CN103816210A - Pueraria lobata extract and application thereof - Google Patents
Pueraria lobata extract and application thereof Download PDFInfo
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- CN103816210A CN103816210A CN201210464447.5A CN201210464447A CN103816210A CN 103816210 A CN103816210 A CN 103816210A CN 201210464447 A CN201210464447 A CN 201210464447A CN 103816210 A CN103816210 A CN 103816210A
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Abstract
The invention provides a pueraria lobata extract which is applied to drugs for preventing and treating bone loss diseases. The extract has the function of well inhibiting osteoclast forming and can be used for preparing the drugs for treating bone loss type diseases such as osteoporosis. The extract is obtained through the following steps: performing reflux extraction on dried pueraria lobata through ethanol, recovering ethanol to obtain extractum, dissolving the extractum by using distilled water and then leaching by using an organic solvent, and recovering the solvent after the extract is dissolved by ethanol. The pueraria lobata extract provided by the invention has the function of well inhibiting osteoclast forming, is from natural medicines, has few side effects and is safe in use.
Description
Technical field
The present invention relates to Radix Puerariae extract, especially relate to the application of Radix Puerariae extract in the medicine of preparation prevention and treatment bone loss disease.
Background technology
Along with world population aging becomes clear day by day, the sickness rate of osteoporosis raises year by year, has directly been affected the life quality of middle-aged and elderly people by the osteodynia due to osteoporosis and fracture, has become severe social problem.Human skeleton, by continuous osteogenesis and bone resorption, is being transformed always, and this transformation is transformed in bone.Keeping good balance in osteogenesis at an early age and bone resorption, but with advancing age, because this balance of a variety of causes is tended to bone resorption, the words that continue for a long time this state can make osseous tissue become fragile, and cause occurring the various osteopathias such as osteoporosis, fracture.In human body, osteoclast is the unique cell that has bone resorption.Research shows, when osteoclast formation becomes Showed Very Brisk, will cause the various osteopathias such as the above-mentioned osteoporosis (osteoporosis) causing due to bone loss.Therefore, formation and the bone resorption function of inhibition osteoclast are a kind of methods of very effective treatment osteoporosis.
Developed at present the prevention of multiple inhibition osteoclast formation and medicine and the method for the treatment of osteoporosis, but high owing to having price, long-term taking causes multiple side effect, thereby has certain limitation on using.Compared with synthetic new drug, natural medicinal ingredients has safety, inexpensive advantage.
Summary of the invention
The object of the present invention is to provide a kind of natural drug that derives from, there is the plant extract in the medicine that suppresses osteoclast formation, is applied to preparation prevention and treatment bone loss disease.
Suppressing osteoclast is the effective way of the bone loss diseases such as treatment osteoporosis.Applicant of the present invention finds that the extract of Radix Puerariae has the effect of good inhibition osteoclast formation, can be applied to the medicine of preparation prevention and treatment bone loss disease.
The invention provides the application of Radix Puerariae extract in the medicine of preparation prevention and treatment bone loss disease.
The present invention further provides the application of Radix Puerariae extract in the medicine of preparing prevention and treatment osteoporosis, rheumatoid arthritis.
Described Radix Puerariae extract has the effect of good inhibition osteoclast formation.
Described in also providing, the present invention there is the Radix Puerariae extract extracting method that suppresses osteoclast formation:
1. get dry Radix Puerariae, be ground into the fritter of 1-10mm, for subsequent use;
2. by the Radix Puerariae of pulverizing alcohol reflux 1-3 time, cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, obtains ethanol extract;
3. ethanol extract is added to distilled water thermosol, then use organic solvent extraction, decompression and solvent recovery, obtains extractum I;
4. the dissolve with ethanol of extractum I doubly being measured with 2-10, cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, must suppress the Radix Puerariae extract of osteoclast formation.
In preferred extracting method, at the step alcoholic solution that 1. described ethanol is 60-80%.
In preferred extracting method, step 2. described organic solvent be the alcohol that contains 1-6 carbon atom, alkane or esters.
In preferred extracting method, extract the mixture that organic solvent used is one or more organic solvents.
In preferred extracting method, at the step alcoholic solution that 3. described ethanol is 30-100%.
Described organic solvent extraction, can adopt by the little method extracting successively to large order of solvent polarity, or by a kind of method of solvent-extracted method or two or more mixed extractant solvent.
