CN108740353A - A kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed - Google Patents
A kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed Download PDFInfo
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Abstract
The present invention relates to the preparation methods that a kind of livestock and poultry are acidified calcium salt glycolysis feed, belong to feed processing technology field.The preparation method comprises the following steps：Starch material is sized mixing after enzymatic saccharification, lactic acid producing strain fermentation is accessed, thalline acid sludge wet basis and the lactic acid concentrated calcium solution containing lactic acid is made after separation concentration in stream plus calcium hydroxide fermentation；Starch material is sized mixing after enzymatic saccharification, citric acid acid sludge wet basis and citric acid concentrate is made after separation concentration in access production citric acid strain fermentation；Glycolysis slag dregs of rice wet basis is made in glycolysis after one or more of sugar residue wet basis, thalline acid sludge wet basis, citric acid acid sludge wet basis are mixed with feedstuff, clean water, compound bacteria, complex enzyme；Lactic acid concentrated calcium solution, citric acid concentrate and glycolysis slag dregs of rice wet basis are mixed to prepare livestock and poultry and are acidified calcium salt glycolysis feed.Feed prepared by the present invention has the characteristics that calcium absorptivity height, anti diar rhea, palatability are good rich in nutritional ingredients such as citric acid, calcium lactate, lactic acid, small peptide, oligosaccharide.
Description
Technical field
The invention belongs to the preparation sides that feed processing technology field more particularly to a kind of livestock and poultry are acidified calcium salt glycolysis feed
Method.
Background technology
China is aquaculture big country.2015, the animal husbandry output value was up to 3.2 trillion yuans, meat, birds, beasts and eggs, milk and aquatic products
Yield respectively reaches 84,540,000 tons, 28,000,000 tons, 38,000,000 tons and fifty-five million ton.Along with the development of aquaculture, feed industry
Development speed is surprising.According to Ministry of Agriculture's statistical data, national commercial feed yield reaches 2.18 hundred million tons within 2015, wherein full price
1.79 hundred million tons of feed, 21,510,000 tons of concentrated feed, 6,400,000 tons of premix, including 89,740,000 tons of pig feed yield, fowl material yield 9000
Ten thousand tons, 53,000,000 tons of aquatic feeds, 8,500,000 tons of cattle and sheep feed, 3,500,000 tons of the feeds such as rabbit racoon dog pet.
It is adding ingredient important in feed it is well known that calcium constituent as a kind of trace element, has multiple functions.
Domestic feeding calcium additive is generally divided into inorganic calcium and organic calcium.Wherein, inorganic calcium is based on mountain flour and calcium carbonate.Mountain flour and carbon
Although sour calcium calcium content is high, solubility is relatively low, big to GI irritation, and hydrochloric acid in gastric juice is needed to dissolve, the insufficient suckling piglet of hydrochloric acid in gastric juice
It is more difficult to absorb.Meanwhile mountain flour and calcium carbonate system acid power are stronger, be easy to cause suckling piglet diarrhea.The general dissolubility of calcium of organic acid
It is good, it is small to GI irritation, it is sour power very little.Calcium of organic acid mainly has calcium lactate, calcium citrate etc..The calcium content of calcium of organic acid
Although more slightly lower than inorganic calcium, many researchs both at home and abroad all show that the absorptivity of calcium of organic acid and bioavailability are apparently higher than
Inorganic calcium, especially calcium lactate.Calcium lactate easily absorbs, and not stimulating intestine and stomach, is not easy to cause constipation, is more suitable for the feed for piglet.
Currently, domestic food grade calcium lactate is the refined place such as neutralized zymotic fluid, acidolysis, decoloration, ion-exchange and crystallization mostly
The product of food grade lactic acid and calcium carbonate reaction after reason.It is that organic acid is isolated and purified out to neutralize with acidolysis, and the two can
A large amount of waste water and waste liquid is generated, pollution is quite serious, and consume energy water consumption, of high cost.Decoloration is to slough the substances such as vitamin and pigment
Or nutrition, ion-exchange are to slough mineral element, such as calcium, phosphorus, magnesium, sodium, potassium, chlorine, sulphur, iron, copper, zinc, manganese, iodine, cobalt, selenium.Decoloration
A large amount of waste water, waste liquid often can be all generated with ion-exchange, pollution weight is of high cost.Therefore, feeding not neutralize, not acidolysis, do not take off
Color, non-ion-exchange original flavor product by be future development direction.However, the work for not yet finding lactic acid nondiscoloration at present and directly extracting
Skill, do not find not neutralize yet, not acidolysis, nondiscoloration, non-ion-exchange organic acid fermentation liquid directly react and the feed grade breast that produces
Sour calcium product.
Invention content
In view of this, the purpose of the present invention is to provide a kind of livestock and poultry simple for process, at low cost, free of contamination to be acidified calcium salt
The preparation method of glycolysis feed.
The present invention provides the preparation methods that a kind of livestock and poultry are acidified calcium salt glycolysis feed, include the following steps：
Calcium source is mixed with citric acid concentrate, obtain livestock and poultry acidification calcium salt glycolysis feed, the calcium source be calcium lactate and/
Or calcium citrate；
The preparation method of the citric acid concentrate includes：
Starch-containing product is crushed or sugar-containing product is beaten, obtains enzymolysis raw material；The enzymolysis raw material is mixed into tune with water
Slurry, adds amylase enzymolysis liquefying-saccharifying, and band slag saccharified liquid is made；
After 29~31 DEG C of 62~70h of fermentation of production citric acid strain being accessed to the band slag saccharified liquid, centrifugation or filtering fermentation
Liquid, collection liquid phase component are citric acid thin liquid, and solid phase components are citric acid acid sludge wet basis, are concentrated by evaporation the citric acid thin liquid,
Obtain citric acid concentrate；
The preparation method of the calcium citrate includes：
Stream plus calcium hydroxide or carbon in above-mentioned citric acid concentration liquid and preparation method thereof access production citric acid strain fermentation process
Sour calcium, centrifugation or filtering fermentating liquid, collection solid phase components are calcium citrate acid sludge wet basis, are contained in the calcium citrate acid sludge wet basis
There is calcium citrate；
Or by the citric acid thin liquid and calcium hydroxide or calcium carbonate in not exclusively and under conditions of mix, collect and consolidate
Phase component is calcium citrate；
The preparation method of the calcium lactate includes：
Starch-containing product is crushed or sugar-containing product is beaten, obtains enzymolysis raw material；The enzymolysis raw material is mixed into tune with water
Slurry, adds amylase enzymolysis, liquefaction, saccharification, and band slag saccharified liquid is made；
To after 48~50 DEG C of 24~58h of fermentation of band slag saccharified liquid access lactic acid producing strain, centrifugation or filtering fermentating liquid,
Collection liquid phase component is calcium lactate；Solid phase components are sugar residue thalline acid sludge wet basis；
Or be separated by solid-liquid separation the band slag saccharified liquid, it is no slag saccharified liquid to collect liquid phase component, and solid phase components are sugar residue
Wet basis, to after 24~58h of the no slag saccharified liquid access lactic acid producing strain fermentation, centrifugation or filtering fermentating liquid collect liquid phase group
It is divided into calcium lactate, solid phase components are thalline acid sludge wet basis；
Or by molasses ion-exchange deferrization, adds water to size mixing obtained liquid molasses, lactic acid producing strain hair is accessed into the liquid molasses
After 24~58h of ferment, centrifugation or filtering fermentating liquid, collection liquid phase component are calcium lactate.
