CN108575485A - The mould application and method of administration in prevention citrus root rot of Moses's pipe handle capsule - Google Patents
The mould application and method of administration in prevention citrus root rot of Moses's pipe handle capsule Download PDFInfo
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- CN108575485A CN108575485A CN201810229206.XA CN201810229206A CN108575485A CN 108575485 A CN108575485 A CN 108575485A CN 201810229206 A CN201810229206 A CN 201810229206A CN 108575485 A CN108575485 A CN 108575485A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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Abstract
The mould method of administration in prevention citrus root rot of Moses's pipe handle capsule, including：S1, it takes in culture substrate to cultivating container, meanwhile, take the mould layering of Moses's pipe handle capsule to be seeded in culture substrate；S2, Fructus Aurantii is taken to grow directly from seeds transplantation of seedlings in step S1 cultivating containers；S3, the Fructus Aurantii seedling transplanted is positioned in glasshouse；Plant is harvested after S4, cultivation management, and citrus Pathogens Causing Root Rot Disease is inoculated with before harvest.Its advantage is that：Improve plant leaf blade and root system salicylic acid, calmodulin contents；Plant leaf blade jasmonic, content of nitric oxide are improved, root system jasmonic, content of nitric oxide are reduced；Because salicylic acid, jasmonic, carbon monoxide, calmodulin are signal transduction substances in plant, when plant is by pathogen infection, can this stimulation be passed to intracellular and then plant is made to make corresponding stress reaction, to resist pathogen infection, these four substances are accumulated in plant illustrates that arbuscular mycorrhiza can improve the resistance to root rot by improving the content of this four kinds of substances.
Description
Technical field
The present invention relates to plant resistance to environment stress technical fields, and in particular to a kind of Moses's pipe handle capsule is mould to prevent citrus root rot
Using and method of administration.
Background technology
Citrus is one of most important industrial crops in the whole world, is the important fruit tree in China, China's citrus cultivated area and production
Amount ranks first in the world.Citrusfruit soft and succulency, sweet and sour taste are liked by numerous people.But it is planted in production
In the process, citrus is also faced with many disease pest harm, and wherein root rot is because its occurring area is wide, harmfulness is big, cures the period
It is long, and as the major obstacle of citrus stable high yield, larger economic loss is brought to Citrus Industry.
Citrus root rot also known as foot rot, skirt maize ear rot are a kind of soil-borne diseases endangering citrus rootstock and root,
There is generation in each citrus producing region in China.Root rot susceptible initial stage disease portion bark is in irregular web rot shape, and color browning often flows out brown
Coloring agent liquid has vinasse smell.Disease extends to xylem, and xylem discoloration is made to rot, and the strong boundary of disease is apparent.Morbidity later stage bark
Dry and cracked to fall off, xylem is exposed.It is generally acknowledged that the cause of disease of citrus root rot is caused to be that Phytophthora (Phytophthora spp) is true
Bacterium, pathogenic has phytophthora parasitica (P.parasitica), Papilio xuthus Linnaeus (P.citrophthora), palm mould
(P.palmvora), Phytophthora cactorum (P.cactorum), Phytophthora capsici (P.capsici) and Phytophthora nicotianae (P.nicotianae)
Deng.In addition, sickle-like bacteria (Fusarium spp) can promote infecting for phytophthora, accelerate the generation of root rot.Studies have shown that parasitic
Phytophthora is the most important pathogenic bacteria of China's citrus root rot.
Arbuscular mycorrhizal fungi is a kind of soil fungi distributed more widely in soil, terrestrial plant (packet that can be with about 90%
Include citrus) root system formation mutualistic symbiosis body --- arbuscular mycorrhiza.The few even nothing of Citrus Root root hair, Cong Zhi under the conditions of field cultivation
The function of mycorhiza partial alternative root hair, therefore, citrus is higher to the dependence of arbuscular mycorrhiza.How to be carried using arbuscular mycorrhizal fungi
High citrus is extremely urgent to the resistance of root rot.
Invention content
In order to solve above-mentioned technological deficiency, the method provided by the present invention is simple, a kind of effect Moses's pipe handle capsule outstanding it is mould
Prevent application and the method for administration of citrus root rot.
