1.5 part of dodecyl trimethyl ammonium chloride, 2~
3 parts of chloroforms, 5~8 parts of isoamyl alcohol, 10~15 parts of ethyl alcohol；
Step 2: distilled water 2~3h of refluxing extraction of 8~12 times of volumes is added into filter residue III every time, total refluxing extraction 3
~4 times, filter to take filtrate；
Step 3: the filtrate in step 2 is concentrated into the medicinal extract that relative density is 1.1~1.3 at 60 DEG C, ethyl alcohol is added,
Centrifuging and taking precipitating；
Step 4: precipitating is successively used ethyl alcohol, acetone washing, 2~4 times of distilled water of the precipitating after washing are dissolved, mistake
Molecular cut off be 1,000,000 hollow-fibre membrane, collect concentrate I, by concentrate I cross molecular cut off be 1,500,000 it is hollow
Tunica fibrosa collects concentrate II, is centrifuged, and filtering takes filtrate；
Step 5: being spray-dried the filtrate in step 4 to get coriolan composition.
Preferably, the concrete operations of addition ethyl alcohol are in step 3：The volume that ethyl alcohol to ethyl alcohol are added into medicinal extract contains
Amount is 80~85%.
Preferably, the revolving speed being centrifuged in step 3 is 2500~3000r/min.
Preferably, the time being spray-dried in step 5 is 8~12h.
Preferably, the weight of ethyl alcohol is 4~6 times precipitated in step 4, and the weight of acetone is 3~6 times of precipitating.
The coriolan composition that the extracting method of the high coriolan composition of the sugar content obtains is controlled in preparation
Treat the application in stomatocace medicine.
The present invention is include at least the following beneficial effects：By rainbow conk and dry ice by weight 1.5~2.0:It is crushed to after 1 mixing
120 mesh hereinafter, rainbow conk powder, by rainbow conk powder mass fraction be 0.65~0.85% sodium-chloride water solution impregnate 30~
40min, heated sealed handles 5~10min at 65~75 DEG C, filters to take filter residue I, and pretreatment liquid is added into filter residue I and impregnates 2
~4h, 20~30min of slightly boiled processing under the pressure of 8~10MPa, aforesaid operations step and pretreatment liquid immersed with being conducive to
Improve the content of coriolan in coriolan composition, coriolan composition Dichlorodiphenyl Acetate that the present invention is extracted from rainbow conk,
The cavy canker sore that staphylococcus epidermis, Candida albicans induce has apparent therapeutic effect, and has dose dependent, cloud
Sesame polysaccharide composition mentions the drug that the drug action of cavy canker sore is coriolan composition preparation treatment canker sore
Supply experimental data basic.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
It should be noted that experimental method described in following embodiments is unless otherwise specified conventional method, institute
Reagent and material are stated, unless otherwise specified, is commercially obtained.
<Embodiment 1>
The extracting method of the high coriolan composition of sugar content, includes the following steps：
Step 1: by rainbow conk and dry ice by weight 1.5:After 1 mixing, 120 mesh are crushed to pulverizer hereinafter, obtaining rainbow conk
The sodium-chloride water solution that rainbow conk powder mass fraction is 0.65% is impregnated 30min by powder, and heated sealed is handled at 65 DEG C
5min filters to take filter residue I, and pretreatment liquid is added into filter residue I and impregnates 2h, the slightly boiled processing 20min under the pressure of 8MPa, filtering
Filter residue II is taken, the ethanol water refluxing extraction 2h that the mass fraction of 5 times of volumes is 75% is added into filter residue II every time, returns altogether
Stream extracts 2 times, and filtering takes filter residue III, wherein by weight, pretreatment liquid includes：0.2 part of ursolic acid, 0.1 part of methyl orange
Skin glycosides, 0.4 part of patchouli alcohol, 4 parts of guanidinium isothiocyanates, 1 part of lauryl sodium sulfate, 0.5 part of sodium citrate, 0.5 portion of peppermint oil,
0.6 part of gaultherolin, 0.8 part of Monoolein, 1 part of dodecyl trimethyl ammonium chloride, 2 parts of chloroforms, 5 parts of isoamyl alcohol,
10 parts of ethyl alcohol；
Step 2: the distilled water refluxing extraction 2h of 8 times of volumes is added into filter residue III every time, refluxing extraction 3 times, is filtered altogether
Take filtrate；
Step 3: the filtrate in step 2 is concentrated into the medicinal extract that relative density is 1.1 at 60 DEG C, second is added into medicinal extract
The volume content of alcohol to ethyl alcohol is 80%, and centrifuging and taking precipitating, the revolving speed of centrifugation is 2500r/min；
Step 4: precipitating is successively used ethyl alcohol, acetone washing, the weight of ethyl alcohol is 4 times of precipitating, and the weight of acetone is heavy
3 times to form sediment, 2 times of distilled water of the precipitating after washing are dissolved, and cross the hollow-fibre membrane that molecular cut off is 1,000,000, are collected dense
Contracting liquid I, crosses the hollow-fibre membrane that molecular cut off is 1,500,000 for concentrate I, collects concentrate II, be centrifuged, and filtering takes filtrate；
Step 5: the filtrate in step 4 is spray-dried, the dry time is 8h to get coriolan combination
Object.
