CN108841766A - A kind of preparation method of the fresh-keeping bacteria strain of microorganism - Google Patents
A kind of preparation method of the fresh-keeping bacteria strain of microorganism Download PDFInfo
- Publication number
- CN108841766A CN108841766A CN201810840924.0A CN201810840924A CN108841766A CN 108841766 A CN108841766 A CN 108841766A CN 201810840924 A CN201810840924 A CN 201810840924A CN 108841766 A CN108841766 A CN 108841766A
- Authority
- CN
- China
- Prior art keywords
- strain
- bacterial strain
- fresh
- temperature
- microorganism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of preparation methods of the fresh-keeping bacteria strain of microorganism, the fresh-keeping bacteria strain of microorganism is made using left lactic acid bacillus bacterial strain, it can directly be sprayed on fruit, reduce the rate of corrosion of fruit, extend the shelf-life of fruit, it can keep the taste and color of fruit, it is harmless, it is green and healthy, it is readily cleaned, preparation method simple possible, has a extensive future.
Description
Technical field
The present invention relates to food fresh keeping bacterium technical fields, and in particular to a kind of preparation method of the fresh-keeping bacteria strain of microorganism.
Background technique
Vegetable and fruit is essential in daily life, and the freshness date of vegetables and fruits is generally shorter, from picking to selling in the presence of
Between difference, how to improve vegetables and fruits freshness, improve consumer desire to buy be one of problem of many businessman's headaches.It is existing
Antistaling agent mostly uses greatly chemical substance, to hidden danger existing for human body not it is found that the microbial bacteria progress using food-grade is fresh-keeping both
Environment-friendly and green there will not be compared with major injury human body.
Summary of the invention
In order to solve the problems existing in the prior art, the present invention provides a kind of preparation method of the fresh-keeping bacteria strain of microorganism,
Suitable for can directly be sprayed on such as banana melon and fruit to the fresh-keeping of melon and fruit, fungistatic effect is obvious, harmless, green, strong
Health, environmental protection are readily cleaned clean.
To achieve the above object, the technical solution adopted by the present invention is：
A kind of preparation method of the fresh-keeping bacteria strain of microorganism, includes the following steps：
Step 1: left lactic acid bacillus strain inoculated is being maintained 30 DEG C of temperature conditions on liquid bacterial strain culture medium flat plate
18~21h of lower constant temperature incubation；
Step 2: the bacterium solution that step 1 is obtained is inoculated on fluid nutrient medium plate, shaking table under the conditions of maintaining 30 DEG C of temperature
18~48h of shaken cultivation observes growing state；
Step 3: strain inoculated is subjected to shaking table shaken cultivation in seed culture medium when strain growth to logarithmic growth phase,
Temperature is controlled at 28~31 DEG C, is put into sterile centrifugation tube after cultivating 18~20h and is carried out centrifugal treating for a period of time, in abandoning
Clearly, bacterial strain is collected, with sterile water washing bacterial strain, using sterile water to adjust the OD value of bacterial strain is 0.6~0.7 fresh-keeping as microorganism
Bacteria strain.
Further, include the following steps：
Step 1: left lactic acid bacillus strain inoculated is being maintained 30 DEG C of temperature on 250mL liquid bacterial strain culture medium flat plate
Under the conditions of constant temperature incubation 20h；
Step 2: the bacterium solution that step 1 is obtained is inoculated on 250mL fluid nutrient medium plate, under the conditions of maintaining 30 DEG C of temperature
Shaking table shaken cultivation 48h observes growing state；
Step 3: strain inoculated is carried out shaking table oscillation in 250mL seed culture medium when strain growth to logarithmic growth phase
Culture, temperature are controlled at 30 DEG C, and progress centrifugal treating 10min in sterile centrifugation tube is put into after cultivating 20h, abandons supernatant, is collected
Bacterial strain, with sterile water washing bacterial strain, using sterile water to adjust the OD value of bacterial strain is 0.6 as the fresh-keeping bacteria strain of microorganism.
