CN105755554A - Small-diameter artificial vascular graft preparation method based on electrostatic spinning - Google Patents
Small-diameter artificial vascular graft preparation method based on electrostatic spinning Download PDFInfo
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- CN105755554A CN105755554A CN201610200049.0A CN201610200049A CN105755554A CN 105755554 A CN105755554 A CN 105755554A CN 201610200049 A CN201610200049 A CN 201610200049A CN 105755554 A CN105755554 A CN 105755554A
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/04—Hollow or tubular parts of organs, e.g. bladders, tracheae, bronchi or bile ducts
- A61F2/06—Blood vessels
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D1/00—Treatment of filament-forming or like material
- D01D1/02—Preparation of spinning solutions
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0069—Electro-spinning characterised by the electro-spinning apparatus characterised by the spinning section, e.g. capillary tube, protrusion or pin
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid
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- Textile Engineering (AREA)
- Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Pulmonology (AREA)
- Cardiology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Prostheses (AREA)
Abstract
The invention provides a small-diameter artificial vascular graft preparation method based on electrostatic spinning. The method includes: preparing a polymer spinning solution; filling a spinning solution injection device with the prepared spinning solution and regulating a spinning solution flow speed; selecting a corresponding receiving bar according to preparation requirements and mounting the receiving bar on a rotary receiving device; adjusting a receiving distance; enabling the receiving bar to ground, turning on a high-voltage static generator to regulate static spinning voltage, and providing a high field between a nozzle and the receiving bar through a high-voltage power source; powering off after preparation is completed, demounting the receiving bar and taking down an artificial vascular graft prepared by spinning. The small-diameter artificial vascular graft preparation method based on electrostatic spinning is simple, and the inner wall of the artificial vascular graft prepared based on electrostatic spinning is provided with a spiral groove, so that flow wall shear force in the artificial vascular graft can be enhanced, harmful substances deposited in blood on the artificial vascular graft wall are reduced, and long-term graft patency of the artificial vascular graft, particularly the small-diameter artificial vascular graft, is improved.
Description
Technical field
The present invention relates to the preparation field of artificial blood vessel, particularly relate to a kind of small-caliber artificial blood vessel preparation method based on electrostatic spinning.
Background technology
The sickness rate of the disease such as coronary heart disease, atherosclerosis increases day by day in recent years, and artificial vascular graft is as the Main Means attention for the treatment of angiopathy.Electrostatic spinning side's technology is widely used in preparing tissue engineering bracket after within 1934, being invented by American Formhals (Formhals) always, the artificial blood vessel prepared by electrospun fibers, having porosity height, specific surface area is big, fiber fine degree and the advantage such as homogeneity is high, draw ratio is big.These good characteristics that cannot obtain by traditional method, impart electrospun fibers and are widely applied prospect.Additionally, the advantage such as electrostatic spinning technique also has quick, efficient, and equipment is simple, easily operated, can be used for that preparation is complicated, exempt to sew up support, be control high-molecular biologic goods pattern, porosity and regulate the effective way of component.
Even to this day, PETE artificial blood vessel and Dacron artificial blood vessel are at heavy caliber (> 6mm) reconstructing blood vessel acquirement immense success, within 5 years, patency rate is up to 91-95%.But, small-bore (≤6mm) artificial blood vessel that application PTFE and Dacron makes but encounters a lot of problem, as very low in long-term patency rate, thrombosis, thromboembolism and serious neointimal hyperplasia, within 5 years, patency rate is low to moderate 43-45%, and these crucial application problems of small-caliber artificial blood vessel are urgently to be resolved hurrily.
Disclosed in patent CN1817328A, a kind of inside pipe wall at blood vessel designs a spiral fluted artificial blood vessel, the geometry of wall inner surface changes the Flow Field Distribution in small-caliber artificial blood vessel, vessel velocity is made to redistribute, improve the flowing boundary shear stress in artificial blood vessel as much as possible, reach to improve the purpose of the long-term patency rate of artificial blood vessel's particularly small-caliber artificial blood vessel, the proposition of this scheme of the invention is to utilize computer hydrodynamics software to simulate the conclusion drawn, but does not clearly provide a kind of means how to realize or scheme.
To sum up, band spiral fluted small-caliber artificial blood vessel to be realized, at least need to solve problems with:
First, which kind of adopts receive rod to receive spinning so that weaving obtains artificial blood vessel and has helicla flute wall construction；
Second, how new receiving arranges syringe needle and the position relationship received between rod under rod；
3rd, how to make the patency rate of small-caliber vascular prepared more excellent.
Summary of the invention
In view of the foregoing, it is an object to provide a kind of small-caliber artificial blood vessel preparation method based on electrostatic spinning, for solving the problem that prior art cannot prepare the small-caliber artificial blood vessel with spiral cell wall.
