CN108684710A - A kind of growth-promoting preparation and preparation method preventing ginkgo stem rot - Google Patents
A kind of growth-promoting preparation and preparation method preventing ginkgo stem rot Download PDFInfo
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- CN108684710A CN108684710A CN201810452044.6A CN201810452044A CN108684710A CN 108684710 A CN108684710 A CN 108684710A CN 201810452044 A CN201810452044 A CN 201810452044A CN 108684710 A CN108684710 A CN 108684710A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The present invention relates to biotechnology, specifically a kind of growth-promoting preparation for preventing ginkgo stem rot is made of the raw material of following parts by weight:Pseudomonas chlororaphis is subspecies 1.5-3.5 parts orange, 5.6-7.8 parts of Methylotrophic bacillus, 15.0-20.0 parts of 4.3-6.5 parts of Pseudomonas fluorescens and distilled water.The invention further relates to a kind of preparation method and applications for the growth-promoting preparation preventing ginkgo stem rot.Growth-promoting preparation through the invention, it is not simple to prevent ginkgo stem rot, but ginkgo stem rot is prevented with the growth-promoting preparation of the present invention, incidence is set to be reduced to 10% or less from 40-70%, greatly improve the survival rate of Seedlings of Ginkgo biloba, planting cost is reduced, economic value is improved.
Description
Technical field
The present invention relates to biotechnology, specifically a kind of growth-promoting preparation for preventing ginkgo stem rot.
The invention further relates to a kind of preparation method and applications for the growth-promoting preparation preventing ginkgo stem rot.
Background technology
The pathogen of ginkgo stem rot is Sphaeropsidales, Sphaeropsidaceae, the charcoal rot bacterium that big stem point belongs to.This bacterium is pyrophilous,
Suitable growth temperature is 30~32 DEG C;And it is not tight to acid-base value requirement, it can be grown very well between pH4~9.Morbidity is just
Phase seedling base portion browning, blade loses normal green, and slightly lets droop, but does not fall off.Susceptible position extends rapidly upward, so that
Complete stool is withered.There is shrinkage in sick seedling base portion cortex, and intradermal tissue rots to be in spongy or powdered, and color is greyish white, and accompanies many
The sclerotium of tiny black.This sick germ can also invade seedling xylem, thus the hollow marrow of brown is also shown in that bulbil produces sometimes
It is raw.Hereafter germ is gradually extended to root, and root skin cortex is made to rot.Hand-lifting sick seedling is such as used, xylem can only be extracted, root cortex is then
It stays among soil.Under conditions of high temperature or low temperature, stem rot can also occur ginkgo cuttage seeding, and cuttings epidermis can be made to be cylindrical in shape
It is sleeved on xylem, the thin pendulum tissue of bast, which then all blacks, to rot.
Pathogen usually seeks saprogenesis in the soil, belongs to weak parasitical fungi.Under optimum conditions from nursery stock wound
Place's intrusion.Therefore, disease occurs with host and on the spot that environmental condition is related.The aggrieved basic reason of nursery stock is due to surface temperature
Excessively high, nursery stock base portion causes germ to invade after by burn.The incidence of this lower disease of nursery stock degree of lignification is higher.In seedling
When the low-lying ponding of bed, incidence also obviously increases.Ginkgo cuttage seeding is inserted in 6~August part when seedbed high temperature is up to 30 DEG C or more
10~l5d starts to fall ill afterwards, and large area scion nigrescence is dead when serious.
The reason of being fallen ill according to ginkgo stem rot, should aim at prevention now.A. sowing is striven for when soil thaws i.e. ahead of time
Row sowing, this measure are conducive to nursery stock early stage lignifying, enhance the resilience to native table high temperature.B. reasonable heavy seeding heavy seeding has
Conducive to the population effect of nursery stock is played, enhance the drag to extraneous poor environment.Experiment proves that the smaller incidence of stocking is cured
Height, the bigger morbidity thing of density are lower.Practice also confirms that the past sows 25~40kg seeds per acre, is such as changed to 80~l00kg per acre
After the application rate of seed, not only incidence reduces, but also unit production seedling amount increases, and had not only saved soil but also had reduced morbidity.
