CN108642719A - A kind of apparatus and method of Static Spinning integration small-caliber artificial blood vessel holder - Google Patents
A kind of apparatus and method of Static Spinning integration small-caliber artificial blood vessel holder Download PDFInfo
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- CN108642719A CN108642719A CN201810448418.7A CN201810448418A CN108642719A CN 108642719 A CN108642719 A CN 108642719A CN 201810448418 A CN201810448418 A CN 201810448418A CN 108642719 A CN108642719 A CN 108642719A
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- Prior art keywords
- spinning
- blood vessel
- receiving roll
- fiber
- panels
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Classifications
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/76—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres otherwise than in a plane, e.g. in a tubular way
-
- D—TEXTILES; PAPER
- D10—INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES
- D10B—INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES
- D10B2509/00—Medical; Hygiene
- D10B2509/06—Vascular grafts; stents
Abstract
The invention discloses the devices of Static Spinning integration small-caliber artificial blood vessel holder, including device for spinning, reception device and intravascular stent building mortion；Intravascular stent building mortion includes yarn wind-up device and coiling control device, and yarn wind-up device is used to the fiber that reception device receives being wound into tubular；Coiling control device is moved axially and is rotated in a circumferential direction for control fiber winding device, forms nanofiber pipe, and nanofiber pipe removes receiving roll, obtain integrated small-caliber artificial blood vessel holder by rinsing.The invention also discloses the methods for preparing integrated small-caliber artificial blood vessel holder using above-mentioned apparatus.Apparatus of the present invention are simple in structure, of low cost, and production process is easily operated, efficient；The present invention can control its inner diameter size when preparing small-caliber artificial blood vessel holder by adjusting receiving roll cross-sectional diameter；The small-caliber artificial blood vessel holder being prepared using apparatus of the present invention has the features such as strength is big, and porosity is high, and biocompatibility is good.
Description
Technical field
The invention belongs to electrostatic spinning technique fields, are related to a kind of dress of Static Spinning integration small-caliber artificial blood vessel holder
It sets, the invention further relates to the methods for preparing integrated small-caliber artificial blood vessel holder using above-mentioned apparatus.
Background technology
Human vas includes inner membrance, middle film and outer membrane three-decker.Each layer by different cell compositions, wherein internal layer and
Outer layer and contacting blood should have good biocompatibility and blood compatibility；Middle level need to maintain blood vessel structure, and bear blood
Pipe pressure, therefore should have excellent mechanical property.
Modern society, irregular with diet with the quickening pace of modern life, angiocardiopathy, which has become, threatens the mankind
One big killer of health, is the major reason for causing mankind's high mortality.Vascular reconstruction surgery is to treat having for angiocardiopathy
Efficacious prescriptions method, it is most of all to use self blood because autologous vein has many advantages, such as good biocompatibility, immunity and antigenicity
Pipe is as substitute, but its limited source.Therefore, artificial blood vessel becomes one kind selection well for the treatment of angiocardiopathy.Mesh
Before, heavy caliber artificial blood vessel has been applied to clinic, but for the application of small-caliber artificial blood vessel (internal diameter is less than 6mm) also into
In one step research.The reason is that, when small-bore artificial blood vessel bracket implants, easily forms thrombus and lead to endangium
Hyperplasia.Therefore, ideal artificial blood vessel bracket should have hierarchical structure similar with human vas, and its inner surface copes with blood
Endothelial cell is with stronger adhesion strength to form the generation that fine and close endothelial layer comes formation and the blood coagulation of anti-tampon.
With biotechnology and nanotechnology high speed development, for comparing other preparation methods, Static Spinning Nanowire
There is its peculiar advantage in Wesy in artificial blood vessel bracket, because it can form the tubular structure of required bore and can also imitate
The Nomenclature Composition and Structure of Complexes of extracellular matrix provides good growing environment for cell, and weight is provided for the regeneration and reconstruction of autologous vein
Want premise.
Invention content
It is imitative to prepare the object of the present invention is to provide a kind of device of Static Spinning integration small-caliber artificial blood vessel holder
Raw small-caliber artificial blood vessel holder of good performance.
It is a further object of the present invention to provide the methods for preparing integrated small-caliber artificial blood vessel holder using above-mentioned apparatus.
The technical solution adopted in the present invention is a kind of device of Static Spinning integration small-caliber artificial blood vessel holder, packet
Device for spinning, reception device and intravascular stent building mortion are included, reception device and intravascular stent building mortion are mounted on the base；
Device for spinning includes syringe, and syringe one end is connect by catheter with micro-injection pump, the syringe other end
End is provided with spinning syringe needle, and spinning syringe needle is connect with high voltage direct current generator, and reception device is located at immediately below spinning syringe needle, spinning
Syringe needle is perpendicular to reception device；
Reception device is for receiving the fiber that device for spinning is obtained by Static Spinning；
Intravascular stent building mortion includes yarn wind-up device and coiling control device, and yarn wind-up device will be for that will receive
The fiber that device receives is wound into tubular；Coiling control device is moved axially and is rotated in a circumferential direction for control fiber winding device,
The fiber on yarn wind-up device is set to form intravascular stent structure.
The features of the present invention also characterized in that
Reception device includes two panels receiver board, and receiver board fixation is vertically installed on pedestal, receives plate earthing, spinning syringe needle
Right over the plane that two panels receiver board is formed, the fiber that spinning obtains is overlapped between two panels receiver board.
Two panels receiver board is mutually parallel.
Yarn wind-up device includes receiving roll, receiving roll between two panels receiver board, the material of receiving roll be terylene or
Polytetrafluoroethylene (PTFE).
Coiling control device includes being respectively used to control and receive the control dress that rotates in a circumferential direction that roller is rotated in a circumferential direction and moved axially
Set and move axially control device；
The control device that rotates in a circumferential direction includes variable-frequency motor b, and variable-frequency motor b is mounted on slide plate, variable-frequency motor b and receiving roll
One end connects, and rotates in a circumferential direction for controlling and receiving roller；
Axial movement control device includes the track being set on the base and stroke limit slot, and slide plate is moved along track level
Dynamic, slide plate is connect far from receiving roll one end with motion bar, and motion bar both ends are respectively arranged in stroke limit slot, motion bar and company
Bar one end connects, and the connecting rod other end is provided with spill spin block, and variable-frequency motor a drives spill spin block to rotate in a circumferential direction by mandrel, drives
Connecting rod drives motion bar to be moved in stroke limit slot, to drive slide plate level to move, and then drives receiving roll axial movement.
