CN108634117A - A method for the treatment of bamboo rat diarrhea using caprophyl implantation technique - Google Patents
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Abstract
The present invention relates to bamboo rat epidemic disease treatment technology fields,Especially a kind of method for treating bamboo rat diarrhea using caprophyl implantation technique,Using source of the female bamboo rat as caprophyl of growing up,Using maize straw,Hybrid giant napier bar,Bamboo root,Watermelon peel,Sweet potato,Peanut,The continuous feeding of soya bean,Bacterial community in the female bamboo rat enteron aisle of conditioning adult and number,Caprophyl to treat diarrhea bamboo rat provides stabilization,Good flora,Timely collection is taken in vitro excrement,The mode fermented with sodium citrate and in short-term screens the flora in excrement,Flora in extraction separating feces obtains caprophyl liquid,By caprophyl liquid and carrot,Bamboo branch,Niblet,Mung bean,Peanut,Radix Angelicae Sinensis,Palm,Jasmine is mixed,Bamboo rat to suffering from diarrhea feeds,Be conducive to the digestion of illness bamboo rat,Promote bamboo rat enteron aisle body recovery,Its function of intestinal canal restores normal after healing,Weight gain is rapid,Reduce the economic loss brought by bamboo rat diarrhea.
Description
Technical field
The present invention relates to bamboo rat epidemic disease treatment technology fields, especially a kind of to treat bamboo rat diarrhea using caprophyl implantation technique
Method.
Background technology
Caprophyl transplanting refers to migrating to the functional flora in healthy individuals excrement in the gastrointestinal tract of patient, and rebuilding has just
The intestinal flora of Chang Gongneng, the method for treating disease inside and outside gastrointestinal tract.First Application caprophyl transplantation treatment disease can trace back to
Eastern Jin Dynasty's epoch before 1700, Ge Hong cross first emergency medicine books at me《Anxious standby side after elbow》Described in：It treats in food
The patient that poison, diarrhea, fever are at death's door, " one liter of drink excrement juice, i.e., living ".Scientific investigations showed that, species difference, growth are sent out at present
Educate, the factors such as eating habit can all influence the structure, number of intestinal flora in animal body, and healthy individuals and diseased individuals it
Between intestinal flora it is also different.Then, the intestinal flora that sufferer individual is rebuild using the intestinal flora of healthy individuals is just gradual
As modern medicine, the research hotspot of biology.
Bamboo rat (scientific name：Rhizomyidae), also known as Zhu Chi, bamboo root mouse, awns leopard cat etc., gain the name because mainly searching for food bamboo.Mesh
The preceding whole world finds there is 6 kinds of 3 category altogether, wherein the domestic kind in China be Rhizomys sinesis, Rhizomys sumatrensis (Roffles) and hoarybamboo rat, it is widely distributed
In China Guangxi, Guizhou, Yunnan.Bamboo rat has very high economic value, and meat exquisiteness is soft, full of nutrition, not only has
The features such as low cholesterol, low fat, high protein, is also rich in calcium, iron, zinc, phosphorus, vitamin E and a variety of amino acid.According to《This
Careless detailed outline》It records：" bamboo rat meat is sweet, flat, nontoxic, tonifying middle-Jiao and Qi, deciphering ", at the same also have " menstruation regulating, defend the doctrine, enriching the blood and tonifying qi, taste
The characteristics of cloudy establishing-Yang ".
