CN108904387B - Skin-care matrix capable of resisting exogenous stress and protecting DNA (deoxyribonucleic acid), and preparation method and application thereof - Google Patents

Abstract

The invention discloses a skin-care matrix for resisting exogenous stress and protecting DNA, and a preparation method and application thereof. The skin care matrix comprises the following components in percentage by mass: 10-20% of 1, 3-butanediol, 5-30% of purslane extract, 5-30% of artemia salina extract, 1-5% of poly gamma-sodium glutamate, 0.5-2% of sodium hyaluronate and the balance of water. The skin care matrix achieves good anti-inflammation and anti-photoaging effects through the synergistic interaction of the functional components of the purslane extract and the artemia salina extract, effectively reduces the generation of wrinkles and the elasticity loss of skin, improves fine lines of the skin, and has the effect of protecting DNA. Meanwhile, the preparation method of the skin-care matrix provided by the invention is simple, the operation is convenient, the active ingredients are fully released, the final skin-care matrix product with uniform property and stable quality can be ensured, and the preparation method is scientific and standard, is easy to operate, and is suitable for standardized production, popularization and application.

Description

Skin-care matrix capable of resisting exogenous stress and protecting DNA (deoxyribonucleic acid), and preparation method and application thereof

Technical Field

The invention relates to the technical field of cosmetics, in particular to a skin-care matrix for resisting exogenous stress and protecting DNA, and a preparation method and application thereof.

Background

With the continuous emergence of novel chemical substances and the popularization of daily chemicals, environmental problems such as ozone layer damage, haze, sand storm and the like occur frequently, the effect of external environmental factors is continuously enhanced, the skin becomes more sensitive and fragile, the function of the skin is weakened or lost, and the skin problem occurs. Research shows that ozone can generate oxidation pressure, destroy skin barrier to cause inflammation and reduce skin barrier function greatly. Inflammation causes many skin problems, such as redness, itching, dark circles under the eyes, bags under the eyes, etc. Also, there are some biological factors causing skin inflammation, such as parasites or insects such as cryptoptera, mites, etc., plants such as sumac or castor, bacteria, viruses, etc., and thus, inhibiting the occurrence of inflammation is one of means for resisting exogenous stress. The skin of the photoaging part is yellow or gray yellow and rough, wrinkles cannot disappear when the skin is stretched by force, abnormal pigmentation such as pigment spots or similar age spots appears after a long time, and even pigment maladjustment phenomenon with uneven shade appears. Long-term sunlight irradiation can also induce a series of proliferative diseases, such as seborrheic keratosis, papulopapule, light granuloma, solar keratosis, and the like, and severe cases can generate malignant tumors such as skin cancer.

Photoaging also brings harm to the DNA level, and genetically ultraviolet rays can enable two adjacent pyrimidines to be mutually crosslinked to form pyrimidine dimers, so that the three-dimensional conformation of DNA is obviously changed, and DNA replication and transcription of damaged parts are blocked. The most predominant (about 90 parts) photoproduct of DNA damage is Cyclobutane Pyrimidine Dimer (CPD), which can be repaired by a mechanism effective in skin cells, but the formation and accumulation of CPD can lead to a series of biological effects, such as skin inflammation, immunosuppression, and even skin tumors and skin cancers.

Therefore, the skin-care matrix capable of effectively resisting exogenous stress and protecting DNA is very important for inhibiting skin inflammation and resisting photoaging.

Disclosure of Invention

The invention aims to overcome the defects and shortcomings of the prior art, provides a skin-care matrix for resisting exogenous stress and protecting DNA, and achieves good skin-care effects of resisting inflammation, resisting aging and resisting DNA damage through the synergistic effect of a purslane extract and an artemia salina extract under a specific compounding condition.

The invention also aims to provide a preparation method of the skin care matrix.

The invention also aims to provide the application of the skin care matrix in preparing cosmetics.

It is still another object of the present invention to provide a cosmetic.

The invention also aims to provide a preparation method of the cosmetic.

The above purpose of the invention is realized by the following technical scheme:

a skin care matrix for resisting exogenous stress and protecting DNA comprises the following components in percentage by mass:

10-20% of 1, 3-butanediol, 5-30% of purslane extract, 5-30% of artemia salina extract, 1-5% of poly gamma-sodium glutamate, 0.5-25% of sodium hyaluronate and the balance of water.

The main functional component of the artemia salina extract is diguanosine tetraphosphate which is derived from a submarine plankton, the diguanosine tetraphosphate can be quickly converted into ATP due to the particularity of the chemical structure, and the artemia salina extract extracted from the plankton can supplement energy for skin, promote metabolism, repair and protect DNA and resist exogenous pressure for the skin.