For achieving the above object and other relevant purposes, the present invention provides techniques below scheme:
A kind of small-caliber artificial blood vessel preparation method based on electrostatic spinning, described method is applied in following device: high-voltage power apparatus, includes the high voltage power supply of positive and negative polarity for exporting at least one road；Spinning solution injection device, including for holding the injection device of spinning solution and exporting the injected current amount control device of spinning solution flow velocity size for regulation and control injection device, for accurately being exported according to the regulation and control of injected current amount control device by the spinning solution in injection device, the syringe needle on injection device is also connected electrically in the positive polarity of the high voltage power supply that high-voltage power apparatus exports；Receive device, including for receiving receiving rod and supplying to control to receive the rotary power control device that rod rotates of electrostatic spinning, receive rod and control device realizes forward or reverse motion by being connected to rotary power, and it is connected electrically in the negative polarity of the high voltage power supply that high-voltage power apparatus exports, wherein, receiving rod is conductor and its cylindrically shaped barred body, and is provided with screw thread on cylindrical bar body side surface；Injection slide unit controls device, it is arranged on reception device side, for regulating syringe needle on injection device and receiving interrod spacing and angle of inclination, to set up between rod for spinning solution by forming the high-voltage electrostatic field of nanometer filamentary fibers at syringe needle and receiving, and it is used for controlling syringe needle and controls device at injection slide unit and move reciprocatingly；The step that realizes of described method includes:
Weighing Pfansteihl and epsilon-caprolactone copolymer P (LLA-CL), be dissolved in hexafluoroisopropanol, stirring vibration, to dissolving completely, obtains the electrostatic spinning solution that concentration is 6-12%；
This electrostatic spinning solution is put in spinning solution injection device, by injected current amount control device therein, the fltting speed of fuse solution is set at 0.8-2.0ml/h, regulate the receiving range most 10-20cm received between rod and syringe needle, select suitable syringe needle, and voltage high voltage power supply set is set to 8-15kv；
Start injection slide unit control device medium power control system and the rotary power received in device controls device, allowing reception rod receive described nanofiber, shape spinning carrying out artificial blood vessel, spinning carries out power-off after 4-8h, unload reception rod, from receiving, rod takes off the artificial blood vessel with inner spiral groove.
0.4gL-lactic acid and epsilon-caprolactone copolymer P (LLA-CL) is weighed with electronic balance, mol ratio is 50:50, it is dissolved in the hexafluoroisopropanol (linear formula: (CF3) 2CHOH) of 5ml, by magnetic stirrer vibration to dissolving completely, generally the rotating speed of magnetic stirring apparatus is set to 100-600rpm, obtains the electrostatic spinning solution that concentration is 6-10% (g/ml)；
This electrostatic spinning solution is put in spinning solution injection device, by injected current amount control device therein, the fltting speed of fuse solution is set at about 1.0ml/h, regulate receiving range most about the 15cm received between rod and syringe needle, No. 8 syringe needles selected by syringe needle, and voltage high voltage power supply set is set to 12kv, obtain P (LLA-CL) nanofiber that average diameter is about 120nm；
Start injection slide unit control device medium power control system and the rotary power received in device controls device, reception rod is allowed to receive described P (LLA-CL) nanofiber, shape spinning carrying out artificial blood vessel, spinning carries out power-off after 4-8h, unload reception rod, from receiving, rod takes off the artificial blood vessel with inner spiral groove.
Preferably, injection slide unit control device at least includes: swing arm；Porous connecting row support, is movably connected on one end of swing arm, including multiple through holes side by side, for the syringe needle of different model is individually fixed in the plurality of through hole side by side；Slide unit, including two spaced limit switches with at the reciprocating slide in this interval, slide is fixedly connected on the other end of swing arm；Power control system, is connected to described slide and limit switch, is used for the slide receiving the transmission of any of which limit switch near signal to drive slide to move to another limit switch direction.
Preferably, injection device is syringe, and injected current amount control device is syringe pump.
Preferably, rotary power controls device and is at least made up of motor and fixing seat, and interval is just arranged by fixing seat and motor, and the rotating shaft of motor and the one end receiving rod are achieved a fixed connection by chuck, and the other end receiving rod is movably connected on fixing seat.
Preferably, described screw thread is projection spiral bar on cylindrical bar body side surface, the helix of this spiral bar stretches in the axial direction on described side, spiral bar is at the outer convex arch being shaped as 120 °-180 ° received on rod section along vertical axis, it is 1-6mm that spiral bar place receives the outer diameter D of rod, spiral bar height h on side is 0.2-0.6mm, and the helix pitch m of spiral bar is 2-6mm.
Preferably, described screw thread is recessed helicla flute on cylindrical bar body side surface, spiral fluted helix stretches in the axial direction on described side, helicla flute is in the indent circular arc being shaped as 120 °-180 ° received on rod section along vertical axis, it is 1-6mm that helicla flute place receives the outer diameter D of rod, spiral fluted degree of depth h is 0.2-0.6mm, and spiral fluted helix pitch m is 2-6mm.
