CN108752092B - Preparation method of pesticide degrading bacteria organic fertilizer prepared from biogas residues in pig farm - Google Patents
Preparation method of pesticide degrading bacteria organic fertilizer prepared from biogas residues in pig farm Download PDFInfo
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- CN108752092B CN108752092B CN201810566335.8A CN201810566335A CN108752092B CN 108752092 B CN108752092 B CN 108752092B CN 201810566335 A CN201810566335 A CN 201810566335A CN 108752092 B CN108752092 B CN 108752092B
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- fertilizer
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
- C05G5/37—Layered or coated, e.g. dust-preventing coatings layered or coated with a polymer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a preparation method of a pesticide degrading bacteria organic fertilizer prepared from biogas residues in a pig farm, and belongs to the technical field of biological organic fertilizers. The organic fertilizer of the pesticide degrading bacteria is prepared by taking zeolite as an organic fertilizer carrier, biogas residue as a substrate, HD-1 bacterial liquid as an additive and polyvinyl alcohol as a coating material. The polyvinyl alcohol adopted by the invention has stable property at normal temperature, is non-toxic and harmless, has a certain hierarchical structure, can be filled in the internal gaps of the fertilizer, and structurally improves the stability and fertilizer retention of the fertilizer. The zeolite can reduce the fixation of the soil to the quick-acting phosphorus, has a certain exchange adsorption effect on ammonium nitrogen, and ammonium ions dissolved in the biogas residues can be adsorbed and retained by the zeolite, so that the release rate of nutrient elements in the fertilizer is controlled, and the slow release performance is improved. The pesticide degrading bacteria organic fertilizer can improve soil, enhance soil scab resistance and compaction resistance, improve activity of soil enzymes, and degrade residual pesticide substances in soil through microorganisms.
Description
Technical Field
The invention belongs to the technical field of biological organic fertilizers, and particularly relates to a method for preparing a biological bacterial organic fertilizer by combining biogas residues in a pig farm and pesticide degrading bacteria HD-1.
Background
The farmland farming system in southern areas of China is generally ripe twice a year, and long-term unreasonable use of chemical fertilizers can reduce soil quality, change the granular structure of soil, make soil hardening and salinization degrees worse, inhibit the activity of microorganisms in the soil and cause serious damage to the ecological environment of the soil. The research of the agrochemicals shows that the cost of applying the fertilizer is increased year by year, but the benefit is continuously reduced. Meanwhile, a large amount of pesticide remains in soil in China, and if the pesticide is not repaired, the soil environment and the human health are greatly damaged. The application of the organic fertilizer can increase the content of nitrogen and phosphorus in the soil, improve the aggregation and ventilation structure of the soil, enhance the scab resistance and compaction resistance of the soil and improve the activity of soil enzymes and microorganisms. On the other hand, the organic fertilizer can promote the growth of crops and improve the quality and the yield of the crops.
Disclosure of Invention
The main purposes of the invention are:
aiming at the problems of large biogas residue yield, low utilization efficiency, serious pesticide pollution to farmlands and the like in pig farms in China. The invention provides a green and environment-friendly organic fertilizer preparation method, which takes zeolite as an organic fertilizer carrier, biogas residue as a substrate, HD-1 bacterial liquid as an additive and polyvinyl alcohol as a coating material to prepare the organic fertilizer of pesticide degrading bacteria.
The main technical scheme of the invention is as follows:
a preparation method of a pesticide degrading bacteria organic fertilizer prepared from biogas residues in a pig farm comprises the following steps:
(1) preparing a bacterial liquid: preparing fresh overnight HD-1 bacterial liquid;
(2) preparing a coating material: heating a certain amount of distilled water to about 90 ℃, adding polyvinyl alcohol, stirring continuously, keeping the temperature, stirring until the polyvinyl alcohol is completely dissolved, preparing a polyvinyl alcohol solution with the mass fraction of 10%, sealing, storing and cooling to room temperature;
(3) drying biogas residues: drying the biogas residues in the pig farm for 72h at the high temperature of 150 ℃ by using a dryer, and removing impurities;
(4) crushing: respectively crushing the zeolite and the biogas residues into 60-mesh powder by using a crusher;
(5) mixing materials: uniformly mixing 20-30 parts of zeolite and 65-75 parts of biogas residue, and adding a certain amount of HD-1 bacterial liquid in the uniformly mixing process;
(6) and (3) granulation: granulating with a disk granulator, adding prepared polyvinyl alcohol solution with mass of 8%, and mixing at a ratio of 0.2 mL/g-1The particle size is controlled to be 3-5 mm, so that the effective viable count is more than 0.2cfu g-1。
(7) Drying and forming: and drying the wet fertilizer by drying to obtain the finished product of the pesticide degrading bacteria organic fertilizer.
