CN108796134B - 一种***检测探针序列及其应用 - Google Patents

Abstract

本发明涉及基因工程技术领域，公开了一种***检测探针序列及其应用。本发明所述探针序列包括高危型HPV16、18、31、33、35、39、45、51、52、56、58、59、66和68型中的一种或两种以上的探针序列；所述各高危型HPV探针序列均由捕获延伸探针、K型标记延伸探针和封闭探针三类探针序列组成。本发明针对多种高危型人***瘤病毒型E6E7区域进行延伸捕获探针、K型标记探针以及封闭探针的设计和人为优化，进一步提高了基于类似bDNA和QuantiMAT技术对***细胞检测的特异性，表现出较佳的定量限、检测限以及灵敏度，并可应用于此类试剂盒的制备中。

Description

一种***检测探针序列及其应用

技术领域

本发明涉及基因工程技术领域，具体涉及一种***检测探针序列及其应用。

背景技术

人***状瘤病毒(HPV)与***关系密切，***是妇女第二大致死癌病，临床证明95-99.7％的***病例和高危型HPV病毒感染有关。自1977年Laverty在电镜中观察到***活检组织中存在人***状瘤病毒(HPV)颗粒，以及Zur Hausen提出HPV病毒与***发病相关联的假设后，国内外学者对此问题进行了大量的研究，最终在1995年的IARC专题讨论会上通过HPV感染是***的主要原因，即HPV感染是***发生的先决必要引发条件。

HPV可分为高危型和低危型两种，长期持续感染高危型可以致癌，危害生命。低危型HPV可导致生殖器***，影响生活质量。同时，有一定比例的低危型感染还伴有高危型混合感染。因此，临床***患者进行高危及低危HPV病毒检测非常重要。高危型HPV导致癌病的机会较高，科学研究发现至少有20种高危型HPV与95％的早期浸润和浸润性子***有关。HPV感染，特别是高危型HPV感染，与***的发生存在明确关系。从近期在欧洲及其它各地的人群随意HPV筛选结果证实，HPV可以在99.8％的***患者中发现。

E6/E7是致癌基因，编码病毒癌蛋白，在细胞转化和维持转化组织恶性表型的过程中起着至关重要的作用。宫颈病变直至***的发展过程与HPV基因组整合至宿主细胞基因组密切相关，伴随着E2基因的丢失和随后E6/E7致癌基因的过度表达。E6/E7基因编码的致癌蛋白是导致宫颈上皮癌变的重要因子，这两种蛋白与细胞内两种主要抑癌蛋白p53和pRb的结合以及调控可以明显改变细胞生长周期和DNA修复，由此导致基因组的不稳定性是细胞转化和永生化的必要条件。

从低级别病变发展为癌的过程中，HPV基因组会发生整合。在整合过程中，E6/E7基因持续存在，L1基因可能会丢失。但由于L1基因相对保守，更适合于以通用引物为靶点的检测，由于受限于现有的技术平台，几乎所有的HPV检测产品均以L1为检测靶点。在宫颈高级别病变中，由于基因组的整合会使L1基因有丢失的可能，因此，以L1基因作检测靶点会对HPV有漏检的可能。相反，如果检测靶点设计在E6/E7基因，不管HPV基因组是否发生整合，都可以检测出HPV阳性的病人。

现有专利CN105861669A公开了一种快速捕获杂交靶标物分支链DNA信号放大的检测方法(QuantiMAT技术)，其灵敏度和耗时均优于bDNA技术。在其实施例中提供了检测高危型人***瘤病毒(HPV)16型E6E7区域的相关试验，但是不同的QuantiMAT探针序列对于高危型HPV的特异性有着最为直接的影响，依照ProbeDesigner V1.0工具设计出的QuantiMAT探针序列，在检测高危型HPV时并不能保证具备较高的特异性，致使定量限(LOQ)、检测限(LOD)以及灵敏度受到影响。

发明内容

有鉴于此，本发明的目的在于提供一种***检测探针序列及其应用，使得所述探针序列能够检测高危型HPV16\18\31\33\35\39\45\51\52\56\58\59\66\68型，并具备较高的特异性，可应用于***检测相关应用中。

为实现上述发明目的，本发明提供如下技术方案：

一种***检测探针序列，包括高危型HPV16、18、31、33、35、39、45、51、52、56、58、59、66和68型中的一种或两种以上的探针序列；所述各高危型HPV探针序列均由捕获延伸探针、K型标记延伸探针和封闭探针三类探针序列组成；

其中，所述高危型HPV31型捕获延伸探针序列如SEQ ID No.1-6所示，所述高危型HPV31型K型标记延伸探针如SEQ ID No.7-20所示，所述高危型HPV31型封闭探针如SEQ IDNo.21-27所示；

所述高危型HPV33型捕获延伸探针序列如SEQ ID No.28-33所示，所述高危型HPV33型K型标记延伸探针如SEQ ID No.34-49所示，所述高危型HPV33型封闭探针如SEQ IDNo.50-55所示；

所述高危型HPV35型捕获延伸探针序列如SEQ ID No.56-62所示，所述高危型HPV35型K型标记延伸探针如SEQ ID No.63-78所示，所述高危型HPV35型封闭探针如SEQ IDNo.79-82所示；

所述高危型HPV39型捕获延伸探针序列如SEQ ID No.83-90所示，所述高危型HPV39型K型标记延伸探针如SEQ ID No.91-112所示，所述高危型HPV39型封闭探针如SEQID No.113所示；

所述高危型HPV45型捕获延伸探针序列如SEQ ID No.114-121所示，所述高危型HPV45型K型标记延伸探针如SEQ ID No.122-139所示，所述高危型HPV45型封闭探针如SEQID No.140-146所示；

所述高危型HPV51型捕获延伸探针序列如SEQ ID No.147-153所示，所述高危型HPV51型K型标记延伸探针如SEQ ID No.154-173所示，所述高危型HPV51型封闭探针如SEQID No.174-175所示；

所述高危型HPV52型捕获延伸探针序列如SEQ ID No.176-178所示，所述高危型HPV52型K型标记延伸探针如SEQ ID No.179-194所示，所述高危型HPV52型封闭探针如SEQID No.195-204所示；

所述高危型HPV56型捕获延伸探针序列如SEQ ID No.205-208所示，所述高危型HPV56型K型标记延伸探针如SEQ ID No.209-228所示，所述高危型HPV56型封闭探针如SEQID No.229-234所示；

所述高危型HPV58型捕获延伸探针序列如SEQ ID No.235-238所示，所述高危型HPV58型K型标记延伸探针如SEQ ID No.239-255所示，所述高危型HPV58型封闭探针如SEQID No.256-262所示；

所述高危型HPV59型捕获延伸探针序列如SEQ ID No.263-267所示，所述高危型HPV59型K型标记延伸探针如SEQ ID No.268-289所示，所述高危型HPV59型封闭探针如SEQID No.290-292所示；

所述高危型HPV66型捕获延伸探针序列如SEQ ID No.293-298所示，所述高危型HPV66型K型标记延伸探针如SEQ ID No.299-316所示，所述高危型HPV66型封闭探针如SEQID No.317-321所示；

所述高危型HPV68型捕获延伸探针序列如SEQ ID No.322-329所示，所述高危型HPV68型K型标记延伸探针如SEQ ID No.330-349所示，所述高危型HPV68型封闭探针如SEQID No.350-352所示；

所述高危型HPV18型捕获延伸探针序列如SEQ ID No.353-357所示，所述高危型HPV18型K型标记延伸探针如SEQ ID No.358-379所示，所述高危型HPV18型封闭探针如SEQID No.380-383所示；

所述高危型HPV16型捕获延伸探针序列如SEQ ID No.384-390所示，所述高危型HPV16型K型标记延伸探针如SEQ ID No.391-410所示，所述高危型HPV16型封闭探针如SEQID No.411-412所示。

针对类似于bDNA与QuantiMAT技术中相关探针在检测***时特异性不佳的问题，本发明提供了一组经过人为优化后的高特异性探针，能够在定量限(LOQ)、检测限(LOD)以及灵敏度方面具备较优异的效果，同时检测信号和阳性细胞之间的线性关系也较优。由此，本发明提供了所述探针序列在制备检测***试剂盒中的应用。其中，所属***包括高危型HPV16\18\31\33\35\39\45\51\52\56\58\59\66\68型中的一种或多种。

根据上述应用，本发明提供了一种检测***的试剂盒，包括本发明所述探针序列。

更为具体地，所述试剂盒还包括信号放大前体、信号放大体、碱性磷酸酶(AP)标记的报告探针、化学发光底物以及偶联有通用探针的载体中的一种或两种以上；

其中，所述通用探针与本发明所述捕获延伸探针的部分序列互补配对；

所述信号放大前体包括放大前体前置引导区序列和多次重复的放大体前置引导区互补序列；

所述信号放大体包括放大体前置引导区序列和多次重复的报告探针互补序列；

所述放大前体前置引导区序列与本发明所述K型标记延伸探针部分序列互补配对；

所述放大体前置引导区序列与所述多次重复的放大体前置引导区互补序列互补配对；

所述碱性磷酸酶标记的报告探针与所述多次重复的报告探针重复区互补序列互补配对。

根据本领域经常使用的各种载体形式，本发明所述载体优选为磁珠或细胞培养板，通用探针可通过一些修饰，比如氨基修饰既可以与磁珠表面的基团相偶联，也可通过包被液包被在细胞培养板上。

在本发明具体实施方式中，本发明提供了一种具体的通用探针，其序列如SEQ IDNo.413所示。

在发光底物的选择中，本发明选择金刚烷衍生物作为AP报告探针的发光底物，用于化学发光分析仪检测。

本发明所述信号放大前体、信号放大体、碱性磷酸酶(AP)标记的报告探针可对应参考专利CN105861669A中公开的初级信号放大链、次级信号放大链和AP偶联的报告探针中的描述或具体的序列设计。

作为优选，本发明所述多次重复为20次重复。

作为优选，所述通用探针与本发明所述捕获延伸探针的部分序列互补配对，其中所述部分序列为本发明所述捕获延伸探针的3’端至5’方向的16bp序列；在本发明具体实施方式中，所有末端16bp序列为CTCTTGGAAAGAAAGT的探针都属于捕获延伸探针，其能够和SEQID No.413所示的通用探针互补配对结合。

作为优选，所述放大前体前置引导区序列与本发明所述K型标记延伸探针部分序列互补配对，其中所述部分序列为本发明所述K型标记延伸探针的3’端至5’方向的14bp序列；在本发明具体实施方式中，所有末端14bp序列为ctgagtcaaagcat或gaagttaccgtttt的探针都属于K型标记延伸探针，其能够和所述放大前体前置引导区序列互补配对结合，由此也可以设计出所述放大前体前置引导区序列。

通过ProbeDesigner V1.0工具(http://www.probedesigner.cn/)设计出一套针对高危型HPV16\18\31\33\35\39\45\51\52\56\58\59\66\68型的QuantiMAT探针序列作为对照，为保证可对比性，对照探针中所有末端16bp序列为CTCTTGGAAAGAAAGT的探针都属于捕获延伸探针(CE)，所有末端14bp序列为ctgagtcaaagcat或gaagttaccgtttt的探针都属于K型标记延伸探针(LE)，其余为封闭探针(BL)，具体如下：

HPV31型：

NKV31001-CE：GTCTTTCTGCAGGATTTTTGAACTTTTTCTCTTGGAAAGAAAGT；NKV31002-CE：GCTTAGTTCATGCAATTTCCGAGTTTTTCTCTTGGAAAGAAAGT；NKV31003-CE：CCTCTGTTTCTGTTAACTGACCTTTGTTTTTCTCTTGGAAAGAAAGT；NKV31004-CE：ATCTAAATTCACTTACTTTTGAATAAAATTTTTTCTCTTGGAAAGAAAGT；NKV31005-CE：CATATACCTTTGTTTGTCAATTTTTCTTTTTTCTCTTGGAAAGAAAGT；NKV31006-CE：ACGCATGTTTACACTTGGGTTTTTTTTCTCTTGGAAAGAAAGT；NKV31007-CE：TCTGAGCTGTCGGGTAATTGCTTTTTCTCTTGGAAAGAAAGT；NKV31008-LE：GTAGGGTATTTCCAATGCCGATTTTTGAAGTTACCGTTTT；NKV31009-LE：CAGTAGACACAATTCAATCTTAGTTCATCTTTTTCTGAGTCAAAGCAT；NKV31010-LE：GTGGTGTGTCGTCCCTATATACTATTTTTTTGAAGTTACCGTTTT；NKV31011-LE：CTTAAACATTTTGTACACACTCCGTTTTTTCTGAGTCAAAGCAT；NKV31012-LE：ACACGTTATACACCTAATTAACAAATCATTTTTGAAGTTACCGTTTT；NKV31013-LE：TCTTCTGGACACAACGGTCTTTGTTTTTCTGAGTCAAAGCAT；NKV31014-LE：TCTTTTTATCCAAATGTCTTTGTTTTTTTTTGAAGTTACCGTTTT；NKV31015-LE：TTCCTCCTATGTTGTGGAATCGTTTTTTTCTGAGTCAAAGCAT；NKV31016-LE：CTTGCAACGTAGGTGTTTCTCCTTTTTGAAGTTACCGTTTT；NKV31017-LE：CTCAGGTTGCAAATCTAACACATAGTTTTTTCTGAGTCAAAGCAT；NKV31018-LE：GGACTGTCTATGACATCCTCCTCATTTTTGAAGTTACCGTTTT；NKV31019-LE：CCGGTTCTGCTTGTCCAGCTTTTTTCTGAGTCAAAGCAT；NKV31020-BL：GTTAAATCTGTAAATGCAAAATCTAATA；NKV31021-BL：AATGTTGTTCCATACACACTATATCTATACC；NKV31022-BL：CTATGCAACGTCCTGTCCACC；NKV31023-BL：CAGTACGAGGTCTTCTCCAACATG；NKV31024-BL：TCATAACAGTGGAGGTCAGTTGC；

