CN108587858A - Dispense the priming device of culture medium - Google Patents
Dispense the priming device of culture medium Download PDFInfo
- Publication number
- CN108587858A CN108587858A CN201810282861.1A CN201810282861A CN108587858A CN 108587858 A CN108587858 A CN 108587858A CN 201810282861 A CN201810282861 A CN 201810282861A CN 108587858 A CN108587858 A CN 108587858A
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- China
- Prior art keywords
- casing
- liquid injecting
- injecting mouth
- culture medium
- bearing
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Supply Of Fluid Materials To The Packaging Location (AREA)
Abstract
The present invention relates to culture medium preparing technical fields, disclose a kind of priming device of packing culture medium, including liquid injection pipe, liquid injecting mouth, casing and bearing, described liquid injection pipe one end connects medium container, one end connects the liquid injecting mouth, the liquid injecting mouth is inserted into described sleeve pipe, the related liquid injecting mouth of casing is inserted into the jack opened up on bearing, the bearing is mounted on culture medium packaging device, the internal diameter of described sleeve pipe is more than the outer diameter of liquid injecting mouth, the rear end of described sleeve pipe is provided with L-shaped groove, the long edge groove of L-shaped groove is along casing generatrix direction, the front end that preceding short side slot is located at long edge groove is arranged perpendicular to casing busbar along casing circumferencial direction, fixture block is arranged on cylindrical surface in the liquid injecting mouth rear portion, the length of liquid injecting mouth fixture block front end is more than the length of the preceding short side front of the slot of casing, and less than the length of entire casing.The present invention prevents culture medium from freezing and blocks liquid injection pipe in liquid injecting mouth, and efficiently reduces microbiological contamination.
Description
Technical field
The present invention relates to culture medium preparing technical fields, and in particular to be it is a kind of packing culture medium priming device.
Background technology
With the development of Spawn incubation technology, agar medium is being dispensed into the operation in culture dish, just from manual side
Formula gradually develops to automation packing instrument mode to complete, and automation culture medium packing instrument packing agar medium be unable to do without one
Woven hose, by this root woven hose by medium container(Liquid storage bottle or culture medium preparation device)In in melting state culture medium it is defeated
It is sent in the culture dish on packing instrument, after culture medium solidification, solid plate culture medium is made.
Because infusion process needs to provide power with peristaltic pump, so woven hose often uses silica gel hose, because silicone tube material is soft,
It needs to connect with silicone tube by the liquid injecting mouth of a metal material, liquid injecting mouth one end when being inserted and fixed on packing instrument, separately
One end is inserted into the jack on liquid injecting mouth holder.
The woven hose of mating automation culture medium packing instrument currently on the market, is the silica gel of about one meter or so of a root long degree
Pipe connects the stainless steel liquid injecting mouth of a segment length about 15cm.Culture medium first flows through silicone tube in pipe, passes through stainless steel fluid injection
Mouth is finally injected into culture dish.
There are two apparent defects for the liquid injecting mouth of this material and structure type:First, due to the liquid injecting mouth of metal material
Radiating rate is fast, and after culture medium flows through liquid injecting mouth when not actuated packaging operation, culture medium residence time in liquid injecting mouth is slightly long
Culture medium frozen plug in liquid injecting mouth is just easily caused, when especially room temperature is relatively low in winter, this culture medium is being infused
There is a phenomenon where solidifying to happen occasionally in pipe, very big inconvenience in operation is caused, packing failure is even resulted in.Second is that liquid injecting mouth with
It is more closely to coordinate to be fixed on liquid injecting mouth holder between the jack of liquid injecting mouth, when liquid injecting mouth passes through jack on holder, note
Liquid mouth is easy to encounter jack inner wall or other positions on holder, since the disinfection of holder is only wiped with alcohol swab, it is difficult to ensure that
Its integral asepsis, so this link be easy to cause the risk of microbiological contamination.
Invention content
The purpose of the present invention is to solve the above problems, provide a kind of priming device of packing culture medium, prevent from cultivating
Base freezes and blocks liquid injection pipe in liquid injecting mouth, and efficiently reduces microbiological contamination.
