CN108410741A - A kind of obligate AM Inoculants of citrus and preparation method thereof - Google Patents
A kind of obligate AM Inoculants of citrus and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to agricultural plantation technology fields more particularly to a kind of obligate AM Inoculants of citrus and preparation method thereof.The present invention will be by that will detach the obligate AM fungies of citrus obtained according to 1：800 1000 ratio is mixed with sterile soil, and carries out rootstocks for citrus nursery 12 16 weeks wherein, make the obligate AM fungies of citrus adequately expanded it is numerous and with the abundant symbiosis of rootstocks for citrus root system, then will be packaged to be after rootstocks for citrus root system and its compost crushing.The present invention by strictly control the obligate AM fungies of citrus and sterile soil mixing ratio, and control the time of rootstocks for citrus nursery, it is high a kind of AM fungal contents have been finally obtained, inoculating active is good, and the wherein more suitable obligate AM fungies Inoculant of citrus of the outer mycelium of root, spore, root intramatrical mycelium, interior raw AM VA Mycorrhizal Fungis ratio.
Description
Technical field
The present invention relates to agricultural plantation technology fields more particularly to a kind of obligate AM Inoculants of citrus and preparation method thereof.
Background technology
AM fungies, i.e. arbuscular mycorrhizal fungi are a kind of important edaphons in Agro-ecological System, have to crop
Enhancing development improves nutrient absorption, improves many beneficial effects such as disease resistance and resistance.
Arbuscular mycorrhiza, 1885, German plant physiology and forest scholar Frank initiated " mycorhiza " (Fungus-root are
Mycorrhiza) this term.Mycorhiza is a kind of universal plant symbiosis phenomenon in nature, it is the mycorrhizal fungi in soil
A kind of association that mycelia is formed with higher plant nutrition root system.Symbiotic effects obtain necessary carbohydrate out of plant
And other nutriments, and plant also obtains required nutrition and moisture etc. from fungi there, to reach a kind of mutually beneficial mutual assistance,
The high unity exchanged each other's needs.Its not only feature with general root system of plant, but also the characteristic with obligate fungi.
Currently, external have relevant Inoculant sale, the factorial praluction technology of commercial preparation has tended to be ripe, but
Because it is expensive, inconvenient and existing in actual use problems are transported, cause the AM Inoculants of high quality also opposite
It is less.Currently, the country, which has also begun to relevant enterprise, sets foot in this field, but its product quality is irregular, and yield is small, purchase
After still need to further expand it is numerous.In general, domestic batch production in AM fungi Inoculants, commercially produce aspect and remained at
Step section, AM fungies cannot leave host plant and individually be cultivated in addition, therefore realize the extensive quotient of AM fungi Inoculants
The production of industry metaplasia is still more difficult.
A variety of improvement are carried out to the production technology of AM fungi Inoculants at this stage, as patent CN02159722.7 is disclosed
A method of bush mycorrhizal fungi preparation is produced, by 15~25 parts of careless carbon, 15~20 parts of vermiculite, 30~45 parts of perlite,
On the culture substrate of zeolite 15~25 (weight) part composition, inoculation is equivalent to the true containing arbuscular mycorrhiza of matrix volume 5~10%
The microbial inoculum of the brood body of bacterium mixes up mode using a variety of host plant mixed seeding and is cultivated；Pour one time of nutrition liquid within every 2~3 days；Training
After supporting 17~18 weeks, culture substrate and root system of plant are collected；Such as patent application CN201511023372.7, one kind is disclosed
Bush mycorrhizal fungi preparation is prepared according to the following steps：Using white clover seeds and Radix Ophiopogonis seed as host plant, with
Glomus mosseae, netted sacculus be mould, Glomus etunicatum or Glomus intraradices are Inoculant, is trained under the conditions of specific nutrition
It supports, the root for the plant for growing to harvest time is removed with upper bit, remaining root system and base portion crush mixing to get clump branch
Mycorrhizal fungi microbial inoculum, this two kinds of microbial inoculums contain various AM fungies, effective citrus obligate arbuscular mycorrhiza when for citrus plantation
Fungal content is less, and effect is poor.Therefore, it is current urgent problem to find a kind of obligate AM Inoculants of citrus.