Further, include the following steps：
Step 1: left lactic acid bacillus strain inoculated is being maintained 30 DEG C of temperature conditions on liquid bacterial strain culture medium flat plate
18~21h of lower constant temperature incubation；
Step 2: the bacterium solution that step 1 is obtained is inoculated on fluid nutrient medium plate, shaking table under the conditions of maintaining 30 DEG C of temperature
Shaken cultivation observes growing state, and OD600 value is surveyed in sampling after cultivating 18~48h, does not connect the fluid nutrient medium plate conduct of bacterial strain
Comparison group；
Step 3: the OD value for measuring bacterial strain is 0.5~0.7, when strain growth to logarithmic growth phase, bacterial strain is put into seed culture
In base carry out shaking table shaken cultivation, temperature control at 28~31 DEG C, be put into after cultivating 18~20h in sterile centrifugation tube carry out from
The heart handles 9~12min, abandons supernatant, collects bacterial strain, three times with sterile water washing bacterial strain, the OD value of bacterial strain is adjusted using sterile water
The fresh-keeping bacteria strain of microorganism is used as 0.6~0.7.
Further, include the following steps：
Step 1: left lactic acid bacillus strain inoculated is being maintained 30 DEG C of temperature on 250mL liquid bacterial strain culture medium flat plate
Under the conditions of constant temperature incubation 20h；
Step 2: the bacterium solution that step 1 is obtained is inoculated on 250mL fluid nutrient medium plate, under the conditions of maintaining 30 DEG C of temperature
Shaking table shaken cultivation observes growing state, and OD600 value is surveyed in sampling afterwards for 24 hours for culture, does not connect the fluid nutrient medium plate conduct of bacterial strain
Comparison group；
Step 3: the OD value for measuring bacterial strain is 0.5~0.7, when strain growth to logarithmic growth phase, bacterial strain is put into 250mL kind
Shaking table shaken cultivation is carried out in sub- culture medium, temperature controls at 30 DEG C, is put into sterile centrifugation tube and is centrifuged after cultivating 20h
10min is handled, supernatant is abandoned, collects bacterial strain, the use of the OD value that sterile water adjusts bacterial strain is 0.6 three times with sterile water washing bacterial strain
As the fresh-keeping bacteria strain of microorganism.
Further, in step 1, the group of the liquid strain cultures is divided into：Beef extract 3~4%, metasilicic acid 1
~2%, glucose 0.5~2%, sucrose 0.5~1.5%, millet flour 1~2%, peptone 2~3%, sodium chloride 0.5~1%,
Calcium chloride 1-2%, single water manganese sulfate 0.5~1.5%, remaining is sterile water, pH value 7.0.
Further, in step 2, the group of the fluid nutrient medium is divided into：Soybean powder 3~4%, metasilicic acid 1~2%, glucose
0.5~2%, corn flour 1~2%, peptone 2~3%, two hypophosphite monohydrate disodium hydrogens 0.5~1%, calcium chloride 1-2%, single water sulphur
Sour manganese 0.5~1.5%, remaining is sterile water, pH value 7.0.
Further, in step 3, the group of the seed culture medium is divided into：Beef extract 3~4%, corn flour 1~2%,
Glucose 0.5~2%, yeast powder 0.5~1.5%, millet flour 1~2%, peptone 2~3%, two hypophosphite monohydrate disodium hydrogens
0.5~1%, single water manganese sulfate 0.5~1.5%, remaining is sterile water, pH value 7.0.
Compared with prior art, beneficial effects of the present invention are：
The invention discloses a kind of preparation methods of the fresh-keeping bacteria strain of microorganism, and micro- life is made using left lactic acid bacillus bacterial strain
The fresh-keeping bacteria strain of object, can directly be sprayed on fruit, reduce the rate of corrosion of fruit, extend the shelf-life of fruit, can keep water
The taste and color of fruit, harmless, green and healthy, preparation method simple possible has a extensive future.
Specific embodiment
The present invention will be further explained combined with specific embodiments below.
Embodiment 1
A kind of preparation method of the fresh-keeping bacteria strain of microorganism, includes the following steps：
Step 1: the left lactic acid bacillus strain inoculated that market is commercially available is being tieed up on liquid bacterial strain culture medium flat plate
Hold 18~21h of constant temperature incubation under the conditions of 30 DEG C of temperature；
Step 2: the bacterium solution that step 1 is obtained is inoculated on fluid nutrient medium plate, shaking table under the conditions of maintaining 30 DEG C of temperature
18~48h of shaken cultivation observes growing state；
Step 3: strain inoculated is subjected to shaking table shaken cultivation in seed culture medium when strain growth to logarithmic growth phase,
Temperature is controlled at 28~31 DEG C, is put into sterile centrifugation tube after cultivating 18~20h and is carried out centrifugal treating for a period of time, in abandoning
Clearly, bacterial strain is collected, with sterile water washing bacterial strain, using sterile water to adjust the OD value of bacterial strain is 0.6~0.7 fresh-keeping as microorganism
Bacteria strain.