Another technical solution of the present invention is a kind of preparation method of integration small-caliber artificial blood vessel holder,
A kind of device of used Static Spinning integration small-caliber artificial blood vessel holder, including device for spinning, reception device and blood vessel
Stent forming device, reception device and intravascular stent building mortion are mounted on the base；
Device for spinning includes syringe, and syringe one end is connect by catheter with micro-injection pump, the syringe other end
End is provided with spinning syringe needle, and spinning syringe needle is connect with high voltage direct current generator, and reception device is located at immediately below spinning syringe needle, spinning
Syringe needle is perpendicular to reception device；
Reception device is for receiving the fiber that device for spinning electrospinning obtains；The reception device includes that two panels receives
Plate, receiver board fixation be vertically installed on pedestal, receive plate earthing, spinning syringe needle be located at two panels receiver board formed plane just on
Side, the fiber that spinning obtains are overlapped between two panels receiver board, and two panels receiver board is mutually parallel；
Intravascular stent building mortion includes yarn wind-up device and coiling control device, and yarn wind-up device will be for that will receive
The fiber that device receives is wound into tubular；Coiling control device is moved axially and is rotated in a circumferential direction for control fiber winding device,
The fiber on yarn wind-up device is set to form intravascular stent structure；
Yarn wind-up device includes receiving roll, and receiving roll is between two panels receiver board；
Coiling control device includes being respectively used to control and receive the control dress that rotates in a circumferential direction that roller is rotated in a circumferential direction and moved axially
Set and move axially control device；
The control device that rotates in a circumferential direction includes variable-frequency motor b, and variable-frequency motor b is mounted on slide plate, variable-frequency motor b and receiving roll
One end connects, and rotates in a circumferential direction for controlling and receiving roller；
Axial movement control device includes the track being set on the base and stroke limit slot, and slide plate is moved along track level
Dynamic, slide plate is connect far from receiving roll one end with motion bar, and motion bar both ends are respectively arranged in stroke limit slot, motion bar and company
Bar one end connects, and the connecting rod other end is provided with spill spin block, and variable-frequency motor a drives spill spin block to rotate in a circumferential direction by mandrel, drives
Connecting rod drives motion bar to be moved in stroke limit slot, to drive slide plate level to move, and then drives receiving roll axial movement；
It is specifically implemented according to the following steps：
Step 1, spinning solution is prepared：
At room temperature, the polymer A with biocompatibility is dissolved in solvent A ＇, dissolving stirring is uniform to solution, obtains
The solution A that polymer A mass fractions are 7%~9%；Polymer B with biocompatibility is dissolved in solvent B ＇, dissolving is stirred
Mix it is uniform to solution, obtain polymer B mass fraction be 10%~12% B solution；
Step 2, the nanofiber pipe with real blood vessels structure is prepared：
Step 2.1 prepares blood vessel inner layer tunica fibrosa：
It is 20 DEG C~30 DEG C in temperature, under the environmental condition that relative humidity is 40%~60%, the solution A of preparation is added
In syringe, the distance between setting two panels receiver board；High voltage direct current generator is opened, spinning voltage, spinning distance are adjusted, is adjusted
It saves micro-injection pump and controls solution flow rate；Variable-frequency motor a is opened, rotating speed is adjusted, variable-frequency motor a drives spill spin block to do by mandrel
It rotates in a circumferential direction, and then by connecting rod, motion bar and slide plate, makes receiving roll axially reciprocating；Variable-frequency motor b is opened simultaneously, is adjusted
Rotating speed is saved, driving receiving roll rotates in a circumferential direction；Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect,
It is overlapped between two panels receiver board, along week while the receiving roll between two panels receiver board pulls the movement of fiber axial reciprocating
To rotation, fiber is made to be attached to reception roller surface, obtains blood vessel inner layer tunica fibrosa；
Step 2.2, fiber is made to be attached to step 2.1 blood vessel inner layer fiber as spinning solution using B solution, solution A respectively successively
Film surface, to respectively obtain media tunica fibrosa and blood vessel external tunica fibrosa to get to receiving with real blood vessels structure
Rice fibre pipe；
Step 3, the nanofiber pipe with real blood vessels structure that step 2 obtains is rinsed, removes receiving roll, i.e.,
Obtain integrated small-caliber artificial blood vessel holder.
The features of the present invention also characterized in that
The distance between two panels receiver board be 3cm~5cm, spinning voltage be 10kV~30kV, spinning distance be 100mm~
200mm, solution flow rate are 0.8mL/h~1.5mL/h, a diameter of 2mm~6mm of receiving roll.
Polymer A is fibroin albumen, sulphation fibroin albumen, polyglycolic acid, chitosan, collagen, polyethylene glycol, gathers
One or more mixing in vinyl alcohol；
Polymer B is polycaprolactone, polyurethane, poly- (D-lactic acid-caprolactone), fibroin albumen, polydioxanone
In one or more mixing；
Solvent A ＇ and solvent B ＇ is hexafluoroisopropanol, chloroform, N,N-dimethylformamide, dichloromethane, trifluoro second
One or more mixing in acid, acetone, ethyl alcohol, ethylene glycol.
In step 2, before spinning, in one layer of lubricant of receiving roll surface smear；The rotating speed of variable-frequency motor a be 25rpm~
35rpm；When preparing blood vessel inner layer tunica fibrosa, the rotating speed of variable-frequency motor b is 300rpm~500rpm；Prepare media tunica fibrosa
When with blood vessel external tunica fibrosa, the rotating speed of variable-frequency motor b is 700rpm~900rpm.
The fiber and receiving roll axial direction for forming blood vessel inner layer tunica fibrosa are in less than 5 degree and arranged in parallel；Form media
The fiber of tunica fibrosa is axially vertical and arranged in parallel with receiving roll；The fiber for forming blood vessel external tunica fibrosa axially hangs down with receiving roll
It is straight and arranged in parallel.
The beneficial effects of the invention are as follows：
1. apparatus of the present invention are simple in structure, of low cost, production process is easily operated, efficient；
2. the present invention when preparing small-caliber artificial blood vessel holder, can control it by adjusting receiving roll cross-sectional diameter
Inner diameter size；
3. being had using the small-caliber artificial blood vessel holder that apparatus of the present invention are prepared, strength is big, and porosity is high, biology
The features such as compatibility is good.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram of the device of Static Spinning integration small-caliber artificial blood vessel holder of the present invention；
Fig. 2 is the structural schematic diagram of integrated small-caliber artificial blood vessel holder prepared by the method for the present invention.
In figure, 1. micro-injection pumps, 2. catheters, 3. high voltage direct current generators, 4. syringes, 5. spinning syringe needles, 6. become
Frequency motor a, 7. spill spin blocks, 8. connecting rods, 9. receiving rolls, 10. variable-frequency motor b, 11. stroke limit slots, 12. motion bars, 13. ground connection
Line, 14. receiver boards, 15. slide plates, 16. pedestals, 17. blood vessel inner layer tunica fibrosas, 18. media tunica fibrosas, 19. blood vessel externals
Tunica fibrosa.
Specific implementation mode
The following describes the present invention in detail with reference to the accompanying drawings and specific embodiments.