Invention content
In order to solve the above technical problems existing in the prior art, the present invention provide a kind of obligate AM Inoculants of citrus and
Preparation method is achieved particular by following technical scheme：
(1) by sterile rootstocks for citrus seed by sterile warm water cleaning after, then with sterile warm water immersion 0.5-4h, use 65-
The rinsing of 85% alcohol is dried；
(2) by the obligate AM fungies of citrus according to 1：The ratio of 800-1000 is mixed with sterile soil, and cultivation wherein is dried
Rootstocks for citrus seed, carry out rootstocks for citrus nursery, make the obligate AM fungies of citrus adequately expanded it is numerous and with rootstocks for citrus root
It is abundant symbiosis；
(3) rootstocks for citrus root system and its compost are finally passed through pulverization process, make soil by rootstocks for citrus nursery 12-16 weeks
The brood bodies such as AM roots outer mycelium, spore in earth；Root intramatrical mycelium, interior raw AM VA Mycorrhizal Fungis in rootstocks for citrus root system, mixing
Packaging is to get the obligate AM fungies Inoculant of citrus after uniformly；
(4) control the obligate AM fungies Inoculant moisture 15-45% of citrus, packaging to get.
The rootstocks for citrus seed be trifoliate orange, citrange seed.
The obligate AM fungies are the fungal components that citrus can be made to generate arbuscular mycorrhiza.It is new that it can make citrus grow fasciation
Root expands new root quantity, effectively enhances tree vigo(u)r, improves the immune disease-resistance ability of tree, promotes Frankia and non-pulse family
Plant generates root nodule, forms Nitrogen Fixation System, increases soil fertility, increases the source canal of citrus intake nutrient in micro-ecological environment
Road.
The obligate AM fungies are to obtain and/or crush citrus group planting mycorhiza by being detached from soil and obtain.Contain in soil
There are the brood bodies such as AM roots outer mycelium, spore, contains root intramatrical mycelium and Nei Sheng AM VA Mycorrhizal Fungis in citrus group planting mycorhiza, it can be by
Acquisition need to be detached.
The temperature of the sterile warm water is 54-55 DEG C.At a temperature of this, rootstocks for citrus seed can obtain preferably activating effect
Fruit promotes bud ratio.
The sterile soil is by rural area soil, vermiculite, husk, plant ash according to rural area soil：Vermiculite：Husk：Plant ash=(3-
5):(0.5-1.5):(1.5-2.5):The weight ratio of (2-4) carries out mixture, then sterilize to get.It is obtained in this ratio sterile
Soil more grows suitable for obligate AM fungies and rootstocks for citrus.
The sterilizing is with 100 DEG C of steam treatment 2h or 121 DEG C of high pressure sterilization 30min.The overwhelming majority can be killed at this time
Harmful microorganism.
During rootstocks for citrus nursery, by VA Mycorrhizal Fungi sampling inspection, determine that AM bacterium are successfully inoculated with, and in stock under survival
Come.After obligate AM fungies are mixed with sterile soil, obligate AM fungies have Mortality, and the death rate is larger sometimes, cause not having
Obligate AM fungies survive in stock, influence the production success rate of the obligate AM fungies Inoculant of citrus.
A series of research and experiment are carried out in process of the present invention, following selected part process is disclosed：
Test example 1：Screening test is carried out to the proportioning of sterile soil：
It is for use that sterile soil is prepared according to following ratio：
| Rural area soil parts by weight | Vermiculite parts by weight | Husk parts by weight | Plant ash parts by weight | |
| 1st group | 4 | 1 | 2 | 3 |
| 2nd group | 5 | 0.5 | 1.5 | 2 |
| 3rd group | 3 | 1.5 | 1.5 | 2 |
| 4th group | 3 | 0.5 | 2.5 | 2 |
| 5th group | 3 | 0.5 | 1.5 | 4 |
| 6th group | 5 | 1 | 2 | 3 |
| 7th group | 4 | 1.5 | 2 | 3 |
| 8th group | 4 | 1 | 2.5 | 3 |
| 9th group | 4 | 1 | 2 | 4 |
| 10th group | 3 | 1 | 2 | 3 |
| 11st group | 4 | 0.5 | 2 | 3 |
| 12nd group | 4 | 1 | 1.5 | 3 |
| 13rd group | 4 | 1 | 2 | 2 |
13 parts of different sterile soils are obtained, are handled as follows：
(1) by the sterile rootstocks for citrus seeds of 30kg by sterile warm water cleaning after, then with sterile warm water immersion 2h, with 75%
Alcohol rinsing is dried, and is collected for use；
(2) by the obligate AM fungies of citrus respectively according to 1：900 ratio is mixed with each part sterile soil.