Purslane contains high amounts of norepinephrine (0.25 parts in fresh purslane), neurohormones (with the ability to exert vasoconstrictive effects, resist hypotension, and reduce tissue bleeding), and purslane also contains many common nutrients including: vitamins (vitamin A, vitamin B1, vitamin B2, vitamin C, niacinamide, niacin, (alpha-tocopherol, beta-carotene, etc.), minerals, in particular potassium, fatty acids (in particular linolenic acid, the concentration in purslane being the highest in all leafy plants), glutathione, glutamic acid and aspartic acid, other ingredients include mucilages composed of a portion of acids and neutrals of known structure, calcium oxalate, malic and citric acids, dopamine and dopa, coumarins, flavonoids, saponins, and other useful substances such as urea. Vitamin C, B1, B2, PP; salts of Ca, Mg, Na, K; an organic acid; nicotinic (alkali) acid and oxalic acid; norepinephrine and biflavonoid liquiritin, and the purslane extract is an anti-inflammatory, anti-irritant and anti-allergy active substance in the cosmetics.

Poly-gamma-sodium glutamate is sold as AQUA MAX LM/Bio-PGA Na Powder, purchased from Japan, is put into yellow solid Powder, has excellent moisturizing effect, increases skin matrix protein, balances skin immunoreaction and can reduce photoaging.

The skin care matrix provided by the invention selects four functional components of poly-gamma-sodium glutamate, sodium hyaluronate, purslane extract and artemia extract, is matched with butanediol and water, and through reasonable compatibility of the components, a remarkable synergistic effect is generated, so that exogenous pressure is effectively resisted, inflammation and photoaging are resisted, generation of wrinkles and elastic loss of skin are effectively reduced, fine lines of the skin are improved, and the skin care matrix has the effect of protecting DNA.

Preferably, the composition comprises the following components in percentage by mass: 10-20% of 1, 3-butanediol, 10-30% of purslane extract, 15-30% of artemia salina extract, 1-5% of poly gamma-sodium glutamate and 0.5-2% of sodium hyaluronate.

Preferably, the following components are included in parts by weight: 15% of 1, 3-butanediol, 30% of purslane extract, 30% of artemia salina extract, 4% of poly gamma-sodium glutamate, 1% of sodium hyaluronate and 34% of water.

Preferably, the artemia salina extract is yellow liquid, the pH value is 5.0-6.0, and the specific gravity is 1.00-1.02.

The artemia salina extract is sold as GP4G, is purchased from Askaline Americana, and is quickly converted into ATP after entering human bodies, supplements energy instantly, can activate various physiological activities and metabolism of skin cells, and has the functions of DNA protection and repair.

Preferably, the masson grass extract is light yellow liquid, the pH value is 4.0-6.0, and the specific gravity is 1.000-1.040. The purslane extract is commercially available as Portulaca oleracea extract, and is available from BIOLAND corporation as a light yellow liquid, having a pH of 4.0-6.0 and a specific gravity of 1.000-1.040.

A preparation method of the skin care matrix comprises the following steps:

s1, mixing 1, 3-butanediol, poly gamma-sodium glutamate and sodium hyaluronate, stirring and heating until the components are completely dissolved, and keeping the temperature to obtain clear and transparent liquid;

s2, cooling the clear transparent liquid in the step S1 to 30-45 ℃, adding the masson grass extract and the artemia salina extract, and uniformly stirring to obtain the skin care matrix.

The preparation method of the skin-care matrix is simple and convenient to operate, the effective components of the components can be fully and uniformly mixed by heating, stirring and separating, the active components can be fully released, and the skin-care matrix product with uniform property and stable quality can be ensured to be finally obtained.

The components in S1 and S2 must be added separately, because poly-gamma-sodium glutamate and sodium hyaluronate need to be dissolved stably, 1, 3-butanediol is a raw material for dispersing the components, and therefore the poly-gamma-sodium glutamate and the sodium hyaluronate need to be added at a high temperature, and the purslane extract and the artemia extract cannot bear the high temperature, and need to be added after being cooled to a proper temperature, and need to be cooled.

Preferably, the heating temperature in S1 is 75-95 ℃, and the heat preservation time is 15-30 min. The heat preservation allows the material to be completely dissolved.

Preferably, the stirring speed in S1 and S2 is 100-500 rpm, and the stirring time is 15-30 min. Agitation allows for more uniform mixing between the components.

Preferably, the method comprises the following steps:

s1, mixing 1, 3-butanediol, poly gamma-sodium glutamate and sodium hyaluronate, heating to 85 ℃, stirring at 300rpm until the materials are completely dissolved, and keeping the temperature for 20min to obtain clear and transparent liquid;

s2, cooling the clear transparent liquid in the step S1 to 45 ℃, adding the masson grass extract and the artemia salina extract, stirring at 300rpm for 20min, and stirring uniformly to obtain the skin care matrix.

