CN108634311A - A kind of process of extraction tealeaves active material - Google Patents
A kind of process of extraction tealeaves active material Download PDFInfo
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- CN108634311A CN108634311A CN201810431314.5A CN201810431314A CN108634311A CN 108634311 A CN108634311 A CN 108634311A CN 201810431314 A CN201810431314 A CN 201810431314A CN 108634311 A CN108634311 A CN 108634311A
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- 238000000605 extraction Methods 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 30
- 239000011149 active material Substances 0.000 title claims abstract description 23
- 230000008569 process Effects 0.000 title claims abstract description 22
- 241001122767 Theaceae Species 0.000 claims abstract description 44
- DATAGRPVKZEWHA-YFKPBYRVSA-N N(5)-ethyl-L-glutamine Chemical compound CCNC(=O)CC[C@H]([NH3+])C([O-])=O DATAGRPVKZEWHA-YFKPBYRVSA-N 0.000 claims abstract description 22
- 150000004676 glycans Chemical class 0.000 claims abstract description 22
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 22
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 22
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 22
- 239000005017 polysaccharide Substances 0.000 claims abstract description 22
- 239000000284 extract Substances 0.000 claims abstract description 16
- 238000000926 separation method Methods 0.000 claims abstract description 15
- 239000011347 resin Substances 0.000 claims abstract description 14
- 229920005989 resin Polymers 0.000 claims abstract description 14
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229940026510 theanine Drugs 0.000 claims abstract description 11
- 239000002904 solvent Substances 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 36
- 239000012530 fluid Substances 0.000 claims description 16
- 238000001914 filtration Methods 0.000 claims description 15
- 239000012466 permeate Substances 0.000 claims description 15
- 239000000243 solution Substances 0.000 claims description 12
- 235000019441 ethanol Nutrition 0.000 claims description 11
- 239000003729 cation exchange resin Substances 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 10
- 239000012528 membrane Substances 0.000 claims description 10
- 238000001556 precipitation Methods 0.000 claims description 10
- 238000000108 ultra-filtration Methods 0.000 claims description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 9
- 239000002253 acid Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 8
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 8
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 7
- 239000000908 ammonium hydroxide Substances 0.000 claims description 7
- 150000001768 cations Chemical class 0.000 claims description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 6
- 230000002378 acidificating effect Effects 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 238000002425 crystallisation Methods 0.000 claims description 5
- 230000008025 crystallization Effects 0.000 claims description 5
- 238000004090 dissolution Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 238000010828 elution Methods 0.000 claims description 5
- 238000005342 ion exchange Methods 0.000 claims description 5
- 238000001223 reverse osmosis Methods 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 238000001291 vacuum drying Methods 0.000 claims description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 claims description 4
- 230000002255 enzymatic effect Effects 0.000 claims description 4
- 238000002955 isolation Methods 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 3
- 238000005341 cation exchange Methods 0.000 claims description 3
- 230000008901 benefit Effects 0.000 abstract description 4
- 239000003456 ion exchange resin Substances 0.000 abstract description 3
- 229920003303 ion-exchange polymer Polymers 0.000 abstract description 3
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 230000001603 reducing effect Effects 0.000 description 4
- 102000035195 Peptidases Human genes 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002218 hypoglycaemic effect Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 230000004224 protection Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 101710138460 Leaf protein Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 230000001631 hypertensive effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229920006008 lipopolysaccharide Polymers 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000002936 tranquilizing effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/163—Liquid or semi-liquid tea extract preparations, e.g. gels, liquid extracts in solid capsules
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/166—Addition of, or treatment with, enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/18—Extraction of water soluble tea constituents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Botany (AREA)
- Dispersion Chemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of processes of extraction tealeaves active material, belong to tea product technical field.A kind of process of extraction tealeaves active material of the present invention is using water as solvent, using the active ingredient in ultrasonic wave extraction tealeaves, has many advantages, such as that extraction time is short, efficient, active material loss is few；Using three kinds of ion exchange resin separation theanine, tea polyphenols and tea polysaccharide active materials, resin material is reusable, has higher cost performance；Obtained extract purity is high, tea polysaccharide total sugar content >=72%；Purity >=99% of tea polyphenols；Purity >=85% of theanine.
