CN108892563A - A kind of production selenium promotor and preparation method thereof for improving chlorella and producing organic selenium - Google Patents
A kind of production selenium promotor and preparation method thereof for improving chlorella and producing organic selenium Download PDFInfo
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- CN108892563A CN108892563A CN201810836645.7A CN201810836645A CN108892563A CN 108892563 A CN108892563 A CN 108892563A CN 201810836645 A CN201810836645 A CN 201810836645A CN 108892563 A CN108892563 A CN 108892563A
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Abstract
The present invention provides the production selenium promotors that a kind of raising chlorella produces organic selenium, including following parts by weight material：Micro-nano biogenic opal and dry powder adjust agent composition 1-10 parts, 0.002-3 parts of Quercetin, 0.005-5 parts of sodium selenite, 982-998.993 parts of polyethylene glycol；It is (1-2) that the micro-nano biogenic opal and dry powder adjusting agent composition, which include weight part ratio,:The nano silica and Chinese pistache extract dry powder of (4-6).The preparation method provided by the invention for producing selenium promotor opens the industry culture new way of microalgae high yield organic selenium, and materials are simple, operation is convenient, at low cost；Product Green used, environmental protection, safety, plant origin Flavonoid substances can be used as a kind of natural plants stimulin, promote the photosynthetic metabolite reaction of frustule, improve the accumulation of the growth potential and chlorella cells internal carbon source substrate of chlorella, to improve its growth rate, solves the problems, such as the industrialization culture of microalgae fast-growth.
Description
Technical field
The present invention relates to biomass as resources and field of biological energy source more particularly to a kind of promotion chlorella quickly produce it is organic
Promotor of selenium and preparation method thereof.
Background technique
The growth and development of humans and animals and plant be unable to do without microelement, and selenium is then important micro member needed by human
One of element provides indispensable nutrient for the maintenance of its vital movement.Chlorella, can be by the inorganic of absorption in microalgae
Selenium be effectively converted into the form of seleno nucleic acid, selenoprotein, selenium polysaccharide etc. existing for organic selenium and simple substance sodium selenite particle.
The organic selenium toxicity of plant source is low, and function can be used not only to improve human body oxidation resistance, reduces unsaturated acids in cell
Oxidation reaction occur, can also pass through participate in immune response, improve B cell and T cell proliferation activity, prevent body metabolism
Toxic metabolite is generated, provides advantageous anti-oxidant barrier for the metabolism of mankind's normal physiological.
The Flavonoid substances Quercetin of plant source contains multiple phenolic hydroxyl groups, verifies it by certain external model of oxidation
Have the function of free radical activity and antibacterial, pharmacological activity antiviral, anticancer is antitumor in anti-oxidant scavenger-cell.Big portion
The Flavonoid substances of point plant source can be used as a kind of natural plants stimulin and cause stress pressure to frustule, to influence microalgae
The biochemical metabolism process of cell interior organic selenium.
Chlorella throughput rate is higher than many terrestrial plants, is easy to large-scale culture, and economic value is high, can be used for producing life
Object diesel oil, protein and secondary metabolites carotenoid, astaxanthin, especially unsaturated fatty acid etc..It can be held as green
The bioenergy of supervention exhibition, needs to develop the industrial condition of convenient low cost to improve production organic selenium potential and the life of chlorella
Object amount.Research is found after giving bead algae culture medium addition exogenous biological stimulin, can cause stress pressure to improve to it
It produces organic selenium potentiality, and efficient productivity, which is offset to a certain extent necessary to cultivation culture chlorella photosynthetic response, to be had
Machine carbon source cost is subsequent High Density Cultivation chlorella, and the exploitation for obtaining a series of metabolites of high added value provides research
Basis.
Therefore, it is badly in need of developing a kind of training of efficient, environmental protection chlorella production organic selenium in chlorella industrialized production at present
The method of supporting.
Summary of the invention
Technical problem to be solved by the present invention lies in provide a kind of production selenium promotor of raising chlorella production organic selenium.
The present invention solves above-mentioned technical problem using following technical scheme：
A kind of production selenium promotor for improving chlorella and producing organic selenium, including following parts by weight material：Micro-nano biogenic opal and dry
Powder adjusts agent composition 1-10 parts, 0.002-3 parts of Quercetin, 0.005-5 parts of sodium selenite, 982-998.993 parts of polyethylene glycol；
It is (1-2) that the micro-nano biogenic opal and dry powder adjusting agent composition, which include weight part ratio,:The nano silica of (4-6) and the coptis
The wooden extract dry powder.