The application of the skin care matrix in preparing cosmetics is also within the protection scope of the invention. The skin care matrix can be used for preparing astringent, essence, lotion, cream and other products used in beauty industry.

Preferably, the mass percent of the skin care matrix in the cosmetic is 10-25%.

The cosmetic comprises the following components in percentage by mass:

phase A: 0.5-3% of glyceryl stearate, 0.5-3% of PEG-100 stearate, 2-5% of polydimethylsiloxane, 3-6% of dioctyl carbonate, 1-4% of caprylic/capric triglyceride, 0.1-0.5% of cetyl alcohol, 1-2% of stearyl alcohol, 0.1-0.5% of hydrogenated lecithin and 0.05-0.3% of propyl hydroxybenzoate;

phase B: 5-15% of glycerin, 0.1-0.5% of carbomer, 0.05-0.2% of EDTA disodium, 0.1-0.6% of methylparaben and the balance of water;

and C phase: 0.1-0.5% of aminomethyl propanol;

phase D: 0.01-0.035% of essence and 10-25% of the matrix.

A preparation method of the cosmetic comprises the following steps:

s3, heating and stirring the phase A and the phase B to 75-90 ℃ respectively, mixing to obtain a mixture, homogenizing, vacuumizing, and preserving heat;

s4, cooling the mixture in the S3 to 60-70 ℃, adding the phase C, homogenizing, vacuumizing and stirring;

s5, cooling the product of the S4 to 30-45 ℃, adding the phase D, vacuumizing, and stirring to obtain the cosmetic.

The phase A of the cosmetic provided by the invention is an oil phase and a surfactant, the phase B is a water phase, a thickening agent and a humectant, and the phase C is a pH regulator, so that the cosmetic has a thickening effect after the pH is regulated. Preferably, the stirring speed is 100-500 rpm.

Preferably, the phase A and the phase B are heated, then are subjected to heat preservation for 10-30 min, and then are mixed.

Preferably, the homogenizing speed in S3 is 3000-5000 rpm, and the homogenizing time is 3-8 min.

Preferably, the heat preservation time in S3 is 10-30 min.

Preferably, the homogenizing speed of S4 is 1000-2000 rpm, and the time is 30-60S.

Preferably, the vacuum degree of the vacuumizing is-0.02 to-0.05 Mpa.

Preferably, the stirring time of S5 is 10-30 min.

More preferably, the preparation of the above cosmetic comprises the steps of:

s3, respectively heating and stirring the phase A and the phase B to 85 ℃, preserving heat for 20min, mixing to obtain a mixture, homogenizing at 3000rpm for 5min, vacuumizing to-0.03 mpa, preserving heat for 15min, and stirring at 500 rpm;

s4, stirring the mixture in the S3, cooling to 70 ℃, adding the phase C, homogenizing at 1000rpm for 30S, vacuumizing to-0.03 mpa, and stirring at 500 rpm;

s5, cooling the product of S4 to 45 ℃, adding the phase D, vacuumizing to-0.03 mpa, and stirring for 15min to obtain the cosmetic, wherein the stirring speed is 500 rpm.

Compared with the prior art, the invention has the beneficial effects that:

the invention provides a skin-care matrix for resisting exogenous stress and protecting DNA, which achieves good anti-inflammatory and anti-photoaging effects by the synergistic effect of four functional components, namely gamma-sodium glutamate, sodium hyaluronate, a purslane extract and an artemia extract, wherein the minimum release amount of interleukin 1 after 8-hour irradiation is 14.7, the maximum data of the photoaging resistance test can reach 90.2, the generation of wrinkles and the elastic loss of skin are effectively reduced, the fine lines of the skin are improved, the skin-care matrix has a remarkable effect on wrinkle reduction, and has the effect of protecting DNA, and the minimum percentage of DNA damage is 8%.

Meanwhile, the preparation method of the skin care matrix provided by the invention is simple and convenient to operate, can fully and uniformly mix the effective components of each component through heating, stirring and separation, is beneficial to fully releasing the active components, can ensure that a skin care matrix product with uniform property and stable quality can be finally obtained, is scientific and standard, is easy to operate, and is suitable for standardized production, popularization and application.

Detailed Description

The present invention will be further described with reference to specific embodiments, but the present invention is not limited to the examples in any way. The starting reagents employed in the examples of the present invention are, unless otherwise specified, those that are conventionally purchased.

Example 1

A skin care matrix for protecting DNA against exogenous stress, comprising the following components in parts by weight:

15% of 1, 3-butanediol, 2% of poly-gamma-sodium glutamate, 1% of sodium hyaluronate, 15% of Mayan extract, 15% of artemia salina extract and the balance of water.

