CN108195900A - The electrochemical sensor of packed cell volume test function with temperature-compensating - Google Patents
The electrochemical sensor of packed cell volume test function with temperature-compensating Download PDFInfo
- Publication number
- CN108195900A CN108195900A CN201711366439.6A CN201711366439A CN108195900A CN 108195900 A CN108195900 A CN 108195900A CN 201711366439 A CN201711366439 A CN 201711366439A CN 108195900 A CN108195900 A CN 108195900A
- Authority
- CN
- China
- Prior art keywords
- cell volume
- packed cell
- temperature
- compensating
- electrode system
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000012360 testing method Methods 0.000 title claims abstract description 80
- 238000001514 detection method Methods 0.000 claims abstract description 21
- 239000000758 substrate Substances 0.000 claims abstract description 14
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 11
- 238000002347 injection Methods 0.000 claims abstract description 5
- 239000007924 injection Substances 0.000 claims abstract description 5
- 210000004369 blood Anatomy 0.000 claims description 29
- 239000008280 blood Substances 0.000 claims description 29
- 239000000463 material Substances 0.000 claims description 12
- 229910052799 carbon Inorganic materials 0.000 claims description 10
- 230000005518 electrochemistry Effects 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- -1 polyethylene terephthalate Polymers 0.000 claims description 6
- 229920000139 polyethylene terephthalate Polymers 0.000 claims description 6
- 239000005020 polyethylene terephthalate Substances 0.000 claims description 6
- 239000002390 adhesive tape Substances 0.000 claims description 4
- 229920002125 Sokalan® Polymers 0.000 claims description 3
- 239000004584 polyacrylic acid Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 101
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 17
- 239000008103 glucose Substances 0.000 description 16
- 239000012491 analyte Substances 0.000 description 15
- 210000003743 erythrocyte Anatomy 0.000 description 11
- 238000012937 correction Methods 0.000 description 7
- 238000005534 hematocrit Methods 0.000 description 6
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 2
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 229940116269 uric acid Drugs 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000002274 desiccant Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000013102 re-test Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/05—Investigating sedimentation of particle suspensions in blood
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to technical field of medical equipment, are related to a kind of electrochemical sensor of the packed cell volume test function with temperature-compensating.The electrochemical sensor includes substrate, electrode layer, reaction reagent, pad level and hydrophilic membrane；The injection port end of substrate is equipped with notch；Electrode layer includes correcting electrode system and detecting electrode system, and the two is in the same plane, and correcting electrode system includes a pair of of correcting electrode, and packed cell volume test is carried out by applying working volts alternating current；Detecting electrode system includes a pair of of detecting electrode, and sample introduction is analyzed by applying direct-current working volts, and object is tested；Pad level includes exhaust pad level, reaction pad level and detection zone.The invention also discloses a kind of temperature-compensation method to packed cell volume test, including the packed cell volume measured equation with temperature-compensating.The present invention is conducive to electrochemical sensor and accurate testing result is obtained within the temperature range of bigger, and rational in infrastructure, simple for process, is conducive to manufacture on a large scale.
Description
Technical field
The invention belongs to technical field of medical equipment, are related to detecting the electrochemical sensor of analyte in blood sample, tool
Body is related to a kind of electrochemical sensor of the packed cell volume test function with temperature-compensating.
Background technology
At present, electrochemical sensor is widely used, and be gradually popularized for domestic consumer in detection (POCT) immediately, people
The content of analyte in blood sample (such as glucose, uric acid, cholesterol etc.) can be easily monitored, whether testing result is accurate
Really reliable, whether price is cheap increasingly to be paid close attention to by people.
Such as《In-vitro diagnosis checking system --- it tests oneself with blood glucose monitoring system general technical specifications》(i.e. ISO15197：
2013 standards) in be distinctly claimed, when concentration of glucose is less than 100mg/dl, each packed cell volume level and medium red blood cell pressure
The measured value difference that ponding puts down (42% ± 2%) is no more than 10mg/dl；Concentration of glucose is when more than 100mg/dl, Mei Gehong
The measured value relative difference of cell pack level and medium packed cell volume horizontal (42% ± 2%) is no more than 10%.If test
Whole blood sample containing identical glucose content but with 20%, 42% and 70% packed cell volume value, then by being based on one group of school
The system of normal number (such as slope and intercept of the whole blood sample containing 42% hematocrit value) will report three different Portugals
Grape sugar reading.Even if concentration of glucose is identical, system also contains the whole blood sample for reporting 20% packed cell volume value than 42%
Glucose more than the whole blood sample of packed cell volume value, and the whole blood sample of 70% packed cell volume value contains than 42% red blood cell
The few glucose of the whole blood sample of hematocrit value, this is because the diffusion of red blood cell interference analyte and/or mediator to electrode surface.
