CN101244955A - Biological fertilizer and manufacture method thereof - Google Patents
Biological fertilizer and manufacture method thereof Download PDFInfo
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- CN101244955A CN101244955A CNA2007100932203A CN200710093220A CN101244955A CN 101244955 A CN101244955 A CN 101244955A CN A2007100932203 A CNA2007100932203 A CN A2007100932203A CN 200710093220 A CN200710093220 A CN 200710093220A CN 101244955 A CN101244955 A CN 101244955A
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- fertilizer
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- enzymatic microorganism
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a biological fertilizer, comprising the following components and content (part by weight): 1 to 2 parts of enzyme microorganism; 20 to 40 parts of sludge; 20 to 40 parts of Artemisia slag; 10 to 20 parts of rice bran powder; 5 to 10 parts of peat; 10 to 30 parts of smoke powder. The enzyme microorganism comprises a huge bacillus M1 which is preserved by CGMCC (Bac.megatherium.M1) and the preservation number of which is NO.2267. The biological fertilizer which operates the compatibility of the enzyme microorganism with the sludge and wastes uses the characteristics that the enzyme microorganism is high in sludge treating ability and strong in compatibility of the fertilizer, pesticides and the wastes fully, thus realizing the changing of waste to valuable and providing optional organic fertilizers for agriculture and forestry production. The biological fertilizer also discloses a preparation method of the biological fertilizer.
Description
Technical field
The present invention relates to a kind of bio-fertilizer, specifically, relate to contain the bio-fertilizer and the preparation technology thereof of enzymatic microorganism.
Background technology
Mud is a kind of potential resources that the value utilized is arranged very much, and many in the world countries are all greatly developing the various technology that sludge treatment is disposed and utilized.Because the shortcoming of other sludge treatment disposal technologies, at present to the processing method of employinging of mud maximum be that dewatered sludge is carried out composting, pass through the processing of composting process, can realize sludge stabilization and innoxious.Contain plant nutrient ingredients such as a large amount of organic matters, nitrogen, phosphorus in the sewage sludge, organic content reaches 20～80%, and total nitrogen content reaches 2～8%, full phosphorus (P
2O
5) content reaches 1.5～5%, is a kind of special concentrated fertilizer.From natural circulation viewpoint, the final home to return to of mud should be a soil; Viewpoint from soil nutrient, contain the needed element of a large amount of plant-growths in the mud, its composition is also approaching with soil, and is better than soil aspect a lot, so still be that the phytology aspect considers that the soil utilization of mud all is a kind of approach that utilizes of resource utilization no matter from soil science.
The enzymatic microorganism technology is a kind of microorganism application technology that day island proper distant relative family lasts research and development in more than 50 year, and more than 20 countries (area) apply in the world at present.Enzymatic microorganism can produce the beneficial microorganism colony that the various aerobic bacterias that add the water decomposition enzyme are formed in a large number by some, mainly is made of bacterium, yeast, more than 20 bacterial strain of thread fungus three major types.Enzymatic microorganism just is being widely used in the regeneration in useless source at present.In application in practice, the effectiveness of enzymatic microorganism processing city sludge is not good enough, and with the poor compatibility of chemical fertilizer, agricultural chemicals and waste material (sweet wormwood slag, tobacco powder, chaff powder, peat, fowl and animal excrement, crop straw etc.) and usefulness, the comprehensive treating process effect is not obvious.
Summary of the invention
Technical problem to be solved by this invention provides a kind of bio-fertilizer that contains novel enzymatic microorganism, this bio-fertilizer carries out compatibility with described enzymatic microorganism and mud, waste material, make full use of this enzymatic microorganism and handle the effectiveness height of mud, compatible strong characteristic with chemical fertilizer, agricultural chemicals and waste material, realized turning waste into wealth, for farming, production of forestry provide alternative organic fertilizer.
Another technical problem to be solved by this invention provides the preparation method of described bio-fertilizer.
Bio-fertilizer of the present invention comprises following component and content (weight part):
Enzymatic microorganism 1～2
Mud 20～40
Sweet wormwood slag 20～40
Chaff powder 10～20
Peat 5～10
Tobacco powder 10～30
The preserving number that described enzymatic microorganism comprises the preservation of Chinese common micro-organisms culture presevation administrative center is the bacillus megaterium M1 (Bac.megatherium.M1) of CGMCCNO.2267.
Above-mentioned bio-fertilizer content further is optimized for:
Enzymatic microorganism 2
Mud 20
Sweet wormwood slag 20
Chaff powder 20
Peat 10
Tobacco powder 30.