The preparation method comprises the following steps:

s1, heating 1, 3-butanediol, poly gamma-sodium glutamate and sodium hyaluronate to 85 ℃, stirring at the speed of 300rpm until the materials are completely dissolved, and keeping the temperature for 20min to obtain clear and transparent liquid;

s2, cooling the clear transparent liquid in the step S1 to 45 ℃, adding the artemia salina extract and the masson grass extract, stirring at the speed of 300rpm for 20min, and uniformly stirring to obtain the skin care matrix.

An emulsion cosmetic comprises the following components in parts by weight:

phase A: 2.5% of glyceryl stearate, 2.5% of PEG-100 stearate, 4% of polydimethylsiloxane, 3% of dioctyl carbonate, 2% of caprylic/capric triglyceride, 0.5% of cetyl alcohol, 1% of stearyl alcohol, 0.3% of hydrogenated lecithin and 0.1% of propylhydroxybenzoate;

phase B: 10% of glycerin, 0.3% of carbomer, 0.05% of EDTA disodium, 0.2% of methylparaben and the balance of water;

and C phase: aminomethyl propanol 0.18%;

phase D: 0.02% of essence and 20% of matrix;

the preparation steps are as follows:

s3, respectively heating and stirring the phase A and the phase B to 85 ℃, preserving heat for 20min, mixing to obtain a mixture, homogenizing at 3000rpm for 5min, vacuumizing to-0.03 mpa, preserving heat for 15min, and stirring at 500 rpm;

s4, stirring the mixture in the S3, cooling to 70 ℃, adding the phase C, homogenizing at 1000rpm for 30S, vacuumizing to-0.03 mpa, and stirring at 500 rpm;

s5, cooling the product of S4 to 45 ℃, adding the phase D, vacuumizing to-0.03 mpa, and stirring for 15min to obtain the cosmetic, wherein the stirring speed is 500 rpm.

Example 2

A skin care matrix for resisting exogenous stress and protecting DNA comprises the following components in percentage by mass:

15% of 1, 3-butanediol, 2% of poly-gamma-sodium glutamate, 1% of sodium hyaluronate, 10% of Mayan extract, 20% of artemia extract and the balance of water.

The preparation method comprises the following steps:

s1, heating 1, 3-butanediol, poly gamma-sodium glutamate and sodium hyaluronate to 85 ℃, stirring at the speed of 300rpm until the materials are completely dissolved, and keeping the temperature for 20min to obtain clear and transparent liquid;

s2, cooling the clear transparent liquid in the step S1 to 45 ℃, adding the artemia salina extract and the masson grass extract, stirring at the speed of 300rpm for 20min, and uniformly stirring to obtain the skin care matrix.

An emulsion cosmetic comprises the following components in percentage by mass:

phase A: 2.5% of glyceryl stearate, 2.5% of PEG-100 stearate, 4% of polydimethylsiloxane, 3% of dioctyl carbonate, 2% of caprylic/capric triglyceride, 0.5% of cetyl alcohol, 1% of stearyl alcohol, 0.3% of hydrogenated lecithin and 0.1% of propylhydroxybenzoate;

phase B: 10% of glycerin, 0.3% of carbomer, 0.05% of EDTA disodium, 0.2% of methylparaben and the balance of water;

and C phase: aminomethyl propanol 0.18%;

phase D: 0.02% of essence and 20% of matrix;

the preparation steps are as follows:

s3, respectively heating and stirring the phase A and the phase B to 85 ℃, preserving heat for 20min, mixing to obtain a mixture, homogenizing at 3000rpm for 5min, vacuumizing to-0.03 mpa, preserving heat for 15min, and stirring at 500 rpm;

s4, stirring the mixture in the S3, cooling to 70 ℃, adding the phase C, homogenizing at 1000rpm for 30S, vacuumizing to-0.03 mpa, and stirring at 500 rpm;

s5, cooling the product of S4 to 45 ℃, adding the phase D, vacuumizing to-0.03 mpa, and stirring for 15min to obtain the cosmetic, wherein the stirring speed is 500 rpm.

Example 3

A skin care matrix for resisting exogenous stress and protecting DNA comprises the following components in percentage by mass:

15% of 1, 3-butanediol, 2% of poly-gamma-sodium glutamate, 1% of sodium hyaluronate, 20% of masson forage extract, 10% of artemia salina extract and the balance of water.

The preparation method comprises the following steps:

s1, heating 1, 3-butanediol, poly gamma-sodium glutamate and sodium hyaluronate to 85 ℃, stirring at the speed of 300rpm until the materials are completely dissolved, and keeping the temperature for 20min to obtain clear and transparent liquid;

s2, cooling the clear transparent liquid in the step S1 to 45 ℃, adding the artemia salina extract and the masson grass extract, stirring at the speed of 300rpm for 20min, and uniformly stirring to obtain the skin care matrix.

