CN108849489A - A kind of breeding of high-yield cotton dual purpose lines and application method - Google Patents
A kind of breeding of high-yield cotton dual purpose lines and application method Download PDFInfo
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- CN108849489A CN108849489A CN201810913114.3A CN201810913114A CN108849489A CN 108849489 A CN108849489 A CN 108849489A CN 201810913114 A CN201810913114 A CN 201810913114A CN 108849489 A CN108849489 A CN 108849489A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The present invention is female parent using single stealthy gms two-line system material GA18 sterile plant, with high yield upland cotton nasal mucus cotton No. 3 for male parent, offspring isolates fertile plant and sterile plant, and fertile plant and sterile plant brother and sister is constantly selected to hand over, and selects character and stablizes, sterile plant and fertile plant respectively account for the 50% dual-purpose based material of sterile cotton, the dual purpose lines have, high yield, more anti-, preferable characteristics of quality, stable fertility, restorer is restorative good, it is easier to filter out excellent combination.Dual-purpose cotton crossbreed production method of the invention a kind of has suitable compared with conventional hybridization kind yield, does not need artificial emasculation, hybrid seeding saving of labor 60%, floral organ does not come to harm, the higher advantage of Boll probability.The presence that bell is selfed in hybrid seeding can effectively be controlled, preventing and treating adulterated makes false phenomenon, improve the cotton hybrid seed quality of production, it is produced and sold for hybrid cotton and technical support is provided, to safeguard the legitimate rights and interests of Seed enterprises and peasant, promote the sound development of Cotton Industry, there is very high application value.
Description
Technical field
The invention belongs to crop breeding technique field, the especially a kind of breeding of high-yield cotton dual purpose lines and application method.
Background technique
Since promoting transgenic cotton against pests, bollworm-resistance hybrid cotton can be quickly by merits such as high yield, high-quality and insect resistaces
It condenses together, is widely used in the Yangtze river basin and Yellow River basin cotton region.Cenospecies production, which is mainly taken, manually goes
Male mode, artificial emasculation cenospecies large labor intensity, recruitment are more, and production of hybrid seeds expense increases year by year, and hybrid seeding is caused to encounter bottleneck.
The cost of artificial emasculation can be saved using cotton sterile male line.
At present in terms of cotton " three systems " and " two systems " crossbreeding, Sichuan Province utilizes single hidden line with genic sterile " hole A type
(ms14) ", Handan academy of agricultural sciences utilizes " three systems " sterile line, the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute and Agricultural University Of Nanjing to utilize
Double-genic-male-sterile line (ms5ms5ms6ms6) has selected new cotton variety, and India breeder cultivates hybridization using ms5ms6
Cotton variety Sugana.Since restoring gene strong in upland cotton is few and recovery resource is narrow, the breeding difficulty of restorer is big.
The present invention by the single stealthy kernel sterility dual serial initiative of research, the screening of male-sterile seed production and cross combination,
Hybrid seeding cost can be saved, production of hybrid seeds program is simplified, effectively controls and is selfed bell in hybrid seeding, prevention and treatment is adulterated to make false phenomenon,
The cotton hybrid seed quality of production is improved, technical support is provided for hybrid cotton producing and selling, to safeguard Seed enterprises and peasant
Legitimate rights and interests promote the sound development of Cotton Industry, have very high application value.
Summary of the invention
The present invention is directed to formulate the dual-purpose based material of new cotton, screening high-yield hybrid combination, production using genic male sterile gene
Cross combination, and participate in area's examinations at different levels and Combination nova experiment and demonstration.
In order to achieve the above purpose, the present invention provides following technical scheme：
A kind of breeding of high-yield cotton dual purpose lines and application method, include the following steps：
(1) breeding of high-yield cotton kernel sterility dual serial material；
(2) high-yield cotton dual purpose lines male-sterile seed produces；
(3) screening of high-yield cotton kernel sterility dual serial cross combination；
(4) hybrid seed F1 experiment and demonstration.