The above strain HD-1, Pseudomonas mosselii, now deposited in the general microbiological center of the China Committee for culture Collection of microorganisms, address: the institute of microbiology, national academy of sciences No. 3, Xilu No.1, Beijing, Chaoyang, Beijing. The preservation number is: CGMCC NO.15123, preservation time: 26 months 12 in 2017; the proposed classification is the Pseudomonas mosselii genus Pseudomonas morganii.
The main principle of the invention is as follows: (1) the biogas residues contain a large amount of organic matters, and the organic matters can increase the content of nitrogen and phosphorus in soil, improve the aggregation and ventilation structure of the soil, enhance the scab resistance and compaction resistance of the soil and improve the activity of soil enzymes and microorganisms. (2) The zeolite powder has small density and strong adsorption capacity and caking property, contains a large amount of silicate crystalline minerals, can help polyvinyl alcohol to better cover the surface of the granular fertilizer, forms a solid barrier to wrap the fertilizer in the zeolite powder, effectively prevents water molecules from immersing and inhibits the release of nutrient ions, and can reduce the fixation of soil to available phosphorus, thereby improving the slow release effect of the fertilizer, and preventing the nutrient from being released too fast to generate the seedling burning phenomenon. (3) After the pesticide degrading bacteria organic fertilizer is applied to soil, water molecules start to contact the surface of the fertilizer, pores are gradually generated on the surface of the film, the water molecules enter the film and start to be immersed into the granular fertilizer to dissolve nutrients in the fertilizer to form a saturated solution, and a matrix in the fertilizer is continuously expanded, so that a water vapor pressure difference or a osmotic pressure difference is generated, the internal tension is continuously increased, and finally HD-1 degrading bacteria are diffused outwards through the micropores in the film to enter the soil and degrade pesticides in the soil through the actions of dissolution, enzymolysis, cell phagocytosis and the like of microorganisms.
The main advantages of the invention are as follows:
(1) the adopted polyvinyl alcohol has stable property, is non-toxic and harmless at normal temperature, has a certain hierarchical structure, can be filled in the internal gaps of the fertilizer, and structurally improves the stability and fertilizer retention of the fertilizer.
(2) The zeolite can reduce the fixation of the soil to the quick-acting phosphorus, has a certain exchange adsorption effect on ammonium nitrogen, and ammonium ions dissolved in the biogas residues can be adsorbed and retained by the zeolite, so that the release rate of nutrient elements in the fertilizer is controlled, and the slow release performance is improved.
(3) The nutrient dissolution rates of the pesticide degrading bacteria organic fertilizer prepared from the biogas residues in the pig farm in total phosphorus and total nitrogen are lower than 15 percent and less than 1 percent respectively at the initial stage, and the cumulative nutrient dissolution rate within 28d is lower than 80 percent, thereby meeting the requirements of Chinese slow release fertilizers (GB/T23348) -2009) and being used as cross-season long-term fertilizers.
(4) The pesticide degrading bacteria organic fertilizer prepared by the invention can improve soil, enhance the scab resistance and compaction resistance of the soil, improve the activity of soil enzymes, and degrade residual pesticide substances in the soil through microorganisms.
Detailed Description
The following 4 examples are provided to further illustrate the invention. The examples are intended to illustrate the invention, but not to limit it.
The strain HD-1 used in the invention is a Pseudomonas morganii (Pseudomonas mosselii) screened from soil by the inventor, and is deposited in the China general microbiological culture Collection center, with the address: the institute of microbiology, national academy of sciences No. 3, Xilu No.1, Beijing, Chaoyang, Beijing. The preservation number is: CGMCC NO.15123, preservation time: 26 months 12 in 2017; the proposed classification is the Pseudomonas mosselii genus Pseudomonas morganii.