HPV39型：

NKV39001-CE：TACCTCGGTTTGCTGTAGTGGTTTTTCTCTTGGAAAGAAAGT；NKV39002-CE：GGTTCCCCGTCCCTATATACTATTTTTCTCTTGGAAAGAAAGT；NKV39003-CE：TTTATGAAATCTTCGTTTGCTATTTTTTTTCTCTTGGAAAGAAAGT；NKV39004-CE：GGTCCACGCATATCTGATGTTATATTTTTCTCTTGGAAAGAAAGT；NKV39005-CE：TTTCCTGCAAGGTGGGCTTTTTTTTCTCTTGGAAAGAAAGT；NKV39006-CE：CCGTGAGGCTTCTACTACCAGCTTTTTTCTCTTGGAAAGAAAGT；NKV39007-CE：ACAAATCCTAGTGAGTCCATAAACAGTTTTTCTCTTGGAAAGAAAGT；NKV39008-LE：CATGATTGGCATGCAGCTAGTTTTTTGAAGTTACCGTTTT；NKV39009-LE：CCCGTATTTTAGCATAAAATTTTATATTTTTCTGAGTCAAAGCAT；NKV39010-LE：CCGAGTCCGAGTAATATCGTAGCTTTTTTGAAGTTACCGTTTT；NKV39011-LE：TTAGTTATATTTTCTAATGTAGTTGCATACATTTTTCTGAGTCAAAGCAT；NKV39012-LE：CACAGCGGTTTCAGACAACACATTTTTGAAGTTACCGTTTT；NKV39013-LE：AGGTGTCTTAATTTTTCTGCTGGATTTTTCTGAGTCAAAGCAT；NKV39014-LE：TTCATTGTAAGGACATAAATCTAATACAATTTTTGAAGTTACCGTTTT；NKV39015-LE：CATACAAGGTCAACCGGCTGTATTTTTTCTGAGTCAAAGCAT；NKV39016-LE：GTCGGGTTCATCTATTTCATCCTTTTTTGAAGTTACCGTTTT；NKV39017-LE：TTGATGTTGGTGATTAACTGCATGTTTTTCTGAGTCAAAGCAT；NKV39018-LE：GGTTCATCCCGTCTGGCTAGTAGTTTTTGAAGTTACCGTTTT；NKV39019-LE：GAACACTGTATTGTGTGACGCTGTTTTTTCTGAGTCAAAGCAT；NKV39020-BL：CATATAAATCACTAAATGCAAATTCATA；NKV39021-BL：TGCACCTTATTAATAAATTATATAACTTTGTA；NKV39022-BL：ACTGTCCTGTATAGCTTCCTGCTAT；NKV39023-BL：TGGTCCAGCACCGTCGAC；NKV39024-BL：TGCGGTCCTCCCGTTTTG；NKV39025-BL：CTTGGGTTTCTCTTCGTGTTAGTC；NKV39026-BL：CTGACTCTCCTAATTGCTCGTGA；NKV39027-BL：GCAGTGTGTTGTTACACTTACAACAC；NKV39028-BL：CTGCTGTAGTTGTCGCAGAGTATC；

HPV33型：

NKV333-BL：CAGATCGTTGCAAAGGTTTTTT；NKV338-BL：ATTTCCATATACAGAATAATTATAATGTCTAT；NKV339-BL：AAGGTTTTTTAACTGTTTGTTCTAATGT；NKV3310-BL：GACATATAATACACCTAATTAATATTTCATTTA；NKV3314-BL：CAGCGCCCTGCCCAAC；NKV3320-BL：CTCATAGCAGTATAGGTCAGTTGGTT；NKV3321-BL：CCTCATCTGAGCTGTCACTTAATTG；NKV3327-BL：TTGCTGTATGGTTCGTAGGTCAC；NKV334-CE：CTGCAAATGCAAAATCATATACCTTTTTTCTCTTGGAAAGAAAGT；NKV337-CE：ATTCACTAATTTTAGATAAGAACCGCTTTTTCTCTTGGAAAGAAAGT；NKV3313-CE：GACCCGAAATATTATGAAATCGTTTTTTTCTCTTGGAAAGAAAGT；NKV3315-CE：ACCTCCAACACGCCGCATTTTTCTCTTGGAAAGAAAGT；NKV3322-CE：GCCGGTCCAAGCCTTCATTTTTTCTCTTGGAAAGAAAGT；NKV3328-CE：CAATATTCACTGTGCCCATAAGTAGTTTTTCTCTTGGAAAGAAAGT；NKV331-LE：TGTTGTGTATAGTTGTCTCCAATGCTTTTTTGAAGTTACCGTTTT；NKV332-LE：GCATTCCACGCACTGTAGTTCAATTTTTCTGAGTCAAAGCAT；NKV335-LE：ATTTCCCTCTCTATATACAACTGTTAAATTTTTTGAAGTTACCGTTTT；NKV336-LE：AAACACAGTTTACATATTCCAAATGGTTTTTCTGAGTCAAAGCAT；NKV3311-LE：TTTCTTGAGGACACAAAGGTCTTTTTTTTGAAGTTACCGTTTT；NKV3312-LE：TGTTTAAATCCACATGTCGTTTTTTTTTTCTGAGTCAAAGCAT；NKV3316-LE：GTGCAGTTTCTCTACGTCGGGTTTTTGAAGTTACCGTTTT；NKV3317-LE：ATGGCGTTTTTACACGTCACATTTTTCTGAGTCAAAGCAT；NKV3318-LE：AACGTTGGCTTGTGTCCTCTCTTTTTGAAGTTACCGTTTT；NKV3319-LE：CAGGATATAAATCTAAAACATATTCCTTTTTTTTCTGAGTCAAAGCAT；NKV3323-LE：GCTGGTTGTGCTTGTCCATCTGTTTTTGAAGTTACCGTTTT；NKV3324-LE：CAGGTTACAATGTAGTAATCAGCTGTGTTTTTCTGAGTCAAAGCAT；NKV3325-LE：AACTGTGGTGTTACAAGTGTGACAATTTTTGAAGTTACCGTTTT；NKV3326-LE：TTGCTGTACTGTTGACACATAAACGTTTTTCTGAGTCAAAGCAT；

HPV35型：

NKV356-BL：TCTATATACTATACACAAATCATAGCATGC；NKV358-BL：GAATAAAATTTTAAACATTTCATGCA；NKV359-BL：ACTATATCTATACCATCTATATTCACTTATTTTT；NKV3513-BL：TTGCTTTTCAACTGGACACAGC；NKV3514-BL：GAATCGTTTTTTTTCTTCTAAATGTCT；NKV3516-BL：AACAGGACATACACCGACCTGTC；NKV3517-BL：GGTTTCTCTACGTGTTGGTTTCC；NKV3518-BL：TTCTCCATGCATGATTACACCTC；NKV3526-BL：CAGACGTAGTGTCGCCTCACAT；NKV3527-BL：TGTCAATGTGTGTGCTCTGTACACA；NKV353-CE：CAAACAAATTTCATGGATGCTTTCTTTTTCTCTTGGAAAGAAAGT；NKV357-CE：TACTCCATATGGCTGGCCTTCTTTTTCTCTTGGAAAGAAAGT；NKV3510-CE：TGTTTTTCTAACGTTTCTCCATACACTTTTTCTCTTGGAAAGAAAGT；NKV3515-CE：CACCGTCCACCGATGTTATGTTTTTCTCTTGGAAAGAAAGT；NKV3523-CE：TCCAGCTGGACCGTCAATAGTATTTTTTCTCTTGGAAAGAAAGT；NKV3528-CE：CCATTAATAAATCTTCCAATTTACGTATTTTTCTCTTGGAAAGAAAGT；NKV351-LE：GCAGTTTGTAAGGTCGTTCAGCTTTTTTGAAGTTACCGTTTT；NKV352-LE：TTCTACCTCGTTGCACAAATCATTTTTTCTGAGTCAAAGCAT；NKV354-LE：TAATTCTTGTTTGCAGTATACACAATTTTTTTGAAGTTACCGTTTT；NKV355-LE：AAAGTCATATACCTCACTCCGCTGTTTTTCTGAGTCAAAGCAT；NKV3511-LE：AATAAATGACATAACTGTTTGTTGCATTTTTTGAAGTTACCGTTTT；NKV3512-LE：GGTTTTTGACATGTAATACACCTAATTTTTTTCTGAGTCAAAGCAT；NKV3519-LE：TCTAAAACATAGTCTTGCAATGTAGTTATTTTTTGAAGTTACCGTTTT；NKV3520-LE：AGTTGCCTCGGGTTCCAAATTTTTCTGAGTCAAAGCAT；NKV3521-LE：ACACAATTGCTCATAACAGTATAGGTCTTTTTGAAGTTACCGTTTT；NKV3522-LE：CTTCCTCCTCCTCTGAGCTGTCTTTTTCTGAGTCAAAGCAT；NKV3524-LE：GGAGGTGTCTGGTTTTGCTTGTTTTTGAAGTTACCGTTTT；NKV3525-LE：TTACAACAGGACGTTACAATATTATAATTTTTTTCTGAGTCAAAGCAT；

HPV45型：

NKV45E6E75-BL：GCAGCATATGCTATACAGTCTCTATACAC；NKV45E6E76-BL：GGAATAAAAGTCTATACATTTATGGCAT；NKV45E6E77-BL：GAGTTTGAATAATATCTTAATTCTCTAATTCT；NKV45E6E78-BL：TTTTTTCCAGTGTCTCTCCATATACA；NKV45E6E79-BL：CTTATTAACAAATTATACAACTCTGTATTAGTTA；NKV45E6E710-BL：TCTGGCACCGCAGGCAC；NKV45E6E713-BL：TCCAGCTATGCTGTGGAATCTT；NKV45E6E726-BL：TTACAACATACACACAAAATTTTGTGA；NKV45E6E73-CE：TATACCTCTGTGCGTTCCAATGTTTTTTCTCTTGGAAAGAAAGT；NKV45E6E74-CE：TATAAATAAATCTTTAAAAGCAAATTGATTTTTCTCTTGGAAAGAAAGT；NKV45E6E718-CE：CAGTGTTGCTCGGGGTCCATTTTTCTCTTGGAAAGAAAGT；NKV45E6E723-CE：TGACTAACGCCATCTGCTTCATTTTTTCTCTTGGAAAGAAAGT；NKV45E6E727-CE：AGCTCAATTCTGCCGTCACACTTTTTCTCTTGGAAAGAAAGT；NKV45E6E728-CE：CATCTGCCGAGCTCTCTACTGTATTTTTCTCTTGGAAAGAAAGT；NKV45E6E711-LE：TTTCTGCTGGGTTCAATGGTTTTTTTGAAGTTACCGTTTT；NKV45E6E712-LE：CGTTTGTCCTTAAGGTGTCTACGTTTTTTTCTGAGTCAAAGCAT；NKV45E6E714-LE：TGTATTACACTGCCCTCGGTACTGTTTTTGAAGTTACCGTTTT；NKV45E6E715-LE：GCCGTGCCTGGTCACAACATTTTTCTGAGTCAAAGCAT；NKV45E6E716-LE：CCTACGTCTGCGAAGTCTTTCTTTTTTTGAAGTTACCGTTTT；NKV45E6E717-LE：TGCATACTTATTGCTATACTTGTGTTTCTTTTTCTGAGTCAAAGCAT；NKV45E6E719-LE：TTCCAAATGCAATACAATTTCTTGTTTTTGAAGTTACCGTTTT；NKV45E6E720-LE：CAACAGGATCTAATTCATTCTGAGGTTTTTCTGAGTCAAAGCAT；NKV45E6E721-LE：TTAATTGCTCGTAACACAACAGGTTTTTTGAAGTTACCGTTTT；NKV45E6E722-LE：CGTTTTCCTCCTCTGACTCGCTTTTTCTGAGTCAAAGCAT；NKV45E6E724-LE：TCGGGCTGGTAGTTGTGCATTTTTGAAGTTACCGTTTT；NKV45E6E725-LE：CGCTGTGGTTCGGCTCGTTTTTCTGAGTCAAAGCAT；

HPV52型：

NKV526-BL：CTATTCGTAAATCTGTAAATAGAAACTTG；NKV527-BL：CGCCATATGGATTATTGTCTCTATATA；NKV528-BL：AAAAGCGTAGGCACATAATACACA；NKV529-BL：TGATAATGCCTATATTCACTTATCTTAGATA；NKV5210-BL：TCTAATGTTTTCCCATACAGTGAATAT；NKV5211-BL：CACTTAATGGTTTTTTTACCCTCTCT；NKV5212-BL：CGTTTGACAAATTATACATCTAATAGTTATTT；NKV5215-BL：CCAACGACCCATAATATTATGAAA；NKV5220-BL：TTGTTTCAGGTTGCAGATCTAATATAT；NKV5221-BL：AATTGCTCATAGCAGTGTAGGTCAG；NKV5222-BL：CCTCCTCATCTGAGCTGTCACCT；NKV5228-BL：TGTAGAGTACGAAGGTCCGTCG；NKV5230-BL：CCGGGGCACACAACTTGTAA；NKV5231-BL：GGTTGTTTATAGCCGTGCACAG；NKV521-CE：GTGCAGGGTCCGGGGTCTTTTTCTCTTGGAAAGAAAGT；NKV5216-CE：CACTCTGAACAGCGCCCTGTTTTTTCTCTTGGAAAGAAAGT；NKV5217-CE：CACAGGTCGGGGTCTCCAATTTTTCTCTTGGAAAGAAAGT；NKV5227-CE：CAGTGCTATGAATGCATAGCCGTTTTTCTCTTGGAAAGAAAGT；NKV5229-CE：TGTGCCCAACAGCATTTGCTTTTTCTCTTGGAAAGAAAGT；NKV5232-CE：TTCAGGGTCCTCCATTGCAGTTTTTCTCTTGGAAAGAAAGT；NKV522-LE：CTTCCAGCACCTCACACAATTCTTTTTGAAGTTACCGTTTT；NKV523-LE：GCCTTATTTCATGCACCGATTTTTTTCTGAGTCAAAGCAT；NKV524-LE：TTTGCACTGCACACACTGCATTTTTGAAGTTACCGTTTT；NKV525-LE：TATACCTCTCTTCGTTGTAGCTCTTTTTTTTCTGAGTCAAAGCAT；NKV5213-LE：TTTCTTTTTCTTCAGGACATAATGGTTTTTGAAGTTACCGTTTT；NKV5214-LE：TCGCTTGTTTGCATTAACATGTCTTTTTCTGAGTCAAAGCAT；NKV5218-LE：CGCATGACGTTACACTTGGGTTTTTTGAAGTTACCGTTTT；NKV5219-LE：AATCTTTTATAGTTGCTTTGTCTCCATTTTTCTGAGTCAAAGCAT；NKV5223-LE：GCCGGTCCACACCATCTGTATTTTTTGAAGTTACCGTTTT；NKV5224-LE：GCTTGTTCTGCTTGTCCATCTGTTTTTCTGAGTCAAAGCAT；NKV5225-LE：ATGTCACAATGTAGTAATTGCTTGTGTTTTTGAAGTTACCGTTTT；NKV5226-LE：TAGTGTGCTATCACAACTGTGACAATTTTTTCTGAGTCAAAGCAT；