Livestock and poultry acidification calcium salt glycolysis feed also contains glycolysis slag dregs of rice wet basis, and the glycolysis slag dregs of rice wet basis is by by glycolysis
It is obtained after raw material enzymatic hydrolysis and fermentation, the glycolysis raw material includes feedstuff, clean water, strain liquid and complex enzyme；The enzymolysis hair
Ferment temperature is 34~37 DEG C, and enzymatic hydrolysis and fermentation initial ph value is 6.8,81~91h of total time of the enzymatic hydrolysis and fermentation.
Preferably, the glycolysis raw material further includes sugar residue wet basis, thalline acid sludge wet basis, sugar residue thalline acid sludge wet basis, lemon
One or more of acid sludge wet basis and calcium citrate acid sludge wet basis.
Preferably, the glycolysis raw material replaces with clean water, strain liquid and complex enzyme and sugar residue wet basis, thalline acid sludge are wet
The compound acquisition of one or more of base, sugar residue thalline acid sludge wet basis, citric acid acid sludge wet basis and calcium citrate acid sludge wet basis.
Preferably, livestock and poultry acidification calcium salt glycolysis feed also contain feeding organic acid, feeding inorganic acid, acylate,
One or more of sugar molasse syrup and liquid fat.
Preferably, the feedstuff is the one or more of feedstuff in Minister Agriculture of China's feedstuff catalogue, packet
Include dregs of beans, cotton dregs, rapeseed dregs, peanut meal, fish meal, blood meal, plasma proteins, hyperglobulinemia, milk powder, lactose, whey powder, wheat
Gluten, rice protein powder, DDGS, soybean, pea, red bean, corn, paddy, is cracked rice, wheat, wheat bran, rice at corn protein powder
Chaff, flour, oat, barley, sweet potato, soybean protein isolate, soy protein concentrate, pasture powder, Chinese herbal medicine, fruit and vegetable and carbohydrate
It is one or more in the dilated product of molasses syrup or above-mentioned feedstuff；
The strain liquid includes lactobacillus inoculation liquid, barms liquid, bacillus subtilis strain liquid, bacillus licheniformis kind
It is one or more in liquid, bifidobacterium species atcc liquid, aspergillus oryzae kind liquid, Brevibacterium acetylicum kind liquid and clostridium tyrobutyricum kind liquid；
The complex enzyme includes protease, zytase, dextranase, mannonase pectase, α-amylase, saccharification
It is a variety of in enzyme, carbohydrase and cellulase；
The feeding organic acid or its concentrate include for fumaric acid, malic acid, a-ketoglutaric acid, succinic acid, tartaric acid,
Acetic acid, propionic acid, butyric acid, ascorbic acid, salicylic acid, gibberellic acid, kojic acid, gluconic acid, methylene succinic acid, formic acid, benzoic acid, wine
It is one or more in stone acid and fumaric acid and sorbic acid；
The feeding inorganic acid includes one or more in phosphoric acid, hydrochloric acid and sulfuric acid；
The acylate includes calcium formate, sodium propionate, calcium propionate, sodium butyrate, sodium benzoate, sodium sorbate, sorbic acid
It is one or more in potassium, potassium citrate, sodium citrate and calcium citrate；
The sugar molasse syrup include sucrose, glucose, fructose, glucose syrup, maltodextrin slurry/powder, fructose syrup/
It is one or more in powder, malt syrup/powder, Sugar cane molasses and beet molasses；
The liquid fat includes soybean oil and its oil emulsion, corn oil and its oil emulsion, palm oil and its oil emulsion, rice
Oil extracted from rice husks and its oil emulsion, coconut oil and its oil emulsion, fish oil and animal oil and its oil emulsion, peanut oil and its oil emulsion, vegetable seed
It is one or more in oil and its oil emulsion, tea oil and its oil emulsion, plants essential oil.
Preferably, the fermentation pH of the production citric acid fungus strain is nature pH；Fermented type is aerobic fermentation；Fermentation time
For 63~69h；
The fermentation pH value of the lactic acid producing strain fermentation is 3~9；Fermented type is anaerobic fermentation；Fermentation time be 50~
56h。
Preferably, the sugar residue wet basis, thalline acid sludge wet basis/sugar residue thalline acid sludge wet basis, citric acid acid sludge wet basis and feeding
Expect raw material, clean water, strain liquid, complex enzyme weight ratio be 100：0~70：0~38：220~789：0~186：12~19：
0.008~1.9.
Preferably, the lactic acid bacteria culturers liquid in the strain liquid：Yeast seeds liquid：The work of Bacillus licheniformis strain liquid
Bacterium number amount ratio is 100：11~28：6~9；
Protease in the complex enzyme：Zytase：Dextranase：Mannase：The weight ratio of pectase is
100：62~78：61~79：63~78：31~45.
Preferably, the calcium lactate, citric acid concentrate and glycolysis slag dregs of rice wet basis and feeding organic acid, feeding inorganic acid,
Acylate, sugar molasse syrup, liquid fat weight ratio be 100：213~798：51~199：23~38：11~19：11
~19：51~95：11~19.
Beneficial effects of the present invention：Livestock and poultry prepared by the present invention are acidified calcium salt glycolysis feed, rich in citric acid, calcium lactate,
The battalion such as lactic acid, small peptide, oligosaccharide, probiotics, other organic acids, protein, crude fibre, crude fat, vitamin and mineral element
It forms point, has that good calcium absorptivity height, anti diar rhea, palatability, amino acid balance, nutrition be high, easy to digest and anti-nutritional factors contains
Measure the features such as low.
Specific implementation mode
The present invention provides the preparation methods that a kind of livestock and poultry are acidified calcium salt glycolysis feed, include the following steps：By calcium source with
Citric acid concentrate mixes, and obtains livestock and poultry acidification calcium salt glycolysis feed.
In the present invention, the calcium source is calcium lactate and/or calcium citrate；In the present invention, the citric acid concentrate
Preparation method include：Starch-containing product is crushed or sugar-containing product is beaten, obtains enzymolysis raw material；By the enzymolysis raw material and water
Mixing is sized mixing, and amylase enzymolysis liquefying-saccharifying is added, and band slag saccharified liquid is made；Production citric acid strain is accessed to the band slag saccharified liquid
After 29~31 DEG C of 62~70h of fermentation, centrifugation or filtering fermentating liquid, collection liquid phase component are citric acid thin liquid, and solid phase components are lemon
Lemon acid acid sludge wet basis, is concentrated by evaporation the citric acid thin liquid, obtains citric acid concentrate.The present invention to the starch-containing product or
The specific type of sugar-containing product is not particularly limited, as long as the product is starch-containing or sugar.Heretofore described crushing
Using this field conventional grinder, the granularity of the crushing is preferably 200~1000 mesh.Heretofore described mashing is excellent
Choosing is carried out using beater, and the present invention is not particularly limited the conditional parameter of the mashing, can obtain slurries.
