CN108575915B - Cold storage anesthesia transportation release method for mosquitoes - Google Patents
Cold storage anesthesia transportation release method for mosquitoes Download PDFInfo
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- CN108575915B CN108575915B CN201810345992.XA CN201810345992A CN108575915B CN 108575915 B CN108575915 B CN 108575915B CN 201810345992 A CN201810345992 A CN 201810345992A CN 108575915 B CN108575915 B CN 108575915B
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- 241000255925 Diptera Species 0.000 title claims abstract description 179
- 206010002091 Anaesthesia Diseases 0.000 title claims abstract description 53
- 230000037005 anaesthesia Effects 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000032669 eclosion Effects 0.000 claims abstract description 125
- 238000005057 refrigeration Methods 0.000 claims abstract description 63
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 238000004806 packaging method and process Methods 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 8
- 241000238631 Hexapoda Species 0.000 claims 5
- 238000011084 recovery Methods 0.000 description 36
- 208000001490 Dengue Diseases 0.000 description 3
- 206010012310 Dengue fever Diseases 0.000 description 3
- 241000604961 Wolbachia Species 0.000 description 3
- 208000025729 dengue disease Diseases 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 241000256173 Aedes albopictus Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002618 waking effect Effects 0.000 description 2
- 241000725619 Dengue virus Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003444 anaesthetic effect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Animal Behavior & Ethology (AREA)
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- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses a method for releasing mosquitoes in refrigeration anesthesia transportation, which comprises the following steps: A. loading male pupae into an eclosion barrel or an eclosion cage, adding water into the eclosion barrel or the eclosion cage, and naturally eclosion of the male pupae into male mosquitoes in the eclosion barrel or the eclosion cage; B. draining water in the eclosion barrel or the eclosion cage, naturally drying the eclosion barrel or the eclosion cage and male mosquitoes, and simultaneously adjusting the density of the male mosquitoes in the eclosion barrel or the eclosion cage; C. placing the eclosion barrel or the eclosion cage into a first refrigeration house for refrigeration, and enabling male mosquitoes to enter an anesthesia state; D. transferring the anesthetized male mosquitoes into a second refrigeration house, and placing the anesthetized male mosquitoes in a release box in the second refrigeration house for packaging; E. transporting the release cartridge to a release location for release; F. the released male mosquitoes quickly recover flight ability at room temperature. The volume of the package of the anesthetized male mosquitoes is reduced, the packaging and transportation cost is reduced, and the anesthetized male mosquitoes are convenient to release.
Description
Technical Field
The invention relates to the field of mosquito control, in particular to a method for releasing mosquitoes through refrigeration, anesthesia and transportation.
Background
In the existing prevention and control of dengue fever, because of the lack of specific drugs and effective vaccines for resisting dengue virus, the main method for preventing and controlling dengue fever is to control the quantity of vector aedes albopictus. In inland China, a biological control method is developed aiming at the situation, namely, the method for controlling mosquitoes by using mosquitoes, the method establishes a novel Wolbachia infection mosquito strain through embryo microinjection, the strain can be spread to offspring through a mother generation, and when male mosquitoes carrying the Wolbachia are mated with wild female mosquitoes, mosquito eggs laid by the wild female mosquitoes can not develop. Therefore, the population quantity of Aedes albopictus can be effectively controlled by continuously releasing the male mosquitoes carrying Wolbachia albopictus, thereby achieving the aim of preventing dengue fever.
Before male mosquitoes are released, the male pupae which are not feathered are generally placed in a release cage or a release cage firstly, so that the male pupae are naturally feathered into the male mosquitoes, then the release cage or the release cage is transported to a release place to be released, in the process, in order to ensure the survival rate of the male mosquitoes in the release cage or the release cage, the density of the male mosquitoes in the release cage or the release cage is generally smaller, so that more release cages or release cages can be filled with fewer male mosquitoes, the cost of the release cage or the release cage is increased, the transportation cost of the release cage or the release cage is increased, and the male mosquitoes are in a waking state when being released, so that the release difficulty is increased.
Disclosure of Invention
The invention aims to provide a method for transporting and releasing mosquitoes in a cold storage and anesthesia way, which reduces the required volume when packing the same number of male mosquitoes, saves the cost of a releasing box and the transportation cost, and simultaneously facilitates the releasing of the anesthetized male mosquitoes.