In step 1, the group of liquid strain cultures is divided into：Beef extract 3~4%, metasilicic acid 1~2%, glucose
0.5~2%, sucrose 0.5~1.5%, millet flour 1~2%, peptone 2~3%, sodium chloride 0.5~1%, calcium chloride 1-2%,
Single water manganese sulfate 0.5~1.5%, remaining is sterile water, pH value 7.0.
In step 2, the group of fluid nutrient medium is divided into：Soybean powder 3~4%, metasilicic acid 1~2%, glucose 0.5~2%, corn
Powder 1~2%, peptone 2~3%, two hypophosphite monohydrate disodium hydrogens 0.5~1%, calcium chloride 1-2%, single water manganese sulfate 0.5~
1.5%, remaining is sterile water, pH value 7.0.
In step 3, the group of seed culture medium is divided into：Beef extract 3~4%, corn flour 1~2%, glucose 0.5~
2%, yeast powder 0.5~1.5%, millet flour 1~2%, peptone 2~3%, two hypophosphite monohydrate disodium hydrogens 0.5~1%, single water sulphur
Sour manganese 0.5~1.5%, remaining is sterile water, pH value 7.0.
Embodiment 2
A kind of preparation method of the fresh-keeping bacteria strain of microorganism, includes the following steps：
Step 1: left lactic acid bacillus strain inoculated is being maintained 30 DEG C of temperature on 250mL liquid bacterial strain culture medium flat plate
Under the conditions of constant temperature incubation 20h；
Step 2: the bacterium solution that step 1 is obtained is inoculated on 250mL fluid nutrient medium plate, under the conditions of maintaining 30 DEG C of temperature
Shaking table shaken cultivation 48h observes growing state；
Step 3: strain inoculated is carried out shaking table oscillation in 250mL seed culture medium when strain growth to logarithmic growth phase
Culture, temperature are controlled at 30 DEG C, and progress centrifugal treating 10min in sterile centrifugation tube is put into after cultivating 20h, abandons supernatant, is collected
Bacterial strain, with sterile water washing bacterial strain, using sterile water to adjust the OD value of bacterial strain is 0.6 as the fresh-keeping bacteria strain of microorganism.
Remaining same embodiment 1.
Embodiment 3
A kind of preparation method of the fresh-keeping bacteria strain of microorganism, includes the following steps：
Step 1: left lactic acid bacillus strain inoculated is being maintained 30 DEG C of temperature conditions on liquid bacterial strain culture medium flat plate
18~21h of lower constant temperature incubation；
Step 2: the bacterium solution that step 1 is obtained is inoculated on fluid nutrient medium plate, shaking table under the conditions of maintaining 30 DEG C of temperature
Shaken cultivation observes growing state, and OD600 value is surveyed in sampling after cultivating 18~48h, does not connect the fluid nutrient medium plate conduct of bacterial strain
Comparison group；
Step 3: the OD value for measuring bacterial strain is 0.5~0.7, when strain growth to logarithmic growth phase, bacterial strain is put into seed culture
In base carry out shaking table shaken cultivation, temperature control at 28~31 DEG C, be put into after cultivating 18~20h in sterile centrifugation tube carry out from
The heart handles 9~12min, abandons supernatant, collects bacterial strain, three times with sterile water washing bacterial strain, the OD value of bacterial strain is adjusted using sterile water
The fresh-keeping bacteria strain of microorganism is used as 0.6~0.7.
Remaining same embodiment 1.
Embodiment 4
A kind of preparation method of the fresh-keeping bacteria strain of microorganism, includes the following steps：
Step 1: left lactic acid bacillus strain inoculated is being maintained 30 DEG C of temperature on 250mL liquid bacterial strain culture medium flat plate
Under the conditions of constant temperature incubation 20h；
Step 2: the bacterium solution that step 1 is obtained is inoculated on 250mL fluid nutrient medium plate, under the conditions of maintaining 30 DEG C of temperature
Shaking table shaken cultivation observes growing state, and OD600 value is surveyed in sampling afterwards for 24 hours for culture, does not connect the fluid nutrient medium plate conduct of bacterial strain
Comparison group；
Step 3: the OD value for measuring bacterial strain is 0.5~0.7, when strain growth to logarithmic growth phase, bacterial strain is put into 250mL kind
Shaking table shaken cultivation is carried out in sub- culture medium, temperature controls at 30 DEG C, is put into sterile centrifugation tube and is centrifuged after cultivating 20h
10min is handled, supernatant is abandoned, collects bacterial strain, the use of the OD value that sterile water adjusts bacterial strain is 0.6 three times with sterile water washing bacterial strain
As the fresh-keeping bacteria strain of microorganism.