A kind of device of Static Spinning integration small-caliber artificial blood vessel holder of the present invention, structure is as shown in Figure 1, including spinning
Silk device, reception device and intravascular stent building mortion, reception device and intravascular stent building mortion are mounted on pedestal 16；
Device for spinning includes syringe 4, and 4 one end of syringe is connect by catheter 2 with micro-injection pump 1, and syringe 4 is another
One end end is provided with spinning syringe needle 5, and spinning syringe needle 5 is connect with high voltage direct current generator 3, and reception device is being located at spinning syringe needle 5 just
Lower section, spinning syringe needle 5 is perpendicular to reception device.
Reception device includes the two panels receiver board 14 being mutually parallel being vertically arranged, and receiver board 14 is fixedly mounted on pedestal 16
On, receiver board 14 is connect with ground wire 13, and spinning syringe needle 5 is located at right over the plane of the formation of two panels receiver board 14, and spinning obtains
Fiber be overlapped between two panels receiver board 14.
Intravascular stent building mortion includes yarn wind-up device and coiling control device, and yarn wind-up device will be for that will overlap
Fiber between two panels receiver board 14 is wound into tubular；Coiling control device for control fiber winding device axial movement and
It rotates in a circumferential direction, the fiber on yarn wind-up device is made to form intravascular stent structure.
Yarn wind-up device includes receiving roll 9, and receiving roll 9 is between two panels receiver board 14.The material of receiving roll 9 is to wash
Synthetic fibre or polytetrafluoroethylene (PTFE), a diameter of 2mm~6mm.
Coiling control device includes variable-frequency motor a6 and variable-frequency motor b10, and variable-frequency motor b10 is mounted on slide plate 15, is become
Frequency motor b10 is connect with 9 one end of receiving roll, is rotated in a circumferential direction for controlling and receiving roller 9；Track and stroke limit are provided on pedestal 16
Position slot 11, slide plate 15 are moved horizontally along track, and slide plate 15 is connect far from 9 one end of receiving roll with motion bar 12,12 both ends of motion bar
It is respectively arranged in stroke limit slot 11, motion bar 12 is connect with 8 one end of connecting rod, and 8 other end of connecting rod is provided with spill spin block 7, is become
Frequency motor a6 drives spill spin block 7 to rotate in a circumferential direction by mandrel, and drive link 8 drives motion bar 12 to be moved in stroke limit slot 11
It is dynamic, to drive slide plate 15 to move horizontally, and then receiving roll 9 is driven to move axially.
The method for preparing integrated small-caliber artificial blood vessel holder using above-mentioned apparatus, is specifically implemented according to the following steps：
Step 1, spinning solution is prepared：
At room temperature, the polymer A with biocompatibility is dissolved in solvent A ＇, passes through constant temperature blender with magnetic force, dissolving
Stirring is uniform to solution, obtains the solution A that polymer A mass fractions are 7%~9%；By the polymer B with biocompatibility
It is dissolved in solvent B ＇, by constant temperature blender with magnetic force, dissolving stirring is uniform to solution, and it is 10% to obtain polymer B mass fraction
~12% B solution.
Polymer A is fibroin albumen, sulphation fibroin albumen, polyglycolic acid, chitosan, collagen, polyethylene glycol, gathers
One or more mixing in vinyl alcohol；
Polymer B is polycaprolactone, polyurethane, poly- (D-lactic acid-caprolactone), fibroin albumen, polydioxanone
In one or more mixing；
Solvent A ＇ and solvent B ＇ is hexafluoroisopropanol, chloroform, N,N-dimethylformamide, dichloromethane, trifluoro second
One or more mixing in acid, acetone, ethyl alcohol, ethylene glycol.
Step 2, blood vessel inner layer tunica fibrosa is prepared：
It is 20 DEG C~30 DEG C in temperature, under the environmental condition that relative humidity is 40%~60%, the solution A of preparation is added
In syringe 4, the distance between setting two panels receiver board 14 is 3cm~5cm.One layer of lubrication is smeared before spinning in receiving roll 9
High voltage direct current generator 3 is opened in agent (PEG), and adjusting spinning voltage is 10kV~30kV, adjusts 5 head end of spinning syringe needle and two panels
Vertical range (i.e. spinning distance) between the plane that receiver board 14 is formed is 100mm~200mm, adjusts micro-injection pump 1 and controls
Solution flow rate processed is 0.8mL/h~1.5mL/h.Variable-frequency motor a6 is opened, adjusting rotating speed is 25rpm~35rpm, variable-frequency motor a6
It drives spill spin block 7 to rotate in a circumferential direction by mandrel, and then by connecting rod 8, motion bar 12 and slide plate 15, keeps receiving roll 9 axial past
Multiple movement, while variable-frequency motor b10 is opened, adjusting rotating speed is 300rpm~500rpm, and driving receiving roll 9 rotates in a circumferential direction.Spinning
Liquid sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, is overlapped between two panels receiver board 14, is located at two
The dragging fiber axial reciprocating of receiving roll 9 between piece receiver board 14 is circumferentially rotatable while movement, and fiber is made to be attached to reception
9 surface of roller, it is in the blood vessel inner layer tunica fibrosa 17 less than 5 degree and being mutually parallel that final acquisition fiber is axial with receiving roll 9.
Step 3, media tunica fibrosa is prepared：
It it is 20 DEG C~30 DEG C in temperature, under the environmental condition that relative humidity is 40%~60%, with the internal layer of the 2nd step preparation
Tunica fibrosa is to receive substrate, and the B solution of preparation is added in syringe 4, the distance between setting two panels receiver board 14 for 3cm~
5cm.High voltage direct current generator 3 is opened, adjusting spinning voltage is 10kV~30kV, and spinning distance is 100mm~200mm, is adjusted
It is 0.8mL/h~1.5mL/h that micro-injection pump 1, which controls solution flow rate,.Open variable-frequency motor a6, adjustings rotating speed for 25rpm~
35rpm, variable-frequency motor a6 drive spill spin block 7 to rotate in a circumferential direction by mandrel, and then by connecting rod 8, motion bar 12 and slide plate 15,
Make 9 axially reciprocating of receiving roll, while opening variable-frequency motor b10, adjusting rotating speed is 700rpm~900rpm, drives receiving roll
9 rotate in a circumferential direction.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, is overlapped on two panels receiver board
Between 14, the dragging fiber axial reciprocating of receiving roll 9 between two panels receiver board 14 is circumferentially rotatable while movement, makes fibre
Dimension is attached to 17 surface of blood vessel inner layer tunica fibrosa that step 2 obtains, and final acquisition fiber is axial and flat basically perpendicular to receiving roll
The media tunica fibrosa 18 of row arrangement.