Description
Technical field
The invention belongs to tea product technical fields, and in particular to a kind of process of extraction tealeaves active material.
Background technology
The ingredient of 19 beginnings of the century, tealeaves just gradually clearly get up.By the separation and identification of modern science, contain in tealeaves
Chemical machine ingredient is up to more than 450 kinds, and inorganic mineral element is up to more than 40 kinds.Organic chemical composition mainly has：Tea polyphenols,
Vegetable soda, protein, amino acid, vitamin, pectin element, organic acid, lipopolysaccharides, carbohydrate, enzyme, pigment etc..Inorganic mineral member
Element mainly has：Potassium, calcium, magnesium, cobalt, iron, aluminium, sodium, zinc, copper, nitrogen, phosphorus, fluorine, iodine, selenium etc..
With tealeaves research deepen continuously and with the intersection of medical research and infiltration, it has already been proven that theanine has anti-
Tumour, promotes the medical functions such as nerve growth at hypertensive tranquilizing；Tea polyphenols have different physiological roles, it is not only good natural
Antioxidant also has enhancing Abwehrkraft des Koepers, anti-cancer, antitumor, radioresistance, antibacterial, antiviral, hypoglycemic, reducing blood lipid, pre-
Anti- angiocardiopathy, anti-aging, prevent skin it is ultraviolet damage etc. different physiological roles, grease, food, medicine, daily-use chemical industry,
The fields such as light industry have broad prospect of application；Tea polysaccharide have a variety of physiological actions, as radiation protection, anticoagulation and antithrombotic,
Hypoglycemic etc., it can also enhance body's immunity, experiments have shown that tea polysaccharide has blood pressure lowering, reducing heart rate, resist oxygen lack, increasing
It the effects that adding coronary flow, can be with reducing blood lipid, anti arteriosclerosis, anti-inflammatory and anticancer etc..Therefore, safely and effectively from tea
The ingredients such as theanine, tea polyphenols and tea polysaccharide are extracted and detached in leaf, and there is important practical significance.
That there are recovery rates is relatively low for the extracting mode of current tealeaves active material, solvent dosage is big, effective component extracts
The disadvantages such as purity is low.
Invention content
In view of the above-mentioned problems, the present invention is based on ultrasonic wave extraction, ultrafiltration membrane technique and ion-exchange resin techniques to carry
For a kind of process of energy-efficient extraction tealeaves active material.
In order to achieve the above objectives, technical solution proposed by the invention is：A kind of technique side of extraction tealeaves active material
Method, it is characterised in that：Include the following steps：
1) 10-30 times of water is added in tea-leaf power, under ul-trasonic irradiation, in 60 DEG C of -80 DEG C of temperature ranges, every time
15-30 minutes, soak extraction 3-5 times repeatedly was separated by filtration to obtain tea extract；
2) tea extract is through being concentrated in vacuo, by concentrate and ethanol solution according to 1:3-1:5 volume ratios are uniformly mixed
Merge stirring, polysaccharide precipitation and solution are respectively obtained after filtering；By polysaccharide precipitation water dissolution, pH value 1- is utilized after removing deproteinized
3 strong-acid cation-exchange resin is adsorbed and is detached, and obtains tea polysaccharide；
3) it by solution after Polyose extraction, adjusts pH3.5-5 to obtain film permeate and trapped fluid through ultrafiltration membrance filter with acid, cuts
Stay liquid that filtrate and protein feed solid content is obtained by filtration through centrifuge；Filtrate is merged with film permeate, is then handed over cation
It changes resin to extract, obtains the resin for being adsorbed with tea polyphenols and acidic aqueous solution；The cation for being adsorbed with tea polyphenols is exchanged
Resin is added in absolute ethyl alcohol, is detached under being mixed slowly in 50-60 DEG C, by separation of ethanol liquid reverse osmosis membrane separation with it is dense
Contracting, obtains film permeate and trapped fluid, trapped fluid obtains the distillate containing ethyl alcohol and tea polyphenols through low-temperature vacuum drying；
4) by distillate, adjustment pH value is 1-5, then passes through cation exchange resin column and carries out ion exchange, with 10%
Concentration ammonium hydroxide elutes, and collects efflux, concentration, and theanine can be obtained in cooling, crystallization, drying.
