CN108703011A - Reduce the method that cultivation of agaricus bisporus trichoderma occurs and improves yield - Google Patents
Reduce the method that cultivation of agaricus bisporus trichoderma occurs and improves yield Download PDFInfo
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- CN108703011A CN108703011A CN201810524921.6A CN201810524921A CN108703011A CN 108703011 A CN108703011 A CN 108703011A CN 201810524921 A CN201810524921 A CN 201810524921A CN 108703011 A CN108703011 A CN 108703011A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
- A01G18/22—Apparatus for the preparation of culture media, e.g. bottling devices
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/30—Accessories for use before inoculation of spawn, e.g. sterilisers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/70—Harvesting
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Abstract
The present invention relates to a kind of methods that reduction cultivation of agaricus bisporus trichoderma occurs and improves yield, include the following steps：1) preparation of Cultivar culture medium, 2) fermentation reactor system, 3) pack, 4) it sterilizes, 5) Cultivar culture medium inoculation and culture, 6) preparation of mushroom producing culture material, 7) tunnel fermentation, 8) secondary fermentation, 9) sow, 10) bacterium germination, 11) earthing, 12) flower bud, 13) it harvests and 14) change of tide management.The present invention is easy to operate, quick, convenient for promoting, can reduce the problem of trichoderma occurs.
Description
Technical field
The present invention relates to reduce edible fungus culturing trichoderma to occur and improve the technology of yield more particularly to a kind of reduction pair spores
The method that mushroom-cultivating trichoderma occurs and improves yield.
Background technology
Agaricus bisporus be in the world artificial cultivation most extensively, yield highest, the maximum edible mushroom of consumption figure, have it is important
Economic value.China is since the 1930s introduces cultivation, and popularizing planting, wherein cultivated area are more throughout the country
Have the provinces such as Fujian, Shandong, Henan and Zhejiang, full of nutrition since agaricus bisporus bacterial context is plump, domestic consumption and outlet need
Amount is big.For artificial cultivation generally using in the full granular grain grown on matrix such as wheat, paddy, millet, strain pollution rate is high, broadcasts
Trichoderma infection easily occurs after kind；After harvesting 1 tide under the conditions of batch production, earthing surface will appear a large amount of trichodermas, lead to mushroom grower
The underproduction reduction of income causes the production of batch production agaricus bisporus to be very limited due to cultivation yield and unstable quality.Therefore it is
The cultivation amount for improving agaricus bisporus adapts to the needs of industrialized cultivation and improves biological efficiency, subtracts to agaricus bisporus development
The research for the technology that few edible fungus culturing trichoderma occurs and improves yield has great importance.
Reduce cultivation of agaricus bisporus trichoderma occur and improve yield key be Spawn incubation based formulas, plant formulation and
Preparation method, there are many ways to obtaining Spawn incubation based formulas, for example (,) it is wheat culture medium prescription, paddy culture medium prescription, small
Rice culture medium prescription and combined method.Specifically the technological process of production is：Stock → → packed → sterilizing of culture medium preparation →
Cooling → inoculation → culture.Relevant technology there are many patent.For example, number CN104823712A entitled " one
The anti-aging agaricus bisporus kernel culture and preparation method thereof of kind ", which is disclosed, to be made of in raw material barley or wheat, excrement and river silt
It is cultivated, the method for preparing strain.Number CN103387471A discloses the method using the cultivating bisporous mushroom of cornstalk.
But this cultural method the problem is that：Strain pollution rate is high, and trichoderma infection after planting easily occurs；First
Damp biological efficiency is relatively low, cultivation yield and unstable quality.Cultivation of agaricus bisporus trichoderma is preferably reduced therefore, it is necessary to a kind of
The method for occurring and improving yield.
Invention content
It is insufficient present on purpose of the present invention is to overcome, propose that a kind of reduction cultivation of agaricus bisporus trichoderma occurs and improves
The method of yield.