Example 1
The preparation steps are as follows:
1. preparing bacterial liquid: fresh and overnight HD-1 bacterial liquid was prepared.
2. Preparing a coating material: heating a certain amount of distilled water to about 90 ℃, adding polyvinyl alcohol, stirring continuously, keeping the temperature, stirring until the polyvinyl alcohol is completely dissolved, preparing a polyvinyl alcohol solution with the mass fraction of 8%, sealing, storing and cooling to room temperature.
3. Pretreating biogas residues: drying the biogas residue with the water content of 60% at 150 ℃ for 72 h.
4. Granulation pretreatment: the zeolite and the biogas residue are respectively crushed into 60-mesh powder by a crusher.
5. Compounding and mixing: and (3) uniformly mixing 20 parts of zeolite and 75 parts of biogas residue, and adding a certain amount of HD-1 bacterial liquid in the uniformly mixing process.
6. The mixed materials are put into a disc granulator for granulation, and 8 percent of polyvinyl alcohol is added, and the mixing ratio is 0.2 mL-g-1The particle size range is 3-5 mm, so that the effective viable count is more than 0.2cfu g-1。
7. Drying and forming: and drying the granular wet fertilizer, and screening and packaging the dried granular organic fertilizer.
Example 2
The preparation was as in example 1, except that:
1. preparing bacterial liquid: fresh and overnight HD-1 bacterial liquid was prepared.
2. Preparing a coating material: heating a certain amount of distilled water to about 90 ℃, adding polyvinyl alcohol, stirring continuously, keeping the temperature, stirring until the polyvinyl alcohol is completely dissolved, preparing a polyvinyl alcohol solution with the mass fraction of 10%, sealing, storing and cooling to room temperature.
3. Pretreating biogas residues: drying the biogas residue with the water content of 65% at the high temperature of 150 ℃ for 72 h.
4. Granulation pretreatment: the zeolite and the biogas residue are respectively crushed into 60-mesh powder by a crusher.
5. Compounding and mixing: and (3) uniformly mixing 25 parts of zeolite and 70 parts of biogas residue, and adding a certain amount of HD-1 bacterial liquid in the uniformly mixing process.
6. The mixed materials are put into a disc granulator for granulation, 10 percent of polyvinyl alcohol is added, and the mixing ratio is 0.2 mL-g-1The particle size range is 3-5 mm, so that effective viable bacteria can be obtainedThe number is more than 0.2cfu g-1。
7. Drying and forming: and drying the granular wet fertilizer, and screening and packaging the dried granular organic fertilizer.
Example 3
The preparation was as in examples 1 and 2, except that:
1. preparing bacterial liquid: fresh and overnight HD-1 bacterial liquid was prepared.
2. Preparing a coating material: heating a certain amount of distilled water to about 90 ℃, adding polyvinyl alcohol, stirring continuously, keeping the temperature, stirring until the polyvinyl alcohol is completely dissolved, preparing a polyvinyl alcohol solution with the mass fraction of 15%, sealing, storing and cooling to room temperature.
3. Pretreating biogas residues: drying the biogas residue with the water content of 65% at the high temperature of 150 ℃ for 72 h.
4. Granulation pretreatment: the zeolite and the biogas residue are respectively crushed into 60-mesh powder by a crusher.
5. Compounding and mixing: and (3) uniformly mixing 30 parts of zeolite and 65 parts of biogas residues, and adding a certain amount of HD-1 bacterial liquid in the uniformly mixing process.
6. The mixed materials are put into a disc granulator for granulation, 15 percent of polyvinyl alcohol is added, and the mixing ratio is 0.2 mL-g-1The particle size range is 3-5 mm, so that the effective viable count is more than 0.2cfu g-1。
7. Drying and forming: and drying the granular wet fertilizer, and screening and packaging the dried granular organic fertilizer.