HPV51型：

NKV51001-CE：GGTCTTTCCCTCTTGTCTTCGAATTTTTCTCTTGGAAAGAAAGT；NKV51002-CE：TGCATAGAAACGTTCAAAGCTTCTTTTTCTCTTGGAAAGAAAGT；NKV51003-CE：TTTGAATAAAACAGTAAACATTGTTTGTTTTTCTCTTGGAAAGAAAGT；NKV51004-CE：TCGATAAATCATATAAGCTTTTTTTAGTAATTTTTCTCTTGGAAAGAAAGT；NKV51005-CE：CGCATTGCCCCGTCCAATTTTTCTCTTGGAAAGAAAGT；NKV51006-CE：CGTGTACGTTGCCAGCAATTAGTTTTTCTCTTGGAAAGAAAGT；NKV51007-CE：GGAGCTTCAATTCTGTAACACGTATTTTTCTCTTGGAAAGAAAGT；NKV51008-LE：TTACAATACACACACACTACCTGTATATTGTTTTTGAAGTTACCGTTTT；NKV51009-LE：TTATATACATCTGCTCTACATAATTCCTTTTTTTTCTGAGTCAAAGCAT；NKV51010-LE：ATACAATCTTAATTTCAGTAAATGCTACATTTTTGAAGTTACCGTTTT；NKV51011-LE：CATACTGCATATGGATTATTATCCCTATTTTTTCTGAGTCAAAGCAT；NKV51012-LE：ACCTGCTATAACGTCTATACTCTCTAATTTTTTTGAAGTTACCGTTTT；NKV51013-LE：TTGCCTCTAATGTAGTACCATACACAGTTTTTCTGAGTCAAAGCAT；NKV51014-LE：GTGGTCTTTGACATCTATGACACCTTATTTTTGAAGTTACCGTTTT；NKV51015-LE：TTTGCTTTTCTTCAGGCCCAATTTTTCTGAGTCAAAGCAT；NKV51016-LE：CACTTGGGTTTCGTTACGTTGTTTTTTGAAGTTACCGTTTT；NKV51017-LE：CATTACCACGCATGGCTTTATTATTTTTCTGAGTCAAAGCAT；NKV51018-LE：TGCAATACTACATCTTTTAATTGTGGTATTTTTGAAGTTACCGTTTT；NKV51019-LE：AGTCAATTTCAGTCTGTGGTGTTAAATTTTTCTGAGTCAAAGCAT；NKV51020-LE：TCAAATTGCTCGTAGCATTGCATTTTTGAAGTTACCGTTTT；NKV51021-LE：TACTTCATCCTCCTCCTCTGAGCTGTTTTTCTGAGTCAAAGCAT；NKV51022-BL：ACATAATTCATGCAGCGTTCGT；NKV51023-BL：CTTTTTTTTTCGTCCACCAATT；NKV51024-BL：CGTCCCGCTATTTCATGGAAC；NKV51025-BL：CTGGTAGCTGGTCACGCATATTATC；NKV51026-BL：GCCTGTCCAGCCCGTCTTT；

HPV56型：

NKV56001-CE：GTTAGTTCTTTTTTGCAATATACACATGTTTTTCTCTTGGAAAGAAAGT；NKV56002-CE：ATGCAAAATTATATACCTCAGCACGTTTTTTCTCTTGGAAAGAAAGT；NKV56003-CE：GCACACTGCATAAGGAAAATCATTTTTCTCTTGGAAAGAAAGT；NKV56004-CE：CACAATGCAATTGCTTTTCCTCTTTTTCTCTTGGAAAGAAAGT；NKV56005-CE：CTATTAGATGAAATCGTCTTTTTCTGTTTTTTCTCTTGGAAAGAAAGT；NKV56006-CE：GTCTCCAGCACCCCAAACATTTTTTCTCTTGGAAAGAAAGT；NKV56007-CE：AGGTCAATTTCTGTTTGAGGTGTTATTTTTCTCTTGGAAAGAAAGT；NKV56008-LE：CATACACTGAATAGTCATAATACCTATATTTTTTTTGAAGTTACCGTTTT；NKV56009-LE：GTTTTTTAGTTATACTTTCTAGTGTAGCTCTTTTTCTGAGTCAAAGCAT；NKV56010-LE：GTAGCACCTTATTAATAAATCACATAACTTTTTTGAAGTTACCGTTTT；NKV56011-LE：CGGAGTTAACGGACTTTGACATCTTTTTTCTGAGTCAAAGCAT；NKV56012-LE：TGTAGATTCTCTAGGTTCTCTAGATGTTTTTTTTGAAGTTACCGTTTT；NKV56013-LE：TTGGTACTTTACCATGCATGATTATACTTTTTCTGAGTCAAAGCAT；NKV56014-LE：CCTCATCCTCATCCTCTGAGCTTTTTTGAAGTTACCGTTTT；NKV56015-LE：GCTCCTGCAAATGGTCTACTTCATTTTTTCTGAGTCAAAGCAT；NKV56016-LE：CTTGTCTAGCTTGCTGTGGCCTTTTTGAAGTTACCGTTTT；NKV56017-LE：GTGTATTAGGTAACACGTATGTTGTTTAGTTTTTCTGAGTCAAAGCAT；NKV56018-LE：CACAAACTTACACTCACAACAAGGTACTTTTTGAAGTTACCGTTTT；NKV56019-LE：GGTACTGTGAATGTCCAACTGCACTTTTTCTGAGTCAAAGCAT；NKV56020-BL：TCCCTATACACTAAGTTTAATTCAGTGC；NKV56021-BL：TCTAACTTTACTATAAAACAATAAACATACTCT；NKV56022-BL：GACCCGGTCCAACCATGTG；NKV56023-BL：GTTCTAATATAACGTCTTGCAGCG；NKV56024-BL：GTCCAATTGCTCATTGCACTGT；

HPV58型：

NKV58001-CE：CTCCTCTGCGTCCTGGAACATTTTTCTCTTGGAAAGAAAGT；NKV58002-CE：TCAATTCGATTTCATGCACAGATTTTTCTCTTGGAAAGAAAGT；NKV58003-CE：GTCTTTTTGCATTCAACGCATTTTTTTCTCTTGGAAAGAAAGT；NKV58004-CE：CACTATTCTTAAATCTGCAAATACAAAGTTTTTCTCTTGGAAAGAAAGT；NKV58005-CE：AGCAATCGTAAGCACACTTTACATACTTTTTCTCTTGGAAAGAAAGT；NKV58006-CE：ATTAATATTTCATTTAAACACTTTTTTAGTGTTTTTTCTCTTGGAAAGAAAGT；NKV58007-CE：CCGTCCAAGCCTATTTCATCCTTTTTTCTCTTGGAAAGAAAGT；NKV58008-LE：ATCATGCAATGTCCGTGGTTTTTTTTGAAGTTACCGTTTT；NKV58009-LE：TGTCTCCAACGCCTGACACAATTTTTCTGAGTCAAAGCAT；NKV58010-LE：ATAATTATAATGTCTATACTCACTTATTTTAGATTTTTTGAAGTTACCGTTTT；NKV58011-LE：TTGTTCTAATGTGTCTCCATATAGCGATTTTTCTGAGTCAAAGCAT；NKV58012-LE：CAATGGTCTTTGACAAATAATACATCTATTTTTGAAGTTACCGTTTT；NKV58013-LE：TGCCTTTTTTTTTCTTGTGGACATTTTTCTGAGTCAAAGCAT；NKV58014-LE：CCTGTCCAACGACCCGAAATATTTTTTGAAGTTACCGTTTT；NKV58015-LE：TCCAACACACTGCACAGCGCTTTTTCTGAGTCAAAGCAT；NKV58016-LE：TGTGTTTGTCTACGTCGGGGTCTTTTTGAAGTTACCGTTTT；NKV58017-LE：ATGGCGTTGTTACAGGTTACACTTTTTTCTGAGTCAAAGCAT；NKV58018-LE：TCATAGCAGAATAGGTCAGTTGGTTTTTTGAAGTTACCGTTTT；NKV58019-LE：CGTCTGAGCTGTCACATAATTGCTTTTTCTGAGTCAAAGCAT；NKV58020-BL：TCATATACCTCAGATCGCTGCAAA；NKV58021-BL：TGCAAATGGATTTCCATCTCTATA；NKV58022-BL：TATGAAACCTTTTGTTTAAATCCACA；NKV58023-BL：GCGTTGGGTTGTTTCCTCTC；NKV58024-BL：TCAGGATGTAAATCTAAAATATATTCTCTTA；

HPV68型：

NKV68001-CE：GTCATGCAATGTAGTGTCCAATGTTTTTTCTCTTGGAAAGAAAGT；NKV68002-CE：TGTTAGGTGCCTTAGTTTTTCTGCTTTTTCTCTTGGAAAGAAAGT；NKV68003-CE：CGCTTACTGGTCCAGCAGTGCTTTTTCTCTTGGAAAGAAAGT；NKV68004-CE：CGGTGGGCTTTGGTCCATGTTTTTCTCTTGGAAAGAAAGT；NKV68005-CE：ATAGCTCTAACACAATTTCCTGCATTTTTCTCTTGGAAAGAAAGT；NKV68006-CE：CCCGCGACGCTTCTACTACTTTTTTCTCTTGGAAAGAAAGT；NKV68007-CE：CAAAATTTAGTGAGTCCATAAACAGCTTTTTCTCTTGGAAAGAAAGT；NKV68008-LE：CTTCTGCAATAGACACAGTCTATTGTAACTTTTTGAAGTTACCGTTTT；NKV68009-LE：CATATACCTCTGTCCGTTGTAGTTGCTTTTTCTGAGTCAAAGCAT；NKV68010-LE：ATTTAATACATGATTGGCATGCAGTTTTTGAAGTTACCGTTTT；NKV68011-LE：CGTAGTTCCCGTATTTTAGCATAAATTTTTCTGAGTCAAAGCAT；NKV68012-LE：GCATACACCGATTCCGAGTAATATTTTTTGAAGTTACCGTTTT；NKV68013-LE：CTTTGTATTAGTTATGGTTTCTAATGTAGTTTTTTTCTGAGTCAAAGCAT；NKV68014-LE：ACTCATGCACCTTATCAATAAATTATATAATTTTTGAAGTTACCGTTTT；NKV68015-LE：TGGACACAATGGTTTCAGGCATTTTTCTGAGTCAAAGCAT；NKV68016-LE：CGGCTGTATTTCATTGTATGGACTTTTTGAAGTTACCGTTTT；NKV68017-LE：TGCTCGTGACATACAAGGTCAACTTTTTCTGAGTCAAAGCAT；NKV68018-LE：CTATTTCATCGTCTGAATCTCCTAATTTTTTGAAGTTACCGTTTT；NKV68019-LE：AACTGCATGGTCGGGTTCATTTTTTCTGAGTCAAAGCAT；NKV68020-LE：GCTGTTGTTCGTCCCGTCTGTTTTTGAAGTTACCGTTTT；NKV68021-LE：CAACACAGACACTGAATTCTGTGACTTTTTCTGAGTCAAAGCAT；NKV68022-BL：CTACACATAGGTCACTAAAGGCAAATT；NKV68023-BL：CAAATGGTACCCCGTCTCTATACA；NKV68024-BL：GCTATTTTATGTAATCTTCGTTTTGT；NKV68025-BL：CGACACTGTCCTGTAAAGTTTCCT；NKV68026-BL：GTATGCGTCTGCGGTCCTCT；NKV68027-BL：CATAGTTACTTAAACTTGTGTTTCTTGAC；NKV68028-BL：GCTAGTAGTAGATGTTGGTGGTGATT；NKV68029-BL：AGTTGCAGTGCCTTGTTACACTTA；NKV68030-BL：TGTTGTAGTGTCCGCAGGTTGT；

HPV59型：

NKV592-BL：GGCAGTTTGTATGGTCGTTGTGTA；NKV593-BL：AATATTCAATGTTGTGCTCAAATCA；NKV597-BL：TACACTATAAATAAGTCATTAAAAGCAAAT；NKV598-BL：GCTGCATACGGTGTACAGTCTCTA；NKV599-BL：CATAAAATGAAATGCATTTCAGACAC；NKV5910-BL：AATCTCTATAATATCTTAATTCTCTTACTCTTG；NKV5915-BL：CTTTTTTCAGTTATATGCTTTAATTTATC；NKV5916-BL：TATATATTCCAGCTATATTATGGAATCTT；NKV5917-BL：GACACCCACGACACTGTCCTG；NKV5926-BL：GATGATTAACTCCATCTGGTTCATCT；NKV5927-BL：CAGCTCGTCTAGCTAGTAGCAAAG；NKV5928-BL：ACAATGTTGTGACGCTGTGGTT；NKV5929-BL：TTGATTATTACACTTACAACACACACAC；NKV591-CE：GGATCCTCAAAGCGTGCCATTTTTCTCTTGGAAAGAAAGT；NKV594-CE：TGATGCGAATATCATGCAGAGGTTTTTCTCTTGGAAAGAAAGT；NKV5913-CE：TCTATAACAGCGTATCAGCAGCTCTTTTTCTCTTGGAAAGAAAGT；NKV5914-CE：TGTTGGACATAGAGGTTTTAGGCATTTTTCTCTTGGAAAGAAAGT；NKV5918-CE：TAGGTGTCTTGCTCGGGTCCTTTTTCTCTTGGAAAGAAAGT；NKV5921-CE：AAAGTGTTGCTTTTGGTCCATGTTTTTCTCTTGGAAAGAAAGT；NKV595-LE：CCCCTTTGCAAAACACACAATTTTTTGAAGTTACCGTTTT；NKV596-LE：TCAAATACCTCTCTTTCTTGCAGTTTTTTTCTGAGTCAAAGCAT；NKV5911-LE：CCTCTAATGTTTCTCCATACACGGTTTTTGAAGTTACCGTTTT；NKV5912-LE：ATGTAACGGTGTCTTGGTTTCAGTTTTTCTGAGTCAAAGCAT；NKV5919-LE：GCGCTTGTCGTTGCTGTCTTTTTTGAAGTTACCGTTTT；NKV5920-LE：CATTGTTTTACACCAGTGTTTCACTACTTTTTCTGAGTCAAAGCAT；NKV5922-LE：GGTTCCAAATCTAAAACAATGTCACTTTTTGAAGTTACCGTTTT；NKV5923-LE：AAGGTCAACTTCCTCATAATTTTGTTTTTTCTGAGTCAAAGCAT；NKV5924-LE：TCAGGTAATTGCTCGTAGCACACTTTTTGAAGTTACCGTTTT；NKV5925-LE：TTTTCATTCTCGGAGTCGGAGTTTTTCTGAGTCAAAGCAT；NKV5930-LE：GCGAGGTTTCTACTACTAGCTGAAGTTTTTGAAGTTACCGTTTT；NKV5931-LE：AAGGCTCGCAATCCGTCTTTTTTTCTGAGTCAAAGCAT；