The object of the invention is achieved through the following technical solutions：
A method of it reducing cultivation of agaricus bisporus trichoderma and occurs and improve yield, include the following steps：
1) preparation of Cultivar culture medium：By cotton seed hulls 31%~43%, flour 3%~15%, urea 1%, lime
0.5%, gypsum 0.5%, water 52%, mixing, adapted；
2) fermentation reactor system：By step 1) Cultivar culture medium fermentation reactor system, heap rake is opened after 6d, is shaken up, and is kept the skin wet,
Again fermentation reactor system；Turning after 4d, and keeping the skin wet, again fermentation reactor system；Heap rake is opened after 3d, is shaken up, it is spare；
3) it packs：It is polypropylene of the 17cm × 36cm × 0.005cm to dog-ear that step 2) fermentation medium, which is packed into specification,
In polybag to bag it is high 2/3rds at, then will be after this bag close up, put on Plastibell and upper shield, adapted；
4) it sterilizes：Step 3) Cultivar culture medium is filled basket to sterilize into pot, using high pressure sterilization, first drains cold air in pot,
Steam pressure rises to 0.14MPa (123~126 DEG C of temperature) and keeps 2~3h；
5) Cultivar culture medium inoculation and culture：Step 4) Cultivar culture medium is taken out, moves into transfer room after cooling, presses
It is inoculated with according to sterile working, accesses agaricus bisporus seed, bacterium germination room is put into after inoculation, room temperature is set as 23 DEG C or so, and air is wet
Degree 60%, dark culturing, after cultivating 30d, as cultivation of agaricus bisporus kind；
6) preparation of mushroom producing culture material：Cross the corncob 15%~20% of 4 mesh sieve, the straw 11% of length 5cm~10cm
~16%, megasse 1.5%, rape cake 1%, urea 0.5%, lime 0.5%, gypsum 0.5%, water 65%, mixing, adapted；
7) tunnel ferments：Coordinate throwing machine to be sent into tunnel with charger in step 6) mushroom producing culture material and carry out one time fermentation,
So that material temperature is reached and is kept for 70~80 DEG C.The roll-over time distinguishes 4d, 8d, 11d, and corotation changes 3 times.After third time roll-over
14d removes compost；
8) secondary fermentation：After step 7) compost warehouse entry, increases wind speed and balanced by material temperature, after material temperature reaches 45~48 DEG C
Maintain 6h；After material layer temperature stable and consistent, by the way that material temperature to be gradually warming up to 58~60 DEG C to rise 1 DEG C of speed per hour, protect
Hold 8h；After pasteurization, material temperature is dropped to 48 DEG C or so in 10~12h, air quantity is adjusted by frequency converter, is followed in progress
Ring, adjusting breather valve makes compost temperature maintain 4~5d at 48~52 DEG C.Secondary fermentation about take 6~7d, after be
Material culture of agaricus bisporus；
9) it sows：Step 5) cultivation of agaricus bisporus kind is inoculated into step 8) compost, based on compost weight, with kind
Amount is 0.5%~0.7%；
10) bacterium germination：By the compost upper cover thin film of step 9), temperature maintains 20~25 DEG C, and material temperature maintains 24
~26 DEG C, mushroom house relative humidity should maintain 90% or so, 13~15d of bacterium germination time；
11) earthing：By earthing on step 10) bacterium germination compost, thickness is 4~4.5cm, and temperature maintains 23~25 DEG C,
Material temperature maintains 25~27 DEG C, keeps 10~13d；
12) flower bud：Material temperature is fifty-fifty down to 20 DEG C with daily 1 DEG C of speed, and temperature is down to 18 DEG C or so, increases fresh sky
Gas is reduced per angel's mushroom house CO2 equalization of concentration, is finally down to 1000ppm or so, sprays fruiting water, keeps 6~7d；
13) it harvests：When fructification 2~4.5cm of diameter, complete, full, flexible, the non-parachute-opening of mushroom shape, not at thin skin mushroom
When, harvest in time, when picking, should wear cleaning gloves, and first to end pressure, then gently rotation is adopted down, avoid driving around small mushroom；It adopts
It is cut with clean cutter when plucking clump mushroom, the mushroom under adopting should be put into the plastic container with stomata；
14) change of tide management：Bed surface after fruiting is smooth, keeps environmental sanitation, the second later damp mushroom, third damp mushroom management
With the first damp management of producing mushroom, per tide in 4~6d, until terminating.
Preferably, the weight ratio of cotton seed hulls is 36%~41% in step 1), flour weight ratio is 5%~10%.
Preferably, the weight ratio for the corncob that 4 mesh of mistake in step 6) sieves is 15%~18%, the weight ratio of straw is
13%~16%.
Preferably, the weight ratio of each component of Cultivar culture medium is in step 1)：Cotton seed hulls 36%, flour 10%,
Urea 1%, lime 0.5%, gypsum 0.5%, water 52%.
