CN108850544A - A kind of plantation of safe utilization is in the method for the fibre ramie by-product of cadmium pollution soil - Google Patents
A kind of plantation of safe utilization is in the method for the fibre ramie by-product of cadmium pollution soil Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention discloses a kind of plantations of safe utilization in the fibre method of ramie by-product of cadmium pollution soil, includes the following steps:Step S1, in ensiling container be added ensilage, 2% white sugar of ensilage weight, 0.3% salt of ensilage weight, 10% corn flour of ensilage weight, ensilage weight 0.1% lactic acid bacteria, it is compacted, seals, compactness 570kgm after mixing‑3;Step S2, it breaks a seal after sealing 120 days, obtains ensilage.The present invention is by combining additive, compactness, the process conditions such as time that break a seal are designed, show that a kind of fibre that can safely, effectively plant heavy-metal contaminated soil carry out the method that trans-utilization obtains ensilage with ramie by-product, conversion, popularization to ramie by-product Sativa Silage Technology push China ramie to utilize in heavy-metal contaminated soil plantation and by-product highly effective and safe and are of great practical significance.
Description
Technical field
The present invention relates to field of biotechnology, and in particular to a kind of plantation of safe utilization is in the fibre of heavy metal cadmium soil
With the method for ramie by-product.
Background technique
With the fast development of quickening and the industry of urbanization process, agricultural land soil receives various heavy pollution, this
It is showed in southern area of China especially prominent.For large area by, the soil of low heavy metal pollution, in the real feelings of China
To be carried out under condition lie fallow be it is unpractical, as early as possible find effectively repair means reduce Heavy Metal Pollution public health risk
It is the task of top priority.Phytoremediation technology is a kind of ideal heavy metal pollution of soil phytoremediation resource.And it is tired with existing ultraproduct
Plant is compared, and plants within ramie 1 year, harvests for many years, and have degeneration-resistant border ability is strong, growth rapidly, fertility is strong, root system is huge
Greatly, the advantages that biological yield is high, the shortcomings that existing phytoremediation can be made up and deficiency, and harvest product-ramee of ramie
Do not enter food chain, to safety of human and livestock, it can reach and administer soil pollution purpose, and can guarantee higher economic benefit, be
Comparatively ideal heavy metal pollution of soil phytoremediation resource.
The secondary pollution problem that the disposition and further processing of rehabilitation plant are utilized and faced is also urgently to be resolved asks
Topic.With the increase of China's ensilage demand, the grasp of forage grass Sativa Silage Technology is extremely urgent.
Summary of the invention
In view of the deficiencies of the prior art, the present invention is intended to provide a kind of plantation of safe utilization is in heavy metal cadmium soil
The method that fibre uses ramie by-product,
To achieve the goals above, the present invention adopts the following technical scheme that:
A kind of plantation of safe utilization includes the following steps in the fibre method of ramie by-product of cadmium pollution soil:
Step S1, in ensiling container be added ensilage, 2% white sugar of ensilage weight, ensilage weight 0.3%
Salt, 10% corn flour of ensilage weight, ensilage weight 0.1% lactic acid bacteria, be compacted, seal after mixing, compacting
Degree is 570kgm-3;
Step S2, it breaks a seal after sealing 120 days, obtains ensilage.
Further, the ensiling container is tank or Oxford bucket.
The beneficial effects of the present invention are:The present invention by being combined to additive, compactness, the Kaifeng process conditions such as time
It is designed, show that a kind of fibre that can safely, effectively plant heavy-metal contaminated soil be converted with ramie by-product
Using the method for obtaining ensilage, conversion, popularization to ramie by-product Sativa Silage Technology push China ramie heavy metal-polluted
The plantation of dye soil and by-product highly effective and safe, which utilize, to be of great practical significance.
Specific embodiment
The invention will be further described below, it should be noted that before following embodiment is with the technical program
It mentions, the detailed implementation method and specific operation process are given, but protection scope of the present invention is not limited to the present embodiment.
Embodiment one
1, test material
1.1 experimental materials and research method
1.1.1 experimental material
1.1.1.1 material
Test using fibre with ramie by-product as raw material, Liuyang Yonghe County town ramie planting base (Cadmium in Soil 1.0mg/kg) in
In June, 2016 plantation cuttage seeding, 2500 plants/667m2, line-spacing 50cm.On December 2nd, 2016 harvests ramie.The ramie
The cadmium concentration coefficient of kind is below 1.0.