HPV16型：

NKV161-LE：GTCTGCTTTTATACTAACCGGTTTCTTTTTGAAGTTACCGTTTT；NKV162-LE：GCAGTTCTCTTTTGGTGCATAAAATTTTTTCTGAGTCAAAGCAT；NKV163-CE：CTCCTGTGGGTCCTGAAACATTTTTTTCTCTTGGAAAGAAAGT；NKV164-LE：AACTGTGGTAACTTTCTGGGTCGTTTTTGAAGTTACCGTTTT；NKV165-LE：AGTTGTTTGCAGCTCTGTGCATTTTTTCTGAGTCAAAGCAT；NKV166-BL：GCAGTACACACATTCTAATATTATATCATGTAT；NKV167-CE：CACGTCGCAGTAACTGTTGCTTTTTTTCTCTTGGAAAGAAAGT；NKV168-BL：CCCGAAAAGCAAAGTCATATACCT；NKV169-LE：TTCCCATCTCTATATACTATGCATAAATTTTTTGAAGTTACCGTTTT；NKV1610-LE：AACATTTATCACATACAGCATATGGATTTTTCTGAGTCAAAGCAT；NKV1611-BL：GTCTATACTCACTAATTTTAGAATAAAACTTTA；NKV1612-CE：TGTTGTTCCATACAAACTATAACAATAATTTTTTCTCTTGGAAAGAAAGT；NKV1613-LE：CGGTTTGTTGTATTGCTGTTCTAATTTTTGAAGTTACCGTTTT；NKV1614-LE：TAATACACCTAATTAACAAATCACACAATTTTTCTGAGTCAAAGCAT；NKV1615-LE：GGACACAGTGGCTTTTGACAGTTTTTTGAAGTTACCGTTTT；NKV1616-LE：CCAGATGTCTTTGCTTTTCTTCATTTTTCTGAGTCAAAGCAT；NKV1617-BL：TTATATTATGGAATCTTTGCTTTTTGT；NKV1618-BL：CCGGTCCACCGACCCC；NKV1619-LE：GATCTGCAACAAGACATACATCGATTTTTGAAGTTACCGTTTT；NKV1620-LE：TGGGTTTCTCTACGTGTTCTTGATTTTTTCTGAGTCAAAGCAT；NKV1621-BL：TGTATCTCCATGCATGATTACAGC；NKV1622-CE：AATCTAACATATATTCATGCAATGTAGGTTTTTCTCTTGGAAAGAAAGT；NKV1623-LE：GAGATCAGTTGTCTCTGGTTGCATTTTTGAAGTTACCGTTTT；NKV1624-LE：GCTGTCATTTAATTGCTCATAACAGTATTTTTCTGAGTCAAAGCAT；NKV1625-BL：CATCTATTTCATCCTCCTCCTCTGA；NKV1626-BL：GTTCTGCTTGTCCAGCTGGAC；NKV1627-CE：ATATTGTAATGGGCTCTGTCCGTTTTTCTCTTGGAAAGAAAGT；NKV1628-LE：TCACACTTGCAACAAAAGGTTACATTTTTGAAGTTACCGTTTT；NKV1629-LE：CGCACAACCGAAGCGTAGAGTTTTTCTGAGTCAAAGCAT；NKV1630-BL：CGAATGTCTACGTGTGTGCTTTGTA；NKV1631-CE：CCCATTAACAGGTCTTCCAAAGTATTTTTCTCTTGGAAAGAAAGT；NKV1632-BL：GGGGCACACAATTCCTAGTGTG；NKV1633-CE：GGTAGATTATGGTTTCTGAGAACAGATTTTTTCTCTTGGAAAGAAAGT；NKV1634-BL：GGTACCTGCAGGATCAGCCAT；NKV1635-CE：CCCGTACCCTCTTCCCCATTTTTTTCTCTTGGAAAGAAAGT；

HPV18型：

NKV181-CE：CGTTTTTCATTAAGGTGTCTAAGTTTTTTTTTTCTCTTGGAAAGAAAGT；NKV182-LE：CCCAGCTATGTTGTGGAATCGTTTTTTGAAGTTACCGTTTT；NKV183-LE：AATGGCACTGGCCTCTATAGTGTTTTTCTGAGTCAAAGCAT；NKV184-CE：TGCTCGGTTGCAGCACGTTTTTCTCTTGGAAAGAAAGT；NKV185-LE：TCGTTGGAGTCTTTCCTGTCGTTTTTGAAGTTACCGTTTT；NKV186-LE：CTTAATATTATACTTGTGTTTCTCTGCGTTTTTCTGAGTCAAAGCAT；NKV187-CE：TGTTGCCTTAGGTCCATGCATATTTTTCTCTTGGAAAGAAAGT；NKV188-LE：TAAATGCAATACAATGTCTTGCAATTTTTGAAGTTACCGTTTT；NKV189-LE：GGAATTTCATTTTGGGGCTCTTTTTCTGAGTCAAAGCAT；NKV1810-LE：GCTCGTGACATAGAAGGTCAACCTTTTTGAAGTTACCGTTTT；NKV1811-LE：TTTCTTCCTCTGAGTCGCTTAATTTTTTTCTGAGTCAAAGCAT；NKV1812-LE：ATGATTAACTCCATCTATTTCATCGTTTTTTGAAGTTACCGTTTT；NKV1813-LE：CGTCGGGCTGGTAAATGTTGTTTTTCTGAGTCAAAGCAT；NKV1814-BL：TGTGTGACGTTGTGGTTCAGCT；NKV1815-BL：TTCACACTTACAACACATACACAACAT；NKV1816-CE：GCTTTCTACTACTAGCTTAATTCTGGCTTTTTCTCTTGGAAAGAAAGT；NKV1817-LE：GCTCGAAGGTCGTCTGCTGATTTTTGAAGTTACCGTTTT；NKV1818-LE：TGTTCAGAAACAGCTGCTGGAATTTTTTCTGAGTCAAAGCAT；NKV1819-LE：CGGACACACAAAGGACAGGGTTTTTGAAGTTACCGTTTT；NKV1820-LE：ACTGCTGGGATGCACACCATTTTTCTGAGTCAAAGCAT；NKV1821-CE：CTGGATCAGCCATTGTTGCTTTTTTTCTCTTGGAAAGAAAGT；NKV1822-LE：CGTCAGTTTCCTCATCTGAAAACTTTTTTTGAAGTTACCGTTTT；NKV1823-LE：CGTCAAGTCATTTAAGCTTGGTACCTTTTTCTGAGTCAAAGCAT；NKV1824-CE：TACTACCTGCTAATCTTCTATGAGCTTTTTTTCTCTTGGAAAGAAAGT；NKV1825-BL：GAATTAGGAGTCAGCAGTCATTATTC；NKV1826-CE：TGGCCAGGGAAGATCCACTTTTTTCTCTTGGAAAGAAAGT；NKV1827-CE：TTGCAGCTCAATACAAAACGGTTTTTCTCTTGGAAAGAAAGT；

HPV66型：

NKV66001-CE：CCTGTGTATTGCTGAATATGGAATCTTTTTCTCTTGGAAAGAAAGT；NKV66002-CE：GGCCAATTGTTTCTATATACTAGTTTTAACTTTTTCTCTTGGAAAGAAAGT；NKV66003-CE：ATGTTGCCCCATACACTGAATATTTTTTTCTCTTGGAAAGAAAGT；NKV66004-CE：CTTTTATGTTCACAGTGCAATTGTTTTTTTTCTCTTGGAAAGAAAGT；NKV66005-CE：CATGCATATGCTATATAATGAAATCGTTTTTCTCTTGGAAAGAAAGT；NKV66006-CE：GCATTGTAGGTCAATTTCCGTTTTTTTTCTCTTGGAAAGAAAGT；NKV66007-LE：TGCAGGCTTCGTGGACGTTTTTTTGAAGTTACCGTTTT；NKV66008-LE：TTTGTAATACCTCGCTCAGATGGTTTTTCTGAGTCAAAGCAT；NKV66009-LE：ATCAGATAACTGTTTTTTAGTTATACTTTCTATTTTTGAAGTTACCGTTTT；NKV66010-LE：TGACATCGGTAGCACCTTATTGATAATTTTTCTGAGTCAAAGCAT；NKV66011-LE：CTGCAAACATGACCCGGTCTTTTTGAAGTTACCGTTTT；NKV66012-LE：CTTGTCTACTCGTATGTCTCCAACATTTTTCTGAGTCAAAGCAT；NKV66013-LE：CTTGCAACGTTGGTACTTTACCATTTTTGAAGTTACCGTTTT；NKV66014-LE：GCGGTGCAAGTTCTAATATAACCTTTTTTCTGAGTCAAAGCAT；NKV66015-LE：CTGAGCTGTCCAATTGCTCATTTTTTTGAAGTTACCGTTTT；NKV66016-LE：TCTATTTCATCCTCATCCTCATCCTTTTTTCTGAGTCAAAGCAT；NKV66017-LE：GGCCGCTCCAGCAAATGGTTTTTGAAGTTACCGTTTT；NKV66018-LE：TGTTCAGCTTGTCTAGCTTGCTGTTTTTTCTGAGTCAAAGCAT；NKV66019-BL：CACATGATAATCTAAGATCAAGTAAAGGTA；NKV66020-BL：AACTTGTAAGTTCCTTTTTGCAGTATA；NKV66021-BL：TCAATACATGCAAACCTATATAGCTCTA；NKV66022-BL：CAATAAACATACCCTACATACTGCATAT；NKV66023-BL：TATAGTACCTATATTTTCTAACCTTACTATAAAA；NKV66024-BL：TTCCTCCGGTGTTAACGGACAT；NKV66025-BL：TGCATGGTTATACTGTAGATTCTGTAG；

在其他通用探针、发光底物、信号放大前体、信号放大体以及AP报告探针均一致的前提下，以不同浓度的各***阳性细胞为试验对象进行化学发光检测，结果显示，同一浓度的阳性细胞标本，采用本发明探针序列所检测到的信号值明显高于对照探针序列，这表明了本发明所述探针序列对于检测***细胞有很高的特异性，能够改善检测的定量限(LOQ)、检测限(LOD)以及灵敏度。

由以上技术方案可知，本发明针对多种高危型人***瘤病毒型E6E7区域进行捕获延伸探针、K型标记探针以及封闭探针的设计和人为优化，进一步提高了基于类似bDNA和QuantiMAT技术对***细胞检测的特异性，表现出较佳的定量限、检测限以及灵敏度，并可应用于此类试剂盒的制备中。

附图说明

图1所示为本发明所述试剂盒工作原理示意图(载体为磁珠)；其中，1表示通用探针，2表示捕获延伸探针，3表示目标RNA，4表示K型标记探针，5表示信号放大前体，6表示信号放大体，7表示AP报告探针，8表示封闭探针；

图2所示为本发明所述试剂盒工作原理示意图(载体为细胞培养板)；其中，1表示通用探针，2表示捕获延伸探针，3表示目标RNA，4表示K型标记探针，5表示信号放大前体，6表示信号放大体，7表示AP报告探针，8表示封闭探针，9表示细胞培养板。

具体实施方式

本发明公开了一种***检测探针序列及其应用，本领域技术人员可以借鉴本文内容，适当改进工艺参数实现。特别需要指出的是，所有类似的替换和改动对本领域技术人员来说是显而易见的，它们都被视为包括在本发明。本发明所述探针序列及其应用已经通过实施例进行了描述，相关人员明显能在不脱离本发明内容、精神和范围内对本文所述探针序列及其应用进行改动或适当变更与组合，来实现和应用本发明技术。

依照本发明所提供的序列探针及其他配套试剂盒组分，其在具体检测时的工作步骤和原理如下：

裂解待测样本释放目标RNA；

然后加入本发明所述探针序列，捕获延伸探针的一部分与偶联载体(磁珠或类似96孔板的细胞培养板)的通用探针结合，另一部分与目标RNA结合；K型标记延伸探针与目标RNA结合；封闭探针用来封闭一些位点提高特异性；

探针组杂交后，逐步加入信号放大前体、放大体、碱性磷酸酶标记的探针和化学发光底物。根据上述原理，一组K型标记延伸探针可将信号放大400倍，一般一个目标RNA会结合20组K型标记延伸探针，所以一个RNA目标会被放大8000倍。最后用化学发光分析仪检测，原理示意图见图1和图2。

定量检测可以得到实际的倍数差异，而不是常规方法TaqMan中CT的变化结果，结果更直接准确，灵敏度比TaqMan高100～1000倍。

如未特别说明，本发明对比试验中除应有的技术特征区别外，其余试验环境和条件保持一致。

以下就本发明所提供的一种***检测探针序列及其应用做进一步说明。

实施例1：本发明所述检测***的试剂盒

1、探针序列

选自本发明所述高危型HPV16、18、31、33、35、39、45、51、52、56、58、59、66和68型中一种或两种以上的捕获延伸探针、K型标记延伸探针和封闭探针；

2、试剂盒配套组分

与磁珠或细胞培养板等载体偶联的通用探针：SEQIDNo.413所示；

信号放大前体：ATGCTTTGACTCAG/AAAACGGTAACTTC(放大前体前置引导区序列，两种)+AGGATCGTAAGTAACTAAGGACTACC(放大体前置引导区互补序列)+19次放大体前置引导区互补序列(SEQIDNo.414-415所示)；

信号放大体：GGTAGTCCTTAGTTACTTACGATCCT(放大体前置引导区序列)+GAATCCATTGAATCCTGTGATT(报告探针重复区互补序列)+19次报告探针重复区互补序列(SEQIDNo.416所示)；