<Embodiment 2>
The extracting method of the high coriolan composition of sugar content, includes the following steps：
Step 1: by rainbow conk and dry ice by weight 2.0:After 1 mixing, 120 mesh are crushed to pulverizer hereinafter, obtaining rainbow conk
The sodium-chloride water solution that rainbow conk powder mass fraction is 0.85% is impregnated 40min by powder, and heated sealed is handled at 75 DEG C
10min filters to take filter residue I, and pretreatment liquid is added into filter residue I and impregnates 4h, slightly boiled processing 30min, mistake under the pressure of 10MPa
The ethanol water refluxing extraction 3h that the mass fraction of 12 times of volumes is 85% is added in leaching filter residue II into filter residue II every time,
Refluxing extraction 3 times altogether, filtering, takes filter residue III, wherein by weight, pretreatment liquid includes：0.4 part of ursolic acid, 0.2 part of first
Base aurantiamarin, 0.6 part of patchouli alcohol, 6 parts of guanidinium isothiocyanates, 2 parts of lauryl sodium sulfate, 1 part of sodium citrate, 1.5 portions of peppermints
Oil, 0.8 part of gaultherolin, 1 part of Monoolein, 1.5 parts of dodecyl trimethyl ammonium chloride, 3 parts of chloroforms, 8 parts of isoamyls
Alcohol, 15 parts of ethyl alcohol；
Step 2: the distilled water refluxing extraction 3h of 12 times of volumes is added into filter residue III every time, and total refluxing extraction 4 times, mistake
Leaching filtrate；
Step 3: the filtrate in step 2 is concentrated into the medicinal extract that relative density is 1.3 at 60 DEG C, second is added into medicinal extract
The volume content of alcohol to ethyl alcohol is 85%, and centrifuging and taking precipitating, the revolving speed of centrifugation is 3000r/min；
Step 4: precipitating is successively used ethyl alcohol, acetone washing, the weight of ethyl alcohol is 6 times of precipitating, and the weight of acetone is heavy
6 times to form sediment, 4 times of distilled water of the precipitating after washing are dissolved, and cross the hollow-fibre membrane that molecular cut off is 1,000,000, are collected dense
Contracting liquid I, crosses the hollow-fibre membrane that molecular cut off is 1,500,000 for concentrate I, collects concentrate II, be centrifuged, and filtering takes filtrate；
Step 5: the filtrate in step 4 is spray-dried, the dry time is 12h to get coriolan combination
Object.