An emulsion cosmetic comprises the following components in percentage by mass:

phase A: 2.5% of glyceryl stearate, 2.5% of PEG-100 stearate, 4% of polydimethylsiloxane, 3% of dioctyl carbonate, 2% of caprylic/capric triglyceride, 0.5% of cetyl alcohol, 1% of stearyl alcohol, 0.3% of hydrogenated lecithin and 0.1% of propylhydroxybenzoate;

phase B: 10% of glycerin, 0.3% of carbomer, 0.05% of EDTA disodium, 0.2% of methylparaben and the balance of water;

and C phase: aminomethyl propanol 0.18%;

phase D: 0.02% of essence and 20% of matrix;

the preparation steps are as follows:

s3, respectively heating and stirring the phase A and the phase B to 85 ℃, preserving heat for 20min, mixing to obtain a mixture, homogenizing at 3000rpm for 5min, vacuumizing to-0.03 mpa, preserving heat for 15min, and stirring at 500 rpm;

s4, stirring the mixture in the S3, cooling to 70 ℃, adding the phase C, homogenizing at 1000rpm for 30S, vacuumizing to-0.03 mpa, and stirring at 500 rpm;

s5, cooling the product of S4 to 45 ℃, adding the phase D, vacuumizing to-0.03 mpa, and stirring for 15min to obtain the cosmetic, wherein the stirring speed is 500 rpm.

Example 4

A skin care matrix for resisting exogenous stress and protecting DNA comprises the following components in percentage by mass:

15% of 1, 3-butanediol, 2% of poly-gamma-sodium glutamate, 1% of sodium hyaluronate, 30% of Mayanfind extract, 30% of artemia salina extract and the balance of water.

The preparation method comprises the following steps:

s1, heating 1, 3-butanediol, poly gamma-sodium glutamate and sodium hyaluronate to 85 ℃, stirring at the speed of 300rpm until the materials are completely dissolved, and keeping the temperature for 20min to obtain clear and transparent liquid;

s2, cooling the clear transparent liquid in the step S1 to 45 ℃, adding the artemia salina extract and the masson grass extract, stirring at the speed of 300rpm for 20min, and uniformly stirring to obtain the skin care matrix.

An emulsion cosmetic comprises the following components in percentage by mass:

phase A: 2.5% of glyceryl stearate, 2.5% of PEG-100 stearate, 4% of polydimethylsiloxane, 3% of dioctyl carbonate, 2% of caprylic/capric triglyceride, 0.5% of cetyl alcohol, 1% of stearyl alcohol, 0.3% of hydrogenated lecithin and 0.1% of propylhydroxybenzoate;

phase B: 10% of glycerin, 0.3% of carbomer, 0.05% of EDTA disodium, 0.2% of methylparaben and the balance of water;

and C phase: aminomethyl propanol 0.18%;

phase D: 0.02% of essence and 20% of matrix;

the preparation steps are as follows:

s3, respectively heating and stirring the phase A and the phase B to 85 ℃, preserving heat for 20min, mixing to obtain a mixture, homogenizing at 3000rpm for 5min, vacuumizing to-0.03 mpa, preserving heat for 15min, and stirring at 500 rpm;

s4, stirring the mixture in the S3, cooling to 70 ℃, adding the phase C, homogenizing at 1000rpm for 30S, vacuumizing to-0.03 mpa, and stirring at 500 rpm;

s5, cooling the product of S4 to 45 ℃, adding the phase D, vacuumizing to-0.03 mpa, and stirring for 15min to obtain the cosmetic, wherein the stirring speed is 500 rpm.

Comparative example 1

A skin care matrix comprises the following components in percentage by mass:

15% of 1, 3-butanediol, 30% of purslane extract, 2% of poly gamma-sodium glutamate, 1% of sodium hyaluronate and the balance of water.

The preparation method comprises the following steps:

s1, heating 1, 3-butanediol, poly gamma-sodium glutamate and sodium hyaluronate to 85 ℃, stirring at the speed of 300rpm until the materials are completely dissolved, and keeping the temperature for 20min to obtain clear and transparent liquid;

s2, cooling the clear transparent liquid in the step S1 to 45 ℃, adding the masson grass extract, stirring at 300rpm for 20min, and uniformly stirring to obtain the skin care matrix.