One aspect of the present invention protects the mould application in prevention citrus root rot of Moses's pipe handle capsule；
Preferably, it is to be seeded to Trifolium repense root by Moses's pipe handle capsule is mould using Trifolium repense as host plant that Moses's pipe handle capsule is mould
System, the root segment for expanding the matrix of numerous Trifolium repense root system collected after the completion and infecting.
On the other hand the mould method of administration in prevention citrus root rot of protection Moses's pipe handle capsule, includes the following steps：
S1, it takes in culture substrate to cultivating container, meanwhile, take the mould layering of Moses's pipe handle capsule to be seeded in culture substrate；
S2, Fructus Aurantii is taken to grow directly from seeds in transplantation of seedlings cultivating container described in step S1；
S3, it the Fructus Aurantii seedling transplanted is positioned in glasshouse carries out cultivation management, the daily 100- that waters per basin
200mL；
S4, cultivation management harvest plant after 90-110 days, and in first 7 days inoculation citrus Pathogens Causing Root Rot Diseases of harvest.
The beneficial effects of the invention are as follows：Method is simple to operation, using rear significant effect, rationally, has efficiently played disease
Arbuscular mycorrhiza includes to the resistance of citrus root rot, specific effect under opportunistic pathogen infection condition：
(1) it effectively facilitates citrus plant vegetative growth, improve biomass；Promote the development of root system configuration, it is long to increase main root
Degree and lateral root numbers at different levels；Plant leaf blade and root system beta-1,3-glucanase and chitinase activity and total solvable are improved simultaneously
Property phenol and content of lignin, to achieve the purpose that anti-root rot；
(2) plant leaf blade and root system salicylic acid content are improved；Plant leaf blade jasmine acid content is improved, root system jasmonic is reduced
Content；Plant leaf blade content of nitric oxide is improved, root system content of nitric oxide is reduced；It improves plant leaf blade and root system calmodulin contains
Amount, because salicylic acid, jasmonic, carbon monoxide, calmodulin are signal transduction substances in plant, in plant by pathogen infection
When, can this stimulation be passed to intracellular and then plant is made to make corresponding stress reaction, to resist pathogen infection, because
This, these four substances are accumulated in plant illustrates that arbuscular mycorrhiza can be improved by improving the content of this four kinds of substances to root-rot
The resistance of disease；
(3) root rot morbidity main portions plant rootstock, therefore, in method of administration of the present invention, in step S4
By being inoculated at the rootstock of seedling, reinforce accuracy of causing a disease；
(4) the method for the present invention is simple, and production cost is low, and the yield of mandarin tree, additional income can be improved.
Specific implementation mode
The mould method of administration in prevention citrus root rot of Moses's pipe handle capsule, includes the following steps：
S1, it takes in culture substrate to cultivating container, meanwhile, take the mould layering of Moses's pipe handle capsule to be seeded in culture substrate；
Preferably, culture substrate described in step S1 is soil and husky mixture, and soil and husky volume ratio are 5:1；
Further, the soil was the sterilized soil of 6mm sieves, described husky for sterilizing sand.
Further, the sterilized soil and husky sterilising conditions are 121 DEG C, and sterilize 1h under the conditions of 0.11MPa.
Further, every basin Moses's pipe handle capsule mould species and the mass ratio of matrix are 1:16.
Preferably, inocalation method is layered described in step S1 is specially：One layer of culture substrate is first spread in a reservoir, inoculates one layer
Moses's pipe handle capsule is mould, covers culture substrate；Then it is mould to inoculate one layer of Moses's pipe handle capsule, covers culture substrate, and twice successively
The mould mass ratio of Moses's pipe handle capsule of inoculation is 2:1.
S2, Fructus Aurantii is taken to grow directly from seeds in transplantation of seedlings cultivating container described in step S1；
Preferably, Fructus Aurantii seedling described in step S2 is the illumination training in 28/20 DEG C of day night temperature, relative humidity 80%
Support the seedling (seedling with 5 true leaves and in the same size) of vernalization 4 weeks in case；
Further, the Fructus Aurantii kind full grains in the same size of vernalization are used for, and the NaOH through 1M to wash away pectin before vernalization,
70% ethyl alcohol surface sterilization 10min, deionized water rinse 4 times after reseed in advance by high pressure steam sterilization (0.11MPa,
121 DEG C) sand bed in.
S3, it the Fructus Aurantii seedling transplanted is positioned in glasshouse carries out cultivation management, the daily 100- that waters per basin
200mL；
Preferably, glasshouse primary condition described in step S3 is：Photosynthetic photon flux density is 338-982 μ
mol/m2/s；Diurnal temperature is 20-35/15-26 DEG C；Relative air humidity is 70-95%.