<Embodiment 3>
The extracting method of the high coriolan composition of sugar content, includes the following steps：
Step 1: by rainbow conk and dry ice by weight 1.8:After 1 mixing, 120 mesh are crushed to pulverizer hereinafter, obtaining rainbow conk
The sodium-chloride water solution that rainbow conk powder mass fraction is 0.75% is impregnated 35min by powder, and heated sealed is handled at 70 DEG C
8min filters to take filter residue I, and pretreatment liquid is added into filter residue I and impregnates 3h, the slightly boiled processing 25min under the pressure of 9MPa, filtering
Filter residue II is taken, the ethanol water refluxing extraction that the mass fraction that 8 times of volumes are added into filter residue II every time is 80% flows back
2.5h, refluxing extraction 3 times, filtering take filter residue III, wherein by weight, pretreatment liquid includes altogether：0.3 part of ursolic acid,
0.15 part of hesperidin methyl, 0.5 part of patchouli alcohol, 5 parts of guanidinium isothiocyanates, 1.5 parts of lauryl sodium sulfate, 0.8 part of citric acid
Sodium, 1.0 portions of peppermint oils, 0.7 part of gaultherolin, 0.9 part of Monoolein, 1.2 parts of dodecyl trimethyl ammonium chloride,
2.5 parts of chloroforms, 6 parts of isoamyl alcohol, 13 parts of ethyl alcohol；
Step 2: the distilled water refluxing extraction 2.5h of 10 times of volumes is added into filter residue III every time, total refluxing extraction 3 times,
Filter to take filtrate；
Step 3: the filtrate in step 2 is concentrated into the medicinal extract that relative density is 1.2 at 60 DEG C, second is added into medicinal extract
The volume content of alcohol to ethyl alcohol is 82%, and centrifuging and taking precipitating, the revolving speed of centrifugation is 2800r/min；
Step 4: precipitating is successively used ethyl alcohol, acetone washing, the weight of ethyl alcohol is 5 times of precipitating, and the weight of acetone is heavy
4 times to form sediment, 3 times of distilled water of the precipitating after washing are dissolved, and cross the hollow-fibre membrane that molecular cut off is 1,000,000, are collected dense
Contracting liquid I, crosses the hollow-fibre membrane that molecular cut off is 1,500,000 for concentrate I, collects concentrate II, be centrifuged, and filtering takes filtrate；
Step 5: the filtrate in step 4 is spray-dried, the dry time is 10h to get coriolan combination
Object.
<Comparative example 1>
The extracting method of coriolan composition, includes the following steps：
Step 1: rainbow conk is crushed, the ethanol water refluxing extraction for being every time 80% with the mass fraction of 8 times of volumes
2.5h, refluxing extraction 3 times, filtering take filter residue altogether；
Step 2: the distilled water that 10 times of volumes are added into filter residue every time carries out refluxing extraction 2.5h, total refluxing extraction 3
Secondary, filtering takes filtrate；
Step 3: the filtrate in step 2 is concentrated into the medicinal extract that relative density is 1.2 at 60 DEG C, second is added into medicinal extract
The volume content of alcohol to ethyl alcohol is 82%, and the revolving speed of centrifugation is that 2800r/min takes precipitating；
Step 4: precipitating is successively used ethyl alcohol, acetone washing, the weight of ethyl alcohol is 5 times of precipitating, and the weight of acetone is heavy
4 times to form sediment, 3 times of distilled water of the precipitating after washing are dissolved, and cross the hollow-fibre membrane that molecular cut off is 1,000,000, are collected dense
Contracting liquid I, crosses the hollow-fibre membrane that molecular cut off is 1,500,000 for concentrate I, collects concentrate II, be centrifuged, and filtering takes filtrate；
Step 5: the filtrate in step 4 is spray-dried, drying time is 10h to get coriolan composition.
<Rainbow conk measurement of the polysaccharide content in coriolan composition>
The coriolan composition extracted using the embodiment of the present invention 1~3, comparative example 1 is as evaluation object, using phenol sulphur
Acid system is measured the content of coriolan in coriolan composition, and data are as shown in the table.
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | |
| Coriolan content (%) | 62.5 | 63.4 | 63.0 | 50.2 |
The coriolan content data of Examples 1 to 3 and comparative example 1 the results show that Examples 1 to 3 coriolan group
It closes rainbow conk polyoses content in object and is apparently higher than comparative example 1, show rainbow conk and dry ice by weight 1.5~2.0:Powder after 1 mixing
120 mesh or less are broken into rainbow conk powder, the sodium-chloride water solution that rainbow conk powder mass fraction is 0.65~0.85% is impregnated 30~
40min, heated sealed handles 5~10min at 65~75 DEG C, filters to take filter residue I, and pretreatment liquid is added into filter residue I and impregnates 2
~4h, 20~30min of slightly boiled processing under the pressure of 8~10MPa, aforesaid operations step and pretreatment liquid immersed with being conducive to
The content for improving coriolan in coriolan composition, to improve the purity of coriolan composition.