During bamboo rat is propagated artificially, due to feed, environmental sanitation, ventilation, temperature etc., bamboo can be caused
Mouse enteric infection, internal flora is unbalance, and then diarrhea, the symptoms such as have loose bowels occur so that breeding process cost increases, and directly affects
The economic benefit of bamboo rat cultivation.In the modern times cultivate, generally use Enrofloxacin, cefotaxime, Norfloxacin are to bamboo rat abdomen
It rushes down and is treated, but in use, the same dose of chemicals are used because the degree of bamboo rat itself symptom is different,
It is easy to cause the bamboo rat own digestive system decline after treatment end, body weight growth rate declines.Such as
A kind of Chinese medicine composition for treating bamboo rat diarrhea and dysentery disclosed in CN201310632906.0, using the fruit of Cherokee rose, guava, big
Leafiness, bigleafbeautyberry root or leaf, Rhizoma Atractylodis Macrocephalae, Radix Glycyrrhizae, medicament obtained by mixing or decoct after each Chinese medicine material being crushed respectively, in antidiarrheal stop dysentery
While, also have the function of swelling and pain relieving, controlling of bleeding with astringents, repair wound and promotes cellular immunity.But in actual production process
In, Chinese medicine effect slowly effect.
Invention content
In order to solve above-mentioned problem of the prior art, bamboo rat abdomen is treated using caprophyl implantation technique the present invention provides a kind of
The method rushed down.
It is achieved particular by following technical scheme：
A method of bamboo rat diarrhea being treated using caprophyl implantation technique, it is mixed using the bamboo rat excrement for not suffering from symptom of diarrhea
Enter in feed, feeding suffers from the bamboo rat of symptom of diarrhea.
Further, the bamboo rat for not suffering from symptom of diarrhea is the female bamboo rat that grows up.
Further, the bamboo rat excrement is the natural row that bamboo rat continuously feeds conditioning-type feed gained after 25~30 days
Let out object.
Further, the conditioning-type feed includes following component in parts by mass：
35~60 parts of maize straw, 20~24 parts of hybrid giant napier bar, 15~18 parts of bamboo root, 8~10 parts of watermelon peel, sweet potato 5~9
Part, 6~9 parts of peanut, 3~6 parts of soya bean.
Further, the bamboo rat excrement is mixed into feed, includes the following steps：
(1) sodium citrate solution of 0.3~0.7wt.%, bamboo rat excrement and citric acid are sprayed to the bamboo rat excrement after in vitro
The mass ratio of sodium solution is 10:1,5min is stirred, ferment 3~5h under 34~37 DEG C of aseptic condition, obtains fermentation excrement；
(2) fermentation caprophyl is diluted to 5 times with physiological saline, is ultrasonically treated, ultrasonic time is 1~3min, frequency
For 100~150Hz, then with the screen filtration of 800~1000 mesh, fermentation caprophyl liquid is obtained；
(3) fermentation caprophyl liquid sprays feed, stirs 1~3min, you can.
Further, the proportioning of fermentation caprophyl liquid and feed is 4~6ml：200g.
Further, the feed, including following preparation process：
20~25 portions of carrots, 18~22 parts of bamboo branch are chopped into 2~3cm, with 8~10 parts of niblets, 7~9 parts of mung beans, 2
~4 portions of peanuts are uniformly mixed.
Further, the feed is also added with 0.1~0.3 part of Radix Angelicae Sinensis, 0.2 portion of palm, 0.1 part of Jasmine.
Compared with prior art, the present invention has the following advantages：
The present invention using the source of female bamboo rat as caprophyl of growing up, using maize straw, hybrid giant napier bar, bamboo root, watermelon peel,
The continuous feeding of sweet potato, peanut, soya bean 25~30 days improves the Bacterial community and number grown up in female bamboo rat enteron aisle, to treat abdomen
The caprophyl for rushing down bamboo rat provides stable, good flora, timely collection is taken in vitro excrement, with sodium citrate and short time
The mode of fermentation screens the flora in excrement, and the flora extracted in separating feces obtains caprophyl liquid, by caprophyl liquid and Hu
Radish, bamboo branch, niblet, mung bean, peanut, Radix Angelicae Sinensis, palm, Jasmine are mixed, and the bamboo rat to suffering from diarrhea feeds, institute
The feed and its active ingredient of feeding are conducive to the digestion of illness bamboo rat, promote bamboo rat enteron aisle body recovery, after healing its enteron aisle work(
It can restore normal, weight gain is rapid, reduces the economic loss brought by bamboo rat diarrhea.