C. prevention subterranean pest-insect nursery stock is after by the harm of subterranean pest-insect;It is easily infected by Pathogen, therefore, is sowed front and back certain
The moment is wanted to pay attention to eliminating subterranean pest-insect.D. the mechanical damage current year raw tree seedling or annual nursery transplant for preventing nursery stock are removed loosening the soil
The rhizome for being careful not to damage nursery stock is had to during grass or lifting plant, otherwise easily causes the generation of stem rot.E. it hides
To prevent solar radiation ground temperature from increasing, nursery, which should be taken, takes cool canopy, in the ranks covers grass, maize planting, sunshade of transplanting etc. for shade cooling
Measure is to reduce the harm to seedling.F. water spray of pouring water should pour water water spray to reduce surface temperature in time in high temperature season, there is item
The ground of part can take spray to irrigate, and be more advantageous to the generation for reducing disease.
Experiment proves that Antagonism actinomyces can effectively inhibit the sprawling of the disease germ to spread.
Invention content
Technical problem to be solved by the present invention lies in overcome the deficiencies of the prior art and provide a kind of prevention ginkgo stem rot
The growth-promoting preparation of disease.
In order to solve the above technical problems, the present invention now proposes following technical scheme:A kind of growth-promoting for preventing ginkgo stem rot
Preparation is made of the raw material of following parts by weight:Pseudomonas chlororaphis is subspecies 1.5-3.5 parts orange, Methylotrophic bacillus
15.0-20.0 parts of 5.6-7.8 parts, 4.3-6.5 parts of Pseudomonas fluorescens and distilled water.
Further, it is made of the raw material of following parts by weight:2.5 parts of the orange subspecies of Pseudomonas chlororaphis, Methylotrophic
18 parts of 6.6 parts of bacillus, 5.4 parts of Pseudomonas fluorescens and distilled water.
Further, the cultural method of the orange subspecies of the Pseudomonas chlororaphis is:The streak inoculation on KB culture mediums, it is non-
Under anaerobic environment, colony diameter reaches 2mm after being cultivated at 28 DEG C for 24 hours, the round micro- protuberance of bacterium colony, yellowish, the smooth of the edge, surface compared with
Moistening, is easily provoked, and thalline generates Chinese red pigment after cultivating 48h, is covered in bacterium colony surface, while periphery of bacterial colonies generation is orange
Color pigment.
Further, the formula of the KB culture mediums is:Containing containing 20 grams of peptone, 15 milli of glycerine in every 1000 milliliters of water
It rises, K2HPO40.392 grams and 0.732 gram of MgSO4, pH 6.5-7.5.
Further, the cultural method of the Methylotrophic bacillus is:First Methylotrophic bacillus is existed
Culture to strain density OD600 values are 0.6-0.8 in seed culture medium, bacterium solution are obtained, then in 100 parts by weight inoculation of medium
The bacterium solution of the Methylotrophic bacillus of 2-5 parts by weight, 37 DEG C of cultures.
Further, the ingredient of the seed culture medium is:Tryptone 10g/L, yeast extract 5g/L and
NaCl10g/L;The ingredient of the 100 parts by weight culture medium is:Glucose 15g, starch 1g, beancake powder 25g, manganese sulfate 1g, phosphorus
Acid dihydride potassium 1.5g, magnesium sulfate 0.5g, yeast extract 0.2g, iron chloride 0.1g, calcium carbonate 0.1g, pH7.0-7.2.
Further, the cultural method of the Pseudomonas fluorescens is:The streak inoculation on KB culture mediums, non-anaerobic environment
Under, 48h is cultivated at 28 DEG C, the well-grown on KB culture mediums, bacterium colony is to swell, and faint yellow, the smooth of the edge, surface relatively moistens,
Easily provoke;Water-soluble yellow green pigment is generated, it is visible under 254nm ultraviolet lights to there is strong yellow-green fluorescence to generate,
3-4d is cultivated, water colo(u)r can diffuse in culture medium, and yellow-green fluorescence gradually weakens to disappearance.