Therefore, it is necessary to the concentration of analyte is calibrated according to packed cell volume value.In general, generally use working volts alternating current
Blood sample, acquisition and the relevant electric signal of packed cell volume are tested, passes through dependence test equation and obtains blood sample to be measured
Packed cell volume value, it is final to realize to electrochemistry then using packed cell volume value and the relevant Calibration equation of analyte concentration
The calibration of sensor test analyte.
The operation principle of electrochemical sensor is that the concentration of analyte in test sample is converted to corresponding electric signal, than
Such as when analyte is glucose in blood, the electric current that electrochemical sensor obtains blood sample by applying operating voltage is believed
Number, pass through Calibration equation, you can obtain corresponding blood glucose value.In the electrode process of electrochemical sensor, reactant is first
First to be diffused into electrode surface can just react, and the transmission speed of reactant depends on solution mass tranfer coefficient.Solute in blood
Mass tranfer coefficient is mainly influenced by packed cell volume, and packed cell volume value is higher, and mass tranfer coefficient is smaller, and vice versa.It is in general, high
It will appear minus deviation (analyte concentration calculated is relatively low) under packed cell volume, will appear overgauge under low packed cell volume
(analyte concentration calculated is higher).The packed cell volume range of normal person is about 35% to 55%, however some
For the packed cell volume of people between about 20% to 70%, such broad whole blood hematocrit range can cause electrochemistry
Sensor generates larger error.Accordingly, there exist the reality to the electrochemical sensor with packed cell volume calibration function to need
It asks.
Patent CN104634822A proposes to utilize packed cell volume correction detection concentration of glucose.It wherein refers to that will respond
The peak value of signal is converted into the calculation of packed cell volume value, such as：Y=aX+b, (Y is packed cell volume value, and X is peak value, a
It is predetermined coefficient with b).
Patent CN105021805A proposes a kind of bearing calibration of human body physiological parameter.According to predetermined red blood cell pressure
The correspondence of product value and correction coefficient determines correction coefficient corresponding with the packed cell volume value of the detection sample, utilizes
The correction coefficient is corrected the detectable concentration of the physiological parameter of the detection sample, obtains corrected concentrations, wherein, it is described
Corrected concentrations=correction coefficient * detectable concentrations.
106770461 A of patent CN propose the step of packed cell volume value for obtaining blood sample：In the tertiary voltage
The current value that was obtained between 0-0.5 seconds of application time calculate the first median A1, in the application time of the tertiary voltage
The current value obtained between 1-2 seconds calculates the second median A2, then the packed cell volume value is (A1/A2)*100。
To realize packed cell volume calibration function, the accurate test of realistic existing packed cell volume is first had to.However, and hospital
Environment is different, and domestic consumer is in relatively roomy environment temperature, for example winter is likely to be breached 10 DEG C, and summer is likely to be breached
35℃.The packed cell volume test of blood sample can be by the larger impact of the environment temperature of electrochemical sensor.It therefore must be right
The packed cell volume test of blood sample, which carries out relevant temperature compensation, under different temperatures can just obtain accurate red blood cell pressure
Product value is likely to, eventually by packed cell volume value correcting current value, realize the accurate measurement to analyte.
In addition, the tectonic sieving of electrochemical sensor itself, is also extremely to close for the accurate test for realizing packed cell volume
Important, for example, blood sample introduction is not smooth and correcting electrode system realm can produce testing result by electrolyte pollution etc.
Raw adverse effect.
Invention content
For problems of the prior art, the purpose of the present invention is to provide a kind of red blood cells with temperature-compensating
The electrochemical sensor of hematocrit test function is conducive to electrochemical sensor and is obtained in the use temperature range of bigger accurately
Testing result, and the electrochemical sensor is reasonable in design, simple for process, is conducive to manufacture on a large scale.
For achieving the above object, the present invention adopts the following technical scheme that：
The electrochemical sensor of packed cell volume test function with temperature-compensating, the electrochemical sensor include base
Piece, electrode layer, reaction reagent, pad level and hydrophilic film；Wherein, the injection port end of substrate is equipped with notch；Electrode layer includes correction
Electrode system and detecting electrode system, the two is in the same plane, and correcting electrode system includes a pair of of correcting electrode, by applying
Working volts alternating current is added to carry out packed cell volume test；Detecting electrode system includes a pair of of detecting electrode, by applying direct current work
Make voltage sample introduction is analyzed object test；Pad level includes exhaust pad level, reaction pad level and detection zone, in pad level is reacted
It is provided with detection zone, detection zone narrows from the width setting from inlet.
Further, limitation electrode is equipped between the correcting electrode system and detecting electrode system, limitation electrode is square
Shape carbon electrode.
Further, the shape of the notch is arc；The shape of detection zone is trapezoidal.
Further, the substrate, electrode layer, reaction reagent, pad level and hydrophilic film are set gradually from top to bottom.
Further, the material of the substrate is polyethylene terephthalate；The material of electrode layer is carbon；Pad level
Material is modified polyacrylic acid double faced adhesive tape；The material of hydrophilic film is polyethylene terephthalate.