S4, cultivation management harvest plant after 100-110 days, and in first 7 days inoculation citrus Pathogens Causing Root Rot Diseases of harvest；
Preferably, the inoculation method of Pathogens Causing Root Rot Disease described in step S4 is：The last week is harvested in citrus seedling, it will be real
Raw seedling is cleaned from native face 1-2cm rootstocks with clear water, then carries out sterilization treatment with 75% alcohol, then uses aseptic water washing 2~3
Time；Then, on flame the blade of calcination cut longitudinal wound that size is about 5mm, and by activated root rot disease
Opportunistic pathogen is inoculated on wound, covers the absorbent cotton moisturizing of sterilizing, then is wrapped up with preservative film, drips 1-2mL sterile waters daily later,
Cotton balls is set to keep moisture state；
Further, the Pathogens Causing Root Rot Disease is phytophthora parasitica；
Further, the phytophthora parasitica cultivates 6 days phytophthora parasitica mycelia blocks on PDA plate under the conditions of being 28 DEG C.
The present invention is further described below in conjunction with specific embodiment.The embodiments described below is exemplary
, it is only used for explaining the present invention, and be not considered as limiting the invention.
Experimental method in following embodiments is unless otherwise specified conventional method.Reality as used in the following examples
It tests material unless otherwise specified, is that market is commercially available.
1, experiment material
For trying plant：Citrus often uses stock trifoliate orange (Poncirus trifoliata L.Raf.)；
For trying arbuscular mycorrhizal fungi：The mould Funneliformis mosseae (F.m) of Moses's pipe handle capsule, propagation method are：
Take Changjiang University's root system biological study F.m strains be seeded to Trifolium repense root system using Trifolium repense as host plant, 16 weeks
Afterwards, F.m expands numerous completion, then collects the root system and matrix of Trifolium repense, and F.m strains used mainly exist in the form of matrix；
For trying Pathogens Causing Root Rot Disease：Phytophthora parasitica Phytophthora parasitica (P.p), propagation method are：It takes
The edge inoculated by hypha block of phytophthora parasitica bacterium colony is central to PDA plate, cultivates 6d under the conditions of 28 DEG C, cuts edge mycelia block and be used for
Pathogen is inoculated with, and mycelia block size is 5 × 5mm.
2, experiment process
This experiment is designed using 2 × 2 dual factors tests, and factor one, which is that Moses's pipe handle capsule is mould, to be inoculated with and be not inoculated with, factor two
It is inoculated with and is not inoculated with for phytophthora parasitica.
Experiment set not Arbuscular Mycorrhizal Fungi and be not inoculated with phytophthora parasitica (- F.m-P.p), Arbuscular Mycorrhizal Fungi but
It is not inoculated with phytophthora parasitica (+F.m-P.p), not Arbuscular Mycorrhizal Fungi but is inoculated with phytophthora parasitica (- F.m+P.p), is both inoculated with clump
Mycorrhizal fungi is inoculated with 4 processing of phytophthora parasitica (+F.m+P.p) again, and each processing is repeated 5 times, and is 1 repetition per basin, per basin
3 plants of Fructus Aurantii seedlings are transplanted, totally 20 basin.Experiment basin is placed in Changjiang University's gardening gardens institute Mini-Scape Garden glasshouse at random
It is interior.
On March 18th, 2017 strips out trifoliate orange kind from trifoliate orange fruit, the trifoliate orange kind of full grains in the same size is chosen, through 1M's
NaOH washes away pectin, 70% ethyl alcohol surface sterilization 10min, and deionized water is seeded in after rinsing 4 times goes out by high steam in advance
In the sand bed of bacterium (121 DEG C, 0.11MPa).By sand bed be placed in the incubator of 28/20 DEG C of day night temperature, relative humidity 80% into
Row vernalization.
