CN108728103A - The extracting method of geobiont element - Google Patents
The extracting method of geobiont element Download PDFInfo
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- CN108728103A CN108728103A CN201810345077.0A CN201810345077A CN108728103A CN 108728103 A CN108728103 A CN 108728103A CN 201810345077 A CN201810345077 A CN 201810345077A CN 108728103 A CN108728103 A CN 108728103A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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Abstract
Extracting method of the present invention about a kind of geobiont element, includes the following steps：Feedstock capture：Acquire a soil raw material containing viable bacteria and biological enzyme；Biological eutrophication is cultivated：The soil raw material and a nutrient solution are uniformly mixed to form a mixed raw material and cultivate a predetermined time to increase number of viable and biological enzyme concentration；It raises and train：The low temperature that the mixed raw material sequentially carries out being not higher than 10 DEG C is raised and train, the high temperature not less than 50 DEG C is raised and train, high dissolved oxygen of the dissolved oxygen (Dissolved Oxygen, DO) not less than 5.0mg/L is raised and train and low dissolved oxygens of the DO less than 0.5mg/L is raised and train；Extraction：The liquid portion for extracting the mixed raw material after raising and train is geobiont element.
Description
Technical field
The present invention is related with soil fertility activation, in particular to a kind of extracting method of geobiont element.
Background technology
With the development of industry, improper throw aside of the industrial wastewater of plant emissions, exhaust gas and waste makes pollutant adsorb
Or penetrate into soil, accelerate soil productivity to degenerate make barren and is unfavorable for plant growth.It is excessive to use chemical fertilizer and not
When using the insufficient organic fertilizer of fermentation maturity, also results in nitrogen content or salinity is excessively high and make crops that can not grow or contain
The ingredient of harmful to human.Originally there is the ability of self purification so because Ecological Distribution of Soil Microorganisms is destroyed totally, to make it in soil
Purification rate far away from pollution rate, cause part farmland soil fertility to be lost, fertilising it is invalid, face the problem of can not continuing farming.
Therefore, it is necessary to the extracting method of geobiont element a kind of novel and with progressive is provided, it is above-mentioned to solve
The problem of.
Invention content
The main purpose of the present invention is to provide a kind of extracting method of geobiont element, it can concentrate and form natural soil life
Object element, quickly to supplement due microorganism species in soil, various catabolic enzymes and biologic ferment, and then can be in a short time
Soil fertility is promoted, soil activation is increased and improves fertilising efficiency.
To reach above-mentioned purpose, the present invention provides a kind of extracting method of geobiont element, includes the following steps：Raw material is adopted
Collection：Acquire a soil raw material containing viable bacteria and biological enzyme；Biological eutrophication is cultivated：The soil raw material and a nutrient solution is equal
It is even to be mixed to form a mixed raw material and cultivate a predetermined time to increase viable bacteria amount and biological enzyme concentration；It raises and train：The mixed raw material
Sequentially carry out raising and train not higher than 10 DEG C of low temperature, the high temperature not less than 50 DEG C is raised and train, dissolved oxygen (Dissolved Oxygen, DO) no
High dissolved oxygen less than 5.0mg/L is raised and train and low dissolved oxygens of the DO less than 0.5mg/L is raised and train；Extraction：The mixing after extraction is raised and train is former
The liquid portion of material is geobiont element.
Further, which cultivates in step, which includes LB powder
(LysogenyBroth), agar (Agar), yeast extract (Yeast Extract), micro comprehensive amino acid and water, the mixing are former
Living bacteria count after material culture is up to 1,000,000,000 every gram or more, effective content of organic matter up to 80% or more.
Further, which is 1% to 5% altogether with the weight percent shared by the agar.
Further, which accounts for 1% to 5%.
Further, which accounts for 0.1% to 1.0%.
Further, which raises and train to be cultivated 7 to 10 days in the environment of not higher than 10 DEG C；The high temperature raise and train for
It is cultivated 7 to 10 days in the environment of not less than 50 DEG C.
Further, which raises and train to be cultivated 7 to 10 days in the environment of dissolved oxygen is not less than 5.0 milligrams every liter；
The low dissolved oxygen raises and train to cultivate 7 to 10 days in the environment of dissolved oxygen is not more than 0.5 milligram every liter.
Further, which, which cultivates, and this raises and train step needs circulating repetition operation at least three times.
Further, which can concentrate, and condensing mode includes vacuum drying, freeze-drying and solid-state biology
Property carrier adsorption one at least within.