A kind of temperature-compensation method to packed cell volume test carries out red blood cell pressure using the electrochemical sensor
Product test, when blood sample detects, working volts alternating current is provided for correcting electrode system, red thin with blood sample so as to obtain
The relevant electric signal of born of the same parents' hematocrit, i.e. AD values.Environment temperature T and AD value are substituted into the packed cell volume test side with temperature-compensating
Journey, you can obtain accurate packed cell volume value.Concrete scheme is as follows：
Step 1：Under a certain environment temperature T, the AD values of different packed cell volume blood samples are tested respectively, with red blood cell
Hematocrit value is ordinate, and corresponding A D values are abscissa, carry out fitting of a polynomial, it is preferable that carry out first power linear fit, obtain
(x represents blood sample AD values to packed cell volume measured equation y=ax+b at a temperature of T, and y represents packed cell volume value, and a and b are
Related coefficient).And so on, the packed cell volume measured equation at a temperature of varying environment can be obtained, it is as shown in the table：
Packed cell volume measured equation at a temperature of 1 varying environment of table
Step 2：Packed cell volume measured equation with temperature-compensating is established in the following manner：
Respectively using each temperature as abscissa, corresponding each packed cell volume measured equation Monomial coefficient is ordinate,
Carry out fitting of a polynomial, it is preferable that carry out first power linear fit, obtain z=A1T+B1, (z represents Monomial coefficient, and T is represented
Temperature, A1And B1It is related coefficient)；
Respectively using each temperature as abscissa, corresponding each packed cell volume measured equation constant term is ordinate, is carried out
Fitting of a polynomial, it is preferable that carry out first power linear fit, obtain u=A2T+B2, (u represents constant term, and T represents temperature, A2With
B2It is related coefficient)；
Packed cell volume measured equation with temperature-compensating is y=(A1T+B1)x+(A2T+B2), (y represents red blood cell pressure
Product value, T represent environment temperature, and x represents AD values, A1And B1And A2And B2It is related coefficient)；
Step 3：By the AD values of environment temperature T and blood sample, substituting into step 2 has the packed cell volume of temperature-compensating
Measured equation can obtain accurate packed cell volume value.
The present invention has the advantages that：
(1) there are the notches of arc, reaction pad level on the substrate layer at the injection port end of electrochemical sensor to be provided
The trapezoidal detection zone that inlet narrows from the width, such design improve sample introduction speed so that sample introduction is rapidly completed, and is conducive to
Obtain accurate test value.
(2) the setting limitation electrode between correcting electrode system and detecting electrode system, can effectively prevent detecting electrode system
The electrolyte diffusion in reaction reagent on system is to correcting electrode system, in case correcting electrode system is contaminated influence test value.
(3) between correcting electrode system and detecting electrode system effective district every can individually test：Correcting electrode system
Unified test tries packed cell volume；The various analytes of detecting electrode system testing (analyte can be glucose in blood, uric acid,
Cholesterol etc.), when detecting electrode system testing difference analyte, it may be by electric current of the packed cell volume value to analyte
Value is corrected, so as to obtain more accurate analyte concentration.The design individually tested of electrode system of the present invention, enhances
The scope of application of electrochemical sensor.
(4) electrochemical sensor of the invention is reasonable in design, simple for process, is conducive to manufacture on a large scale.
(5) the present invention also provides a kind of temperature-compensation method to packed cell volume test, be conducive to electrochemical sensing
Device obtains accurate testing result in the use temperature range of bigger.
Description of the drawings
Fig. 1 is the structure diagram of electrochemical sensor of the present invention：Wherein, 1- substrate layers, 11- notches, 2- electrode layers,
The first correcting electrodes of 21-, the second correcting electrodes of 22-, 23- limitation electrodes, the first detecting electrodes of 24-, the second detecting electrodes of 25-,
3- reagent layers, 4- pad levels, 41- exhaust pad levels, 42- reaction pad levels, 43- detection zones, 5- hydrophilic membranes, 6- electrochemistry
Sensor；
Fig. 2 is the contrast schematic diagram for the absolute deviation for having not temperature compensated packed cell volume value under 10 DEG C of environment temperatures；
Fig. 3 is the contrast schematic diagram for the absolute deviation for having not temperature compensated packed cell volume value under 39 DEG C of environment temperatures.
Specific embodiment
Technical solutions according to the invention are further described in detail below by specific embodiment, but it is necessary to
It points out that following embodiment is served only for the description to invention content, does not form limiting the scope of the invention.