Preferably, described screw thread is by recessed helicla flute on cylindrical bar body side surface and twines the real silk being located on helicla flute and constitutes, spiral fluted helix stretches in the axial direction on described side, it is 1-6mm that helicla flute place receives the outer diameter D of rod, spiral fluted degree of depth h is 0.2-0.6mm, and spiral fluted helix pitch m is 2-6mm.
Preferably, helicla flute is in the indent circular arc being shaped as 120 °-180 ° received on rod section along vertical axis, and real silk twines and is located in helicla flute and forms bottom for having the helicla flute of real silk.
Preferably, real silk is wound around along helicla flute and is covered on helicla flute, is formed over the arc surface protruding cylindrical bar body side surface at helicla flute.
Preferably, the cambered surface angle of arc surface is 120 °-180 °.
As it has been described above, the method have the advantages that
The small-caliber artificial blood vessel with threaded interior structure can be prepared, for first there being theory to provide technical support so that it is can commercialization by said apparatus provided by the invention；
In addition, artificial blood vessel's inwall that the present invention prepares based on electrostatic spinning technique is with helicla flute, the geometry of its wall inner surface changes the Flow Field Distribution in artificial blood vessel, blood flow rate is made to redistribute, increase near wall blood flow rate, cause washing away of artificial blood vessel's wall, under the premise that physiology blood flow is certain, improve the flowing boundary shear stress in artificial blood vessel, in reduction blood, harmful substance is in the deposition of tube wall, reach the purpose of the long-term patency rate improving artificial blood vessel's particularly small-caliber artificial blood vessel, thus for solve small-caliber artificial blood vessel acute embolic lost efficacy this problem being outstanding for a long time provide effective measures.
Accompanying drawing explanation
In order to be illustrated more clearly that the scheme in the embodiment of the present invention, below the accompanying drawing used required described in specific embodiment is briefly described, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those skilled in the art, under the premise not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the principle schematic of a kind of small-caliber artificial blood vessel preparation facilities based on electrostatic spinning.
Fig. 2-1 is a kind of embodiment principle schematic receiving rod.
Fig. 2-2 receives rod complete section schematic diagram on vertical axis direction in Fig. 2-1.
Fig. 3-1 is the another embodiment principle schematic receiving rod.
Fig. 3-2 receives rod complete section schematic diagram on vertical axis direction in Fig. 3-1.
Fig. 4-1 is another the embodiment principle schematic receiving rod.
Fig. 4-2 receives rod complete section schematic diagram on vertical axis direction in Fig. 4-1.
Fig. 5 is that a kind of device utilized in embodiment 1 is to realize the flow chart of method prepared by artificial blood vessel.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based on embodiments of the invention, all other embodiments that those of ordinary skill in the art obtain under the premise not making creative work, broadly fall into the scope of protection of the invention.
Embodiment 1
Refer to 1, for the principle schematic of a kind of small-caliber artificial blood vessel preparation facilities based on electrostatic spinning provided by the invention, this device will be described in detail below.
As it can be seen, this device includes high-voltage power apparatus 1, spinning solution injection device 2, receives device 4 and injection slide unit control device 3, wherein, high-voltage power apparatus 1 includes the high voltage power supply of positive and negative polarity for exporting at least one road；Spinning solution injection device 2 includes for holding the injection device 22 of spinning solution and exporting the injected current amount control device 23 of spinning solution flow velocity size for regulation and control injection device 22, for accurately being exported according to the regulation and control of injected current amount control device 23 by the spinning solution in injection device 22, the syringe needle 21 on injection device 22 is also connected electrically in the positive polarity of the high voltage power supply that high-voltage power apparatus 1 exports；Receive device 4 and include for receiving receiving excellent 41 and controlling device for the rotary power controlling to receive rod 41 rotation of electrostatic spinning, receive rod 41 and control device realizes forward or reverse motion by being connected to rotary power, and it is connected electrically in the negative polarity of the high voltage power supply that high-voltage power apparatus 1 exports, wherein, receiving rod 41 is conductor and its cylindrically shaped barred body 411, and is provided with screw thread on cylindrical barred body 411 side；Injection slide unit controls device 3 and is arranged on reception device 4 side, for regulating syringe needle on injection device 22 21 and receiving excellent 41 spacing and angle of inclination, to set up, between syringe needle 21 and reception rod 41, the high-voltage electrostatic field passing through formation nanometer filamentary fibers for spinning solution, and be used for controlling syringe needle 21 and control device 3 at injection slide unit and move reciprocatingly, the preparation of small-caliber artificial blood vessel with spiral inwall can be realized by said apparatus.
In being embodied as, injection device 22 can adopt syringe, is generally the syringe with syringe needle 21, and injected current amount control device 23 can adopt syringe pump.Connect the syringe to syringe pump can well control the fltting speed of syringe, then control spinning solution output speed in syringe.