On April 8th, 2017 chooses 5 true leaves and Trifoliate seedling of the same size is transplanted.Cultivation matrix is per basin
1.5kg, for the mixture of soil and sand through high pressure steam sterilization (121 DEG C, 0.11MPa, 1h), the two mass ratio is 5:1.Experiment
It is 1.5L white plastic basins with basin, specification is：Upper bore 13.5cm, lower relative aperture 9.5cm, high 11cm.
The mould inoculation work of Moses's pipe handle capsule is carried out when transplanting, using layering inocalation method.One layer of 5cm high is first spread in plastic tub
The cultivation matrix of degree, then one layer of Moses's pipe handle capsule of inoculation is mould, and the mould dosage of Moses's pipe handle capsule is 60g (1500 spores at this time
Son).Then the cultivation matrix that a layer height is 3cm is covered, it is mould to inoculate one layer of Moses's pipe handle capsule, and Moses's pipe handle capsule is mould at this time
Dosage be 30g (750 spores).It takes above-mentioned Trifoliate seedling to be transplanted at this time, finally covers the culture substrate of 3cm height.
It is positioned over after sprinkling profoundly water in Changjiang University's glasshouse and carries out cultivation management, the daily 100mL that waters per basin.Glasshouse it is basic
Condition is：Photosynthetic photon flux density is 338-982 μm of ol/m2/s；Diurnal temperature is 20-35/15-26 DEG C；Air
Relative humidity is 70-95%.
On July 12nd, 2017 is inoculated with Pathogens Causing Root Rot Disease phytophthora parasitica.Specific method is：By seedling from native face 1cm roots
It is cleaned with clear water at neck, then sterilization treatment is carried out with 75% alcohol, then use aseptic water washing 2 times.Then, it is burnt on flame
The blade burnt cuts longitudinal wound that size is about 5mm, and activated Pathogens Causing Root Rot Disease is inoculated on wound, covers
The absorbent cotton moisturizing of sterilizing is covered, then is wrapped up with preservative film.It drips 2mL sterile waters daily later, cotton balls is made to keep moisture state.2017
On July 19, in harvests plant.3, result
(1), Mycorrhizal Infection Incidence is measured using trypan blue decoration method (Phillips and Hayman, 1970), is surveyed with tape measure
Singling is high, measures stem thickness with vernier caliper, artificial counting obtains the number of blade, and it is fresh with underground part that electronic scale weighs overground part fresh weight
Weight, testing result are as shown in table 1 below：
Trifoliate seedling Mycorrhizal Infection Incidence, plant height, stem thickness, the number of blade, aerial part and underground part under the conditions of 1 different disposal of table
Divide fresh weight
Note：The different lowercases of same column data (mean+SD, n=4) are (between a, b, c and d) expression processing
There are significant difference (P<0.05).Similarly hereinafter.
By 1 result of upper table it is found that pathogen infection to significantly reduce Trifoliate seedling plant height, stem thickness, the number of blade, overground part fresh
Weight and underground part fresh weight, show particularly evident in Arbuscular Mycorrhizal Fungi plant.No matter in addition, whether pathogen infection,
Arbuscular Mycorrhizal Fungi all significantly promotes plant growth.By in the plant of pathogen infection, Arbuscular Mycorrhizal Fungi is not true
Bacterium significantly improves Trifoliate seedling plant height, stem thickness, the number of blade, overground part fresh weight and underground part fresh weight, respectively 60%, 17%,
35%, 63% and 36%.Under the conditions of pathogen infection, Arbuscular Mycorrhizal Fungi significantly improves Trifoliate seedling plant height, stem
Slightly, the number of blade, overground part fresh weight and underground part fresh weight, respectively 37%, 17%, 25%, 61% and 9%.