The weight ratio of described bacillus megaterium M1 and enzymatic microorganism is 1～2: 5.
Bacillus megaterium M1 has been deposited in the Chinese common micro-organisms culture presevation administrative center (being called for short CGMCC) of Institute of Microorganism, Academia Sinica on November 23rd, 2007, deposit number is CGMCC NO.2267.
The feature of bacillus megaterium M1 is as follows:
(1) colonial morphology
Irregular, the column ellipse is opaque.
(2) morphological features
Gram-positive, cell is shaft-like, and size is 0.6～0.7 * 1.8nm, and the column two ends are oval gemma, and sporangiocyst expands.
(3) physio-biochemical characteristics
There are not poly--beta-hydroxy, hydrochlorate particle pH 8 in the cell.Produce acetyl methyl carbinol, the VP reacting positive, VP liquid pH is 6.3～6.7.The catalase positive, oxidase positive.The lecithinase feminine gender, reduction nitrate.In 7%NaCl and pH5.8 growth, in anaerobic culture medium, do not grow aerobiosis.Produce acid from glucose, wood sugar and N.F,USP MANNITOL, but do not produce acid from pectinose, suckling to be in the reindeer moss peptonizes reduction reaction.
Bacillus megaterium M1 and existing bacillus megaterium strain difference are that its rate of propagation is fast, the living bacteria count height, and it is remarkable to decompose Organophosphorous compounds.
Another technical problem to be solved by this invention provides the preparation technology of described bio-fertilizer.Described enzymatic microorganism and mud, wormwood artemisia slag, chaff powder, peat and tobacco powder are mixed in proportion stirring, carry out the open solid state fermentation of aerobic high-heat then, 60～70 ℃ of compost temperatures, enter drying, pulverizing, granulation, screening through transfer system again at 6～7 days time.But be back in the fermentation step again in the waste gas suction pond that produces in the drying step.
The novel enzymatic microorganism of the present invention breeds bacterium by fermentation in a large number, produces tropina, thereby the generation dynamics is very strong, render a service the very high water decomposition enzyme that adds, carry out saccharification decomposition and amino acid at short notice and decompose, generate nutrition bases such as glucose, alcohol, amino acid, the hurried propagation of radioactive rays bacterium.Enzymatic microorganism can be carried out city sludge saccharification decomposition and protein at short notice and decompose because its brute force is added the water decomposition effect, generates glucose, alcohol, amino acid etc., for beneficial microorganism in the mud provides best culture base-material; Enzymatic microorganism of the present invention can also promote the hurried propagation of the actinomycetes in the mud, a large amount of microorganisms promote the mud humic acid to form, humic acid is a polymer substance, alkali replacement amount height has good cnmplexation adsorbability, and heavy metal is had the advantages that adsorptive power is strong and selection is adsorbed, thereby form very strong attenuation effect, simultaneously, complex microorganism and the various enzyme toxicity in the mud of can degrading is killed the harmful bacterium in the mud.These particulate matter and macromolecular substance are degraded to small-molecule substance, by bacterium and fungi absorb, mineralising, reach the purpose of purification.
Peat is the fluff packing thing of plant residue formation or the humus that forms through mineralising, and it contains great number of organic matters.But long-term the stacking has the potential ecological hazard, for example causes heavy metal contamination, takies a large amount of land resources.The sweet wormwood slag belongs to the tcm product recrement, has the Chinese medicine irritating smell to produce in the combustion processes unavoidably, has influenced peripheral resident's orthobiosis.So these refuse waste materials are carried out collection and treatment not only to have an economic benefit, and environmental benefit is more arranged.
Enzymatic microorganism of the present invention is compatible strong, and comprehensive action is obvious, and it can also be used with any chemical fertilizer, agricultural chemicals and other waste material under any condition.
The traditional zymotic method adopts fermenter, and is in the majority with anaerobically fermenting, and the present microbial strains major part of China is to adopt fermentor tank propagation, and liquid state is deposited, the complete processing complexity, and the cost height, environment and condition of storage are higher.Enzymatic microorganism of the present invention is solid-state existence, open solid-state propagation, and cost is low, efficient is high, save energy, easy to use.
The preparation method of bio-fertilizer of the present invention handles waste gas, reaches the waste gas zero release substantially.Adopt the exhausting mechanical means with waste gas suction swimmer's pool, handle the SO in the discharging
2Waste gas.
The present invention is further detailed explanation below in conjunction with embodiment.