The present invention relative to use chemotherapy bamboo rat diarrhea, the bamboo rat cured after healing, intestinal tract by
Damage degree is small, the fast quick-recovery body weight increase of energy, and recovery effects are good；Relative to traditional Chinese medicine therapeutic modality, average responding time
And average cured time is shorter, there is preferable therapeutic effect, effective percentage can reach 85%~90% bamboo rat diarrhea.
Specific implementation mode
Further solution is made to the technical solution of the invention with reference to specific embodiment and experimental example
It releases and illustrates, in order to which those skilled in the art fully understand the invention, but the explanation and illustration, it is not to this
The further restriction of innovation and creation technical solution, on the basis of the innovation and creation, the replacement for the simple numerical value made and
The improved technical solution of general adjustment, belongs to the protection domain of the invention.
Embodiment 1
Choose the female bamboo rat of adult for not suffering from symptom of diarrhea, healthy growth, continuous 25 days feeding conditioning-type feeds, conditioning-type
Feed is maize straw 35g, hybrid giant napier bar 20g, bamboo root 15g, watermelon peel 8g, sweet potato 5g, peanut 6g, soya bean 3g；After 25 days,
The excrement that bamboo rat drains naturally is collected immediately after in vitro；The excrement of collection and the sodium citrate solution of 0.3wt.% are pressed
Mass ratio 10:1 mixing, stirs 5min, and ferment 5h under 34 DEG C of aseptic condition, then is diluted to fermentation caprophyl with physiological saline
It 5 times, is ultrasonically treated, ultrasonic time 1min, frequency 100Hz, then with the screen filtration of 800 mesh, obtains fermentation caprophyl
Liquid；Prepare feed for the bamboo rat with symptom of diarrhea, 20g carrots, 18g bamboo branch are chopped into 2cm, it is green with 8g niblets, 7g
Beans, 2g peanuts, 0.1g Radix Angelicae Sinensis, 0.2g palms, 0.1g Jasmines are uniformly mixed；Fermentation caprophyl liquid and the feed of sufferer bamboo rat are pressed
4ml：The proportioning of 200g is sprayed, and is stirred 1min, is fed to the bamboo rat with symptom of diarrhea.
Embodiment 2
Choose the female bamboo rat of adult for not suffering from symptom of diarrhea, healthy growth, continuous 28 days feeding conditioning-type feeds, conditioning-type
Feed is maize straw 42g, hybrid giant napier bar 22g, bamboo root 16g, watermelon peel 9g, sweet potato 7g, peanut 8g, soya bean 4g；After 28 days,
The excrement that bamboo rat drains naturally is collected immediately after in vitro；The excrement of collection and the sodium citrate solution of 0.5wt.% are pressed
Mass ratio 10:1 mixing, stirs 5min, and ferment 4h under 36 DEG C of aseptic condition, then is diluted to fermentation caprophyl with physiological saline
It 5 times, is ultrasonically treated, ultrasonic time 2min, frequency 125Hz, then with the screen filtration of 900 mesh, obtains fermentation caprophyl
Liquid；Prepare feed for the bamboo rat with symptom of diarrhea, 23g carrots, 20g bamboo branch are chopped into 2cm, it is green with 9g niblets, 8g
Beans, 3g peanuts, 0.2g Radix Angelicae Sinensis, 0.2g palms, 0.1g Jasmines are uniformly mixed；Fermentation caprophyl liquid and the feed of sufferer bamboo rat are pressed
5ml：The proportioning of 200g is sprayed, and is stirred 2min, is fed to the bamboo rat with symptom of diarrhea.