<The effect experiment of coriolan composition treatment canker sore>
Drug：The extracted coriolan composition of embodiment 3
Experimental animal：Cavy, 300~500g of weight, half male and half female
Bacterial strain：Staphylococcus epidermis, Candida albicans
Experiment one, acetic acid induce canker sore experiment
Cavy 50 for choosing health, the acetic acid that cheek submucosal injection mass fraction is 10% on the left of cavy oral cavity
0.05mL/ only, for 24 hours after can form ulcer, with divider measure ulcer diameter, be randomly divided into 5 groups, i.e. blank by ulcer treatment method
Control group, low dose group, middle dose group, high dose group, positive drug control group, every group 10, low dose group, middle dose group, height
It is respectively 1mg, 2mg, 4mg that dosage group uses the extracted coriolan composition of embodiment 3 every time, and blank control group is not given
Coating substance, positive controls coat Bingpeng San, cavy are placed on fixed frame, atrium is earned with self-control opener and pushes down tongue
Drug is applied on ulcer surface by head, and administration guarantees that drug stops 15~20min, the morning, each coating 1 in afternoon in oral cavity every time
Secondary, continuous 7d before measurement record groups of animals administration, the 4th, 6,8 day ulcer area size after medicine, observes and records each group daily
Ulcer healing number of animals.Compared with blank control group, with the significance of difference between t value method check groups.
1 coriolan composition Dichlorodiphenyl Acetate of table induce cavy canker sore therapeutic effect (N=10)
Experiment two, staphylococcus epidermis induce the experiment of cavy canker sore
Cavy 50, half male and half female, the cheek submucosal injection 15 × 10 on the left of cavy oral cavity8The epidermis grape of CFU/mL
Bacterium bacterium solution 0.07mL/ only, for 24 hours after in booster shots equivalent bacterium solution 1 time again of former injection site, be randomly divided into 5 by ulcer treatment method
Group, i.e. blank control group, low dose group, middle dose group, high dose group, positive drug control group, every group 10, low dose group, in
It is respectively 1mg, 2mg, 4mg, blank pair that dosage group, high dose group use the extracted coriolan composition of embodiment 3 every time
Coating substance is not given according to group, positive controls coat Bingpeng San, cavy is placed on fixed frame, earns opening with self-control opener
Chamber simultaneously pushes down tongue, and drug is applied on ulcer surface, every time administration guarantee drug oral cavity stop 15~20min, the morning, under
Noon each coating 1 time, continuous 7d before measurement record groups of animals administration, the 2nd, 4,6 day ulcer area size after medicine, is seen daily
Examine the ulcer healing number of animals of record each group.Compared with blank control group, with the significance of difference between t value method check groups.
2 coriolan composition of table to staphylococcus epidermis induce cavy canker sore therapeutic effect (N=10)
Experiment three, Candida albicans induce the experiment of cavy canker sore
Cavy 50, half male and half female, the cheek submucosal injection 15 × 10 on the left of cavy oral cavity8The Candida albicans of CFU/mL
Only, i.e. voluntarily ulceration forms ulcer to injection site formation warts to bacterium bacterium solution 0.07mL/ after 48h.5 are randomly divided by ulcer treatment method
Group, i.e. blank control group, low dose group, middle dose group, high dose group, positive drug control group, every group 10, low dose group, in
It is respectively 1mg, 2mg, 4mg, blank pair that dosage group, high dose group use the extracted coriolan composition of embodiment 3 every time
Coating substance is not given according to group, positive controls coat Bingpeng San, cavy is placed on fixed frame, earns opening with self-control opener
Chamber simultaneously pushes down tongue, and drug is applied on ulcer surface, every time administration guarantee drug oral cavity stop 15~20min, the morning, under
Noon each coating 1 time, continuous 7d before measurement record groups of animals administration, the 2nd, 4,6 day ulcer area size after medicine, is seen daily
Examine the ulcer healing number of animals of record each group.Compared with blank control group, with the significance of difference between t value method check groups.