The enzymolysis raw material is mixed with water and is sized mixing after obtaining enzymolysis raw material by the present invention, adds amylase enzymolysis liquefaction sugar
Change, band slag saccharified liquid is made.The weight ratio of the enzymolysis raw material and water is preferably 1 in the present invention：(1~8), more preferably
It is 1：(2~7).The mass ratio of the amylase and enzymolysis raw material is preferably (3.0~20) in the present invention:1500.At this
The saccharification of enzymatic liquefaction described in invention can be without other particular/special requirements using the parameter of this field routine.In the present invention, to institute
After stating 29~31 DEG C of 62~70h of fermentation of band slag saccharified liquid access production citric acid strain, centrifugation or filtering fermentating liquid collect liquid phase group
It is divided into citric acid thin liquid.The fermentation pH of the production citric acid fungus strain is nature pH；Fermented type is aerobic fermentation；Fermentation time
Preferably 63~69h, more preferably 66h.In the present invention it is described centrifugation or filtering using this field routine centrifugation or
Filters, which are realized, to be separated by solid-liquid separation.The present invention is concentrated by evaporation the citric acid thin liquid after obtaining the citric acid thin liquid,
Obtain citric acid concentrate.The evaporation and concentration is preferably concentrated using rotary evaporation in the present invention, the multiple of the concentration
Preferably 5~20 times.
In the present invention, the preparation method of the calcium citrate includes：In the concentration liquid and preparation method thereof access production of above-mentioned citric acid
Stream plus calcium hydroxide or calcium carbonate in citric acid strain fermentation process, centrifugation or filtering fermentating liquid, collection solid phase components are lemon
Sour calcium acid sludge wet basis contains calcium citrate in the calcium citrate acid sludge wet basis.In the present invention, it is described stream plus calcium hydroxide or
After calcium carbonate, production citric acid strain not exclusively in and under conditions of ferment.
In the present invention, the preparation method of the calcium citrate can also be by the citric acid thin liquid and calcium hydroxide or carbon
Sour calcium not exclusively in and under conditions of mix, collections solid phase components be calcium citrate；
In the present invention, the preparation method of the calcium lactate includes：Starch-containing product is crushed or sugar-containing product is beaten, is obtained
Digest raw material；The enzymolysis raw material is mixed with water and is sized mixing, amylase enzymolysis, liquefaction, saccharification are added, band slag saccharified liquid is made；This
Preparation method with slag saccharified liquid described in invention and the preparation side with slag saccharified liquid in above-mentioned citric acid concentrate preparation process
Method is consistent, and details are not described herein.The present invention accesses lactic acid-producing bacteria after obtaining the band slag saccharified liquid, to the band slag saccharified liquid
After 48~50 DEG C of 24~58h of fermentation of kind, centrifugation or filtering fermentating liquid, collection liquid phase component are calcium lactate；Solid phase components are sugar residue
Thalline acid sludge wet basis.The fermentation pH value of the lactic acid producing strain is preferably 3~9 in the present invention, and more preferably 5~7；It is described
The fermented type of lactic acid producing strain is anaerobic fermentation；Fermentation time is preferably 50~56h, more preferably 53h.
In the present invention, the preparation method of the calcium lactate can also be：The band slag saccharified liquid is separated by solid-liquid separation, collection liquid
Phase component is no slag saccharified liquid, and solid phase components are sugar residue wet basis, to the no slag saccharified liquid access lactic acid producing strain fermentation 24~
After 58h, centrifugation or filtering fermentating liquid, collection liquid phase component are calcium lactate, and solid phase components are thalline acid sludge wet basis.
In the present invention, the preparation method of the calcium lactate can also be：By molasses ion-exchange deferrization, water is added to size mixing obtained molasses
Liquid, after accessing 24~58h of lactic acid producing strain fermentation into the liquid molasses, centrifugation or filtering fermentating liquid, collecting liquid phase component is
Calcium lactate.
In the present invention, the livestock and poultry acidification calcium salt glycolysis feed also contains glycolysis slag dregs of rice wet basis, and the glycolysis slag dregs of rice are wet
For base by will be obtained after glycolysis raw material enzymatic hydrolysis and fermentation, the glycolysis raw material includes feedstuff, clean water, strain liquid and compound
Enzyme.The enzymatic hydrolysis and fermentation temperature is preferably 34~37 DEG C, more preferably 35~36 DEG C in the present invention；The enzymatic hydrolysis and fermentation
Initial ph value is preferably 6.8, and the enzymatic hydrolysis and fermentation is preferably 81~91h, more preferably 83h total time.
The feedstuff is the one or more of feedstuff in Minister Agriculture of China's feedstuff catalogue in the present invention,
Including dregs of beans, cotton dregs, rapeseed dregs, peanut meal, fish meal, blood meal, plasma proteins, hyperglobulinemia, milk powder, lactose, whey powder, small
Wheat Gluten, corn protein powder, rice protein powder, DDGS, soybean, pea, red bean, corn, paddy, crack rice, wheat, wheat bran,
Rice bran, flour, oat, barley, sweet potato, soybean protein isolate, soy protein concentrate, pasture powder, Chinese herbal medicine, fruit and vegetable and sugar
It is one or more in the dilated product of class molasses syrup or above-mentioned feedstuff.
Heretofore described strain liquid includes lactobacillus inoculation liquid, barms liquid, bacillus subtilis strain liquid, lichens gemma
One kind or more in bacillus specie liquid, bifidobacterium species atcc liquid, aspergillus oryzae kind liquid, Brevibacterium acetylicum kind liquid and clostridium tyrobutyricum kind liquid
Kind.Lactic acid bacteria culturers liquid in heretofore described strain liquid：Yeast seeds liquid：The viable count of Bacillus licheniformis strain liquid
Amount is than preferably 100：11~28：6~9；More preferably 100:12~27:7~8.
Heretofore described complex enzyme includes protease, zytase, dextranase, mannonase pectase, liquefaction
It is a variety of in enzyme, carbohydrase, carbohydrase and cellulase.Protease in the complex enzyme：Zytase：Dextranase：It is sweet
Reveal dextranase：The weight ratio of pectase is preferably 100：62~78：61~79：63~78：31~45, more preferably 100:
64~77:65~73:66~72:33~44.
In the present invention, the glycolysis raw material further include sugar residue wet basis, thalline acid sludge wet basis, sugar residue thalline acid sludge wet basis,
One or more of citric acid acid sludge wet basis and calcium citrate acid sludge wet basis.In the present invention, the sugar residue wet basis, thalline acid
The weight ratio of slag wet basis/sugar residue thalline acid sludge wet basis, citric acid acid sludge wet basis and feedstuff, clean water, strain liquid, complex enzyme
Preferably 100：0~70：0~38：220~789：0~186：12~19：0.008~1.9, more preferably 100：3~
65：1~37：244~779：3~177：14~18：0.010~1.8.