In being embodied as, rotary power is controlled device and can be realized by a motor 42a and fixing seat 42b, wherein, interval is just arranged by fixing seat 42b and motor 42a, the rotating shaft of motor 42a and the one end receiving rod 41 are achieved a fixed connection by chuck, the other end receiving rod 41 is movably connected on fixing seat 42b, and receiving rod 41 can also be movably connected on fixing seat 42b by chuck, but now chuck one end should with fixing seat 42b for being flexibly connected.It has been attached several benefit: first by chuck; in view of the length and bore that receive rod 41 itself are less; need when mounting and dismounting to consider the dismounting to artificial blood vessel and protection; once disassembling is become complexity can easily touch artificial blood vessel; the present invention adopts the mode of chuck to have only to loosen chuck can realize dismounting; as long as contrary locking chuck can realize installing, very convenient and swift, also it is not easy to hurt artificial blood vessel.
In being embodied as, injection slide unit controls device 3 and at least includes: swing arm 32, porous connecting row support 31, slide unit 33 and power control system 34, wherein, porous connecting row support 31 is movably connected on one end of swing arm 32, including multiple through holes side by side, for the syringe needle 21 of different model is individually fixed in the plurality of through hole side by side；Slide unit 33 includes two spaced limit switches 331 and for the reciprocating slide 332 at this interval, and slide 332 is fixedly connected on the other end of swing arm 32；Power control system 34 is connected to described slide 332 and limit switch 331, is used for the slide 332 receiving any of which limit switch 331 transmission near signal to drive slide 332 to move to another limit switch 331 direction.Syringe needle 21 can be regulated by injecting slide unit control device 3 and receive the spacing receiving between rod 41 on device 4, by the flexible connection of porous connecting row support 31 and swing arm 32, porous connecting row support 31 can also be rotated, in order to regulate syringe needle 21 and the angle of inclination received between rod 41.It will be appreciated that, receiving rod 41 with syringe needle 21 can not be keep in vertical direction being vertically arranged, need to keep the inclination of certain angle, this is because under being vertically arranged situation, syringe needle 21 is likely to occur dropping liquid situation, drop drops to will damage, on reception rod 41, the blood vessel spinned, and this is necessarily for extremely important the preparation of artificial blood vessel.
In addition, porous connecting row support 31 has multiple fixing hole for fixed needle 21, can be used to the syringe needle 21 by multiple different output gauge be fixed on porous connecting row support 31, and can be undertaken being connected in the positive polarity of high voltage power supply by all syringe needles 21.Furthermore, it is also possible to by regulating syringe needle 21 by swing arm 32 and porous connecting row support 31 and receiving the spacing of rod 41, in prepared by reality, there is good convenience.
More specifically, can in conjunction with Fig. 1, slide 332 in slide unit 33 can by being socketed on guide rod 333 and being arranged between two limit switches 331, power control system 34 drives slide 332 to move on guide rod 333, and by connecting two limit switches 331, the motion of slide 332 is controlled, when slide 332 is often close to a limit switch 331, proximity switch can send instruction to power control system 34, power control system 34 then drives slide 332 to move to another limit switch 331 place round about according to this instruction, and circulation is gone down successively.
Further, device provided by the present invention is utilized to have associating largely with receiving rod 41 and being preparing the quality of small-caliber artificial blood vessel, will be given below receiving several preferred implementations of rod 41, to help skilled in the art to understand technical scheme further and better to implement the present invention.
Refer to Fig. 2-1 and Fig. 2-2, Fig. 2-1 is a kind of embodiment principle schematic receiving rod, Fig. 2-2 receives rod complete section schematic diagram on vertical axis direction in Fig. 2-1, as shown in the figure, receiving the screw thread 412 on rod 41 is projection spiral bar 412a on cylindrical barred body 411 side, the helix of this spiral bar 412a stretches in the axial direction on described side, spiral bar 412a is at the outer convex arch that along the shape received on excellent 41 sections of vertical axis can be 120 °-180 °, it can be 1-6mm that spiral bar 412a place receives the outer diameter D of rod 41, spiral bar 412a height h on side can be 0.2-0.6mm, the helix pitch m of spiral bar 412a can be 2-6mm.
Concrete, the length receiving rod 41 could be arranged to L=60-120mm, receives rod 41 and adopts stainless steel preferably.
Referring back to Fig. 3-1 and Fig. 3-2, Fig. 3-1 is the another embodiment principle schematic receiving rod, Fig. 3-2 receives rod complete section schematic diagram on vertical axis direction in Fig. 3-1, as shown in the figure, it is recessed helicla flute 412b on cylindrical barred body 411 side at the screw thread received on rod 41, the helix of helicla flute 412b stretches in the axial direction on described side, helicla flute 412b is in the indent circular arc being shaped as 120 °-180 ° received on excellent 41 sections along vertical axis, it is 1-6mm that helicla flute 412b place receives the outer diameter D of rod 41, the degree of depth h of helicla flute 412b is 0.2-0.6mm, the helix pitch m of helicla flute 412b is 2-6mm.