Further, the formula of the KB culture mediums is:Containing containing 20 grams of peptone, 15 milli of glycerine in every 1000 milliliters of water
It rises, K2HPO40.392 grams and 0.732 gram of MgSO4, pH 6.5-7.5.
It is another object of the present invention to additionally provide a kind of preparation method for the growth-promoting preparation preventing ginkgo stem rot, including
Following steps:(1) the streak inoculation Pseudomonas chlororaphis on KB culture mediums under non-anaerobic environment, cultivates -48h for 24 hours at 28 DEG C;
(2) it is 0.6-0.8 to cultivate Methylotrophic bacillus in seed culture medium to strain density OD600 values, obtains bacterium solution, so
Afterwards in the bacterium solution of the Methylotrophic bacillus of 100 parts by weight inoculation of medium 2-5 parts by weight, 37 DEG C of cultures;(3) in KB
Streak inoculation Pseudomonas fluorescens on culture medium under non-anaerobic environment, cultivates 48h, under 254nm ultraviolet lights at 28 DEG C
It can be seen that there is strong yellow-green fluorescence to generate, 3-4d is cultivated;(4) it adds distilled water according to the ratio into container, seals at room temperature
For a period of time;(4) at room temperature, the orange subspecies of Pseudomonas chlororaphis, Methylotrophic bacillus are then added according to the ratio successively
And Pseudomonas fluorescens, concussion are uniform, you can.
Another object of the present invention is application of the growth-promoting preparation in promoting ginkgo leaf in lactone B content.
Compared with prior art, the beneficial effects of the present invention are:1, growth-promoting preparation through the invention is not simple
Ginkgo stem rot is prevented, but with the present invention growth-promoting preparation ginkgo stem rot is prevented, make incidence from
40-70% is reduced to 10% hereinafter, greatly improve the survival rate of Seedlings of Ginkgo biloba, reduces planting cost, improves economy
Value;
2, growth-promoting preparation production method of the invention is simple, highly practical.
Specific implementation mode
, there are further understanding and understanding in the effect of to make to structure feature of the invention and being reached, to preferable
Embodiment is described in detail, and is described as follows:
Embodiment 1
A kind of growth-promoting preparation preventing ginkgo stem rot, is made of the raw material of following parts by weight:Pseudomonas chlororaphis is orange
15.0 parts of 1.5 parts of subspecies, 5.6 parts of Methylotrophic bacillus, 4.3 parts of Pseudomonas fluorescens and distilled water.
Embodiment 2
A kind of growth-promoting preparation preventing ginkgo stem rot, is made of the raw material of following parts by weight:Pseudomonas chlororaphis is orange
18.0 parts of 2.5 parts of subspecies, 6.6 parts of Methylotrophic bacillus, 5.4 parts of Pseudomonas fluorescens and distilled water.
Embodiment 3
A kind of growth-promoting preparation preventing ginkgo stem rot, is made of the raw material of following parts by weight:Pseudomonas chlororaphis is orange
20.0 parts of 3.5 parts of subspecies, 7.8 parts of Methylotrophic bacillus, 6.5 parts of Pseudomonas fluorescens and distilled water.
Embodiment 4
A kind of preparation method for the growth-promoting preparation preventing ginkgo stem rot, includes the following steps:(1) it is drawn on KB culture mediums
Line is inoculated with Pseudomonas chlororaphis, and under non-anaerobic environment, -48h for 24 hours is cultivated at 28 DEG C;(2) Methylotrophic bacillus is being planted
Culture to strain density OD600 values are 0.6-0.8 in sub- culture medium, bacterium solution are obtained, then in 100 parts by weight inoculation of medium
The bacterium solution of the Methylotrophic bacillus of 2-5 parts by weight, 37 DEG C of cultures;(3) streak inoculation fluorescence is false single on KB culture mediums
Under non-anaerobic environment, 48h is cultivated at 28 DEG C for born of the same parents bacterium, visible under 254nm ultraviolet lights to have strong yellow-green fluorescence production
It is raw, cultivate 3-4d;(4) distilled water is added according to the ratio into container, at room temperature sealing a period of time;(4) at room temperature, then according to
Secondary to add the orange subspecies of Pseudomonas chlororaphis, Methylotrophic bacillus and Pseudomonas fluorescens according to the ratio, concussion is uniform, i.e.,
It can.
