CN108689782A - A kind of Rosa roxburghii Tratt special bio bacterial manure and the preparation method and application thereof - Google Patents
A kind of Rosa roxburghii Tratt special bio bacterial manure and the preparation method and application thereof Download PDFInfo
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- CN108689782A CN108689782A CN201810692787.0A CN201810692787A CN108689782A CN 108689782 A CN108689782 A CN 108689782A CN 201810692787 A CN201810692787 A CN 201810692787A CN 108689782 A CN108689782 A CN 108689782A
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- fertilizer
- rosa roxburghii
- roxburghii tratt
- bacterial manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of Rosa roxburghii Tratt special bio bacterial manure and the preparation method and application thereof,, with 10 parts of Bacillus cercus microbial inoculum, 5 parts of bacillus subtilis microbial agent, 5 parts of trichoderma bacteria agent, 30 parts of organic fertilizer, 10 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 15 parts of potash fertilizer, fertile 5 parts micro-, 2 parts of plant growth-promoting substance, 5 parts of humic acid, 5 parts of bentonite for raw material, Rosa roxburghii Tratt special bio bacterial manure is made in agitated mixing, screening, drying, packaging for it.Applied to yield, improvement Rosa roxburghii Tratt quality, raising Rosa roxburghii Tratt storage performance, induction enhancing Rosa roxburghii Tratt disease resistance, the growth for promoting Rosa roxburghii Tratt plant and raising the Rosa roxburghii Tratt root system micro organism quantity and soil enzyme activities etc. for increasing Rosa roxburghii Tratt.Have many advantages, such as high significant effect, green non-pollution, utilization rate of fertilizer, improvement soil, prevention and control disease, improve yield and quality, and is of low cost, easy to spread.
Description
Technical field
The present invention relates to Rosa roxburghii Tratt cultivation techniques and fertilising field, and in particular to a kind of Rosa roxburghii Tratt special bio bacterial manure and its preparation
Method and application.
Background technology
Rosa roxburghii Tratt (Rosaroxbunghii) is the fruit also known as mountain king fruit, thorn of the perennial machaka rosa roxburghii of the rose family
Certain kind of berries fruit, Fo Langguo, thatch pears, oblonga, alias thorn pineapple send the spring to return, pierce sour pear, nine-headed bird, Wen Xianguo, are healthcares
Nutrition treasure fruit is a kind of rare fruit.Pulp is crisp, has dense fragranced after ripe.Dense small corn, custom are referred to as on pericarp;
Ci Li ".Contain abundant vitamin C in fruit, is referred to as;The Wang " of Victoria C;.500 milligrams to 1000 milligrams of tannin, containing sour 1 gram of left side
The right side, general 4 grams or so of total sugar amount.It is born in endroit, cheuch, roadside and the bushes of 500 meters -2500 meters of height above sea level, is
The characteristic fine fruit and power-assisted in the Guizhou Province main force to assault fortified position that shakes off poverty get rich industry, are mainly distributed on Lupanshui City, Tongren
The industry centralizations such as city, Anshun, Zunyi development zone.
Currently, a large amount of uses, poor efficiency and its high environmental risk brought of chemical fertilizer are always to perplex in Rosa roxburghii Tratt production
The whole world significant problem, cause soil hardening, input increases, farmland pollution, agricultural product quality and safety decline etc. it is many after
Fruit has influenced the safety of Rosa roxburghii Tratt product and the sustainable development of Rosa roxburghii Tratt industry.And bio-bacterial manure is a kind of environmentally friendly
Fertilizer and one of the important directions of the future of agriculture sustainable development have and maintain and increase soil fertility, are conducive to micro- life
The breeding of object sociales, many advantages, such as keeping ecological balance, reduce environmental pollution.And there are no exclusively for thorn in the prior art
The ideal biological bacterial manure of pears design.
Invention content
The object of the invention is in view of the deficiencies of the prior art, it is desirable to provide a kind of green non-pollution, utilization rate of fertilizer be high, improvement
Soil, the Rosa roxburghii Tratt special bio bacterial manure for meeting Rosa roxburghii Tratt growing nutrient demand.