An emulsion cosmetic comprising the following components in mass%:

phase A: 2.5% of glyceryl stearate, 2.5% of PEG-100 stearate, 4% of polydimethylsiloxane, 3% of dioctyl carbonate, 2% of caprylic/capric triglyceride, 0.5% of cetyl alcohol, 1% of stearyl alcohol, 0.3% of hydrogenated lecithin and 0.1% of propylhydroxybenzoate;

phase B: 10% of glycerin, 0.3% of carbomer, 0.05% of EDTA disodium, 0.2% of methylparaben and the balance of water;

and C phase: aminomethyl propanol 0.18%;

phase D: 0.02% of essence and 20% of matrix;

the preparation steps are as follows:

s3, respectively heating and stirring the phase A and the phase B to 85 ℃, preserving heat for 20min, mixing to obtain a mixture, homogenizing at 3000rpm for 5min, vacuumizing to-0.03 mpa, preserving heat for 15min, and stirring at 500 rpm;

s4, stirring the mixture in the S3, cooling to 70 ℃, adding the phase C, homogenizing at 1000rpm for 30S, vacuumizing to-0.03 mpa, and stirring at 500 rpm;

s5, cooling the product of S4 to 45 ℃, adding the phase D, vacuumizing to-0.03 mpa, and stirring for 15min to obtain the cosmetic, wherein the stirring speed is 500 rpm.

Comparative example 2

A skin care matrix comprises the following components in percentage by mass:

15% of 1, 3-butanediol, 30% of artemia extract, 2% of poly gamma-sodium glutamate and 1% of sodium hyaluronate.

The preparation method comprises the following steps:

s1, heating 1, 3-butanediol, poly gamma-sodium glutamate and sodium hyaluronate to 85 ℃, stirring at the speed of 300rpm until the materials are completely dissolved, and keeping the temperature for 20min to obtain clear and transparent liquid;

s2, cooling the clear transparent liquid in the step S1 to 45 ℃, adding the artemia salina extract, stirring at 300rpm for 20min, and stirring uniformly to obtain the skin care matrix.

An emulsion cosmetic comprises the following components in percentage by mass:

phase A: 2.5% of glyceryl stearate, 2.5% of PEG-100 stearate, 4% of polydimethylsiloxane, 3% of dioctyl carbonate, 2% of caprylic/capric triglyceride, 0.5% of cetyl alcohol, 1% of stearyl alcohol, 0.3% of hydrogenated lecithin and 0.1% of propylhydroxybenzoate;

phase B: 10% of glycerin, 0.3% of carbomer, 0.05% of EDTA disodium, 0.2% of methylparaben and the balance of water;

and C phase: aminomethyl propanol 0.18%;

phase D: 0.02% of essence and 20% of matrix;

the preparation steps are as follows:

s3, respectively heating and stirring the phase A and the phase B to 85 ℃, preserving heat for 20min, mixing to obtain a mixture, homogenizing at 3000rpm for 5min, vacuumizing to-0.03 mpa, preserving heat for 15min, and stirring at 500 rpm;

s4, stirring the mixture in the S3, cooling to 70 ℃, adding the phase C, homogenizing at 1000rpm for 30S, vacuumizing to-0.03 mpa, and stirring at 500 rpm;

s5, cooling the product of S4 to 45 ℃, adding the phase D, vacuumizing to-0.03 mpa, and stirring for 15min to obtain the cosmetic, wherein the stirring speed is 500 rpm.

Comparative example 3

A skin care matrix comprises the following components in percentage by mass:

15% of 1, 3-butanediol, 2% of poly gamma-sodium glutamate, 1% of sodium hyaluronate and the balance of water.

The preparation method comprises the following steps:

s1, heating 1, 3-butanediol, poly gamma-sodium glutamate and sodium hyaluronate to 85 ℃, stirring at the speed of 300rpm until the materials are completely dissolved, and keeping the temperature for 20min to obtain clear transparent liquid, namely the skin-care matrix.

An emulsion cosmetic comprising the following components in mass%:

phase A: 2.5% of glyceryl stearate, 2.5% of PEG-100 stearate, 4% of polydimethylsiloxane, 3% of dioctyl carbonate, 2% of caprylic/capric triglyceride, 0.5% of cetyl alcohol, 1% of stearyl alcohol, 0.3% of hydrogenated lecithin and 0.1% of propylhydroxybenzoate;

phase B: 10% of glycerin, 0.3% of carbomer, 0.05% of EDTA disodium, 0.2% of methylparaben and the balance of water;

and C phase: aminomethyl propanol 0.18%;

phase D: 0.02% of essence and 20% of matrix;

the preparation steps are as follows:

s3, respectively heating and stirring the phase A and the phase B to 85 ℃, preserving heat for 20min, mixing to obtain a mixture, homogenizing at 3000rpm for 5min, vacuumizing to-0.03 mpa, preserving heat for 15min, and stirring at 500 rpm;

s4, stirring the mixture in the S3, cooling to 70 ℃, adding the phase C, homogenizing at 1000rpm for 30S, vacuumizing to-0.03 mpa, and stirring at 500 rpm;

s5, cooling the product of S4 to 45 ℃, adding the phase D, vacuumizing to-0.03 mpa, and stirring for 15min to obtain the cosmetic, wherein the stirring speed is 500 rpm.