Preferably, the weight ratio of each component of mushroom producing culture material is in step 6)：The corncob 15% of 4 mesh sieve is crossed, it is long
Spend the straw 16% of 10cm, megasse 1.5%, rape cake 1%, urea 0.5%, lime 0.5%, gypsum 0.5%, water 65%.
The beneficial effects of the invention are as follows：
1, easy to operate, quick, reduce the problem of trichoderma occurs；The present invention uses mixed culture kind, success ratio of inoculation
99% or more, and ordinary culture medium inoculation in most cases pollution rate up to 2%~8%；After planting trichoderma infection rate 2% with
Under, and wheat culture medium trichoderma infection up to 5% or more；Mixed culture kind highest uses 15% wheat, and wheat culture medium
Using at least 40% wheat, grain is greatly saved, it is practical easily to promote.
2, the present invention uses corncob straw mushroom producing culture material, improves the biological efficiency of the first damp mushroom, up to 20%
More than, Nostoc commune Vanch material is 10%~15% or so, while efficiently using corncob, and economic benefit, ecological benefits are notable.
3, the present invention is occurred by reducing cultivation of agaricus bisporus trichoderma, can make full use of infrastructure, is quickly obtained a large amount of
Agaricus bisporus fructification is suitble to be factory produced, can produce 4~6 batches of agaricus bisporus every year, while also being adapted for planting under natural conditions
Training.
Specific implementation mode
By following embodiment, the present invention is described in further detail, but present disclosure is not limited to
This.
Embodiment 1
1) preparation of Cultivar culture medium：Under natural climate condition, 7~September Cultivar culture medium.Cotton seed hulls
41%, flour 5%, urea 1%, lime 0.5%, gypsum 0.5%, water 52%, mixing, adapted.
2) fermentation reactor system：By step 1) Cultivar culture medium fermentation reactor system, heap rake is opened after 6d, is shaken up, and is kept the skin wet,
Again fermentation reactor system；Turning after 4d, and keeping the skin wet, again fermentation reactor system；Heap rake is opened after 3d, is shaken up, it is spare.
3) it packs：It is polypropylene of the 17cm × 36cm × 0.005cm to dog-ear that step 2) fermentation medium, which is packed into specification,
In polybag to bag it is high 2/3rds at, then will be after this bag close up, put on Plastibell and upper shield, adapted.
4) it sterilizes：Step 3) Cultivar culture medium is filled basket to sterilize into pot, using high pressure sterilization, first drains cold air in pot,
Steam pressure rises to 0.14MPa (123~126 DEG C of temperature) and keeps 2~3h.
5) Cultivar culture medium inoculation and culture：Step 4) Cultivar culture medium is taken out, moves into transfer room after cooling, presses
It is inoculated with according to sterile working.Agaricus bisporus seed is accessed, bacterium germination room is put into after inoculation, room temperature is set as 23 DEG C or so, and air is wet
Degree 60%, dark culturing.After cultivating 30d, as cultivation of agaricus bisporus kind.
6) preparation of mushroom producing culture material：Cross the corncob 15% of 4 mesh sieve, the straw 16% of length 10cm, megasse
1.5%, rape cake 1%, urea 0.5%, lime 0.5%, gypsum 0.5%, water 65%, mixing, adapted.
7) tunnel ferments：Step 6) mushroom producing culture material is subjected to one time fermentation, so that material temperature is reached and is kept for 70~80 DEG C.It turns over
The heap time distinguishes 4d, 8d, 11d, and corotation changes 3 times.14d removes compost after third time turning.
8) secondary fermentation：After step 7) compost warehouse entry, increases wind speed and balanced by material temperature, after material temperature reaches 45~48 DEG C
Maintain 6h；After material layer temperature stable and consistent, by the way that material temperature to be gradually warming up to 58~60 DEG C to rise 1 DEG C of speed per hour, protect
Hold 8h；After pasteurization, material temperature is dropped to 48 DEG C or so in 10~12h, make compost temperature maintain 4 at 48~52 DEG C~
5d.Secondary fermentation about take 6~7d, after be material culture of agaricus bisporus.
9) it sows：Step 5) cultivation of agaricus bisporus kind is inoculated into step 8) compost, based on compost weight, with kind
Amount is 0.5%~0.7%.
10) bacterium germination：In bed surface lid thin film when step 9) is cultivated bacterium germination, temperature maintains 20~25 DEG C, material temperature dimension
It holds at 24~26 DEG C, mushroom house relative humidity should maintain 90% or so, 13~15d of bacterium germination time.
11) earthing：By earthing on step 10) bacterium germination compost, thickness is 4~4.5cm, and temperature maintains 23~25 DEG C,
Material temperature maintains 25~27 DEG C, keeps 10~13d.
12) flower bud：Material temperature is fifty-fifty down to 20 DEG C with daily 1 DEG C of speed, and temperature is down to 18 DEG C or so, increases fresh sky
Gas, per angel's mushroom house CO2Equalization of concentration reduces, and is finally down to 1000ppm or so, sprays fruiting water, keeps 6~7d.
13) it harvests：When fructification 2~4.5cm of diameter, complete, full, flexible, the non-parachute-opening of mushroom shape, not at thin skin mushroom
When, it harvests in time.Cleaning gloves should be worn when picking, first to end pressure, then gently rotation is adopted down, avoids driving the small mushroom of surrounding；It adopts
It is cut with clean cutter when plucking clump mushroom.Mushroom under adopting should be put into the plastic container with stomata.
14) change of tide management：Bed surface after fruiting is smooth, keeps environmental sanitation.Later the second damp mushroom, third damp mushroom management
With the first damp management of producing mushroom, per tide in 4~6d, until terminating.
Embodiment 2
1) preparation of Cultivar culture medium：Industrializing facility production can need Cultivar culture medium processed according to the anniversary.Cottonseed
Shell 36%, flour 10%, urea 1%, lime 0.5%, gypsum 0.5%, water 52%, mixing, adapted.
2) fermentation reactor system：By step 1) Cultivar culture medium fermentation reactor system, heap rake is opened after 6d, is shaken up, and is kept the skin wet,
Again fermentation reactor system；Turning after 4d, and keeping the skin wet, again fermentation reactor system；Heap rake is opened after 3d, is shaken up, it is spare.
3) it packs：It is polypropylene of the 17cm × 36cm × 0.005cm to dog-ear that step 2) fermentation medium, which is packed into specification,
In polybag to bag it is high 2/3rds at, then will be after this bag close up, put on Plastibell and upper shield, adapted.
4) it sterilizes：Step 3) Cultivar culture medium is filled basket to sterilize into pot, using high pressure sterilization, first drains cold air in pot,
Steam pressure rises to 0.14MPa (123~126 DEG C of temperature) and keeps 2~3h.
5) Cultivar culture medium inoculation and culture：Step 4) Cultivar culture medium is taken out, moves into transfer room after cooling, presses
It is inoculated with according to sterile working.Agaricus bisporus seed is accessed, bacterium germination room is put into after inoculation, room temperature is set as 23 DEG C or so, and air is wet
Degree 60%, dark culturing.After cultivating 30d, as cultivation of agaricus bisporus kind.
6) preparation of mushroom producing culture material：Industrializing facility production can need to make mushroom compost according to the anniversary.Cross 4 mesh sieve
Corncob 18%, the straw 13% of length 5cm, megasse 1.5%, rape cake 1%, urea 0.5%, lime 0.5%, gypsum
0.5%, water 65%, mixing, adapted.
7) tunnel ferments：Coordinate throwing machine to be sent into tunnel with charger in step 6) mushroom producing culture material and carry out one time fermentation,
So that material temperature is reached and is kept for 70~80 DEG C.The roll-over time distinguishes 4d, 8d, 11d, and corotation changes 3 times.After third time roll-over
14d removes compost.
8) secondary fermentation：After step 7) compost warehouse entry, increases wind speed and balanced by material temperature, after material temperature reaches 45~48 DEG C
Maintain 6h；After material layer temperature stable and consistent, by the way that material temperature to be gradually warming up to 58~60 DEG C to rise 1 DEG C of speed per hour, protect
Hold 8h；After pasteurization, material temperature is dropped to 48 DEG C or so in 10~12h, air quantity is adjusted by frequency converter, is followed in progress
Ring, adjusting breather valve makes compost temperature maintain 4~5d at 48~52 DEG C.Secondary fermentation about take 6~7d, after be
Material culture of agaricus bisporus.
9) it sows：Step 5) cultivation of agaricus bisporus kind is inoculated into step 8) compost, based on compost weight, with kind
Amount is 0.5%~0.7%.
10) bacterium germination：In bed surface lid thin film when step 9) is cultivated bacterium germination, temperature maintains 20~25 DEG C, material temperature dimension
It holds at 24~26 DEG C, mushroom house relative humidity should maintain 90% or so, 13~15d of bacterium germination time.
11) earthing：By earthing on step 10) bacterium germination compost, thickness is 4~4.5cm, and temperature maintains 23~25 DEG C,
Material temperature maintains 25~27 DEG C, keeps 10~13d.
12) flower bud：Material temperature is fifty-fifty down to 20 DEG C with daily 1 DEG C of speed, and temperature is down to 18 DEG C or so, increases fresh sky
Gas, per angel's mushroom house CO2Equalization of concentration reduces, and is finally down to 1000ppm or so, sprays fruiting water, keeps 6~7d.
13) it harvests：When fructification 2~4.5cm of diameter, complete, full, flexible, the non-parachute-opening of mushroom shape, not at thin skin mushroom
When, it harvests in time.Cleaning gloves should be worn when picking, first to end pressure, then gently rotation is adopted down, avoids driving the small mushroom of surrounding；It adopts
It is cut with clean cutter when plucking clump mushroom.Mushroom under adopting should be put into the plastic container with stomata.
14) change of tide management：Bed surface after fruiting is smooth, keeps environmental sanitation.Later the second damp mushroom, third damp mushroom management
With the first damp management of producing mushroom, per tide in 4~6d, until terminating.
Test example 3 (contrast test of the method for the present invention and conventional cultivation method)
Test site：Experiment is located at Zhejiang Academy of Agricultural Science edible mushroom test room
Test period：2016~2017 years
Experimental design：
1) two kinds of different each 500 bags of Cultivar culture mediums, are repeated 3 times；
2) two kinds of each 50m of different mushroom producing culture material2, it is repeated 3 times；
3) method of the present invention experiment with embodiment 2；
4) conventional cultivation method uses Cultivar culture medium formula：By cotton seed hulls 5%, wheat 42, lime 0.5%, gypsum
0.5%, water 52%, mixing is configured to Cultivar culture medium, packs high pressure sterilization.Mushroom producing culture material formula straw 30%, rapeseed cake
2%kg, urea 0.5%, ammonium sulfate 0.4%, calcium superphosphate 0.7%, gypsum 0.7%, lime 0.7%, water 65%.Remaining operation
It is identical as above-described embodiment 2.
5) the method for the present invention and the comparative test result of conventional cultivation method etc. are as shown in the table：
Claims (5)
1. a kind of method for reducing cultivation of agaricus bisporus trichoderma and occurring and improving yield, which is characterized in that include the following steps：
1) preparation of Cultivar culture medium：By cotton seed hulls 31%~43%, flour 3%~15%, urea 1%, lime 0.5%,
Gypsum 0.5%, water 52%, mixing, adapted；
2) fermentation reactor system：By step 1) Cultivar culture medium fermentation reactor system, heap rake is opened after 6d, is shaken up, and is kept the skin wet, again
Fermentation reactor system；Turning after 4d, and keeping the skin wet, again fermentation reactor system；Heap rake is opened after 3d, is shaken up, it is spare；
3) it packs：It is polypropylene plastics of the 17cm × 36cm × 0.005cm to dog-ear that step 2) fermentation medium, which is packed into specification,
Bag in bag it is high 2/3rds at, then will this bag close up, put on Plastibell and upper shield after, adapted；
4) it sterilizes：Step 3) Cultivar culture medium is filled basket to sterilize into pot, using high pressure sterilization, first drains cold air in pot, steam
Pressure rises to 0.14MPa (123~126 DEG C of temperature) and keeps 2~3h；
5) Cultivar culture medium inoculation and culture：Step 4) Cultivar culture medium is taken out, transfer room is moved into after cooling, according to nothing
Bacterium operation is inoculated with, and is accessed agaricus bisporus seed, bacterium germination room is put into after inoculation, room temperature is set as 23 DEG C or so, air humidity
60%, dark culturing, after cultivating 30d, as cultivation of agaricus bisporus kind；
6) preparation of mushroom producing culture material：Cross the corncob 15%~20% of 4 mesh sieve, the straw 11% of length 5cm~10cm~
16%, megasse 1.5%, rape cake 1%, urea 0.5%, lime 0.5%, gypsum 0.5%, water 65%, mixing, adapted；
7) tunnel ferments：Coordinate throwing machine to be sent into tunnel with charger in step 6) mushroom producing culture material to carry out one time fermentation, make material
Temperature reaches and is kept for 70~80 DEG C.The roll-over time distinguishes 4d, 8d, 11d, and corotation changes 3 times.14d after third time roll-over
Compost is removed；