Technical scheme is as follows:
A kind of Rosa roxburghii Tratt special bio bacterial manure, it is made from the following raw materials in parts by weight:8-12 parts of Bacillus cercus microbial inoculum,
3-7 parts of bacillus subtilis microbial agent, 3-7 parts of trichoderma bacteria agent, 25-35 parts of organic fertilizer, 10-12 parts of nitrogenous fertilizer, 6-10 parts of phosphate fertilizer, potassium
12-18 parts fertile, micro- 3-7 parts of fertilizer, 1-3 parts of plant growth-promoting substance, 3-7 parts of humic acid, 3-7 parts of bentonite.
Preferably, it is made from the following raw materials in parts by weight:10 parts of Bacillus cercus microbial inoculum, bacillus subtilis microbial agent
5 parts, 5 parts of trichoderma bacteria agent, 30 parts of organic fertilizer, 10 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 15 parts of potash fertilizer, fertile 5 parts micro-, plant growth-promoting substance 2
Part, 5 parts of humic acid, 5 parts of bentonite.
Wherein, the strain contained in said waxy gemma bacillus agent is preferably Bacillus cercus LY05 (CCTCC NO:
M 2015397), Bacillus cercus LGY06 (CCTCC NO:M 2015398) and Bacillus cercus WL08 (CCTCCNO:
M2015399 a combination of one or more);The bacillus subtilis and Trichoderma are commercial product.Their making
Method is:After bacterial strain expansion connects cultivation and fermentation, bacterium solution is adsorbed with sterile solid absorbent, is placed on after mixing well quiet at dark
Set 2 days, then dried under the conditions of 25-30 DEG C, water tariff collection within 30% to get solid fungicide, living bacteria count >=
10000000000/gram.
Further, the solid absorbent is 3 by weight by maize straw powder+peanut shell powder+sawdust:2:1
It is made.
Further, organic fertilizer is by farm animal excrement, stalk, rubsen cake, peat soil, vinasse, Chinese medicine slag, edible fungi
Slag is 5 in mass ratio:2:1:1:0.5:0.5:0.5 uniformly mixing, and water tariff collection is added in 30-35%, pH 6.0-6.8
Enter the EM fermenting agents that mass percent is 2~3%, decomposed post-processing in 15-20 days is made.
Further, nitrogenous fertilizer refers to the group of one or more of ammonium fertilizer, nitrate fertilizer or amide nitrogen fertilizer
It closes;The phosphate fertilizer refers to the combination of one or more of phosphorus pentoxide, natural phosphate fertilizer or chemical phosphatic ferfilizer;The potash fertilizer
It refer to the combination of one or more of potassium chloride, potassium sulfate, plant ash, kainite;Micro- fertilizer for mass ratio is:2-
5:1-2:1-2:1-2:1-2:0.5-1:0.5-1:Calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer of 0.5-1
Mixed fertilizer;The plant growth-promoting substance is that mass ratio is:1-2:1-2::Chitosan+amino acid+seaweeds of 0.1-0.2;Institute
The humic acid water content stated is 3-5%, the content of organic matter >=75%.
The preparation method of the Rosa roxburghii Tratt special bio bacterial manure of the present invention, comprises the technical steps that:
Step 1. takes Bacillus cercus microbial inoculum, bacillus subtilis microbial agent, trichoderma bacteria agent, organic by regulation proportioning
Fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, micro- fertilizer, plant growth-promoting substance, humic acid, bentonite, it is spare;
The above raw material is placed in blender by step 2. to be thoroughly mixed uniformly;
For step 3. under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, the special life of Rosa roxburghii Tratt is made in screened, drying, packaging
Object bacterial manure.The bio-bacterial manure of the present invention can be applied at least one of following (1)-(3):
(1) increase the yield of Rosa roxburghii Tratt;(2) improve Rosa roxburghii Tratt quality, improve Rosa roxburghii Tratt storage performance;(3) induction enhancing Rosa roxburghii Tratt is anti-
Characteristic of disease, the diseases such as effective prevention and control Rosa roxburghii Tratt powdery mildew, brown spot, root rot, Sooty mould;(4) promote the growth of Rosa roxburghii Tratt plant;(5)
Improve Rosa roxburghii Tratt root system micro organism quantity and soil enzyme activities.