Referring back to Fig. 4-1 and Fig. 4-2, Fig. 4-1 is another the embodiment principle schematic receiving rod, Fig. 4-2 receives rod complete section schematic diagram on vertical axis direction in Fig. 4-1, as shown in the figure, by recessed helicla flute 412b on cylindrical barred body 411 side at the screw thread received on rod 41 and twine the real silk 412c being located on helicla flute 412b and constitute, the helix of helicla flute 412b stretches in the axial direction on described side, it is 1-6mm that helicla flute 412b place receives the outer diameter D of rod 41, the degree of depth h of helicla flute 412b is 0.2-0.6mm, the helix pitch m of helicla flute 412b is 2-6mm.The present embodiment adopts the structure of helicla flute 412b and real silk 412c, its object is to: one, it is possible to play spacing effect by helicla flute 412b, and such real silk 412c can be more prone to when being wound on outside on excellent 41 sides of reception, in hgher efficiency；Its two, capillary matsurface can be formed in the thread surface that formed by real silk 412c, thus improving the percent of pass of artificial blood vessel.
In being embodied as, helicla flute 412b is in the indent circular arc being shaped as 120 °-180 ° received on excellent 41 sections along vertical axis, and real silk twines and is located in helicla flute 412b and forms bottom for having the helicla flute 412b of real silk.The artificial blood vessel that this structure prepares is better than Fig. 3-1 institute and receives the artificial blood vessel obtained by excellent 41 to structure.
In being embodied as, real silk 412c is wound around along helicla flute and is covered on helicla flute, is formed over the arc surface (i.e. structure shown in Fig. 4-2) protruding cylindrical barred body 411 side at helicla flute.Usually, the cambered surface angle of arc surface is 120 °-180 °.
Need exist for illustrate be, the reception rod 41 of above-mentioned three kinds of structures is provided to form helicla flute at artificial blood vessel's inwall, although being preferable to carry out in parameter can realize above-mentioned given, but there is also certain difference according to three kinds of effects implementing the obtained artificial blood vessel of structure, detailed difference is shown in table 1 below:
| Receive rod helicitic texture | Groove is high/deep | Helix pitch | Fluency (m/s) |
| Spiral bar | H=0.2-0.6mm | M=2-6mm | 3.6216±0.3281 |
| Helicla flute | H=0.2-0.6mm | M=2-6mm | 3.5893±0.4072 |
| Helicla flute+real silk | H=0.2-0.6mm | M=2-6mm | 3.8257±0.6294 |
Upper table is based on not obtained under identical parameters by the reception rod 41 of structure artificial blood vessels, and it is simulated, according to obtained artificial blood vessel, the test data that test draws, from result of the test, it is clear that it is the most efficient artificial blood vessel that the third helicla flute adds the structure of real silk.Its principle is in that, the circular arc helical structure moulded by real silk has small concaveconvex structure or perhaps capillary matsurface on surface, this is compared with the general helical surface with smooth surface prepared by metal material, the area of cell wall is more larger, it is clear that this is that common metal processing is done and cannot be realized.
The preparation process realizing artificial blood vessel by the small-caliber artificial blood vessel preparation facilities based on electrostatic spinning provided by the present invention is referred to following operating procedure and realizes:
The small-caliber artificial blood vessel preparation facilities that above-described embodiment provides can prepare effective band spiral fluted small-caliber artificial blood vessel so that band spiral fluted small-bore artificial blood vessel can realize preparation, provides possibility for its marketization.And the present invention gives the reception rod of multiple key structure, pass through the cooperation receiving other parts excellent and above-mentioned to implementing structure and realize the preparation with spiral fluted small-caliber artificial blood vessel well, and preparation-obtained vascular effects is more outstanding.
Embodiment 2
In addition, in the present embodiment, a kind of device provided by embodiment 1 is provided and realizes method prepared by artificial blood vessel, refer to Fig. 5, give a kind of device utilized in embodiment 1 to realize the flow chart of method prepared by artificial blood vessel, the step that realizes of the method will be elaborated below.
Step S10,0.4gL-lactic acid and epsilon-caprolactone copolymer (P (LLA-CL)) [full name is: Poly (L-lactide-co-epsilon-caprolactone)] is weighed with electronic balance, mol ratio is 50:50, it is dissolved in the hexafluoroisopropanol (linear formula: (CF3) 2CHOH) of 5ml, by magnetic stirrer vibration to dissolving completely, generally the rotating speed of magnetic stirring apparatus is set to 300rpm, obtains the electrostatic spinning solution that concentration is 8% (g/ml)；
Step S20, this electrostatic spinning solution is put in spinning solution injection device, by injected current amount control device therein, the fltting speed of fuse solution is set at about 1.0ml/h, regulate receiving range most about the 15cm received between rod and syringe needle, No. 8 syringe needles selected by syringe needle, and voltage high voltage power supply set is set to 12kv, obtain P (LLA-CL) nanofiber that average diameter is about 120nm；
Step S30, start injection slide unit control device medium power control system and the rotary power received in device controls device, reception rod is allowed to receive described P (LLA-CL) nanofiber, shape spinning carrying out artificial blood vessel, spinning carries out power-off after 4-8h, unload reception rod, from receiving, rod takes off the artificial blood vessel with inner spiral groove.