AP标记的探针：AATCACAGGATTCAATGGATTC-AP(SEQIDNo.417所示)；

金刚烷衍生物。

实施例2：特异性对比试验

1、试验方法

采用HPV16\18型***阳性细胞(Caski和hela细胞)，用科蒂亚公司生产的细胞标本保存液配制成浓度为80个/mL、160个/mL、320个/mL、640个/mL和1280个/mL的四种细胞标本液；

标本裂解：

细胞标本液离心后沉淀细胞团，加入如下混合液：200μl裂解液(裂解液需提前放置37℃恒温箱20分钟)和400μl试验用水(混合比例为1：2)。用移液枪吹打细胞团，使细胞分散悬浮，再加入5μl蛋白酶K。65℃中放置1小时，裂解过程中进行2～3次震荡。1小时后取出，取上清液用于检测。

因目前市售的***阳性细胞只有HPV16\18型，其他***亚型(31\33\35\39\45\51\52\56\58\59\66\68)采用各自病毒的质控armored RNA，RNA浓度设置为0.12ng/ml、0.25ng/ml、0.5ng/ml、1ng/ml和2ng/ml；

同时，采用HPV阴性的细胞C33-A以及***低危型6b/11/42/43/44/53/72病毒的质控armored RNA进行特异性对比；

检测缓冲液配制：

取出检测探针、封闭反应液放在冰上融解，融解后需混匀离心。

标本检测缓冲液50ul/孔。配置方法如下：1ul封闭反应液+17ul裂解液+31ul试验用水+1ul检测探针。

探针配制：各组分浓度要求，探针组中CE探针：LE探针：BL探针的大致比例是1:4:2。各组分每条探针终浓度分别是CE要求1fmol/ul、LE要求4fmol/ul、BL要求2fmol/ul。

布板：

将包被有通用探针的96孔捕获包被板从4度冰箱中取出，在常温下复温。将上述缓冲液及待测上清液分别加入到96孔捕获包被板中：

空白对照孔：98ul空白对照缓冲液+2ul试验用水。

试验孔：98ul空白对照缓冲液+2ul待测上清。

保温条件和时间：用封板贴纸封紧整块板，放入55度温箱保温3.5小时。

信号放大：

将稀释液、10×洗脱液置37℃预热20分钟；信号放大前体，信号放大体取出放在冰上融解，震荡混匀，轻微离心。

配置所需液体：1×洗脱液：取10×洗脱液，用DDH₂O稀释为1×洗脱液,其体积比为10×洗脱液:DDH₂O＝1:9。

信号放大前体：溶液体积配比为信号放大前体：稀释液＝1:1000，取试剂盒所配信号放大前体加入到适量稀释液中；

信号放大体：溶液体积配比为信号放大体：稀释液＝1:1000，取试剂盒所配信号放大体加入到适量稀释液中；

AP标记探针：溶液体积配比为AP标记探针：稀释液＝1:1000，取试剂盒所配探针标记物加入到适量稀释液中；

(1)将96孔捕获包被板从温箱中取出，掀开封板贴纸，倒净板中液体，用1×洗脱液200ul/孔洗板洗3次。将所配信号放大前体每孔加入100ul，放入55度温箱保温40分钟。

(2)将96孔捕获包被板从温箱中取出，掀开封板贴纸，倒净板中液体，用1×洗脱液200ul/孔洗板洗3次。将所配信号放大体每孔加入100ul，放入55度温箱保温40分钟。

(3)将96孔捕获包被板从温箱中取出，掀开封板贴纸，倒净板中液体，用1×洗脱液200ul/孔洗板洗3次。将所配AP标记探针每孔加入100ul，放入50度温箱保温40分钟。

读板：

从4度冰箱取出底物金刚烷衍生物复温。复温后加入底物催化剂，底物与底物催化剂配比为1000:3，震荡混匀，使用前再次移液器吹打混匀。

将96孔捕获包被板从温箱中取出，掀开封板贴纸，倒净板中液体，用1×洗脱液200ul/孔洗板洗3次。将所配底物每孔加入100ul，放入46度温箱保温20分钟后取出，上冷光仪检测。

2、试验对象

本发明：实施例1序列探针及其试剂盒配套组分；

对照组：本发明提供的对照组探针及实施例1中试剂盒配套组分；

3、结果

表1

HPV16型阳性细胞数目(个/ml)	本发明检测平均值	对照组检测平均值
			80	1.98	1.2
160	4.74	3.36
			320	10.17	8.33
640	20.78	17.62
			1280	47.52	35.33

表2

HPV18型阳性细胞数目(个/ml)	本发明检测平均值	对照组检测平均值
			80	2.56	1.89
160	5.87	4.86
			320	11.78	10.21
640	24.32	21.53
			1280	60.78	56.33

表3

根据表1-表3数据可以明显看出，采用本发明探针序列能够获得更高的检测信号值，这表明本发明所述探针序列具备更高的检测特异性。同时，在线性关系上，本发明的R值均大于0.99，具备良好的线性关系，检测时结果更加准确。

此外，本发明通过对127例正常人样本的检测分析，采用百分位数法计算正常人群mRNA浓度的90％正常值上限为1.0；为验证该正常参考值的有效性，本发明检测了414例***等疾病患者样本，其阳性率均高于文献报道的数据；以1.0作为正常值上限在理论上和临床实践上均可行。因此，本发明确定人***瘤状病毒检测信号的正常参考值为0～1.0，当检测结果≥1.0时可判定为HPV感染存在，癌基因处于转录活动期。

基于上述判断标准，本发明采用***阴性细胞以及各低危型病毒armored RNA为质控品，分别用本发明提供的各探针序列进行检测，信号值均小于0.8，结果判定为阴性，这表明本发明探针序列特异性高，不会出现假阳性。

以上所述仅是本发明的优选实施方式，应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以做出若干改进和润饰，这些改进和润饰也应视为本发明的保护范围。