Step 4, blood vessel external tunica fibrosa is prepared：
It it is 20 DEG C~30 DEG C in temperature, under the environmental condition that relative humidity is 40%~60%, with the middle level of the 3rd step preparation
Tunica fibrosa is to receive substrate, and the solution A of preparation is added in syringe 4, and the distance between setting two panels receiver board 14 is 3cm
~5cm.High voltage direct current generator 3 is opened, adjusting spinning voltage is 10kV~30kV, and spinning distance is 100mm~200mm, is adjusted
It is 0.8mL/h~1.5mL/h to save micro-injection pump 1 and control solution flow rate.Open variable-frequency motor a6, adjustings rotating speed for 25rpm~
35rpm, variable-frequency motor a6 drive spill spin block 7 to rotate in a circumferential direction by mandrel, and then by connecting rod 8, motion bar 12 and slide plate 15,
Make 9 axially reciprocating of receiving roll, while opening variable-frequency motor b10, adjusting rotating speed is 700rpm~900rpm, drives receiving roll
9 rotate in a circumferential direction.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, is overlapped on two panels receiver board
Between 14, the dragging fiber axial reciprocating of receiving roll 9 between two panels receiver board 14 is circumferentially rotatable while movement, makes fibre
Dimension is attached to 18 surface of media tunica fibrosa that step 3 obtains, and final acquisition fiber is axial and flat basically perpendicular to receiving roll
The blood vessel external tunica fibrosa 19 of row arrangement is to get to nanofiber pipe.
Step 5, receiving roll is removed：
After the completion of spinning, nanofiber pipe is impregnated 3~5 hours with a concentration of 75% alcohol, then rinsed with PBS buffer solution,
Remaining PEG on rack surface is dissolved, receiving roll 9 is pumped, finally obtains integrated small-caliber artificial blood vessel holder, such as Fig. 2 institutes
Show.
The present invention prepares small-caliber artificial blood vessel holder using homemade electrostatic spinning apparatus, and nanofiber is overlapped on two panels
Between parallel receiver board so that the nanofiber in inner fiber film arranges axially directed, and is arranged between nanofiber
Closely, aperture is smaller between fiber, and porosity is higher, is conducive to the flowing of blood.Nanowire in middle level and outer layer fiber film
Dimension is circumferentially-oriented arrangement, and more regular close, keeps the frictional force between fiber higher, therefore can guarantee intravascular stent strength more
It is high.
The polymer used in the present invention all has good biocompatibility and degradability, the Nanowire being prepared
Dimensional tissue engineering rack has a higher specific surface area and porosity, thus can preferably bionic extracellular matrix structure it is special
Point provides the environment being more suitable for for the growth and breeding of cell.
Embodiment 1
Step 1, spinning solution is prepared
At room temperature, fibroin albumen and the blending of sulphation fibroin albumen are dissolved in hexafluoroisopropanol, pass through temperature constant magnetic stirring
Device, dissolving stirring is transparent to solution, obtains the polymer solution A that mass fraction is 7%；By polycaprolactone be dissolved in chloroform/
N,N-dimethylformamide (7:3), by constant temperature blender with magnetic force, dissolving stirring is transparent to solution, and obtaining mass fraction is
10% polymer B solution.
Step 2, blood vessel inner layer tunica fibrosa is prepared
It is 20 DEG C in temperature, under the environmental condition that relative humidity is 45%, the solution A of preparation is added in syringe, if
It is 3cm to set the distance between two panels receiver board.One layer of lubricant (PEG) is smeared before spinning in receiving roll, opens high direct voltage
Generator, adjusting spinning voltage are 10kV, and it is 100mm to adjust spinning distance, and control solution flow rate is 0.8mL/h.Open frequency conversion
Motor a, adjusting rotating speed are 25rpm, and variable-frequency motor a drives spill spin block to rotate in a circumferential direction by mandrel, and then passes through connecting rod, activity
Bar and slide plate make receiving roll axially reciprocating, while opening variable-frequency motor b, and adjusting rotating speed is 300rpm, driving receiving roll week
To rotation.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, be overlapped on two panels receiver board it
Between, the receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, and fiber is made to adhere to
Roller surface is being received, is finally obtaining fiber with receiving roll axially in the blood vessel inner layer tunica fibrosa for being less than 5 degree and being mutually parallel.
Step 3, media tunica fibrosa is prepared
It is 20 DEG C in temperature, is to receive with inner fiber film prepared by the 2nd step under the environmental condition that relative humidity is 45%
The B solution of preparation is added in syringe substrate, and the distance between setting two panels receiver board is 3cm.Open high direct voltage
Device, adjusting spinning voltage are 10kV, and it is 100mm to adjust spinning distance, and control solution flow rate is 0.8mL/h.Open variable-frequency motor
A, adjusting rotating speed be 25rpm, variable-frequency motor a by mandrel drive spill spin block rotate in a circumferential direction, and then by connecting rod, motion bar and
Slide plate makes receiving roll axially reciprocating, while opening variable-frequency motor b, and adjusting rotating speed is 700rpm, and driving receiving roll is circumferentially revolved
Turn.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, is overlapped between two panels receiver board, position
Receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, and fiber is made to be attached to step
2 obtained blood vessel inner layer fiber film surfaces, the final fiber that obtains is basically perpendicular in the axial and arranged in parallel blood vessel of receiving roll
Layer tunica fibrosa.
Step 4, media tunica fibrosa is prepared
It is 20 DEG C in temperature, is to receive with mean fiber film prepared by the 3rd step under the environmental condition that relative humidity is 45%
The solution A of preparation is added in syringe by substrate, and the distance between setting two panels receiver board is 3cm.Open high direct voltage hair
Raw device, adjusting spinning voltage are 10kV, and it is 100mm to adjust spinning distance, and control solution flow rate is 0.8mL/h.Open variable-frequency electric
Machine a, adjusting rotating speed are 25rpm, and variable-frequency motor a drives spill spin block to rotate in a circumferential direction by mandrel, and then passes through connecting rod, motion bar
And slide plate, make receiving roll axially reciprocating, while opening variable-frequency motor b, adjusting rotating speed is 700rpm, and driving receiving roll is circumferential
Rotation.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, is overlapped between two panels receiver board,
Receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, and fiber is made to be attached to step
Rapid 3 obtained media fiber film surfaces, the final fiber that obtains is basically perpendicular to the axial and arranged in parallel blood vessel of receiving roll
Outer layer fiber film is to get to nanofiber pipe.
Step 5, receiving roll is removed
After the completion of spinning, impregnates nanofiber pipe 3 hours with a concentration of 75% alcohol, then rinsed with PBS buffer solution, dissolve
Remaining PEG, pumps receiving roll on rack surface, finally obtains integrated small-caliber artificial blood vessel holder.
The experimental results showed that there is preferable fiber alignment using the artificial blood vessel bracket that this electrostatic spinning apparatus obtains
And mechanical property.
Embodiment 2
Step 1, spinning solution is prepared
At room temperature, polyglycolic acid is dissolved in hexafluoroisopropanol, by constant temperature blender with magnetic force, dissolving stirring is saturating to solution
It is bright, obtain the polymer solution A that mass fraction is 8%；Polyurethane is dissolved in dichloromethane, it is molten by constant temperature blender with magnetic force
Solution stirring is transparent to solution, obtains the polymer B solution that mass fraction is 11%.
