CN108558518A - A kind of mill water culture nutrient solution and its preparation and application improving soybean yields - Google Patents
A kind of mill water culture nutrient solution and its preparation and application improving soybean yields Download PDFInfo
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- CN108558518A CN108558518A CN201810570710.6A CN201810570710A CN108558518A CN 108558518 A CN108558518 A CN 108558518A CN 201810570710 A CN201810570710 A CN 201810570710A CN 108558518 A CN108558518 A CN 108558518A
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- nutrient solution
- sulfate
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- mill water
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C1/00—Ammonium nitrate fertilisers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/40—Fabaceae, e.g. beans or peas
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Hydroponics (AREA)
Abstract
The present invention relates to a kind of mill water culture nutrient solutions and its preparation and application improving soybean yields, it is necessary to coordinate Rhizobium Inoculation technology.The mill water culture nutrient solution is made of potassium nitrate, calcium nitrate, calcium chloride, ammonium nitrate, potassium sulfate, magnesium sulfate, magnesium chloride, manganese sulfate, ammonium sulfate, zinc sulfate, copper sulphate, ammonium molybdate, Boratex, cobalt chloride, EDTA molysite and potassium dihydrogen phosphate.Mill water culture nutrient solution of the present invention, in medium nitrogen level（2.4 mM）Under the conditions of, soybean yields can be significantly improved, it is more more effective than lift-augmented mechanism, it is a kind of low input high production, environmentally friendly plantation（Breeding）Pattern.Technical scheme of the present invention provides the nitrogen nutrition management mode that soybean yields is improved to give full play to biological nitrogen fixation to theoretical foundation, and has important guidance and practice significance to the development weight-reducing sustainable ecological agriculture of synergy.
Description
Technical field
The present invention relates to a kind of mill water culture nutrient solutions and its preparation and application improving soybean yields, belong to water planting nutrition
Liquid technical field.
Background technology
Nitrogen is one of macronutrient necessary to plant growth, be also the key that determine crop yield and quality because
Son.To alleviate pressure of the population growth to grain demand, China's amount of application of nitrogen fertilizer increases sharply.Due to a large amount of inputs of nitrogenous fertilizer,
The problems such as not only increasing agricultural cost, but also causing soil acidification, atmosphere pollution and body eutrophication (Guo et
al., 2010; Liu and Zhang 2011;Zhang et al., 2013), ecological environment is constituted and is seriously threatened.
Soybean is typical biological nitrogen fixation crop.The study found that the U.S. accounts for about the defeated nitrogen quantity in its farmland by the fixed nitrogen of legume
33%；In Brazil, soybean planting is under the conditions of low nitrogen or not nitrogen fertilizer application, by Rhizobium Inoculation, can also increase production 30% (Chen Wenxin
And Chen Wenfeng, 2004).It can be seen that giving full play to the biological nitrogen fixation of soybean, there is important meaning to agricultural weight-reducing synergy
Justice.
Limitation soybean biological nitrogen fixation it is many because being known as, and applying Namount number directly control nodule nitrogen fixation effect
Rate.The study found that low nitrogen can promote soybean nodulation, high nitrogen then to play inhibiting effect, one of possible reason is supplied with largely
Nitrogen can promote Photosynthesis in Soybean product to root system distribution, reduce its transhipment into root nodule, reduce nodule number, influence root nodule class bacterium
Body develops and then reduces nitrogenase activity (Fujikake et al., 2003).However, not supplying nitrogen completely cannot also expire
Needed for sufficient soybean growth, the demand of growth and development especially before the effective fixed nitrogen of root nodule.Therefore, by soybean biological nitrogen fixation
It must be combined with rational nitrogen management measure applied to production practices.Soybean hydroponic system contains convenient for control supply nutrient
Amount, is one of important culture means of Plant Nutrition research.So making suitable for soybean nodulation and yield can be improved
Mill water culture nutrient solution has important finger to winter greenhouse/growth room's breeding and to the development weight-reducing sustainable ecological agriculture of synergy
It leads and practice significance.
Invention content
Based on the studies above background, problem to be solved by this invention be to provide a kind of combination Rhizobium Inoculation technology,
It is suitble to soybean nodulation, plant strain growth healthy and strong simultaneously, in medium nitrogen level（2.4mM）Under significantly improve the water planting nutrition of soybean yields
Liquid.
In order to solve the above technical problems, the present invention provides a kind of mill water culture nutrient solution improving soybean yields and its preparation and makes
With method, nutrient composition includes mainly：0.68 mM of potassium nitrate, 0.54 mM of calcium nitrate, 0.66 mM of calcium chloride, ammonium nitrate
0.18 mM, 0.71 mM of potassium sulfate, 0.5 mM of magnesium sulfate, 0.025 mM of magnesium chloride, 0.0015 mM of manganese sulfate, ammonium sulfate 0.14
MM, 0.0015 mM of zinc sulfate, 0.0005 mM of copper sulphate, 0.00015 mM of ammonium molybdate, 0.0025 mM of Boratex, cobalt chloride
0.5 mM of 0.0001 mM, EDTA- molysite, 0.04 mM and potassium dihydrogen phosphate.The nutrient solution is by following 8 mother liquors (stock)
It configures, wherein stock1 includes potassium nitrate, calcium nitrate and ammonium nitrate；Stock2 includes ammonium sulfate；Stock3 includes sulfuric acid
Magnesium, magnesium chloride, manganese sulfate, zinc sulfate, copper sulphate, ammonium molybdate and cobalt chloride；Stock4 includes Boratex；Stock5 includes
EDTA- molysite；Stock6 includes potassium dihydrogen phosphate；Stock7 includes potassium sulfate；Stock8 includes calcium chloride.