Above-mentioned the principle steps being only spinning artificial blood vessel to preparation method, and the artificial blood vessel obtaining better effects can be realized by preferred implementation once.
In being embodied as, when adopting same apparatus structure and spinning solution, adopting the reception rod of different structure, there is certain difference in its obtained artificial blood vessel's effect.
Such as, table 2 below is seen:
| Receive rod helicitic texture | Groove is high | Helix pitch | Fluency (m/s) |
| External spiral groove | H=0.2-0.6mm | M=2-6mm | 3.6416±0.3461 |
| Inner spiral groove | H=0.2-0.6mm | M=2-6mm | 3.5793±0.4352 |
| Inner spiral groove+real silk | H=0.2-0.6mm | M=2-6mm | 3.7957±0.6217 |
As can be seen from Table 2, when other condition is identical, adopting the reception rod of different structures, there is some difference for the effect of its artificial blood vessel obtained.Wherein, what effect was optimum should be reception rod that inner spiral groove adds real silk structure, and inner spiral groove and external spiral groove structure are taken second place successively.
In being embodied as, adopt the effect that artificial blood vessel also can be made by the preparation method of spinning solution to impact, see table 3 below:
Can be seen that from upper table 3 spinning solution is modulated at 6-12% can obtain good compliance, especially when 8-10%, it is best that its spinning formed prepares the compliance of artificial blood vessel.
Additionally, the Pfansteihl of present invention employing and epsilon-caprolactone copolymer P (LLA-CL) and hexafluoroisopropanol modulate spinning solution, it has better effect compared to existing spinning material.
A kind of Detailed operating procedures of during being embodied as by said apparatus being prepared artificial blood vessel is given below:
1) polymer solution being equipped with is proceeded in dimension syringe (during dosing, it is considered as tube for transfusion internal loss amount；If toxic solvent, need to wear masks and operate at fume hood), connect tube for transfusion and syringe needle, pushing syringe stops to liquid level in tube for transfusion to needle position, and residual polymer solution sealed membrane is sealed up for safekeeping；
2) high-voltage power apparatus and power control system and rotary power are controlled equipment safety ground connection；
3) high-voltage power apparatus, spinning solution injection device and power control system and rotary power are controlled device and connect power supply, open high-voltage power apparatus, spinning solution injection device and power control system and the on and off switch of rotary power control device successively；
4) artificial blood vessel is installed and receives rod, reception rod two ends ER16 and ER11 joint clamping are fixed on rotary power and control on device；
5) allotment injected current amount control device (i.e. syringe pump) parameter (includes syringe model, spinning capacity, the spinning time), can use in actual use and about controller, advance button adjustment syringe to lay space, mounting of syringe also uses draw-in groove clamping, regulate and advance button to make the complete clamping of syringe (first long press propelling, then press fine setting)；
6) the negative polarity connecting line of high-voltage power apparatus and syringe needle are connected；
7) regulate receiving range, reach best reception state；
8) regulating limit switch, control slide unit upper saddle and come and go position and distance, it is that 4-12cm arbitrarily regulates that limit switch can control the displacement range of slide, and concrete fixed position is determined according to required spinning length of vessel；
9) open power control unit to run on slide unit by slide, and regulate operating rate, wherein, the impact of slide unit shuttle speed size is little, as long as uniform motion, slide unit effect is so that time spinning blood vessel receives evenly, it is thus achieved that similar surface parameter；
10) regulate rotary power in reception device and control motor rotary speed in device, it is generally 100-600rpm, preferably can select about 300rpm, it is to be appreciated that it is more fast to receive rod rotating speed, reception is more good, obtain blood vessel more to compact, but can cause that porosity is more low, it is preferred that speed 100-600rpm, preferably can select about 300rpm；
11) open syringe pump and advance key；
12) press high voltage power supply and connect button in fact, after parameter stability, regulate voltage swing；
13) after spinning is stable, use slide to connect a small amount of spinning, examining under a microscope whether size meets spinning requirement, if not being inconsistent, can suitably regulate receiving range, voltage swing and fltting speed；
14) after spinning terminates, the power supply of each device or on and off switch in shutoff device, take off artificial blood vessel's receptor and artificial blood vessel taken out, arranging laboratory table.
In sum, the present invention is the preparation method that the preparation of small-caliber artificial blood vessel provides the device of realization, and give how to obtain more excellent artificial blood vessel be preferable to carry out device and method, thus making up prior art, at inwall, there is the blank in helical groove structure artificial blood vessel's preparation, there is substantial technical contribution and significant technique effect.
Claims (10)
1. the small-caliber artificial blood vessel preparation method based on electrostatic spinning, it is characterised in that described method is applied in following device: high-voltage power apparatus, includes the high voltage power supply of positive and negative polarity for exporting at least one road；
Spinning solution injection device, including for holding the injection device of spinning solution and exporting the injected current amount control device of spinning solution flow velocity size for regulation and control injection device, for accurately being exported according to the regulation and control of described injected current amount control device by the spinning solution in described injection device, the syringe needle on described injection device is also connected electrically in the positive polarity of the high voltage power supply that described high-voltage power apparatus exports；
Receive device, including for receiving receiving rod and supplying to control to receive the rotary power control device that rod rotates of electrostatic spinning, the described rod that receives realizes forward or reverse motion by being connected on described rotary power control device, and it is connected electrically in the negative polarity of the high voltage power supply that described high-voltage power apparatus exports, wherein, described reception rod is conductor and its cylindrically shaped barred body, and described cylindrical bar body side surface is provided with screw thread；
Injection slide unit controls device, it is arranged on described reception device side, for regulating described syringe needle on described injection device and described reception interrod spacing and angle of inclination, to set up between rod for spinning solution by forming the high-voltage electrostatic field of nanometer filamentary fibers at syringe needle and receiving, and it is used for controlling described syringe needle and controls device at described injection slide unit and move reciprocatingly；
The step that realizes of described method includes:
Weighing Pfansteihl and epsilon-caprolactone copolymer, be dissolved in hexafluoroisopropanol, stirring vibration, to dissolving completely, obtains the electrostatic spinning solution that concentration is 6-12%；
This electrostatic spinning solution is put in spinning solution injection device, by injected current amount control device therein, the fltting speed of fuse solution is set at 0.8-2.0ml/h, regulate the receiving range most 10-20cm received between rod and syringe needle, select suitable syringe needle, and voltage high voltage power supply set is set to 8-15kv；
Start injection slide unit control device medium power control system and the rotary power received in device controls device, allowing reception rod receive described nanofiber, shape spinning carrying out artificial blood vessel, spinning carries out power-off after 4-8h, unload reception rod, from receiving, rod takes off the artificial blood vessel with inner spiral groove.
2. the small-caliber artificial blood vessel preparation method based on electrostatic spinning according to claim 1, it is characterised in that described injection slide unit controls device and at least includes:
Swing arm；
Porous connecting row support, is movably connected on one end of swing arm, including multiple through holes side by side, for the described syringe needle of different model is individually fixed in the plurality of through hole side by side；
Slide unit, including two spaced limit switches with at the reciprocating slide in this interval, described slide is fixedly connected on the other end of swing arm；
Power control system, is connected to described slide and limit switch, for receiving the close signal of slide that any of which limit switch sends to drive described slide to move to another limit switch direction.
3. the small-caliber artificial blood vessel preparation method based on electrostatic spinning according to claim 1, it is characterised in that: described injection device is syringe, and described injected current amount control device is syringe pump.
4. the small-caliber artificial blood vessel preparation method based on electrostatic spinning according to claim 1, it is characterized in that: described rotary power controls device and is at least made up of motor and fixing seat, interval is just arranged by described fixing seat and motor, the rotating shaft of described motor is achieved a fixed connection by chuck with described one end receiving rod, and the described other end receiving rod is movably connected on described fixing seat.
5. the small-caliber artificial blood vessel preparation method based on electrostatic spinning according to claim 1, it is characterized in that: described screw thread is projection spiral bar on cylindrical bar body side surface, the helix of described spiral bar stretches in the axial direction on described side, spiral bar is at the outer convex arch being shaped as 120 °-180 ° received on rod section along vertical axis, it is 1-6mm that described spiral bar place receives the outer diameter D of rod, described spiral bar height h on side is 0.2-0.6mm, and the helix pitch m of described spiral bar is 2-6mm.
6. the small-caliber artificial blood vessel preparation method based on electrostatic spinning according to claim 1, it is characterized in that: described screw thread is recessed helicla flute on cylindrical bar body side surface, spiral fluted helix stretches in the axial direction on described side, described helicla flute is in the indent circular arc being shaped as 120 °-180 ° received on rod section along vertical axis, it is 1-6mm that described helicla flute place receives the outer diameter D of rod, described spiral fluted degree of depth h is 0.2-0.6mm, and described spiral fluted helix pitch m is 2-6mm.
7. the small-caliber artificial blood vessel preparation method based on electrostatic spinning according to claim 1, it is characterized in that: described screw thread is by recessed helicla flute on cylindrical bar body side surface and twines the real silk being located on helicla flute and constitutes, described spiral fluted helix stretches in the axial direction on described side, it is 1-6mm that described helicla flute place receives the outer diameter D of rod, described spiral fluted degree of depth h is 0.2-0.6mm, and described spiral fluted helix pitch m is 2-6mm.