3 coriolan composition of table to Candida albicans induce cavy canker sore therapeutic effect (N=10)
The data result of table 1~3 is shown, compared with blank control group, low dose group, middle dose group, high dose group burst
Ulcer area is obviously reduced, and the extracted coriolan composition Dichlorodiphenyl Acetate of embodiment 3, staphylococcus epidermis, Candida albicans lure
The cavy canker sore of hair has apparent therapeutic effect, and has dose dependent, and coriolan composition bursts to cavy oral cavity
The drug action of ulcer provides experimental data basis for the drug that coriolan composition prepares treatment canker sore.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and embodiment shown and described herein.
Claims (6)
1. the extracting method of the high coriolan composition of sugar content, which is characterized in that include the following steps：
Step 1: by rainbow conk and dry ice by weight 1.5~2.0:After 1 mixing, 120 mesh are crushed to pulverizer hereinafter, obtaining cloud
The sodium-chloride water solution that rainbow conk powder mass fraction is 0.65~0.85% is impregnated 30~40min, at 65~75 DEG C by sesame powder
Heated sealed handles 5~10min, filters to take filter residue I, and pretreatment liquid is added into filter residue I and impregnates 2~4h, in 8~10MPa's
20~30min of slightly boiled processing, filters to take filter residue II under pressure, and the mass fraction of 5~12 times of volumes is added into filter residue II every time
For 75~85% ethanol water 2~3h of refluxing extraction, refluxing extraction 2~3 times, filtering take filter residue III altogether, wherein by weight
Part meter is measured, pretreatment liquid includes：0.2~0.4 part of ursolic acid, 0.1~0.2 part of hesperidin methyl, 0.4~0.6 part of patchouli alcohol,
4~6 parts of guanidinium isothiocyanates, 1~2 part of lauryl sodium sulfate, 0.5~1 part of sodium citrate, 0.5~1.5 portion of peppermint oil, 0.6~
0.8 part of gaultherolin, 0.8~1 part of Monoolein, 1~1.5 part of dodecyl trimethyl ammonium chloride, 2~3 parts of chloroforms,
5~8 parts of isoamyl alcohol, 10~15 parts of ethyl alcohol；
Step 2: distilled water 2~3h of refluxing extraction of 8~12 times of volumes is added into filter residue III every time, total refluxing extraction 3~4
It is secondary, filter to take filtrate；
Step 3: the filtrate in step 2 is concentrated into the medicinal extract that relative density is 1.1~1.3 at 60 DEG C, ethyl alcohol, centrifugation is added
Take precipitating；
Step 4: precipitating is successively used ethyl alcohol, acetone washing, 2~4 times of distilled water of the precipitating after washing are dissolved, crosses and retains
The hollow-fibre membrane that molecular weight is 1,000,000 collects concentrate I, and concentrate I is crossed the doughnut that molecular cut off is 1,500,000
Film collects concentrate II, is centrifuged, and filtering takes filtrate；
Step 5: being spray-dried the filtrate in step 4 to get coriolan composition.
2. the extracting method of the high coriolan composition of sugar content as described in claim 1, which is characterized in that in step 3
Be added ethyl alcohol concrete operations be：The volume content that ethyl alcohol is added to ethyl alcohol into medicinal extract is 80~85%.
3. the extracting method of the high coriolan composition of sugar content as described in claim 1, which is characterized in that in step 3
The revolving speed of centrifugation is 2500~3000r/min.
4. the extracting method of the high coriolan composition of sugar content as described in claim 1, which is characterized in that in step 4
The weight of ethyl alcohol is 4~6 times of precipitating, and the weight of acetone is 3~6 times of precipitating.
5. the extracting method of the high coriolan composition of sugar content as described in claim 1, which is characterized in that in step 5
The time of spray drying is 8~12h.