It is female parent that selection, which is using kernel sterility dual serial GA18 sterile plant, in the step 1, utilizes high-production synthetic
Shape excellent upland cotton nasal mucus cotton No. 3 are paternal hybrid, and F1 generation selfing is mixed to receive, and F2 is 1 for sterile plant and fertile plant segregation ratio:3,
Select plant type is good, the thorough sterile plant of pollen abortion be it is maternal, select pollen amount foot, plant type are compact, tie the strong fertile plant of bell for
Male parent carry out to strain brother and sister hand over, F3 to F6 generation continue F2 for the step of, select brother and sister to strain friendship, abortion is not thorough, have it is micro
Pollen eliminates strain, to select good strains in the field for seed select a time between cooperate it is disease-resistant detection and sulfuric acid kalamycin detect, eliminate disease-resistant, pest-resistant
Property difference to strain, retaining has pest-resistant, disease-resistant, high yield and fertility good to strain, strain is handed over by the brother and sister of several generations, sterile plant and
Fertile plant ratio reaches 1:1, each inhereditary feature is basicly stable, retains 10 to strain, carries out combo test as dual purpose lines.
The high yield kernel sterility dual serial has plant height medium, and loosely, medium blade, stalk villus is medium for fruit branch, resists
Worm, the characteristic of anti-blight, resistance to verticillium wilt.
Production method is to sow kernel sterility dual serial material according to 2500-3000 plants per acre in the step 2, wait bloom
Phase checks 2/3 extracted in fertile plant, retains sterile plant and fertile plant ratio is 3:1, pollen is collected from fertile plant, to infertility
Strain pollination, is identified using sulfuric acid kalamycin, is extracted to reacting positive strain, not pre- strain loose powder situation is checked, to a small amount of loose powder strain
It extracts.
Screening technique is preferable with character to be female parent with the dual-purpose based material sterile plant of sterile cotton in the step 3
Upland cotton is male parent, configures cross combination, and the combination F1 generation of configuration carries out variety test, investigates the yield of kind, resistance
And fiber quality, filter out the mostly anti-cross combination of high yield and high quality.
It is the combination production of hybrid seeds that will be screened that demonstration methods are tested in the step 4, the fertile plant in dual purpose lines is extracted, using not
Strain configuration cross combination is educated, area's examinations at different levels, and experiment and demonstration of layouting are participated in cross combination.
It is an advantage of the invention that：
The present invention is female parent using single stealthy gms two-line system material GA18 sterile plant, is with high yield upland cotton nasal mucus cotton No. 3
Male parent, offspring isolate fertile plant and sterile plant, and fertile plant and sterile plant brother and sister is constantly selected to hand over, and select character and stablize, no
The dual-purpose based material of sterile cotton that strain and fertile plant respectively account for 50% is educated, which has, and high yield, mostly anti-, quality is preferable
Characteristic, stable fertility, restorer is restorative good, there is no three be restorer difficulty screening, recovery rate it is not high the problem of, it is easier to
Filter out excellent combination.Cross combination is configured using material, the characters such as high yield, high-quality, how anti-is shown, there is stronger advantage.
Dual-purpose cotton crossbreed production method of the invention a kind of has suitable compared with conventional hybridization kind yield, does not need artificial emasculation, miscellaneous
Production of hybrid seeds saving of labor 60% is handed over, floral organ does not come to harm, the higher advantage of Boll probability, can effectively control and be selfed bell in hybrid seeding
In the presence of, prevention and treatment is adulterated to make false phenomenon, improves the cotton hybrid seed quality of production, and it is produced and sold for hybrid cotton and technical support is provided,
To safeguard the legitimate rights and interests of Seed enterprises and peasant, promote the sound development of Cotton Industry, there is very high application value.
Detailed description of the invention
Fig. 1 show the dual-purpose based material initiative of sterile cotton, male-sterile seed production and cross combination configuration technology road
Line chart.
Fig. 2 show the dual-purpose based material sterile plant single plant of sterile cotton.
Fig. 3 show the dual-purpose based material sterile plant flower form of sterile cotton, and anther is normal, no pollen.
Fig. 4 show the Cross Combinations F1 using the dual-purpose based material configuration of sterile cotton.
Specific embodiment
Embodiment one
Anqing in 2012 uses kernel sterility dual serial GA18 sterile plant for female parent, and nasal mucus cotton No. 3 configure hybridization group for male parent
It closes.