1.1.1.2 ensiling container and specification:
4kg ensilage, compactness 400kgm is added in 10L glass jar-3;40kg ensilage, compacting is added in the Oxford 70L bucket
Degree is 570kgm-3。
1.1.2 research method
1.1.2.1 experimental design
Experiment with compactness, is added using three factor randomized complete-block designs using fibre with ramie by-product as process object
Add agent and the Kaifeng time is variable.2 ensiling device processing of compactness point, glass jar compactness:400kg·m-3, Oxford bucket pressure
Solidity is about 570kgm-3.6 processing are arranged in additive altogether, and glass jar includes:A. non-treated, B.+2% white sugar, C.+2% is white
Sugared+0.3% salt ,+0.1% lactic acid bacteria of+0.3% salt of D.+2% white sugar ,+10% corn flour of+0.3% salt of E.+2% white sugar, F.+
+ 0.1% lactic acid bacteria of+10% corn flour of+0.3% salt of 2% white sugar.Oxford barrel includes:AA. non-treated, BB.+2% white sugar, CC.+
+ 0.3% salt of 2% white sugar ,+0.1% lactic acid bacteria of+0.3% salt of DD.+2% white sugar ,+10% corn of+0.3% salt of EE.+2% white sugar
Powder ,+0.1% lactic acid bacteria of+10% corn flour of+0.3% salt of FF.+2% white sugar.Break a seal the time be 3 processing, after sealing respectively at
The Kaifeng 60d, 90d, 120d.Total 36 processing, each processing set 3 repetitions.
1.1.2.2 ensiling is modulated
For raw material ramie with decorticator by its fiber removal, by-product is cut into 2-3cm long segment through wire kneading machine, is uniformly mixed
(taking the fresh sample of ramie by-product to measure cadmium content before ensiling is 0.77mg/kg).By raw material needed for each mixing ratio weight (according to
1.1.2.1 experimental design) it is weighed into the plastic tub of label respectively, it mixes and is fitted into ensiling container again, mixed
Uniformly.Glass jar ensiling is directly compacted, freshness protection package sealing, then the sealing that closes the lid.Oxford bucket ensiling elder generation film laying, pours into blueness
Bin stock, compacting rear film wrapping, then another laying thin film, above with hydraulic pressure reality.60d, 90d, 120d are stored under normal temperature condition
Kaifeng, obtains ensilage sample.Prepare air-drying sample measurement chemical component and fermentation quality.CP, NDF, ADF and dry matter content
Measurement uses air-dry sample, and pH value measurement uses fresh sample.
1.1.2.3 project measures
1, ensilage sample treatment and sensory evaluation standard
Respectively in 60d, 90d, 120d sampling analysis of ensiling, scored by 4 people scenes, ensiling grade according to《Germany
DLG ensilage sensory evaluation criteria method》Method is evaluated.Including smell, structure and color 3, wherein:Smell point 5
Grade (0~14 point);4 grades (0~4 point) of structure point;3 grades (0~2 point) of color point.Comprehensive 3 item ratings obtain evaluation
As a result:Excellent (20~16 points), good (15~10 points), in (9~5 points) and under (4-0 point) 4 grades.
2, laboratory chemical constituent analysis is specifically evaluated according to the evaluation criteria of ensilage quality in China.
(1) content of pH value:Ensilage sample 45g is taken, 180mI is added, deionized water stirs evenly, in 4 DEG C of refrigerators
It impregnates for 24 hours, successively uses filtered through gauze, filter out careless slag and obtain leachate, then the pH value with pH tester (PHSJ4A) leachate.It is green
Store the judgment criteria of feed pH value:It is excellent:Below pH value 4.0;Well:PH value 4.1-4.3 is general:PH value 4.4-5.0;It is of inferior quality:
PH value .50 or more.
(2) dry matter (DM) content:It is measured using 70 DEG C of oven drying methods, sample is placed in 105 DEG C of water-removing 0.5h of baking oven, then adjust
To dry matter (DM) content of 70 DEG C of dry 48h measurement samples;
(3) crude protein (CP) content, neutral detergent fiber (NDF) and acid detergent fiber (ADF) assay:
Neutral detergent fiber, acid detergent fiber are using the filter bag method measurement after Fan Shi washing fibre method improvement.Crude protein
Content is measured using FOSS full-automatic Kjeldahl determination device Kjeltec 8400.