As shown in Figure 1, the structure of the electrochemical sensor of the packed cell volume test function with temperature-compensating：From down toward
On successively include substrate layer 1, electrode layer 2, reagent layer 3, pad level 4 and hydrophilic membrane 5；Wherein, the injection port end of substrate layer 1 is stayed
Jagged 11, the notch geometry in the present embodiment is arc.Electrode layer 2 includes correcting electrode system and detecting electrode system, school
Positive electrode system and detecting electrode system are in the same plane, and correcting electrode system includes the first correcting electrode 21 and the second school
Positive electrode 22 carries out packed cell volume test by applying working volts alternating current；Detecting electrode system includes the first detecting electrode
24 and second detecting electrode 25, sample introduction is analyzed by applying direct-current working volts, and object is tested.Reagent is covered in detecting electrode system
Layer 3.Restricted electrode 23 between correcting electrode system and detecting electrode system, the limitation electrode 23 of the present embodiment is rectangle carbon electricity
Pole.Pad level 4 includes exhaust pad level 41, reaction pad level 42 and detection zone 43, and it is padded that detection zone 43 is arranged on reaction
In layer 42, detection zone 43 narrows from the width setting from inlet, it is preferred that the shape of detection zone 43 is trapezoidal.Hydrophilic membrane 5
It is covered on pad level 4.
Preferably, the material of substrate layer 1 is polyethylene terephthalate；The material of electrode layer 2 is carbon；Pad level 4
Material be modified polyacrylic acid double faced adhesive tape, thickness 0.125um；The material of hydrophilic membrane 5 is polyethylene terephthalate, close
Water film 5 passes through single side hydrophilic treated.
The manufacturing process of the electrochemical glucose sensor of the present embodiment is as follows：
The reaction reagent of liquid is completed in allotment first, and reaction reagent then is configured to detection zone by way of liquid
43, (load capacity of every electrochemical sensor is in 0.7-1.2uL).Then in 30 DEG C of -40 DEG C of drying tunnel sections, 40 DEG C of -50 DEG C of drying tunnels
It is heat-treated 10min successively in section, 50 DEG C of -60 DEG C of drying tunnel sections.Double faced adhesive tape is bonded, after cutting, glucose electrochemistry is passed for hydrophilic film
Sensor deposits in the closed plastic barrel with molecular sieve desiccant.Electrochemical glucose sensor can test smallest sample amount
0.6uL。
The experimentation that packed cell volume test is carried out using the electrochemical sensor of the present embodiment is as follows：
First, packed cell volume value is respectively configured and amounts to 6 concentration for 20%, 30%, 42%, 50%, 60% and 70%
The venous whole sample of gradient.Then, respectively at 5 DEG C, at 10 DEG C, at 17 DEG C, at 23 DEG C, at 32 DEG C, 39 DEG C, 45 DEG C of environment
Glucose sensor under temperature condition tests the venous whole sample of above-mentioned 6 concentration gradients successively.During detection, to correction electricity
Electrode systems apply 100hz, and the working volts alternating current of 500mv obtains and the relevant electric signal of packed cell volume, i.e. AD values.For side
Just data processing, by AD values divided by 100000, labeled as test AD values.Under each test condition, retest 5 times is calculated and is surveyed
The average value of AD values is tried, as shown in Table 2-8.
The packed cell volume test data of table 2 at 5 DEG C
Packed cell volume test data of the table 3 at 10 DEG C
Packed cell volume test data of the table 4 at 17 DEG C
Packed cell volume test data of the table 5 at 23 DEG C
Packed cell volume test data of the table 6 at 32 DEG C
Packed cell volume test data of the table 7 at 39 DEG C
Packed cell volume test data of the table 8 at 45 DEG C
It can be seen that AD values have preferable repeatability from table 2-8.
Experimental data in table 2-8 is handled：
(1) respectively at a temperature of 5 DEG C, 10 DEG C, 17 DEG C, 23 DEG C, 32 DEG C, 39 DEG C and 45 DEG C, to test the average value of AD values
For abscissa, corresponding packed cell volume value 20%, 30%, 42%, 50%, 60% and 70% is ordinate, according to least square
Method carries out linear fit, obtains the packed cell volume measured equation of 7 groups of first powers (at a temperature of i.e. not temperature compensated varying environment
Packed cell volume measured equation), as shown in table 9.
(2) with 5 DEG C, 10 DEG C, 17 DEG C, 23 DEG C, 32 DEG C, 39 DEG C and 45 DEG C for abscissa, with red blood cell pressure at each temperature
The Monomial coefficient of product measured equation is ordinate, carries out first power linear fit, obtains z=A1T+B1, wherein, z represents primary
Term coefficient, T represent temperature, A1And B1It is related coefficient.
(3) with 5 DEG C, 10 DEG C, 17 DEG C, 23 DEG C, 32 DEG C, 39 DEG C and 45 DEG C for abscissa, with red blood cell pressure at each temperature
The constant term of product measured equation is ordinate, carries out first power linear fit, obtains u=A2T+B2, wherein, u represents constant term, T
Represent temperature, A2And B2It is related coefficient.
(4) the packed cell volume measured equation with temperature-compensating is：Y=(A1T+B1)x+(A2T+B2), wherein, y represents red
Cell pack value, T represent environment temperature, and x represents AD values, A1And B1And A2And B2It is related coefficient.