Embodiment 3
Choose the female bamboo rat of adult for not suffering from symptom of diarrhea, healthy growth, continuous 30 days feeding conditioning-type feeds, conditioning-type
Feed is maize straw 60g, hybrid giant napier bar 24g, bamboo root 18g, watermelon peel 10g, sweet potato 9g, peanut 9g, soya bean 6g；At 30 days
Afterwards, the excrement that bamboo rat drains naturally is collected immediately after in vitro；The excrement of collection and the sodium citrate solution of 0.7wt.%
In mass ratio 10:1 mixing, stirs 5min, and ferment 5h under 37 DEG C of aseptic condition, then will fermentation caprophyl dilution with physiological saline
It to 5 times, is ultrasonically treated, ultrasonic time 3min, frequency 150Hz, then with the screen filtration of 1000 mesh, obtains fermentation excrement
Bacterium solution；Prepare feed for the bamboo rat with symptom of diarrhea, 25g carrots, 22g bamboo branch is chopped into 3cm, with 10g niblets, 9g
Mung bean, 4g peanuts, 0.3g Radix Angelicae Sinensis, 0.2g palms, 0.1g Jasmines are uniformly mixed；By the feed of caprophyl liquid and sufferer bamboo rat that ferments
By 6ml：The proportioning of 200g is sprayed, and is stirred 3min, is fed to the bamboo rat with symptom of diarrhea.
Embodiment 4
Choose the adult hero bamboo rat for not suffering from symptom of diarrhea, healthy growth, continuous 27 days feeding conditioning-type feeds, conditioning-type
Feed is maize straw 48g, hybrid giant napier bar 21g, bamboo root 16g, watermelon peel 8g, sweet potato 6g, peanut 9g, soya bean 4g；After 27 days,
The excrement that bamboo rat drains naturally is collected immediately after in vitro；The excrement of collection and the sodium citrate solution of 0.4wt.% are pressed
Mass ratio 10:1 mixing, stirs 5min, and ferment 3h under 35 DEG C of aseptic condition, then is diluted to fermentation caprophyl with physiological saline
5 times, be ultrasonically treated, ultrasonic time be 1~3min, frequency 100Hz, then use the screen filtration of 850 mesh, obtain ferment excrement
Bacterium solution；Prepare feed for the bamboo rat with symptom of diarrhea, 24g carrots, 20g bamboo branch are chopped into 2cm, it is green with 9g niblets, 7g
Beans, 2g peanuts, 0.3g Radix Angelicae Sinensis, 0.2g palms, 0.1g Jasmines are uniformly mixed；Fermentation caprophyl liquid and the feed of sufferer bamboo rat are pressed
4ml：The proportioning of 200g is sprayed, and is stirred 1~3min, is fed to the bamboo rat with symptom of diarrhea.
Embodiment 5
Choose the adult hero bamboo rat for not suffering from symptom of diarrhea, healthy growth, continuous 27 days feeding conditioning-type feeds, conditioning-type
Feed is maize straw 49g, hybrid giant napier bar 21g, bamboo root 18g, watermelon peel 9g, sweet potato 5g, peanut 8g, soya bean 3g；After 27 days,
The excrement that bamboo rat drains naturally is collected immediately after in vitro；The excrement of collection and the sodium citrate solution of 0.7wt.% are pressed
Mass ratio 10:1 mixing, stirs 5min, and ferment 4h under 37 DEG C of aseptic condition, then is diluted to fermentation caprophyl with physiological saline
It 5 times, is ultrasonically treated, ultrasonic time 2min, frequency 120Hz, then with the screen filtration of 950 mesh, obtains fermentation caprophyl
Liquid；Prepare feed for the bamboo rat with symptom of diarrhea, 34g carrots, 21g bamboo branch are chopped into 4cm, it is green with 9g niblets, 8g
Beans, 3g peanuts, 0.3g Radix Angelicae Sinensis, 0.2g palms, 0.1g Jasmines are uniformly mixed；Fermentation caprophyl liquid and the feed of sufferer bamboo rat are pressed
6ml：The proportioning of 200g is sprayed, and is stirred 2min, is fed to the bamboo rat with symptom of diarrhea.