3, the cadmium content measurement of ensiling ramie dry matter:
Cd element uses nitric acid-perchloric acid wet digestion method in plant sample, and Cd element uses the high chlorine of chloroazotic acid-in pedotheque
Acid disappears cooking method, in prepare liquid Cd content with etc. aas determinations.
(1) plant sample is cleared up:Digestion procedure is nitric acid-perchloric acid wet digestion method resolution.0.5000g- is weighed on demand
For 1.0000g plant sample in 100ml triangular flask, each sample sets three Duplicate Samples, adds 15ml nitric acid-perchloric acid solution (3:1),
It stands overnight, (140-200 heats up by hour) disappears and boil on electric hot plate, emits to the greatest extent to brown cigarette, cooling is removed, as solution still has yellow not
Digestion residual completely, is added 3-5ml perchloric acid, continue to disappear boil to color sample bleach solution it is closely dry until.Remove cooling, mistake
Filter, is settled to 25ml volumetric flask, is filtered with middling speed quantitative filter paper, 10ml is taken to be transferred in centrifuge tube, to be measured.
1.1.3 data statistics and analysis
Data carry out variance statistic point after the preliminary treatment of 2007 statistical softwares of Excel, using 7.0 statistical software of DPS
Analysis.As a result it is indicated with average ± standard deviation and significance analysis.
1.2 results and analysis
1.2.1 the characteristic of raw material
Ramie by-product refers to all parts of the ramie overground part in addition to raw ramie, including sesame slices, boon, fiber crops in this experiment
Shell.Ramie by-product WSC content is 21.34%, NDF 57.47%, and CP content is 15.82%.Its DM content is 20.03%,
I.e. moisture content is 79.97%.
1.2.2 influence of the different additive to ensiling ramie sensory evaluation scores
Table 1 show the Analyses Methods for Sensory Evaluation Results of different compactnesss, ensiling time and additive ensiling ramie by-product.
Table 1
As shown in Table 1, the sensory evaluation scores after the ensiling of each group ramie add white sugar, salt, corn in 11.43~19.0,90d
The sensory evaluation scores highest of powder group, total score 20 belong to quality silage.It totally sees, group containing additive (B, BB, C, CC, D, DD
Group) it is relatively high without additive group (A group, AA group) scoring;With the increase of ensiling number of days, sensory evaluation scores are reduced, each additive group
Ensiling color structure change is not significant, and smell is changed significantly.The color of the ensilage of the group containing additive is in filbert, structure of stem and leaf
It saves preferably, has slight fragrant fruity, without obvious acid smell, silage effect is good;Quality without additive group is slightly sticky, fragrance
Taste is weak, and when arriving 120d, ensiling has apparent acid smell, and mouldy situation occurs in ensiling surface, and silage effect is general.Increase at any time
Add, compactness 570kgm-3Under the conditions of each group compared with 400kgm-3Under the conditions of each group smell, color, without significant difference.
1.2.3 the ensiling influence of different type additive and compactness to ramie by-product Silage Quality in the 60th day
When table 2 show ensiling 60d the additive of heterogeneity and different compactnesss to ensiling ramie fermentation quality and
The experimental result of the influence of chemical component.
Table 2
Note:(P is represented with lowercase difference after column of figure<0.05) level difference is significant.
As shown in table 2, at 60 days, in the ensiling processing group of the additive of heterogeneity and different compactnesss, ensiling
It is in be remarkably decreased (P that ramie by-product pH value, which is relatively free of additive group,>0.05), pH value is between 3.93-6.86.Compactness
570kg·m-3Under the conditions of each group ratio 400kgm-3Under the conditions of each group pH value significantly reduce (P > 0.05).In 400kgm-3Under conditions of compactness, the pH value after each additive group ensiling, which is substantially less than, is free of additive group (P > 0.05), E group pH value
Minimum (4.24), F group take second place (4.26);In 570kgm-3PH value pole under conditions of compactness, after each additive group ensiling
Substantially less than control group (P > 0.05), CC group pH value is minimum (3.93), and FF group takes second place (4.05).
