CN108484338A - A kind of grape special bio bacterial manure and the preparation method and application thereof - Google Patents
A kind of grape special bio bacterial manure and the preparation method and application thereof Download PDFInfo
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- CN108484338A CN108484338A CN201810692749.5A CN201810692749A CN108484338A CN 108484338 A CN108484338 A CN 108484338A CN 201810692749 A CN201810692749 A CN 201810692749A CN 108484338 A CN108484338 A CN 108484338A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of grape special bio bacterial manure and the preparation method and application thereof,, with 10 parts of Bacillus cercus microbial inoculum, 5 parts of bacillus subtilis microbial agent, 5 parts of trichoderma bacteria agent, 30 parts of organic fertilizer, 12 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 15 parts of potash fertilizer, fertile 5 parts micro-, 2 parts of plant growth-promoting substance, 4 parts of humic acid, 4 parts of bentonite for raw material, grape special bio bacterial manure is made in agitated mixing, screening, drying, packaging for it.Applied to yield, improvement grape quality, raising Grape During Storage performance, induction enhancing Disease Resistance of Grapevine, the growth for promoting grapevine seedling and raising the grape root system micro organism quantity and soil enzyme activities etc. for increasing grape.Have many advantages, such as high significant effect, green non-pollution, utilization rate of fertilizer, improvement soil, prevention and control disease, improve yield and quality, and is of low cost, easy to spread.
Description
Technical field
The present invention relates to vinegrowing technologies and fertilising field, and in particular to a kind of grape special bio bacterial manure and its preparation
Method and application.
Background technology
Grape is famous fruit, is eaten raw or raisins processed, and is made wine, and the wine foot after wine brewing can carry food and drink acid, root and rattan medicine
With energy preventing or arresting vomiting, tocolysis.Grape is the industry melted ecological benefits, economic benefit and social benefit and be integrated, the production of Development in Guizhou grape
Industry has advantageous resources advantage, is the important breakthrough mouth transformed agricultural production.In recent years, grape industry in Guizhou obtains
To very fast development, the ground such as In Qiannan, Qiandongnan Prefecture of Guizhou Province, Zunyi and Tongren City are mainly distributed, such as In Qiannan Sui Autonomous County of Sandu
" township of Chinese crystal grape " title is authorized by grape branch of Chinese Association of Agricultural Science Societies." three all grape ", " Acer palmatum ' Atropurpureum' grape " and " red rock Portugal
The brands such as grape " be even more it is favored by consumers and enjoy great prestige both at home and abroad, Guizhou grape industry has gone on the development road of " Brand Agriculture "
Road, Guizhou grape -growing areas is more than 500,000 mu at present.
Currently, a large amount of uses, poor efficiency and its high environmental risk brought of chemical fertilizer are always to perplex in grape production
The whole world significant problem, cause soil hardening, input increases, farmland pollution, agricultural product quality and safety decline etc. it is many after
Fruit has influenced the safety of grape product and the sustainable development of grape industry.And bio-bacterial manure is a kind of environmentally friendly
Fertilizer and one of the important directions of the future of agriculture sustainable development have and maintain and increase soil fertility, are conducive to micro- life
The breeding of object sociales, many advantages, such as keeping ecological balance, reduce environmental pollution.And there are no exclusively for Portugal in the prior art
The ideal biological bacterial manure of grape design.
Invention content
The object of the invention is in view of the deficiencies of the prior art, it is desirable to provide a kind of green non-pollution, utilization rate of fertilizer be high, improvement
Soil, the grape special bio bacterial manure for meeting grape growth nutritional need.
Technical scheme is as follows:
A kind of grape special bio bacterial manure, it is made from the following raw materials in parts by weight:8-12 parts of Bacillus cercus microbial inoculum,
3-7 parts of bacillus subtilis microbial agent, 3-7 parts of trichoderma bacteria agent, 25-35 parts of organic fertilizer, 10-15 parts of nitrogenous fertilizer, 6-10 parts of phosphate fertilizer, potassium
12-18 parts fertile, micro- 3-7 parts of fertilizer, 1-3 parts of plant growth-promoting substance, 2-6 parts of humic acid, 2-6 parts of bentonite.