Hainan F1 closing selfing in 2012, harvests 564 grams of seed.
Anqing F2 in 2013 isolates sterile plant and fertile plant, ratio 1:3, disease-resistant detection, four leaf stage sulfuric acid OK a karaoke club is mould
Element spray Newborn Leaves, select disease-resistant, tension mycin reaction negative and plant type loosely, the thorough sterile single plant of Anther Abortion, selection
Anther is full, and the strong single plant of knot bell, which is done, hybridizes strain, configures to strain 30, single cotton boll received in sterile plant.
Hainan in 2013, divides single-strain planting F3, every disease-resistant detection of plant, and four leaf stage sulfuric acid kalamycin sprays Newborn Leaves, choosing
Select disease-resistant, tension mycin reaction negative and plant type loosely, the thorough sterile single plant of Anther Abortion selects anther full, ties bell
Property strong single plant do strain hybridized, superseded abortion is not thorough or insect resistance identification is impure to plant 20, is showing preferable plant
Choosing hands over 30 to strain brother and sister, single cotton boll received in sterile plant.
Single-strain planting F4 is divided in Anqing within 2014, and step is same as above, and choosing, which is done, hands over 20 to strain brother and sister.
Single-strain planting F5 is divided in Hainan within 2015, and step is same as above, and choosing, which is done, hands over 15 to strain brother and sister.
Single plant F6 is planted in Anqing in 2015, investigates sterile plant and fertile plant segregation ratio, and selection percentage is close to 1:1 plant
Row, Resistant identification are same as above, retain 10 plant complete stool row sterile plants and fertile plant brother and sister acceptance to obtain infertility two be seed.
Hainan single-strain planting point plantation in 2015, the sterile plant of 10 plants hybridize with middle plant cotton No. 2 respectively, harvest hybridization
Kind F1.
Anqing in 2016 carries out variety test to F1, and test data is as follows：
From the point of view of variety test result, the cross combination yield of No. 3 sterile line configuration is high, pest-resistant, disease resistance is good,
Fiber quality is preferable, no sterile plant.
Embodiment two
Male-sterile seed production.
Hainan in 2016, dual-purpose based material 3 253 plants of plantations, florescence investigation fertility, 125 plants of sterile plant, fertile plant
128 plants, insect resistance identification is not 3 plants of strain pest-resistant, pulls out, and 10 plants of weak plant is pulled out according to principle as strict as possible, in order to allow sterile plant to have more preferably
Growing environment, extract 88 plants of fertile plant, it is practical to retain 119 plants of sterile plant, 40 plants of fertile plant, harvest male-sterile seed 1254
Gram.
Embodiment three
Dual purpose male-sterile line material × hybridization the F1 of middle plant cotton No. 2 production.
Strain number 1224, according to normal 2 double density, is planted in the ground of another piece of Hainan in 2016 by 0.3 mu of planting material dual purpose lines
12 plants of strain not pest-resistant, 24 plants of weak plant, 612 plants of fertile plant, practical to retain 576 plants of sterile plant, plant cotton 2 in 103 plants of plantation is pulled out in strain
Number, complete stool hybridization harvests 14.5 kilograms of hybrid seed.
Example IV
Dual purpose male-sterile line material × hybridization F1 the experiment and demonstration of middle plant cotton No. 2.
3 experimental tests demonstration is arranged in Anqing, Wangjiang and Hefei within 2017, time result see the table below：
Claims (6)
1. breeding and the application method of a kind of high-yield cotton dual purpose lines, which is characterized in that include the following steps：
（1）The breeding of high-yield cotton kernel sterility dual serial material；
（2）The production of high-yield cotton dual purpose lines male-sterile seed；
（3）The screening of high-yield cotton kernel sterility dual serial cross combination；
（4）Hybrid seed F1 experiment and demonstration.