At 60 days, the ensiling processing group ensiling ramie by-product dry matter content of different additive and compactness was in significant
Difference (P>0.05), between 14.97%~25.45%.Wherein, AA group, BB group, EE group, FF group are in compactness 570kgm-3
Under the conditions of than A group, B group, E group, F group in compactness 400kgm-3Under the conditions of dry matter content rise (P > 0.05), CC group, DD
Group is in compactness 570kgm-3Under the conditions of than C group, D group in compactness 400kgm-3Under the conditions of dry matter content decline (P >
0.05).In 400kgm-3Under conditions of compactness, the dry matter content after each additive group ensiling, which is all remarkably higher than to be free of, to be added
Add agent group (P > 0.05), F group dry matter content highest (22.64%), E group takes second place (21.04%);In 570kgm-3Compactness
Under conditions of, the dry matter content after each additive group ensiling is all remarkably higher than without additive group (P > 0.05), FF group dry
Matter content highest (23.01%), EE group take second place (21.72%).
In addition, as seen from Table 2, at 60 days, different additive ensiling processing group ensiling ramie by-product crude protein was manifested
Write difference (P>0.05), different compactnesss influence less, between 13.53%~17.28% each ensiling group crude protein content.
Wherein, AA group, FF group are in compactness 570kgm-3Under the conditions of than A group, F group in compactness 400kgm-3Under conditions of thick egg
Bai Hanliang slightly rises, and BB group, CC group, DD group, EE group are in compactness 570kgm-3Under the conditions of exist than B group, C group, D group, E group
Compactness 400kgm-3Under conditions of crude protein content be slightly decreased.In 400kgm-3Under conditions of compactness, each additive
Crude protein content after group ensiling, which is substantially less than, is free of additive group (P > 0.05), A group crude protein content highest
(16.86%), F group is minimum (14.84%);In 570kgm-3Crude protein under conditions of compactness, after each additive group ensiling
Content is substantially less than and is free of additive group (P > 0.05), AA group highest (17.28%), and DD group is minimum (13.53%).
Further, from table 2 it can also be seen that at 60 days, different additive ensiling processing group ensiling ramie by-product
Object neutral detergent fiber is in significant difference (P>0.05), different compactnesss do not show the influence of each ensiling group neutral detergent fiber content
It writes.In 400kgm-3Under conditions of compactness, the neutral detergent fiber content after each additive group ensiling, which is substantially less than, to be free of
Additive group (P > 0.05), E group neutral detergent fiber content is minimum (33.38%), and F group takes second place (36.49%);In 570kg
m-3Under conditions of compactness, the neutral detergent fiber content after each additive group ensiling, which is substantially less than, is free of additive group (P >
0.05), FF group is minimum (37.28%), and DD group takes second place (41.46%).
1.2.4 the ensiling influence of different type additive and compactness to ramie by-product Silage Quality in the 90th day
The influence of additive and compactness to ensiling ramie fermentation quality and chemical component is real when table 3 show ensiling 90d
Test result.
Table 3
Note:Lowercase difference represents (P after colleague's number<0.05) level difference is significant.
As shown in table 3, at 90 days, the ensiling processing group ensiling ramie by-product pH value of different additive and compactness compared with
It is in extremely significant decline (P without additive group>0.05), pH value is between 3.77-6.61.Compactness 570kgm-3Under the conditions of it is each
Group ratio 400kgm-3Under the conditions of each group pH value significantly reduce (P > 0.05).In 400kgm-3Under conditions of compactness, respectively
PH value after additive group ensiling is extremely significant, and lower than additive group (P > 0.05) is free of, F group pH value is minimum (4.20), and E group is secondary
Its (4.24).In 570kgm-3Under conditions of compactness, the pH value after each additive group ensiling, which is substantially less than, is free of additive
Group (P > 0.05), FF group pH value is minimum (3.77), and DD group takes second place (3.99).