A kind of preparation method of mill water culture nutrient solution improving soybean yields as described above, specifically includes：By stock1-
Stock8 dilutes 1000 times, takes stock1-stock8 to dilute the liquid mixing after 1000 times, obtains nutrient solution.
When using Solution culture method soybean, key method therein is Rhizobium Inoculation measure to be coordinated, and is specifically included：Match
The nutrient solution set, with 0.5 mM H2SO4Or 1 mM KOH pH is adjusted to 5.7-5.9 after, packing is to incubator；It will grow 7 days
Soybean seedling, be put into after being impregnated 1 hour in the root nodule bacteria liquid of OD ≈ 1, and migrate in the nutrient solution after adjusting pH, often
10 min of hour ventilation, pH of Additional nutrient solution and tune, makes pH maintain 5.7-5.9 every three days.
The invention has the beneficial effects that：Use soybean mill water culture nutrient solution provided by the invention（Nitrogen concentration total amount is
2.4mM）Coordinate Rhizobium Inoculation technology, soybean nodulation can be obviously promoted, increases nodule number and weight, can finally significantly improve
Soybean yields.It is more more efficient than lift-augmented mechanism, it is a kind of low input high production, environmentally friendly plantation（Breeding）Pattern.This
Outside, soybean greenhouse/growth room's completion breeding work in winter can be made using this water culture technique, while development weight-reducing synergy can be held
The continuous ecological agriculture has important guidance and practice significance.
Description of the drawings
Fig. 1 is influence of the Different nitrogen levels to soybean nodulation and growth；
Fig. 2 bears pods to soybean for Rhizobium Inoculation under the conditions of Different nitrogen levels and the influence of yield.
Specific implementation mode
Specific introduce is made to the present invention below in conjunction with the drawings and specific embodiments.
Embodiment 1：Configuration suitable for soybean nodulation mill water culture nutrient solution
A kind of mill water culture nutrient solution and method improving soybean yields provided by the invention, key component include：Potassium nitrate 0.68
MM, 0.54 mM of calcium nitrate, 0.66 mM of calcium chloride, 0.18 mM of ammonium nitrate, 0.71 mM of potassium sulfate, 0.5 mM of magnesium sulfate, chlorination
0.025 mM of magnesium, 0.0015 mM of manganese sulfate, 0.14 mM of ammonium sulfate, 0.0015 mM of zinc sulfate, 0.0005 mM of copper sulphate, molybdic acid
0.00015 mM of ammonium, 0.0025 mM of Boratex, 0.0001 mM, EDTA- molysite of cobalt chloride, 0.04 mM and potassium dihydrogen phosphate 0.5
mM.The nutrient solution is configured by following 8 mother liquors (stock), wherein stock1 includes potassium nitrate, calcium nitrate and nitre
Sour ammonium；Stock2 includes ammonium sulfate；Stock3 includes magnesium sulfate, magnesium chloride, manganese sulfate, zinc sulfate, copper sulphate, ammonium molybdate and chlorine
Change cobalt；Stock4 includes Boratex；Stock5 includes EDTA- molysite；Stock6 includes potassium dihydrogen phosphate；Stock7 includes sulfuric acid
Potassium；Stock8 includes calcium chloride.
Mentioned reagent is weighed respectively, is dissolved in distilled water, blue lid bottle is stored in after constant volume, prepares stock liquid, stock liquid
In the concentration of each ingredient be final concentration（Nutrient solution）1000 times.Wherein, stock5 molysite needs to be stored in brown bottle.It takes
The liquid that stock1- stock8 dilute after 1000 times mixes in equal volume, obtains nutrient solution.
Embodiment 2：Influence of the Different nitrogen levels to soybean nodulation and growth
Germination 7 days in quartz sand soya seeds HN66, the rhizobium of inoculation OD ≈ 1（BXYD3）It is shifted respectively after 1 hour
Include to different N concentration：0.3, the nutrient solution of 0.9,2.4,4.8., 7.2 mM（pH=5.7-5.9）Middle processing.It ventilates per hour
10 min adjust a pH, pH are made to maintain 5.7-5.9 every three days.Soybean grows to the R1 phases and observes overground part phenotype, Radical extension
Situation simultaneously further counts nodule number and nodule weight.
Fig. 1 is influence of the Different nitrogen levels to soybean nodulation and growth.Wherein：
In Fig. 1（A）For soybean overground part phenotype；
In Fig. 1（B）It is crosscutting for soybean nodulation；
In Fig. 1（C）For nodule number；
In Fig. 1（D）For root nodule fresh weight.