By the AD values of environment temperature T and blood sample, y=(A are substituted into1T+B1)x+(A2T+B2), you can it obtains accurately red
Cell pack value.
Packed cell volume measured equation at a temperature of 9 varying environment of table
| Environment temperature (DEG C) | Packed cell volume measured equation | Monomial coefficient | Constant term | R2 |
| 5 | Y=-1.5698x+1.1291 | -1.5698 | 1.1291 | 0.9987 |
| 10 | Y=-1.6449x+1.1767 | -1.6449 | 1.1767 | 0.9994 |
| 17 | Y=-1.431x+1.1391 | -1.4310 | 1.1391 | 0.9965 |
| 23 | Y=-1.2985x+1.1041 | -1.2985 | 1.1041 | 0.9989 |
| 32 | Y=-1.2112x+1.1044 | -1.2112 | 1.1044 | 0.9979 |
| 39 | Y=-1.1087x+1.0774 | -1.1087 | 1.0774 | 0.9830 |
| 45 | Y=-1.0197x+1.0434 | -1.0197 | 1.0434 | 0.9982 |
As can be seen from Table 9, the packed cell volume measured equation at a temperature of varying environment has preferable linear.
Then, using packed cell volume measured equation at a temperature of the varying environment of table 9, respectively using each temperature as horizontal seat
Mark, the Monomial coefficient of corresponding each packed cell volume measured equation is ordinate, and first power linear fit obtains equation (1)：
Z=0.0153T-1.6996 (1)
Wherein, z represents Monomial coefficient, and T represents temperature, R2=0.9538.
Then, using packed cell volume measured equation at a temperature of the varying environment of table 9, respectively using each temperature as horizontal seat
Mark, the constant term coefficient of corresponding each packed cell volume measured equation is ordinate, and first power linear fit obtains equation (2)：
U=-0.0026T+1.1733 (2)
Wherein, u represents constant term coefficient, and T represents temperature, R2=0.7843.
The packed cell volume measured equation with temperature-compensating, i.e. equation (3) are obtained further according to above-mentioned equation (1) and (2)：
Y=(0.0153T-1.6996) x+ (- 0.0026T+1.1733) (3)
Wherein, y represents packed cell volume, and T represents temperature, and x represents AD values.
By the test AD values number of table 2-8 and temperature according to equation (3) is substituted into, the test packed cell volume value of table 10-16 is obtained,
The average value and absolute deviation of five tests are calculated, wherein, absolute deviation=average value-packed cell volume value.
The test packed cell volume value of table 10 at 5 DEG C
Test packed cell volume value of the table 11 at 10 DEG C
Test packed cell volume value of the table 12 at 17 DEG C
Test packed cell volume value of the table 13 at 23 DEG C
Test packed cell volume value of the table 14 at 32 DEG C
Test packed cell volume value of the table 15 at 39 DEG C
Test packed cell volume value of the table 16 at 45 DEG C
From the test packed cell volume value of table 10-16, it can be seen that tested using the packed cell volume with temperature-compensating
Equation, that is, equation (3) tests the error of packed cell volume value within ± 5% in the range of 5 DEG C -45 DEG C, and accuracy is higher, says
The electrochemical sensor of the bright present invention can obtain accurate testing result in larger use temperature range.
Contrast test (whether there is case of temperature compensation)：
(1) using packed cell volume measured equation y=-1.2985x+1.1041 at 23 DEG C in table 9, carry out computing environment temperature
The test packed cell volume for not considering temperature-compensating under 10 DEG C (test AD values are shown in Table 3) and 39 DEG C (test AD values are shown in Table 7)
Value (generally can go to be tested, therefore in test low temperature such as with the packed cell volume equation of the fitting of (23 DEG C ± 2 DEG C) at room temperature
10 DEG C and during such as 39 DEG C of high temperature, deviation is larger, and experimental data is more significant), calculate the average values of five tests and absolutely inclined
Difference, wherein, absolute deviation=average value-packed cell volume value the results are shown in Table shown in 17 and 18.
Test packed cell volume value of the table 17 at 10 DEG C
Test packed cell volume value of the table 18 at 39 DEG C
(2) reuse with temperature-compensating packed cell volume measured equation y=(0.0153T-1.6996) x+ (-
The test of the considerations of 0.0026T+1.1733) (i.e. equation (3)), computing environment temperature is 10 DEG C and 39 DEG C respectively temperature-compensating is red
Cell pack value, and calculate absolute deviation.
(3) as shown in Figures 2 and 3, it can be seen that under 10 DEG C and 39 DEG C of environment, packed cell volume is tested after temperature-compensating
Value is more accurate.