Preferably, it is made from the following raw materials in parts by weight:10 parts of Bacillus cercus microbial inoculum, bacillus subtilis microbial agent
5 parts, 5 parts of trichoderma bacteria agent, 30 parts of organic fertilizer, 12 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 15 parts of potash fertilizer, fertile 5 parts micro-, plant growth-promoting substance 2
Part, 4 parts of humic acid, 4 parts of bentonite.
Wherein, the strain contained in said waxy gemma bacillus agent is preferably Bacillus cercus LY05 (CCTCC NO:
M 2015397), Bacillus cercus LGY06 (CCTCC NO:M 2015398) and Bacillus cercus WL08 (CCTCCNO:
M2015399 a combination of one or more);The bacillus subtilis and Trichoderma are commercial product.Their making
Method is:After bacterial strain expansion connects cultivation and fermentation, bacterium solution is adsorbed with sterile solid absorbent, is placed on after mixing well quiet at dark
Set 2 days, then dried under the conditions of 25-30 DEG C, water tariff collection within 30% to get solid fungicide, living bacteria count >=
10000000000/gram.
Further, the solid absorbent is 3 by weight by maize straw powder+peanut shell powder+sawdust:2:1
It is made.
Further, organic fertilizer is by farm animal excrement, stalk, rubsen cake, peat soil, vinasse, Chinese medicine slag, edible fungi
Slag is 5 in mass ratio:2:1:1:0.5:0.5:0.5 uniformly mixing, and water tariff collection is added in 30-35%, pH 6.0-6.8
Enter the EM fermenting agents that mass percent is 2-3%, decomposed post-processing in 15-20 days is made.
Further, nitrogenous fertilizer refers to the group of one or more of ammonium fertilizer, nitrate fertilizer or amide nitrogen fertilizer
It closes;Phosphate fertilizer refers to the combination of one or more of phosphorus pentoxide, natural phosphate fertilizer or chemical phosphatic ferfilizer;Potash fertilizer refers to chlorination
The combination of one or more of potassium, potassium sulfate, plant ash, kainite;Micro- fertilizer for mass ratio is:2-5:1-2:1-2:1-
2:1-2:0.5-1:0.5-1:The mixed fertilizer of calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer of 0.5-1;Plant
Growth-promoting substance is that mass ratio is:1-2:1-2:Chitosan+amino acid+seaweeds of 0.1-0.2;Humic acid water content is 3-5%,
The content of organic matter >=75%.
The preparation method of the grape special bio bacterial manure of the present invention, comprises the technical steps that:
Step 1. takes Bacillus cercus microbial inoculum, bacillus subtilis microbial agent, trichoderma bacteria agent, organic by regulation proportioning
Fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, micro- fertilizer, plant growth-promoting substance, humic acid, bentonite, it is spare;
The above raw material is placed in blender by step 2. to be thoroughly mixed uniformly;
For step 3. under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, the special life of grape is made in screened, drying, packaging
Object bacterial manure.
The bio-bacterial manure of the present invention can be applied in following (1)-(5) are at least one:
(1) increase the yield of grape;(2) improve grape quality, improve Grape During Storage performance;(3) induction enhancing grape is anti-
Characteristic of disease, the diseases such as effective prevention and control downy mildew of garpe, fruit rot, root rot, root knot, root-knot nematode;(4) promote low age and grow into forest
The growth of grapevine seedling;(5) grape root system micro organism quantity and soil enzyme activities are improved.
Compared with prior art, the present invention bio-bacterial manure of the present invention is using Bacillus cercus microbial inoculum, bacillus subtilis
Microbial inoculum, trichoderma bacteria agent, organic fertilizer, a great number of elements and trace element etc. form biologic organic, inorganic bacterial manure, can effectively improve Portugal
Grape economic benefit, nutrient and flavour quality and storage performance promote plant well growth and improvement root soil environment, and can be effective
Induction grapevine seedling, fruit disease resistance enhance and the weights such as prevention downy mildew of garpe, fruit rot, root rot, root knot, root-knot nematode
The generation of major disease has high significant effect, green non-pollution, utilization rate of fertilizer, improvement soil, prevention and control disease, improves yield
The advantages that quality, and it is of low cost, easy to spread.