Remaining same embodiment 1.
The fresh-keeping bacteria strain of microorganism prepared by embodiment 1-4 is sprayed on respectively on the banana just picked, and does not spray bacterium
The banana of strain is compared, and the shelf-life of the banana after spraying the fresh-keeping bacteria strain of microorganism can extend 2-3 days, and taste and color variation are not
Obviously.
The above embodiments do not limit the invention in any form, all to be obtained by the way of equivalent substitution or equivalent transformation
Technical solution, all fall within protection scope of the present invention.
Claims (7)
1. a kind of preparation method of the fresh-keeping bacteria strain of microorganism, which is characterized in that include the following steps：
Step 1: left lactic acid bacillus strain inoculated is being maintained 30 DEG C of temperature conditions on liquid bacterial strain culture medium flat plate
18~21h of lower constant temperature incubation；
Step 2: the bacterium solution that step 1 is obtained is inoculated on fluid nutrient medium plate, shaking table under the conditions of maintaining 30 DEG C of temperature
18~48h of shaken cultivation observes growing state；
Step 3: strain inoculated is subjected to shaking table shaken cultivation in seed culture medium when strain growth to logarithmic growth phase,
Temperature is controlled at 28~31 DEG C, is put into sterile centrifugation tube after cultivating 18~20h and is carried out centrifugal treating for a period of time, in abandoning
Clearly, bacterial strain is collected, with sterile water washing bacterial strain, using sterile water to adjust the OD value of bacterial strain is 0.6~0.7 fresh-keeping as microorganism
Bacteria strain.
2. a kind of preparation method of the fresh-keeping bacteria strain of microorganism according to claim 1, which is characterized in that including following step
Suddenly：
Step 1: left lactic acid bacillus strain inoculated is being maintained 30 DEG C of temperature on 250mL liquid bacterial strain culture medium flat plate
Under the conditions of constant temperature incubation 20h；
Step 2: the bacterium solution that step 1 is obtained is inoculated on 250mL fluid nutrient medium plate, under the conditions of maintaining 30 DEG C of temperature
Shaking table shaken cultivation 48h observes growing state；
Step 3: strain inoculated is carried out shaking table oscillation in 250mL seed culture medium when strain growth to logarithmic growth phase
Culture, temperature are controlled at 30 DEG C, and progress centrifugal treating 10min in sterile centrifugation tube is put into after cultivating 20h, abandons supernatant, is collected
Bacterial strain, with sterile water washing bacterial strain, using sterile water to adjust the OD value of bacterial strain is 0.6 as the fresh-keeping bacteria strain of microorganism.
3. a kind of preparation method of the fresh-keeping bacteria strain of microorganism, which is characterized in that include the following steps：
Step 1: left lactic acid bacillus strain inoculated is being maintained 30 DEG C of temperature conditions on liquid bacterial strain culture medium flat plate
18~21h of lower constant temperature incubation；
Step 2: the bacterium solution that step 1 is obtained is inoculated on fluid nutrient medium plate, shaking table under the conditions of maintaining 30 DEG C of temperature
Shaken cultivation observes growing state, and OD600 value is surveyed in sampling after cultivating 18~48h, does not connect the fluid nutrient medium plate conduct of bacterial strain
Comparison group；
Step 3: the OD value for measuring bacterial strain is 0.5~0.7, when strain growth to logarithmic growth phase, bacterial strain is put into seed culture
In base carry out shaking table shaken cultivation, temperature control at 28~31 DEG C, be put into after cultivating 18~20h in sterile centrifugation tube carry out from
The heart handles 9~12min, abandons supernatant, collects bacterial strain, three times with sterile water washing bacterial strain, the OD value of bacterial strain is adjusted using sterile water
The fresh-keeping bacteria strain of microorganism is used as 0.6~0.7.