(3) by rootstocks for citrus seed difference nursery wherein 14 weeks, finally rootstocks for citrus root system and its compost are passed through
Pulverization process detects wherein spore density and Hyphal length after mixing.
| Group | Spore density (a/10g) | Mycelia total length (rice/10g) |
| 1st group | 138.2 | 180.6 |
| 2nd group | 87.3 | 126.3 |
| 3rd group | 91.4 | 137.5 |
| 4th group | 92.5 | 140.1 |
| 5th group | 88.6 | 139.6 |
| 6th group | 111.5 | 165.4 |
| 7th group | 117.8 | 167.6 |
| 8th group | 116.4 | 170.3 |
| 9th group | 120.4 | 173.7 |
| 10th group | 109.6 | 159.6 |
| 11st group | 102.4 | 168.6 |
| 12nd group | 105.6 | 165.2 |
| 13rd group | 103.4 | 163.7 |
By upper data it is found that suitable rural area soil in sterile soil：Vermiculite：Husk：Plant ash ratio can be promoted effectively
Spore density and mycelia total length, according to rural area soil：Vermiculite：Husk：Plant ash=4:1:2:The nothing that 3 weight ratio configures
Bacterium soil, the growth of the obligate AM fungies of citrus preferably.
Test example 2：Screening test is carried out to rootstocks for citrus seedling raise period：
Early-stage preparations are carried out as follows：
(2) by the sterile rootstocks for citrus seeds of 30kg by sterile warm water cleaning after, then with sterile warm water immersion 2h, with 75%
Alcohol rinsing is dried, and is collected for use；
(2) by rural area soil, vermiculite, husk, plant ash according to rural area soil：Vermiculite：Husk：Plant ash=4:1:2:3 weight
Then amount sterilizes than carrying out mixture, sterile soil is made, for use；
(3) by the obligate AM fungies of citrus respectively according to 1：700、1：800、1：900、1：1000、1：1100 ratio and nothing
Bacterium soil mixes, and obtains the obligate AM fungal cultures soil of 5 portions of different citruses.
The obligate AM fungal cultures soil of each citrus is equally divided into 5 groups.By rootstocks for citrus seed respectively nursery wherein 10,
12,14,16,18 weeks, rootstocks for citrus root system and its compost is finally passed through into pulverization process, detect its miospore after mixing
Density and Hyphal length.
By upper data it is found that promoting AM fungies and sterile soil ratio, the growth of spore density and Hyphal length can be promoted
Speed, but excessively high AM fungi ratios, can cause the spore density upper limit relatively low；And the rootstocks for citrus seed seedling-raising time exists
Spore density basically reaches peak value at 12-16 weeks, and continuing nursery can cause spore density to decline, and Hyphal length also not compared with
It is obviously improved.
Citrus root hair is less, stronger to the dependence of the group planting mycorrhizal fungi in soil, and group planting mycorrhizal fungi is total with citrus
Life can enhance the growth that root system promotes the absorption of moisture, mineral nutrition especially phosphorus plant.Arbuscular mycorrhiza expands citrus
Root system absorption region increases absorption of the root system to moisture and nutriment, improves the anti-adversity ability of plant, improves plant nutrient
Condition.Promote the growth of rhizosphere microorganism nitrogen-fixing bacteria, phosphobacteria, and has desirable influence to the dross of symbiotic nitrogen fixation microorganism.