The technical scheme is as follows:
the invention discloses a method for releasing mosquitoes in refrigeration anesthesia transportation, which comprises the following steps:
A. loading male pupae into an emergence barrel or an emergence cage, adding water into the emergence barrel or the emergence cage, and allowing the male pupae to naturally emerge into male mosquitoes in the emergence barrel or the emergence cage;
B. draining water in the eclosion barrel or the eclosion cage, naturally drying the eclosion barrel or the eclosion cage and the male mosquitoes, and simultaneously adjusting the density of the male mosquitoes in the eclosion barrel or the eclosion cage;
C. placing the eclosion barrel or the eclosion cage into a first refrigeration house for refrigeration, and enabling the male mosquitoes to enter an anesthesia state;
D. transferring the anesthetized male mosquitoes to a second refrigeration house, and placing the anesthetized male mosquitoes in a release box in the second refrigeration house for packaging;
E. transporting the release cartridge to a release location for release;
F. the released male mosquitoes rapidly recover flight ability at room temperature.
In the step C, the temperature of the first refrigeration house is 1-10 ℃.
In step C, the average time required for the male mosquito to enter the anesthetic state from the awake state is 2 to 14 minutes.
In step D, the temperature in the second freezer is 1 to 10 degrees.
In the step D, the temperature of the second refrigeration house is 5-10 ℃.
In step E, the temperature of the release cassette during transport is 1 to 10 degrees.
In step E, the temperature of the release cassette during transport is 5 to 10 degrees.
In step a, the density of the male pupae in the emergence barrel or cage is 15 to 25 per cubic centimeter.
In step B, the density of the male mosquitoes in the emergence barrel or cage is 0.16 to 0.2 per cubic centimeter.
The following illustrates the advantages or principles of the invention:
1. the water is added into the eclosion barrel or the eclosion cage, the water ensures the basic survival and the eclosion condition of male pupae, the male pupae can be eclosion into male mosquitoes, the dried eclosion barrel or the eclosion cage is placed into the first refrigeration house, the male mosquitoes are enabled to enter the anesthesia state at low temperature, in order to ensure the recovery rate of the anesthetized male mosquitoes, the male mosquitoes are transferred into the second refrigeration house for collection and packaging, the volume of the release boxes required by the male mosquitoes with the same quantity can be reduced by 500 times relative to the awakening state after anesthesia, the cost of the release boxes is saved, meanwhile, the cost of transporting the release boxes is reduced, the male mosquitoes in the anesthesia state are convenient to release, and the release efficiency is improved.
2. The temperature in the first freezer is 1 degree to 10 degrees, and it guarantees that male mosquito can get into the anesthesia state under this condition, can guarantee cold-stored male mosquito's after the anesthesia recovery rate simultaneously again, and the temperature is less than 1 degree, and the male mosquito after the anesthesia resumes flight ability time longer, and male mosquito may all die at cold-stored anesthesia in-process, and the temperature is higher than 10 degrees, and male mosquito needs can get into the anesthesia state for a long time, or can not get into the anesthesia state completely, reduces anesthesia efficiency.
3. The average time required for the male mosquitoes to enter the anesthesia state from the awakening state is 2 to 14 minutes, the time is short, and the anesthesia efficiency is high.
4. The temperature in the second freezer is 5 degrees to 10 degrees, and the male mosquito after anaesthetizing is packed and stored under the environment of 5 degrees to 10 degrees, and its recovery rate influence to male mosquito is less.
5. The male mosquito can place the transportation in the freezer car when the transportation to the temperature that sets up the freezer car is 5 degrees to 10 degrees, has both guaranteed that the male mosquito can not revive in the transportation, can not influence the recovery rate and the recovery speed of male mosquito at room temperature yet.
6. The density of the male pupae is 15 to 25 per cubic centimeter, the density setting does not influence the eclosion rate of the male pupae, and simultaneously, the space of the eclosion barrel and the eclosion cage can be utilized to the maximum extent.
7. The density of the male mosquitoes in the eclosion barrel and the eclosion cage is 0.16 to 0.2 per cubic centimeter, and the density arrangement ensures the activity space of the male mosquitoes without increasing the death rate of the male mosquitoes.
Detailed Description
The following provides a detailed description of embodiments of the invention.