Further, the step of preparation method of the micro-nano biology Si powder includes：Using oxygen and ammonia as gas source,
SiO 2 powder is carried out under vacuum conditions using the plasma that temperature is 300-800 DEG C, power is 1-3KW intermittent
Radiation treatment, and kept for total effective irradiation time up to 20-60 minutes, micro-nano biology Si powder is made.
Further, the volume ratio of the oxygen and ammonia is (1-2):(4-6).
Further, the intermittent radiation treatment mode is：Radiation treatment 5-7 minutes, stop 5-15 minutes.
Further, the SiO 2 powder is the micron-sized SiO 2 powder of 150-300.
Further, the step of preparation method of the Chinese pistache extract dry powder includes：By the Chinese pistache blade of acquisition
It is placed in 100-140 DEG C of progress in baking oven and collects blade after being sterilized, pulverize and sieve after drying, then it is heated and carries out 70-90
, to being filtered under diminished pressure again after broken mixture constant volume, then 10- is centrifuged to its 4500-5500rpm DEG C ultrasonic wave extraction 1-3 time
30min, while with membrane filtration degerming, retain supernatant and is freeze-dried up to Chinese pistache extract dry powder.
Further, including following parts by weight material：Micro-nano biogenic opal and dry powder adjust 5 parts of agent composition, Quercetin
0.005 part, 0.14 part of sodium selenite, 994.855 parts of polyethylene glycol；The micro-nano biogenic opal and dry powder adjust agent composition
Weight part ratio is 1.5:5 nano silica and Chinese pistache extract dry powder.
Further, including following parts by weight material：Micro-nano biogenic opal and dry powder adjust 1 part of agent composition, Quercetin
0.015 part, 0.42 part of sodium selenite, 998.565 parts of polyethylene glycol；The micro-nano biogenic opal and dry powder adjust agent composition
Weight part ratio is 2:4 nano silica and Chinese pistache extract dry powder.
This preparation method for improving chlorella and producing the production selenium promotor of organic selenium, step include：
(1) micro-nano biology Si powder and Chinese pistache extract dry powder are prepared respectively, are added in Chinese pistache extract dry powder
Nano grade silica particles obtain micro-nano biogenic opal and dry powder adjusts agent composition；
(2) micro-nano biogenic opal and dry powder adjusting agent composition, sodium selenite, Quercetin and polyethylene glycol are fullyd shake mixed
It is even, chlorella is made and produces selenium promotor.
Further, blending manner fullys shake in the step (2) is：30-90 is handled under 4000-9000rpm revolving speed
Minute.
The advantage of the invention is that：
(1) micro-nano biogenic opal/dry powder, sodium selenite, plant source biostimulant, polyethylene glycol are utilized, chlorella is promoted
Produce organic selenium.A kind of plant source nanocomposite is prepared, the Flavonoid substances of plant source can be used as a kind of natural plants thorn
Hormone, regulation frustule produce organic selenium metabolic process, improve its antioxygenic property and produce organic selenium potential, to influence frustule
Biomass Accumulation；
(2) preparation method is simple, easy to operate, low in cost, can carry out large-scale production.
(3) product is natural, green, environmentally friendly, safe, without side-effects, and chlorella can be made to produce organic selenium conversion ratio and improved
1.6 times or so, effectively improve the photosynthesis organic selenium transformation efficiency of chlorella and its potential of industrial applications.
Detailed description of the invention
Fig. 1 is that chlorella produces selenium promotor to chlorella production selenium conversion ratio influence in 1-4 of the embodiment of the present invention and control group
Effect contrast figure.
Specific embodiment
The present invention will be further described With reference to embodiment, following embodiment be intended to illustrate invention without
It is limitation of the invention further.
Embodiment 1
A kind of production selenium promotor for improving chlorella and producing organic selenium, the raw material including following parts by weight：Micro-nano biogenic opal
5 parts of agent composition, 0.005 part of Quercetin, 0.14 part of sodium selenite, 994.855 parts of polyethylene glycol are adjusted with dry powder；Wherein, micro-
Receiving biogenic opal and dry powder adjusts agent composition by nano silica and Chinese pistache extract dry powder according to mass ratio 1.5:5 mixing
It forms.