The test method comprises the steps of taking two parts of 400g of soil with the concentration of 2,4-DCP being 30mg/kg, adding 267mL of HD-1 bacterial liquid and 10g of bacterial manure into one part of the soil, keeping the water content at 40% while keeping the other part blank, taking 10g of soil into a 50mL centrifuge tube every two days, adding 40mL of distilled water, carrying out ultrasonic extraction for 30min, centrifuging for 20min under the condition of 4000r/min, taking supernate to pass through a 0.45 mu m pinhole filter to obtain leaching liquor samples in different time periods, detecting the leaching samples by a luminous bacterial toxicity detection analyzer (JQ LID-P1), and expressing the determination result by light inhibition rate. The effect of degrading the toxicity of the soil pesticide is shown in table 1, and the toxicity of the soil pesticide can be effectively reduced.
TABLE 1
Claims (2)
1. A preparation method of a pesticide degrading bacteria organic fertilizer prepared from biogas residues in a pig farm is characterized by comprising the following steps:
(1) preparing a bacterial liquid: preparing fresh overnight HD-1 bacterial liquid; strain HD-1, accession number: CGMCC No.15123, suggested classification name is Pseudomonas mosselii;
(2) preparing a coating material: heating distilled water to 90 ℃, adding polyvinyl alcohol, stirring continuously, keeping the temperature, stirring until the polyvinyl alcohol is completely dissolved, preparing a polyvinyl alcohol solution with the mass fraction of 8%, sealing, storing and cooling to room temperature;
(3) drying biogas residues: drying the biogas residues in the pig farm for 72h at the high temperature of 150 ℃ by using a dryer, and removing impurities;
(4) crushing: respectively crushing the zeolite and the biogas residues into 60-mesh powder by using a crusher;
(5) mixing materials: uniformly mixing zeolite and biogas residue, and adding HD-1 bacteria liquid in the uniformly mixing process;
(6) and (3) granulation: granulating with a disk granulator, adding prepared polyvinyl alcohol solution with mass of 8%, and mixing at a ratio of 0.2 mL/g-1The particle size is controlled to be 3-5 mm, so that the effective viable count is more than 0.2cfu g-1；
(7) Drying and forming: and drying the wet fertilizer by using a dryer to obtain the finished product of the pesticide degrading bacteria organic fertilizer.
2. The preparation method of the organic fertilizer containing the pesticide degrading bacteria from the biogas residues in the pig farm according to claim 1, wherein 20-30 parts of zeolite and 65-75 parts of biogas residues are uniformly mixed.
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| RU2484131C2 (en) * | 2011-07-13 | 2013-06-10 | Общество с ограниченной ответственностью "БИОЛЭНД" | Biopreparation for treatment of water, industrial drains and soil from pesticides resistant to decomposition and method of its application |
| KR20150076615A (en) * | 2013-12-27 | 2015-07-07 | 경북대학교 산학협력단 | Pseudomonas mosselii SJ10 having ε-caprolactam degrading activity and method for degrading ε-caprolactam using the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101333053A (en) * | 2008-08-05 | 2008-12-31 | 中国石化扬子石油化工有限公司 | Processing method for waste water in production process of fine terephthalic acid |
| RU2484131C2 (en) * | 2011-07-13 | 2013-06-10 | Общество с ограниченной ответственностью "БИОЛЭНД" | Biopreparation for treatment of water, industrial drains and soil from pesticides resistant to decomposition and method of its application |
| CN103748214A (en) * | 2011-07-13 | 2014-04-23 | 生物地带有限公司 | Biological product for clearing of water, industrial wastewater and soil from chemicals resistant to degradation, and method for using the same |
| KR20150076615A (en) * | 2013-12-27 | 2015-07-07 | 경북대학교 산학협력단 | Pseudomonas mosselii SJ10 having ε-caprolactam degrading activity and method for degrading ε-caprolactam using the same |
Non-Patent Citations (3)
| Title |
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| "分散式养猪场粪污/秸秆混合发酵沼渣制备缓释肥及缓释性能研究";黄彬等;《天津农业科学》;20180413;第24卷(第4期);第39-44页 * |
| "降解2, 4-二氯酚微生物的分离及其2, 4-二氯酚羟化酶基因的克隆和表达";钟文辉等;《生物工程学报》;20040323;第20卷(第2期);第209-214页 * |
| Biodegradation effects of o-cresol by Pseudomonas monteilii SHY on mustard seed germination.;Krishnan Shainy Nhattuketty等;《Bioinformation》;20180526;第14卷(第6期);第271-278页 * |
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