8. the small-caliber artificial blood vessel preparation method based on electrostatic spinning according to claim 7, it is characterized in that: described helicla flute is in the indent circular arc being shaped as 120 °-180 ° received on rod section along vertical axis, and described real silk twines and is located in helicla flute and forms bottom for having the helicla flute of real silk.
9. the small-caliber artificial blood vessel preparation method based on electrostatic spinning according to claim 7, it is characterised in that: described real silk is wound around along helicla flute and is covered on described helicla flute, is formed over the arc surface protruding cylindrical bar body side surface at described helicla flute.
10. the small-caliber artificial blood vessel preparation method based on electrostatic spinning according to claim 9, it is characterised in that: the cambered surface angle of described arc surface is 120 °-180 °.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642719A (en) * | 2018-05-11 | 2018-10-12 | 西安工程大学 | A kind of apparatus and method of Static Spinning integration small-caliber artificial blood vessel holder |
| CN110481019A (en) * | 2019-08-20 | 2019-11-22 | 广州帷幄生物科技有限公司 | A kind of artificial blood vessel printing device |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006129910A1 (en) * | 2005-05-02 | 2006-12-07 | Industrial Cooperation Foundation Chonbuk National University | Procede de fabrication d’un filament continu par electro-filage et filament continu fabrique de cette maniere |
| CN101708344A (en) * | 2009-11-27 | 2010-05-19 | 天津大学 | Nanofiber vascular prostheses and preparation method |
| CN101785875A (en) * | 2010-02-25 | 2010-07-28 | 王深明 | Preparation method of nano superfine fiber vascular prosthesis |
| CN101785714A (en) * | 2010-02-25 | 2010-07-28 | 王深明 | Electric spinning device for preparing artificial blood vessel |
| CN102266255A (en) * | 2011-05-17 | 2011-12-07 | 东华大学 | Conical corrugated small-caliber artificial blood vessel as well as electrostatic spinning production method and equipment thereof |
| CN104841013A (en) * | 2015-05-04 | 2015-08-19 | 东华大学 | Composite nanofiber/nano yarn double-layer intravascular stent and preparation method thereof |
| CN105079874A (en) * | 2014-05-14 | 2015-11-25 | 复旦大学附属华山医院 | Method for preparing small-diameter artificial blood vessels on basis of nanotechnologies |
| CN204982155U (en) * | 2015-07-31 | 2016-01-20 | 佛山轻子精密测控技术有限公司 | Batch manufacture nanofiber's electrostatic spinning device and system |
| CN105310796A (en) * | 2014-06-26 | 2016-02-10 | 上海微创医疗器械（集团）有限公司 | Artificial blood vessel and preparation method thereof and needling die |
-
2016
- 2016-04-01 CN CN201610200049.0A patent/CN105755554B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006129910A1 (en) * | 2005-05-02 | 2006-12-07 | Industrial Cooperation Foundation Chonbuk National University | Procede de fabrication d’un filament continu par electro-filage et filament continu fabrique de cette maniere |
| CN101708344A (en) * | 2009-11-27 | 2010-05-19 | 天津大学 | Nanofiber vascular prostheses and preparation method |
| CN101785875A (en) * | 2010-02-25 | 2010-07-28 | 王深明 | Preparation method of nano superfine fiber vascular prosthesis |
| CN101785714A (en) * | 2010-02-25 | 2010-07-28 | 王深明 | Electric spinning device for preparing artificial blood vessel |
| CN102266255A (en) * | 2011-05-17 | 2011-12-07 | 东华大学 | Conical corrugated small-caliber artificial blood vessel as well as electrostatic spinning production method and equipment thereof |
| CN105079874A (en) * | 2014-05-14 | 2015-11-25 | 复旦大学附属华山医院 | Method for preparing small-diameter artificial blood vessels on basis of nanotechnologies |
| CN105310796A (en) * | 2014-06-26 | 2016-02-10 | 上海微创医疗器械（集团）有限公司 | Artificial blood vessel and preparation method thereof and needling die |
| CN104841013A (en) * | 2015-05-04 | 2015-08-19 | 东华大学 | Composite nanofiber/nano yarn double-layer intravascular stent and preparation method thereof |
| CN204982155U (en) * | 2015-07-31 | 2016-01-20 | 佛山轻子精密测控技术有限公司 | Batch manufacture nanofiber's electrostatic spinning device and system |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642719A (en) * | 2018-05-11 | 2018-10-12 | 西安工程大学 | A kind of apparatus and method of Static Spinning integration small-caliber artificial blood vessel holder |
| CN108642719B (en) * | 2018-05-11 | 2020-12-01 | 西安工程大学 | Preparation method of integrated small-caliber artificial blood vessel stent |
| CN110481019A (en) * | 2019-08-20 | 2019-11-22 | 广州帷幄生物科技有限公司 | A kind of artificial blood vessel printing device |
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