This preparation method for improving chlorella and producing the production selenium promotor of organic selenium, step include：
(1) with volume ratio for 1:4 oxygen and ammonia are gas source, utilize the plasma that temperature is 400 DEG C, power is 1KW
Body carries out intermittent radiation treatment, i.e. radiation treatment 5 minutes to 200 micron-sized silica under vacuum conditions, stops 10 points
Clock is kept for total effective irradiation time up to 40 minutes, and micro-nano biology Si powder is made；
(2) the Chinese pistache blade of acquisition is placed in baking oven after 120 DEG C of progress is sterilized and collects blade, crushed after drying
Sieving, then it is heated 80 DEG C of progress ultrasonic wave extraction 2 times, to being filtered under diminished pressure again after broken mixture constant volume, then to it
5000rpm be centrifuged 20min, while use membrane filtration degerming, retain supernatant be freeze-dried to obtain the final product Chinese pistache extract dry powder.Again plus
Enter nano grade silica particles to be mixed according to above-mentioned mass ratio, obtains micro-nano biogenic opal and dry powder adjusts agent composition；
(3) the micro-nano biogenic opal and dry powder of above-mentioned parts by weight are adjusted into agent composition, sodium selenite, Quercetin and poly- second
Mixing fullys shake in glycol, handles 80 minutes under 5000rpm revolving speed, and chlorella is made and produces selenium promotor.
Statistics indicate that the processing group for producing selenium promotor is added compared with blank control, the 7th day production organic selenium to chlorella
Conversion ratio can reach 1.43 times of common process group.
Embodiment 2
A kind of production selenium promotor for improving chlorella and producing organic selenium, the raw material including following parts by weight：Micro-nano biogenic opal
1 part of agent composition, 0.015 part of Quercetin, 0.42 part of sodium selenite, 998.565 parts of polyethylene glycol is adjusted with dry powder；Wherein, micro-
Receiving biogenic opal and dry powder adjusts agent composition by nano silica and Chinese pistache extract dry powder according to mass ratio 2:4 mixing and
At.
This preparation method for improving chlorella and producing the production selenium promotor of organic selenium, step include：
(1) with volume ratio for 1:4 oxygen and ammonia are gas source, utilize the plasma that temperature is 400 DEG C, power is 1KW
Body carries out intermittent radiation treatment, i.e. radiation treatment 5 minutes to 200 micron-sized silica under vacuum conditions, stops 10 points
Clock is kept for total effective irradiation time up to 40 minutes, and micro-nano biology Si powder is made；
(2) the Chinese pistache blade of acquisition is placed in baking oven after 100 DEG C of progress is sterilized and collects blade, crushed after drying
Sieving, then it is heated 80 DEG C of progress ultrasonic wave extraction 2 times, to being filtered under diminished pressure again after broken mixture constant volume, then to it
4500rpm be centrifuged 30min, while use membrane filtration degerming, retain supernatant be freeze-dried to obtain the final product Chinese pistache extract dry powder.Again plus
Enter nano grade silica particles to be mixed according to above-mentioned mass ratio, obtains micro-nano biogenic opal and dry powder adjusts agent composition；
(3) the micro-nano biogenic opal and dry powder of above-mentioned parts by weight are adjusted into agent composition, sodium selenite, Quercetin and poly- second
Mixing fullys shake in glycol, handles 80 minutes under 5000rpm revolving speed, and chlorella is made and produces selenium promotor.
Statistics indicate that the processing group for producing selenium promotor is added compared with blank control, the 7th day production organic selenium to chlorella
Conversion ratio can reach 1.62 times of common process group.
Embodiment 3
A kind of production selenium promotor for improving chlorella and producing organic selenium, the raw material including following parts by weight：Micro-nano biogenic opal
3 parts of agent composition, 0.035 part of Quercetin, 0.70 part of sodium selenite, 996.265 parts of polyethylene glycol are adjusted with dry powder；Wherein, micro-
Receiving biogenic opal and dry powder adjusts agent composition by nano silica and Chinese pistache extract dry powder according to mass ratio 2.5:5.5 mixed
It closes.
This preparation method for improving chlorella and producing the production selenium promotor of organic selenium, step include：
(1) with volume ratio for 2:6 oxygen and ammonia are gas source, utilize the plasma that temperature is 800 DEG C, power is 3KW
Intermittent radiation treatment, i.e. radiation treatment 6 minutes are carried out to silica (300 micron order) under vacuum conditions, stopped 10 minutes,
It is kept for total effective irradiation time up to 60 minutes, micro-nano biology Si powder is made；
(2) the Chinese pistache blade of acquisition is placed in baking oven after 120 DEG C of progress is sterilized and collects blade, crushed after drying
Sieving, then it is heated 60 DEG C of progress ultrasonic wave extraction 2 times, to being filtered under diminished pressure again after broken mixture constant volume, then to it
8000rpm be centrifuged 10min, while use membrane filtration degerming, retain supernatant be freeze-dried to obtain the final product Chinese pistache extract dry powder.Again plus
Enter nano grade silica particles to be mixed according to above-mentioned mass ratio, obtains micro-nano biogenic opal and dry powder adjusts agent composition；
(3) the micro-nano biogenic opal and dry powder of above-mentioned parts by weight are adjusted into agent composition, sodium selenite, Quercetin and poly- second
Mixing fullys shake in glycol, handles 60 minutes under 6000rpm revolving speed, and chlorella is made and produces selenium promotor.