Further, in the present embodiment, the cultural method of the orange subspecies of Pseudomonas chlororaphis is:It crosses on KB culture mediums
It is inoculated with, under non-anaerobic environment, colony diameter reaches 2mm, the round micro- protuberance of bacterium colony, yellowish, edge-light after being cultivated at 28 DEG C for 24 hours
Sliding, surface relatively moistens, and easily provokes, and thalline generates Chinese red pigment after cultivating 48h, is covered in bacterium colony surface, while periphery of bacterial colonies
Generate crocus pigment;The formula of KB culture mediums is:In every 1000 milliliters of water containing containing 20 grams of peptone, 15 milliliters of glycerine,
K2HPO40.392 grams and 0.732 gram of MgSO4, pH 6.5-7.5.
Further, in the present embodiment, the cultural method of Methylotrophic bacillus is:First by Methylotrophic gemma
It is 0.6-0.8 that bacillus, which is cultivated in seed culture medium to strain density OD600 values, bacterium solution is obtained, then in 100 parts by weight culture mediums
The bacterium solution of the Methylotrophic bacillus of middle inoculation 2-5 parts by weight, 37 DEG C of cultures;The ingredient of seed culture medium is:Tryptose
Peptone 10g/L, yeast extract 5g/L and NaCl10g/L;The ingredient of 100 parts by weight culture mediums is:Glucose 15g, starch 1g,
Beancake powder 25g, manganese sulfate 1g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.5g, yeast extract 0.2g, iron chloride 0.1g, calcium carbonate
0.1g, pH7.0-7.2.
Further, in the present embodiment, the cultural method of Pseudomonas fluorescens is:The streak inoculation on KB culture mediums, it is non-
Under anaerobic environment, 48h is cultivated at 28 DEG C, the well-grown on KB culture mediums, bacterium colony is protuberance, faint yellow, the smooth of the edge, surface
Compared with moistening, easily provoke;Water-soluble yellow green pigment is generated, it is visible under 254nm ultraviolet lights to have strong yellow green glimmering
Light generates, and cultivates 3-4d, and water colo(u)r can diffuse in culture medium, and yellow-green fluorescence, which gradually weakens, extremely to disappear;KB culture mediums
Formula be:Containing containing 20 grams of peptone, 15 milliliters, K2HPO40.392 grams of glycerine and MgSO4 0.732 in every 1000 milliliters of water
Gram, pH 6.5-7.5.
Test example
By test example as can be seen that each ingredient of the growth-promoting preparation of the present invention has promotion to preventing ginkgo stem rot
Effect, but after combining the promotion preparation for forming the present invention, the incidence of Seedlings of Ginkgo biloba is lower, survival rate higher, effect
Fruit is more preferable, and can show that the proportioning facilitation of each ingredient of embodiment 2 is best, and the incidence of ginkgo stem rot is only
6%.
Finally it should be noted that:The above embodiments are merely illustrative of the technical solutions of the present invention, rather than to limit it;
Although the present invention is described in detail referring to the foregoing embodiments, it will be understood by those of skill in the art that it still may be used
To modify to the technical solution that previous embodiment is recorded or equivalent replacement of some of the technical features;And this
A little modifications or substitutions, the spirit and scope for various embodiments of the present invention technical solution that it does not separate the essence of the corresponding technical solution.