Comparative example 4

A skin care matrix for resisting exogenous stress and protecting DNA comprises the following components in percentage by mass:

15% of 1, 3-butanediol, 2% of poly-gamma-sodium glutamate, 1% of sodium hyaluronate, 40% of Mayan extract, 30% of artemia extract and the balance of water.

The preparation method is the same as in example 4.

An emulsion cosmetic comprises the following components in percentage by mass:

phase A: 2.5% of glyceryl stearate, 2.5% of PEG-100 stearate, 4% of polydimethylsiloxane, 3% of dioctyl carbonate, 2% of caprylic/capric triglyceride, 0.5% of cetyl alcohol, 1% of stearyl alcohol, 0.3% of hydrogenated lecithin and 0.1% of propylhydroxybenzoate;

phase B: 10% of glycerin, 0.3% of carbomer, 0.05% of EDTA disodium, 0.2% of methylparaben and the balance of water;

and C phase: aminomethyl propanol 0.18%;

phase D: 0.02% of essence and 20% of matrix;

the preparation procedure was the same as in example 4.

Result detection

1. Determination of inflammatory factor Interleukin 1 (IL-1) by anti-inflammatory assay-ELISA assay

Enzyme-linked immunosorbent assay (ELISA) is a technique in which a known antigen or antibody is adsorbed on the surface of a solid phase carrier, and an enzyme-labeled antigen-antibody reaction is carried out on the surface of the solid phase. The technology can be used for detecting macromolecular antigens, specific antibodies and the like, and has the advantages of rapidness, sensitivity, simplicity, convenience, easy standardization of carriers and the like. It adopts the specific reaction of antigen and antibody to connect the tested substance with enzyme, then makes the enzyme and substrate produce colour reaction for quantitative determination. The object to be measured may be an antibody or an antigen.

IL-1 is a cytokine produced by monocytes, endothelial cells, fibroblasts and other cell types in response to infection and is a measure of the inflammatory response of the human body.

This experiment measures the release of IL-1 by HaCaT cells after 8 hours under UVB irradiation at 30 mJ/cm2 to evaluate the anti-inflammatory efficacy of the product of each example, and the results are as follows:

TABLE 1 anti-inflammatory efficacy data sheet

From the experimental results of examples 1 to 4 and comparative examples 1 to 3, it is clear that, although the purslane extract and the artemia salina extract have slight anti-inflammatory effects when acting alone, the purslane extract and the artemia salina extract are matched for use, so that a better anti-inflammatory effect can be achieved, and the purslane extract and the artemia salina extract have a synergistic anti-inflammatory effect.

2. Photoaging resistance detection-UVA resistance test

The fiber cells have important significance in human body and are important influencing factors for skin elasticity and wrinkle generation. The UVA of the experiment with the wavelength of 320-400nm (the average wavelength of 365 nm) is 3J/cm²At an intensity of (c), the viability of human fibroblasts is tested (based on the viability of cells that normally do not receive UVA irradiation being 100). The experimental results are as follows:

TABLE 2 anti-photoaging data

From the experimental results of examples 1 to 4 and comparative examples 1 to 3, it is clear that although the purslane extract and the artemia salina extract have a slight anti-photoaging effect when acting alone, it is clear that the purslane extract and the artemia salina extract are used in combination to achieve a better anti-photoaging effect, and the purslane extract and the artemia salina extract really have a synergistic anti-photoaging effect.

3. DNA Damage test-comet test

Comet assay is also called single cell gel electrophoresis assay, and is a technology for distinguishing genotoxicity by detecting DNA chain damage, which is firstly proposed in 1984 by Ostling. It can effectively detect and quantitatively analyze the damage degree of DNA single-strand and double-strand gaps in cells. The more damaged the DNA, the more broken strands and fragments are produced, and the longer the DNA is, the more DNA is migrated under the same electrophoresis conditions, and the longer the migration distance is. Therefore, the degree of DNA damage is evaluated by the ratio between the size of the tail of the comet and the comet (undamaged DNA).

The experimental subject of the experiment is human fibroblast, and the concentration is 30 mJ/cm²UVB irradiation for 48 hours and comet assay, the percentage of DNA damage results are as follows:

TABLE 3 comet test DNA damage data

From the experimental results of examples 1 to 4 and comparative examples 1 to 3, it is clear that although the purslane extract and the artemia salina extract have a slightly small effect of protecting DNA when acting alone, it is clear that the purslane extract and the artemia salina extract are used in combination to achieve a better effect of protecting DNA, and the purslane extract and the artemia extract indeed have a synergistic effect of protecting DNA.