Statistics indicate that the processing group for producing selenium promotor is added compared with blank control, the 7th day production organic selenium to chlorella
Conversion ratio can reach 1.65 times of common process group.
Embodiment 4
A kind of production selenium promotor for improving chlorella and producing organic selenium, the raw material including following parts by weight：Micro-nano biogenic opal
9 parts of agent composition, 0.02 part of Quercetin, 0.98 part of sodium selenite, 990 parts of polyethylene glycol are adjusted with dry powder；Wherein, micro-nano biology
Silicon and dry powder adjust agent composition by nano silica and Chinese pistache extract dry powder according to mass ratio 1:6 mix.
This preparation method for improving chlorella and producing the production selenium promotor of organic selenium, step include：
(1) with volume ratio for 2:6 oxygen and ammonia are gas source, utilize the plasma that temperature is 800 DEG C, power is 3KW
Intermittent radiation treatment, i.e. radiation treatment 6 minutes are carried out to silica (300 micron order) under vacuum conditions, stopped 10 minutes,
It is kept for total effective irradiation time up to 60 minutes, micro-nano biology Si powder is made；
(2) the Chinese pistache blade of acquisition is placed in baking oven after 120 DEG C of progress is sterilized and collects blade, crushed after drying
Sieving, then it is heated 60 DEG C of progress ultrasonic wave extraction 2 times, to being filtered under diminished pressure again after broken mixture constant volume, then to it
8000rpm be centrifuged 10min, while use membrane filtration degerming, retain supernatant be freeze-dried to obtain the final product Chinese pistache extract dry powder.Again plus
Enter nano grade silica particles to be mixed according to above-mentioned mass ratio, obtains micro-nano biogenic opal and dry powder adjusts agent composition；
(3) the micro-nano biogenic opal and dry powder of above-mentioned parts by weight are adjusted into agent composition, sodium selenite, Quercetin and poly- second
Mixing fullys shake in glycol, handles 60 minutes under 6000rpm revolving speed, and chlorella is made and produces selenium promotor.
Statistics indicate that the processing group for producing selenium promotor is added compared with blank control, the 7th day production organic selenium to chlorella
Conversion ratio can reach 1.58 times of common process group.
Selenium promotor is produced Fig. 1 shows chlorella in embodiment 1-4 and control group, and the effect that selenium conversion ratio influences is produced on chlorella
Fruit comparison diagram, control group is not use control group corresponding to the processing group frustule of the production selenium promotor in figure, and processing group is
Using the experimental group of the chlorella growth promotor of embodiment 1-4 preparation；
The result shows that：The chlorella being prepared in embodiment, which produces selenium promotor, can effectively improve chlorella production organic selenium
Transformation efficiency.
Micro-nano biogenic opal and dry powder adjust agent composition, sodium selenite, Quercetin, polyethylene glycol in the present embodiment, promote
Chlorella organic selenium conversion ratio.In culture medium needed for this chlorella production selenium promotor is added to its growth, pass through promotion
The photosynthetic metabolite of frustule reacts, and improves the accumulation of the growth potential and chlorella cells internal carbon source substrate of chlorella, from
And it improves it and produces selenium transformation efficiency.
Although illustrate and describing the present invention with specific embodiment, the foregoing is merely the preferable realities of the invention
Example is applied, is not intended to limit the invention creation, all any modifications made within the spirit and principle of the invention,
Equivalent replacement and improvement etc., should be included within the protection scope of the invention.
Claims (10)
1. a kind of production selenium promotor for improving chlorella and producing organic selenium, which is characterized in that including following parts by weight material：Micro-nano is raw
Agent composition 1-10 parts of object silicon and dry powder adjusting, 0.002-3 parts of Quercetin, 0.005-5 parts of sodium selenite, polyethylene glycol 982-
998.993 part；It is (1-2) that the micro-nano biogenic opal and dry powder adjusting agent composition, which include weight part ratio,:The micro-nano biology of (4-6)
Si powder and Chinese pistache extract dry powder.
2. a kind of production selenium promotor for improving chlorella and producing organic selenium according to claim 1, which is characterized in that described micro-
Receive biological Si powder preparation method the step of include：It is 300-800 DEG C, function using temperature using oxygen and ammonia as gas source
The plasma that rate is 1-3KW carries out intermittent radiation treatment to SiO 2 powder under vacuum conditions, and keeps total effective
Irradiation time reaches 20-60 minutes, and micro-nano biology Si powder is made.