In the present invention, the glycolysis raw material also can be replaced clean water, strain liquid and complex enzyme and sugar residue wet basis, thalline
One or more of acid sludge wet basis, sugar residue thalline acid sludge wet basis, citric acid acid sludge wet basis and calcium citrate acid sludge wet basis are compound
It obtains.
Heretofore described livestock and poultry acidification calcium salt glycolysis feed preferably also contains feeding organic acid, feeding inorganic acid, has
One or more of machine hydrochlorate, sugar molasse syrup and liquid fat.In the present invention, the calcium lactate, citric acid concentration
The weight of liquid and glycolysis slag dregs of rice wet basis and feeding organic acid, feeding inorganic acid, acylate, sugar molasse syrup, liquid fat
Than being preferably 100：213~798：51~199：23~38：11~19：11~19：51~95：11~19；More preferably
100:223~788:57~186:25~37:12~17:12~18:55~91:12~16.In the present invention, described feeding to have
Machine acid includes for fumaric acid, malic acid, a-ketoglutaric acid, succinic acid, tartaric acid, acetic acid, propionic acid, butyric acid, ascorbic acid, bigcatkin willow
One kind in acid, gibberellic acid, kojic acid, gluconic acid, methylene succinic acid, formic acid, benzoic acid, tartaric acid and fumaric acid and sorbic acid
Or it is a variety of.The feeding inorganic acid includes one or more in phosphoric acid, hydrochloric acid and sulfuric acid.The acylate includes formic acid
Calcium, sodium propionate, calcium propionate, sodium butyrate, sodium benzoate, sodium sorbate, potassium sorbate, potassium citrate, sodium citrate and citric acid
It is one or more in calcium.The sugar molasse syrup include sucrose, glucose, fructose, glucose syrup, maltodextrin slurry/powder,
It is one or more in fructose syrup/powder, malt syrup/powder, Sugar cane molasses and beet molasses.The liquid fat includes soybean
Oil and its oil emulsion, corn oil and its oil emulsion, palm oil and its oil emulsion, rice bran oil and its oil emulsion, coconut oil and its breast
Carburetion, fish oil and animal oil and its oil emulsion, peanut oil and its oil emulsion, rapeseed oil and its oil emulsion, tea oil and its oil emulsion,
It is one or more in plants essential oil.
In order to describe in more detail the present invention, livestock and poultry provided by the invention acidification calcium salt glycolysis is raised with reference to embodiment
The preparation method of material illustrates, but cannot they be interpreted as limiting the scope of the present invention.
Embodiment 1
A kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed, includes the following steps：
150kg is cracked rice crushing, 1050kg water is added to size mixing, 0.18kg α-amylases, carbohydrase enzymolysis are added when being warming up to 95 DEG C
40kg sugar residues wet basis and 1157kg are made after separation of solid and liquid without slag saccharified liquid for liquefying-saccharifying；No slag saccharified liquid high-temperature sterilization cooling
20kg lactic acid producing strain liquids are accessed afterwards, after 49 DEG C of conditions flow down and add calcium hydroxide solution control pH6.0, anaerobic fermentation 55h, system
Obtain the calcium lactate fermentation solution of 1245kg mycetomes and lactic acid.
After the calcium lactate fermentation solution of 1245kg mycetomes and lactic acid is warming up to 80 DEG C, using solid-liquid separating equipment, it will contain
The calcium lactate fermentation solution of thalline is separated by solid-liquid separation, and 30kg thalline acid sludge wet basis and 1206kg calcium lactate thin liquids is made；By 1206kg breasts
After sour calcium thin liquid is concentrated by evaporation, 270kg lactic acid concentrated calcium solutions are prepared；
By 100kg crush maizes, after adding 200kg water to size mixing, it is added plus 0.19kg α-amylases, carbohydrase digests, be made
300kg band slag saccharified liquids.In 300kg band slag saccharified liquids, the aspergillus niger strain liquid 30kg of access production citric acid, in 30 DEG C, hair
Under the conditions of ferment pH is natural, carry out aerobic fermentation 72h, the crude citric acid fermentation broths of 328kg are made.90kg is made through being separated by solid-liquid separation
Citric acid acid sludge wet basis and 236kg citric acid thin liquids；After 236kg citric acids thin liquid is directly concentrated, 121kg citric acids are prepared
Concentrate；
40kg sugar residues wet basis, 30kg thalline acid sludges wet basis, 90kg citric acid acid sludge wet basis and 100kg dregs of beans, 30kg are done
Water purification, 20kg strain liquids, 0.09kg complex enzymes are uniformly mixed, aerobic fermentation 66h under the conditions of 27 DEG C, and 308kg glycolysis is made and raises
Expect wet basis.
System is dried after 270kg lactic acid concentrated calcium solution, 121kg citric acids concentrate and 308kg glycolysis feed wet basis are mixed
Obtain 320kg livestock and poultry acidifying lactic acid calcium glycolysis feed butts.
Embodiment 2
A kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed, includes the following steps：
By 150kg crush maizes, 1050kg water is added to size mixing, 0.16kg α-amylases, carbohydrase enzymolysis are added when being warming up to 95 DEG C
120kg sugar residues wet basis and 1080kg are made after separation of solid and liquid without slag saccharified liquid for liquefying-saccharifying；No slag saccharified liquid high-temperature sterilization cooling
50kg lactic acid producing strain liquids are accessed afterwards, after 48 DEG C of conditions flow down and add calcium hydroxide solution control pH5.9, anaerobic fermentation 55h, system
Obtain the calcium lactate fermentation solution of 1120kg mycetomes and lactic acid.
After the calcium lactate fermentation solution of 1120kg mycetomes and lactic acid is warming up to 80 DEG C, using solid-liquid separating equipment, it will contain
The calcium lactate fermentation solution of thalline is separated by solid-liquid separation, and 30kg thalline acid sludge wet basis and 1080kg calcium lactate thin liquids is made；By 1080kg breasts
After sour calcium thin liquid is concentrated by evaporation, 235kg lactic acid concentrated calcium solutions are prepared；
By 500kg crush maizes, after adding 1000kg water to size mixing, it is added plus 1.0kg α-amylases, carbohydrase digests, be made
1500kg band slag saccharified liquids.In 1500kg band slag saccharified liquids, the aspergillus niger strain liquid 180kg of access production citric acid, in 28-32
DEG C, fermentation pH it is natural under the conditions of, carry out aerobic fermentation 72h, 1620kg crude citric acid fermentation broths be made.It is made through being separated by solid-liquid separation
Obtain 280kg citric acid acid sludge wet basis and 1620kg citric acid thin liquids；After 1620kg citric acids thin liquid is directly concentrated, prepare
600kg citric acid concentrates；
By 120kg sugar residues wet basis, 30kg thalline acid sludges wet basis, 280kg citric acid acid sludge wet basis and 50kg dregs of beans, 15kg bacterium
Kind liquid, 0.08-0.10kg complex enzymes are uniformly mixed, aerobic fermentation 60-72h under the conditions of 25-30 DEG C, and 378kg glycolysis is made and raises
Expect wet basis.