Step 2, blood vessel inner layer tunica fibrosa is prepared
It is 22 DEG C in temperature, under the environmental condition that relative humidity is 55%, the solution A of preparation is added in syringe, if
It is 3.5cm to set the distance between two panels receiver board.One layer of lubricant (PEG) is smeared before spinning in receiving roll, it is high to open direct current
It is 20kV to press generator, adjusting spinning voltage, and it is 150mm to adjust spinning distance, and control solution flow rate is 1.2mL/h.It opens and becomes
Frequency motor a, adjusting rotating speed are 30rpm, and variable-frequency motor a drives spill spin block to rotate in a circumferential direction by mandrel, and then passes through connecting rod, work
Lever and slide plate make receiving roll axially reciprocating, while opening variable-frequency motor b, and adjusting rotating speed is 400rpm, drives receiving roll
It rotates in a circumferential direction.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, is overlapped on two panels receiver board
Between, the receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, keeps fiber attached
It and is receiving roller surface, finally obtain fiber with receiving roll axially in the blood vessel inner layer tunica fibrosa for being less than 5 degree and being mutually parallel.
Step 3, media tunica fibrosa is prepared
It is 22 DEG C in temperature, is to receive with inner fiber film prepared by the 2nd step under the environmental condition that relative humidity is 55%
The B solution of preparation is added in syringe substrate, and the distance between setting two panels receiver board is 3.5cm.Open high direct voltage hair
Raw device, adjusting spinning voltage are 20kV, and it is 150mm to adjust spinning distance, and control solution flow rate is 1.2mL/h.Open variable-frequency electric
Machine a, adjusting rotating speed are 30rpm, and variable-frequency motor a drives spill spin block to rotate in a circumferential direction by mandrel, and then passes through connecting rod, motion bar
And slide plate, make receiving roll axially reciprocating, while opening variable-frequency motor b, adjusting rotating speed is 800rpm, and driving receiving roll is circumferential
Rotation.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, is overlapped between two panels receiver board,
Receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, and fiber is made to be attached to step
Rapid 2 obtained blood vessel inner layer fiber film surfaces, the final fiber that obtains is basically perpendicular to the axial and arranged in parallel blood vessel of receiving roll
Mean fiber film.
Step 4, blood vessel external tunica fibrosa is prepared
It is 22 DEG C in temperature, is to receive with mean fiber film prepared by the 3rd step under the environmental condition that relative humidity is 55%
The solution A of preparation is added in syringe by substrate, and the distance between setting two panels receiver board is 3.5cm.Open high direct voltage
Generator, adjusting spinning voltage are 20kV, and spinning distance is 150mm, and control solution flow rate is 1.2mL/h.Open variable-frequency motor
A, adjusting rotating speed be 30rpm, variable-frequency motor a by mandrel drive spill spin block rotate in a circumferential direction, and then by connecting rod, motion bar and
Slide plate makes receiving roll axially reciprocating, while opening variable-frequency motor b, and adjusting rotating speed is 800rpm, and driving receiving roll is circumferentially revolved
Turn.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, is overlapped between two panels receiver board, position
Receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, and fiber is made to be attached to step
3 obtained media fiber film surfaces, the final fiber that obtains are basically perpendicular to outside the axial and arranged in parallel blood vessel of receiving roll
Layer tunica fibrosa is to get to nanofiber pipe.
Step 5, receiving roll is removed
After the completion of spinning, impregnates nanofiber pipe 4 hours with a concentration of 75% alcohol, then rinsed with PBS buffer solution, dissolve
Remaining PEG, pumps receiving roll on rack surface, finally obtains integrated small-caliber artificial blood vessel holder.
The experimental results showed that there is preferable fiber alignment using the artificial blood vessel bracket that this electrostatic spinning apparatus obtains
And mechanical property.
Embodiment 3
Step 1, spinning solution is prepared
At room temperature, chitosan and collagen blending are dissolved in trifluoroacetic acid, pass through constant temperature blender with magnetic force, dissolving stirring
It is transparent to solution, obtain the polymer solution A that mass fraction is 9%；Poly- (D-lactic acid-caprolactone) is dissolved in acetone, is passed through
Constant temperature blender with magnetic force, dissolving stirring is transparent to solution, obtains the polymer B solution that mass fraction is 12%.
Step 2, blood vessel inner layer tunica fibrosa is prepared
It is 30 DEG C in temperature, under the environmental condition that relative humidity is 60%, the solution A of preparation is added in syringe, if
It is 5cm to set the distance between two panels receiver board.One layer of lubricant (PEG) is smeared before spinning in receiving roll, opens high direct voltage
Generator, adjusting spinning voltage are 30kV, and it is 200mm to adjust spinning distance, and control solution flow rate is 1.5mL/h.Open frequency conversion
Motor a, adjusting rotating speed are 32rpm, and variable-frequency motor a drives spill spin block to rotate in a circumferential direction by mandrel, and then passes through connecting rod, activity
Bar and slide plate make receiving roll axially reciprocating, while opening variable-frequency motor b, and adjusting rotating speed is 500rpm, driving receiving roll week
To rotation.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, be overlapped on two panels receiver board it
Between, the receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, and fiber is made to adhere to
Roller surface is being received, is finally obtaining fiber with receiving roll axially in the blood vessel inner layer tunica fibrosa for being less than 5 degree and being mutually parallel.
Step 3, media tunica fibrosa is prepared
It is 30 DEG C in temperature, is to receive with inner fiber film prepared by the 2nd step under the environmental condition that relative humidity is 60%
The B solution of preparation is added in syringe substrate, and the distance between setting two panels receiver board is 5cm.Open high direct voltage
Device, adjusting spinning voltage are 30kV, and it is 200mm to adjust spinning distance, and control solution flow rate is 1.5mL/h.Open variable-frequency motor
A, adjusting rotating speed be 32rpm, variable-frequency motor a by mandrel drive spill spin block rotate in a circumferential direction, and then by connecting rod, motion bar and
Slide plate makes receiving roll axially reciprocating, while opening variable-frequency motor b, and adjusting rotating speed is 900rpm, and driving receiving roll is circumferentially revolved
Turn.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, is overlapped between two panels receiver board, position
Receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, and fiber is made to be attached to step
2 obtained blood vessel inner layer fiber film surfaces, the final fiber that obtains is basically perpendicular in the axial and arranged in parallel blood vessel of receiving roll
Layer tunica fibrosa.
Step 4, blood vessel external tunica fibrosa is prepared
It is 30 DEG C in temperature, is to receive with mean fiber film prepared by the 3rd step under the environmental condition that relative humidity is 60%
The solution A of preparation is added in syringe by substrate, and the distance between setting two panels receiver board is 5cm.Open high direct voltage hair
Raw device, adjusting spinning voltage are 30kV, and it is 200mm to adjust spinning distance, and control solution flow rate is 1.5mL/h.Open variable-frequency electric
Machine a, adjusting rotating speed are 32rpm, and variable-frequency motor a drives spill spin block to rotate in a circumferential direction by mandrel, and then passes through connecting rod, motion bar
And slide plate, make receiving roll axially reciprocating, while opening variable-frequency motor b, adjusting rotating speed is 900rpm, and driving receiving roll is circumferential
Rotation.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, is overlapped between two panels receiver board,
Receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, and fiber is made to be attached to step
Rapid 3 obtained media fiber film surfaces, the final fiber that obtains is basically perpendicular to the axial and arranged in parallel blood vessel of receiving roll
Outer layer fiber film is to get to nanofiber pipe.