The obligate AM fungies Inoculant of citrus can promote the growth of citrus root, and citrus is made to grow fasciation new root, expand new
Radical amount, effectively enhances tree vigo(u)r, improves the immune disease-resistance ability of tree, and Frankia is promoted to generate root with non-leguminous plant
Tumor forms Nitrogen Fixation System, increases soil fertility, increases the sources of citrus intake nutrient in micro-ecological environment, improves citrus
The ecological environment of growth has good adjuvant treatment effect to Citrus Huanglongbing pathogen.
Due to the property for the obligate symbiosis that AM fungies have, can not also individual sterile culture be carried out to it at present, it can only
It relies on live plant to breed it, there has been no preferable extensive AM fungies to be inoculated with agent and process for producing same.
The present invention will be by that will detach the obligate AM fungies of citrus obtained according to 1：The ratio of 800-1000 is mixed with sterile soil,
And rootstocks for citrus nursery 12-16 weeks is carried out wherein, so that the obligate AM fungies of citrus is adequately expanded numerous and and rootstocks for citrus
The abundant symbiosis of root system is packaged to be after then crushing rootstocks for citrus root system and its compost.The present invention is by strictly controlling mandarin orange
The ratio of the obligate AM fungies of tangerine and sterile soil mixing, and the time of rootstocks for citrus nursery is controlled, finally obtain a kind of AM fungies
Content is high, and inoculating active is good, and wherein root outer mycelium, spore, root intramatrical mycelium, interior raw AM VA Mycorrhizal Fungis equal proportion is more suitable
The obligate AM fungies Inoculant of suitable citrus.
Compared with prior art, the technique effect of the invention is embodied in：
The present invention will be by that will detach the obligate AM fungies of citrus obtained according to 1：The ratio of 800-1000 is mixed with sterile soil,
And rootstocks for citrus nursery 12-16 weeks is carried out wherein, so that the obligate AM fungies of citrus is adequately expanded numerous and and rootstocks for citrus
The abundant symbiosis of root system is packaged to be after then crushing rootstocks for citrus root system and its compost.The present invention is by strictly controlling mandarin orange
The ratio of the obligate AM fungies of tangerine and sterile soil mixing, and the time of rootstocks for citrus nursery is controlled, finally obtain a kind of AM fungies
Content is high, and inoculating active is good, and wherein the outer mycelium of root, spore, root intramatrical mycelium, interior raw AM VA Mycorrhizal Fungis ratio are more suitable
The obligate AM fungies Inoculant of citrus.
Specific implementation mode
It is limited with reference to specific embodiment technical scheme of the present invention is further, but claimed
Range describes made by being not only limited to.
Embodiment 1
A kind of obligate AM fungies Inoculant of citrus, is prepared via a method which：
(1) take the sterile trifoliate orange wood seeds of 1kg as rootstocks for citrus seed, sterile rootstocks for citrus seed is sterile by 54.5 DEG C
Warm water cleaning after, then with sterile warm water impregnate 2h, with 75% alcohol rinse dry；
(2) by the obligate AM fungies of citrus according to 1：900 ratio is mixed with sterile soil, cultivates the citrus anvil dried wherein
The wooden seed carries out rootstocks for citrus nursery, so that the obligate AM fungies of citrus is adequately expanded numerous and abundant with rootstocks for citrus root system
Symbiosis；
(3) rootstocks for citrus root system and its compost are finally passed through pulverization process, made in soil by rootstocks for citrus nursery 14 weeks
The brood bodies such as AM roots outer mycelium, spore；Root intramatrical mycelium, interior raw AM VA Mycorrhizal Fungis in rootstocks for citrus root system are uniformly mixed
After pack to get the obligate AM fungies Inoculant of citrus；
(4) control the obligate AM fungies Inoculant moisture 30% of citrus, packaging to get.
The obligate AM fungies obtain and crush citrus arbuscular mycorrhiza by being detached from soil and obtain, and can make
Citrus generates the fungal component of arbuscular mycorrhiza.
The sterile soil is obtained after common rural area soil sterilizes.