Claims (8)
1. the electrochemical sensor of the packed cell volume test function with temperature-compensating, which is characterized in that the electrochemistry passes
Sensor includes substrate, electrode layer, reaction reagent, pad level and hydrophilic film；Wherein, the injection port end of substrate is equipped with notch；Electrode
Layer includes correcting electrode system and detecting electrode system, and the two is in the same plane, and correcting electrode system includes a pair of correct
Electrode carries out packed cell volume test by applying working volts alternating current；Detecting electrode system includes a pair of of detecting electrode, passes through
Apply the test of direct-current working volts sample introduction is analyzed object；Pad level includes exhaust pad level, reaction pad level and detection zone, anti-
It answers and detection zone is provided in pad level, detection zone narrows from the width setting from inlet.
2. the electrochemical sensor of the packed cell volume test function according to claim 1 with temperature-compensating, special
Sign is, limitation electrode is equipped between the correcting electrode system and detecting electrode system, and limitation electrode is rectangle carbon electrode.
3. the electrochemical sensor of the packed cell volume test function according to claim 1 with temperature-compensating, special
Sign is that the shape of the notch is arc；The shape of detection zone is trapezoidal.
4. the electrochemistry biography of the packed cell volume test function with temperature-compensating according to claim 1-3 is one of arbitrary
Sensor, which is characterized in that the substrate, electrode layer, reaction reagent, pad level and hydrophilic film are set gradually from top to bottom.
5. the electrochemistry biography of the packed cell volume test function with temperature-compensating according to claim 1-3 is one of arbitrary
Sensor, which is characterized in that the material of the substrate is polyethylene terephthalate；The material of electrode layer is carbon；Pad level
Material is modified polyacrylic acid double faced adhesive tape；The material of hydrophilic film is polyethylene terephthalate.
6. a kind of temperature-compensation method to packed cell volume test, which is characterized in that utilize electrochemistry described in claim 1
Sensor carries out packed cell volume test, and the correcting electrode system provides working volts alternating current, so as to obtain and blood sample
The relevant electric signal of packed cell volume, i.e. AD values；And the packed cell volume measured equation with temperature-compensating is established, by environment temperature
It spends T and AD values and substitutes into the packed cell volume measured equation with temperature-compensating, you can obtain accurate packed cell volume value.
7. the according to the method described in claim 6, it is characterized in that, packed cell volume measured equation with temperature-compensating
It is to establish in the following manner：
(1) under a certain environment temperature T, the AD values of different packed cell volume blood samples are tested respectively, with packed cell volume value
For ordinate, corresponding A D values are abscissa, carry out fitting of a polynomial, obtain the packed cell volume measured equation y under environment temperature T
=ax+b, wherein, x represents AD values, and y represents packed cell volume value, and a and b are related coefficients, and so on, difference can be obtained
Packed cell volume measured equation under environment temperature；
(2) respectively using each temperature as abscissa, the Monomial coefficient of corresponding each packed cell volume measured equation is ordinate,
Fitting of a polynomial is carried out, obtains z=A1T+B1, wherein, z represents Monomial coefficient, and T represents temperature, A1And B1It is related coefficient；
(3) respectively using each temperature as abscissa, the constant term of corresponding each packed cell volume measured equation is ordinate, is carried out
Fitting of a polynomial obtains u=A2T+B2, wherein, u represents constant term, and T represents temperature, A2And B2It is related coefficient；
(4) the packed cell volume measured equation with temperature-compensating is obtained：Y=(A1T+B1)x+(A2T+B2), wherein, y represents red
Cell pack value, T represent environment temperature, and x represents AD values, A1And B1And A2And B2It is related coefficient.
8. the method according to the description of claim 7 is characterized in that the fitting of a polynomial is first power linear fit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711366439.6A CN108195900B (en) | 2017-12-18 | 2017-12-18 | Electrochemical sensor with temperature compensated hematocrit test function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711366439.6A CN108195900B (en) | 2017-12-18 | 2017-12-18 | Electrochemical sensor with temperature compensated hematocrit test function |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108195900A true CN108195900A (en) | 2018-06-22 |