Claims (10)
1. a kind of growth-promoting preparation preventing ginkgo stem rot, which is characterized in that be made of the raw material of following parts by weight:Green needle is false single
Born of the same parents bacterium is subspecies 1.5-3.5 parts orange, 5.6-7.8 parts of Methylotrophic bacillus, 4.3-6.5 parts of Pseudomonas fluorescens and distillation
15.0-20.0 parts of water.
2. a kind of growth-promoting preparation preventing ginkgo stem rot according to claim 1, which is characterized in that by following parts by weight
Raw material composition:2.5 parts of the orange subspecies of Pseudomonas chlororaphis, 6.6 parts of Methylotrophic bacillus, Pseudomonas fluorescens 5.4
18 parts of part and distilled water.
3. a kind of growth-promoting preparation preventing ginkgo stem rot according to claim 1, which is characterized in that the green needle is false single
The cultural method of the orange subspecies of born of the same parents bacterium is:The streak inoculation on KB culture mediums, under non-anaerobic environment, bacterium after being cultivated at 28 DEG C for 24 hours
It falls diameter and reaches 2mm, the round micro- protuberance of bacterium colony, yellowish, the smooth of the edge, surface relatively moistens, easily provoke, and thalline produces after cultivating 48h
Raw Chinese red pigment is covered in bacterium colony surface, while periphery of bacterial colonies generates crocus pigment.
4. a kind of growth-promoting preparation preventing ginkgo stem rot according to claim 3, which is characterized in that the KB culture mediums
Formula be:Containing containing 20 grams of peptone, 15 milliliters, K2HPO40.392 grams of glycerine and MgSO4 0.732 in every 1000 milliliters of water
Gram, pH 6.5-7.5.
5. a kind of growth-promoting preparation preventing ginkgo stem rot according to claim 1, which is characterized in that the methylotrophy
The cultural method of type bacillus is:First Methylotrophic bacillus is cultivated in seed culture medium to strain density OD600
Value is 0.6-0.8, bacterium solution is obtained, then in the Methylotrophic bacillus of 100 parts by weight inoculation of medium 2-5 parts by weight
Bacterium solution, 37 DEG C culture.
6. a kind of growth-promoting preparation preventing ginkgo stem rot according to claim 5, which is characterized in that the seed culture
The ingredient of base is:Tryptone 10g/L, yeast extract 5g/L and NaCl10g/L;The ingredient of the 100 parts by weight culture medium
For:Glucose 15g, starch 1g, beancake powder 25g, manganese sulfate 1g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.5g, yeast extract 0.2g,
Iron chloride 0.1g, calcium carbonate 0.1g, pH7.0-7.2.
7. a kind of growth-promoting preparation preventing ginkgo stem rot according to claim 1, which is characterized in that the fluorescence is false single
The cultural method of born of the same parents bacterium is:The streak inoculation on KB culture mediums under non-anaerobic environment, cultivates 48h, on KB culture mediums at 28 DEG C
Well-grown, bacterium colony are protuberance, and faint yellow, the smooth of the edge, surface relatively moistens, easily provokes;Water-soluble yellow green pigment is generated,
It is visible under 254nm ultraviolet lights to there is strong yellow-green fluorescence to generate, 3-4d is cultivated, water colo(u)r can diffuse to culture
In base, yellow-green fluorescence gradually weakens to disappearance.
8. a kind of growth-promoting preparation preventing ginkgo stem rot according to claim 7, which is characterized in that the KB culture mediums
Formula be:Containing containing 20 grams of peptone, 15 milliliters, K2HPO40.392 grams of glycerine and MgSO4 0.732 in every 1000 milliliters of water
Gram, pH 6.5-7.5.