Specific implementation mode
With reference to embodiment, the invention will be further described, but not as any limitation of the invention.
Embodiment 1:
1. bio-bacterial manure is formed and is prepared:
Bio-bacterial manure A formula compositions and preparation, (1) composition:10 parts of Bacillus cercus microbial inoculum, bacillus subtilis microbial agent
5 parts, 5 parts of trichoderma bacteria agent, 30 parts of organic fertilizer, 12 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 15 parts of potash fertilizer, fertile 5 parts micro-, plant growth-promoting substance 2
Part, 4 parts of humic acid, 4 parts of bentonite.(2) it prepares:1. taking Bacillus cercus microbial inoculum, bacillus subtilis bacterium by regulation proportioning
Agent, trichoderma bacteria agent, organic fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, micro- fertilizer, plant growth-promoting substance, humic acid, bentonite, it is spare;2. walking
The rapid 1. above raw material is placed in blender is thoroughly mixed uniformly;3. in 25-30 DEG C of temperature, the condition of pH 6.0-6.8
Under, grape special bio bacterial manure A is made in screened, drying, packaging.
Bio-bacterial manure B formula compositions and preparation, (1) composition:10 parts of bacillus subtilis microbial agent, 10 parts of trichoderma bacteria agent,
30 parts of organic fertilizer, 12 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 15 parts of potash fertilizer, fertile 5 parts micro-, 2 parts of plant growth-promoting substance, 4 parts of humic acid, bentonite
4 parts.(2) it prepares:1. by regulation proportioning take bacillus subtilis microbial agent, trichoderma bacteria agent, organic fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer,
Micro- fertilizer, plant growth-promoting substance, humic acid, bentonite, it is spare;2. step 1. the above raw material is placed in blender be sufficiently stirred it is mixed
It closes uniform;3. under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, grape special bio bacterium is made in screened, drying, packaging
Fertile B.
2. field trial:
Experimental field overview:Experimental field it is located at Guizhou Province Qiandongnan Prefecture of Guizhou Province Sansui county magistrate's Ji Xiang natural resources fine fruits Specialty Co-operative Organization
Vineyard, kind are " purple autumn grape ", and random in orchard before experiment, multiple spot mixing acquisition depth 0-60cm soil sample is as soil
Background value, pH value 5.66, total nitrogen content 1.48gkg-1, content of organic matter 32.54gkg-1, alkaline hydrolysis nitrogen content
For 98.65mgkg-1, available phosphorus content 54.25mgkg-1, effective potassium content is 53.34mgkg-1。
Field experimental design:By grape special bio bacterial manure be used for grape application, orchard in use, base manure in current year 10
It is month low to apply, account for total fertilizer 50%;Top dressing at the beginning of 3 months next years (account for fertilizer total amount 30%), (account for fertilizer total amount at the beginning of 4 months
10%) it, is applied at the beginning of 5 months (account for fertilizer total amount 10%), accounts for the 50% of fertilizer total amount.Using conventional organic and inorganic fertilizer as pair
According to the same bio-bacterial manure of dose..Experiment sets 3 bio-bacterial manure A, bio-bacterial manure B, conventional fertilizer application processing, each handles 3 weights
It is multiple.
3. analysis project and method:
After fruit maturation, is harvested by cell, measure each cell single plant yield, 200 fruits of each cell random acquisition,
Take back experimental determination fruit quality, single fruit weight.Nutrient quality assay method:Dry matter is measured with National Standard Method;Vitamin C content
It is measured with 2,6 dichlorophenol indophenol method;Titratable acid (TA) content determination of acid-basetitration;Soluble solid (SSC) content is used
PAL-1 type refractometers measure.Storage time is all rotted the time using institute's storaging grape.
Incidence and maturation history:It is every 2 days investigation fruit incidences to start softening in fruit, counts accumulative morbidity
Rate;And investigate grapevine seedling disease incidence.Incidence (%)=100 × (hair the number of sick fruits/investigation the total number of fruits) or incidence
(%)=100 × (morbidity strain number/investigation strain number).Growth of Grape situation, including strain are observed in fertilising in month next year 7-8
Height, the number of blade, maximum leaf length, maximum width of blade, the indexs such as stem girth, bud spacing at graft union.