| CN108195900B CN108195900B (en) | 2024-01-05 |
Family
ID=62574484
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711366439.6A Active CN108195900B (en) | 2017-12-18 | 2017-12-18 | Electrochemical sensor with temperature compensated hematocrit test function |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108195900B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108896635A (en) * | 2018-09-20 | 2018-11-27 | 江苏鱼跃医疗设备股份有限公司 | A kind of beta-hydroxybutyric acid electrochemical sensor |
| CN108982501A (en) * | 2018-07-25 | 2018-12-11 | 赖海燕 | A kind of medical treatment blood detector equipment and system |
| CN109341899A (en) * | 2018-09-21 | 2019-02-15 | 深圳市国赛生物技术有限公司 | Temperature correction method, device and computer readable storage medium |
| CN109884150A (en) * | 2019-03-08 | 2019-06-14 | 武汉璟泓万方堂医药科技股份有限公司 | A kind of red blood cell overstocks bearing calibration and storage medium |
| CN111239228A (en) * | 2020-02-24 | 2020-06-05 | 江苏鱼跃医疗设备股份有限公司 | Electrochemical biosensor and method for measuring blood impedance phase angle |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999030144A1 (en) * | 1997-12-11 | 1999-06-17 | The Victoria University Of Manchester | Sensor devices and analytical method |
| WO2002054055A1 (en) * | 2001-01-04 | 2002-07-11 | Tyson Bioresearch, Inc. | Biosensors having improved sample application |
| US20030082076A1 (en) * | 2000-09-01 | 2003-05-01 | Yueh-Hui Lin | Disposable electrode for whole blood hemoglobin (HGB) and hematocrit (HCT) measurement, and preparation and application |
| US20060231423A1 (en) * | 2005-04-15 | 2006-10-19 | Agamatrix, Inc | Analyte determination method and analyte meter |
| US20080149480A1 (en) * | 2006-12-22 | 2008-06-26 | Home Diagnostics, Inc. | Gel formation to reduce hematocrit sensitivity in electrochemical test |
| JP2009097877A (en) * | 2007-10-12 | 2009-05-07 | National Institute Of Advanced Industrial & Technology | Biosensor chip |
| US20090184004A1 (en) * | 2008-01-17 | 2009-07-23 | Lifescan, Inc. | System and method for measuring an analyte in a sample |
| WO2011030093A1 (en) * | 2009-09-04 | 2011-03-17 | Lifescan Scotland Limited | Glucose measurement method and system |
| US20140178909A1 (en) * | 2012-12-21 | 2014-06-26 | Abbott Diabetes Care Inc. | Method for Improving Measurement Accuracy and Devices and Systems Related Thereto |
| US20150160150A1 (en) * | 2013-12-06 | 2015-06-11 | Changsha Sinocare Inc. | Disposable test sensor with improved sampling entrance |
| US20150198554A1 (en) * | 2014-01-13 | 2015-07-16 | Changsha Sinocare Inc. | Disposable test sensor |
| GB201510765D0 (en) * | 2015-06-18 | 2015-08-05 | Inside Biometrics Ltd | Method, apparatus and electrochemical test device |
| CN106596688A (en) * | 2016-12-21 | 2017-04-26 | 三诺生物传感股份有限公司 | Method, recognition device controller and recognition system for distinguishing quality control liquid from practical sample in electrochemical testing system |
| CN106770573A (en) * | 2017-01-05 | 2017-05-31 | 江苏鱼跃医疗设备股份有限公司 | A kind of glucose sensor |
| CN207557163U (en) * | 2017-12-18 | 2018-06-29 | 江苏鱼跃医疗设备股份有限公司 | The electrochemical sensor of packed cell volume test function with temperature-compensating |
-
2017
- 2017-12-18 CN CN201711366439.6A patent/CN108195900B/en active Active
Patent Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999030144A1 (en) * | 1997-12-11 | 1999-06-17 | The Victoria University Of Manchester | Sensor devices and analytical method |
| US20030082076A1 (en) * | 2000-09-01 | 2003-05-01 | Yueh-Hui Lin | Disposable electrode for whole blood hemoglobin (HGB) and hematocrit (HCT) measurement, and preparation and application |
| WO2002054055A1 (en) * | 2001-01-04 | 2002-07-11 | Tyson Bioresearch, Inc. | Biosensors having improved sample application |
| US20040031698A1 (en) * | 2001-01-04 | 2004-02-19 | Lee Jin Po | Biosensors having improved sample application and measuring properties and uses thereof |
| US20060231423A1 (en) * | 2005-04-15 | 2006-10-19 | Agamatrix, Inc | Analyte determination method and analyte meter |
| US20080149480A1 (en) * | 2006-12-22 | 2008-06-26 | Home Diagnostics, Inc. | Gel formation to reduce hematocrit sensitivity in electrochemical test |
| JP2009097877A (en) * | 2007-10-12 | 2009-05-07 | National Institute Of Advanced Industrial & Technology | Biosensor chip |
| US20090184004A1 (en) * | 2008-01-17 | 2009-07-23 | Lifescan, Inc. | System and method for measuring an analyte in a sample |
| WO2011030093A1 (en) * | 2009-09-04 | 2011-03-17 | Lifescan Scotland Limited | Glucose measurement method and system |
| US20140178909A1 (en) * | 2012-12-21 | 2014-06-26 | Abbott Diabetes Care Inc. | Method for Improving Measurement Accuracy and Devices and Systems Related Thereto |
| US20150160150A1 (en) * | 2013-12-06 | 2015-06-11 | Changsha Sinocare Inc. | Disposable test sensor with improved sampling entrance |
| US20150198554A1 (en) * | 2014-01-13 | 2015-07-16 | Changsha Sinocare Inc. | Disposable test sensor |