Description of drawings
Accompanying drawing is preparation technology's schema of bio-fertilizer of the present invention.
Embodiment
Embodiment
1. the separation of bacillus megaterium M1
With commercially available enzymatic microorganism 5～10g and nutrition base W-Gum 5～10g, brown sugar 5～10g and wheat bran 10～20g uniform mixing, pH6.0～7.0, humidity is controlled to be 30～35%, add the uncommon imines 0.005～0.05g of azanol or second again, 25～35 ℃ of temperature, the temperature difference is 8～10 ℃, through 2～3 days cultivation, filters out bacillus megaterium M1 with transfering loop.Described nutrition base also can comprise at least a among fish meal 3～5g, dish cake 3～5g and the polished rice chaff 35～50g.
2. the cultivation of bacillus megaterium M1
Bacillus megaterium M1 grows on following substratum:
Sucrose 50g (NH
4)
2SO
46g NgSO
42.5g (NH
4)
3SO
42g
K
2SO
42.5g CaSO
42.5g agar 1520g distilled water 1000ml pH6.0
3. the preparation of bio-fertilizer of the present invention
With the above-mentioned bacillus megaterium M1 for preparing by 1～2: 5 part by weight mix with commercially available enzymatic microorganism and promptly get the novel enzymatic microorganism of the present invention.Novel enzymatic microorganism 2kg is mixed stirring with mud 20kg, sweet wormwood slag 20kg, chaff powder 20kg, peat 10kg, tobacco powder 30kg, carry out the open solid state fermentation of aerobic high-heat, 60 ℃ of compost temperatures, 7 days time, enter drying, pulverizing, granulation, screening, test package through transfer system again.But be back in the fermentation step again in the waste gas suction pond that produces in the drying step.
The bio-fertilizer of above-mentioned preparation, is detected by environmental monitoring station with fertile as soil, and wherein heavy metal content such as lead, cadmium, chromium arsenic, mercury meet national standard far below international standard, see Table 1.
Table 1 heavy metal in soil content detection result
| The project sample number into spectrum | Plumbous | Cadmium | Chromium | Arsenic | Total mercury |
| mg/kg | mg/kg | mg/kg | mg/kg | mg/kg | |
| Soil | 2.3 | 0.01 | 17.7 | 6.0 | 0.04 |
Pollutent control criterion (GB4284-84)/(mg/kg) sees Table 2 in the agricultural mud.
Pollutent control criterion in the agricultural mud of table 2
| Project | The highest permission content (mg/kg) | |
| Sour earth pH＜6.5 | Neutrality or alkaline soil pH value 〉=6.5 | |
| Cadmium and compound thereof (in Cd) mercury and compound (in Hg) lead thereof and compound (in Pb) chromium thereof and compound (in Cr) arsenic thereof and compound (in As) thereof | 5 5 300 600 75 | 20 15 1000 1000 75 |
Above-mentioned bio-fertilizer is to be rich in organic and ecological nitrogen, phosphorus, potassium efficient organic fertilizer, melt a company limited, the precious agricultural development company limited that converges in Chongqing City as citrus, ginger base manure by Beijing group of Huiyuan Chongqing citrus industry, its citrus grows fine, production rate is fast, the fruit pol increases, mouthfeel is good, citrus, ginger ratio are with valency fertilizer, and output increases by 20%.
Bio-fertilizer of the present invention is carried out manure trial on vegetables:
Demonstrate on pumpkin, cucumber, wild cabbage, radish vegetables, test equates with fertile cost with conventional cultivation with fertile cost design.Radish mu is thrown 190 yuan of bio-fertilizer valencys of the present invention, and plant strain growth is good, and blade is light green, and drought-resistant ability is strong; Cucumber mu is thrown 260 yuan of bio-fertilizers, the plant strain growth stalwartness, and upright blade is light green, and drought resistance is strong, and precocious 3～5 days, fruit bar shaped and quality made moderate progress; Pumpkin mu is thrown 165 yuan of bio-fertilizers, the tendril stalwartness, and leaf is light green, and drought-resistant ability is strong, and fruit marketability is good, meat powder, sweet, ripening stage basically identical; Wild cabbage mu is thrown 260 yuan, and the leaf look light green, robust growth.
Investigate bio-fertilizer degrading pesticide residues function of the present invention:
Beijing area tomato, romaine lettuce test nitrate content test result shows, executes the nitrate average content significantly the is lower than nutrient chemical fertilizer disposition of etc.ing behind the bio-fertilizer of the present invention, and wherein, the tomato nitrate content reduces by 9.5%, romaine lettuce reduction by 8.1%.