Further, as known from Table 3, at 90 days, the ensiling processing group ensiling ramie pair of different additive and compactness
Product dry matter content is in significant difference (P>0.05), between 16.18%~25.32%.Wherein, DD group, EE group, FF group exist
Compactness 570kgm-3Under the conditions of than D group, E group, F group in compactness 400kgm-3Under the conditions of dry matter content decline (P >
0.05), AA group, BB group, CC group are in compactness 570kgm-3Under the conditions of than A group, B group, C group in compactness 400kgm-3Item
Dry matter content decline (P > 0.05) under part.In 400kgm-3Dry under conditions of compactness, after each additive group ensiling
Matter content is all remarkably higher than without additive group (P > 0.05), F group dry matter content highest (23.26%), and C group is taken second place
(19.14%);In 570kgm-3Under conditions of compactness, the dry matter content after each additive group ensiling is all remarkably higher than not
Group containing additive (P > 0.05), EE group dry matter content highest (23.43%), DD group take second place (16.3%).
Further, as shown in table 3, at 90 days, the thick egg of different additive ensiling processing group ensiling ramie by-product
White is in significant difference (P>0.05), different compactnesss influence less each ensiling group crude protein content, 13.23%~
Between 15.88%.Wherein, BB group, EE group, FF group are in compactness 570kgm-3Under the conditions of than B group, E group, F group in compactness
400kg·m-3Under the conditions of crude protein content slightly rise, DD group is in compactness 570kgm-3Under the conditions of than D group in compactness
400kg·m-3Under the conditions of crude protein content be slightly decreased.In 400kgm-3Under conditions of compactness, after each additive group ensiling
Crude protein content be substantially less than be free of additive group (P > 0.05), A group crude protein content highest (14.86%), F group is most
Low (13.23%);In 570kgm-3Under conditions of compactness, FF histone content highest (15.88%);DD group is minimum
(13.31%).
Still further, as shown in table 3, at 90 days, different additive ensiling processing group ensiling ramie by-product was neutral
Washing fiber is in significant difference (P>0.05), different compactnesss influence each ensiling group neutral detergent fiber content not significant.?
400kg·m-3Under conditions of compactness, the neutral detergent fiber content after each additive group ensiling is substantially less than without addition
Agent group (P > 0.05), E group neutral detergent fiber content is minimum (30.78%), and F group takes second place (34.35%);In 570kgm-3Pressure
Under conditions of solidity, the neutral detergent fiber content after each additive group ensiling, which is substantially less than, is free of additive group (P >
0.05), FF group is minimum (36.71%), and EE group takes second place (39.22%).
1.2.5 the ensiling influence of different type additive and compactness to ramie by-product Silage Quality in the 120th day
The influence of additive and compactness to ensiling ramie fermentation quality and chemical component is real when table 4 show ensiling 120d
Test result.
Table 4
Note:Lowercase difference represents (P after colleague's number<0.05) level difference is significant.
As shown in table 4, at 120 days, the ensiling processing group ensiling ramie by-product pH value of different additive and compactness
It is in relatively extremely significant decline (P without additive group>0.05), pH value is between 3.45-6.69.Compactness 570kgm-3Under the conditions of
Each group ratio 400kgm-3Under the conditions of each group pH value significantly reduce (P > 0.05).In 400kgm-3Under conditions of compactness,
PH value after each additive group ensiling is extremely significant lower than additive group (P > 0.05) is free of, and F group pH value is minimum (3.98), E group
Take second place (4.16);In 570kgm-3Under conditions of compactness, the pH value after each additive group ensiling is substantially less than without addition
Agent group (P > 0.05), FF group pH value is minimum (3.45), and DD group takes second place (3.53).
The ensiling processing group ensiling ramie by-product dry matter content of different additive and compactness is in significant difference (P>
0.05), between 14.97%~25.45%.Wherein, AA group, BB group are in compactness 570kgm-3Under the conditions of exist than A group, B group
Compactness 400kgm-3Under the conditions of dry matter content rise (P > 0.05), CC group, DD group, EE group, FF group are in compactness
570kg·m-3Under the conditions of than C group, D group, E group, F group in compactness 400kgm-3Under the conditions of dry matter content decline (P >
0.05).In 400kgm-3Under conditions of compactness, the dry matter content after each additive group ensiling, which is all remarkably higher than to be free of, to be added
Add agent group (P > 0.05), F group dry matter content highest (25.45%), E group takes second place (25.01%).In 570kgm-3Compactness
Under conditions of, the dry matter content after each additive group ensiling is all remarkably higher than without additive group (P > 0.05), EE group dry
Matter content highest (23.64%), FF group take second place (23.06%).