System is dried after 235kg lactic acid concentrated calcium solution, 600kg citric acids concentrate and 378kg glycolysis feed wet basis are mixed
Obtain 670kg livestock and poultry acidifying lactic acid calcium glycolysis feed butts.
Embodiment 3
A kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed, includes the following steps：
500kg is cracked rice crushing, 3500kg water is added to size mixing, 1.20kg α-amylases, carbohydrase enzymolysis are added when being warming up to 95 DEG C
380kg sugar residues wet basis and 3620kg are made after separation of solid and liquid without slag saccharified liquid for liquefying-saccharifying；No slag saccharified liquid high-temperature sterilization cooling
100kg lactic acid producing strain liquids are accessed afterwards, flow down in 48-50 DEG C of condition plus calcium hydroxide solution controls pH6.1, anaerobic fermentation 55h
Afterwards, the calcium lactate fermentation solution of 3710kg mycetomes and lactic acid is made.
After the calcium lactate fermentation solution of 3710kg mycetomes and lactic acid is warming up to 80 DEG C, using solid-liquid separating equipment, it will contain
The calcium lactate fermentation solution of thalline is separated by solid-liquid separation, and 120kg thalline acid sludge wet basis and 3590kg calcium lactate thin liquids is made；By 3590kg breasts
After sour calcium thin liquid is concentrated by evaporation, 990kg lactic acid concentrated calcium solutions are prepared；
By 100kg crush maizes, after adding 200kg water to size mixing, it is added plus 0.17kg α-amylases, carbohydrase digests, be made
300kg band slag saccharified liquids.In 300kg band slag saccharified liquids, the aspergillus niger strain liquid 30kg of access production citric acid, in 29 DEG C, hair
Under the conditions of ferment pH is natural, carry out aerobic fermentation 72h, the crude citric acid fermentation broths of 328kg are made.90kg is made through being separated by solid-liquid separation
Citric acid acid sludge wet basis and 236kg citric acid thin liquids；After 236kg citric acids thin liquid is directly concentrated, 121kg citric acids are prepared
Concentrate；
By 380kg sugar residues wet basis, 120kg thalline acid sludges wet basis, 90kg citric acid acid sludge wet basis and 100kg dregs of beans, 30kg
Strain liquid, 0.08kg complex enzymes are uniformly mixed, and 700kg glycolysis feed wet basis is made in aerobic fermentation 60-72h under the conditions of 28 DEG C.
It is dried after 980-1000kg lactic acid concentrated calcium solution, 121kg citric acids concentrate and 700kg glycolysis feed wet basis are mixed
It is drying to obtain 665-675kg livestock and poultry acidifying lactic acid calcium glycolysis feed butts.
Embodiment 4
A kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed, includes the following steps：
By 500kg Cassava crushings, 3500kg water is added to size mixing, 1.38kg α-amylases, carbohydrase enzymolysis are added when being warming up to 95 DEG C
450kg sugar residues wet basis and 3550kg are made after separation of solid and liquid without slag saccharified liquid for liquefying-saccharifying；No slag saccharified liquid high-temperature sterilization cooling
80kg lactic acid producing strain liquids are accessed afterwards, flow down in 48-50 DEG C of condition plus calcium hydroxide solution controls pH5.8-6.2, anaerobic fermentation
After 72h, the calcium lactate fermentation solution of 3635kg mycetomes and lactic acid is made.
After the calcium lactate fermentation solution of 3635kg mycetomes and lactic acid is warming up to 80 DEG C, using solid-liquid separating equipment, it will contain
The calcium lactate fermentation solution of thalline is separated by solid-liquid separation, and 105kg thalline acid sludge wet basis and 3528kg calcium lactate thin liquids is made；By 3528kg breasts
After sour calcium thin liquid is concentrated by evaporation, 920-950kg lactic acid concentrated calcium solutions are prepared；
By 200kg crush maizes, after adding 500kg water to size mixing, it is added plus 0.16kg α-amylases, carbohydrase digests, be made
700kg band slag saccharified liquids.700kg band slag saccharified liquid in, access production citric acid aspergillus niger strain liquid 60kg, 28-32 DEG C,
Under the conditions of the pH that ferments is natural, carry out aerobic fermentation 60h, the crude citric acid fermentation broths of 760kg are made.It is made through being separated by solid-liquid separation
180kg citric acid acid sludge wet basis and 565kg citric acid thin liquids；After 565kg citric acids thin liquid is directly concentrated, 245kg is prepared
Citric acid concentrate；
By 450kg sugar residues wet basis, 105kg thalline acid sludges wet basis, 180kg citric acid acid sludge wet basis and 100kg dregs of beans, 80kg
Strain liquid, 0.087kg complex enzymes are uniformly mixed, and 915-920kg glycolysis is made in aerobic fermentation 48-60h under the conditions of 25-30 DEG C
Feed wet basis.
920-950kg lactic acid concentrated calcium solution, 245kg citric acids concentrate and 915-920kg glycolysis feed wet basis are mixed
It dries afterwards and 780-785kg livestock and poultry acidifying lactic acid calcium glycolysis feed butts is made.
Embodiment 5
A kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed, includes the following steps：
By 500kg molasses ion-exchange deferrizations, adds 1000kg water to size mixing, 1.18kg α-amylases, carbohydrase are added when being warming up to 95 DEG C
Enzymatic liquefaction is saccharified, and 60kg lactic acid producing strain liquids are accessed after high-temperature sterilization cooling, flows down in 42-45 DEG C of condition plus calcium hydroxide is molten
After hydraulic control pH5.8-6.2, anaerobic fermentation 96h, the calcium lactate fermentation solution of 1485kg mycetomes and lactic acid is made.
After the calcium lactate fermentation solution of 1485kg mycetomes and lactic acid is warming up to 80 DEG C, using solid-liquid separating equipment, it will contain
The calcium lactate fermentation solution of thalline is separated by solid-liquid separation, and 280kg thalline acid sludge wet basis and 1202kg calcium lactate thin liquids is made；By 1202kg breasts
After sour calcium thin liquid is concentrated by evaporation, 300-320kg lactic acid concentrated calcium solutions are prepared；
By 300kg crush maizes, after adding 700kg water to size mixing, it is added plus 0.54kg α-amylases, carbohydrase digests, be made
1000kg band slag saccharified liquids.In 1000kg band slag saccharified liquids, the aspergillus niger strain liquid 100kg of access production citric acid, in 28-32
DEG C, fermentation pH it is natural under the conditions of, carry out aerobic fermentation 72h, 1180kg crude citric acid fermentation broths be made.It is made through being separated by solid-liquid separation
Obtain 280kg citric acid acid sludge wet basis and 900kg citric acid thin liquids；After 900kg citric acids thin liquid is directly concentrated, prepare
365kg citric acid concentrates；
By 280kg thalline acid sludges wet basis, 280kg citric acid acid sludge wet basis and 100kg corn protein powders, 50kg strain liquids,
0.15-0.20kg complex enzymes are uniformly mixed, aerobic fermentation 72h under the conditions of 25-30 DEG C, and it is wet that 695-705kg glycolysis feeds are made
Base.