Soil root micro organism quantity and enzyme assay:Urease activity indophenols colorimetric method for determining, active unit is with 1g
Soil sample hydrolyzes the milligram number of the ammonia nitrogen of generation to indicate under the conditions of 37 DEG C after reaction in 24 hours;Sucrose activity phosphorus
Molybdic acid colorimetric method for determining, unit of enzyme activity is with (37 DEG C, for 24 hours) meters of reduced sugar mg/g dry ground;Catalase is with using potassium permanganate
Titration, the ml that unit of enzyme activity reacts post consumption 0.1mol/L KMnO4 solution with 1g soil samples in 25 DEG C, 20min carry out table
Show;Alkaline phosphatase disodium phenyl phosphate colorimetric method for determining, phenol (PhOH) milli that unit of enzyme activity is generated for 24 hours with every gram of soil
Grams indicates.Edaphon clump count is measured using colony counting method:Bacterium is small with beef-protein medium culture 24
When after count;Fungi is counted with PDA culture medium culture;Actinomyces are counted using Gause I synthetic media culture.
4. embodiment effect:
From table 1 to table 4 as can be seen that using grape special bio bacterial manure can effectively per mu yield, significantly improve fruit
Dry matter, vitamin C and soluble solid (SSC) content reduce titratable acid (TA) content, promote the formation of vintage
With the improvement of quality;And it can effectively reduce grape fruit fruit rot, downy mildew, root rot, root knot, root knot nematode disease morbidity
Rate;The growth of grapevine seedling can be effectively facilitated, soil enzyme activities is improved and improves soil microbe quantity.
Influence of the application bio-bacterial manure of table 1 to vintage quality
Note:With marking different lowercase letters after column data in P<0.05 time difference reaches the level of signifiance, different capitalizations
It indicates in P<0.01 time difference is same up to pole level of signifiance following tables
To grape disease, a situation arises for the application of table 2 bio-bacterial manure
3 vineyard of table uses bio-bacterial manure plant growth condition
Influence of the application bio-bacterial manure of table 4 to grape soil enzyme activities and micro organism quantity
The specific application example of the above only present invention, the present invention also has other embodiments, all to use equivalent replacement
Or the technical solution that equivalent transformation is formed, it all falls within protection domain of the presently claimed invention.
Claims (6)
1. a kind of grape special bio bacterial manure, it is characterised in that:It is made from the following raw materials in parts by weight:Bacillus cercus bacterium
8-12 parts of agent, 3-7 parts of bacillus subtilis microbial agent, 3-7 parts of trichoderma bacteria agent, 25-35 parts of organic fertilizer, 10-15 parts of nitrogenous fertilizer, phosphate fertilizer
6-10 parts, 12-18 parts of potash fertilizer, micro- 3-7 parts of fertilizer, 1-3 parts of plant growth-promoting substance, 2-6 parts of humic acid, 2-6 parts of bentonite.
2. grape special bio bacterial manure according to claim 1, it is characterised in that:It by following parts by weight raw material system
At:10 parts of Bacillus cercus microbial inoculum, 5 parts of bacillus subtilis microbial agent, 5 parts of trichoderma bacteria agent, 30 parts of organic fertilizer, nitrogenous fertilizer 12
Part, 8 parts of phosphate fertilizer, 15 parts of potash fertilizer, fertile 5 parts micro-, 2 parts of plant growth-promoting substance, 4 parts of humic acid, 4 parts of bentonite.
3. grape special bio bacterial manure according to claim 1 or 2, it is characterised in that:The organic fertilizer is to arrange livestock and poultry
It is 5 to let out object, stalk, rubsen cake, peat soil, vinasse, Chinese medicine slag, edible fungi residue in mass ratio:2:1:1:0.5:0.5:0.5
Even mixing, and by water tariff collection in 30-35%, pH 6.0-6.8, the EM fermenting agents that mass percent is 2-3% are added, it is rotten
Ripe post-processing in 15-20 days is made.