9. a kind of preparation method of growth-promoting preparation described in claim 1, which is characterized in that include the following steps:(1) it is trained in KB
Streak inoculation Pseudomonas chlororaphis on base is supported, under non-anaerobic environment, -48h for 24 hours is cultivated at 28 DEG C;(2) by Methylotrophic gemma
It is 0.6-0.8 that bacillus, which is cultivated in seed culture medium to strain density OD600 values, bacterium solution is obtained, then in 100 parts by weight culture mediums
The bacterium solution of the Methylotrophic bacillus of middle inoculation 2-5 parts by weight, 37 DEG C of cultures;(3) streak inoculation is glimmering on KB culture mediums
Under non-anaerobic environment, 48h is cultivated at 28 DEG C for light pseudomonad, visible under 254nm ultraviolet lights to have strong yellow green glimmering
Light generates, and cultivates 3-4d;(4) distilled water is added according to the ratio into container, at room temperature sealing a period of time;(4) at room temperature, so
The orange subspecies of addition Pseudomonas chlororaphis, Methylotrophic bacillus and Pseudomonas fluorescens, concussion are equal according to the ratio successively afterwards
It is even, you can.
10. a kind of application of growth-promoting preparation described in claim 1 in preventing ginkgo stem rot.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1772881A (en) * | 2005-10-10 | 2006-05-17 | 中国农业大学 | Fluorescent pseudomonads and its fermenting culture process and application |
| CN101514331A (en) * | 2009-01-05 | 2009-08-26 | 中国农业大学 | Pseudomonas. chlororaphis subsp. Aurantiaca Pa40 and application thereof |
| CN101606541A (en) * | 2009-07-17 | 2009-12-23 | 上海师范大学 | The preparation method of fluorescent pseudomonas microbial pesticide |
| CN103805534A (en) * | 2013-11-28 | 2014-05-21 | 中国农业科学院农业资源与农业区划研究所 | Bacillus methylotrophicus, microorganism bacterium agents and application of bacillus methylotrophicus and microorganism bacterium agents |
| CN105936886A (en) * | 2016-07-15 | 2016-09-14 | 北京市辐射中心 | Bacillus methylothophicus strain for preventing and controlling pear ring rot and soft rot and application of bacillus methylothophicus strain |
-
2018
- 2018-05-12 CN CN201810452044.6A patent/CN108684710B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1772881A (en) * | 2005-10-10 | 2006-05-17 | 中国农业大学 | Fluorescent pseudomonads and its fermenting culture process and application |
| CN101514331A (en) * | 2009-01-05 | 2009-08-26 | 中国农业大学 | Pseudomonas. chlororaphis subsp. Aurantiaca Pa40 and application thereof |
| CN101606541A (en) * | 2009-07-17 | 2009-12-23 | 上海师范大学 | The preparation method of fluorescent pseudomonas microbial pesticide |
| CN103805534A (en) * | 2013-11-28 | 2014-05-21 | 中国农业科学院农业资源与农业区划研究所 | Bacillus methylotrophicus, microorganism bacterium agents and application of bacillus methylotrophicus and microorganism bacterium agents |
| CN105936886A (en) * | 2016-07-15 | 2016-09-14 | 北京市辐射中心 | Bacillus methylothophicus strain for preventing and controlling pear ring rot and soft rot and application of bacillus methylothophicus strain |
Non-Patent Citations (3)
| Title |
|---|
| I. A JDYEA ETAL.: "Evaluation efficiency of Pseudomonas fluorescens and Trichoderma viridi in biological control of Rhizoctonia solani and Macrophomina phaseolina in sesame plant under laboratory and green house conditions", 《JOURNAL OF BIOTECHNOLOGY RESEARCH CENTER》 * |
| RADHAKRISHNANP RAMALINGAM ET AL.: "Isolation and identification of charcoal rot disease causing agent in sesame (Sesamum indicum L.) And their growth inhibition by bacillus methylotrophicus KE2", 《PAKISTAN JOURNAL OF BOTANY》 * |
| 徐志华 等: "《经济树种病害识别与防治》", 31 March 2001, 中国林业出版社 * |
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Application publication date: 20181023 Assignee: Xintian County Tianyuan Chunxiao Ecological Agriculture Co.,Ltd. Assignor: HUNAN University OF SCIENCE AND ENGINEERING Contract record no.: X2023980036440 Denomination of invention: A growth promoting agent and preparation method for preventing ginkgo stem rot disease Granted publication date: 20210625 License type: Common License Record date: 20230612 |