序列表

<110> 郑州科蒂亚生物技术有限公司

<120> 一种***检测探针序列及其应用

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<400> 15

attacccgac agctcagatg aggtttttga agttaccgtt tt 42

<210> 16

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 16

aggatgttat agacagtcca gctgtttttc tgagtcaaag cat 43

<210> 17

<211> 51

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 17

aattacaata tcgttacctt ttgttgtcag tgtttttgaa gttaccgttt t 51

<210> 18

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 18

tgagtctaca cttcgtttgt gtgtactttt tctgagtcaa agcat 45

<210> 19

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 19

agagcacaca agtagatatt cgcatatttt tttgaagtta ccgtttt 47

<210> 20

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 20

gcaagagctg ttaatgggct catttttttc tgagtcaaag cat 43

<210> 21

<211> 28

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 21

atgttcaaaa atcctgcaga aagacctc 28

<210> 22

<211> 28

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 22

cattggaaat accctacgat gaactaag 28

<210> 23

<211> 33

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 23

tatagatata gtgtgtatgg aacaacatta gaa 33

<210> 24

<211> 29

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 24

gataaaaaga aacgattcca caacatagg 29

<210> 25

<211> 21

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 25

aggaaggtgg acaggacgtt g 21

<210> 26

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 26

catagtatgt tggagaagac ctcg 24

<210> 27

<211> 21

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 27

tggaatcgtg tgccccaact g 21

<210> 28

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 28

atgtttcaag acatgtttca agacactgtt tttctcttgg aaagaaagt 49

<210> 29

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 29

gtgccaagca ttggagacaa ctattttttc tcttggaaag aaagt 45

<210> 30

<211> 54

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 30

aggtatatga ttttgcattt gcagatttaa cagtttttct cttggaaaga aagt 54

<210> 31

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 31

ttgtatatag agagggaaat ccatttggtt tttctcttgg aaagaaagt 49

<210> 32

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 32

tatactgcta tgagcaatta agtgacagtt tttctcttgg aaagaaagt 49

<210> 33

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 33

tacagcaagt gacctacgaa ccattttttc tcttggaaag aaagt 45

<210> 34

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 34

acacaacatt gaactacagt gcgtgttttt gaagttaccg tttt 44

<210> 35

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 35

gaatgcaaaa aacctttgca acgatctgtt tttctgagtc aaagcat 47

<210> 36

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 36

aatatgtaaa ctgtgtttgc ggttcttatc tttttgaagt taccgtttt 49

<210> 37

<211> 60

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 37

taaaattagt gaatatagac attataatta ttctgtatat gtttttctga gtcaaagcat 60

<210> 38

<211> 56

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 38

gaaatacatt agaacaaaca gttaaaaaac ctttaaattt ttgaagttac cgtttt 56

<210> 39

<211> 55

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 39

tgaaatatta attaggtgta ttatatgtca aagacctttt tctgagtcaa agcat 55

<210> 40

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 40

ccgacgtaga gaaactgcac tgtttttgaa gttaccgttt t 41

<210> 41

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 41

tgacgtgtaa aaacgccatg agagtttttc tgagtcaaag cat 43

<210> 42

<211> 46

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 42

gacacaagcc aacgttaaag gaatatgttt ttgaagttac cgtttt 46

<210> 43

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 43

ttttagattt atatcctgaa ccaactgacc tttttctgag tcaaagcat 49

<210> 44

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 44

accggccaga tggacaagca tttttgaagt taccgtttt 39

<210> 45

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 45

caaccagcca cagctgatta ctactttttc tgagtcaaag cat 43

<210> 46

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 46

attgtaacct gttgtcacac ttgtaacatt tttgaagtta ccgtttt 47

<210> 47

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 47

ccacagttcg tttatgtgtc aacagttttt ctgagtcaaa gcat 44

<210> 48

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 48

acagcaacta cttatgggca cagttttttg aagttaccgt ttt 43

<210> 49

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 49

gaatattgtg tgccctacct gtgctttttc tgagtcaaag cat 43

<210> 50

<211> 28

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 50

aggaaaaacc acgaacattg catgattt 28

<210> 51

<211> 29

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 51

tttgtgtcct caagaaaaaa aacgacatg 29

<210> 52

<211> 33

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 52

tggatttaaa caaacgattt cataatattt cgg 33

<210> 53

<211> 17

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 53

gtcgttgggc agggcgc 17

<210> 54

<211> 20

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 54

tgtgcggcgt gttggaggtc 20

<210> 55

<211> 23

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 55

ctcagatgag gatgaaggct tgg 23

<210> 56

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 56

gaggtagaag aaagcatcca tgaaattttt tttctcttgg aaagaaagt 49

<210> 57

<211> 54

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 57

gtttgaattg tgtatactgc aaacaagaat tactttttct cttggaaaga aagt 54

<210> 58

<211> 46

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 58

ttttagattt ggaacccgag gcaacttttt ctcttggaaa gaaagt 46

<210> 59

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 59

agatactatt gacggtccag ctggtttttc tcttggaaag aaagt 45

<210> 60

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 60

acaagcaaaa ccagacacct ccaatttttc tcttggaaag aaagt 45

<210> 61

<211> 46

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 61

tacagagcac acacattgac atacgttttt ctcttggaaa gaaagt 46

<210> 62

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 62

aatagtgtgc cccggctgtt ctttttctct tggaaagaaa gt 42

<210> 63

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 63

atgtttcagg acccagctga acgtttttga agttaccgtt tt 42

<210> 64

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 64

accttacaaa ctgcatgatt tgtgcaactt tttctgagtc aaagcat 47

<210> 65

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 65

agcggagtga ggtatatgac tttgtttttg aagttaccgt ttt 43

<210> 66

<211> 52

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 66

catgctatga tttgtgtata gtatatagag aagtttttct gagtcaaagc at 52

<210> 67

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 67

gccagccata tggagtatgc atgtttttga agttaccgtt tt 42

<210> 68

<211> 61

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 68

aaatgtttaa aattttattc aaaaataagt gaatatagat ggtttttctg agtcaaagca 60

t 61

<210> 69

<211> 50

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 69

tatagatata gtgtgtatgg agaaacgtta gtttttgaag ttaccgtttt 50

<210> 70

<211> 56

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 70

aaaaacaatg caacaaacag ttatgtcatt tattaatttt ttctgagtca aagcat 56

<210> 71

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 71

taggtgtatt acatgtcaaa aaccgctgtt tttgaagtta ccgtttt 47

<210> 72

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 72

tgtccagttg aaaagcaaag acatttagtt tttctgagtc aaagcat 47

<210> 73

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 73

tagagaaacc gaggtgtaat catgcttttt gaagttaccg tttt 44

<210> 74

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 74

atggagaaat aactacattg caagactatg tttttctgag tcaaagcat 49

<210> 75

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 75

tgacctatac tgttatgagc aattgtgttt tttgaagtta ccgtttt 47

<210> 76

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 76

gacagctcag aggaggagga tttttctgag tcaaagcat 39

<210> 77

<211> 52

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 77

ttataatatt gtaacgtcct gttgtaaatg tgatttttga agttaccgtt tt 52

<210> 78

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 78

ggcgacacta cgtctgtgtg tttttctgag tcaaagcat 39

<210> 79

<211> 30

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 79

aagaaaaaaa acgattccat aacatcggtg 30

<210> 80

<211> 19

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 80

gacggtggac aggtcggtg 19

<210> 81

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 81

tatgtcctgt tggaaaccaa cacg 24

<210> 82

<211> 33

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 82

taaattggaa gatttattaa tgggcacatt tgg 33

<210> 83

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 83

atggcgcgat ttcacaatcc tgctttttct cttggaaaga aagt 44

<210> 84

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 84

ccaccttgca ggacattaca atagtttttc tcttggaaag aaagt 45

<210> 85

<211> 55

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 85

ccaatcatgt ataaaatttt atgctaaaat acggtttttc tcttggaaag aaagt 55

<210> 86

<211> 54

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 86

agaaaaatta agacacctaa atagcaaacg aagtttttct cttggaaaga aagt 54

<210> 87

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 87

ccacaaaacg ggaggaccgc tttttctctt ggaaagaaag t 41

<210> 88

<211> 46

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 88

cccaccttgc aggaaattgt attagttttt ctcttggaaa gaaagt 46

<210> 89

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 89

aacatcaact actagccaga cgggtttttc tcttggaaag aaagt 45

<210> 90

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 90

actgcagctg gtagtagaag cctttttctc ttggaaagaa agt 43

<210> 91

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 91

agaacggcca tacaaattgc cagatttttg aagttaccgt ttt 43

<210> 92

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 92

cctgtgcaca acgctggaca tttttctgag tcaaagcat 39

<210> 93

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 93

cctgtgtcta ttgcagacga cctttttgaa gttaccgttt t 41

<210> 94

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 94

actacagcaa accgaggtat atgaattttt tttctgagtc aaagcat 47

<210> 95

<211> 52

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 95

gcatttagtg atttatatgt agtatatagg gactttttga agttaccgtt tt 52

<210> 96

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 96

ggggaaccac tagctgcatg tttttctgag tcaaagcat 39

<210> 97

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 97

gagctacgat attactcgga ctcgtttttg aagttaccgt ttt 43

<210> 98

<211> 54

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 98

gtgtatgcaa ctacattaga aaatataact aatacttttt ctgagtcaaa gcat 54

<210> 99

<211> 56

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 99

aaagttatat aatttattaa taaggtgcat gtgttgtttt ttgaagttac cgtttt 56

<210> 100

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 100

ctgaaaccgc tgtgtccagc tttttctgag tcaaagcat 39

<210> 101

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 101

atttcataaa atagcaggaa gctatacagg tttttgaagt taccgtttt 49

<210> 102

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 102

acagtgtcga cggtgctgga tttttctgag tcaaagcat 39

<210> 103

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 103

agactaacac gaagagaaac ccaagttttt gaagttaccg tttt 44

<210> 104

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 104

tataacatca gatatgcgtg gaccaaagtt tttctgagtc aaagcat 47

<210> 105

<211> 48

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 105

atttatgtcc ttacaatgaa atacagccgt ttttgaagtt accgtttt 48

<210> 106

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 106

gttgaccttg tatgtcacga gcaatttttc tgagtcaaag cat 43

<210> 107

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 107

ttaggagagt cagaggatga aatagatgtt tttgaagtta ccgtttt 47

<210> 108

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 108

aacccgacca tgcagttaat cacctttttc tgagtcaaag cat 43

<210> 109

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 109

atgaaccaca gcgtcacaca atactttttg aagttaccgt ttt 43

<210> 110

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 110

agtgttcgtg ttgtaagtgt aacaacactt tttctgagtc aaagcat 47

<210> 111

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 111

tcacgggata ctctgcgaca actttttgaa gttaccgttt t 41

<210> 112

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 112

tacagcagct gtttatggac tcactttttc tgagtcaaag cat 43

<210> 113

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 113

taggatttgt gtgtccgtgg tgtg 24

<210> 114

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 114

atggcgcgct ttgacgatcc tttttctctt ggaaagaaag t 41

<210> 115

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 115

aacgcaacga ccctacaagc tactttttct cttggaaaga aagt 44

<210> 116

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 116

tatattgcaa agcaacattg gaacgcactt tttctcttgg aaagaaagt 49

<210> 117

<211> 52

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 117

agagacactg gaaaaaataa ctaatacaga gtttttctct tggaaagaaa gt 52

<210> 118

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 118

gcaagaaaga cttcgcagac gtagtttttc tcttggaaag aaagt 45

<210> 119

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 119

ggaaacacaa gtatagcaat aagtatgctt tttctcttgg aaagaaagt 49

<210> 120

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 120

agaggaggaa aacgatgaag cagatttttc tcttggaaag aaagt 45

<210> 121

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 121

cttacagtag agagctcggc agtttttctc ttggaaagaa agt 43

<210> 122

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 122

cagatttgtg cacagaattg aatacatctt tttgaagtta ccgtttt 47

<210> 123

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 123

actacaagac gtatctattg cctgtgtttt tctgagtcaa agcat 45

<210> 124

<211> 57

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 124

agaggtatat caatttgctt ttaaagattt atttatagtt tttgaagtta ccgtttt 57

<210> 125

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 125

tgtatagaga ctgtatagca tatgctgctt tttctgagtc aaagcat 47

<210> 126

<211> 52

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 126

atgccataaa tgtatagact tttattccag aattttttga agttaccgtt tt 52

<210> 127

<211> 55

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 127

tagagaatta agatattatt caaactctgt atatggtttt tctgagtcaa agcat 55

<210> 128

<211> 48

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 128

ttgtataatt tgttaataag gtgcctgcgt ttttgaagtt accgtttt 48

<210> 129

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 129

gtgccagaaa ccattgaacc cagtttttct gagtcaaagc at 42

<210> 130

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 130

cagaaaaacg tagacacctt aaggactttt tgaagttacc gtttt 45

<210> 131

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 131

aaacgaagat tccacagcat agctgttttt ctgagtcaaa gcat 44

<210> 132

<211> 38

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 132

ggcagtaccg agggcagtgt ttttgaagtt accgtttt 38

<210> 133

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 133

taatacatgt tgtgaccagg cacgtttttc tgagtcaaag cat 43

<210> 134

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 134

agaatgaatt agatcctgtt gacctgtttt tttgaagtta ccgtttt 47

<210> 135

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 135

gtgttacgag caattaagcg agtctttttc tgagtcaaag cat 43

<210> 136

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 136

acagcgtcac aaaattttgt gtgtatgttt tttgaagtta ccgtttt 47

<210> 137

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 137

tgtaagtgtg acggcagaat tgagtttttc tgagtcaaag cat 43

<210> 138

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 138

atgaccttag aacactacag cagctttttg aagttaccgt ttt 43

<210> 139

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 139

tgtttttgag caccttgtcc tttgtgtttt tctgagtcaa agcat 45

<210> 140

<211> 20

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 140

atggacccca ggcaacactg 20

<210> 141

<211> 28

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 141

caagaaattg tattgcattt ggaacctc 28

<210> 142

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 142

tggcgttagt catgcacaac tacc 24

<210> 143

<211> 18

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 143

agcccgacga gccgaacc 18

<210> 144

<211> 28

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 144

acagcgtcac aaaattttgt gtgtatgt 28

<210> 145

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 145

tgtaagtgtg acggcagaat tgag 24

<210> 146

<211> 22

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 146

tgtccgtggt gtgcaactaa cc 22

<210> 147

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 147

atgttcgaag acaatgttcg aagacaagtt tttctcttgg aaagaaagt 49

<210> 148

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 148

agggaaagac cacgaacgct gtttttctct tggaaagaaa gt 42

<210> 149

<211> 50

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 149

ggataataat ccatatgcag tatgcaaact ttttctcttg gaaagaaagt 50

<210> 150

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 150

aaagaccact tgggcctgaa gaaatttttc tcttggaaag aaagt 45

<210> 151

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 151

acggggcaat gcgctaattg ctttttctct tggaaagaaa gt 42

<210> 152

<211> 46

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 152

tagataatat gcgtgaccag ctaccttttt ctcttggaaa gaaagt 46

<210> 153

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 153

agaaagacgg gctggacagg tttttctctt ggaaagaaag t 41

<210> 154

<211> 48

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 154

catgaattat gtgaagcttt gaacgtttct ttttgaagtt accgtttt 48

<210> 155

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 155

tatgcacaat atacaggtag tgtgtgtgtt tttctgagtc aaagcat 47

<210> 156

<211> 52

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 156

tattgtaaaa aggaattatg tagagcagat gtatttttga agttaccgtt tt 52

<210> 157

<211> 57

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 157

tataatgtag catttactga aattaagatt gtatatagtt tttctgagtc aaagcat 57

<210> 158

<211> 56

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 158

aatgtttact gttttattca aaaattagag agtatagttt ttgaagttac cgtttt 56

<210> 159

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 159

acgttatagc aggtctgtgt atggtttttc tgagtcaaag cat 43

<210> 160

<211> 52

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 160

tactacatta gaggcaatta ctaaaaaaag ctttttttga agttaccgtt tt 52

<210> 161

<211> 51