The technical solution adopted by the present invention is that:
A kind of priming device of packing culture medium, characterized in that including liquid injection pipe, liquid injecting mouth, casing and bearing, the liquid injection pipe
One end connects medium container, and one end connects the liquid injecting mouth, and the liquid injecting mouth is inserted into described sleeve pipe, the related liquid injecting mouth of casing
It is inserted into the jack opened up on bearing, the bearing is mounted on culture medium packaging device, and the internal diameter of described sleeve pipe is more than fluid injection
The rear end of the outer diameter of mouth, described sleeve pipe is provided with L-shaped groove, and for the long edge groove of L-shaped groove along casing generatrix direction, preceding short side slot is located at long side
The front end of slot is arranged perpendicular to casing busbar along casing circumferencial direction, and fixture block is arranged on cylindrical surface in the liquid injecting mouth rear portion, described
The length of liquid injecting mouth fixture block front end is more than the length of the preceding short side front of the slot of casing, and less than the length of entire casing, the note
When liquid mouth is inserted into described sleeve pipe, the fixture block coordinates after the long edge groove of L-shaped groove in preceding short side slot.
Further, close to the back-end location of long edge groove in described sleeve pipe, short side slot after setting, it is described after short side slot it is vertical
Short side slot and preceding short side slot are in the same direction or incorgruous after casing busbar is arranged along casing circumferencial direction, is described.
Further, the fixture block and long edge groove clearance fit, with preceding short side slot and rear short side slot snap-fit.
Further, the front end of the liquid injecting mouth is taper.
Further, limiting slot is arranged in the middle part of described sleeve pipe, position-limitting pin is arranged on bearing, when casing is together with liquid injecting mouth
During insertion bearing jack is pushed ahead, when the limiting slot on casing reaches exactly to position-limitting pin position, under the effect of elasticity, limit
Pin is withstood in limiting slot.
Further, the liquid injecting mouth is rigid plastics material, and described sleeve pipe is metal or rigid plastics material, the fluid injection
The material of pipe is one kind in polypropylene, makrolon, polystyrene, polyethylene.
Further, the bearing is metal material, and there are one trepannings for bearing lower section, and a heating rod is inserted into trepanning, is added
Hot pin can be according to setting or the temperature of feedback control bearing, and by temperature conduction to casing.
Further, the liquid injection pipe includes internal layer, middle layer and outer layer, and the middle layer is carbon fiber layer, the carbon fiber
Dimension layer is connected to electronic control unit, and the electronic control unit is heated by carbon fiber layer to liquid injection pipe.
The beneficial effects of the invention are as follows:
(1)It before liquid injecting mouth is inserted into bearing, contracts in casing, after casing is inserted into bearing, then liquid injecting mouth is stretched out into casing, prevent fluid injection
Microbiological contamination in mouth insertion process;
(2)Liquid injection pipe uses carbon fiber heating, bearing also to have heating function, and retaining sleeve and liquid injecting mouth temperature are constant, make fluid injection
Device(A whole set of infusion pipeline)Interior culture solution will not generate solidification because of low ambient temperature.
Description of the drawings
Attached drawing 1 is the general assembly structural schematic diagram of the present invention;
Attached drawing 2 is another visual angle schematic diagram of total assembling structure of the present invention;
Attached drawing 3 is the structural schematic diagram of liquid injecting mouth;
Attached drawing 4 is the structural schematic diagram of casing;
Attached drawing 5 is the structural schematic diagram of bearing;
Attached drawing 6 is the diagrammatic cross-section of liquid injection pipe;
Attached drawing 7 is the structural schematic diagram that liquid injection pipe is retracted to casing;
Attached drawing 8 is the structural schematic diagram that liquid injection pipe stretches out casing.
Label in attached drawing is respectively:
1. liquid injection pipe;2. liquid injecting mouth;
3. bearing;4. jack;
5. casing;6. fixture block;
7. flange;8. long edge groove;
Short side slot before 9.;Short side slot after 10.;
11. limiting slot;12. position-limitting pin;
13. heating rod;14. internal layer;
15. middle layer.16. outer layer.
Specific implementation mode
It elaborates below in conjunction with the accompanying drawings to the specific implementation mode of the priming device of present invention packing culture medium.
Referring to attached drawing 1,2, the priming device for dispensing culture medium includes liquid injection pipe 1, liquid injecting mouth 2, casing 5 and bearing 3, fluid injection
1 one end of pipe connects medium container, and one end connects liquid injecting mouth 2, and bearing 3 is mounted on culture medium packaging device, is provided on bearing 3
Jack 4, casing 5 can be inserted on bearing in jack 4 when use, and be limited slot 11 and position-limitting pin 12 coordinates, and limit casing 5
It is inserted into the accurate location of bearing 3, L-shaped groove is provided on casing 5, two screens, the first screens are realized in 2 plug-in-sleeve 5 of liquid injecting mouth
It is not fitted into L-shaped groove for the fixture block 6 on liquid injecting mouth 2, the second screens is that fixture block 6 is engaged in L-shaped groove, realizes liquid injecting mouth 2
Extend and retract two stations.