Radix Puerariae is the root of legume pueraria lobata, is that the one of some provinces and regions of southern china is often eaten vegetable, and its sweet in the mouth is cool good to eat, has heat clearing away, pathogenic fire reducing, all effects of toxin expelling.In Radix Puerariae, be rich in nearly 13 kinds of isoflavone like substance, comprise puerarin, Miroestrol., puerarin xyloside, daidzein etc., puerarin is effective in cure to hypertension, hyperlipidemia, hyperglycemia and cardiovascular and cerebrovascular disease.Radix Puerariae water decoction, alcohol extractum, total flavones and puerarin all have the effect of obvious expansion coronary vasodilator, and can make to be preced with blood flow increases, and vascular resistance reduces, and Radix Puerariae total flavones and puerarin energy decreased heart rate reduce heart total peripheral resistance, reduce myocardial oxygen consumption; Improve myocardium work efficiency
Though Radix Puerariae has above-mentioned multiple efficacies and effective ingredient, and coordinate the treatment of other Chinese herbal medicine for osteoporosis, still there is no to have in clear and definite Radix Puerariae the effective ingredient of osteoporosis disease.The present invention's multi-solvents extracting method, osteoclast formation Radix Puerariae extract is inhibited.Suppressing osteoclast is the effective way for the treatment of osteoporosis.
Radix Puerariae extract of the present invention has the effect of good inhibition osteoclast formation, and derives from natural drug, and few side effects uses safety.
Radix Puerariae extract of the present invention, extracting method is simple, is easy to suitability for industrialized production.
The specific embodiment
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.
Embodiment 1
1. get dry Radix Puerariae, be ground into the fritter of 1-10mm, for subsequent use;
2. by the Radix Puerariae crushing, with 3 times of 70% alcohol reflux 2 times, each 1.5 hours, merge extractive liquid,, cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, obtains ethanol extract;
3. ethanol extract is added to the distilled water thermosol of 5 times of amounts, then use successively petroleum ether, ethyl acetate, n-butanol extraction;
4. by acetic acid ethyl acetate extract, decompression and solvent recovery, obtains extractum I;
5. 90% dissolve with ethanol by 5 times of amounts by extractum I, cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, must suppress the Radix Puerariae extract of osteoclast formation.
Extract is added to pharmaceutical carrier, mix, granulate, dry, obtain granule.This granule can be used for the treatment of bone loss disease, as osteoporosis, and rheumatoid arthritis etc.
Embodiment 2
The Radix Puerariae extract that adopts embodiment 1 to obtain, measures its impact on osteoclast formation.
The formation system of osteoclast: adopt RAW264.7 cell under RANKL exists, cultivate the osteoclast that just can be formed as multinuclear for 3-5 days in α-MEM culture fluid.Tartrate resistant acid phosphatase for osteoclast (TRAP) staining forming is observed the positive apocyte of TRAP, and several 3 positive apocytes of above nuclear TRAP under the microscope, as the index of osteoclast formation.
Described RAW264.7 is the strain of cell mouse Monocytes/Macrophages, can be formed as the osteoclast of bone resorption ability under the existence of the osteoclast formation factor (RANKL).
Radix Puerariae extract is dissolved in α-MEM culture fluid, be mixed with finite concentration, then join in the osteoclast formation system of RAW264.7 cell, in the cell culture incubator of 5% carbon dioxide and 37 ℃, cultivate 3 days, the osteoclast obtaining is sent out dyeing with TRAP dyeing, and statistics has 3 positive osteoclasts of above nuclear TRAP under the microscope, the results are shown in Table 1.
The impact of table 1. Radix Puerariae extract on osteoclast formation
In the test of table 1: RAW264.7 cell is with 5 × 10
3the concentration of/well is inoculated in 96 orifice plates, in the α-MEM culture fluid that is 10ng/ml, cultivates 3 in RANKL concentration.
In table, the concentration of Radix Puerariae extract is the ultimate density in cell culture fluid, and each concentration is established 3 holes, and osteoclast number is the average in 3 holes.
In table, matched group is the solvent using in the time that Radix Puerariae extract concentration is 10ug/ml, as solvent control.
In table, suppression ratio is the suppression ratio of Radix Puerariae extract to osteoclast formation calculating with respect to matched group.
The result of table 1 is known, and Radix Puerariae extract significantly suppresses the formation of osteoclast, along with its suppression ratio of increase of concentration also increases.
Claims (7)
1. the Radix Puerariae extract application in the medicine of preparation prevention and treatment bone loss disease.
2. the Radix Puerariae extract application in the medicine of preparation prevention and treatment osteoporosis, rheumatoid arthritis.