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 161

atatgattta tcgataaggt gtcatagatg tctttttctg agtcaaagca t 51

<210> 162

<211> 48

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 162

agcaaaaatt ggtggacgaa aaaaaaaggt ttttgaagtt accgtttt 48

<210> 163

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 163

ttccatgaaa tagcgggacg ttggtttttc tgagtcaaag cat 43

<210> 164

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 164

tggcaacgta cacgacaacg taactttttg aagttaccgt ttt 43

<210> 165

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 165

gaaacccaag tgtaataaag ccatgctttt tctgagtcaa agcat 45

<210> 166

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 166

gtggtaatgt accacaatta aaagatgtag tttttgaagt taccgtttt 49

<210> 167

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 167

tattgcattt aacaccacag actgaaattg tttttctgag tcaaagcat 49

<210> 168

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 168

acttgcaatg ctacgagcaa tttgactttt tgaagttacc gtttt 45

<210> 169

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 169

agctcagagg aggaggatga agtttttctg agtcaaagca t 41

<210> 170

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 170

ctacgtgtta cagaattgaa gctccttttt gaagttaccg tttt 44

<210> 171

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 171

gtgttgcagg tgttcaagtg tagttttttc tgagtcaaag cat 43

<210> 172

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 172

cagatgttaa tgggcgaact gagctttttg aagttaccgt ttt 43

<210> 173

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 173

ctggtttgcc cgtgttgtgc tttttctgag tcaaagcat 39

<210> 174

<211> 23

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 174

acaactggca gtggaaagca gtg 23

<210> 175

<211> 23

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 175

gagacactct tcgcgttgta cag 23

<210> 176

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 176

ggtgcatgaa ataaggctgc agtgtttttc tcttggaaag aaagt 45

<210> 177

<211> 57

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 177

atttacgaat agtatataga gacaataatc catatgtttt tctcttggaa agaaagt 57

<210> 178

<211> 54

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 178

ggtaagaaaa ccattaagtg aaataactat tagtttttct cttggaaaga aagt 54

<210> 179

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 179

tgtgcagtgc aaaaaagagc tacaactttt tgaagttacc gtttt 45

<210> 180

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 180

gaagagaggt atacaagttt ctatttacag tttttctgag tcaaagcat 49

<210> 181

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 181

gcgtgtgtat tatgtgccta cgctttttga agttaccgtt tt 42

<210> 182

<211> 56

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 182

tttttatcta agataagtga atataggcat tatcaatttt ttctgagtca aagcat 56

<210> 183

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 183

gcgatttcat aatattatgg gtcgttggtt tttgaagtta ccgtttt 47

<210> 184

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 184

acagggcgct gttcagagtg tttttctgag tcaaagcat 39

<210> 185

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 185

cccaagtgta acgtcatgcg tgtttttgaa gttaccgttt t 41

<210> 186

<211> 55

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 186

gagacaaagc aactataaaa gattatatat tagatctttt tctgagtcaa agcat 55

<210> 187

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 187

tgcaacctga aacaactgac ctactttttg aagttaccgt ttt 43

<210> 188

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 188

actgctatga gcaattaggt gacagttttt ctgagtcaaa gcat 44

<210> 189

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 189

ctcagatgag gaggatacag atggtttttg aagttaccgt ttt 43

<210> 190

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 190

tgtggaccgg ccagatggac tttttctgag tcaaagcat 39

<210> 191

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 191

aagcagaaca agccacaagc aattactttt tgaagttacc gtttt 45

<210> 192

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 192

tacattgtga catattgtca cagttgtgtt tttctgagtc aaagcat 47

<210> 193

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 193

cactgcgacg gaccttcgta tttttgaagt taccgtttt 39

<210> 194

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 194

ctctacagca gatgctgttg ggtttttctg agtcaaagca t 41

<210> 195

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 195

atgtttgagg atccagcaac acga 24

<210> 196

<211> 16

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 196

ccccggaccc tgcacg 16

<210> 197

<211> 25

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 197

aattgtgtga ggtgctggaa gaatc 25

<210> 198

<211> 31

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 198

attcacttta tgggaaaaca ttagaagaga g 31

<210> 199

<211> 30

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 199

atgtataatt tgtcaaacgc cattatgtcc 30

<210> 200

<211> 33

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 200

tgaagaaaaa gaaagacatg ttaatgcaaa caa 33

<210> 201

<211> 20

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 201

ttggagaccc cgacctgtga 20

<210> 202

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 202

ctcagatgag gaggatacag atgg 24

<210> 203

<211> 20

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 203

tgtggaccgg ccagatggac 20

<210> 204

<211> 23

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 204

cacattacaa gttgtgtgcc ccg 23

<210> 205

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 205

aaaaaagaac taacacgtgc tgaggtattt tttctcttgg aaagaaagt 49

<210> 206

<211> 51

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 206

aacgctgcaa gaagttatat tagaactaac tttttctctt ggaaagaaag t 51

<210> 207

<211> 48

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 207

acctcaaaca gaaattgacc tacagtgttt ttctcttgga aagaaagt 48

<210> 208

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 208

agcaagctag acaagctaaa caacatactt tttctcttgg aaagaaagt 49

<210> 209

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 209

atggagccac aattcaacaa tccacttttt gaagttaccg tttt 44

<210> 210

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 210

aggaacgtcc acgaagcctg tttttctgag tcaaagcat 39

<210> 211

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 211

caccacttga gtgaggtatt agaaatactt tttgaagtta ccgtttt 47

<210> 212

<211> 54

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 212

ctttaattga tcttagatta tcatgtgtat attgcttttt ctgagtcaaa gcat 54

<210> 213

<211> 56

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 213

ataattttgc atgcactgaa ttaaaattag tgtatagttt ttgaagttac cgtttt 56

<210> 214

<211> 46

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 214

ggataatttt ccttatgcag tgtgcagttt ttctgagtca aagcat 46

<210> 215

<211> 59

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 215

agtatgttta ttgttttata gtaaagttag aaaatatagg tttttgaagt taccgtttt 59

<210> 216

<211> 48

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 216

tattatgact attcagtgta tggagctact ttttctgagt caaagcat 48

<210> 217

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 217

tgcattgtga cagaaaaaga cgatttcatt tttgaagtta ccgtttt 47

<210> 218

<211> 40

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 218

gtcatgtttg gggtgctgga gtttttctga gtcaaagcat 40

<210> 219

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 219

acaaacatct agagaaccta gagaatcttt tttgaagtta ccgtttt 47

<210> 220

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 220

acagtataat catgcatggt aaagtacctt tttctgagtc aaagcat 47

<210> 221

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 221

ggatgaggat gaggatgaag tagatttttg aagttaccgt ttt 43

<210> 222

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 222

ccatttgcag gagcggccac tttttctgag tcaaagcat 39

<210> 223

<211> 46

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 223

gtgttaccta atacacgtac cttgttgttt ttgaagttac cgtttt 46

<210> 224

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 224

tcagtgtaag tttgtggtgc agttgttttt ctgagtcaaa gcat 44

<210> 225

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 225

gacattcaga gtaccaaaga ggactttttg aagttaccgt ttt 43

<210> 226

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 226

ctgcgtgttg tacaacagct gctttttctg agtcaaagca t 41

<210> 227

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 227

ttatgggtgc gttaacagta acgtgttttt gaagttaccg tttt 44

<210> 228

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 228

cccactctgc gcatcaagta actttttctg agtcaaagca t 41

<210> 229

<211> 37

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 229

actagaaagt ataactaaaa aacagttatg tgattta 37

<210> 230

<211> 28

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 230

ttaataaggt gctacagatg tcaaagtc 28

<210> 231

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 231

cgttaactcc ggaggaaaag caat 24

<210> 232

<211> 28

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 232

tgcattgtga cagaaaaaga cgatttca 28

<210> 233

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 233

tctaatagca catggttgga ccgg 24

<210> 234

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 234

caatgagcaa ttggacagct caga 24

<210> 235

<211> 55

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 235

gtatatgact ttacatttgc agatttaaga atagtttttc tcttggaaag aaagt 55

<210> 236

<211> 51

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 236

gagacacatt agaacaaaca ctaaaaaagc tttttctctt ggaaagaaag t 51

<210> 237

<211> 51

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 237

aagaccattg tgtccacaag aaaaaaaaag tttttctctt ggaaagaaag t 51

<210> 238

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 238

agcacaaccg gccacagcta tttttctctt ggaaagaaag t 41

<210> 239

<211> 40

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 239

atgttccagg acgcagagga gtttttgaag ttaccgtttt 40

<210> 240

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 240

aaaccacgga cattgcatga tttgtgtttt tctgagtcaa agcat 45

<210> 241

<211> 48

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 241

catgaaatcg aattgaaatg cgttgaatgt ttttgaagtt accgtttt 48

<210> 242

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 242

caaaaagact ttgcagcgat ctgagttttt ctgagtcaaa gcat 44

<210> 243

<211> 52

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 243

atgtaaagtg tgtttacgat tgctatctaa aattttttga agttaccgtt tt 52

<210> 244

<211> 54

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 244

aagtgagtat agacattata attattcgct atatgttttt ctgagtcaaa gcat 54

<210> 245

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 245

cccgacgtag acaaacacaa gtgtttttga agttaccgtt tt 42

<210> 246

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 246

taacctgtaa caacgccatg agagtttttc tgagtcaaag cat 43

<210> 247

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 247

gaaacaaccc aacgctaaga gaatatattt tttgaagtta ccgtttt 47

<210> 248

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 248

tttagattta catcctgaac caactgactt tttctgagtc aaagcat 47

<210> 249

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 249

ctattctgct atgagcaatt atgtgacatt tttgaagtta ccgtttt 47

<210> 250

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 250

gctcagacga ggatgaaata ggctttttct gagtcaaagc at 42

<210> 251

<211> 50

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 251

attactacat tgtaacgtgt tgttacactt gtttttgaag ttaccgtttt 50

<210> 252

<211> 40

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 252

tgacgccacg gttcgtttgt gtttttctga gtcaaagcat 40

<210> 253

<211> 46

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 253

tatcaacagt acagcaactg aagtacgttt ttgaagttac cgtttt 46

<210> 254

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 254

aaccctacag cagctgctta tggtttttct gagtcaaagc at 42

<210> 255

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 255

gcacatgtac tattgtgtgc cctatttttg aagttaccgt ttt 43

<210> 256

<211> 22

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 256

tcaggcgttg gagacatctg tg 22

<210> 257

<211> 28

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 257

tgtatagaga tggaaatcca tttgcagt 28

<210> 258

<211> 37

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 258

gtttagagga aatattaatt agatgtatta tttgtca 37

<210> 259

<211> 33

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 259

gcatgtggat ttaaacaaaa ggtttcataa tat 33

<210> 260

<211> 20

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 260

ttcgggtcgt tggacagggc 20

<210> 261

<211> 21

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 261

gctgtgcagt gtgttggaga c 21

<210> 262

<211> 20

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 262

ttggacgggc cagatggaca 20

<210> 263

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 263

caaaggggaa ctgcaagaaa gagatttttc tcttggaaag aaagt 45

<210> 264

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 264

taagacagca acgacaagcg cgtttttctc ttggaaagaa agt 43

<210> 265

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 265

gaagttgacc ttgtgtgcta cgagtttttc tcttggaaag aaagt 45

<210> 266

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 266

caattacctg actccgactc cgtttttctc ttggaaagaa agt 43

<210> 267

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 267

agctgaacca cagcgtcaca actttttctc ttggaaagaa agt 43

<210> 268

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 268

atggcacgct ttgaggatcc tactttttga agttaccgtt tt 42

<210> 269

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 269

acaacgacca tacaaactgc ctgatttttc tgagtcaaag cat 43

<210> 270

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 270

cttgagcaca acattgaata ttcctctgtt tttgaagtta ccgtttt 47

<210> 271

<211> 48

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 271

catgatattc gcatcaattg tgtgttttgt ttttctgagt caaagcat 48

<210> 272

<211> 56

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 272

ggtatttgaa tttgctttta atgacttatt tatagtgttt ttgaagttac cgtttt 56

<210> 273

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 273

tatagagact gtacaccgta tgcagttttt ctgagtcaaa gcat 44

<210> 274

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 274

cgtgtctgaa atgcatttca ttttatgctt tttgaagtta ccgtttt 47

<210> 275

<211> 54

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 275

aagagtaaga gaattaagat attatagaga ttctgttttt ctgagtcaaa gcat 54

<210> 276

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 276

tgtatggaga aacattagag gctgaaactt tttgaagtta ccgtttt 47

<210> 277

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 277

caagacaccg ttacatgagc tgctttttct gagtcaaagc at 42

<210> 278

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 278

tgatacgctg ttatagatgc ctaaaacctt tttgaagtta ccgtttt 47

<210> 279

<211> 52

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 279

tctatgtcca acagacaaat taaagcatat aactttttct gagtcaaagc at 52

<210> 280

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 280

atatacagga cagtgtcgtg ggtgtttttg aagttaccgt ttt 43

<210> 281

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 281

tcggacccga gcaagacacc tttttctgag tcaaagcat 39

<210> 282

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 282

tagtgaaaca ctggtgtaaa acaatgcatt tttgaagtta ccgtttt 47

<210> 283

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 283

tggaccaaaa gcaacacttt gtgacttttt ctgagtcaaa gcat 44

<210> 284

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 284

agaatgaaaa agatgaacca gatggagttt tttgaagtta ccgtttt 47

<210> 285

<211> 46

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 285

taatcatcct ttgctactag ctagacgttt ttctgagtca aagcat 46

<210> 286

<211> 50

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 286

attgtgtgtg tgtgttgtaa gtgtaataat ctttttgaag ttaccgtttt 50

<210> 287

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 287

aacttcagct agtagtagaa acctcgtttt tctgagtcaa agcat 45

<210> 288

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 288

caagacggat tgcgagcctt actttttgaa gttaccgttt t 41

<210> 289

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 289

agcagctgtt tatggacgca ctattttttc tgagtcaaag cat 43

<210> 290

<211> 33

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 290

tgaaaaaaga agattccata atatagctgg aat 33

<210> 291

<211> 33

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 291

attgttttag atttggaacc acacaatttt gag 33

<210> 292

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 292

cctttgtgtg tcctttgtgt gcag 24

<210> 293

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 293

catctgagcg aggtattaca aatacctttt tctcttggaa agaaagt 47

<210> 294

<211> 58

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 294

acattagaaa gtataactaa aaaacagtta tctgattttt ttctcttgga aagaaagt 58

<210> 295

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 295

aagtagacaa gctacagaat ctacagtatt tttctcttgg aaagaaagt 49

<210> 296

<211> 46

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 296

cgcaaacgga aattgaccta caatgttttt ctcttggaaa gaaagt 46

<210> 297

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 297

gcaagctaga caagctgaac aacatttttc tcttggaaag aaagt 45

<210> 298

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 298

tgcccactct gcgcatcatc tttttctctt ggaaagaaag t 41

<210> 299

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 299

atggattcca tattcagcaa tacacaggtt tttgaagtta ccgtttt 47

<210> 300

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 300

aacgtccacg aagcctgcac tttttctgag tcaaagcat 39

<210> 301

<211> 52

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 301

tttacttgat cttagattat catgtgtata ctgtttttga agttaccgtt tt 52

<210> 302

<211> 52

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 302

caaaaaggaa cttacaagtt tagagttata tagtttttct gagtcaaagc at 52

<210> 303

<211> 52

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 303

gtttgcatgt attgagttaa aactagtata tagtttttga agttaccgtt tt 52

<210> 304

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 304

aaacaattgg ccatatgcag tatgtaggtt tttctgagtc aaagcat 47

<210> 305

<211> 57

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 305

gtatgtttat tgttttatag taaggttaga aaatatagtt tttgaagtta ccgtttt 57

<210> 306

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 306