8) secondary fermentation：After step 7) compost warehouse entry, increases wind speed and balanced by material temperature, material temperature maintains after reaching 45~48 DEG C
6h；After material layer temperature stable and consistent, by the way that material temperature to be gradually warming up to 58~60 DEG C to rise 1 DEG C of speed per hour, 8h is kept；
After pasteurization, material temperature is dropped to 48 DEG C or so in 10~12h, air quantity is adjusted by frequency converter, interior cycle is carried out, adjusts
Breather valve makes compost temperature maintain 4~5d at 48~52 DEG C.Secondary fermentation about takes 6~7d, after be double spore mushrooms
Mushroom compost；
9) it sows：Step 5) cultivation of agaricus bisporus kind is inoculated into step 8) compost, based on compost weight, sowing quantity is
0.5%~0.7%；
10) bacterium germination：By the compost upper cover thin film of step 9), temperature maintains 20~25 DEG C, and material temperature maintains 24~26
DEG C, mushroom house relative humidity should maintain 90% or so, 13~15d of bacterium germination time；
11) earthing：By earthing on step 10) bacterium germination compost, thickness is 4~4.5cm, and temperature maintains 23~25 DEG C, material temperature
25~27 DEG C are maintained, 10~13d is kept；
12) flower bud：Material temperature is fifty-fifty down to 20 DEG C with daily 1 DEG C of speed, and temperature is down to 18 DEG C or so, increases fresh air,
Per angel's mushroom house CO2Equalization of concentration reduces, and is finally down to 1000ppm or so, sprays fruiting water, keeps 6~7d；
13) it harvests：As fructification 2~4.5cm of diameter, complete, full, flexible, the non-parachute-opening of mushroom shape, not at thin skin mushroom when, and
Shi Caishou, when picking, should wear cleaning gloves, and first to end pressure, then gently rotation is adopted down, avoid driving around small mushroom；Pick clump mushroom
When cut with clean cutter, the mushroom under adopting should be put into the plastic container with stomata；
14) change of tide management：Bed surface after fruiting is smooth, keeps environmental sanitation, the second later damp mushroom, the management of third damp mushroom are the same as the
One damp management of producing mushroom, per tide in 4~6d, until terminating.
2. a kind of method for reducing cultivation of agaricus bisporus trichoderma and occurring and improving yield according to claim 1, feature
It is, the weight ratio of cotton seed hulls is 36%~41% in step 1), and flour weight ratio is 5%~10%.
3. a kind of method for reducing cultivation of agaricus bisporus trichoderma and occurring and improving yield according to claim 1, feature
It is, the weight ratio of the corncob of 4 mesh of the mistake sieve in step 6) is 15%~18%, and the weight ratio of straw is 13%~16%.
4. a kind of method for reducing cultivation of agaricus bisporus trichoderma and occurring and improving yield according to claim 1, feature
It is, the weight ratio of each component of Cultivar culture medium is in step 1)：Cotton seed hulls 36%, flour 10%, urea 1%, lime
0.5%, gypsum 0.5%, water 52%.
5. a kind of method for reducing cultivation of agaricus bisporus trichoderma and occurring and improving yield according to claim 1, feature
It is, the weight ratio of each component of mushroom producing culture material is in step 6)：Cross the corncob 15% of 4 mesh sieve, the straw of length 10cm
16%, megasse 1.5%, rape cake 1%, urea 0.5%, lime 0.5%, gypsum 0.5%, water 65%.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN110268918A (en) * | 2019-06-05 | 2019-09-24 | 凤台县香玉园食用菌种植专业合作社 | Culture medium is used in a kind of growth of agaricus bisporus |
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| CN111567320A (en) * | 2019-12-31 | 2020-08-25 | 浙江省农业科学院 | Agaricus bisporus Yingxiu culture medium capable of efficiently utilizing cotton stalks and preparation method and device thereof |
| CN111567320B (en) * | 2019-12-31 | 2023-09-19 | 浙江省农业科学院 | Agaricus bisporus Yingxiu medium capable of efficiently utilizing cotton stalk and preparation method and device thereof |
| CN111328633A (en) * | 2020-03-11 | 2020-06-26 | 杭州市农业科学研究院 | Agaricus bisporus casing material and preparation method thereof |
| CN111328633B (en) * | 2020-03-11 | 2022-06-21 | 杭州市农业科学研究院 | Agaricus bisporus casing material and preparation method thereof |
| CN111194664A (en) * | 2020-03-25 | 2020-05-26 | 浙江省农业科学院 | Agaricus bisporus culture medium and preparation method thereof |
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