6. the rainbow conk that the extracting method of the high coriolan composition of sugar content as claimed in any one of claims 1 to 5 obtains
Application of the polysaccharide composition in preparation treatment stomatocace medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811083756.1A CN108912240A (en) | 2018-09-17 | 2018-09-17 | The extracting method and its application of the high coriolan composition of sugar content |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811083756.1A CN108912240A (en) | 2018-09-17 | 2018-09-17 | The extracting method and its application of the high coriolan composition of sugar content |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108912240A true CN108912240A (en) | 2018-11-30 |
Family
ID=64409426
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811083756.1A Withdrawn CN108912240A (en) | 2018-09-17 | 2018-09-17 | The extracting method and its application of the high coriolan composition of sugar content |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108912240A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109463465A (en) * | 2018-12-29 | 2019-03-15 | 南阳师范学院 | A kind of preparation process of hickory chick bean curd stick |
| CN113520936A (en) * | 2021-06-24 | 2021-10-22 | 贵州苗老藤生物养生有限公司 | Preparation of honeysuckle extract and application of honeysuckle extract in toothpaste |
| CN117362465A (en) * | 2023-10-26 | 2024-01-09 | 河北葵克生物科技有限公司 | Method for extracting okra polysaccharide from okra |
-
2018
- 2018-09-17 CN CN201811083756.1A patent/CN108912240A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109463465A (en) * | 2018-12-29 | 2019-03-15 | 南阳师范学院 | A kind of preparation process of hickory chick bean curd stick |
| CN109463465B (en) * | 2018-12-29 | 2022-05-06 | 南阳师范学院 | Preparation process of morchella dried beancurd sticks |
| CN113520936A (en) * | 2021-06-24 | 2021-10-22 | 贵州苗老藤生物养生有限公司 | Preparation of honeysuckle extract and application of honeysuckle extract in toothpaste |
| CN117362465A (en) * | 2023-10-26 | 2024-01-09 | 河北葵克生物科技有限公司 | Method for extracting okra polysaccharide from okra |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2011153937A1 (en) | Herbe dendrobii extract, its preparation method and use thereof | |
| CN108912240A (en) | The extracting method and its application of the high coriolan composition of sugar content | |
| CN102816251B (en) | Preparation method of high-activity astragalus polysaccharide and pharmaceutical composition of high-activity astragalus polysaccharide | |
| CN112190593B (en) | Polysaccharide composition for inhibiting TRPV1 pathway and preparation method and application thereof | |
| CN103405639B (en) | Phellinus igniarius polyose and traditional Chinese medicine compound blood-glucose lowering oral liquid and preparation method thereof | |
| CN105434978A (en) | Polygonatum rhizome tea | |
| CN106883303A (en) | A kind of preparation method and application of geranium wilfordii polysaccharide and oligosaccharide | |
| CN109503729A (en) | A method of extracting polysaccharide | |
| CN106983786B (en) | A kind of Chinese medicine composition for preventing and/or treating hyperlipemia and preparation method thereof | |
| CN104844727A (en) | Rose acidic pectic polysaccharide and preparation method and application thereof | |
| CN103446201B (en) | A kind of composition for preventing and treating anaphylactia and preparation method thereof | |
| CN107412254A (en) | The application of anoectochilus roxburghii polyose extract | |
| CN110548075A (en) | Preparation method of veterinary ephedra, apricot kernel, gypsum and licorice oral liquid | |
| CN103550129A (en) | Plant composite polysaccharide and preparation method and application thereof | |
| CN103816210A (en) | Pueraria lobata extract and application thereof | |
| CN100509856C (en) | Cryptoporus volvatus polysaccharide, preparation and application thereof | |
| CN114392219B (en) | Peony extract composition with soothing effect and preparation method and application thereof | |
| CN100335073C (en) | Process for preparing semen momordicae seed extract containing triterpene saponin component | |
| CN110772554B (en) | Sodium hyaluronate gel for treating chloasma and preparation method thereof | |
| CN112472778A (en) | Ivory root extract, preparation method of pharmaceutical composition of ivory root extract and antimalarial application of ivory root extract | |
| CN102755266A (en) | Chinese herbal medicine extract, preparation method thereof, and application thereof | |
| CN108969580B (en) | Preparation method and application of blue cloth total tannin | |
| CN103848918A (en) | Extraction method for astragalus polysaccharide | |
| CN102961443B (en) | Rhizoma menispermi antineoplastic extract, Preparation method and use | |
| CN101129604B (en) | Antihypelipidemic traditional Chinese medicine producing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20181130 |