3. according to the application of the Radix Puerariae extract described in claim 1 and 2, it is characterized in that, described Radix Puerariae extract extracts and obtains with the following method:
1. get dry Radix Puerariae, be ground into the fritter of 1-10mm, for subsequent use;
2. by the Radix Puerariae of pulverizing alcohol reflux 1-3 time, extracting solution cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, obtains ethanol extract;
3. ethanol extract is added to distilled water thermosol, then use organic solvent extraction, decompression and solvent recovery, obtains extractum I;
4. the dissolve with ethanol of extractum I doubly being measured with 2-10, cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, must suppress the Radix Puerariae extract of osteoclast formation.
4. the application of Radix Puerariae extract according to claim 3, is characterized in that: at the step alcoholic solution that 1. described ethanol is 60-80%.
5. the application of Radix Puerariae extract according to claim 3, is characterized in that: step 2. described organic solvent be the alcohol that contains 1-6 carbon atom, alkane or esters.
6. the application of Radix Puerariae extract according to claim 3, is characterized in that: extract the mixture that organic solvent used is one or more organic solvents.
7. the application of Radix Puerariae extract according to claim 3, is characterized in that: at the step alcoholic solution that 3. described ethanol is 30-100%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210464447.5A CN103816210A (en) | 2012-11-16 | 2012-11-16 | Pueraria lobata extract and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210464447.5A CN103816210A (en) | 2012-11-16 | 2012-11-16 | Pueraria lobata extract and application thereof |
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| CN103816210A true CN103816210A (en) | 2014-05-28 |
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| CN201210464447.5A Pending CN103816210A (en) | 2012-11-16 | 2012-11-16 | Pueraria lobata extract and application thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104983783A (en) * | 2015-07-20 | 2015-10-21 | 柳州蓓蒂芬科技有限公司 | Method for preparing plant extract |
| CN105168326A (en) * | 2015-06-24 | 2015-12-23 | 黄红林 | Radix puerariae extract preparation method |
| CN105168328A (en) * | 2015-09-07 | 2015-12-23 | 宜诺(天津)医药工程有限公司 | Kudzuvine root extract, extracting method and dropping pill of kudzuvine root extract |
| CN107432889A (en) * | 2016-05-26 | 2017-12-05 | 株式会社Lg生活健康 | Menopause symptom or osteoporosis improvement composition |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1321500A (en) * | 2001-04-23 | 2001-11-14 | *** | Method for preventing and curing diseases by using kakonein preparation |
| CN1391910A (en) * | 2001-06-20 | 2003-01-22 | 曹国华 | Process for preparing compound Chinese herbal medicine extract |
| CN1415291A (en) * | 2002-11-11 | 2003-05-07 | 上海交大昂立股份有限公司 | Product for preventing and curing osteoporosis and its preparation method |
| CN102319282A (en) * | 2011-09-21 | 2012-01-18 | 武汉工程大学 | Aqueous two-phase extraction method for total flavones in kudzu root |
-
2012
- 2012-11-16 CN CN201210464447.5A patent/CN103816210A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1321500A (en) * | 2001-04-23 | 2001-11-14 | *** | Method for preventing and curing diseases by using kakonein preparation |
| CN1391910A (en) * | 2001-06-20 | 2003-01-22 | 曹国华 | Process for preparing compound Chinese herbal medicine extract |
| CN1415291A (en) * | 2002-11-11 | 2003-05-07 | 上海交大昂立股份有限公司 | Product for preventing and curing osteoporosis and its preparation method |
| CN102319282A (en) * | 2011-09-21 | 2012-01-18 | 武汉工程大学 | Aqueous two-phase extraction method for total flavones in kudzu root |
Non-Patent Citations (5)
| Title |
|---|
| 梁敬钰主编: "《天然药物化学实验与指导》", 30 September 2010, 中国医药科技出版社 * |
| 王艳梅等: "黄豆普元的药理作用及临床应用", 《中国药师》 * |
| 臧洪敏等: "葛根素对成骨细胞生物学作用的实验研究", 《中国中药杂志》 * |
| 郑高利等: "葛根异黄酮对去卵巢大鼠骨矿密度和骨强度的影响", 《中草药》 * |
| 黄彤等: "葛根及葛根异黄酮的应用", 《中国食物与营养》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105168326A (en) * | 2015-06-24 | 2015-12-23 | 黄红林 | Radix puerariae extract preparation method |
| CN104983783A (en) * | 2015-07-20 | 2015-10-21 | 柳州蓓蒂芬科技有限公司 | Method for preparing plant extract |
| CN105168328A (en) * | 2015-09-07 | 2015-12-23 | 宜诺(天津)医药工程有限公司 | Kudzuvine root extract, extracting method and dropping pill of kudzuvine root extract |
| CN107432889A (en) * | 2016-05-26 | 2017-12-05 | 株式会社Lg生活健康 | Menopause symptom or osteoporosis improvement composition |
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Application publication date: 20140528 |