The nitrate average content of using bio-fertilizer processing of the present invention in the Chinese cabbage test of Shandong is 416.6mg/kg, and the average content of using chemical fertilizer is 764.6mg/kg.
The effect that bio-fertilizer of the present invention is improved the soil:
Experiment and demonstration data according to Beijing, Shandong, use bio-fertilizer of the present invention and improved the soil organism, full nitrogen and available phosphorus content, respectively handling the soil analysis result before Tongzhou District, Beijing tomato test results shows, the soil organism that bio-fertilizer of the present invention is handled, full nitrogen and available phosphorus are respectively 1.34%, 0.106% and 82.4mg/kg, then be respectively 1.16%, 0.087% and 78.3mg/kg Deng what the nutrient chemical fertilizer was handled, three indexs all are higher than to use and wait the processing of nutrient chemical fertilizer.
Claims (5)
1. a bio-fertilizer is characterized in that, comprises following component and content (weight part):
Enzymatic microorganism 1～2
Mud 20～40
Sweet wormwood slag 20～40
Chaff powder 10～20
Peat 5～10
Tobacco powder 10～30,
The preserving number that described enzymatic microorganism comprises the preservation of Chinese common micro-organisms culture presevation administrative center is the bacillus megaterium M1 (Bac.megatherium.M1) of CGMCCNO.2267.
2. according to the described a kind of bio-fertilizer of claim 1, it is characterized in that, comprise following component and content:
Enzymatic microorganism 2
Mud 20
Sweet wormwood slag 20
Chaff powder 20
Peat 10
Tobacco powder 30.
3. according to claim 1 or 2 described a kind of bio-fertilizers, it is characterized in that the weight ratio of described bacillus megaterium M1 and enzymatic microorganism is 1～2: 5.
4. the preparation method of claim 1 and 2 described bio-fertilizers, it is characterized in that, described enzymatic microorganism and mud, wormwood artemisia slag, chaff powder, peat and tobacco powder are mixed in proportion stirring, the open solid state fermentation of aerobic high-heat then, 60～70 ℃ of compost temperatures, 6～7 days time, enter drying, pulverizing, granulation, screening through transfer system again.
5. according to the preparation method of the described bio-fertilizer of claim 4, it is characterized in that, be back in the fermentation step again in the waste gas suction pond that produces in the described drying step.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200710093220A CN101244955B (en) | 2007-12-27 | 2007-12-27 | Biological fertilizer and manufacture method thereof |
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|---|---|---|---|
| CN200710093220A CN101244955B (en) | 2007-12-27 | 2007-12-27 | Biological fertilizer and manufacture method thereof |
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|---|---|
| CN101244955A true CN101244955A (en) | 2008-08-20 |
| CN101244955B CN101244955B (en) | 2010-05-26 |
Family
ID=39945676
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101811910A (en) * | 2010-04-16 | 2010-08-25 | 邵映辉 | Humic acid bioorganic liquid manure and special strain preparation |
| CN101857510A (en) * | 2010-06-22 | 2010-10-13 | 张才建 | Special organic composite fertilizer of citrus residues of citrus and preparation method thereof |
| CN101857470A (en) * | 2010-05-26 | 2010-10-13 | 昆明光宝生物技术工程有限公司 | Method for rapidly composting sludge |
| CN102732463A (en) * | 2012-06-27 | 2012-10-17 | 重庆市瑞宝农业产业集团有限公司 | Bacillus subtilis RB and application thereof |
| CN102746061A (en) * | 2012-06-26 | 2012-10-24 | 陈金太 | Bio-fungus polypeptide mineral fertilizer synergist |
| CN102765971A (en) * | 2012-06-27 | 2012-11-07 | 重庆市瑞宝农业产业集团有限公司 | Amino acid fertilizer and preparation method thereof |
| CN104072320A (en) * | 2014-07-04 | 2014-10-01 | 重庆鑫禾生物科技有限责任公司 | Organic-inorganic compound fertilizer capable of improving content of artemisinin and preparation method thereof |