| GB201510765D0 (en) * | 2015-06-18 | 2015-08-05 | Inside Biometrics Ltd | Method, apparatus and electrochemical test device |
| CN106596688A (en) * | 2016-12-21 | 2017-04-26 | 三诺生物传感股份有限公司 | Method, recognition device controller and recognition system for distinguishing quality control liquid from practical sample in electrochemical testing system |
| CN106770573A (en) * | 2017-01-05 | 2017-05-31 | 江苏鱼跃医疗设备股份有限公司 | A kind of glucose sensor |
| CN207557163U (en) * | 2017-12-18 | 2018-06-29 | 江苏鱼跃医疗设备股份有限公司 | The electrochemical sensor of packed cell volume test function with temperature-compensating |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108982501A (en) * | 2018-07-25 | 2018-12-11 | 赖海燕 | A kind of medical treatment blood detector equipment and system |
| CN108896635A (en) * | 2018-09-20 | 2018-11-27 | 江苏鱼跃医疗设备股份有限公司 | A kind of beta-hydroxybutyric acid electrochemical sensor |
| CN109341899A (en) * | 2018-09-21 | 2019-02-15 | 深圳市国赛生物技术有限公司 | Temperature correction method, device and computer readable storage medium |
| CN109341899B (en) * | 2018-09-21 | 2021-02-09 | 深圳市国赛生物技术有限公司 | Temperature correction method, device and computer readable storage medium |
| CN109884150A (en) * | 2019-03-08 | 2019-06-14 | 武汉璟泓万方堂医药科技股份有限公司 | A kind of red blood cell overstocks bearing calibration and storage medium |
| CN109884150B (en) * | 2019-03-08 | 2021-05-14 | 武汉璟泓科技股份有限公司 | Erythrocyte backlog correction method and storage medium |
| CN111239228A (en) * | 2020-02-24 | 2020-06-05 | 江苏鱼跃医疗设备股份有限公司 | Electrochemical biosensor and method for measuring blood impedance phase angle |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108195900B (en) | 2024-01-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108195900A (en) | The electrochemical sensor of packed cell volume test function with temperature-compensating | |
| CN207557163U (en) | The electrochemical sensor of packed cell volume test function with temperature-compensating | |
| CN102778487B (en) | Oxidizable species as an internal reference in control solutions for biosensors | |
| JP6522067B2 (en) | System, apparatus and method for improving biosensor accuracy using fill time | |
| TWI547687B (en) | Calibration method for blood glucose of blood sample and calibration system of the same | |
| EP0928967B1 (en) | Method and apparatus for the determination of a substance coexisting with another substance | |
| US3707455A (en) | Measuring system | |
| Rhodes et al. | Prediction of pocket-portable and implantable glucose enzyme electrode performance from combined species permeability and digital simulation analysis | |
| US7771583B2 (en) | Electrochemical determination of analytes | |
| AU2010249255B2 (en) | Fill sufficiency method and system | |
| CN101675338A (en) | System and method for analyte measurement using a nonlinear sample response | |
| CN103884762B (en) | Method for detecting concentration and diffusion factor of substance to be detected in sample and test piece | |
| CN103328962A (en) | Systems and methods for improved stability of electrochemical sensors | |
| KR101357134B1 (en) | Method for Measuring Analytes in Blood Samples Using Electrochemical Biosensor and a Portable Analyzer | |
| Wang et al. | All-solid-state blood calcium sensors based on screen-printed poly (3, 4-ethylenedioxythiophene) as the solid contact | |
| CN105784814A (en) | Sensor based on concentration cell principle | |
| CN105203613B (en) | The bearing calibration of the blood glucose value of blood sample | |
| CN108896635A (en) | A kind of beta-hydroxybutyric acid electrochemical sensor | |
| Fu et al. | Electrochemical test strip-based accurate blood uric acid measurement by adding blood cell filtration membrane | |
| CN111896759B (en) | Method, device and storage medium for eliminating influence of sample injection process | |
| CN208795691U (en) | A kind of beta-hydroxybutyric acid electrochemical sensor | |
| CN110243914A (en) | A kind of all solid state electrochemistry macromolecule sensor measuring dissolved oxygen | |
| TW201719162A (en) | Detection method for detecting blood glucose and hemoglobin of blood sample | |
| TWI531789B (en) | Calibration method for blood glucose of blood sample | |
| JP5001220B2 (en) | Substrate concentration measuring apparatus, method and program |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20220114 Address after: 212300 No. 5 Baisheng Road, Danyang Development Zone, Zhenjiang City, Jiangsu Province Applicant after: Jiangsu Yuyue kailite Biotechnology Co.,Ltd. Address before: 212300 Yunyang Industrial Park, Danyang City, Zhenjiang City, Jiangsu Province Applicant before: JIANGSU YUYUE MEDICAL EQUIPMENT&SUPPLY Co.,Ltd. Applicant before: NANJING YUWELL SOFTWARE TECHNOLOGY Co.,Ltd. Applicant before: JIANGSU YUWELL TECHNOLOGY DEVELOPMENT CO.,LTD. Applicant before: JIANGSU YUYUE INFORMATION TECHNOLOGY SYSTEM Co.,Ltd. Applicant before: SUZHOU YUYUE MEDICAL TECHNOLOGY Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |