CN105962378A - Preparation method of active lactobacillus preparation containing hericium polysaccharides - Google Patents
Preparation method of active lactobacillus preparation containing hericium polysaccharides Download PDFInfo
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- CN105962378A CN105962378A CN201610312050.2A CN201610312050A CN105962378A CN 105962378 A CN105962378 A CN 105962378A CN 201610312050 A CN201610312050 A CN 201610312050A CN 105962378 A CN105962378 A CN 105962378A
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- hericium erinaceus
- erinaceus polysaccharide
- lactic acid
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- 241000186660 Lactobacillus Species 0.000 title claims abstract description 51
- 229940039696 lactobacillus Drugs 0.000 title claims abstract description 51
- 241000123222 Hericium Species 0.000 title abstract 5
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a preparation method of an active lactobacillus preparation containing hericium polysaccharides. The preparation method includes the steps of (1) preparing the hericium polysaccharides; (2) preparing an active lactobacillus powdered preparation; (3) preparing sea-buckthorn powder, apple peel powder and peanut coat powder; (4) preparing a finished product. Gastrointestinal tract system disease prevention and treatment functions of the hericium polysaccharides are made full use of, essence parts of hericium are extracted, a beneficial intestinal flora is led in through manual intervention by the technology of combined co-fermentation of various probiotics, probiotics beneficial to normal flora proliferation of a human body are added, material basis is provided for continuous proliferation of intestinal bacteria, the sea-buckthorn powder, the apple peel powder and the peanut coat powder are particularly added, and the active lactobacillus preparation has fine healthcare effects and no toxic and side effects.
Description
Technical field
The present invention relates to health product technology field, particularly relate to a kind of active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide
Preparation method.
Background technology
Polysaccharide is the living polymer compound that a class has 3-D solid structure, is the important composition of cell receptor,
Identify and transmission information, it is achieved the physiological process of living things catalysis and material conversion plays very important effect.Hericium erinaceus (Bull. Ex Fr.) Pers., from
Being exactly famous dietotherapeutic fungus since Gu, can not only cook delicious food, be again that dyspepsia is cured in auxiliary treatment, stomach is burst
Infections, the health food of the digestive system disease such as duodenal ulcer, esophageal carcinoma, gastric cancer, and there is good tonic effect.Mesh
Before at home and abroad, Hericium erinaceus (Bull. Ex Fr.) Pers. is the most extensively applied to curing the disease of gi system and tumor.Research shows, Hericium erinaceus (Bull. Ex Fr.) Pers. is many
Sugar is effect position that it is main, is the elite place of Hericium erinaceus (Bull. Ex Fr.) Pers., has: 1) suppression tumor growth；2) facilitating digestion road ulcer
Reparation with various chronic gastritiss；3) treatment hepatitis；4) improve Leukocytes after Chemotherapy quantity, regulate body's immunity.Clinical
Use result it has been proved that Hericium Erinaceus Polysaccharide is without any toxicity, side effect.Lactobacillus is the main of human body intestinal canal normal flora
Microorganism, has a lot of beneficial effect to human body, including exclusion, suppresses the nutrition such as external pathogenic bacterium, offer vitamin, produces and form sediment
Powder enzyme, protease etc. contribute to the enzyme of digestion, decompose poisonous or carcinogen (nitrous ammonium), produce organic acid, reduce intestinal
PH and the normal creepage of gastrointestinal functions of promotion intestinal, stimulate the immune system of body and improve immunity.A large amount of at intestinal of Lactobacillus
Breeding, can treat pseudomembranous enteritis (and using antibiotic to cause because of a large amount of), treatment chronic diarrhea and constipation, reduce gallbladder solid
Alcohol, the generation of prophylaxis of cancer.Prebiotics is original in referring to selectively promote human body intestinal canal, and one or more are useful carefully
The material of bacterium (probiotic bacteria) growth and breeding, is increased by the breeding of probiotics, suppresses harmful bacterial growth, thus reaches to adjust intestinal
Road flora, promotes the purpose of body health.Prebiotics, not by human consumption and absorption, common are oligomeric xylose, lactulose, sugarcane
Sugar oligosaccharide, cotton seed oligosaccharide, dextrinosan, Semen Maydis oligosaccharide, soybean oligo saccharide etc..
Application publication number CN 103830279 A(application number 201310736926. 2) Chinese patent literature disclose one
Plant selenium-rich lactobacillus preparation production method and selenium-rich lactobacillus preparation, the selenium-rich lactobacillus preparation of this invention, its main component
Being enriched in the lactic acid bacteria of organic selenium, described organic selenium is to convert inorganic selenium by lactic acid bacteria fermentation and be enriched in thalline, often
In g of formulation, Se content is 4-12 milligram.Application publication number CN 104721237 A(application number 201310714553.9) China
Patent documentation discloses a kind of lactobacillus micro-ecological preparation in intestinal tract disease medicine, and it includes the group of following quality proportioning
Point: inactivation lactobacillus cell and lactic acid bacteria fermentation product 15-55 part, wherein inactivation lactobacillus cell 108-109/gram, figuration
Agent 32-48 part, described lactic acid bacteria selects white bacillus acidophilus, lactobacillus lactis, streptococcus thermophilus, Pediococcus pentosaceus, lactic acid sheet ball
One or more in bacterium, Lactobacillus bulgaricus, bacillus bifidus, described excipient is 60-100 mesh maize cob meal and heavy
The mixture of the mixture of shallow lake silicon dioxide, corn starch and precipitated silica or maize cob meal, corn starch and precipitation
The mixture of silicon dioxide three.Application publication number CN 104721236 A(application number 201310713047.8) Chinese patent
Document discloses the preparation method of a kind of lactobacillus micro-ecological preparation in intestinal tract disease medicine, and it comprises the following steps:
1) choose lactic acid bacteria fermentation to cultivate；2) in fermentation liquid, the protective agent that mass fraction is 0. 1-0. 5% is added after fermentation ends；
3) inactivation, centrifugal collection thalline；4) concentrated supernatant, adds excipient and glucose or lactose, is dried, makes residual moisture be less than
15%；5) thalline that step 3) obtains is mixed with the product of step 4).
At present, the variety type of the lactobacillus preparation on market is less, and function is more single.
Summary of the invention
For the deficiencies in the prior art, it is an object of the invention to provide a kind of Lactobacillus system containing Hericium Erinaceus Polysaccharide
The preparation method of agent.The present invention makes full use of Hericium Erinaceus Polysaccharide to gastrointestinal system prevention and treatment of diseases effect, extraction monkey
Head mushroom elite position, uses multiple probiotic combination fermentation technique altogether, and manual intervention introduces intestinal beneficial flora, is added with and benefits
The prebiotics of Body normal flora propagation, the continuous proliferation for intestinal bacteria provides material base, is especially added with Fructus Hippophae powder, Herba Marsileae Quadrifoliae
Fruit peel powder and Testa arachidis hypogaeae powder, have good health-care effect, without any toxicity, side effect.
The technical scheme is that
The preparation method of a kind of active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide, is carried out as steps described below: (1) Hericium erinaceus (Bull. Ex Fr.) Pers. is many
The preparation of sugar；(2) preparation of Lactobacillus powder preparation；(3) Fructus Hippophae powder, apple peel powder and the preparation of Testa arachidis hypogaeae powder；(4)
Prepare finished product.
The preparation method of a kind of active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide, the preparation of described Hericium Erinaceus Polysaccharide is divided into
Five steps below: the pretreatment of (1) Hericium erinaceus (Bull. Ex Fr.) Pers. raw material；(2) dissolution of Hericium Erinaceus Polysaccharide；(3) little molecule is removed in ultrafiltration；(4)
The precipitate with ethanol of Hericium Erinaceus Polysaccharide；(5) vacuum lyophilization；
(1) pretreatment of Hericium erinaceus (Bull. Ex Fr.) Pers. raw material: take and be dried the hericium erinaceus fruiting body to constant weight, pulverizes, is placed in the 65 of 6-13 times of volume
～90% to soak 20～28 h(in ethanol solution preferred, be placed in the 70-85% ethanol solution of 8～11 times of volumes immersion 23～
26 h；More preferably, be placed in 80% ethanol solution of 9 times of volumes and soak 25 h), with remove fatty, part single (few) is sugared and
Fat-soluble pigment, air-dries at residue cool place；
(2) dissolution of Hericium Erinaceus Polysaccharide: after residue air-dries, the selective extraction time is 1.2～1.8 h, and temperature is 70～90 DEG C, water
Material ratio is 24～28mL/g(v/w), 110 mesh filtered through gauze, filtering residue repeats to extract 3 times；
(3) little molecule is removed in ultrafiltration: step (2) gained filtrate merged, and using ultrafilter membrane molecular weight is the hollow cellulose of 10,000
Film carries out ultrafiltration, removes the small-molecule substance of less than 10,000；
(4) precipitate with ethanol of Hericium Erinaceus Polysaccharide: add dehydrated alcohol in the extracting solution after ultrafiltration concentration, until the ethanol terminal of solution is dense
Degree is 65～85%(preferably, until the ethanol endpoint concentration of solution is 70～80%；More preferably, until the ethanol of solution
Endpoint concentration is 75%), 4 DEG C stand 24 h, centrifugal, take sediment fraction, lyophilization；
(5) vacuum lyophilization: step (4) gained is positioned at 20 DEG C freezing 4h, then proceeds at 80 DEG C freezing
12h, then proceeds to vacuum freeze drier and is dried, obtain Hericium Erinaceus Polysaccharide.
A kind of preparation method of the active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide, described Lactobacillus powder preparation
Be prepared as:
(1) dispensing: raw material milk is warming up to 45～55 DEG C, addition 0.15～the low methoxyl pectin of 0.25% and 9～the white sugar of 15%,
Fully mixing；
(2) homogenizing: feed liquid step (1) prepared carries out homogenizing at 15～30 Mpa, under the conditions of 60～75 DEG C；
(3) sterilizing: the feed liquid after homogenizing is continued 8～12min at 95 DEG C, is cooled to 40 DEG C～45 DEG C；
(4) inoculation fermentation: by fermentating liquid volume 4～the amount inoculating compound bacterium agent of 7%, temperature 45 C stands constant temperature culture 5～7 h,
When acidity reaches 70～75 ° of T, product is cooled to cold preservation after-ripening 15～26 h at 2～6 DEG C, obtains curdy cultured milk；
(5) it is dried, makes powder: be dried by step (4) gained curdy cultured milk 50 DEG C of spray dryers of employing, prepare activity
Lactic acid bacteria powder preparation.
A kind of preparation method of the active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide, described Lactobacillus powder preparation
Preparation process (4) described in composite bacteria agent capable by streptococcus thermophilus, Bulgarian Lactobacillus, lactobacillus casei, bacillus bifidus
In two kinds or three kinds or four kinds combine.
A kind of preparation method of the active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide, being prepared as of described Fructus Hippophae powder: take sand
Spine, squeezes extracting juice, filters, takes the supernatant, concentrates 3 hours in 50-60 DEG C, is spray-dried, obtains Fructus Hippophae powder；Described apple peel powder
Be prepared as: Pericarpium Mali pumilae be dry, pulverize, sieves, obtain apple peel powder；Being prepared as of described Testa arachidis hypogaeae powder: take Testa arachidis hypogaeae, adds
The water of 5-8 times, decocts 1.5 hours, takes the supernatant, be condensed into cream, is dried, obtains Testa arachidis hypogaeae powder.
The preparation method of a kind of active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide, described finished product of preparing is: weigh respectively
Hericium Erinaceus Polysaccharide, Fructus Hippophae powder, Lactobacillus powder preparation, apple peel powder, prebiotics, Testa arachidis hypogaeae powder and starch, shared weight
Amount part is respectively as follows: Hericium Erinaceus Polysaccharide 18-26, Fructus Hippophae powder 5-9, Lactobacillus dry powder 20-25, Pericarpium Mali pumilae 3-7, prebiotics 1.5-
3, Testa arachidis hypogaeae powder 2-6, starch 43-55, make tablet through dry method.
A kind of preparation method of the active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide, described prebiotics be soybean oligo saccharide and
One in oligomeric xylose or two kinds of combinations.
The preparation method of a kind of active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide, described starch is wheaten starch, Semen Maydis
Starch, the one in sweet potato starch, the combination of two or three.
Product of the present invention carries and instant.In addition, the excellent results of the present invention is also manifested by:
(1) product of the present invention be provided simultaneously with the nutrition health-care functions of Hericium Erinaceus Polysaccharide, the prebiotic effect of active lactic acid bacteria preparation and
The selective proliferative effect of prebiotics, adds Fructus Hippophae powder, apple peel powder and Testa arachidis hypogaeae powder, has good health-care effect, it is provided that
A kind of there is nourishing the stomach stomach invigorating, there is the multifunctional health compound formulation of continuous proliferation effect, not only expanded Hericium erinaceus (Bull. Ex Fr.) Pers.
Utilization ways, improves the added value of Hericium erinaceus (Bull. Ex Fr.) Pers., too increases the variety type of lactobacillus preparation, there are no this in the market
Product.
(2) suitable population of the present invention is extensive, especially to gastrointestinal dysfunction persons such as dyspepsia, low, the constipation of appetite, with
And hypoimmunity, rhythm of life are nervous, diet does not has rule, is susceptible to suffer from the professional population of gastrointestinal disease, better, have good
Good Social benefit and economic benefit.
Detailed description of the invention
Describe technical scheme in detail below in conjunction with embodiment and experimental example, but protection domain is not limited to this.
The preparation method of the embodiment 1 one kinds active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide, is carried out as steps described below:
(1) preparation of Hericium erinaceus (Bull. Ex Fr.) Pers. Polysaccharides；(2) preparation of Lactobacillus powder preparation；(3) Fructus Hippophae powder, apple peel powder and Testa arachidis hypogaeae
The preparation of powder；(4) finished product is prepared.
The preparation of described Hericium Erinaceus Polysaccharide is divided into following five steps: the pretreatment of (1) Hericium erinaceus (Bull. Ex Fr.) Pers. raw material；(2) Hericium erinaceus (Bull. Ex Fr.) Pers.
The dissolution of polysaccharide；(3) little molecule is removed in ultrafiltration；(4) precipitate with ethanol of Hericium Erinaceus Polysaccharide；(5) vacuum lyophilization；
(1) pretreatment of Hericium erinaceus (Bull. Ex Fr.) Pers. raw material: take and be dried the hericium erinaceus fruiting body to constant weight, pulverizes, is placed in 65% second of 6 times of volumes
Alcohol solution for soaking 20 h, to remove fatty, part single (few) sugar and fat-soluble pigment, air-dries at residue cool place；
(2) dissolution of Hericium Erinaceus Polysaccharide: after residue air-dries, the selective extraction time is 1.2 h, and temperature is 70 DEG C, and ratio of water to material is
24mL/g(v/w), 110 mesh filtered through gauze, filtering residue repeats to extract 3 times；
(3) little molecule is removed in ultrafiltration: step (2) gained filtrate merged, and using ultrafilter membrane molecular weight is the hollow cellulose of 10,000
Film carries out ultrafiltration, removes the small-molecule substance of less than 10,000；
(4) precipitate with ethanol of Hericium Erinaceus Polysaccharide: add dehydrated alcohol in the extracting solution after ultrafiltration concentration, until the ethanol terminal of solution is dense
Degree is 65%, and 4 DEG C stand 24 h, centrifugal, take sediment fraction, lyophilization；
(5) vacuum lyophilization: step (4) gained is positioned at 20 DEG C freezing 4h, then proceeds at 80 DEG C freezing
12h, then proceeds to vacuum freeze drier and is dried, obtain Hericium Erinaceus Polysaccharide.
Being prepared as of described Lactobacillus powder preparation:
(1) dispensing: raw material milk is warming up to 45 DEG C, adds low methoxyl pectin and the white sugar of 9% of 0.15%, fully mixes；
(2) homogenizing: feed liquid step (1) prepared carries out homogenizing at 15 Mpa, under the conditions of 60 DEG C；
(3) sterilizing: the feed liquid after homogenizing is continued 8min at 95 DEG C, is cooled to 40 DEG C；
(4) inoculation fermentation: (used by the present embodiment, composite bacteria agent capable is by thermophilus as the amount inoculating compound bacterium agent of fermentating liquid volume 4%
Bacterium and bacillus bifidus equal proportion combine), temperature 45 C stands constant temperature culture 5 h, when acidity reaches 70 ° of T, by product
Cool to cold preservation after-ripening 15 h at 2 DEG C, obtain curdy cultured milk；
(5) it is dried, makes powder: be dried by step (4) gained curdy cultured milk 50 DEG C of spray dryers of employing, prepare activity
Lactic acid bacteria powder preparation.
Being prepared as of described Fructus Hippophae powder: take Fructus Hippophae, squeezes extracting juice, filters, takes the supernatant, concentrates 3 hours in 50 DEG C, spray
Mist is dried, and obtains Fructus Hippophae powder；Being prepared as of described apple peel powder: be dry, pulverize by Pericarpium Mali pumilae, sieves, and obtains apple peel powder；Described flower
Being prepared as of raw clothing powder: take Testa arachidis hypogaeae, add the water of 5 times, decoct 1.5 hours, take the supernatant, be condensed into cream, is dried, obtains flower
Raw clothing powder.
Described finished product of preparing is: weigh Hericium Erinaceus Polysaccharide, Fructus Hippophae powder, Lactobacillus powder preparation, Pericarpium Mali pumilae respectively
Powder, prebiotics, Testa arachidis hypogaeae powder and starch, shared weight portion is respectively (every part is 800g): Hericium Erinaceus Polysaccharide 18 parts, Fructus Hippophae powder 5
Part, 20 parts of Lactobacillus dry powder, apple peel powder 3 parts, prebiotics (prebiotics used by the present embodiment is soybean oligo saccharide) 1.5
Part, 2 parts of Testa arachidis hypogaeae powder, starch (starch used by the present embodiment is wheaten starch) 43 parts, make tablet, every tablet weight through dry method
200mg。
Gained of the present invention contains the instructions of taking of the active lactic acid bacteria preparation of Hericium Erinaceus Polysaccharide: oral, be grown up one time 2,
3 times on the one；Take by half amount of adult more than 5 one full year of life, below 15 one full year of life.
The preparation method of the embodiment 2 one kinds active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide, it is characterised in that under according to
State step to carry out: the preparation of (1) Hericium erinaceus (Bull. Ex Fr.) Pers. Polysaccharides；(2) preparation of Lactobacillus powder preparation；(3) Fructus Hippophae powder, Pericarpium Mali pumilae
Powder and the preparation of Testa arachidis hypogaeae powder；(4) finished product is prepared.
The preparation of described Hericium Erinaceus Polysaccharide is divided into following five steps: the pretreatment of (1) Hericium erinaceus (Bull. Ex Fr.) Pers. raw material；(2) Hericium erinaceus (Bull. Ex Fr.) Pers.
The dissolution of polysaccharide；(3) little molecule is removed in ultrafiltration；(4) precipitate with ethanol of Hericium Erinaceus Polysaccharide；(5) vacuum lyophilization；
(1) pretreatment of Hericium erinaceus (Bull. Ex Fr.) Pers. raw material: take and be dried the hericium erinaceus fruiting body to constant weight, pulverizes, is placed in 90% second of 13 times of volumes
Alcohol solution for soaking 28 h, to remove fatty, part single (few) sugar and fat-soluble pigment, air-dries at residue cool place；
(2) dissolution of Hericium Erinaceus Polysaccharide: after residue air-dries, the selective extraction time is 1.8 h, and temperature is 90 DEG C, and ratio of water to material is
28mL/g(v/w), 110 mesh filtered through gauze, filtering residue repeats to extract 3 times；
(3) little molecule is removed in ultrafiltration: step (2) gained filtrate merged, and using ultrafilter membrane molecular weight is the hollow cellulose of 10,000
Film carries out ultrafiltration, removes the small-molecule substance of less than 10,000；
(4) precipitate with ethanol of Hericium Erinaceus Polysaccharide: add dehydrated alcohol in the extracting solution after ultrafiltration concentration, until the ethanol terminal of solution is dense
Degree is 85%, and 4 DEG C stand 24 h, centrifugal, take sediment fraction, lyophilization；
(5) vacuum lyophilization: step (4) gained is positioned at 20 DEG C freezing 4h, then proceeds at 80 DEG C freezing
12h, then proceeds to vacuum freeze drier and is dried, obtain Hericium Erinaceus Polysaccharide.
Being prepared as of described Lactobacillus powder preparation:
(1) dispensing: raw material milk is warming up to 55 DEG C, adds low methoxyl pectin and the white sugar of 15% of 0.25%, fully mixes；
(2) homogenizing: feed liquid step (1) prepared carries out homogenizing at 30 Mpa, under the conditions of 75 DEG C；
(3) sterilizing: the feed liquid after homogenizing is continued 12min at 95 DEG C, is cooled to 45 DEG C；
(4) inoculation fermentation: (used by the present embodiment, composite bacteria agent capable is by Bulgaria as the amount inoculating compound bacterium agent of fermentating liquid volume 7%
In lactobacillus and bacillus bifidus, the ratio according to 1:1.5 combines), temperature 45 C stands constant temperature culture 7 h, treats that acidity reaches
During to 75 ° of T, product is cooled to cold preservation after-ripening 26 h at 6 DEG C, obtains curdy cultured milk；
(5) it is dried, makes powder: be dried by step (4) gained curdy cultured milk 50 DEG C of spray dryers of employing, prepare activity
Lactic acid bacteria powder preparation.
Being prepared as of described Fructus Hippophae powder: take Fructus Hippophae, squeezes extracting juice, filters, takes the supernatant, concentrates 3 hours in 60 DEG C, spray
Mist is dried, and obtains Fructus Hippophae powder；Being prepared as of described apple peel powder: be dry, pulverize by Pericarpium Mali pumilae, sieves, and obtains apple peel powder；Described flower
Being prepared as of raw clothing powder: take Testa arachidis hypogaeae, add the water of 8 times, decoct 1.5 hours, take the supernatant, be condensed into cream, is dried, obtains flower
Raw clothing powder.
Described finished product of preparing is: weigh Hericium Erinaceus Polysaccharide, Fructus Hippophae powder, Lactobacillus powder preparation, Pericarpium Mali pumilae respectively
Powder, prebiotics, Testa arachidis hypogaeae powder and starch, shared weight portion is respectively (every part is 800g): Hericium Erinaceus Polysaccharide 26 parts, Fructus Hippophae powder 9
Part, 25 parts of Lactobacillus dry powder, apple peel powder 7 parts, prebiotics (prebiotics used by the present embodiment is oligomeric xylose) 3 parts, flower
Raw 6 parts of clothing powder, starch (starch used by the present embodiment is sweet potato starch) 55 parts, make tablet, every tablet weight 200mg through dry method.
The preparation method of the embodiment 3 one kinds active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide, it is characterised in that under according to
State step to carry out: the preparation of (1) Hericium erinaceus (Bull. Ex Fr.) Pers. Polysaccharides；(2) preparation of Lactobacillus powder preparation；(3) Fructus Hippophae powder, Pericarpium Mali pumilae
Powder and the preparation of Testa arachidis hypogaeae powder；(4) finished product is prepared.
The preparation of described Hericium Erinaceus Polysaccharide is divided into following five steps: the pretreatment of (1) Hericium erinaceus (Bull. Ex Fr.) Pers. raw material；(2) Hericium erinaceus (Bull. Ex Fr.) Pers.
The dissolution of polysaccharide；(3) little molecule is removed in ultrafiltration；(4) precipitate with ethanol of Hericium Erinaceus Polysaccharide；(5) vacuum lyophilization；
(1) pretreatment of Hericium erinaceus (Bull. Ex Fr.) Pers. raw material: take and be dried the hericium erinaceus fruiting body to constant weight, pulverizes, is placed in 70% second of 8 times of volumes
Alcohol solution for soaking 23 h, to remove fatty, part single (few) sugar and fat-soluble pigment, air-dries at residue cool place；
(2) dissolution of Hericium Erinaceus Polysaccharide: after residue air-dries, the selective extraction time is 1.2 h, and temperature is 70 DEG C, and ratio of water to material is
24mL/g(v/w), 110 mesh filtered through gauze, filtering residue repeats to extract 3 times；
(3) little molecule is removed in ultrafiltration: step (2) gained filtrate merged, and using ultrafilter membrane molecular weight is the hollow cellulose of 10,000
Film carries out ultrafiltration, removes the small-molecule substance of less than 10,000；
(4) precipitate with ethanol of Hericium Erinaceus Polysaccharide: add dehydrated alcohol in the extracting solution after ultrafiltration concentration, until the ethanol terminal of solution is dense
Degree is 70%, and 4 DEG C stand 24 h, centrifugal, take sediment fraction, lyophilization；
(5) vacuum lyophilization: step (4) gained is positioned at 20 DEG C freezing 4h, then proceeds at 80 DEG C freezing
12h, then proceeds to vacuum freeze drier and is dried, obtain Hericium Erinaceus Polysaccharide.
Being prepared as of described Lactobacillus powder preparation:
(1) dispensing: raw material milk is warming up to 45 DEG C, adds low methoxyl pectin and the white sugar of 9% of 0.15%, fully mixes；
(2) homogenizing: feed liquid step (1) prepared carries out homogenizing at 15Mpa, under the conditions of 60 DEG C；
(3) sterilizing: the feed liquid after homogenizing is continued 8min at 95 DEG C, is cooled to 40 DEG C；
(4) inoculation fermentation: (used by the present embodiment, composite bacteria agent capable is by Bulgaria as the amount inoculating compound bacterium agent of fermentating liquid volume 4%
In lactobacillus, lactobacillus casei and bacillus bifidus, the ratio according to 1:1.2:1.5 combines), temperature 45 C stands constant temperature
Cultivate 5h, when acidity reaches 70 ° of T, product is cooled to cold preservation after-ripening 15 h at 2 DEG C, obtains curdy cultured milk；
(5) it is dried, makes powder: be dried by step (4) gained curdy cultured milk 50 DEG C of spray dryers of employing, prepare activity
Lactic acid bacteria powder preparation.
Being prepared as of described Fructus Hippophae powder: take Fructus Hippophae, squeezes extracting juice, filters, takes the supernatant, concentrates 3 hours in 50 DEG C, spray
Mist is dried, and obtains Fructus Hippophae powder；Being prepared as of described apple peel powder: be dry, pulverize by Pericarpium Mali pumilae, sieves, and obtains apple peel powder；Described flower
Being prepared as of raw clothing powder: take Testa arachidis hypogaeae, add the water of 5 times, decoct 1.5 hours, take the supernatant, be condensed into cream, is dried, obtains flower
Raw clothing powder.
Described finished product of preparing is: weigh Hericium Erinaceus Polysaccharide, Fructus Hippophae powder, Lactobacillus powder preparation, Pericarpium Mali pumilae respectively
Powder, prebiotics, Testa arachidis hypogaeae powder and starch, shared weight portion is respectively (every part is 800g): Hericium Erinaceus Polysaccharide 18 parts, Fructus Hippophae powder 5
Part, 20 parts of Lactobacillus dry powder, apple peel powder 3 parts, (prebiotics used by the present embodiment is soybean oligo saccharide and oligomeric to prebiotics
Xylose is combined in the ratio of 1.4:1.8) 1.5 parts, 2 parts of Testa arachidis hypogaeae powder, (starch used by the present embodiment is corn starch to starch
Combine in the ratio of 1.5:2 with sweet potato starch) 43 parts, make tablet, every tablet weight 200mg through dry method.
The preparation method of the embodiment 4 one kinds active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide, it is characterised in that under according to
State step to carry out: the preparation of (1) Hericium erinaceus (Bull. Ex Fr.) Pers. Polysaccharides；(2) preparation of Lactobacillus powder preparation；(3) Fructus Hippophae powder, Pericarpium Mali pumilae
Powder and the preparation of Testa arachidis hypogaeae powder；(4) finished product is prepared.
The preparation of described Hericium Erinaceus Polysaccharide is divided into following five steps: the pretreatment of (1) Hericium erinaceus (Bull. Ex Fr.) Pers. raw material；(2) Hericium erinaceus (Bull. Ex Fr.) Pers.
The dissolution of polysaccharide；(3) little molecule is removed in ultrafiltration；(4) precipitate with ethanol of Hericium Erinaceus Polysaccharide；(5) vacuum lyophilization；
(1) pretreatment of Hericium erinaceus (Bull. Ex Fr.) Pers. raw material: take and be dried the hericium erinaceus fruiting body to constant weight, pulverizes, is placed in 85% second of 11 times of volumes
Alcohol solution for soaking 26 h, to remove fatty, part single (few) sugar and fat-soluble pigment, air-dries at residue cool place；
(2) dissolution of Hericium Erinaceus Polysaccharide: after residue air-dries, the selective extraction time is 1.8 h, and temperature is 90 DEG C, and ratio of water to material is
28mL/g(v/w), 110 mesh filtered through gauze, filtering residue repeats to extract 3 times；
(3) little molecule is removed in ultrafiltration: step (2) gained filtrate merged, and using ultrafilter membrane molecular weight is the hollow cellulose of 10,000
Film carries out ultrafiltration, removes the small-molecule substance of less than 10,000；
(4) precipitate with ethanol of Hericium Erinaceus Polysaccharide: add dehydrated alcohol in the extracting solution after ultrafiltration concentration, until the ethanol terminal of solution is dense
Degree is 80%, and 4 DEG C stand 24 h, centrifugal, take sediment fraction, lyophilization；
(5) vacuum lyophilization: step (4) gained is positioned at 20 DEG C freezing 4h, then proceeds at 80 DEG C freezing
12h, then proceeds to vacuum freeze drier and is dried, obtain Hericium Erinaceus Polysaccharide.
Being prepared as of described Lactobacillus powder preparation:
(1) dispensing: raw material milk is warming up to 55 DEG C, adds low methoxyl pectin and the white sugar of 15% of 0.25%, fully mixes；
(2) homogenizing: feed liquid step (1) prepared carries out homogenizing at 30 Mpa, under the conditions of 75 DEG C；
(3) sterilizing: the feed liquid after homogenizing is continued 12min at 95 DEG C, is cooled to 45 DEG C；
(4) inoculation fermentation: (used by the present embodiment, composite bacteria agent capable is by thermophilus as the amount inoculating compound bacterium agent of fermentating liquid volume 7%
In bacterium, lactobacillus casei and bacillus bifidus, the ratio according to 1.2:1.6:1.5 combines), temperature 45 C stands constant temperature culture 7
H, when acidity reaches 75 ° of T, cools to product cold preservation after-ripening 26 h at 6 DEG C, obtains curdy cultured milk；
(5) it is dried, makes powder: be dried by step (4) gained curdy cultured milk 50 DEG C of spray dryers of employing, prepare activity
Lactic acid bacteria powder preparation.
Being prepared as of described Fructus Hippophae powder: take Fructus Hippophae, squeezes extracting juice, filters, takes the supernatant, concentrates 3 hours in 60 DEG C, spray
Mist is dried, and obtains Fructus Hippophae powder；Being prepared as of described apple peel powder: be dry, pulverize by Pericarpium Mali pumilae, sieves, and obtains apple peel powder；Described flower
Being prepared as of raw clothing powder: take Testa arachidis hypogaeae, add the water of 8 times, decoct 1.5 hours, take the supernatant, be condensed into cream, is dried, obtains flower
Raw clothing powder.
Described finished product of preparing is: weigh Hericium Erinaceus Polysaccharide, Fructus Hippophae powder, Lactobacillus powder preparation, Pericarpium Mali pumilae respectively
Powder, prebiotics, Testa arachidis hypogaeae powder and starch, shared weight portion is respectively (every part is 800g): Hericium Erinaceus Polysaccharide 26 parts, Fructus Hippophae powder 9
Part, 25 parts of Lactobacillus dry powder, apple peel powder 7 parts, (prebiotics used by the present embodiment is soybean oligo saccharide and oligomeric to prebiotics
Xylose is combined in the ratio of 1:1.2) 3 parts, 6 parts of Testa arachidis hypogaeae powder, (starch used by the present embodiment is wheaten starch and ground to starch
Melon starch is combined in the ratio of 1.5:1.8) 55 parts, make tablet, every tablet weight 200mg through dry method.
The preparation method of the embodiment 5 one kinds active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide, it is characterised in that under according to
State step to carry out: the preparation of (1) Hericium erinaceus (Bull. Ex Fr.) Pers. Polysaccharides；(2) preparation of Lactobacillus powder preparation；(3) Fructus Hippophae powder, Pericarpium Mali pumilae
Powder and the preparation of Testa arachidis hypogaeae powder；(4) finished product is prepared.
The preparation of described Hericium Erinaceus Polysaccharide is divided into following five steps: the pretreatment of (1) Hericium erinaceus (Bull. Ex Fr.) Pers. raw material；(2) Hericium erinaceus (Bull. Ex Fr.) Pers.
The dissolution of polysaccharide；(3) little molecule is removed in ultrafiltration；(4) precipitate with ethanol of Hericium Erinaceus Polysaccharide；(5) vacuum lyophilization；
(1) pretreatment of Hericium erinaceus (Bull. Ex Fr.) Pers. raw material: take and be dried the hericium erinaceus fruiting body to constant weight, pulverizes, is placed in 80% second of 9 times of volumes
Alcohol solution for soaking 25 h, to remove fatty, part single (few) sugar and fat-soluble pigment, air-dries at residue cool place；
(2) dissolution of Hericium Erinaceus Polysaccharide: after residue air-dries, the selective extraction time is 1.8 h, and temperature is 90 DEG C, and ratio of water to material is
28mL/g(v/w), 110 mesh filtered through gauze, filtering residue repeats to extract 3 times；
(3) little molecule is removed in ultrafiltration: step (2) gained filtrate merged, and using ultrafilter membrane molecular weight is the hollow cellulose of 10,000
Film carries out ultrafiltration, removes the small-molecule substance of less than 10,000；
(4) precipitate with ethanol of Hericium Erinaceus Polysaccharide: add dehydrated alcohol in the extracting solution after ultrafiltration concentration, until the ethanol terminal of solution is dense
Degree is 75%, and 4 DEG C stand 24 h, centrifugal, take sediment fraction, lyophilization；
(5) vacuum lyophilization: step (4) gained is positioned at 20 DEG C freezing 4h, then proceeds at 80 DEG C freezing
12h, then proceeds to vacuum freeze drier and is dried, obtain Hericium Erinaceus Polysaccharide.
Being prepared as of described Lactobacillus powder preparation:
(1) dispensing: raw material milk is warming up to 55 DEG C, adds low methoxyl pectin and the white sugar of 15% of 0.25%, fully mixes；
(2) homogenizing: feed liquid step (1) prepared carries out homogenizing at 30 Mpa, under the conditions of 75 DEG C；
(3) sterilizing: the feed liquid after homogenizing is continued 12min at 95 DEG C, is cooled to 45 DEG C；
(4) inoculation fermentation: (used by the present embodiment, composite bacteria agent capable is by thermophilus as the amount inoculating compound bacterium agent of fermentating liquid volume 7%
Bacterium, Bulgarian Lactobacillus, lactobacillus casei, bacillus bifidus combine according to the ratio of 1.3:1.5:1.2:1.6), temperature
Spend 45 DEG C and stand constant temperature culture 7 h, when acidity reaches 75 ° of T, product is cooled to cold preservation after-ripening 26 h at 6 DEG C, is coagulated
Emulsus cultured milk；
(5) it is dried, makes powder: be dried by step (4) gained curdy cultured milk 50 DEG C of spray dryers of employing, prepare activity
Lactic acid bacteria powder preparation.
Being prepared as of described Fructus Hippophae powder: take Fructus Hippophae, squeezes extracting juice, filters, takes the supernatant, concentrates 3 hours in 60 DEG C, spray
Mist is dried, and obtains Fructus Hippophae powder；Being prepared as of described apple peel powder: be dry, pulverize by Pericarpium Mali pumilae, sieves, and obtains apple peel powder；Described flower
Being prepared as of raw clothing powder: take Testa arachidis hypogaeae, add the water of 8 times, decoct 1.5 hours, take the supernatant, be condensed into cream, is dried, obtains flower
Raw clothing powder.
Described finished product of preparing is: weigh Hericium Erinaceus Polysaccharide, Fructus Hippophae powder, Lactobacillus powder preparation, Pericarpium Mali pumilae respectively
Powder, prebiotics, Testa arachidis hypogaeae powder and starch, shared weight portion is respectively (every part is 800g): Hericium Erinaceus Polysaccharide 26 parts, Fructus Hippophae powder 9
Part, 25 parts of Lactobacillus dry powder, apple peel powder 7 parts, (prebiotics used by the present embodiment is soybean oligo saccharide and oligomeric to prebiotics
Xylose equal proportion combines) 3 parts, 6 parts of Testa arachidis hypogaeae powder, starch (starch used by the present embodiment is wheaten starch, corn starch and
Sweet potato starch equal proportion combines) 55 parts, make tablet, every tablet weight 200mg through dry method.
The eating effect of the active lactic acid bacteria preparation that test example 1 contains Hericium Erinaceus Polysaccharide to verify gained of the present invention is (real
Execute example 5), We conducted contrast test.In October ,-2013 in April, 2012, choose 240 gastrointestinal dysfunction persons, wherein disappear
Change bad 60 examples, low 80 examples of appetite, Constipation In 100 Cases.Being randomly divided into two groups (often organizing 120 examples), two groups of eater's each side are entered
Row compares, and there was no significant difference, has comparability.Matched group eats commercially available oral lactobacillus preparation, and experimental group eats this
Bright products obtained therefrom.
Curative effect situation criterion: effective: symptom is clearly better and even disappears；Take a turn for the better: symptom alleviates；Invalid: symptom without
It is obviously improved.Always effectively include effective and take a turn for the better.It is as shown in the table for result.
Table 1: gained of the present invention contains the active lactic acid bacteria preparation compliance test result of Hericium Erinaceus Polysaccharide
As can be seen from Table 1, after edible 15 days, effective 56 people of experimental group, take a turn for the better 40 people, invalid 24 people, total effective rate 80%, food
After 30 days, effective 80 people, take a turn for the better 36 people, invalid 4 people, total effective rate 96.67%；After edible 15 days, effective 12 people of matched group,
Take a turn for the better 30 people, invalid 78 people, total effective rate 35%；After edible 30 days, effective 37 people, take a turn for the better 48 people, invalid 35 people, total effective rate
70.83%.The eating effect of experimental group is substantially better than matched group.
Finally it should be noted that embodiment is the optimum detailed description of the invention of the present invention, be not limited to this
Invention, although being described in detail the present invention with reference to previous embodiment, for a person skilled in the art, it depends on
So the technical scheme described in foregoing embodiments can be modified, or wherein portion of techniques feature is carried out equivalent replace
Change.All within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included in the present invention
Protection domain within.
Claims (10)
1. the preparation method of the active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide, it is characterised in that enter as steps described below
OK: the preparation of (1) Hericium erinaceus (Bull. Ex Fr.) Pers. Polysaccharides；(2) preparation of Lactobacillus powder preparation；(3) Fructus Hippophae powder, apple peel powder and Semen arachidis hypogaeae
The preparation of clothing powder；(4) finished product is prepared.
2. the preparation method of the active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide as described in claim 1, it is characterised in that
The preparation of described Hericium Erinaceus Polysaccharide is divided into following five steps: the pretreatment of (1) Hericium erinaceus (Bull. Ex Fr.) Pers. raw material；(2) Hericium Erinaceus Polysaccharide is molten
Go out；(3) little molecule is removed in ultrafiltration；(4) precipitate with ethanol of Hericium Erinaceus Polysaccharide；(5) vacuum lyophilization；
(1) pretreatment of Hericium erinaceus (Bull. Ex Fr.) Pers. raw material: take and be dried the hericium erinaceus fruiting body to constant weight, pulverizes, is placed in the 65 of 6-13 times of volume
～90% soak 20～28 h in ethanol solution, with remove fatty, part single (few) is sugared and fat-soluble pigment, wind at residue cool place
Dry；
(2) dissolution of Hericium Erinaceus Polysaccharide: after residue air-dries, the selective extraction time is 1.2～1.8 h, and temperature is 70～90 DEG C, water
Material ratio is 24～28mL/g(v/w), 110 mesh filtered through gauze, filtering residue repeats to extract 3 times；
(3) little molecule is removed in ultrafiltration: step (2) gained filtrate merged, and using ultrafilter membrane molecular weight is the hollow cellulose of 10,000
Film carries out ultrafiltration, removes the small-molecule substance of less than 10,000；
(4) precipitate with ethanol of Hericium Erinaceus Polysaccharide: add dehydrated alcohol in the extracting solution after ultrafiltration concentration, until the ethanol terminal of solution is dense
Degree is 65～85%, and 4 DEG C stand 24 h, centrifugal, take sediment fraction, lyophilization；
(5) vacuum lyophilization: step (4) gained is positioned at 20 DEG C freezing 4h, then proceeds at 80 DEG C freezing
12h, then proceeds to vacuum freeze drier and is dried, obtain Hericium Erinaceus Polysaccharide.
3. the preparation method of the active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide as claimed in claim 2, it is characterised in that institute
State the preparation process (1) of Hericium Erinaceus Polysaccharide to be placed in the 70-85% ethanol solution of 8～11 times of volumes immersion 23～26 h(and preferably put
25 are soaked h) in 80% ethanol solution of 9 times of volumes.
4. the preparation method of the active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide as claimed in claim 2, it is characterised in that institute
State the preparation process (4) of Hericium Erinaceus Polysaccharide until the ethanol endpoint concentration of solution be 70～80%(preferably of up to solution ethanol eventually
Point concentration is 75%).
5. the preparation method of the active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide as described in claim 1, it is characterised in that
Being prepared as of described Lactobacillus powder preparation:
(1) dispensing: raw material milk is warming up to 45～55 DEG C, addition 0.15～the low methoxyl pectin of 0.25% and 9～the white sugar of 15%,
Fully mixing；
(2) homogenizing: feed liquid step (1) prepared carries out homogenizing at 15～30 Mpa, under the conditions of 60～75 DEG C；
(3) sterilizing: the feed liquid after homogenizing is continued 8～12min at 95 DEG C, is cooled to 40 DEG C～45 DEG C；
(4) inoculation fermentation: by fermentating liquid volume 4～the amount inoculating compound bacterium agent of 7%, temperature 45 C stands constant temperature culture 5～7 h,
When acidity reaches 70～75 ° of T, product is cooled to cold preservation after-ripening 15～26 h at 2～6 DEG C, obtains curdy cultured milk；
(5) it is dried, makes powder: be dried by step (4) gained curdy cultured milk 50 DEG C of spray dryers of employing, prepare activity
Lactic acid bacteria powder preparation.
6. the preparation method of the active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide as claimed in claim 5, it is characterised in that institute
State composite bacteria agent capable described in the preparation process (4) of Lactobacillus powder preparation by streptococcus thermophilus, Bulgaria's lactic acid bar
Two kinds or three kinds or four kinds in bacterium, lactobacillus casei, bacillus bifidus combine.
7. the preparation method of the active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide as described in claim 1, it is characterised in that
Being prepared as of described Fructus Hippophae powder: take Fructus Hippophae, squeezes extracting juice, filters, takes the supernatant, concentrates 3 hours in 50-60 DEG C, spray dried
Dry, obtain Fructus Hippophae powder；Being prepared as of described apple peel powder: be dry, pulverize by Pericarpium Mali pumilae, sieves, and obtains apple peel powder；Described Testa arachidis hypogaeae
Being prepared as of powder: take Testa arachidis hypogaeae, adds the water of 5-8 times, decocts 1.5 hours, takes the supernatant, be condensed into cream, is dried, obtains Semen arachidis hypogaeae
Clothing powder.
8. the preparation method of the active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide as described in claim 1, it is characterised in that
Described finished product of preparing is: weigh Hericium Erinaceus Polysaccharide, Fructus Hippophae powder, Lactobacillus powder preparation, apple peel powder respectively, prebiotic
Unit, Testa arachidis hypogaeae powder and starch, shared weight portion is respectively as follows: Hericium Erinaceus Polysaccharide 18-26, Fructus Hippophae powder 5-9, Lactobacillus dry powder
20-25, apple peel powder 3-7, prebiotics 1.5-3, Testa arachidis hypogaeae powder 2-6, starch 43-55, make tablet, every tablet weight through dry method
200mg。
9. the preparation method of the active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide as claimed in claim 8, it is characterised in that institute
Stating prebiotics is the one in soybean oligo saccharide and oligomeric xylose or two kinds of combinations.
10. the preparation method of the active lactic acid bacteria preparation containing Hericium Erinaceus Polysaccharide as claimed in claim 8, it is characterised in that
Described starch is wheaten starch, corn starch, the one in sweet potato starch, the combination of two or three.
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| CN114806920A (en) * | 2022-02-28 | 2022-07-29 | 广州栋方生物科技股份有限公司 | Culture medium of bifidobacterium as well as culture method and application thereof |
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