Further, which accounts for 1% to 5%；Shared by the micro comprehensive amino acid
Weight percent is 0.1% to 1.0%；The low temperature raises and train to cultivate 7 to 10 days in the environment of not higher than 10 DEG C；The height is docile
It supports to be cultivated 7 to 10 days in the environment of not less than 60 DEG C；The high dissolved oxygen raises and train to be not less than 5.0 milligrams every liter of ring in dissolved oxygen
It is cultivated 7 to 10 days under border；The low dissolved oxygen raises and train to cultivate 7 to 10 days in the environment of dissolved oxygen is not more than 0.5 milligram every liter；The life
Object eutrophication, which is cultivated, and this raises and train step needs circulating repetition operation at least three times；The geobiont element can concentrate, condensing mode
Including vacuum drying, freeze-drying and the biological carrier adsorption of solid-state one at least within.
The beneficial effects of the present invention are：
The present invention provide geobiont element extracting method, using this method can high concentration form the geobiont element, this
The use of geobiont element can accelerate fermentation maturation and the refinement organic molecule of organic fertilizer, and it is micro- quickly to establish the balanced soil of health
Biological and ecological, and then achieve the effect that promote soil fertility, restore arable land plantation ability.The geobiont element is equally applicable for for example having
Sewage regulation in machine offal treatment, river waters, fast decoupled organic matter simultaneously reduce stink.
Description of the drawings
Fig. 1 is a preferred embodiment of the present invention flow chart.
Description of the drawings
S1：Feedstock capture；S2：Biological eutrophication is cultivated；S3：It raises and train；S31：Low temperature is raised and train；S32：High temperature is raised and train；
S33：High dissolved oxygen is raised and train；S34：Low dissolved oxygen is raised and train；S4：Extraction.
Specific implementation mode
The possible state sample implementation of the present invention is only illustrated with embodiment below, so not to be protected to limit the present invention
Scope, conjunction are first chatted bright.
Referring to FIG. 1, the preferred embodiment of its display present invention, the extracting method of geobiont of the invention element include
The following steps：
Step S1：Feedstock capture acquires a soil raw material containing viable bacteria and biological enzyme.The soil raw material preferably acquisition is certainly
Virgin forest without pollution or grassland, such as but be not limited to blackland, peat soil etc., which can preferably have life
Object enzyme (such as carbohydrate catabolic enzyme, protein decomposition enzyme and lipolytic enzyme), beneficial flora (such as fungi, radioactive ray bacterium, gemma bar
Bacterium, Filamentous bacillus, rhizobium, Penicillium notatum, aspergillus and Glan Salmonella etc.), organic acid (such as amino acid, humic acid and the quick acid of husband
Deng), the nutriments such as algae and photosynthetic bacteria group.
Step S2：Biological eutrophication is cultivated, and the soil raw material and a nutrient solution are uniformly mixed to form a mixed raw material
And a predetermined time is cultivated to increase number of viable and biological enzyme concentration.The nutrient solution includes LB powder (Lysogeny
Broth), agar (Agar), yeast extract (Yeast Extract), micro comprehensive amino acid and water, the mixed raw material culture 7 to
10 angels its living bacteria count reaches 1,000,000,000 every gram or more, it is ensured that nutrition content is sufficient for various raising and train process.Wherein,
Weight percent shared by the LB powder and agar is preferably 1% to 5% altogether；Weight percent shared by the yeast extract is preferably
1% to 5%；Weight percent shared by the micro comprehensive amino acid is preferably 0.1% to 1.0%.
Step S3：It raises and train.Step S3 includes step S31：The low temperature that the mixed raw material sequentially carries out not higher than 10 DEG C is raised and train；
Step S32：High temperature not less than 50 DEG C is raised and train；Step S33：Dissolved oxygen (Dissolved Oxygen, DO) is not less than 5.0mg/L's
High dissolved oxygen is raised and train；Step S34：Low dissolved oxygens of the DO less than 0.5mg/L is raised and train.Preferably, the low temperature is raised and train as not higher than 10 DEG C
In the environment of cultivate 7 to 10 days；The high temperature raises and train to cultivate 7 to 10 days in the environment of not less than 50 DEG C；The high dissolved oxygen is raised and train
To be cultivated 7 to 10 days in the environment of dissolved oxygen is not less than 5.0 milligrams every liter；The low dissolved oxygen raises and train to be not more than every liter in dissolved oxygen
It is cultivated 7 to 10 days in the environment of 0.5 milligram.Step S2 and step S3 needs circulating repetition operation at least three times.It thereby, can be by soil
In biotin amplification, reinforcing, screening formed with Concentrated Compact.
Step S4：Extraction, the liquid portion for extracting the mixed raw material after raising and train are geobiont element.The soil is given birth to
The living bacteria count of object element is preferably up to 1,500,000,000 every gram or more, it is ensured that bacterium amount abundance is for subsequent applications.Geobiont element
It can concentrate, condensing mode includes vacuum drying, freeze-drying and the biological carrier adsorption of solid-state one at least within, after concentration
The geobiont element can be a siccative, be readily transported and subsequent applications.So, the geobiont element after concentration also can be liquid.