4. Anti-aging test

70 female volunteers (age 40-50 years, average age 44 years) were randomly and equally divided into 7 groups, and the emulsion cosmetics prepared in examples 1-7 were applied after cleansing the face every morning and evening, and the amount of each of the groups was the same, and the wrinkle area, the number of wrinkles, and the degree of reduction in the wrinkle depth in the forehead area were evaluated by using a VISIA instrument after 8 weeks, and the test results are shown in table 4.

TABLE 4 test results

The data of examples 1 to 4 and comparative examples 1 to 3 show that the purslane extract and the artemia salina extract are used in a matching manner, so that a better wrinkle-reducing and wrinkle-removing effect can be achieved, the artemia salina extract has a wrinkle-reducing and wrinkle-removing effect, but the wrinkle-reducing and wrinkle-removing effect is not obvious when the purslane extract and the artemia salina extract are used alone, so that synergistic anti-aging is really achieved, and the wrinkle-reducing and wrinkle-removing effect is more obvious.

The above-mentioned 40% of the extract of Odontoglossum and 30% of the extract of artemia salina in comparative example 4 were added in amounts of more than 60%, which were comparable to those of example 4 in anti-inflammatory, anti-photoaging, anti-DNA damage and anti-aging properties, and did not show significant improvement in function due to the increase in the effective components thereof, and when the amounts of the artemia salina extract and the purslane extract in the base exceeded 30% and the total amount thereof exceeded 60%, there was a sign of allergy in the population of subjects. Of the 10 subjects in comparative example 4, 2 had a fever or tingling sensation and 2 had reddened skin, whereas in examples 1-4, only one subject in example 4 had slightly reddened skin for the first use and continued use with no signs of allergy. Therefore, the excessive use of the artemia salina extract and the purslane extract in the substrate of the invention can cause skin allergy, and the reasonable compounding of the invention can generate good synergistic effect, so that the substrate of the invention not only has synergistic effect on the performances of anti-inflammation, anti-photoaging, anti-DNA damage, anti-aging and the like, but also can prevent the generation of side effects of allergy and the like.

It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims (9)

1. The skin-care matrix for resisting exogenous stress and protecting DNA is characterized by comprising the following components in percentage by mass:

10-20% of 1, 3-butanediol, 30% of purslane extract, 30% of artemia salina extract, 1-5% of poly gamma-sodium glutamate, 0.5-2% of sodium hyaluronate and the balance of water.

2. The skin care base of claim 1, wherein the artemia extract is a yellow liquid having a pH of 5.0 to 6.0 and a specific gravity of 1.00 to 1.02.

3. The skin care matrix of claim 1, wherein the mattes extract is a pale yellow liquid having a pH of 4.0 to 6.0 and a specific gravity of 1.000 to 1.040.

4. A method of making a skin care base according to claim 1 comprising the steps of:

s1, heating and mixing 1, 3-butanediol, poly gamma-sodium glutamate and sodium hyaluronate until the materials are completely dissolved to obtain clear transparent liquid, wherein the heating temperature is 75-95 ℃;

s2, cooling the clear transparent liquid in the step S1 to 30-45 ℃, adding the masson grass extract and the artemia salina extract, and uniformly mixing to obtain the skin care matrix.

5. The preparation method according to claim 4, wherein the heating in S1 is carried out for 15-30 min after the heating is completed and the solution is dissolved.

6. Use of a skin care base according to claim 1 for the preparation of a cosmetic product.

7. The use according to claim 6, wherein the cosmetic comprises 10 to 25% by weight of the skin care base.

8. The cosmetic is characterized by comprising the following components in percentage by mass:

phase A: 0.5-3% of glyceryl stearate, 0.5-3% of PEG-100 stearate, 2-5% of polydimethylsiloxane, 3-6% of dioctyl carbonate, 1-4% of caprylic/capric triglyceride, 0.1-0.5% of cetyl alcohol, 1-2% of stearyl alcohol, 0.1-0.5% of hydrogenated lecithin and 0.05-0.1% of propyl hydroxybenzoate;

phase B: 5-15% of glycerin, 0.1-0.5% of carbomer, 0.05-0.2% of EDTA disodium, 0.1-0.2% of methylparaben and the balance of water;

and C phase: 0.1-0.5% of aminomethyl propanol;

phase D: 0.01-0.03% of essence and 10-25% of the substrate of claim 1 or 2.

9. A method for preparing the cosmetic according to claim 8, comprising the steps of:

s3, respectively heating the phase A component and the phase B component to 75-90 ℃, mixing to obtain a mixture, homogenizing, stirring, vacuumizing, and preserving heat;

s4, cooling the mixture in the S3 to 60-70 ℃, adding the phase C, homogenizing, vacuumizing and stirring;

s5, cooling the product of the S4 to 30-45 ℃, adding the phase D, vacuumizing, and stirring to obtain the cosmetic.