300-320kg lactic acid concentrated calcium solution, 365kg citric acids concentrate and 695-705kg glycolysis feed wet basis are mixed
It dries afterwards and 640-645kg livestock and poultry acidifying lactic acid calcium glycolysis feed butts is made.
Embodiment 6
A kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed, includes the following steps：
By 200kg crush maizes, after adding 800kg water to size mixing, it is added plus 0.46kg α-amylases, carbohydrase digests, be made
1000kg band slag saccharified liquids.In 1000kg band slag saccharified liquids, the aspergillus niger strain liquid 60kg of access production citric acid, in 28-32
DEG C, fermentation pH it is natural under the conditions of, carry out aerobic fermentation 72h, 1045kg crude citric acid fermentation broths be made.It is crude in 1045kg
35kg calcium carbonate is added in citric acid fermentation, in not exclusively and under conditions of generate 85kg calcium citrates precipitation, through solid-liquid point
It is from obtained 315kg calcium citrates acid sludge wet basis (calcium citrate containing 85kg) and 745kg citric acid thin liquids, 745kg citric acids is dilute
Liquid is concentrated by evaporation into 230kg citric acid concentrates；
By 315kg calcium citrate acid sludge wet basis and 100kg dregs of beans, 30kg clean waters, 30kg strain liquids, 0.20-0.30kg
Complex enzyme is uniformly mixed, aerobic fermentation 60-72h under the conditions of 25-30 DEG C, and 458-462kg glycolysis feed wet basis is made.
It is dried after 230kg citric acids concentrate and 458-462kg glycolysis feed wet basis are mixed and 275-282kg livestock and poultry is made
It is acidified calcium citrate glycolysis feed butt.
Embodiment 7
A kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed, includes the following steps：
By 200kg crush maizes, after adding 800kg water to size mixing, it is added plus 0.38kg α-amylases, carbohydrase digests, be made
1000kg band slag saccharified liquids.In 1000kg band slag saccharified liquids, the aspergillus niger strain liquid 60kg of access production citric acid, in 28-32
DEG C, fermentation pH it is natural under the conditions of, carry out aerobic fermentation 72h, 1045kg crude citric acid fermentation broths be made, made through being separated by solid-liquid separation
Obtain 185kg citric acid acid sludge wet basis and 960kg citric acid thin liquids；25kg calcium hydroxides are added in 960kg citric acid thin liquids,
75kg calcium citrates precipitation is generated under conditions of not exclusively neutralizing, and 75kg calcium citrates and 885kg lemons is made through being separated by solid-liquid separation
Thin liquid after acid neutralizes, thin liquid is concentrated by evaporation into 230kg citric acid concentrates after 885kg citric acids are neutralized；
185kg citric acid acid sludge wet basis and 100kg dregs of beans, 40kg clean waters, 30kg strain liquids, 0.20-0.30kg is multiple
Synthase is uniformly mixed, aerobic fermentation 60-72h under the conditions of 25-30 DEG C, and 345-350kg glycolysis feed wet basis is made.
It dries and is made after 230kg citric acids concentrate, 75kg calcium citrates and 345-350kg glycolysis feed wet basis are mixed
273-280kg livestock and poultry are acidified calcium citrate glycolysis feed butt.
Embodiment 8
A kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed, includes the following steps：
By 200kg crush maizes, after adding 800kg water to size mixing, it is added plus 0.41kg α-amylases, carbohydrase digests, be made
1000kg band slag saccharified liquids.In 1000kg band slag saccharified liquids, the aspergillus niger strain liquid 60kg of access production citric acid, in 28-32
DEG C, fermentation pH naturally with stream plus calcium hydroxide and not exclusively neutralize under conditions of, carry out aerobic fermentation 72h, be made 1045kg it is thick
265kg calcium citrate acid sludge wet basis (calcium citrate containing 65kg) is made through being separated by solid-liquid separation in calcium citrate and citric acid fermentation broth processed
With 780kg citric acid thin liquids, 780kg citric acid thin liquids are concentrated by evaporation into 250kg citric acid concentrates；
By 265kg calcium citrate acid sludge wet basis and 100kg dregs of beans, 35kg clean waters, 25kg strain liquids, 0.20-0.30kg
Complex enzyme is uniformly mixed, aerobic fermentation 60-72h under the conditions of 25-30 DEG C, and 400-405kg glycolysis feed wet basis is made.
It is dried after 250kg citric acids concentrate and 400-405kg glycolysis feed wet basis are mixed and 270-280kg livestock and poultry is made
It is acidified calcium citrate glycolysis feed butt.
Embodiment 9
A kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed, includes the following steps：
150kg is cracked rice crushing, 1050kg water is added to size mixing, 0.19kg α-amylases, carbohydrase enzymolysis are added when being warming up to 95 DEG C
40kg sugar residues wet basis and 1157kg are made after separation of solid and liquid without slag saccharified liquid for liquefying-saccharifying；No slag saccharified liquid high-temperature sterilization cooling
20kg lactic acid producing strain liquids are accessed afterwards, flow down in 48-50 DEG C of condition plus calcium hydroxide solution controls pH5.8-6.2, anaerobic fermentation
After 55h, the calcium lactate fermentation solution of 1245kg mycetomes and lactic acid is made.
After the calcium lactate fermentation solution of 1245kg mycetomes and lactic acid is warming up to 80 DEG C, using solid-liquid separating equipment, it will contain
The calcium lactate fermentation solution of thalline is separated by solid-liquid separation, and 30kg thalline acid sludge wet basis and 1206kg calcium lactate thin liquids is made；By 1206kg breasts
After sour calcium thin liquid is concentrated by evaporation, 266-276kg lactic acid concentrated calcium solutions are prepared；
By 200kg crush maizes, after adding 800kg water to size mixing, it is added plus 0.38kg α-amylases, carbohydrase digests, be made
1000kg band slag saccharified liquids.1000kg band slag saccharified liquid in, access production citric acid aspergillus niger strain liquid 60kg, 30 DEG C,
Under the conditions of the pH that ferments is natural, carry out aerobic fermentation 72h, the crude citric acid fermentation broths of 1045kg are made.In the crude lemons of 1045kg
In lemon acid fermentation be added 35kg calcium carbonate, not exclusively in and under conditions of generate 85kg calcium citrates precipitation, through be separated by solid-liquid separation
315kg calcium citrate acid sludge wet basis (calcium citrate containing 85kg) and 745kg citric acid thin liquids is made, by 745kg citric acid thin liquids
It is concentrated by evaporation into 230kg citric acid concentrates；
By 40kg sugar residues wet basis, 30kg thalline acid sludges wet basis, 315kg calcium citrate acid sludge wet basis and 100kg dregs of beans, 30kg
Clean water, 30kg strain liquids, 0.23kg complex enzymes are uniformly mixed, and 525- is made in aerobic fermentation 60-72h under the conditions of 25-30 DEG C
535kg glycolysis feed wet basis.