The first embodiment:
the embodiment discloses a method for releasing cold storage anesthesia transportation of mosquitoes, which comprises the following steps:
A. the male pupae are filled into an eclosion barrel or an eclosion cage, in order to not influence the eclosion rate, the density of the male pupae is kept between 15/cubic centimeter and 25/cubic centimeter, and the optimal density is 20/cubic centimeter, a small amount of water is added into the eclosion barrel or the eclosion cage, in the embodiment, the water in the eclosion barrel or the eclosion cage is about one tenth of that of the eclosion barrel or the eclosion cage, and the male pupae are naturally and completely eclosion into male mosquitoes in the eclosion barrel or the eclosion cage;
B. draining all water in the emergence barrel or the emergence cage, naturally drying the emergence barrel or the emergence cage and male mosquitoes, and simultaneously adjusting the density of the male mosquitoes in the emergence barrel or the emergence cage to ensure that the density of the male mosquitoes is 0.16 to 0.2 per cubic centimeter;
C. placing the eclosion barrel or the eclosion cage into a cold storage, and refrigerating at 5 ℃ for 5-10 minutes to allow male mosquitoes to enter an anesthesia state;
D. placing the anesthetized male mosquitoes in a release box for packaging and storing, wherein the density of the male mosquitoes in the release box can reach 80 to 100 per cubic centimeter due to the small volume of the release box required by the anesthetized male mosquitoes;
E. the packed release box is transported to a release place to be released before release, the male mosquitoes can be transported by adopting a refrigeration house vehicle or placed in the refrigeration house, and the temperature of the refrigeration house vehicle or the refrigeration house is set to be 5 ℃.
F. The flying ability of the male mosquitoes rapidly recovered at room temperature after being released is described in table 1 below, and the male mosquitoes were refrigerated at 5 degrees for 3 hours, the recovery rate of which was 95.3% on average, and the recovery rate of which was 89.5% on average even when the male mosquitoes were refrigerated for more than 24 hours, and the shorter the refrigerating time of the male mosquitoes at the same temperature, the shorter the time taken for the male mosquitoes to recover the flying ability from the anesthetized state at room temperature, the higher the recovery rate.
The advantages of this embodiment:
1. the water is added into the eclosion barrel or the eclosion cage, the water ensures the basic survival and the eclosion condition of male pupae, the male pupae can be eclosion into male mosquitoes, the dry eclosion barrel and the eclosion cage are placed into the first refrigeration house, the male mosquitoes are enabled to enter the anesthesia state at low temperature, in order to ensure that the male mosquitoes after anesthesia can not awaken, the male mosquitoes are transferred into the second refrigeration house for collection and packaging, the male mosquitoes can reduce the volume of the release boxes required by the same number of the male mosquitoes by 500 times relative to the awakening state after anesthesia, the cost of the release boxes is saved, meanwhile, the cost of transporting the release boxes is reduced, the male mosquitoes under the anesthesia state are convenient to release, and the release efficiency is improved.
2. The temperature in the freezer is 5 degrees, and the male mosquito gets into the required time of anesthesia state shorter under 5 degrees conditions, and anesthesia efficiency is high, and the male mosquito is cold-stored packing and transportation under 5 degrees conditions, has both guaranteed that the male mosquito can not revive in packing and transportation, also can not influence the male mosquito rate of recovery and the speed of recovery at room temperature.
3. The density of the male pupae is 15 to 25 per cubic centimeter, the density setting does not influence the eclosion rate of the male pupae, and simultaneously, the space of the eclosion barrel and the eclosion cage can be utilized to the maximum extent.
4. The density of the male mosquitoes in the eclosion barrel and the eclosion cage is 0.16 to 0.2 per cubic centimeter, and the density arrangement ensures the activity space of the male mosquitoes without influencing the death rate of the male mosquitoes.
Second embodiment:
the embodiment discloses a method for releasing cold storage anesthesia transportation of mosquitoes, which comprises the following steps:
A. the male pupae are filled into an eclosion barrel or an eclosion cage, in order to not influence the eclosion rate, the density of the male pupae is kept between 15/cubic centimeter and 25/cubic centimeter, and the optimal density is 20/cubic centimeter, a small amount of water is added into the eclosion barrel or the eclosion cage, in the embodiment, the water of the eclosion barrel or the eclosion cage is about one tenth of that of the eclosion barrel or the eclosion cage, and the male pupae are naturally and completely eclosion into male mosquitoes in the eclosion barrel or the eclosion cage;
B. draining all water in the emergence barrel or the emergence cage, naturally drying the emergence barrel or the emergence cage and male mosquitoes, and simultaneously adjusting the density of the male mosquitoes in the emergence barrel or the emergence cage to ensure that the density of the male mosquitoes is 0.16 to 0.2 per cubic centimeter;
C. placing the eclosion barrel or the eclosion cage into a first refrigeration house for refrigeration, setting the temperature in the first refrigeration house to be 1 ℃, refrigerating for 2.5 to 5.5 minutes, and enabling male mosquitoes to enter an anesthesia state;
D. the anesthetized male mosquitoes are transferred into a second refrigeration house, the temperature in the second refrigeration house is set to be 5 ℃, the male mosquitoes in the anesthetized state are placed in a release box to be packaged and stored in the second refrigeration house, and the density of the male mosquitoes in the release box can reach 80 to 100 per cubic centimeter due to the fact that the volume of the release box required by the anesthetized male mosquitoes is small.