Step 5, receiving roll is removed
After the completion of spinning, impregnates nanofiber pipe 5 hours with a concentration of 75% alcohol, then rinsed with PBS buffer solution, dissolve
Remaining PEG, pumps receiving roll on rack surface, finally obtains integrated small-caliber artificial blood vessel holder.
The experimental results showed that there is preferable fiber alignment using the artificial blood vessel bracket that this electrostatic spinning apparatus obtains
And mechanical property.
Embodiment 4
Step 1, spinning solution is prepared
At room temperature, polyethylene glycol is dissolved in ethyl alcohol, by constant temperature blender with magnetic force, dissolving stirring is transparent to solution, obtains
The polymer solution A that mass fraction is 9%；Polyurethane is blended with fibroin albumen and is dissolved in hexafluoroisopropanol, passes through constant temperature magnetic force
Blender, dissolving stirring is transparent to solution, obtains the polymer B solution that mass fraction is 11%.
Step 2, blood vessel inner layer tunica fibrosa is prepared
It is 25 DEG C in temperature, under the environmental condition that relative humidity is 40%, the solution A of preparation is added in syringe, if
It is 4cm to set the distance between two panels receiver board.One layer of lubricant (PEG) is smeared before spinning in receiving roll, opens high direct voltage
Generator, adjusting spinning voltage are 25kV, and it is 180mm to adjust spinning distance, and control solution flow rate is 1.0mL/h.Open frequency conversion
Motor a, adjusting rotating speed are 28rpm, and variable-frequency motor a drives spill spin block to rotate in a circumferential direction by mandrel, and then passes through connecting rod, activity
Bar and slide plate make receiving roll axially reciprocating, while opening variable-frequency motor b, and adjusting rotating speed is 350rpm, driving receiving roll week
To rotation.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, be overlapped on two panels receiver board it
Between, the receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, and fiber is made to adhere to
Roller surface is being received, is finally obtaining fiber with receiving roll axially in the blood vessel inner layer tunica fibrosa for being less than 5 degree and being mutually parallel.
Step 3, media tunica fibrosa is prepared
It is 25 DEG C in temperature, is to receive with inner fiber film prepared by the 2nd step under the environmental condition that relative humidity is 40%
The B solution of preparation is added in syringe substrate, and the distance between setting two panels receiver board is 4cm.Open high direct voltage
Device, adjusting spinning voltage are 25kV, and it is 180mm to adjust spinning distance, and control solution flow rate is 1.0mL/h.Open variable-frequency motor
A, adjusting rotating speed be 28rpm, variable-frequency motor a by mandrel drive spill spin block rotate in a circumferential direction, and then by connecting rod, motion bar and
Slide plate makes receiving roll axially reciprocating, while opening variable-frequency motor b, and adjusting rotating speed is 750rpm, and driving receiving roll is circumferentially revolved
Turn.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, is overlapped between two panels receiver board, position
Receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, and fiber is made to be attached to step
2 obtained blood vessel inner layer fiber film surfaces, the final fiber that obtains is basically perpendicular in the axial and arranged in parallel blood vessel of receiving roll
Layer tunica fibrosa.
Step 4, blood vessel external tunica fibrosa is prepared
It is 25 DEG C in temperature, is to receive with mean fiber film prepared by the 3rd step under the environmental condition that relative humidity is 40%
The solution A of preparation is added in syringe by substrate, and the distance between setting two panels receiver board is 4cm.Open high direct voltage hair
Raw device, adjusting spinning voltage are 25kV, and it is 180mm to adjust spinning distance, and control solution flow rate is 1.0mL/h.Open variable-frequency electric
Machine a, adjusting rotating speed are 28rpm, and variable-frequency motor a drives spill spin block to rotate in a circumferential direction by mandrel, and then passes through connecting rod, motion bar
And slide plate, make receiving roll axially reciprocating, while opening variable-frequency motor b, adjusting rotating speed is 750rpm, and driving receiving roll is circumferential
Rotation.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, is overlapped between two panels receiver board,
Receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, and fiber is made to be attached to step
Rapid 3 obtained media fiber film surfaces, the final fiber that obtains is basically perpendicular to the axial and arranged in parallel blood vessel of receiving roll
Outer layer fiber film is to get to nanofiber pipe.
Step 5, receiving roll is removed
After the completion of spinning, nanofiber pipe is impregnated 4.5 hours with a concentration of 75% alcohol, then rinsed with PBS buffer solution, it is molten
Remaining PEG on rack surface is solved, receiving roll is pumped, finally obtains integrated small-caliber artificial blood vessel holder.
The experimental results showed that there is preferable fiber alignment using the artificial blood vessel bracket that this electrostatic spinning apparatus obtains
And mechanical property.
Embodiment 5
Step 1, spinning solution is prepared
At room temperature, polyvinyl alcohol is dissolved in ethylene glycol, by constant temperature blender with magnetic force, dissolving stirring is transparent to solution, obtains
The polymer solution A for being 8% to mass fraction；Polydioxanone is dissolved in hexafluoroisopropanol, passes through temperature constant magnetic stirring
Device, dissolving stirring is transparent to solution, obtains the polymer B solution that mass fraction is 12%.
Step 2, blood vessel inner layer tunica fibrosa is prepared
It is 28 DEG C in temperature, under the environmental condition that relative humidity is 50%, the solution A of preparation is added in syringe, if
It is 4.5cm to set the distance between two panels receiver board.One layer of lubricant (PEG) is smeared before spinning in receiving roll, it is high to open direct current
It is 22kV to press generator, adjusting spinning voltage, and it is 160mm to adjust spinning distance, and control solution flow rate is 1.3mL/h.It opens and becomes
Frequency motor a, adjusting rotating speed are 35rpm, and variable-frequency motor a drives spill spin block to rotate in a circumferential direction by mandrel, and then passes through connecting rod, work
Lever and slide plate make receiving roll axially reciprocating, while opening variable-frequency motor b, and adjusting rotating speed is 450rpm, drives receiving roll
It rotates in a circumferential direction.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, is overlapped on two panels receiver board
Between, the receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, keeps fiber attached
It and is receiving roller surface, finally obtain fiber with receiving roll axially in the blood vessel inner layer tunica fibrosa for being less than 5 degree and being mutually parallel.
Step 3, media tunica fibrosa is prepared
It is 28 DEG C in temperature, is to receive with inner fiber film prepared by the 2nd step under the environmental condition that relative humidity is 50%
The B solution of preparation is added in syringe substrate, and the distance between setting two panels receiver board is 4.5cm.Open high direct voltage hair
Raw device, adjusting spinning voltage are 22kV, and it is 160mm to adjust spinning distance, and control solution flow rate is 1.3mL/h.Open variable-frequency electric
Machine a, adjusting rotating speed are 35rpm, and variable-frequency motor a drives spill spin block to rotate in a circumferential direction by mandrel, and then passes through connecting rod, motion bar
And slide plate, make receiving roll axially reciprocating, while opening variable-frequency motor b, adjusting rotating speed is 850rpm, and driving receiving roll is circumferential
Rotation.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, is overlapped between two panels receiver board,
Receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, and fiber is made to be attached to step
Rapid 2 obtained blood vessel inner layer fiber film surfaces, the final fiber that obtains is basically perpendicular to the axial and arranged in parallel blood vessel of receiving roll
Mean fiber film.
Step 4, blood vessel external tunica fibrosa is prepared
It is 28 DEG C in temperature, is to receive with mean fiber film prepared by the 3rd step under the environmental condition that relative humidity is 50%
The solution A of preparation is added in syringe by substrate, and the distance between setting two panels receiver board is 4.5cm.Open high direct voltage
Generator, adjusting spinning voltage are 22kV, and it is 160mm to adjust spinning distance, and control solution flow rate is 1.3mL/h.Open frequency conversion
Motor a, adjusting rotating speed are 35rpm, and variable-frequency motor a drives spill spin block to rotate in a circumferential direction by mandrel, and then passes through connecting rod, activity
Bar and slide plate make receiving roll axially reciprocating, while opening variable-frequency motor b, and adjusting rotating speed is 850rpm, driving receiving roll week
To rotation.Spinning solution sprays the jet stream to be formed and is drafted into nanofiber under electric field force effect, be overlapped on two panels receiver board it
Between, the receiving roll dragging fiber axial reciprocating between two panels receiver board is circumferentially rotatable while movement, and fiber is made to adhere to
In the media fiber film surface that step 3 obtains, final acquisition fiber is axial and arranged in parallel basically perpendicular to receiving roll
Blood vessel external tunica fibrosa is to get to nanofiber pipe.
Step 5, receiving roll is removed
After the completion of spinning, nanofiber pipe is impregnated 3.5 hours with a concentration of 75% alcohol, then rinsed with PBS buffer solution, it is molten
Remaining PEG on rack surface is solved, receiving roll is pumped, finally obtains integrated small-caliber artificial blood vessel holder.
The experimental results showed that there is preferable fiber alignment using the artificial blood vessel bracket that this electrostatic spinning apparatus obtains
And mechanical property.
Claims (10)
1. a kind of device of Static Spinning integration small-caliber artificial blood vessel holder, which is characterized in that including device for spinning, receive dress
It sets and is mounted on pedestal (16) with intravascular stent building mortion, reception device and intravascular stent building mortion；
Device for spinning includes syringe (4), and syringe (4) one end is connect by catheter (2) with micro-injection pump (1), injection
Device (4) other end end is provided with spinning syringe needle (5), and spinning syringe needle (5) is connect with high voltage direct current generator (3), reception device position
Immediately below spinning syringe needle (5), spinning syringe needle (5) is perpendicular to reception device；
Reception device is for receiving the fiber that device for spinning is obtained by Static Spinning；
Intravascular stent building mortion includes yarn wind-up device and coiling control device, and yarn wind-up device is used for reception device
The fiber of reception is wound into tubular；Coiling control device is moved axially and is rotated in a circumferential direction for control fiber winding device, makes volume
The fiber being wound on yarn wind-up device forms intravascular stent structure.
2. a kind of device of Static Spinning integration small-caliber artificial blood vessel holder according to claim 1, which is characterized in that
The reception device includes two panels receiver board (14), and receiver board (14) fixation is vertically installed on pedestal (16), receiver board (14)
Ground connection, spinning syringe needle (5) are located at right over the plane of two panels receiver board (14) formation, and the fiber that spinning obtains is overlapped on two panels and connects
Between receipts plate (14).
3. a kind of device of Static Spinning integration small-caliber artificial blood vessel holder according to claim 2, which is characterized in that
The two panels receiver board (14) is mutually parallel.
4. a kind of device of Static Spinning integration small-caliber artificial blood vessel holder according to claim 2, which is characterized in that
The yarn wind-up device includes receiving roll (9), and receiving roll (9) is located between two panels receiver board (14), the material of receiving roll (9)
For terylene or polytetrafluoroethylene (PTFE).
5. a kind of device of Static Spinning integration small-caliber artificial blood vessel holder according to claim 4, which is characterized in that
The coiling control device includes being respectively used to control and receive the control device that rotates in a circumferential direction that roller (9) is rotated in a circumferential direction and moved axially
With axial movement control device；
The control device that rotates in a circumferential direction includes (10) variable-frequency motor b, and variable-frequency motor b (10) is mounted on slide plate (15), frequency conversion
Motor b (10) is connect with receiving roll (9) one end, is rotated in a circumferential direction for controlling and receiving roller (9)；
The axial movement control device includes the track and stroke limit slot (11) being arranged on pedestal (16), slide plate (15) edge
Track moves horizontally, and slide plate (15) is connect far from receiving roll (9) one end with motion bar (12), and motion bar (12) both ends are installed respectively
In stroke limit slot (11), motion bar (12) is connect with connecting rod (8) one end, and connecting rod (8) other end is provided with spill spin block (7),
Variable-frequency motor a (6) drives spill spin block (7) to rotate in a circumferential direction by mandrel, and drive link (8) drives motion bar (12) to be expert at degree
It is moved in position slot (11), to drive slide plate (15) to move horizontally, and then receiving roll (9) is driven to move axially.