Embodiment 2
A kind of obligate AM fungies Inoculant of citrus, is prepared via a method which：
(1) it takes the sterile trifoliate orange wood seeds of 1kg as rootstocks for citrus seed, sterile rootstocks for citrus seed is passed through into 30 DEG C of sterile temperature
Water cleaning after, then with sterile warm water impregnate 0.5h, with 65% alcohol rinse dry；
(2) by the obligate AM fungies of citrus according to 1：800 ratio is mixed with sterile soil, cultivates the citrus anvil dried wherein
The wooden seed carries out rootstocks for citrus nursery, so that the obligate AM fungies of citrus is adequately expanded numerous and abundant with rootstocks for citrus root system
Symbiosis；
(3) rootstocks for citrus root system and its compost are finally passed through pulverization process, made in soil by rootstocks for citrus nursery 12 weeks
The brood bodies such as AM roots outer mycelium, spore；Root intramatrical mycelium, interior raw AM VA Mycorrhizal Fungis in rootstocks for citrus root system are uniformly mixed
After pack to get the obligate AM fungies Inoculant of citrus；
(4) control the obligate AM fungies Inoculant moisture 15% of citrus, packaging to get.
The obligate AM fungies obtain and crush citrus arbuscular mycorrhiza by being detached from soil and obtain, and can make
Citrus generates the fungal component of arbuscular mycorrhiza.
The sterile soil is obtained after common rural area soil sterilizes.
Embodiment 3
A kind of obligate AM fungies Inoculant of citrus, is prepared via a method which：
(1) it takes the sterile trifoliate orange wood seeds of 1kg as rootstocks for citrus seed, sterile rootstocks for citrus seed is passed through into 70 DEG C of sterile temperature
Water cleaning after, then with sterile warm water impregnate 4h, with 85% alcohol rinse dry；
(2) by the obligate AM fungies of citrus according to 1：1000 ratio is mixed with sterile soil, cultivates the citrus dried wherein
Stock seed carries out rootstocks for citrus nursery, so that the obligate AM fungies of citrus is adequately expanded numerous and filled with rootstocks for citrus root system
Divide symbiosis；
(3) rootstocks for citrus root system and its compost are finally passed through pulverization process, made in soil by rootstocks for citrus nursery 16 weeks
The brood bodies such as AM roots outer mycelium, spore；Root intramatrical mycelium, interior raw AM VA Mycorrhizal Fungis in rootstocks for citrus root system are uniformly mixed
After pack to get the obligate AM fungies Inoculant of citrus；
(4) control the obligate AM fungies Inoculant moisture 45% of citrus, packaging to get.
The obligate AM fungies obtain and crush citrus arbuscular mycorrhiza by being detached from soil and obtain, and can make
Citrus generates the fungal component of arbuscular mycorrhiza.
The sterile soil is by rural area soil, vermiculite, husk, plant ash according to rural area soil：Vermiculite：Husk：Plant ash=4:
1:2:3 weight ratio carries out mixture, then use 100 DEG C of steam treatment 2h to sterilize to get.
Embodiment 4
A kind of obligate AM fungies Inoculant of citrus, is prepared via a method which：
(1) take the sterile trifoliate orange wood seeds of 1kg as rootstocks for citrus seed, sterile rootstocks for citrus seed is sterile by 54.5 DEG C
Warm water cleaning after, then with sterile warm water impregnate 2h, with 75% alcohol rinse dry；
(2) by the obligate AM fungies of citrus according to 1：900 ratio is mixed with sterile soil, cultivates the citrus anvil dried wherein
The wooden seed carries out rootstocks for citrus nursery, so that the obligate AM fungies of citrus is adequately expanded numerous and abundant with rootstocks for citrus root system
Symbiosis；
(3) rootstocks for citrus root system and its compost are finally passed through pulverization process, made in soil by rootstocks for citrus nursery 14 weeks
The brood bodies such as AM roots outer mycelium, spore；Root intramatrical mycelium, interior raw AM VA Mycorrhizal Fungis in rootstocks for citrus root system are uniformly mixed
After pack to get the obligate AM fungies Inoculant of citrus；
(4) control the obligate AM fungies Inoculant moisture 30% of citrus, packaging to get.
The obligate AM fungies obtain and crush citrus arbuscular mycorrhiza by being detached from soil and obtain, and can make
Citrus generates the fungal component of arbuscular mycorrhiza.