E. Before release, the packed release boxes are transported to a release place to be released, the male mosquitoes can be transported by adopting a refrigeration house vehicle or placed in the refrigeration house, and the temperature of the refrigeration house vehicle or the refrigeration house is set to be 5 ℃;
F. the released male mosquitoes rapidly recovered flight ability at room temperature, as described in table 1 below, the male mosquitoes had an average recovery rate of 95.3% when refrigerated at 5 degrees for 3 hours and an average recovery rate of 89.5% when refrigerated for 24 hours, and the shorter the refrigeration time at the same temperature, the shorter the time taken for the male mosquitoes to recover flight ability from an anesthetized state at room temperature, the higher the recovery rate.
The advantages of this embodiment over the first embodiment are: the temperature of first freezer is low in this embodiment, and the male mosquito is shorter from the time that the state of waking up entering anesthesia state needs, has practiced thrift cold-stored anesthesia time, has improved cold-stored anesthesia efficiency.
The third embodiment:
the embodiment discloses a method for releasing cold storage anesthesia transportation of mosquitoes, which comprises the following steps:
A. the male pupae are filled into an eclosion barrel or an eclosion cage, in order to not influence the eclosion rate, the density of the male pupae is kept between 15/cubic centimeter and 25/cubic centimeter, and the optimal density is 20/cubic centimeter, a small amount of water is added into the eclosion barrel or the eclosion cage, in the embodiment, the water in the eclosion barrel or the eclosion cage is about one tenth of that of the eclosion barrel or the eclosion cage, and the male pupae are naturally and completely eclosion into male mosquitoes in the eclosion barrel or the eclosion cage;
B. draining all water in the emergence barrel or the emergence cage, naturally drying the emergence barrel or the emergence cage and male mosquitoes, and simultaneously adjusting the density of the male mosquitoes in the emergence barrel or the emergence cage to ensure that the density of the male mosquitoes is 0.16 to 0.2 per cubic centimeter;
C. placing the eclosion barrel or the eclosion cage into a cold storage, and refrigerating at 10 ℃ for 4-14 minutes to allow male mosquitoes to enter an anesthesia state;
D. the male mosquitoes in an anesthesia state are placed in a release box for packaging and storage, and the volume of the release box required by the anesthetized male mosquitoes is smaller, so that the density of the male mosquitoes in the release box can reach 80 to 100 mosquitoes/cubic centimeter;
E. before release, the packed release box is transported to a release place to be released, the male mosquitoes can be transported by adopting a refrigeration house vehicle or placed in the refrigeration house, and the temperature of the refrigeration house vehicle or the refrigeration house is set to be 10 ℃;
F. the released male mosquitoes rapidly recover the flight ability at room temperature, as shown in table 1, the male mosquitoes are refrigerated for 3 hours under the condition of 10 degrees, the average recovery rate of the male mosquitoes is 93.8%, the average recovery time is 1.3 minutes to 1.5 minutes, the male mosquitoes are refrigerated for 24 hours under the condition of 10 degrees, the average recovery rate of the male mosquitoes can still reach 88.7%, the average recovery time is 1.3 minutes to 1.5 minutes, the shorter the refrigeration time of the male mosquitoes at the same temperature is, and the shorter the time taken for recovering the flight ability from the anesthesia state at room temperature is, the higher the recovery rate is.
Advantages of this embodiment over the first and second embodiments: the flight recovery time of the anesthetized male mosquitoes from the anesthetized state at room temperature is short, and the recovery speed is high.