266-276kg lactic acid concentrated calcium solution, 230kg citric acids concentrate and 525-535kg glycolysis feed wet basis are mixed
It dries afterwards and 275-282kg livestock and poultry acidifying lactic acid calcium and calcium citrate glycolysis feed butt is made.
One, main active substances contain in the livestock and poultry acidification calcium salt glycolysis feed that prepared by embodiment 1, embodiment 2 and embodiment 3
Amount, see the table below 1,2,3.
Nutrition content in livestock and poultry acidification calcium salt glycolysis feed product prepared by table 1, embodiment 1
Nutrition content in livestock and poultry acidification calcium salt glycolysis feed product prepared by table 2, embodiment 2
Nutrition content in livestock and poultry acidification calcium salt glycolysis feed product prepared by table 3, embodiment 3
Two, the feeding weanling pig effect for the livestock and poultry acidification calcium salt glycolysis feed that prepared by embodiment 1.
To inquire into the feeding effect that livestock and poultry prepared by embodiment 1 are acidified calcium salt glycolysis feed to weanling pig, choose 23
Age Ternary Pig three way cross sodium selenite 450, close by weight, parity, the fifty-fifty principle of gender is randomly divided into 3 groups, every group
15 repetitions each repeat 10 pigs.Control group does not add acidulant, and using mountain flour as calcium source.Acidulant group adds commercially available acid
6kg/ tons of agent, and using mountain flour as calcium source.Test group adds 30kg/ tons of livestock and poultry acidification calcium salt glycolysis feed, and according to calcium content
Mountain flour is substituted on year-on-year basis, makes weaning pigs.Each group feed protein and energy are close.Piglet is weaned for 23 days, experimental period 30
It.Test result is as follows：
The influence of livestock and poultry acidification calcium salt glycolysis feed prepared by table 4, embodiment 1 to Production Performance of Weaning Pigs
| Project | Control group | Acidulant group | 30kg/ tons of groups |
| Daily gain (g) | 372.45 | 390.09 | 414.69 |
| Daily ingestion amount (g) | 554.95 | 565.63 | 584.71 |
| Diarrhea rate/% | 47.11 | 18.88 | 3.33 |
| Feedstuff-meat ratio | 1.49 | 1.45 | 1.41 |
Commercially available acidulant 6kg/ tons are added in daily ration, after 30kg/ tons of livestock and poultry acidification calcium salt glycolysis feed, the day of weanling pig
Weightening improves 4.74% and 11.34% than control group respectively, and diarrhea rate reduces by 59.92% and 92.93% than control group respectively, material
Meat reduces by 2.69% and 5.37% than respectively than control group, and feed intake improves 1.92% and 5.36% than control group respectively.As a result
Show：Livestock and poultry acidification calcium salt glycolysis feed can significantly improve the production performance of weanling pig, and feeding effect be better than it is commercially available
Acidulant.
Three, the feeding grower pigs effect for the livestock and poultry acidification calcium salt glycolysis feed that prepared by embodiment 2.
Calcium salt glycolysis feed is acidified to the feeding effect of grower pigs, selective body heavy phase to inquire into livestock and poultry prepared by embodiment 2
The closely Ternary Pig ternary grower pigs 300 of (30.0 ± 1.0kg of weight), are randomly divided into control group, acidulant group, test group.Three
Group grower pigs weight is close (significance analysis is not notable), and each group is all provided with 10 repetitions, each to repeat 10.
Three groups of grower pigs basal diet trophic level all sames, are powder.Control group does not add acidulant, and with mountain flour
For calcium source.Commercially available acidulant 3kg/ tons of acidulant group addition, and using mountain flour as calcium source.Test group adds livestock and poultry and is acidified calcium salt glycolysis
10kg/ tons of feed, and mountain flour is substituted on year-on-year basis according to calcium content, make growth pig feed.Each group feed protein and energy are close.
Test preliminary trial period：The prerun since 21 days March in 2016 is tested, 7 days preliminary trial periods, carries out prevention, inoculation, expelling parasite etc.
Work enters positive examination after prerun.
Test the pre- positive examination phase：Since on March 28th, 2016 is formal, on May 2nd, 2016 formally terminates, and lasts 35 for experiment
It.Test result is as follows：
The influence of livestock and poultry acidification calcium salt glycolysis feed prepared by table 5, embodiment 2 to growth daily gain in pigs
| Project | Control group | Acidulant group | 30kg/ tons of groups |
| Daily gain (g) | 612.21 | 629.75 | 662.73 |
| Daily ingestion amount (g) | 1316.25 | 1328.77 | 1365.22 |
| Feedstuff-meat ratio | 2.15 | 2.11 | 2.06 |
Commercially available acidulant 3kg/ tons are added in daily ration, after 10kg/ tons of livestock and poultry acidification calcium salt glycolysis feed, grower pigs increase day by day
2.87% and 8.25% is improved than control group respectively again, feedstuff-meat ratio reduces by 1.86% and 4.18% than control group respectively, feed intake
Respectively 0.95% and 3.72% is improved than control group.The result shows that：Livestock and poultry acidification calcium salt glycolysis feed can significantly improve growth
The production performance of pig, and feeding effect is better than commercially available acidulant.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed, includes the following steps：
Calcium source is mixed with citric acid concentrate, obtains livestock and poultry acidification calcium salt glycolysis feed, the calcium source is calcium lactate and/or lemon
Lemon acid calcium；
The preparation method of the citric acid concentrate includes：
Starch-containing product is crushed or sugar-containing product is beaten, obtains enzymolysis raw material；The enzymolysis raw material is mixed with water and is sized mixing, is added
Band slag saccharified liquid is made in amylase enzymolysis liquefying-saccharifying；
After accessing 29~31 DEG C of 62~70h of fermentation of production citric acid strain to the band slag saccharified liquid, centrifugation or filtering fermentating liquid are received
Integrate liquid phase component as citric acid thin liquid, solid phase components are citric acid acid sludge wet basis, are concentrated by evaporation the citric acid thin liquid, obtain lemon
Lemon acid concentrate；
The preparation method of the calcium citrate includes：
Stream plus calcium hydroxide or calcium carbonate in above-mentioned citric acid concentration liquid and preparation method thereof access production citric acid strain fermentation process,
Centrifugation or filtering fermentating liquid, collection solid phase components are calcium citrate acid sludge wet basis, contain lemon in the calcium citrate acid sludge wet basis
Lemon acid calcium；
Or by the citric acid thin liquid and calcium hydroxide or calcium carbonate in not exclusively and under conditions of mix, collection solid phase group
It is divided into calcium citrate；
The preparation method of the calcium lactate includes：
Starch-containing product is crushed or sugar-containing product is beaten, obtains enzymolysis raw material；The enzymolysis raw material is mixed with water and is sized mixing, is added
Band slag saccharified liquid is made in amylase enzymolysis, liquefaction, saccharification；
To after 48~50 DEG C of 24~58h of fermentation of band slag saccharified liquid access lactic acid producing strain, centrifugation or filtering fermentating liquid are collected
Liquid phase component is calcium lactate；Solid phase components are sugar residue thalline acid sludge wet basis；
Or be separated by solid-liquid separation the band slag saccharified liquid, it is no slag saccharified liquid to collect liquid phase component, and solid phase components are sugar residue wet basis,
To after 24~58h of the no slag saccharified liquid access lactic acid producing strain fermentation, centrifugation or filtering fermentating liquid, it is breast to collect liquid phase component
Sour calcium, solid phase components are thalline acid sludge wet basis；
Or by molasses ion-exchange deferrization, adds water to size mixing obtained liquid molasses, lactic acid producing strain fermentation 24 is accessed into the liquid molasses
After~58h, centrifugation or filtering fermentating liquid, collection liquid phase component are calcium lactate.