Embodiment 6
Choose the childhood female bamboo rat for not suffering from symptom of diarrhea, healthy growth, continuous 28 days feeding conditioning-type feeds, conditioning-type
Feed is maize straw 53g, hybrid giant napier bar 21g, bamboo root 16g, watermelon peel 9g, sweet potato 7g, peanut 6g, soya bean 3g；After 28 days,
The excrement that bamboo rat drains naturally is collected immediately after in vitro；The excrement of collection and the sodium citrate solution of 0.5wt.% are pressed
Mass ratio 10:1 mixing, stirs 5min, and ferment 5h under 35 DEG C of aseptic condition, then is diluted to fermentation caprophyl with physiological saline
It 5 times, is ultrasonically treated, ultrasonic time 2min, frequency 135Hz, then with the screen filtration of 850 mesh, obtains fermentation caprophyl
Liquid；Prepare feed for the bamboo rat with symptom of diarrhea, 23g carrots, 19g bamboo branch are chopped into 2cm, it is green with 9g niblets, 7g
Beans, 4g peanuts, 0.3g Radix Angelicae Sinensis, 0.2g palms, 0.1g Jasmines are uniformly mixed；Fermentation caprophyl liquid and the feed of sufferer bamboo rat are pressed
6ml：The proportioning of 200g is sprayed, and is stirred 3min, is fed to the bamboo rat with symptom of diarrhea.
Embodiment 7
Choose the childhood hero bamboo rat for not suffering from symptom of diarrhea, healthy growth, continuous 28 days feeding conditioning-type feeds, conditioning-type
Feed is maize straw 40g, hybrid giant napier bar 21g, bamboo root 15g, watermelon peel 10g, sweet potato 9g, peanut 6g, soya bean 5g；At 28 days
Afterwards, the excrement that bamboo rat drains naturally is collected immediately after in vitro；The excrement of collection and the sodium citrate solution of 0.6wt.%
In mass ratio 10:1 mixing, stirs 5min, and ferment 5h under 36 DEG C of aseptic condition, then will fermentation caprophyl dilution with physiological saline
It to 5 times, is ultrasonically treated, ultrasonic time is 1~3min, frequency 130Hz, then with the screen filtration of 900 mesh, is fermented
Caprophyl liquid；For with symptom of diarrhea bamboo rat prepare feed, 25g carrots, 19g bamboo branch are chopped into 2cm, with 10g niblets,
7g mung beans, 3g peanuts, 0.1g Radix Angelicae Sinensis, 0.2g palms, 0.1g Jasmines are uniformly mixed；By the feeding of caprophyl liquid and sufferer bamboo rat of fermenting
Material presses 6ml：The proportioning of 200g is sprayed, and is stirred 1min, is fed to the bamboo rat with symptom of diarrhea.
Test example
200 bamboo rats for suffering from symptom of diarrhea are chosen, are randomly divided into 10 groups, every group of half male and half female, the 1st~7 group is adopted successively
Illness bamboo rat is treated with the caprophyl transplantation method of embodiment 1-7, the 8th group uses Norfloxacin with 0.02mg/kg/ days
Dosage treats illness bamboo rat, and the 9th group is treated illness bamboo rat using method disclosed in CN201310632906.0,
10th group does not use any therapeutic modality.
Observe therapeutic effect, count treatment results, judged with invalid, effective, recovery from illness grade III Standard, calculate it is efficient,
Simultaneously record each group illness bamboo rat be averaged effective time, average cured time and fully recover 10 days after body weight evolution.As a result
It is shown in Table 1.
Effective percentage=(effective number+recovery from illness number)/total number of cases × 100%
The therapeutic effect of 1 bamboo rat of table
As can be seen from the above table, the therapy that embodiment 1-3 is used has preferable therapeutic effect, efficient reachable
To 85%~90%, and be averaged responding time and average cured time it is shorter, bamboo rat recovery from illness after body weight growth rate it is higher, it is extensive
Multiple effect is good.