The fourth embodiment:
the embodiment discloses a method for releasing cold storage anesthesia transportation of mosquitoes, which comprises the following steps:
A. the male pupae are filled into an eclosion barrel or an eclosion cage, in order to not influence the eclosion rate, the density of the male pupae is kept between 15/cubic centimeter and 25/cubic centimeter, and the optimal density is 20/cubic centimeter, a small amount of water is added into the eclosion barrel or the eclosion cage, in the embodiment, the water of the eclosion barrel or the eclosion cage is about one tenth of that of the eclosion barrel or the eclosion cage, and the male pupae are naturally and completely eclosion into male mosquitoes in the eclosion barrel or the eclosion cage;
B. draining all water in the emergence barrel or the emergence cage, naturally drying the emergence barrel or the emergence cage and male mosquitoes, and simultaneously adjusting the density of the male mosquitoes in the emergence barrel or the emergence cage to ensure that the density of the male mosquitoes is 0.16 to 0.2 per cubic centimeter;
C. placing the eclosion barrel or the eclosion cage into a first refrigeration house for refrigeration, setting the temperature in the first refrigeration house to be 5 ℃, refrigerating for 5 to 10 minutes, and enabling male mosquitoes to enter an anesthesia state;
D. the anesthetized male mosquitoes are transferred into a second refrigeration house, the temperature in the second refrigeration house is set to be 10 ℃, the male mosquitoes in the anesthetized state are placed in a release box to be packaged and stored in the second refrigeration house, and the density of the male mosquitoes in the release box can reach 80 to 100 per cubic centimeter due to the fact that the volume of the release box required by the anesthetized male mosquitoes is small.
E. Before release, the packed release box is transported to a release place to be released, the male mosquitoes can be transported by adopting a refrigeration house vehicle or placed in the refrigeration house, and the temperature of the refrigeration house vehicle or the refrigeration house is set to be 10 ℃;
F. as shown in table 1, the average recovery rate of the male mosquitoes after the male mosquitoes are refrigerated for 3 hours at 10 degrees is 93.8%, the average recovery time is 1.3 to 1.5 minutes, the average recovery rate of the male mosquitoes after the male mosquitoes are refrigerated for 24 hours at 10 degrees is 88.7%, the average recovery time is 1.3 to 1.5 minutes, and the shorter the refrigeration time of the male mosquitoes at the same temperature is, the shorter the time it takes to recover the flight ability from the anesthesia state at room temperature is, the higher the recovery rate is.
The advantages of this embodiment over the first to third embodiments are: compared with the first embodiment and the second embodiment, in this embodiment, the time taken by the male mosquitoes to recover the flight ability from the anesthesia state at room temperature is short, the recovery speed is fast, and compared with the third embodiment, the time taken by the male mosquitoes to enter the anesthesia state from the awakening state is short, and the anesthesia efficiency is high.
Fifth embodiment:
the embodiment discloses a method for releasing cold storage anesthesia transportation of mosquitoes, which comprises the following steps:
A. the male pupae are filled into an eclosion barrel or an eclosion cage, in order to not influence the eclosion rate, the density of the male pupae is kept between 15/cubic centimeter and 25/cubic centimeter, and the optimal density is 20/cubic centimeter, a small amount of water is added into the eclosion barrel or the eclosion cage, in the embodiment, the water of the eclosion barrel or the eclosion cage is about one tenth of that of the eclosion barrel or the eclosion cage, and the male pupae are naturally and completely eclosion into male mosquitoes in the eclosion barrel or the eclosion cage;
B. draining all water in the emergence barrel or the emergence cage, naturally drying the emergence barrel or the emergence cage and male mosquitoes, and simultaneously adjusting the density of the male mosquitoes in the emergence barrel or the emergence cage to ensure that the density of the male mosquitoes is 0.16 to 0.2 per cubic centimeter;
C. placing the eclosion barrel or the eclosion cage into a first refrigeration house for refrigeration, setting the temperature in the first refrigeration house to be 1 ℃, refrigerating for 2.5 to 5.5 minutes, and enabling male mosquitoes to enter an anesthesia state;
D. the anesthetized male mosquitoes are transferred into a second refrigeration house, the temperature in the second refrigeration house is set to be 10 ℃, the male mosquitoes in the anesthetized state are placed in a release box to be packaged, and the density of the male mosquitoes in the release box can reach 80 to 100 per cubic centimeter due to the fact that the volume of the release box required by the anesthetized male mosquitoes is small.