Further scheme,
Separation solvent for use described in step 2 is any one in absolute ethyl alcohol, acetic acid, acetone.
Further scheme,
It is any one in hydrochloric acid, sulfuric acid and phosphoric acid that acid used in pH value is adjusted in the step 3.
Further scheme,
Ultrafiltration membrane in the step 3 selects aperture O.8-5 micron capillary tubular type ultrafiltration membrane, operating pressure 1.2-
1.6Mpa。
Further scheme,
Efflux in step 4 by ammonium hydroxide elution is concentrated into 1/3-1/5.
Further scheme,
Described in step 2 except deproteinized method be basic protein enzymatic isolation method, basic protein enzyme amount used be 1600-
2000U/g。
Further scheme,
The enzymatic isolation method process conditions are：PH value 9-10,25-30 DEG C of temperature, time 45-75min.
Advantageous effect：
1) a kind of process of extraction tealeaves active material of the invention is using water as solvent, using ultrasonic wave extraction tealeaves
In active ingredient, have many advantages, such as that extraction time is short, the loss of efficient, active material is few；
2) a kind of process of extraction tealeaves active material of the invention is using ion exchange resin separation theanine, tea
Three kinds of active materials of polyphenol and tea polysaccharide, resin material is reusable, has higher cost performance；
3) a kind of process of extraction tealeaves active material of the invention removes tea leaf protein with enzymatic isolation method, has efficient
The advantage of environmental protection；
4) extract purity that a kind of process of extraction tealeaves active material of the invention obtains is high, tea polysaccharide total reducing sugar
Content >=72%；Purity >=99% of tea polyphenols；Purity >=85% of theanine.
Specific implementation mode
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, below to the specific reality of the present invention
The mode of applying elaborates.Many details are elaborated in the following description in order to fully understand the present invention.But this
Invention can be much to implement different from other manner described here, and those skilled in the art can be without prejudice to the present invention
Similar improvement is done in the case of intension, therefore the present invention is not limited by following public specific implementation mode.
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1
1) 10 times of water is added in tea-leaf power, under ul-trasonic irradiation, in 80 DEG C of temperature ranges, 15 minutes every time, instead
Multiple soak extraction 5 times, is separated by filtration to obtain tea extract；
2) tea extract is through being concentrated in vacuo, by concentrate and absolute ethyl alcohol according to 1:3 volume ratios are uniformly mixed and stirred for,
Polysaccharide precipitation and solution are respectively obtained after filtering；By polysaccharide precipitation water dissolution；With 1600U/g alkali proteases, process conditions
For pH value 10,25 DEG C of temperature, the time, 45min removed removing protein；It adsorbs and detaches using the strong-acid cation-exchange resin of pH value 1,
Obtain tea polysaccharide；
3) by solution after Polyose extraction, film permeate and trapped fluid are obtained through ultrafiltration membrance filter with sulfuric acid tune pH3.5, is cut
Stay liquid that filtrate and protein feed solid content is obtained by filtration through centrifuge；Filtrate is merged with film permeate, is then handed over cation
It changes resin to extract, obtains the resin for being adsorbed with tea polyphenols and acidic aqueous solution；The cation for being adsorbed with tea polyphenols is exchanged
Resin is added in absolute ethyl alcohol, is detached under being mixed slowly in 50 DEG C, by separation of ethanol liquid reverse osmosis membrane separation and is concentrated,
Film permeate and trapped fluid are obtained, trapped fluid obtains the distillate containing ethyl alcohol and tea polyphenols through low-temperature vacuum drying；
4) by distillate, adjustment pH value is 1, then passes through cation exchange resin column and carries out ion exchange, dense with 10%
Ammonium hydroxide elution is spent, efflux, concentration are collected, theanine can be obtained in cooling, crystallization, drying.