4. grape special bio bacterial manure according to claim 1 or 2, it is characterised in that:The nitrogenous fertilizer refer to ammonium fertilizer,
The combination of one or more of nitrate fertilizer or amide nitrogen fertilizer;The phosphate fertilizer refers to phosphorus pentoxide, natural phosphate fertilizer
Or the combination of one or more of chemical phosphatic ferfilizer;The potash fertilizer refers in potassium chloride, potassium sulfate, plant ash, kainite
A combination of one or more;Micro- fertilizer for mass ratio is:2-5:1-2:1-2:1-2:1-2:0.5-1:0.5-1:
The mixed fertilizer of calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer of 0.5-1;The plant growth-promoting substance is quality
Than for:1-2:1-2::Chitosan+amino acid+seaweeds of 0.1-0.2;The humic acid water content is 3-5%, organic matter
Content >=75%.
5. a kind of preparation method of grape special bio bacterial manure as claimed in claim 1 or 2, it is characterised in that:Including as follows
Processing step:
Step 1. takes Bacillus cercus microbial inoculum, bacillus subtilis microbial agent, trichoderma bacteria agent, organic fertilizer, nitrogen by regulation proportioning
Fertilizer, phosphate fertilizer, potash fertilizer, micro- fertilizer, plant growth-promoting substance, humic acid, bentonite, it is spare;
The above raw material is placed in blender by step 2. to be thoroughly mixed uniformly;
For step 3. under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, grape special bio bacterium is made in screened, drying, packaging
Fertilizer.
6. a kind of application of grape special bio bacterial manure as claimed in claim 1 or 2, it is characterised in that including following side
The application in face:(1) increase the yield of grape;(2) improve grape quality, improve Grape During Storage performance;(3) induction enhancing grape is anti-
Characteristic of disease, disease of effective prevention and control including downy mildew of garpe, fruit rot, root rot, root knot, root-knot nematode;(4) promote low
The growth in age and the grapevine seedling that grows into forest;(5) grape root system micro organism quantity and soil enzyme activities are improved.
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108863619A (en) * | 2018-09-19 | 2018-11-23 | 贵州大学 | A kind of Rosa roxburghii Tratt selenium-rich liquid bacterial manure and the preparation method and application thereof |
CN108976047A (en) * | 2018-09-19 | 2018-12-11 | 贵州大学 | A kind of Kiwi berry selenium-rich liquid bacterial manure and the preparation method and application thereof |
CN109369242A (en) * | 2018-09-19 | 2019-02-22 | 贵州大学 | A kind of blueberry selenium-rich liquid bacterial manure and the preparation method and application thereof |
CN109438097A (en) * | 2018-12-29 | 2019-03-08 | 镇江贝思特有机活性肥料有限公司 | Dedicated complete first biological organic fertilizer of a kind of grape and preparation method thereof |
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CN111517878A (en) * | 2020-05-30 | 2020-08-11 | 博兴天竹菌剂生物科技有限公司 | Biological source bacterial fertilizer system special for grapes and using method thereof |
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2018
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CN108863619A (en) * | 2018-09-19 | 2018-11-23 | 贵州大学 | A kind of Rosa roxburghii Tratt selenium-rich liquid bacterial manure and the preparation method and application thereof |
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WO2020082302A1 (en) * | 2018-10-22 | 2020-04-30 | 黄国伦 | Compound fertilizer for root restriction cultivation of grapes and preparation thereof |
CN109438097A (en) * | 2018-12-29 | 2019-03-08 | 镇江贝思特有机活性肥料有限公司 | Dedicated complete first biological organic fertilizer of a kind of grape and preparation method thereof |
CN111517878A (en) * | 2020-05-30 | 2020-08-11 | 博兴天竹菌剂生物科技有限公司 | Biological source bacterial fertilizer system special for grapes and using method thereof |
CN111943775A (en) * | 2020-07-27 | 2020-11-17 | 六安市叶集区绿叶果木种植专业合作社 | Grape fertilizer with early-maturing effect and cultivation method thereof |
CN114195596A (en) * | 2021-12-16 | 2022-03-18 | 山西欣普旺农业科技有限公司 | Aroma-enhancing organic water-soluble fertilizer for sunshine grapes and preparation method thereof |
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