(2), using WinRHIZO root scanners acquisition root system configuration relevant parameter, (system general length, root system are average straight
Diameter, root system projected area, root system surface area, root system volume etc.), testing result is as shown in table 2 below：
The total length of Trifoliate seedling root system under the conditions of 2 different disposal of table, projected area, surface area, root average diameter and
Root system volume
By 2 result of upper table it is found that pathogen infection inhibits the development of Trifoliate seedling root system configuration, in Arbuscular Mycorrhizal Fungi
It is showed in fungi plant particularly evident.No matter but whether pathogen infection, Arbuscular Mycorrhizal Fungi all significantly promotes trifoliate orange
The development of seedling root system configuration.By in the plant of pathogen infection, Arbuscular Mycorrhizal Fungi does not significantly improve the dried immature fruit of citron orange
Raw seedling system general length, projected area, surface area and volume, have increased separately 47%, 55%, 79% and 47%.In pathogen
Under infection condition, Arbuscular Mycorrhizal Fungi significantly improves Trifoliate seedling system general length, projected area, surface area and body
Product, has increased separately 10%, 17%, 35% and 14%.
(3), manually statistics level-one lateral root, the quantity of two level lateral root and three-level lateral root, testing result are as shown in table 3 below：
The quantity of Trifoliate seedling main root length and level-one lateral root, two level lateral root and three-level lateral root under the conditions of 3 different disposal of table
By 3 result of upper table it is found that pathogen infection reduces Trifoliate seedling main root length and lateral root number at different levels, it is being inoculated with
It is showed in arbuscular mycorrhizal fungi plant particularly evident.No matter but whether inoculation pathogen, Arbuscular Mycorrhizal Fungi is all notable
Improve Trifoliate seedling main root length and two level lateral root, three-level lateral root number.By in the plant of pathogen infection, clump be not inoculated with it
Mycorrhizal fungi significantly improves Trifoliate seedling main root length and two level lateral root, three-level lateral root number, be respectively increased 47%,
53% and 80%.Under the conditions of pathogen infection, Arbuscular Mycorrhizal Fungi significantly improves Trifoliate seedling main root length and two
Grade lateral root, three-level lateral root number, have been respectively increased 10%, 19% and 34%.
(4), with reference to the method for Lou Shubao (2008) and the method for Rui Haiying (2008), to the dried immature fruit of citron orange under the conditions of different disposal
β -1 in the blade and root system of raw seedling, 3- dextranase activities and chitinase activity are detected, testing result such as the following table 4 institute
Show：
Beta-1,3-glucanase and chitinase activity in Trifoliate seedling blade and root system under the conditions of 4 different disposal of table
It can be obtained by 4 result of upper table, pathogen infection reduces β -1 in Trifoliate seedling blade and root system, 3- glucan enzyme activity
Property and chitinase activity, show particularly evident in Arbuscular Mycorrhizal Fungi plant.No matter in addition, inoculation pathogen with
No, Arbuscular Mycorrhizal Fungi all significantly improves β -1 in Trifoliate seedling blade and root system, 3- dextranase activities and chitin
Enzymatic activity.Not by the plant of pathogen infection, it is poly- that Arbuscular Mycorrhizal Fungi significantly improves the Portugals Trifoliate seedling β -1,3-
Anase activity and chitinase activity have been respectively increased 8% and 29% in blade, 13% and 65% have been respectively increased in root system.
Under the conditions of pathogen infection, Arbuscular Mycorrhizal Fungi significantly improves Trifoliate seedling β -1,3- dextranase activity and chitin
Matter enzymatic activity has been respectively increased 15% and 31% in blade, 8% and 49% has been respectively increased in root system.
(5), with reference to the method for (2017) Zhang etc., to the total soluble phenolics and wood of Trifoliate seedling under the conditions of different disposal
Lignin content is detected, and testing result is as shown in table 5 below：
Total soluble phenolics and content of lignin in Trifoliate seedling blade and root system under the conditions of 5 different disposal of table
It can be obtained by 5 result of upper table, pathogen infection reduces in Trifoliate seedling blade and root system total soluble phenolics and wooden
Cellulose content.No matter but whether inoculation pathogen, Arbuscular Mycorrhizal Fungi all improves total in Trifoliate seedling blade and root system
Soluble phenolics and content of lignin show particularly evident in root system.It in the plant of pathogen infection, be not inoculated with clump branch bacterium
Mycorrhiza fungi significantly improves the total soluble phenolics of Trifoliate seedling and content of lignin, and 7% and 9% have been respectively increased in blade,
19% and 10% have been respectively increased in root system.Under the conditions of pathogen infection, Arbuscular Mycorrhizal Fungi significantly improves the dried immature fruit of citron orange
The total soluble phenolics of raw seedling root system and content of lignin, have been respectively increased 22% and 14%, and to total soluble phenolics of blade and wooden
Cellulose content has no significant effect.