3. a kind of preparation method for improving chlorella and producing the production selenium promotor of organic selenium according to claim 2, feature
It is, the volume ratio of the oxygen and ammonia is (1-2):(4-6).
4. a kind of preparation method for improving chlorella and producing the production selenium promotor of organic selenium according to claim 2, feature
It is, the intermittence radiation treatment mode is：Radiation treatment 5-7 minutes, stop 5-15 minutes.
5. a kind of preparation method for improving chlorella and producing the production selenium promotor of organic selenium according to claim 2, feature
It is, the SiO 2 powder is the micron-sized SiO 2 powder of 150-300.
6. a kind of production selenium promotor for improving chlorella and producing organic selenium according to claim 1, which is characterized in that the Huang
Even the step of preparation method of the wooden extract dry powder includes：The Chinese pistache blade of acquisition is placed in baking oven and carries out 100-140 DEG C
Collect blade after being sterilized, pulverize and sieve after drying, then it is heated progress 70-90 DEG C ultrasonic wave extraction 1-3 time, to be crushed
It is filtered under diminished pressure again after mixture constant volume afterwards, then 10-30min is centrifuged to its 4500-5500rpm, while with membrane filtration degerming,
Retain supernatant to be freeze-dried up to Chinese pistache extract dry powder.
7. it is according to claim 1 it is a kind of improve chlorella produce organic selenium production selenium promotor, which is characterized in that including with
Lower parts by weight material：Micro-nano biogenic opal and dry powder adjust 5 parts of agent composition, 0.005 part of Quercetin, 0.14 part of sodium selenite, gather
994.855 parts of ethylene glycol；It is 1.5 that the micro-nano biogenic opal and dry powder adjusting agent composition, which include weight part ratio,:5 nano-silica
SiClx and Chinese pistache extract dry powder.
8. it is according to claim 1 it is a kind of improve chlorella produce organic selenium production selenium promotor, which is characterized in that including with
Lower parts by weight material：Micro-nano biogenic opal and dry powder adjust 1 part of agent composition, 0.015 part of Quercetin, 0.42 part of sodium selenite, gather
998.565 parts of ethylene glycol；It is 2 that the micro-nano biogenic opal and dry powder adjusting agent composition, which include weight part ratio,:4 nanometer titanium dioxide
Silicon and Chinese pistache extract dry powder.
9. a kind of prepare such as a kind of described in any item sides improved chlorella and produce the production selenium promotor of organic selenium claim 1-8
Method, which is characterized in that step includes：
(1) micro-nano biology Si powder and Chinese pistache extract dry powder are prepared respectively, and nanometer is added in Chinese pistache extract dry powder
SiO 2 powder obtains micro-nano biogenic opal and dry powder adjusts agent composition；
(2) micro-nano biogenic opal and dry powder are adjusted into agent composition, sodium selenite, Quercetin and polyethylene glycol and mixing fully shake,
Chlorella is made and produces selenium promotor.
10. a kind of preparation method for improving chlorella and producing the production selenium promotor of organic selenium according to claim 9, feature
It is, blending manner, which fullys shake, in the step (2) is：It is handled 30-90 minutes under 4000-9000rpm revolving speed.
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| US20160340264A1 (en) * | 2012-02-13 | 2016-11-24 | Heliae Development Llc | Microalgae as a mineral vehicle in aquafeeds |
| CN107347541A (en) * | 2017-08-30 | 2017-11-17 | 武汉五谷天下生态农业有限公司 | A kind of cultivation implantation methods of selenium-enriched rice |
| CN108004195A (en) * | 2017-12-29 | 2018-05-08 | 中国科学院合肥物质科学研究院 | A kind of method for promoting rapid growth of chlorella using Chinese sumac leaf extract |
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2018
- 2018-07-26 CN CN201810836645.7A patent/CN108892563A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20160340264A1 (en) * | 2012-02-13 | 2016-11-24 | Heliae Development Llc | Microalgae as a mineral vehicle in aquafeeds |
| CN107347541A (en) * | 2017-08-30 | 2017-11-17 | 武汉五谷天下生态农业有限公司 | A kind of cultivation implantation methods of selenium-enriched rice |
| CN108004195A (en) * | 2017-12-29 | 2018-05-08 | 中国科学院合肥物质科学研究院 | A kind of method for promoting rapid growth of chlorella using Chinese sumac leaf extract |
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Application publication date: 20181127 |