Embodiment 2
1) 30 times of water is added in tea-leaf power, under ul-trasonic irradiation, in 60 DEG C of temperature ranges, 30 minutes every time, instead
Multiple soak extraction 3 times, is separated by filtration to obtain tea extract；
2) tea extract is through being concentrated in vacuo, by concentrate and acetic acid according to 1:5 volume ratios are uniformly mixed and stirred for, filtering
After respectively obtain polysaccharide precipitation and solution；By polysaccharide precipitation water dissolution；With 2000U/g alkali proteases, process conditions PH
Value 9,30 DEG C of temperature, the time, 75min removed removing protein；It adsorbs and detaches using the strong-acid cation-exchange resin of pH value 3, obtain tea
Polysaccharide；
3) by solution after Polyose extraction, film permeate and trapped fluid are obtained through ultrafiltration membrance filter with phosphoric acid tune pH5, is retained
Filtrate and protein feed solid content is obtained by filtration through centrifuge in liquid；Filtrate is merged with film permeate, is then exchanged with cation
Resin extracts, and obtains the resin for being adsorbed with tea polyphenols and acidic aqueous solution；The cation exchange tree of tea polyphenols will be adsorbed with
Fat is added in absolute ethyl alcohol, is detached under being mixed slowly in 60 DEG C, and separation of ethanol liquid reverse osmosis membrane separation and concentration obtain
To film permeate and trapped fluid, trapped fluid obtains the distillate containing ethyl alcohol and tea polyphenols through low-temperature vacuum drying；
4) by distillate, adjustment pH value is 5, then passes through cation exchange resin column and carries out ion exchange, dense with 10%
Ammonium hydroxide elution is spent, efflux, concentration are collected, theanine can be obtained in cooling, crystallization, drying.
Embodiment 3
1) 20 times of water is added in tea-leaf power, under ul-trasonic irradiation, in 70 DEG C of temperature ranges, 25 minutes every time, instead
Multiple soak extraction 4 times, is separated by filtration to obtain tea extract；
2) tea extract is through being concentrated in vacuo, by concentrate and acetone according to 1:4 volume ratios are uniformly mixed and stirred for, filtering
After respectively obtain polysaccharide precipitation and solution；By polysaccharide precipitation water dissolution；With 1800U/g alkali proteases, process conditions PH
Value 9.5,28 DEG C of temperature, the time, 60min removed removing protein；It adsorbs and detaches using the strong-acid cation-exchange resin of pH value 2, obtain
Tea polysaccharide；
3) by solution after Polyose extraction, film permeate and trapped fluid are obtained through ultrafiltration membrance filter with sulfuric acid tune pH4, is retained
Filtrate and protein feed solid content is obtained by filtration through centrifuge in liquid；Filtrate is merged with film permeate, is then exchanged with cation
Resin extracts, and obtains the resin for being adsorbed with tea polyphenols and acidic aqueous solution；The cation exchange tree of tea polyphenols will be adsorbed with
Fat is added in absolute ethyl alcohol, is detached under being mixed slowly in 55 DEG C, and separation of ethanol liquid reverse osmosis membrane separation and concentration obtain
To film permeate and trapped fluid, trapped fluid obtains the distillate containing ethyl alcohol and tea polyphenols through low-temperature vacuum drying；
4) by distillate, adjustment pH value is 3, then passes through cation exchange resin column and carries out ion exchange, dense with 10%
Ammonium hydroxide elution is spent, efflux, concentration are collected, theanine can be obtained in cooling, crystallization, drying.