E. Before release, the packed release box is transported to a release place to be released, the male mosquitoes can be transported by adopting a refrigeration house vehicle or placed in the refrigeration house, and the temperature of the refrigeration house vehicle or the refrigeration house is set to be 10 ℃;
F. as shown in table 1, the average recovery rate of the male mosquitoes after the male mosquitoes are refrigerated for 3 hours under the condition of 10 degrees is 93.8%, the average recovery time is 1.3 to 1.5 minutes, the average recovery rate of the male mosquitoes after the male mosquitoes are refrigerated for 24 hours under the condition of 10 degrees is 88.7%, the average recovery time is 1.3 to 1.5 minutes, and the shorter the refrigeration time of the male mosquitoes under the same temperature is, the shorter the time of the male mosquitoes from the anesthesia state to the recovery of the flight ability at room temperature is, the higher the recovery rate is.
The advantages of this embodiment over the first to fourth embodiments are: compared with the first embodiment and the second embodiment, in this embodiment, the time taken for the male mosquito to recover the flight ability from the anesthesia state at room temperature is short, and the recovery speed is fast.
To sum up, the relationship between the temperature and the time in the refrigerator is controlled to avoid affecting the recovery rate of the anesthetized male mosquitoes during cold storage, and the five examples and table 1 are combined to balance the conditions of the temperature, the recovery time and the recovery rate, and the optimal method is to put the male mosquitoes into the refrigerator with the temperature of 1 ℃ for cold storage for 2.5 to 5.5 minutes to rapidly enter the anesthetized state, move the male mosquitoes into the refrigerator with the temperature of 10 ℃ for packaging and storage after all the male mosquitoes enter the anesthetized state, and meanwhile, keep the male mosquitoes transported at the temperature of 10 ℃.
TABLE 1
The above are merely specific embodiments of the present invention, and the scope of the present invention is not limited thereby; any alterations and modifications without departing from the spirit of the invention are within the scope of the invention.
Claims (7)
1. The method for releasing the mosquitoes through refrigeration anesthesia transportation is characterized by comprising the following steps:
A. loading male pupae into an emergence barrel or an emergence cage, adding water into the emergence barrel or the emergence cage, and allowing the male pupae to naturally emerge into male mosquitoes in the emergence barrel or the emergence cage; the density of the male pupae in the eclosion barrel or the eclosion cage is 15 to 25 per cubic centimeter;
B. draining water in the eclosion barrel or the eclosion cage, naturally drying the eclosion barrel or the eclosion cage and the male mosquitoes, and simultaneously adjusting the density of the male mosquitoes in the eclosion barrel or the eclosion cage to be 0.16 to 0.2 per cubic centimeter;
C. placing the eclosion barrel or the eclosion cage into a first refrigeration house for refrigeration, and enabling the male mosquitoes to enter an anesthesia state;
D. transferring the anesthetized male mosquitoes to a second refrigeration house, and placing the anesthetized male mosquitoes in a release box in the second refrigeration house for packaging;
E. transporting the release cartridge to a release location for release;
F. the released male mosquitoes rapidly recover flight ability at room temperature.
2. A method for releasing cold storage and transportation of mosquitoes and insects as recited in claim 1, wherein in the step C, the temperature of the first refrigerator is 1 to 10 degrees.
3. A method for transporting and releasing cold-stored mosquitoes for anesthesia as claimed in claim 2, wherein in step C, the average time required for the male mosquitoes to enter the anesthesia state from the wake state is 2 minutes to 14 minutes.
4. A method for releasing cold storage and transportation of mosquitoes and insects as recited in claim 3, wherein in the step D, the temperature in the second refrigerator is 1 to 10 degrees.
5. A method for releasing cold storage and transportation of mosquitoes and insects as recited in claim 4, wherein in the step D, the temperature of the second cold storage is 5 to 10 degrees.
6. The method for delivering cold-stored mosquito and insect anesthesia of claim 5, wherein in step E, the temperature of the delivery box during delivery is 1 to 10 degrees.
7. The method for delivering cold-stored mosquito and insect anesthesia of claim 6, wherein in step E, the temperature of the delivery box during delivery is 5 to 10 degrees.
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| CN107396891A (en) * | 2017-08-22 | 2017-11-28 | 中国农业科学院植物保护研究所 | A kind of method that constant low temperature stores the pale yellow female pupa of grace aphid chalcid fly |
| CN107372370B (en) * | 2017-09-08 | 2020-08-18 | 广州威佰昆生物科技有限公司 | Hatching solution for hatching aedes albopictus eggs and hatching method for aedes albopictus eggs |
| CN107568202B (en) * | 2017-10-17 | 2020-09-11 | 广州威佰昆生物科技有限公司 | Method for delaying eclosion of aedes albopictus mosquito pupae and method for transporting aedes albopictus mosquito pupae |
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