2. the preparation method of livestock and poultry acidification calcium salt glycolysis feed according to claim 1, which is characterized in that the livestock and poultry acid
Change calcium salt glycolysis feed and also contain glycolysis slag dregs of rice wet basis, the glycolysis slag dregs of rice wet basis after glycolysis raw material enzymatic hydrolysis and fermentation by will obtain
, the glycolysis raw material includes feedstuff, clean water, strain liquid and complex enzyme；The enzymatic hydrolysis and fermentation temperature is 34~37 DEG C,
Enzymatic hydrolysis and fermentation initial ph value is 6.8,81~91h of total time of the enzymatic hydrolysis and fermentation.
3. the preparation method of livestock and poultry acidification calcium salt glycolysis feed according to claim 2, which is characterized in that the glycolysis is former
Material further includes that sugar residue wet basis, thalline acid sludge wet basis, sugar residue thalline acid sludge wet basis, citric acid acid sludge wet basis and calcium citrate acid sludge are wet
One or more of base.
4. the preparation method of livestock and poultry acidification calcium salt glycolysis feed according to claim 2, which is characterized in that the glycolysis is former
Material replaces with clean water, strain liquid and complex enzyme and sugar residue wet basis, thalline acid sludge wet basis, sugar residue thalline acid sludge wet basis, citric acid
The compound acquisition of one or more of acid sludge wet basis and calcium citrate acid sludge wet basis.
5. the preparation method of livestock and poultry acidification calcium salt glycolysis feed according to claim 2, which is characterized in that the livestock and poultry acid
Change calcium salt glycolysis feed also to contain in feeding organic acid, feeding inorganic acid, acylate, sugar molasse syrup and liquid fat
It is one or more of.
6. a kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed according to claim 2, which is characterized in that the feeding
Expect that raw material is the one or more of feedstuff in Minister Agriculture of China's feedstuff catalogue, including dregs of beans, cotton dregs, rapeseed dregs, flower
It is the raw dregs of rice, fish meal, blood meal, plasma proteins, hyperglobulinemia, milk powder, lactose, whey powder, wheat gluten flour, corn protein powder, big
Rice gluten powder DDGS, soybean, pea, red bean, corn, paddy, cracks rice, is wheat, wheat bran, rice bran, flour, oat, barley, red
Potato, soybean protein isolate, soy protein concentrate, pasture powder, Chinese herbal medicine, fruit and vegetable and sugar molasse syrup or above-mentioned feeding
Expect one or more in the dilated product of raw material；
The strain liquid includes lactobacillus inoculation liquid, barms liquid, bacillus subtilis strain liquid, bacillus licheniformis kind liquid, double
It is one or more in discrimination bacillus specie liquid, aspergillus oryzae kind liquid, Brevibacterium acetylicum kind liquid and clostridium tyrobutyricum kind liquid；
The complex enzyme includes protease, zytase, dextranase, mannonase pectase, α-amylase, carbohydrase, sugar
Change a variety of in enzyme and cellulase；
The feeding organic acid or its concentrate include for fumaric acid, malic acid, a-ketoglutaric acid, succinic acid, tartaric acid, second
Acid, propionic acid, butyric acid, ascorbic acid, salicylic acid, gibberellic acid, kojic acid, gluconic acid, methylene succinic acid, formic acid, benzoic acid, winestone
It is one or more in acid and fumaric acid and sorbic acid；
The feeding inorganic acid includes one or more in phosphoric acid, hydrochloric acid and sulfuric acid；
The acylate includes calcium formate, sodium propionate, calcium propionate, sodium butyrate, sodium benzoate, sodium sorbate, potassium sorbate, lemon
It is one or more in lemon acid potassium, sodium citrate and calcium citrate；
The sugar molasse syrup include sucrose, glucose, fructose, glucose syrup, maltodextrin slurry/powder, fructose syrup/powder,
It is one or more in malt syrup/powder, Sugar cane molasses and beet molasses；
The liquid fat includes soybean oil and its oil emulsion, corn oil and its oil emulsion, palm oil and its oil emulsion, rice bran oil
And its oil emulsion, coconut oil and its oil emulsion, fish oil and animal oil and its oil emulsion, peanut oil and its oil emulsion, rapeseed oil and
It is one or more in its oil emulsion, tea oil and its oil emulsion, plants essential oil.
7. a kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed according to claim 1, which is characterized in that the production
The fermentation pH of citric acid fungus strain is nature pH；Fermented type is aerobic fermentation；Fermentation time is 63~69h；
The fermentation pH value of the lactic acid producing strain fermentation is 3~9；Fermented type is anaerobic fermentation；Fermentation time is 50~56h.
8. a kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed according to claim 3, which is characterized in that the sugar
Slag wet basis, thalline acid sludge wet basis/sugar residue thalline acid sludge wet basis, citric acid acid sludge wet basis and feedstuff, clean water, strain liquid,
The weight ratio of complex enzyme is 100：0~70：0~38：220~789：0~186：12~19：0.008~1.9.
9. a kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed according to claim 6, which is characterized in that the bacterium
Lactic acid bacteria culturers liquid in kind liquid：Yeast seeds liquid：The number of viable ratio of Bacillus licheniformis strain liquid is 100：11~28：
6~9；
Protease in the complex enzyme：Zytase：Dextranase：Mannase：The weight ratio of pectase is 100：62
~78：61~79：63~78：31~45.
10. a kind of preparation method of livestock and poultry acidification calcium salt glycolysis feed according to claim 5, which is characterized in that described
Calcium lactate, citric acid concentrate and glycolysis slag dregs of rice wet basis and feeding organic acid, feeding inorganic acid, acylate, sugar molasse sugar
It starches, the weight ratio of liquid fat is 100：213~798：51~199：23~38：11~19：11~19：51~95：11~19.
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| US11856969B2 (en) | 2020-05-01 | 2024-01-02 | Packerland Whey Products Inc. | Compositions and methods for solidified fermented animal feed |
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