(6), using enzyme-linked immunization to salicylic acid and jasmonic in Trifoliate seedling blade under the conditions of different disposal and root system
Content is detected, and testing result is as shown in table 6 below：
Salicylic acid and jasmine acid content in Trifoliate seedling blade and root system under the conditions of 6 different disposal of table
It can be obtained by 6 result of upper table, pathogen infection reduces salicylic content in Trifoliate seedling blade and root system, is connecing
It is showed in kind arbuscular mycorrhizal fungi plant particularly evident.No matter but whether inoculation pathogen, Arbuscular Mycorrhizal Fungi all carries
Salicylic acid content in high Trifoliate seedling blade and root system.Not by the plant of pathogen infection, Arbuscular Mycorrhizal Fungi
Salicylic acid content in Trifoliate seedling blade and root system is significantly improved, 63% and 33% has been respectively increased.In pathogen infection item
Under part, Arbuscular Mycorrhizal Fungi significantly improves salicylic acid content in Trifoliate seedling blade and root system, has been respectively increased 28%
With 55%.
Pathogen infection reduces Trifoliate seedling root system jasmine acid content, but improves blade jasmine acid content.In addition, not
Whether by inoculation pathogen, Arbuscular Mycorrhizal Fungi all improves Trifoliate seedling blade jasmine acid content, but reduces root system
Jasmine acid content.By in the plant of pathogen infection, Arbuscular Mycorrhizal Fungi does not cause blade jasmine acid content to increase
178%, and root system jasmine acid content reduces 43%.Under the conditions of pathogen infection, Arbuscular Mycorrhizal Fungi leads to blade
Jasmine acid content increases 99%, and root system jasmine acid content reduces 36%.
(7), using double antibody sandwich method to nitric oxide and calcium in Trifoliate seedling blade under the conditions of different disposal and root system
Cellulose content is adjusted to be detected, testing result is as shown in table 7 below：
Nitric oxide and calmodulin contents in Trifoliate seedling blade and root system under the conditions of 7 different disposal of table
It can be obtained by 7 result of upper table, pathogen infection significantly increases nitric oxide production in Trifoliate seedling blade and root system contain
Amount, no matter and inoculation pathogen whether, Arbuscular Mycorrhizal Fungi all significantly increases blade content of nitric oxide, but reduces
Root system content of nitric oxide.By in the plant of pathogen infection, Arbuscular Mycorrhizal Fungi does not cause blade nitric oxide to contain
Amount increases 47%, and root system content of nitric oxide reduces 19%.Under the conditions of pathogen infection, Arbuscular Mycorrhizal Fungi
Blade content of nitric oxide is caused to increase 46%, and root system content of nitric oxide reduces 11%.
Pathogen infection reduces the content of calmodulin in Trifoliate seedling blade and root system, is planted in Arbuscular Mycorrhizal Fungi
It is showed in strain particularly evident.However, whether pathogen infection, Arbuscular Mycorrhizal Fungi all significantly increases Trifoliate seedling leaf
The content of calmodulin in piece and root system.By in the plant of pathogen infection, Arbuscular Mycorrhizal Fungi does not significantly improve trifoliate orange
Grow directly from seeds calmodulin contents in seedling leaf and root system, 13% and 17% has been respectively increased.Under the conditions of pathogen infection, inoculation clump branch
Mycorrhizal fungi significantly improves calmodulin contents in Trifoliate seedling blade and root system, and 9% and 4% has been respectively increased.
In conclusion the mould raising citrus of inoculating microbes Moses pipe handle capsule of the present invention to the resistance of root rot be mainly manifested in
Lower aspect：Promote citrus plant vegetative growth, improve biomass；Promote the development of root system configuration, increases main root length and at different levels
Lateral root number；Improve plant leaf blade and root system beta-1,3-glucanase and chitinase activity；It improves plant leaf blade and root system is total
Soluble phenolics and content of lignin；Improve plant leaf blade and root system salicylic acid content；Plant leaf blade jasmine acid content is improved, is reduced
Root system jasmine acid content；Plant leaf blade content of nitric oxide is improved, root system content of nitric oxide is reduced；Improve plant leaf blade and root
It is calmodulin contents.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Claims (10)
1. the mould application in prevention citrus root rot of Moses's pipe handle capsule.