The biological carrier of the solid-state can such as but not being limited to cocoanut fiber or charcoal, the mixed raw material after being raised and train with absorption.
Through the above steps, can high concentration form geobiont element, the use of geobiont element can accelerate organic fertilizer
Fermentation maturation and refinement organic molecule, quickly establish the balanced Ecological Distribution of Soil Microorganisms of health, and then reach and promote soil fertility, extensive
Plant to second ploughing the effect of ability.The geobiont element is equally applicable for the dirt in such as organic waste treatment, river waters
Water is renovated, and fast decoupled organic matter simultaneously reduces stink.
Claims (10)
1. a kind of extracting method of geobiont element, which is characterized in that include the following steps：
Feedstock capture：Acquire a soil raw material containing viable bacteria and biological enzyme；
Biological eutrophication is cultivated：The soil raw material and a nutrient solution are uniformly mixed to form a mixed raw material, culture one is predetermined
Time is to increase number of viable and biological enzyme concentration；
It raises and train：The low temperature that the mixed raw material sequentially carries out being not higher than 10 DEG C is raised and train, the high temperature not less than 50 DEG C is raised and train, dissolved oxygen is not small
It is raised and train in the high dissolved oxygen of 5.0mg/L and low dissolved oxygens of the DO less than 0.5mg/L is raised and train；
Extraction：The liquid portion for extracting the mixed raw material after raising and train is geobiont element.
2. the extracting method of geobiont element as described in claim 1, which is characterized in that the biology eutrophication cultivates step
In, which includes LB powder, agar, yeast extract, micro comprehensive amino acid and water, effective work after the mixed raw material culture
Bacterium number is up to 1,000,000,000 every gram or more, effective content of organic matter up to 80% or more.
3. the extracting method of geobiont element as claimed in claim 2, which is characterized in that shared by the LB powder and the agar
Weight percent is 1% to 5% altogether.
4. the extracting method of geobiont element as claimed in claim 2, which is characterized in that the weight percent shared by the yeast extract
Than being 1% to 5%.
5. the extracting method of geobiont element as claimed in claim 2, which is characterized in that shared by the micro comprehensive amino acid
Weight percent is 0.1% to 1.0%.
6. the extracting method of geobiont element as described in claim 1, which is characterized in that the low temperature is raised and train as not higher than 10
It is cultivated 7 to 10 days in the environment of DEG C；The high temperature raises and train to cultivate 7 to 10 days in the environment of not less than 50 DEG C.
7. the extracting method of geobiont element as described in claim 1, which is characterized in that the high dissolved oxygen raise and train for dissolved oxygen not
It is cultivated 7 to 10 days in the environment of 5.0 milligrams less than every liter；The low dissolved oxygen raises and train to be not more than 0.5 milligram every liter of ring in dissolved oxygen
It is cultivated 7 to 10 days under border.
8. the extracting method of geobiont element as described in claim 1, which is characterized in that the biology eutrophication cultivates and should
Raising and train step needs circulating repetition operation at least three times.
9. the extracting method of geobiont element as described in claim 1, which is characterized in that the geobiont element can concentrate, dense
Contracting mode includes vacuum drying, freeze-drying and the biological carrier adsorption of solid-state one at least within.
10. the extracting method of geobiont element as claimed in claim 3, which is characterized in that the weight hundred shared by the yeast extract
Divide than being 1% to 5%；The micro comprehensive amino acid accounts for 0.1% to 1.0%；The low temperature raise and train for
It is cultivated 7 to 10 days in the environment of not higher than 10 DEG C；The high temperature raises and train to cultivate 7 to 10 days in the environment of not less than 60 DEG C；It should
High dissolved oxygen raises and train to cultivate 7 to 10 days in the environment of dissolved oxygen is not less than 5.0 milligrams every liter；The low dissolved oxygen raise and train for dissolved oxygen not
It is cultivated 7 to 10 days in the environment of 0.5 milligram more than every liter；The biology eutrophication is cultivated and this is raised and train step and circulating repetition is needed to grasp
Make at least three times；The geobiont element can concentrate, and condensing mode includes that vacuum drying, freeze-drying and the biological carrier of solid-state are inhaled
It is attached to few one of which.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810345077.0A CN108728103A (en) | 2018-04-17 | 2018-04-17 | The extracting method of geobiont element |
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| CN201810345077.0A CN108728103A (en) | 2018-04-17 | 2018-04-17 | The extracting method of geobiont element |
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