4. a kind of preparation method of the fresh-keeping bacteria strain of microorganism according to claim 3, which is characterized in that including following step
Suddenly：
Step 1: left lactic acid bacillus strain inoculated is being maintained 30 DEG C of temperature on 250mL liquid bacterial strain culture medium flat plate
Under the conditions of constant temperature incubation 20h；
Step 2: the bacterium solution that step 1 is obtained is inoculated on 250mL fluid nutrient medium plate, under the conditions of maintaining 30 DEG C of temperature
Shaking table shaken cultivation observes growing state, and OD600 value is surveyed in sampling afterwards for 24 hours for culture, does not connect the fluid nutrient medium plate conduct of bacterial strain
Comparison group；
Step 3: the OD value for measuring bacterial strain is 0.5~0.7, when strain growth to logarithmic growth phase, bacterial strain is put into 250mL kind
Shaking table shaken cultivation is carried out in sub- culture medium, temperature controls at 30 DEG C, is put into sterile centrifugation tube and is centrifuged after cultivating 20h
10min is handled, supernatant is abandoned, collects bacterial strain, the use of the OD value that sterile water adjusts bacterial strain is 0.6 three times with sterile water washing bacterial strain
As the fresh-keeping bacteria strain of microorganism.
5. a kind of preparation method of the fresh-keeping bacteria strain of microorganism according to claim 1 or 3, which is characterized in that step 1
In, the group of the liquid strain cultures is divided into：Beef extract 3~4%, metasilicic acid 1~2%, glucose 0.5~2%, sucrose
0.5~1.5%, millet flour 1~2%, peptone 2~3%, sodium chloride 0.5~1%, calcium chloride 1-2%, single water manganese sulfate 0.5
~1.5%, remaining is sterile water, pH value 7.0.
6. a kind of preparation method of the fresh-keeping bacteria strain of microorganism according to claim 1 or 3, which is characterized in that step 2
In, the group of the fluid nutrient medium is divided into：Soybean powder 3~4%, metasilicic acid 1~2%, glucose 0.5~2%, corn flour 1~
2%, peptone 2~3%, two hypophosphite monohydrate disodium hydrogens 0.5~1%, calcium chloride 1-2%, single water manganese sulfate 0.5~1.5%, remaining
For sterile water, pH value 7.0.
7. a kind of preparation method of the fresh-keeping bacteria strain of microorganism according to claim 1 or 3, which is characterized in that step 3
In, the group of the seed culture medium is divided into：Beef extract 3~4%, corn flour 1~2%, glucose 0.5~2%, yeast powder
0.5~1.5%, millet flour 1~2%, peptone 2~3%, two hypophosphite monohydrate disodium hydrogens 0.5~1%, single water manganese sulfate 0.5~
1.5%, remaining is sterile water, pH value 7.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810840924.0A CN108841766A (en) | 2018-07-27 | 2018-07-27 | A kind of preparation method of the fresh-keeping bacteria strain of microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810840924.0A CN108841766A (en) | 2018-07-27 | 2018-07-27 | A kind of preparation method of the fresh-keeping bacteria strain of microorganism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108841766A true CN108841766A (en) | 2018-11-20 |
Family
ID=64195746
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810840924.0A Pending CN108841766A (en) | 2018-07-27 | 2018-07-27 | A kind of preparation method of the fresh-keeping bacteria strain of microorganism |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108841766A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102851244A (en) * | 2012-09-11 | 2013-01-02 | 海南大学 | Bacillus used for controlling banana crown rot, and application thereof |
| CN103274834A (en) * | 2013-06-09 | 2013-09-04 | 苏州仁成生物科技有限公司 | Special insecticidal sterilizing microbial fertilizer for garlic and preparation method thereof |
| CN103304329A (en) * | 2013-06-27 | 2013-09-18 | 苏州仁成生物科技有限公司 | Special organic microbial fertilizer for preventing and treating soft rot of Chinese cabbages |
| CN103431027A (en) * | 2013-08-08 | 2013-12-11 | 河南科技大学 | Fresh-keeping method for prolonging preservation duration of strawberries |
| CN105837317A (en) * | 2016-03-24 | 2016-08-10 | 胡从玉 | Planting method of inter-planting pineapples in garlic ridge farmland |