Compared with prior art, the present invention bio-bacterial manure of the present invention is using Bacillus cercus microbial inoculum, bacillus subtilis
Microbial inoculum, trichoderma bacteria agent, organic fertilizer, a great number of elements and trace element etc. form biologic organic, inorganic bacterial manure, can effectively improve thorn
Pears economic benefit, nutrient and flavour quality and storage performance promote plant well growth and improvement root soil environment, and can be effective
It induces Rosa roxburghii Tratt plant, the enhancing of fruit disease resistance and prevents the great diseases such as Rosa roxburghii Tratt powdery mildew, brown spot, root rot, Sooty mould
Occur, has high significant effect, green non-pollution, utilization rate of fertilizer, improvement soil, prevention and control disease, raising yield and quality etc. excellent
Point, and it is of low cost, easy to spread.
Specific implementation mode
With reference to embodiment, the invention will be further described, but not as any limitation of the invention.
Embodiment 1:
1. bio-bacterial manure is formed and is prepared:
Bio-bacterial manure A formula compositions and preparation, (1) composition:10 parts of Bacillus cercus microbial inoculum, bacillus subtilis microbial agent
5 parts, 5 parts of trichoderma bacteria agent, 30 parts of organic fertilizer, 10 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 15 parts of potash fertilizer, fertile 5 parts micro-, plant growth-promoting substance 2
Part, 5 parts of humic acid, 5 parts of bentonite.(2) it prepares:1. taking Bacillus cercus microbial inoculum, bacillus subtilis bacterium by regulation proportioning
Agent, trichoderma bacteria agent, organic fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, micro- fertilizer, plant growth-promoting substance, humic acid, bentonite, it is spare;2. walking
The rapid 1. above raw material is placed in blender is thoroughly mixed uniformly;3. in 25-30 DEG C of temperature, the condition of pH 6.0-6.8
Under, Rosa roxburghii Tratt special bio bacterial manure A is made in screened, drying, packaging.
Bio-bacterial manure B formula compositions and preparation, (1) composition:10 parts of bacillus subtilis microbial agent, 10 parts of trichoderma bacteria agent,
30 parts of organic fertilizer, 10 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 15 parts of potash fertilizer, fertile 5 parts micro-, 2 parts of plant growth-promoting substance, 5 parts of humic acid, bentonite
5 parts.(2) it prepares:1. by regulation proportioning take bacillus subtilis microbial agent, trichoderma bacteria agent, organic fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer,
Micro- fertilizer, plant growth-promoting substance, humic acid, bentonite, it is spare;2. step 1. the above raw material is placed in blender be sufficiently stirred it is mixed
It closes uniform;3. under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, Rosa roxburghii Tratt special bio bacterium is made in screened, drying, packaging
Fertile B.
2. field trial:
Indoor pot experiment designs:By Rosa roxburghii Tratt special bio bacterial manure for being applied on Rosa roxburghii Tratt, test in use, base manure is in shifting
It is applied when cultivation, accounts for total fertilizer 50%;Top dressing is used in mid-term, accounts for the 50% of fertilizer total amount.Made with conventional organic and inorganic fertilizer
For control, the same bio-bacterial manure of dose.Experiment sets 3 bio-bacterial manure A, bio-bacterial manure B, conventional fertilizer application processing, each processing 3 times
It repeats, often repeatedly 6 basin.
3. analysis project and method:
After fruit maturation, is harvested by cell, measure each cell single plant yield, 200 fruits of each cell random acquisition,
Take back experimental determination fruit quality, single fruit weight.Nutrient quality assay method:Dry matter is measured with National Standard Method method;Vitamin C contains
Amount is measured with 2,6 dichlorophenol indophenol method;Titratable acid (TA) content determination of acid-basetitration;Soluble solid (SSC) content
It is measured with PAL-1 type refractometers.Incidence and maturation history:Investigate Rosa roxburghii Tratt plant powdery mildew, brown spot, root rot, bituminous coal
A situation arises for disease.Incidence (%)=100 × (morbidity strain number/investigation strain number).