2. the mould application in prevention citrus root rot of Moses's pipe handle capsule as described in claim 1, it is characterised in that：Moses's pipe handle
It is to be seeded to Trifolium repense root system by Moses's pipe handle capsule is mould using Trifolium repense as host plant that capsule is mould, expand it is numerous after the completion of collect white three
The matrix of blade root system and the root segment infected.
3. a kind of mould method of administration in prevention citrus root rot of Moses's pipe handle capsule according to claim 1 or claim 2, feature exist
In：Include the following steps
S1, it takes in culture substrate to cultivating container, meanwhile, take the mould layering of Moses's pipe handle capsule to be seeded in culture substrate；
S2, Fructus Aurantii is taken to grow directly from seeds in transplantation of seedlings cultivating container described in step S1；
S3, it the Fructus Aurantii seedling transplanted is positioned in glasshouse carries out cultivation management, the daily 100-200mL that waters per basin；
S4, cultivation management harvest plant after 90-110 days, and in first 7 days inoculation citrus Pathogens Causing Root Rot Diseases of harvest.
4. the mould method of administration in prevention citrus root rot of Moses's pipe handle capsule according to claim 3, it is characterised in that：Step
Culture substrate described in S1 is soil and husky mixture, and soil and husky volume ratio are 5:1.
5. the mould method of administration in prevention citrus root rot of Moses's pipe handle capsule according to claim 3, it is characterised in that：Step
In S1, per basin Moses's pipe handle capsule mould species and the mass ratio of matrix is 1:16.
6. the mould method of administration in prevention citrus root rot of Moses's pipe handle capsule according to claim 3, it is characterised in that：Step
Inocalation method is layered described in S1 is specially：One layer of culture substrate is first spread in a reservoir, and it is mould to inoculate one layer of Moses's pipe handle capsule, covering training
Support matrix；Then it is mould to inoculate one layer of Moses's pipe handle capsule, covers culture substrate, and the mould matter of Moses's pipe handle capsule being successively inoculated with twice
Amount is than being 2:1.
7. the mould method of administration in prevention citrus root rot of Moses's pipe handle capsule according to claim 3, it is characterised in that：Step
Fructus Aurantii seedling described in S2 be 28/20 DEG C of day night temperature, relative humidity 80% illumination box in vernalization 4 weeks seedling.
8. the mould method of administration in prevention citrus root rot of Moses's pipe handle capsule according to claim 3, it is characterised in that：Step
Glasshouse primary condition described in S3 is：Photosynthetic photon flux density is 338-982 μm of ol/m2/s；Diurnal temperature is
20-35/15-26℃；Relative air humidity is 70-95%.
9. the mould method of administration in prevention citrus root rot of Moses's pipe handle capsule according to claim 3, it is characterised in that：Step
The inoculation method of Pathogens Causing Root Rot Disease described in S4 is：The last week is harvested in citrus seedling, by seedling from native face 1-2cm rootstocks
Place is cleaned with clear water, then carries out sterilization treatment with 75% alcohol, then uses aseptic water washing 2~3 times；Then, on flame
The blade of calcination cuts longitudinal wound that size is about 5mm, and activated Pathogens Causing Root Rot Disease is inoculated on wound,
The absorbent cotton moisturizing of sterilizing is covered, then is wrapped up with preservative film, drips 1-2mL sterile waters daily later, cotton balls is made to keep moisture state.
10. the mould method of administration in prevention citrus root rot of Moses's pipe handle capsule according to claim 9, it is characterised in that：Institute
It is phytophthora parasitica to state Pathogens Causing Root Rot Disease；And the phytophthora parasitica be 28 DEG C under the conditions of 6 days parasitic epidemic diseases are cultivated on PDA plate
Mould silk block.
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| CN110892845A (en) * | 2019-12-06 | 2020-03-20 | 长江大学 | Method for improving citrus fruit quality by inoculating arbuscular mycorrhizal fungi |
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