-
2018
- 2018-07-27 CN CN201810840924.0A patent/CN108841766A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102851244A (en) * | 2012-09-11 | 2013-01-02 | 海南大学 | Bacillus used for controlling banana crown rot, and application thereof |
| CN103274834A (en) * | 2013-06-09 | 2013-09-04 | 苏州仁成生物科技有限公司 | Special insecticidal sterilizing microbial fertilizer for garlic and preparation method thereof |
| CN103304329A (en) * | 2013-06-27 | 2013-09-18 | 苏州仁成生物科技有限公司 | Special organic microbial fertilizer for preventing and treating soft rot of Chinese cabbages |
| CN103431027A (en) * | 2013-08-08 | 2013-12-11 | 河南科技大学 | Fresh-keeping method for prolonging preservation duration of strawberries |
| CN105837317A (en) * | 2016-03-24 | 2016-08-10 | 胡从玉 | Planting method of inter-planting pineapples in garlic ridge farmland |
Non-Patent Citations (7)
| Title |
|---|
| 刘娟: "D-乳酸生产菌的诱变育种及发酵条件优化", 《中国优秀博硕士学位论文全文数据库(硕士)基础科学辑》 * |
| 刘贯锋: "芽胞杆菌分类学特征及其生防功能菌株筛选", 《中国优秀博硕士学位论文全文数据库(硕士)农业科技辑》 * |
| 李建颖等: "《食品添加剂速查手册》", 30 November 2017, 南开大学出版社 * |
| 沈兆敏: "《中国果树实用新技术大全 常绿果树卷》", 31 January 1999, 中国农业科技出版社 * |
| 王刚等: "玉米秸秆同步糖化生物炼制 D-乳酸的工艺研究", 《吉林农业大学学报》 * |
| 邓林等: "苯乳酸菌发酵液在牛肉保鲜中的应用研究", 《食品发酵科技》 * |
| 钟业俊: "复合保鲜剂（OAA-7）抑菌特性及其在水果保鲜中的应用研究", 《中国优秀博硕士学位论文全文数据库(硕士)工程科技Ⅰ辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102586155A (en) | Lactobacillus plantarum N13 and use thereof | |
| CN106172507B (en) | Applications of the bacillus subtilis HMB19198 in prevention powdery mildew of cucumber | |
| CN103013861B (en) | Preparation method of bacillus subtilis HJDA32 and bacteriocin generated by bacillus subtilis HJDA32 | |
| CN105439723A (en) | Bacillus amyloliquefaciens insecticide-fertilizer for farm onsite fermentation and applications thereof | |
| CN105132312A (en) | Bacillus subtilis and application thereof as well as microbial fungicide containing bacillus subtilis and preparation method of microbial fungicide | |
| CN107660557B (en) | Bactericidal composition containing bacillus amyloliquefaciens and difenoconazole | |
| CN110387340B (en) | Lactobacillus plantarum L16 and application thereof in preventing and treating vegetable diseases | |
| CN105439725A (en) | Paenibacillus polymyxa pesticide-fertilizer for farm onsite fermentation and applications thereof | |
| CN104397637A (en) | Method for preparing pickled guncus via fermentation of direct vat composite lactic acid bacteria | |
| CN105123763A (en) | Applications of bacillus subtilis fermentation broth, microbial fungicide containing bacillus subtilis, and preparation method of microbial fungicide | |
| CN110432294A (en) | A kind of bacteriostatic agent of biofermentation, preparation method and application | |
| CN108112445A (en) | Vivo dish | |
| CN107522567A (en) | A kind of preparation method of apple special bio fertilizer | |
| CN104705611A (en) | Processing method of fermented lotus sprouts | |
| CN108990693A (en) | A kind of mushroom crossbreeding and breeding method | |
| CN108373978A (en) | A kind of microorganism formulation and its application | |
| CN105831162B (en) | A kind of shoot vegetable seed soaking microbial inoculum and its application | |
| CN107087640B (en) | Bactericidal composition containing bacillus amyloliquefaciens and prochloraz and application thereof | |
| CN108841766A (en) | A kind of preparation method of the fresh-keeping bacteria strain of microorganism | |
| CN109258305A (en) | A kind of method for promoting sclerotium of morchella esculenta to be formed and its fluid nutrient medium used | |
| CN109122024A (en) | A kind of method of the efficient bag cultivating of Pleurotus eryngii | |
| CN105502679B (en) | The preparation method of high-activity ocean yeast dry powder | |
| CN108283188A (en) | A kind of microbial bacterial agent and its preparation method and application for preventing ginseng rust | |
| CN104628434B (en) | A kind of preparation method of the bio-fertilizer of anti-black fruit fructus lycii pathology fungi | |
| CN104642018B (en) | A kind of method of the disease-resistant growth-promoting of Pericarpium Zanthoxyli Biological control |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181120 |
|
| RJ01 | Rejection of invention patent application after publication |