gtactataaa tattcagtgt atggggcatt tttctgagtc aaagcat 47

<210> 307

<211> 48

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 307

attgcactgt gaacataaaa gacgatttct ttttgaagtt accgtttt 48

<210> 308

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 308

attatatagc atatgcatgg accgggtttt tctgagtcaa agcat 45

<210> 309

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 309

taaccatgca tggtaaagta ccaacgtttt tgaagttacc gtttt 45

<210> 310

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 310

ttgcaagagg ttatattaga acttgcactt tttctgagtc aaagcat 47

<210> 311

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 311

ggatgaggat gaggatgaaa tagacttttt gaagttaccg tttt 44

<210> 312

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 312

catttgctgg agcggccaca tttttctgag tcaaagcat 39

<210> 313

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 313

taagtgttac ctaattcacg taccttgttt tttgaagtta ccgtttt 47

<210> 314

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 314

tgtaagtgtg agttggtggt gcagtttttc tgagtcaaag cat 43

<210> 315

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 315

agctacgtgt ggtacaacag ctgtttttga agttaccgtt tt 42

<210> 316

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 316

cttatgggtg cgttaacagt aacgtttttc tgagtcaaag cat 43

<210> 317

<211> 28

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 317

tatcaataag gtgctaccga tgtcaatg 28

<210> 318

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 318

tccgttaaca ccggaggaaa aaca 24

<210> 319

<211> 26

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 319

tcatgtttgc agtgttggag acatac 26

<210> 320

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 320

caatgagcaa ttggacagct caga 24

<210> 321

<211> 25

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 321

ttggacattc agagtaccaa agagg 25

<210> 322

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 322

ggacattgga caccacattg catgtttttc tcttggaaag aaagt 45

<210> 323

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 323

gaatcggtgt atgcaacaac attagaaatt tttctcttgg aaagaaagt 49

<210> 324

<211> 58

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 324

ccataactaa tacaaagtta tatgatttat caataagttt ttctcttgga aagaaagt 58

<210> 325

<211> 54

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 325

cctaaattca aaacgaagat ttcataaaat agctttttct cttggaaaga aagt 54

<210> 326

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 326

ggcaggaaac acaagtttaa ctaactattt tttctcttgg aaagaaagt 49

<210> 327

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 327

atcaactact agccagacgg gactttttct cttggaaaga aagt 44

<210> 328

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 328

gaacaacagc gtcacacaat tcagtttttc tcttggaaag aaagt 45

<210> 329

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 329

gggagaacct gcggaagcta tttttctctt ggaaagaaag t 41

<210> 330

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 330

tcacaaccct gaggaacggc tttttgaagt taccgtttt 39

<210> 331

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 331

catacaaatt gccagacctg tgcatttttc tgagtcaaag cat 43

<210> 332

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 332

acgttacaat agactgtgtc tattgcagtt tttgaagtta ccgtttt 47

<210> 333

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 333

aaggcaacta caacggacag aggtttttct gagtcaaagc at 42

<210> 334

<211> 52

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 334

tatatgaatt tgcctttggt gacttaaatg tagtttttga agttaccgtt tt 52

<210> 335

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 335

tatataggga cggggtacca ttagtttttc tgagtcaaag cat 43

<210> 336

<211> 50

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 336

ctgcatgcca atcatgtatt aaattttatg ctttttgaag ttaccgtttt 50

<210> 337

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 337

gaaaatacgg gaactacgat attactcatt tttctgagtc aaagcat 47

<210> 338

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 338

gtgcatgtgt tgcctgaaac catttttttg aagttaccgt ttt 43

<210> 339

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 339

gagtcctgct gaaaaactaa ggcatttttc tgagtcaaag cat 43

<210> 340

<211> 40

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 340

ccactgctgg accagtaaac gtttttgaag ttaccgtttt 40

<210> 341

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 341

agaggaccgc agacgcacac tttttctgag tcaaagcat 39

<210> 342

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 342

atgcaatgaa atagagccgg ttgacttttt gaagttaccg tttt 44

<210> 343

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 343

cttgtatgtc acgagcaatt aggagttttt ctgagtcaaa gcat 44

<210> 344

<211> 46

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 344

attcagacga tgaaatagat gaacccgttt ttgaagttac cgtttt 46

<210> 345

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 345

accatgcagt taatcaccac caactttttc tgagtcaaag cat 43

<210> 346

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 346

tgtacgtgtt gtaagtgtaa caacctactt tttgaagtta ccgtttt 47

<210> 347

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 347

tgcaactagt agtagaagcg tcgctttttc tgagtcaaag cat 43

<210> 348

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 348

gaactgctgt ttatggactc actaaatttt tttgaagtta ccgtttt 47

<210> 349

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 349

ttgtgtgtcc gtggtgtgca actttttctg agtcaaagca t 41

<210> 350

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 350

aggaaacttt acaggacagt gtcg 24

<210> 351

<211> 20

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 351

gcatggacca aagcccaccg 20

<210> 352

<211> 28

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 352

tgcaggaaat tgtgttagag ttatgtcc 28

<210> 353

<211> 54

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 353

cttacagagg tatttgaatt tgcatttaaa gattttttct cttggaaaga aagt 54

<210> 354

<211> 60

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 354

atttttattc tagaattaga gaattaagac attattcagt ttttctcttg gaaagaaagt 60

<210> 355

<211> 40

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 355

aaggtgcctg cggtgccagt ttttctcttg gaaagaaagt 40

<210> 356

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 356

caacatagct gggcactata gaggtttttc tcttggaaag aaagt 45

<210> 357

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 357

ctccaacgac gcagagaaac actttttctc ttggaaagaa agt 43

<210> 358

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 358

atggcgcgct ttgaggatcc tttttgaagt taccgtttt 39

<210> 359

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 359

aacacggcga ccctacaagc tttttctgag tcaaagcat 39

<210> 360

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 360

tacctgatct gtgcacggaa ctgtttttga agttaccgtt tt 42

<210> 361

<211> 48

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 361

aacacttcac tgcaagacat agaaataact ttttctgagt caaagcat 48

<210> 362

<211> 48

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 362

ttatttgtgg tgtatagaga cagtatacct ttttgaagtt accgtttt 48

<210> 363

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 363

gcatgctgca tgccataaat gtatagtttt tctgagtcaa agcat 45

<210> 364

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 364

actctgtgta tggagacaca ttggtttttg aagttaccgt ttt 43

<210> 365

<211> 56

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 365

aaaaactaac taacactggg ttatacaatt tattaatttt ttctgagtca aagcat 56

<210> 366

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 366

aaaccgttga atccagcaga aaaacttatt tttgaagtta ccgtttt 47

<210> 367

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 367

gacaccttaa tgaaaaacga cgatttcatt tttctgagtc aaagcat 47

<210> 368

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 368

ccagtgccat tcgtgctgca tttttgaagt taccgtttt 39

<210> 369

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 369

accgagcacg acaggaacga tttttctgag tcaaagcat 39

<210> 370

<211> 51

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 370

aagtataata ttaagtatgc atggacctaa ggtttttgaa gttaccgttt t 51

<210> 371

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 371

caacattgca agacattgta ttgcatttag tttttctgag tcaaagcat 49

<210> 372

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 372

agccccaaaa tgaaattccg gttgtttttg aagttaccgt ttt 43

<210> 373

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 373

accttctatg tcacgagcaa ttaagctttt tctgagtcaa agcat 45

<210> 374

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 374

atggagttaa tcatcaacat ttaccagctt tttgaagtta ccgtttt 47

<210> 375

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 375

ccgacgagcc gaaccacaac tttttctgag tcaaagcat 39

<210> 376

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 376

gtcacacaat gttgtgtatg tgttgtaatt tttgaagtta ccgtttt 47

<210> 377

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 377

gtgtgaagcc agaattgagc tagttttttc tgagtcaaag cat 43

<210> 378

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 378

ttcgagcatt ccagcagctg tttctttttg aagttaccgt ttt 43

<210> 379

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 379

tgaacaccct gtcctttgtg tgtctttttc tgagtcaaag cat 43

<210> 380

<211> 28

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 380

ctgtgtatat tgcaagacag tattggaa 28

<210> 381

<211> 28

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 381

gactcagagg aagaaaacga tgaaatag 28

<210> 382

<211> 23

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 382

agtagaaagc tcagcagacg acc 23

<210> 383

<211> 20

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 383

cgtggtgtgc atcccagcag 20

<210> 384

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 384

ggagcgaccc agaaagttac ctttttctct tggaaagaaa gt 42

<210> 385

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 385

acagttatgc acagagctgc aaactttttc tcttggaaag aaagt 45

<210> 386

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 386

tggaacaaca ttagaacagc aatacaactt tttctcttgg aaagaaagt 49

<210> 387

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 387

tgaagaaaag caaagacatc tggacaaatt tttctcttgg aaagaaagt 49

<210> 388

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 388

tcttgttgca gatcatcaag aacacgtttt tctcttggaa agaaagt 47

<210> 389

<211> 46

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 389

tagagaaacc cagctgtaat catgcttttt ctcttggaaa gaaagt 46

<210> 390

<211> 51

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 390

aatattgtaa ccttttgttg caagtgtgac tttttctctt ggaaagaaag t 51

<210> 391

<211> 45

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 391

atgcaccaaa agatgcacca aaagagtttt tgaagttacc gtttt 45

<210> 392

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 392

aactgcaatg tttcaggacc cacatttttc tgagtcaaag cat 43

<210> 393

<211> 55

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 393

aactatacat gatataatat tagaatgtgt gtactgtttt tgaagttacc gtttt 55

<210> 394

<211> 42

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 394

caagcaacag ttactgcgac gtgtttttct gagtcaaagc at 42

<210> 395

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 395

aggtatatga ctttgctttt cgggattttt tttgaagtta ccgtttt 47

<210> 396

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 396

atgcatagta tatagagatg ggaatccatt tttctgagtc aaagcat 47

<210> 397

<211> 56

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 397

tatgctgtat gtgataaatg tttaaagttt tattctattt ttgaagttac cgtttt 56

<210> 398

<211> 56

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 398

aaattagtga gtatagacat tattgttata gtttgtattt ttctgagtca aagcat 56

<210> 399

<211> 48

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 399

aaaccgttgt gtgatttgtt aattaggtgt ttttgaagtt accgtttt 48

<210> 400

<211> 46

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 400

tattaactgt caaaagccac tgtgtccttt ttctgagtca aagcat 46

<210> 401

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 401

aagcaaagat tccataatat aaggggtctt tttgaagtta ccgtttt 47

<210> 402

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 402

ggtggaccgg tcgatgtatg tttttctgag tcaaagcat 39

<210> 403

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 403

atggagatac acctacattg catgaatatt tttgaagtta ccgtttt 47

<210> 404

<211> 47

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 404

tatgttagat ttgcaaccag agacaacttt tttctgagtc aaagcat 47

<210> 405

<211> 49

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 405

gatctctact gttatgagca attaaatgac tttttgaagt taccgtttt 49

<210> 406

<211> 43

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 406

agctcagagg aggaggatga aatatttttc tgagtcaaag cat 43

<210> 407

<211> 39

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 407

gatggtccag ctggacaagc tttttgaagt taccgtttt 39

<210> 408

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 408

agaaccggac agagcccatt actttttctg agtcaaagca t 41

<210> 409

<211> 41

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 409

tctacgcttc ggttgtgcgt actttttgaa gttaccgttt t 41

<210> 410

<211> 44

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 410

aaagcacaca cgtagacatt cgtacttttt ctgagtcaaa gcat 44

<210> 411

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 411

tttggaagac ctgttaatgg gcac 24

<210> 412

<211> 24

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 412

actaggaatt gtgtgcccca tctg 24

<210> 413

<211> 16

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 413

actttctttc caagag 16

<210> 414

<211> 534

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 414

atgctttgac tcagaggatc gtaagtaact aaggactacc aggatcgtaa gtaactaagg 60

actaccagga tcgtaagtaa ctaaggacta ccaggatcgt aagtaactaa ggactaccag 120

gatcgtaagt aactaaggac taccaggatc gtaagtaact aaggactacc aggatcgtaa 180

gtaactaagg actaccagga tcgtaagtaa ctaaggacta ccaggatcgt aagtaactaa 240

ggactaccag gatcgtaagt aactaaggac taccaggatc gtaagtaact aaggactacc 300

aggatcgtaa gtaactaagg actaccagga tcgtaagtaa ctaaggacta ccaggatcgt 360

aagtaactaa ggactaccag gatcgtaagt aactaaggac taccaggatc gtaagtaact 420

aaggactacc aggatcgtaa gtaactaagg actaccagga tcgtaagtaa ctaaggacta 480

ccaggatcgt aagtaactaa ggactaccag gatcgtaagt aactaaggac tacc 534

<210> 415

<211> 534

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 415

aaaacggtaa cttcaggatc gtaagtaact aaggactacc aggatcgtaa gtaactaagg 60

actaccagga tcgtaagtaa ctaaggacta ccaggatcgt aagtaactaa ggactaccag 120

gatcgtaagt aactaaggac taccaggatc gtaagtaact aaggactacc aggatcgtaa 180

gtaactaagg actaccagga tcgtaagtaa ctaaggacta ccaggatcgt aagtaactaa 240

ggactaccag gatcgtaagt aactaaggac taccaggatc gtaagtaact aaggactacc 300

aggatcgtaa gtaactaagg actaccagga tcgtaagtaa ctaaggacta ccaggatcgt 360

aagtaactaa ggactaccag gatcgtaagt aactaaggac taccaggatc gtaagtaact 420

aaggactacc aggatcgtaa gtaactaagg actaccagga tcgtaagtaa ctaaggacta 480

ccaggatcgt aagtaactaa ggactaccag gatcgtaagt aactaaggac tacc 534

<210> 416

<211> 466

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 416

ggtagtcctt agttacttac gatcctgaat ccattgaatc ctgtgattga atccattgaa 60

tcctgtgatt gaatccattg aatcctgtga ttgaatccat tgaatcctgt gattgaatcc 120

attgaatcct gtgattgaat ccattgaatc ctgtgattga atccattgaa tcctgtgatt 180

gaatccattg aatcctgtga ttgaatccat tgaatcctgt gattgaatcc attgaatcct 240

gtgattgaat ccattgaatc ctgtgattga atccattgaa tcctgtgatt gaatccattg 300

aatcctgtga ttgaatccat tgaatcctgt gattgaatcc attgaatcct gtgattgaat 360

ccattgaatc ctgtgattga atccattgaa tcctgtgatt gaatccattg aatcctgtga 420

ttgaatccat tgaatcctgt gattgaatcc attgaatcct gtgatt 466

<210> 417

<211> 22

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 417

aatcacagga ttcaatggat tc 22

Claims (10)

1.一种***检测探针，其特征在于，包括高危型HPV16、18、31、33、35、39、45、51、52、56、58、59、66和68型中的一种或两种以上的探针；所述各高危型HPV探针均由捕获延伸探针、K型标记延伸探针和封闭探针三类探针组成；

其中，所述高危型HPV31型捕获延伸探针序列如SEQ ID No.1-6所示，所述高危型HPV31型K型标记延伸探针序列如SEQ ID No.7-20所示，所述高危型HPV31型封闭探针序列如SEQID No.21-27所示；

所述高危型HPV33型捕获延伸探针序列如SEQ ID No.28-33所示，所述高危型HPV33型K型标记延伸探针序列如SEQ ID No.34-49所示，所述高危型HPV33型封闭探针序列如SEQ IDNo.50-55所示；

所述高危型HPV35型捕获延伸探针序列如SEQ ID No.56-62所示，所述高危型HPV35型K型标记延伸探针序列如SEQ ID No.63-78所示，所述高危型HPV35型封闭探针序列如SEQ IDNo.79-82所示；

所述高危型HPV39型捕获延伸探针序列如SEQ ID No.83-90所示，所述高危型HPV39型K型标记延伸探针序列如SEQ ID No.91-112所示，所述高危型HPV39型封闭探针序列如SEQID No.113所示；

所述高危型HPV45型捕获延伸探针序列如SEQ ID No.114-121所示，所述高危型HPV45型K型标记延伸探针序列如SEQ ID No.122-139所示，所述高危型HPV45型封闭探针序列如SEQ ID No.140-146所示；

所述高危型HPV51型捕获延伸探针序列如SEQ ID No.147-153所示，所述高危型HPV51型K型标记延伸探针序列如SEQ ID No.154-173所示，所述高危型HPV51型封闭探针序列如SEQ ID No.174-175所示；

所述高危型HPV52型捕获延伸探针序列如SEQ ID No.176-178所示，所述高危型HPV52型K型标记延伸探针序列如SEQ ID No.179-194所示，所述高危型HPV52型封闭探针序列如SEQ ID No.195-204所示；

所述高危型HPV56型捕获延伸探针序列如SEQ ID No.205-208所示，所述高危型HPV56型K型标记延伸探针序列如SEQ ID No.209-228所示，所述高危型HPV56型封闭探针序列如SEQ ID No.229-234所示；

所述高危型HPV58型捕获延伸探针序列如SEQ ID No.235-238所示，所述高危型HPV58型K型标记延伸探针序列如SEQ ID No.239-255所示，所述高危型HPV58型封闭探针序列如SEQ ID No.256-262所示；

所述高危型HPV59型捕获延伸探针序列如SEQ ID No.263-267所示，所述高危型HPV59型K型标记延伸探针序列如SEQ ID No.268-289所示，所述高危型HPV59型封闭探针序列如SEQ ID No.290-292所示；

所述高危型HPV66型捕获延伸探针序列如SEQ ID No.293-298所示，所述高危型HPV66型K型标记延伸探针序列如SEQ ID No.299-316所示，所述高危型HPV66型封闭探针序列如SEQ ID No.317-321所示；

所述高危型HPV68型捕获延伸探针序列如SEQ ID No.322-329所示，所述高危型HPV68型K型标记延伸探针序列如SEQ ID No.330-349所示，所述高危型HPV68型封闭探针序列如SEQ ID No.350-352所示；

所述高危型HPV18型捕获延伸探针序列如SEQ ID No.353-357所示，所述高危型HPV18型K型标记延伸探针序列如SEQ ID No.358-379所示，所述高危型HPV18型封闭探针序列如SEQ ID No.380-383所示；

所述高危型HPV16型捕获延伸探针序列如SEQ ID No.384-390所示，所述高危型HPV16型K型标记延伸探针序列如SEQ ID No.391-410所示，所述高危型HPV16型封闭探针序列如SEQ ID No.411-412所示。

2.权利要求1所述探针在制备检测***试剂盒中的应用。

3.一种检测***的试剂盒，其特征在于，包括权利要求1所述探针。

4.根据权利要求3所述试剂盒，其特征在于，还包括信号放大前体、信号放大体、碱性磷酸酶（AP）标记的报告探针、化学发光底物以及偶联有通用探针的载体中的一种或两种以上；

其中，所述通用探针与权利要求1中所述捕获延伸探针的部分序列互补配对；

所述信号放大前体包括放大前体前置引导区序列和多次重复的放大体前置引导区互补序列；

所述信号放大体包括放大体前置引导区序列和多次重复的报告探针互补序列；

所述放大前体前置引导区序列与权利要求1中所述K型标记延伸探针部分序列互补配对；

所述放大体前置引导区序列与所述多次重复的放大体前置引导区互补序列互补配对；

所述碱性磷酸酶标记的报告探针与所述多次重复的报告探针互补序列互补配对。

5.根据权利要求4所述试剂盒，其特征在于，所述载体为磁珠或细胞培养板。

6.根据权利要求4所述试剂盒，其特征在于，所述通用探针的序列如SEQ ID No.413所示。

7.根据权利要求4所述试剂盒，其特征在于，所述化学发光底物为金刚烷衍生物。

8.根据权利要求4所述试剂盒，其特征在于，所述多次重复为20次重复。

9.根据权利要求4所述试剂盒，其特征在于，所述通用探针与权利要求1中所述捕获延伸探针的部分序列互补配对，其中所述部分序列为权利要求1中所述捕获延伸探针的3’端至5’端方向的16bp序列。

10.根据权利要求4所述试剂盒，其特征在于，所述放大前体前置引导区序列与权利要求1中所述K型标记延伸探针部分序列互补配对，其中所述部分序列为权利要求1中所述K型标记延伸探针的3’端至5’端方向的14bp序列。

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