2. breeding and the application method of high-yield cotton dual purpose lines according to claim 1, which is characterized in that the step 1
It is female parent that middle selection, which is using kernel sterility dual serial GA18 sterile plant, utilizes the excellent upland cotton nasal mucus cotton of high-production synthetic character
No. 3 are paternal hybrid, and F1 generation selfing is mixed to receive, and F2 is 1 for sterile plant and fertile plant segregation ratio:3, selection plant type is good, pollen loses
Thorough sterile plant is educated for female parent, and pollen amount foot, the fertile plant that plant type is compact, knot bell is strong is selected to carry out for male parent to strain brother and sister
Hand over, F3 to F6 generation continue F2 for the step of, select brother and sister to strain friendship, abortion is not thorough, has being washed in a pan to strain for trace-pollen
Eliminate, to select good strains in the field for seed select a time between cooperate disease-resistant detection and sulfuric acid kalamycin to detect, it is poor to strain to eliminate disease-resistant, insect resistace, retains
It is good to strain with pest-resistant, disease-resistant, high yield and fertility, strain is handed over by the brother and sister of several generations, sterile plant and fertile plant ratio reach
1:1, each inhereditary feature is basicly stable, retains 10 to strain, carries out combo test as dual purpose lines.
3. breeding and the application method of high-yield cotton dual purpose lines according to claim 1 or 2, which is characterized in that high-yield nucleic
Sterile dual-purpose has plant height medium, and loosely, medium blade, stalk villus is medium for fruit branch, and pest-resistant, anti-blight, resistance to Huang wither
The characteristic of disease.
4. breeding and the application method of high-yield cotton dual purpose lines according to claim 1, which is characterized in that the step 2
Middle production method is to sow kernel sterility dual serial material according to 2500-3000 plants per acre, to florescence, checks and extracts fertile plant
In 2/3, retaining sterile plant and fertile plant ratio is 3:1, pollen is collected from fertile plant, pollinates to sterile plant, utilizes sulfuric acid card
Mycin identification is drawn, reacting positive strain is extracted, checks not pre- strain loose powder situation, a small amount of loose powder strain is extracted.
5. breeding and the application method of high-yield cotton dual purpose lines according to claim 1, which is characterized in that the step 3
It is female parent that middle screening technique, which is with the dual-purpose based material sterile plant of sterile cotton, using the preferable upland cotton of character as male parent, configuration
The combination F1 generation of cross combination, configuration carries out variety test, investigates the yield of kind, resistance and fiber quality, filters out
The mostly anti-cross combination of high yield and high quality.
6. breeding and the application method of high-yield cotton dual purpose lines according to claim 1, which is characterized in that the step 4
Middle test demonstration methods are the combination production of hybrid seeds that will be screened, and extract the fertile plant in dual purpose lines, configure cross combination using sterile plant,
Area's examinations at different levels, and experiment and demonstration of layouting are participated in cross combination.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111972281A (en) * | 2020-08-27 | 2020-11-24 | 四川省农业科学院经济作物育种栽培研究所 | Breeding method of cotton nuclear sterile dual-purpose line |
| CN112005876A (en) * | 2019-05-30 | 2020-12-01 | 四川省农业科学院经济作物育种栽培研究所 | Method for breeding disease and insect resistant high quality cotton variety by using cotton nuclear sterile hybrid |
| CN112470918A (en) * | 2020-12-18 | 2021-03-12 | 四川省农业科学院经济作物育种栽培研究所 | Breeding method and application of transgenic insect-resistant high-coat-content cotton nuclear sterile dual-purpose line |
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2018
- 2018-08-13 CN CN201810913114.3A patent/CN108849489A/en active Pending
Patent Citations (2)
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| CN1138467C (en) * | 2000-08-09 | 2004-02-18 | 四川省农业科学院经济作物育种栽培研究所 | Method for preparing disease-resistant nuclear-sterile dual-purpose line and hybrid of cotton |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112005876A (en) * | 2019-05-30 | 2020-12-01 | 四川省农业科学院经济作物育种栽培研究所 | Method for breeding disease and insect resistant high quality cotton variety by using cotton nuclear sterile hybrid |
| CN111972281A (en) * | 2020-08-27 | 2020-11-24 | 四川省农业科学院经济作物育种栽培研究所 | Breeding method of cotton nuclear sterile dual-purpose line |
| CN112470918A (en) * | 2020-12-18 | 2021-03-12 | 四川省农业科学院经济作物育种栽培研究所 | Breeding method and application of transgenic insect-resistant high-coat-content cotton nuclear sterile dual-purpose line |
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