6. a kind of preparation method of integration small-caliber artificial blood vessel holder, which is characterized in that used a kind of Static Spinning one
The device of body small-caliber artificial blood vessel holder, including device for spinning, reception device and intravascular stent building mortion, reception device
It is mounted on pedestal (16) with intravascular stent building mortion；
Device for spinning includes syringe (4), and syringe (4) one end is connect by catheter (2) with micro-injection pump (1), injection
Device (4) other end is provided with spinning syringe needle (5), and spinning syringe needle (5) is connect with high voltage direct current generator (3), and reception device is located at
Immediately below spinning syringe needle (5), spinning syringe needle (5) is perpendicular to reception device；
Reception device is for receiving the fiber that device for spinning is obtained by Static Spinning；The reception device includes two panels receiver board
(14), receiver board (14) fixation is vertically installed on pedestal (16), and receiver board (14) ground connection, spinning syringe needle (5) connects positioned at two panels
It receives right over the plane that plate (14) is formed, the fiber that spinning obtains is overlapped between two panels receiver board (14), two panels receiver board
(14) it is mutually parallel；
Intravascular stent building mortion includes yarn wind-up device and coiling control device, and yarn wind-up device is used for reception device
The fiber of reception is wound into tubular；Coiling control device is moved axially and is rotated in a circumferential direction for control fiber winding device, makes volume
The fiber being wound on yarn wind-up device forms intravascular stent structure；
The yarn wind-up device includes receiving roll (9), and receiving roll (9) is located between two panels receiver board (14)；
The coiling control device includes being respectively used to control and receive the control that rotates in a circumferential direction that roller (9) is rotated in a circumferential direction and moved axially
Device and axial movement control device；
The control device that rotates in a circumferential direction includes (10) variable-frequency motor b, and variable-frequency motor b (10) is mounted on slide plate (15), frequency conversion
Motor b (10) is connect with receiving roll (9) one end, is rotated in a circumferential direction for controlling and receiving roller (9)；
The axial movement control device includes the track and stroke limit slot (11) being arranged on pedestal (16), slide plate (15) edge
Track moves horizontally, and slide plate (15) is connect far from receiving roll (9) one end with motion bar (12), and motion bar (12) both ends are installed respectively
In stroke limit slot (11), motion bar (12) is connect with connecting rod (8) one end, and connecting rod (8) other end is provided with spill spin block (7),
Variable-frequency motor a (6) drives spill spin block (7) to rotate in a circumferential direction by mandrel, and drive link (8) drives motion bar (12) to be expert at degree
It is moved in position slot (11), to drive slide plate (15) to move horizontally, and then receiving roll (9) is driven to move axially；
It is specifically implemented according to the following steps：
Step 1, spinning solution is prepared：
At room temperature, the polymer A with biocompatibility is dissolved in solvent A ＇, dissolving stirring is uniform to solution, is polymerize
The solution A that object A mass fractions are 7%~9%；Polymer B with biocompatibility is dissolved in solvent B ＇, dissolving stirring is extremely
Solution is uniform, obtains the B solution that polymer B mass fraction is 10%~12%；
Step 2, the nanofiber pipe with real blood vessels structure is prepared：
Step 2.1 prepares blood vessel inner layer tunica fibrosa：
It is 20 DEG C~30 DEG C in temperature, under the environmental condition that relative humidity is 40%~60%, injection is added in the solution A of preparation
In device (4), the distance between setting two panels receiver board (14)；High voltage direct current generator (3) is opened, spinning voltage, spinning are adjusted
Distance adjusts micro-injection pump (1) and controls solution flow rate；Variable-frequency motor a (6) is opened, rotating speed is adjusted, variable-frequency motor a (6) passes through
Mandrel drives spill spin block (7) to rotate in a circumferential direction, and then by connecting rod (8), motion bar (12) and slide plate (15), makes receiving roll (9)
Axially reciprocating；Variable-frequency motor b (10) is opened simultaneously, adjusts rotating speed, and driving receiving roll (9) rotates in a circumferential direction；Spinning solution sprays
The jet stream of formation is drafted into nanofiber under electric field force effect, is overlapped between two panels receiver board (14), is connect positioned at two panels
Receiving roll (9) dragging fiber axial reciprocating between receipts plate (14) is circumferentially rotatable while movement, and fiber is made to be attached to reception
Roller (9) surface obtains blood vessel inner layer tunica fibrosa；
Step 2.2, fiber is made to be attached to step 2.1 blood vessel inner layer tunica fibrosa table as spinning solution using B solution, solution A respectively successively
Face, to respectively obtain media tunica fibrosa and blood vessel external tunica fibrosa to get to the Nanowire with real blood vessels structure
Dimension pipe；
Step 3, the nanofiber pipe with real blood vessels structure that step 2 obtains is rinsed, removal receiving roll (9), i.e.,
Obtain integrated small-caliber artificial blood vessel holder.
7. a kind of preparation method of integrated small-caliber artificial blood vessel holder according to claim 6, which is characterized in that institute
State the distance between two panels receiver board (14) be 3cm~5cm, spinning voltage be 10kV~30kV, spinning distance be 100mm~
200mm, solution flow rate are 0.8mL/h~1.5mL/h, a diameter of 2mm~6mm of receiving roll (9).
8. a kind of preparation method of integrated small-caliber artificial blood vessel holder according to claim 6, which is characterized in that institute
It is fibroin albumen, sulphation fibroin albumen, polyglycolic acid, chitosan, collagen, polyethylene glycol, polyvinyl alcohol to state polymer A
In one or more mixing；
Polymer B is in polycaprolactone, polyurethane, poly- (D-lactic acid-caprolactone), fibroin albumen, polydioxanone
One or more mixing；
Solvent A ＇ and solvent B ＇ is hexafluoroisopropanol, chloroform, N,N-dimethylformamide, dichloromethane, trifluoroacetic acid, third
One or more mixing in ketone, ethyl alcohol, ethylene glycol.
9. a kind of preparation method of integrated small-caliber artificial blood vessel holder according to claim 6, which is characterized in that institute
It states in step 2, before spinning, in one layer of lubricant of receiving roll (9) surface smear；The rotating speed of variable-frequency motor a (6) be 25rpm~
35rpm；When preparing blood vessel inner layer tunica fibrosa, the rotating speed of variable-frequency motor b (10) is 300rpm~500rpm；It is fine to prepare media
When tieing up film and blood vessel external tunica fibrosa, the rotating speed of variable-frequency motor b (10) is 700rpm~900rpm.
10. a kind of preparation method of integrated small-caliber artificial blood vessel holder according to claim 6, which is characterized in that
The fiber of the composition blood vessel inner layer tunica fibrosa is with receiving roll (9) axially in less than 5 degree and arranged in parallel；It is fine to form media
Fiber and the receiving roll (9) for tieing up film are axially vertical and arranged in parallel；Form the fiber and receiving roll (9) axis of blood vessel external tunica fibrosa
To vertical and arranged in parallel.
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| CN109700562A (en) * | 2018-12-13 | 2019-05-03 | 贵州大学 | Bionical native blood vessels structure and the multilayer double orientation bracket of function and preparation method thereof |
| CN109722792A (en) * | 2018-12-17 | 2019-05-07 | 河北久丰节能科技有限公司 | A kind of cellucotton pipe automated production equipment |
| CN110481019A (en) * | 2019-08-20 | 2019-11-22 | 广州帷幄生物科技有限公司 | A kind of artificial blood vessel printing device |
| CN111793899A (en) * | 2020-04-30 | 2020-10-20 | 杭州医学院 | Bionic nanofiber material and preparation method and application thereof |
| US20230082048A1 (en) * | 2021-09-15 | 2023-03-16 | Shaanxi Environmental Protection Research Institute | Electrospun nanofiber membrane and method for preparing electrospun nanofiber membrane |
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Effective date of registration: 20220701 Address after: 300000 zone a, No. 10, Hongtong Road, Zhongbei Industrial Park (South Park), Zhongbei Town, Xiqing District, Tianjin Patentee after: Yunfan (Tianjin) Instrument Co.,Ltd. Address before: 710048 No. 19 Jinhua South Road, Shaanxi, Xi'an Patentee before: XI'AN POLYTECHNIC University |