Soil root micro organism quantity and enzyme assay:Urease activity indophenols colorimetric method for determining, active unit is with 1g
Soil sample hydrolyzes the milligram number of the ammonia nitrogen of generation to indicate under the conditions of 37 DEG C after reaction in 24 hours;Sucrose activity phosphorus
Molybdic acid colorimetric method for determining, unit of enzyme activity is with (37 DEG C, 24 hours) meters of reduced sugar mg/g dry ground;Catalase is with using permanganic acid
Potassium titration, the ml that unit of enzyme activity reacts post consumption 0.1mol/LKMnO4 solution with 1g soil samples in 25 DEG C, 20min are come
It indicates;Alkaline phosphatase disodium phenyl phosphate colorimetric method for determining, unit of enzyme activity is with the 24 hours phenol (PhOH) generated of every gram of soil
Milligram number indicates.Edaphon clump count is measured using colony counting method:Bacterium beef-protein medium culture 24
It is counted after hour;Fungi is counted with PDA culture medium culture;Actinomyces are counted using Gause I synthetic media culture.
4. embodiment effect:
From table 1 to table 3 as can be seen that can effectively increase mean fruit weight, single plant using Rosa roxburghii Tratt special bio bacterial manure
Yield significantly improves fruit dry matter, vitamin C and soluble solid (SSC) content, reduces titratable acid (TA) content,
Promote the improvement of the formation and quality of Rosa roxburghii Tratt yield;And it can effectively reduce fruit of Grossularia burejensis Berger powdery mildew, brown spot, root rot, bituminous coal
Disease occurs;And soil enzyme activities can be improved and improve soil microbe quantity.
Influence of the application bio-bacterial manure of table 1 to Rosa roxburghii Tratt yield and quality
Note:With marking different lowercase letters after column data in P<0.05 time difference reaches the level of signifiance, different capitalizations
It indicates in P<0.01 time difference is same up to pole level of signifiance following tables
To Rosa roxburghii Tratt disease, a situation arises for the application of table 2 bio-bacterial manure
Influence of the application bio-bacterial manure of table 3 to Rosa roxburghii Tratt soil enzyme activities and micro organism quantity
The specific application example of the above only present invention, the present invention also has other embodiments, all to use equivalent replacement
Or the technical solution that equivalent transformation is formed, it all falls within protection domain of the presently claimed invention.
Claims (6)
1. a kind of Rosa roxburghii Tratt special bio bacterial manure, it is characterised in that:It is made from the following raw materials in parts by weight:Bacillus cercus bacterium
8-12 parts of agent, 3-7 parts of bacillus subtilis microbial agent, 3-7 parts of trichoderma bacteria agent, 25-35 parts of organic fertilizer, 10-12 parts of nitrogenous fertilizer, phosphate fertilizer
6-10 parts, 12-18 parts of potash fertilizer, micro- 3-7 parts of fertilizer, 1-3 parts of plant growth-promoting substance, 3-7 parts of humic acid, 3-7 parts of bentonite.
2. Rosa roxburghii Tratt special bio bacterial manure according to claim 1, it is characterised in that:It by following parts by weight raw material system
At:10 parts of Bacillus cercus microbial inoculum, 5 parts of bacillus subtilis microbial agent, 5 parts of trichoderma bacteria agent, 30 parts of organic fertilizer, nitrogenous fertilizer 10
Part, 8 parts of phosphate fertilizer, 15 parts of potash fertilizer, fertile 5 parts micro-, 2 parts of plant growth-promoting substance, 5 parts of humic acid, 5 parts of bentonite.