Referring to attached drawing 3, liquid injecting mouth 2 is tubular structure, and rigid plastics material, front end is conical head, facilitate plug-in-sleeve and
Culture dish, the rear end ladder diameter of axle, rear end forms flange 7, convenient to be more firmly socketed with liquid injection pipe 1.At the middle part of liquid injecting mouth 2
Fixture block 6 is set close to back-end location, fixture block 6 is box-shaped or cylinder head.
Referring to attached drawing 4, casing 5 is tubulose, using metal or rigid plastics material, preferably 316 or more stainless steel.
Internal diameter is slightly larger than the maximum outside diameter of liquid injecting mouth 2, is in gap fiting mode when liquid injecting mouth 2 is inserted into, and the rear end of casing 5 is provided with L-shaped groove,
For the long edge groove 8 of L-shaped groove along 5 generatrix direction of casing, preceding short side slot 9 is located at the front end of long edge groove 8 perpendicular to 5 busbar of casing along casing
5 circumferencial directions are arranged.When the preceding short side slot 9 of L-shaped groove is located at the second screens for liquid injecting mouth 2, the liquid injecting mouth 2 in casing 5 is stretched into
It is locked after rotation.In order to which in the first screens, also there are one good stationary states for liquid injecting mouth 2, close to long edge groove 8 on casing 5
Back-end location, short side slot 10 after setting, rear short side slot 10 perpendicular to 5 busbar of casing along 5 circumferencial direction of casing be arranged, rear short side
Slot 10 and preceding short side slot 9 are in the same direction or incorgruous.When liquid injecting mouth 2 is located at the first screens, same rotation to fixture block 6 and rear short side slot 10 is matched
It realizes and fixes when conjunction.Fixture block 6 and 8 clearance fit of long edge groove are realized quick with preceding short side slot 9 and rear 10 snap-fit of short side slot
Switching, secure lock.Limiting slot 11 is arranged in the middle part of casing 5, and the position of bearing 3 is inserted into for limiting casing 5.
The length of casing 5 and liquid injecting mouth 2 is set as following relationship:The length of 2 fixture block of liquid injecting mouth, 6 front end is more than casing 5
The length of preceding 9 front end of short side slot, and less than the length of entire casing 5.
Referring to attached drawing 7,8, when 2 plug-in-sleeve 5 of liquid injecting mouth, be presented two kinds of location status, i.e. when the first screens position, fluid injection
The head of mouth 2 is retracted in casing 5, and when the second screens position, the head of liquid injecting mouth 2 is stretched out outside casing 5.
Referring to attached drawing 5,3 bottom of bearing is fixed by fastener and culture medium packaging device, and jack 4 is arranged in top, is used for
Position-limitting pin 12 is arranged in 4 side of jack in plug-in-sleeve 5, and after casing 5 is inserted into, position-limitting pin 12 coordinates the limiting slot in casing 5
In 11, casing 5 is fixed in the jack 4 of bearing 3, while playing the role of limit.Bearing 3 is metal material, bearing lower section
There are one trepannings, and a heating rod 13 is inserted into trepanning, and heating rod 13 is connected to power supply, and heating rod 13 can be according to setting or feedback
The temperature of bearing is controlled, and by temperature conduction to casing, maintains liquid injecting mouth temperature constant.
A kind of material in polypropylene, makrolon, polystyrene, polyethylene is selected referring to attached drawing 6, liquid injection pipe 1, with note
The rear end resilient engagement of liquid mouth 2 ensures leakproofness.Liquid injection pipe 1 includes internal layer 14, middle layer 15 and outer layer 16, and middle layer 15 is
Carbon fiber layer, internal layer are silica gel material, and outer layer is thermal insulation material.Carbon fiber layer is connected to electronic control unit, and electronic control unit passes through carbon
Fibrous layer is heated to liquid injection pipe 1, and heating temperature is no more than 55 DEG C.