Claims (8)
1. a kind of method for treating bamboo rat diarrhea using caprophyl implantation technique, which is characterized in that use and do not suffer from symptom of diarrhea
Bamboo rat excrement is mixed into feed, and feeding suffers from the bamboo rat of symptom of diarrhea.
2. as described in claim 1 using caprophyl implantation technique treatment bamboo rat diarrhea method, which is characterized in that it is described not
Bamboo rat with symptom of diarrhea is the female bamboo rat that grows up.
3. utilizing the method for caprophyl implantation technique treatment bamboo rat diarrhea as described in claim 1, which is characterized in that the bamboo
Mouse excrement is the natural excreta that bamboo rat continuously feeds conditioning-type feed gained after 25~30 days.
4. utilizing the method for caprophyl implantation technique treatment bamboo rat diarrhea as claimed in claim 3, which is characterized in that the tune
Reason type feed includes following component in parts by mass：
35~60 parts of maize straw, 20~24 parts of hybrid giant napier bar, 15~18 parts of bamboo root, 8~10 parts of watermelon peel, 5~9 parts of sweet potato,
6~9 parts of peanut, 3~6 parts of soya bean.
5. utilizing the method for caprophyl implantation technique treatment bamboo rat diarrhea as described in claim 1, which is characterized in that the bamboo
Mouse excrement is mixed into feed, includes the following steps：
(1) sodium citrate solution of 0.3~0.7wt.% is sprayed to the bamboo rat excrement after in vitro, bamboo rat excrement and sodium citrate are molten
The mass ratio of liquid is 10:1,5min is stirred, ferment 3~5h under 34~37 DEG C of aseptic condition, obtains fermentation excrement；
(2) fermentation caprophyl is diluted to 5 times with physiological saline, is ultrasonically treated, ultrasonic time is 1~3min, frequency 100
~150Hz, then with the screen filtration of 800~1000 mesh, obtain fermentation caprophyl liquid；
(3) fermentation caprophyl liquid sprays feed, stirs 1~3min, you can.
6. utilizing the method for caprophyl implantation technique treatment bamboo rat diarrhea as claimed in claim 5, which is characterized in that fermentation caprophyl
The proportioning of liquid and feed is 4~6ml：200g.
7. utilizing the method for caprophyl implantation technique treatment bamboo rat diarrhea as described in claim 1, which is characterized in that the feeding
Material, including following preparation process：
20~25 portions of carrots, 18~22 parts of bamboo branch are chopped into 2~3cm, with 8~10 parts of niblets, 7~9 parts of mung beans, 2~4
Part peanut is uniformly mixed.
8. utilizing the method for caprophyl implantation technique treatment bamboo rat diarrhea as described in claim 1, which is characterized in that the feeding
Material is also added with 0.1~0.3 part of Radix Angelicae Sinensis, 0.2 portion of palm, 0.1 part of Jasmine.
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| CN105029031A (en) * | 2015-07-24 | 2015-11-11 | 广西大学 | Method for improving growth performance of weaned pigs and reducing diarrhea rate through coprophilous fungus transplantation |
| CN106561541A (en) * | 2015-10-10 | 2017-04-19 | 紫云自治县宏祥竹鼠养殖有限公司 | Cultivation method of bamboo rats |
| CN107182925A (en) * | 2017-06-12 | 2017-09-22 | 浙江大学 | Faecal microbiota implantation technique and its application |
| CN107467352A (en) * | 2017-09-30 | 2017-12-15 | 东兰县三弄瑶山生态竹鼠养殖专业合作社 | A kind of ecological bamboo rat feed |
| CN107593597A (en) * | 2017-09-30 | 2018-01-19 | 东兰县三弄瑶山生态竹鼠养殖专业合作社 | A kind of bamboo rat method for breeding |
| CN107787915A (en) * | 2017-10-26 | 2018-03-13 | 南宁市浩发科技有限公司 | A kind of artificial cultivating method of bamboo rat |
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