Although hereinbefore having been made with reference to some embodiments, present invention is described, do not departing from the present invention's
In the case of range, it can be carried out various improvement and can use it is equivalent without replacement technology point therein, especially, as long as
There is no technical contradiction, the various features in the various embodiments of institute's careless mistake of the present invention can be combined by either type and be made
It is only in omitting length and economize on resources with, the description that the case where not combined in the present invention to these carries out exhaustive row
Consider.Therefore, the invention is not limited in specific embodiments disclosed herein, and include falling into claim.
Claims (7)
1. a kind of process of extraction tealeaves active material, is crushed to 30-50 mesh by tealeaves first, it is characterised in that：Method
The step of it is as follows：
1) 10-30 times of water is added in tea-leaf power, under ul-trasonic irradiation, in 60 DEG C of -80 DEG C of temperature ranges, each 15-30
Minute, soak extraction 3-5 times repeatedly is separated by filtration to obtain tea extract；
2) tea extract is through being concentrated in vacuo, by concentrate and solvent according to 1:3-1:5 volume ratios are uniformly mixed and stirred for, filtering
After respectively obtain polysaccharide precipitation and solution；By polysaccharide precipitation water dissolution, except the strong-acid cation for utilizing pH value 1-3 after deproteinized
Exchanger resin is adsorbed and is detached, and obtains tea polysaccharide；
3) by solution after Polyose extraction, pH3.5-5 is adjusted to obtain film permeate and trapped fluid, trapped fluid through ultrafiltration membrance filter with acid
Filtrate and protein feed solid content is obtained by filtration through centrifuge；Filtrate is merged with film permeate, then uses cation exchange tree
Fat extracts, and obtains the resin for being adsorbed with tea polyphenols and acidic aqueous solution；The cation exchange resin of tea polyphenols will be adsorbed with
It is added in absolute ethyl alcohol, is detached under being mixed slowly in 50-60 DEG C, by separation of ethanol liquid reverse osmosis membrane separation and concentrated,
Film permeate and trapped fluid are obtained, trapped fluid obtains the distillate containing ethyl alcohol and tea polyphenols through low-temperature vacuum drying；
4) by distillate, adjustment pH value is 1-5, then passes through cation exchange resin column and carries out ion exchange, with 10% concentration
Ammonium hydroxide elutes, and collects efflux, concentration, and theanine can be obtained in cooling, crystallization, drying.
2. a kind of process of extraction tealeaves active material according to claim 1, it is characterised in that：Institute in step 2
The separation solvent for use stated is any one in absolute ethyl alcohol, acetic acid, acetone.
3. a kind of process of extraction tealeaves active material according to claim 1, it is characterised in that：The step 3
Acid used in middle adjusting pH value is any one in hydrochloric acid, sulfuric acid and phosphoric acid.
4. a kind of process of extraction tealeaves active material according to claim 1, it is characterised in that：The step 3
In ultrafiltration membrane select aperture O.8-5 micron capillary tubular type ultrafiltration membrane, operating pressure 1.2-1.6Mpa.
5. a kind of process of extraction tealeaves active material according to claim 1, it is characterised in that：The step 4
The middle efflux by ammonium hydroxide elution is concentrated into 1/3-1/5.
6. a kind of process of extraction tealeaves active material according to claim 1, it is characterised in that：The removing
The method of albumen is basic protein enzymatic isolation method, and basic protein enzyme amount used is 1600-2000U/g.
7. a kind of process of extraction tealeaves active material according to claim 6, it is characterised in that：The enzymolysis
Method process conditions are：PH value 9-10,25-30 DEG C of temperature, time 45-75min.
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| CN113845991A (en) * | 2021-10-20 | 2021-12-28 | 泸州老窖股份有限公司 | Tea wine and preparation method thereof |
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