3. Rosa roxburghii Tratt special bio bacterial manure according to claim 1 or 2, it is characterised in that:The organic fertilizer is to arrange livestock and poultry
It is 5 to let out object, stalk, rubsen cake, peat soil, vinasse, Chinese medicine slag, edible fungi residue in mass ratio:2:1:1:0.5:0.5:0.5
Even mixing, and the EM fermenting agents that mass percent is 2~3% are added in 30-35%, pH 6.0-6.8 in water tariff collection,
Decomposed post-processing in 15-20 days is made.
4. Rosa roxburghii Tratt special bio bacterial manure according to claim 1 or 2, it is characterised in that:The nitrogenous fertilizer refer to ammonium fertilizer,
The combination of one or more of nitrate fertilizer or amide nitrogen fertilizer;The phosphate fertilizer refers to phosphorus pentoxide, natural phosphate fertilizer
Or the combination of one or more of chemical phosphatic ferfilizer;The potash fertilizer refers in potassium chloride, potassium sulfate, plant ash, kainite
A combination of one or more;Micro- fertilizer for mass ratio is:2-5:1-2:1-2:1-2:1-2:0.5-1:0.5-1:
The mixed fertilizer of calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer of 0.5-1;The plant growth-promoting substance is quality
Than for:1-2:1-2::Chitosan+amino acid+seaweeds of 0.1-0.2;The humic acid water content is 3-5%, organic matter
Content >=75%.
5. a kind of preparation method of Rosa roxburghii Tratt special bio bacterial manure as claimed in claim 1 or 2, it is characterised in that:Including as follows
Processing step:
Step 1. takes Bacillus cercus microbial inoculum, bacillus subtilis microbial agent, trichoderma bacteria agent, organic fertilizer, nitrogen by regulation proportioning
Fertilizer, phosphate fertilizer, potash fertilizer, micro- fertilizer, plant growth-promoting substance, humic acid, bentonite, it is spare;
The above raw material is placed in blender by step 2. to be thoroughly mixed uniformly;
For step 3. under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, Rosa roxburghii Tratt special bio bacterium is made in screened, drying, packaging
Fertilizer.
6. a kind of application of Rosa roxburghii Tratt special bio bacterial manure as claimed in claim 1 or 2, it is characterised in that including following side
The application in face:(1) increase the yield of Rosa roxburghii Tratt;(2) improve Rosa roxburghii Tratt quality, improve Rosa roxburghii Tratt storage performance;(3) induction enhancing Rosa roxburghii Tratt is anti-
Characteristic of disease, disease of effective prevention and control including Rosa roxburghii Tratt powdery mildew, brown spot, root rot, Sooty mould;(4) promote Rosa roxburghii Tratt plant
Growth;(5) Rosa roxburghii Tratt root system micro organism quantity and soil enzyme activities are improved.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810692787.0A CN108689782A (en) | 2018-06-29 | 2018-06-29 | A kind of Rosa roxburghii Tratt special bio bacterial manure and the preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810692787.0A CN108689782A (en) | 2018-06-29 | 2018-06-29 | A kind of Rosa roxburghii Tratt special bio bacterial manure and the preparation method and application thereof |
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| Publication Number | Publication Date |
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| CN108689782A true CN108689782A (en) | 2018-10-23 |
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| CN201810692787.0A Withdrawn CN108689782A (en) | 2018-06-29 | 2018-06-29 | A kind of Rosa roxburghii Tratt special bio bacterial manure and the preparation method and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110256125A (en) * | 2019-07-12 | 2019-09-20 | 诸城梅香园农业发展有限公司 | A kind of preparation method of composite biological fertilizer |
| CN110452038A (en) * | 2019-09-09 | 2019-11-15 | 郑强 | A kind of Chinese prickly ash bio-organic fertilizer special material and preparation method thereof |
-
2018
- 2018-06-29 CN CN201810692787.0A patent/CN108689782A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110256125A (en) * | 2019-07-12 | 2019-09-20 | 诸城梅香园农业发展有限公司 | A kind of preparation method of composite biological fertilizer |
| CN110452038A (en) * | 2019-09-09 | 2019-11-15 | 郑强 | A kind of Chinese prickly ash bio-organic fertilizer special material and preparation method thereof |
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Application publication date: 20181023 |