The sterile soil is by rural area soil, vermiculite, husk, plant ash according to rural area soil：Vermiculite：Husk：Plant ash=4:
1:2:3 weight ratio carries out mixture, then use 100 DEG C of steam treatment 2h to sterilize to get.
Embodiment 5
A kind of obligate AM fungies Inoculant of citrus, is prepared via a method which：
(1) take the sterile trifoliate orange wood seeds of 1kg as rootstocks for citrus seed, sterile rootstocks for citrus seed is sterile by 54.5 DEG C
Warm water cleaning after, then with sterile warm water impregnate 2h, with 75% alcohol rinse dry；
(2) by the obligate AM fungies of citrus according to 1：900 ratio is mixed with sterile soil, cultivates the citrus anvil dried wherein
The wooden seed carries out rootstocks for citrus nursery, so that the obligate AM fungies of citrus is adequately expanded numerous and abundant with rootstocks for citrus root system
Symbiosis；
(3) rootstocks for citrus root system and its compost are finally passed through pulverization process, made in soil by rootstocks for citrus nursery 14 weeks
The brood bodies such as AM roots outer mycelium, spore；Root intramatrical mycelium, interior raw AM VA Mycorrhizal Fungis in rootstocks for citrus root system are uniformly mixed
After pack to get the obligate AM fungies Inoculant of citrus；
(4) control the obligate AM fungies Inoculant moisture 30% of citrus, packaging to get.
The obligate AM fungies obtain and crush citrus arbuscular mycorrhiza by being detached from soil and obtain, and can make
Citrus generates the fungal component of arbuscular mycorrhiza.
The sterile soil is by rural area soil, vermiculite, husk, plant ash according to rural area soil：Vermiculite：Husk：Plant ash=4:
1:2:3 weight ratio carries out mixture, then use 100 DEG C of steam treatment 2h to sterilize to get.
Embodiment 6
A kind of obligate AM fungies Inoculant of citrus, is prepared via a method which：
(1) take the sterile trifoliate orange wood seeds of 1kg as rootstocks for citrus seed, sterile rootstocks for citrus seed is sterile by 54.5 DEG C
Warm water cleaning after, then with sterile warm water impregnate 2h, with 75% alcohol rinse dry；
(2) by the obligate AM fungies of citrus according to 1：900 ratio is mixed with sterile soil, cultivates the citrus anvil dried wherein
The wooden seed carries out rootstocks for citrus nursery, so that the obligate AM fungies of citrus is adequately expanded numerous and abundant with rootstocks for citrus root system
Symbiosis；
(3) rootstocks for citrus nursery 14 weeks determine that AM bacterium are successfully inoculated with, and in stock during which by VA Mycorrhizal Fungi sampling inspection
In survive, rootstocks for citrus root system and its compost are finally passed through into pulverization process, make the AM roots in soil outer mycelium, spore
The brood bodies such as son；Root intramatrical mycelium, interior raw AM VA Mycorrhizal Fungis in rootstocks for citrus root system, are packed special to get citrus after mixing
Property AM fungi Inoculants；
(4) control the obligate AM fungies Inoculant moisture 30% of citrus, packaging to get.
The obligate AM fungies obtain and crush citrus arbuscular mycorrhiza by being detached from soil and obtain, and can make
Citrus generates the fungal component of arbuscular mycorrhiza.
Embodiment 7
A kind of obligate AM fungies Inoculant of citrus, is prepared via a method which：
(1) take the sterile trifoliate orange wood seeds of 1kg as rootstocks for citrus seed, sterile rootstocks for citrus seed is sterile by 54.5 DEG C
Warm water cleaning after, then with sterile warm water impregnate 2h, with 75% alcohol rinse dry；
(2) by the obligate AM fungies of citrus according to 1：900 ratio is mixed with sterile soil, cultivates the citrus anvil dried wherein
The wooden seed carries out rootstocks for citrus nursery, so that the obligate AM fungies of citrus is adequately expanded numerous and abundant with rootstocks for citrus root system
Symbiosis；
(3) rootstocks for citrus root system and its compost are finally passed through pulverization process, made in soil by rootstocks for citrus nursery 14 weeks
The brood bodies such as AM roots outer mycelium, spore；Root intramatrical mycelium, interior raw AM VA Mycorrhizal Fungis in rootstocks for citrus root system are uniformly mixed
After pack to get the obligate AM fungies Inoculant of citrus；
(4) control the obligate AM fungies Inoculant moisture 30% of citrus, packaging to get.