Referring to attached drawing 7,8, the process for carrying out filling culture solution to culture dish by liquid injecting mouth 2 is as follows:One end of liquid injection pipe 1
It is connected to peristaltic pump, peristaltic pump extracts culture solution in medium container, and the other end is connect with liquid injecting mouth 2.Card on liquid injecting mouth 2
Block 6 is engaged to the rear end of L-shaped groove, to 10 position of front to back short side slot, rotates liquid injecting mouth 2, fixture block 6 is made to block with rear short side slot 10
Tightly, at this moment, the front end of liquid injecting mouth 2 is located in casing 5, wrapping sterilizing.Before fluid injection, liquid injecting mouth 2 is inserted into branch under the protection of casing 5
Seat 3, casing 5 is fixed on by the position-limitting pin 12 on bearing 3 in the jack 4 of bearing 3.When packaging device works, by fluid injection
Mouth 2 is rotated to negative direction, so that fixture block 6 is left rear short side slot 10, is then forward extended out, fixture block 6 is made to be located at the front top of L-shaped groove
Preceding 9 position of short side slot, rotate liquid injecting mouth 2, short side slot 9 before making fixture block 6 be caught in front, at this moment liquid injecting mouth 2 head stretch out casing
5, and extend into packing instrument right over culture dish ware bottom, it waits to be injected.After the completion of fluid injection work, reverse operating makes fluid injection
It retracts the front end of mouth 2.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
Member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (9)
1. a kind of priming device of packing culture medium, it is characterised in that:Including liquid injection pipe, liquid injecting mouth, casing and bearing, the note
Liquid pipe one end connects medium container, and one end connects the liquid injecting mouth, and the liquid injecting mouth is inserted into described sleeve pipe, the related note of casing
Liquid mouth is inserted into the jack opened up on bearing, and the bearing is mounted on culture medium packaging device, and the internal diameter of described sleeve pipe is more than
The rear end of the outer diameter of liquid injecting mouth, described sleeve pipe is provided with L-shaped groove, and along casing generatrix direction, preceding short side slot is located at the long edge groove of L-shaped groove
The front end of long edge groove is arranged perpendicular to casing busbar along casing circumferencial direction, and fixture block is arranged on cylindrical surface in the liquid injecting mouth rear portion,
The length of liquid injecting mouth fixture block front end is more than the length of the preceding short side front of the slot of casing, and less than the length of entire casing, institute
When stating liquid injecting mouth insertion described sleeve pipe, the fixture block coordinates after the long edge groove of L-shaped groove in preceding short side slot.
2. the priming device of packing culture medium according to claim 1, it is characterised in that:Close to long side in described sleeve pipe
The back-end location of slot, short side slot after setting, the rear short side slot is arranged perpendicular to casing busbar along casing circumferencial direction, after described
Short side slot and preceding short side slot are in the same direction or incorgruous.
3. the priming device of packing culture medium according to claim 2, it is characterised in that:The fixture block and long edge groove gap
Cooperation, with preceding short side slot and rear short side slot snap-fit.
4. the priming device of packing culture medium according to claim 1, it is characterised in that:The front end of the liquid injecting mouth is cone
Shape.
5. the priming device of packing culture medium according to claim 1, it is characterised in that:The middle part setting limit of described sleeve pipe
Position slot, is arranged position-limitting pin on bearing, in casing is pushed ahead together with liquid injecting mouth insertion bearing jack, the limit on casing
When slot reaches exactly to position-limitting pin position, under the effect of elasticity, position-limitting pin is withstood in limiting slot.
6. the priming device of packing culture medium according to any one of claim 1 to 5, it is characterised in that:The fluid injection
Mouth is rigid plastics material, and described sleeve pipe is metal or rigid plastics material, and the material of the liquid injection pipe is polypropylene, poly- carbonic acid
One kind in ester, polystyrene, polyethylene.
7. the priming device of packing culture medium according to claim 6, it is characterised in that:The bearing is metal material,
There are one trepannings below bearing, are inserted into a heating rod in trepanning, heating rod can according to setting or the temperature of feedback control bearing,
And by temperature conduction to casing.
8. the priming device of packing culture medium according to any one of claim 1 to 5, it is characterised in that:The fluid injection
Pipe includes internal layer, middle layer and outer layer, and the middle layer is carbon fiber layer, and the carbon fiber layer is connected to electronic control unit, described
Electronic control unit is heated by carbon fiber layer to liquid injection pipe.
9. the priming device of packing culture medium according to claim 8, it is characterised in that:The internal layer is silica gel material,
Outer layer is thermal insulation material.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201810282861.1A CN108587858B (en) | 2018-04-02 | 2018-04-02 | Priming device of partial shipment culture medium |
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CN201810282861.1A CN108587858B (en) | 2018-04-02 | 2018-04-02 | Priming device of partial shipment culture medium |
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CN108587858A true CN108587858A (en) | 2018-09-28 |
CN108587858B CN108587858B (en) | 2023-09-19 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110841738A (en) * | 2019-11-26 | 2020-02-28 | 张丽珠 | Liquid injector for subpackaging culture media |
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CN110841738A (en) * | 2019-11-26 | 2020-02-28 | 张丽珠 | Liquid injector for subpackaging culture media |
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