1.2.6 influence of the different ensiling times to different type additive and compactness ramie by-product Silage Quality
From above-mentioned experimental result it is found that the ensiling time is to different type additive and compactness ramie by-product ensiling pH
Value, neutral detergent fiber, acid detergent fiber content, acid content of lignin have a significant impact.For synthesis, in compactness
400kg·m-3Under the conditions of group, increase with the ensiling time, E group, F group pH value continue to decline, crude protein content, neutral detergent fiber
Content, acid detergent fiber content continue to decline.E group in 120 days Silage Qualities be it is good, F group is excellent in 120 days Silage Qualities
Deng;B group, C group, D group pH value persistently rise, dry matter decline, Silage Quality be it is general, be unfavorable for long term storage;A group is at any time
Variation remains basically stable, and Silage Quality is of inferior quality.In compactness 570kgm-3Under the conditions of, with the ensiling time increase, AA group, DD group,
EE group, FF group pH value are persistently remarkably decreased, and crude protein content continues to decline, and neutral detergent fiber content, acid detergent fiber contain
Amount continues to decline.AA group Silage Quality be it is of inferior quality, DD group, FF group at 90 days Silage Quality be it is excellent, EE group reached in 120 days
Choicest quality.BB group ensiling pH value is fallen before to be risen again, is good in 90 days qualities.CC group ensiling pH value persistently rises, 60
It when Silage Quality be it is excellent.
Comprehensively consider sensory evaluation, the content of pH value, dry matter, content crude protein content, neutral detergent fiber and acidity
The indexs such as fiber content and ensiling time are washed, are found in 570kgm-3Compactness, 2%+0.3% salt of white sugar of addition+10% are beautiful
+ 0.1% lactic acid bacteria of rice flour, Kaifeng time are that can reach best Silage Quality under conditions of 120 days.
1.2.7 the fine safe utilization evaluation with cadmium after the ensiling of ramie by-product
The cadmium content of ramie by-product ensilage is shown in Table 4.Ensilage cadmium content under above-mentioned optimum condition exists
0.96mg/kg.In safety evaluation, it is added in ox daily ration in 100% and following ratio, it is good that ensilage shows as palatability
It is good, it can be ensured that the Permissible limit standard that cadmium content is no more than cadmium content in China's feed in daily ration (meets feed hygiene country mark
Quasi- GB13078-2017, cadmium content≤1.0mg/kg) and carry out safe utilization.
For those skilled in the art, it can be provided various corresponding according to above technical solution and design
Change and modification, and all these change and modification, should be construed as being included within the scope of protection of the claims of the present invention.
Claims (2)
1. a kind of method of fibre ramie by-product of safe utilization plantation in cadmium pollution soil, which is characterized in that including as follows
Step:
Step S1, be added in ensiling container ensilage, 2% white sugar of ensilage weight, ensilage weight 0.3%
Salt, 10% corn flour of ensilage weight, ensilage weight 0.1% lactic acid bacteria, after mixing be compacted, seal, compactness
For 570kgm-3;
Step S2, it breaks a seal after sealing 120 days, obtains ensilage.
2. the method for fibre ramie by-product of the safe utilization plantation according to claim 1 in cadmium pollution soil, special
Sign is that the ensiling container is tank or Oxford bucket.
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CN110396491A (en) * | 2019-08-13 | 2019-11-01 | 湖南农业大学 | A kind of the strain separating identification and Utilization assessment method of degradation of ramie lignocellulosic |
CN110438198A (en) * | 2019-09-05 | 2019-11-12 | 湖南农业大学 | The ramee variety and organ screening technique of antibiotic are prepared based on grass meal extract |
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CN110396491A (en) * | 2019-08-13 | 2019-11-01 | 湖南农业大学 | A kind of the strain separating identification and Utilization assessment method of degradation of ramie lignocellulosic |
CN110438198A (en) * | 2019-09-05 | 2019-11-12 | 湖南农业大学 | The ramee variety and organ screening technique of antibiotic are prepared based on grass meal extract |
CN110438198B (en) * | 2019-09-05 | 2023-07-21 | 湖南农业大学 | Ramie variety and organ screening method for preparing antibiotics based on grass meal extract |
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