The obligate AM fungies are the symbiosis for being obtained by being detached from soil, and capable of making citrus generation arbuscular mycorrhiza
Bacterium.
Embodiment 8
A kind of obligate AM fungies Inoculant of citrus, is prepared via a method which：
(1) take the sterile trifoliate orange wood seeds of 1kg as rootstocks for citrus seed, sterile rootstocks for citrus seed is sterile by 54.5 DEG C
Warm water cleaning after, then with sterile warm water impregnate 2h, with 75% alcohol rinse dry；
(2) by the obligate AM fungies of citrus according to 1：900 ratio is mixed with sterile soil, cultivates the citrus anvil dried wherein
The wooden seed carries out rootstocks for citrus nursery, so that the obligate AM fungies of citrus is adequately expanded numerous and abundant with rootstocks for citrus root system
Symbiosis；
(3) rootstocks for citrus root system and its compost are finally passed through pulverization process, made in soil by rootstocks for citrus nursery 14 weeks
The brood bodies such as AM roots outer mycelium, spore；Root intramatrical mycelium, interior raw AM VA Mycorrhizal Fungis in rootstocks for citrus root system are uniformly mixed
After pack to get the obligate AM fungies Inoculant of citrus；
(4) control the obligate AM fungies Inoculant moisture 30% of citrus, packaging to get.
The obligate AM fungies are to be obtained by crushing citrus arbuscular mycorrhiza, and citrus can be made to generate arbuscular mycorrhiza
Fungal component.
Embodiment 9
A kind of obligate AM fungies Inoculant of citrus, is prepared via a method which：
(1) take the sterile citrange seeds of 1kg as rootstocks for citrus seed, sterile rootstocks for citrus seed is sterile by 54.5 DEG C
Warm water cleaning after, then with sterile warm water impregnate 2h, with 75% alcohol rinse dry；
(2) by the obligate AM fungies of citrus according to 1：900 ratio is mixed with sterile soil, cultivates the citrus anvil dried wherein
The wooden seed carries out rootstocks for citrus nursery, so that the obligate AM fungies of citrus is adequately expanded numerous and abundant with rootstocks for citrus root system
Symbiosis；
(3) rootstocks for citrus root system and its compost are finally passed through pulverization process, made in soil by rootstocks for citrus nursery 14 weeks
The brood bodies such as AM roots outer mycelium, spore；Root intramatrical mycelium, interior raw AM VA Mycorrhizal Fungis in rootstocks for citrus root system are uniformly mixed
After pack to get the obligate AM fungies Inoculant of citrus；
(4) control the obligate AM fungies Inoculant moisture 30% of citrus, packaging to get.
The obligate AM fungies obtain and/or crush citrus arbuscular mycorrhiza by being detached from soil and obtain, and energy
Citrus is set to generate the fungal component of arbuscular mycorrhiza.
Comparative example 1：It is made according to the embodiment 1 of patent CN02159722.7.
Comparative example 2：It is made according to the embodiment 1 of patent CN201511023372.7.
Apply contrast experiment in orchard：
It selects 12 pieces of non-conterminous kind of plot for being implanted with citrus in citrus orchard, 0.5 mu every piece, to apply embodiment 1- respectively
AM fungi Inoculants obtained by 9 and comparative example 1-2 stay one piece not apply as blank control group, each plot mandarin orange after measuring 4 months
The fungal infection rate of tangerine, current year orange yield and to current year produced citrus progress nutritive value measurement.
It is found that the obligate AM fungies Inoculant of citrus that the present invention obtains, fungal infection rate is high for comparison, to promoting orange yield
There is preferable effect, and can greatly promote the soluble solid of citrus and ascorbic content, also carrying slightly
It rises total sugar content and reduces total acid content, the flavor of citrus can be made further to be promoted.