| CN104072220A (en) * | 2014-07-04 | 2014-10-01 | 重庆鑫禾生物科技有限责任公司 | Artemisia apiacea bio-organic fertilizer and preparation method thereof |
| JP2014214059A (en) * | 2013-04-26 | 2014-11-17 | 南榛原開発株式会社 | Compost production method, land improvement material production method and sludge deodorization method |
| CN105152703A (en) * | 2015-09-02 | 2015-12-16 | 广西鸿生源环保科技有限公司 | Soil improver |
| CN105647538A (en) * | 2016-01-22 | 2016-06-08 | 成都盖尔盖司生物科技有限公司 | Soil conditioner with disease and insect damage prevention function and preparation method thereof |
| CN106397060A (en) * | 2016-11-30 | 2017-02-15 | 广西尚农生物科技有限公司 | Disease-resistance-enhancing bio-organic fertilizer and preparation method thereof |
| CN106748363A (en) * | 2016-11-30 | 2017-05-31 | 广西尚农生物科技有限公司 | A kind of biological organic fertilizer containing tobacco powder and preparation method thereof |
| CN113319110A (en) * | 2021-05-18 | 2021-08-31 | 昆明理工大学 | Method for restoring heavy metal contaminated soil and increasing fertilizer by spraying waste mash of alcohol |
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| CN100439297C (en) * | 2004-10-21 | 2008-12-03 | 刘甫名 | Preparation method of zeolite organism organic mixed fertilizer |
| CN1291947C (en) * | 2005-01-27 | 2006-12-27 | 四川新能酵素菌生物工程有限公司 | Ferment bacteria organic fertilizer and its production process |
-
2007
- 2007-12-27 CN CN200710093220A patent/CN101244955B/en not_active Expired - Fee Related
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| CN101811910A (en) * | 2010-04-16 | 2010-08-25 | 邵映辉 | Humic acid bioorganic liquid manure and special strain preparation |
| CN101857470A (en) * | 2010-05-26 | 2010-10-13 | 昆明光宝生物技术工程有限公司 | Method for rapidly composting sludge |
| CN101857510B (en) * | 2010-06-22 | 2012-10-24 | 张才建 | Special organic composite fertilizer of citrus residues of citrus and preparation method thereof |
| CN101857510A (en) * | 2010-06-22 | 2010-10-13 | 张才建 | Special organic composite fertilizer of citrus residues of citrus and preparation method thereof |
| CN102746061B (en) * | 2012-06-26 | 2014-04-02 | 陈金太 | Bio-fungus polypeptide mineral fertilizer synergist |
| CN102746061A (en) * | 2012-06-26 | 2012-10-24 | 陈金太 | Bio-fungus polypeptide mineral fertilizer synergist |
| CN102765971A (en) * | 2012-06-27 | 2012-11-07 | 重庆市瑞宝农业产业集团有限公司 | Amino acid fertilizer and preparation method thereof |
| CN102765971B (en) * | 2012-06-27 | 2014-02-19 | 重庆市瑞宝农业产业集团有限公司 | Amino acid fertilizer and preparation method thereof |
| CN102732463A (en) * | 2012-06-27 | 2012-10-17 | 重庆市瑞宝农业产业集团有限公司 | Bacillus subtilis RB and application thereof |
| JP2014214059A (en) * | 2013-04-26 | 2014-11-17 | 南榛原開発株式会社 | Compost production method, land improvement material production method and sludge deodorization method |
| CN104072320A (en) * | 2014-07-04 | 2014-10-01 | 重庆鑫禾生物科技有限责任公司 | Organic-inorganic compound fertilizer capable of improving content of artemisinin and preparation method thereof |
| CN104072220A (en) * | 2014-07-04 | 2014-10-01 | 重庆鑫禾生物科技有限责任公司 | Artemisia apiacea bio-organic fertilizer and preparation method thereof |
| CN105152703A (en) * | 2015-09-02 | 2015-12-16 | 广西鸿生源环保科技有限公司 | Soil improver |
| CN105647538A (en) * | 2016-01-22 | 2016-06-08 | 成都盖尔盖司生物科技有限公司 | Soil conditioner with disease and insect damage prevention function and preparation method thereof |
| CN106397060A (en) * | 2016-11-30 | 2017-02-15 | 广西尚农生物科技有限公司 | Disease-resistance-enhancing bio-organic fertilizer and preparation method thereof |
| CN106748363A (en) * | 2016-11-30 | 2017-05-31 | 广西尚农生物科技有限公司 | A kind of biological organic fertilizer containing tobacco powder and preparation method thereof |
| CN113319110A (en) * | 2021-05-18 | 2021-08-31 | 昆明理工大学 | Method for restoring heavy metal contaminated soil and increasing fertilizer by spraying waste mash of alcohol |
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|---|---|
| CN101244955B (en) | 2010-05-26 |
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