Finally it is pointed out that above example is only the more representational example of the present invention.Obviously, technology of the invention
Scheme is not limited to above-described embodiment, and acceptable there are many deformations.Those skilled in the art can be from disclosed by the invention
All deformations that content is directly exported or associated, are considered as protection scope of the present invention.
Claims (9)
1. a kind of preparation method of the obligate AM Inoculants of citrus, which is characterized in that include the following steps：
(1) by sterile rootstocks for citrus seed by sterile warm water cleaning after, then with sterile warm water immersion 0.5-4h, use 65-85%
Alcohol rinsing is dried；
(2) by the obligate AM fungies of citrus according to 1：The ratio of 800-1000 is mixed with sterile soil, cultivates the citrus dried wherein
Stock seed carries out rootstocks for citrus nursery；
(3) rootstocks for citrus root system and its compost are finally passed through pulverization process by rootstocks for citrus nursery 12-16 weeks, are uniformly mixed
After pack to get the obligate AM fungies Inoculant of citrus；
(4) control the obligate AM fungies Inoculant moisture 15-45% of citrus, packaging to get.
2. the preparation method of the obligate AM Inoculants of citrus according to claim 1, which is characterized in that the rootstocks for citrus kind
Son be trifoliate orange, citrange seed.
3. the preparation method of the obligate AM Inoculants of citrus according to claim 1, which is characterized in that the obligate AM fungies
It is the fungal component that citrus can be made to generate arbuscular mycorrhiza.
4. the preparation method of the obligate AM Inoculants of citrus according to claim 1, which is characterized in that the obligate AM fungies
It is to obtain and/or crush citrus arbuscular mycorrhiza by being detached from soil and obtain.
5. the preparation method of the obligate AM Inoculants of citrus according to claim 1, which is characterized in that the sterile warm water
Temperature is 54-55 DEG C.
6. the preparation method of the obligate AM Inoculants of citrus according to claim 1, which is characterized in that the sterile soil be by
Rural area soil, vermiculite, husk, plant ash are according to rural area soil：Vermiculite：Husk：Plant ash=(3-5):(0.5-1.5):(1.5-
2.5):The weight ratio of (2-4) carries out mixture, then sterilize to get.
7. the preparation method of the obligate AM Inoculants of citrus according to claim 6, which is characterized in that the sterilizing is to use
100 DEG C of steam treatment 2h or 121 DEG C of high pressure sterilization 30min.
8. the preparation method of the obligate AM Inoculants of citrus according to claim 1, which is characterized in that rootstocks for citrus nursery stage
Between, by VA Mycorrhizal Fungi sampling inspection, determine that AM bacterium are successfully inoculated with, and survived in stock.
9. obligate by the citrus that the preparation method of the obligate AM Inoculants of claim 1-8 any one of them citruses is prepared
AM Inoculants.
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| CN111903412A (en) * | 2020-07-23 | 2020-11-10 | 广西壮族自治区农业科学院 | Method for producing citrus stock bitter orange mycorrhiza seedlings |
| CN111996124A (en) * | 2020-07-23 | 2020-11-27 | 广西壮族自治区农业科学院 | Leidectomyces flavus FS-1-106 and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110892845A (en) * | 2019-12-06 | 2020-03-20 | 长江大学 | Method for improving citrus fruit quality by inoculating arbuscular mycorrhizal fungi |
| CN111903412A (en) * | 2020-07-23 | 2020-11-10 | 广西壮族自治区农业科学院 | Method for producing citrus stock bitter orange mycorrhiza seedlings |
| CN111996124A (en) * | 2020-07-23 | 2020-11-27 | 广西壮族自治区农业科学院 | Leidectomyces flavus FS-1-106 and application thereof |
| CN111903412B (en) * | 2020-07-23 | 2022-02-22 | 广西壮族自治区农业科学院 | Method for producing citrus stock bitter orange mycorrhiza seedlings |
| CN111849785A (en) * | 2020-07-24 | 2020-10-30 | 广西壮族自治区农业科学院 | Saccharomycopsis tenuipes GZ-1 and application thereof |
| CN111849785B (en) * | 2020-07-24 | 2022-03-29 | 广西壮族自治区农业科学院 | Saccharomycopsis tenuipes GZ-1 and application thereof |
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