Data are the average value and standard error that 4 biology repeat in figure.
The result shows that：
Rhizobium Inoculation can remarkably promote soybean growth, and overground part is luxuriant with the increase of nitrogen concentration, referring to Fig. 1（A）.So
And the R1 phases are grown in soybean, find too low nitrogen（0.3 mM）With high nitrogen（4.8 and 7.2 mM）Processing significantly inhibits soybean knot
Tumor, during nodule number and weight on taproot and lateral root are considerably less than（2.4 mM）It is low（0.9 mM）Nitrogen level processing, referring to
In Fig. 1（B, C and D）.Illustrate, Rhizobium Inoculation can promote soybean nodulation and soybean to grow, fully send out in, under low nitrogen level
Soybean biological nitrogen fixation is waved, extraneous rational nitrogen concentration is needed.
Embodiment 3：Rhizobium Inoculation bears pods to soybean and the influence of yield under the conditions of Different nitrogen levels
After the soybean seedling Rhizobium Inoculation USDA110 of growth 7 days, moves to respectively in the nutrient solution of different N concentration and grow to knot
Pod harvest time, soybean pod number and yield effect under comparative analysis Different nitrogen levels.
Fig. 2 bears pods to soybean for Rhizobium Inoculation under the conditions of Different nitrogen levels and the influence of yield.Wherein：
In Fig. 2（A）For the pod of same section under Different nitrogen levels；
In Fig. 2（B）For soybean per plant seed；
In Fig. 2（C）For beanpod weight；
In Fig. 2（D）For 100-grain weight.
Data are the average value and standard error that 4 biology repeat in figure.
Asterisk indicates Rhizobium Inoculation and the not significant difference under the conditions of Rhizobium Inoculation between same nitrogen level processing：
* the level of signifiance 0.01 is indicated<When ρ≤0.05, significant difference；
* indicates the level of signifiance 0.001<When ρ≤0.01, the significance of difference between significantly with extremely significantly between；
Ns indicates no significant difference.
The result shows that：
Except 0.3 mM of low nitrogen（Beanpod quantity is few, referring to A in Fig. 2）Outer with the 7.2 mM processing of high nitrogen, Rhizobium Inoculation can be significantly
Increase soybean pod number, referring to C in Fig. 2, in, under low nitrogen level, Rhizobium Inoculation can increase soybean yields.In addition, nitrogen level
After reaching 2.4 mM, soybean yields not with nitrogen supply increase and increase, with increasing for nitrogen, have instead subtract it is low-producing
Trend, referring to B in Fig. 2 and D.Therefore, 2.4 mM nitrogen combination Rhizobium Inoculation technologies of extraneous supply, can give full play to soybean biological
Nitrogen fixation improves soybean yields under the conditions of water planting, is nitrogen concentration more suitable in hydroponic system.
In conclusion the present invention provides a kind of mill water culture nutrient solution and method improving soybean yields, in conjunction with inoculation root nodule
Bacterium technology can give full play to biological nitrogen fixation, in medium nitrogen level（2.4 mM）Under the conditions of, soybean production can be significantly improved
Amount, it is more more efficient than lift-augmented mechanism, it is a kind of low input high production, environmentally friendly plantation（Breeding）Pattern.Utilize this water
Training system can carry out soybean breeding in greenhouse/growth room, take up space small, the features such as being easy to control.The water that inventor provides
Cultivation nutrient fluid and method can provide theoretical foundation, to development weight-reducing synergy to improve the nitrogen nutrition management mode of soybean yields
The sustainable ecological agriculture has important guidance and practice significance.
It should be noted that the invention is not limited in any way for above-described embodiment, it is all to use equivalent replacement or equivalent change
The technical solution that the mode changed is obtained, all falls in protection scope of the present invention.
Claims (4)
1. a kind of mill water culture nutrient solution improving soybean yields, which is characterized in that nutrient composition includes following components：Potassium nitrate
0.68 mM, 0.54 mM of calcium nitrate, 0.66 mM of calcium chloride, 0.18 mM of ammonium nitrate, 0.71 mM of potassium sulfate, 0.5 mM of magnesium sulfate,
0.025 mM of magnesium chloride, 0.0015 mM of manganese sulfate, 0.14 mM of ammonium sulfate, 0.0015 mM of zinc sulfate, 0.0005 mM of copper sulphate,
0.00015 mM of ammonium molybdate, 0.0025 mM of Boratex, 0.0001 mM, EDTA- molysite of cobalt chloride, 0.04 mM and potassium dihydrogen phosphate
0.5 mM。
2. a kind of mill water culture nutrient solution improving soybean yields according to claim 1, it is characterised in that：The nutrient solution by
8 stock are configured below, wherein stock1 includes potassium nitrate, calcium nitrate and ammonium nitrate；Stock2 includes ammonium sulfate；
Stock3 includes magnesium sulfate, magnesium chloride, manganese sulfate, zinc sulfate, copper sulphate, ammonium molybdate and cobalt chloride；Stock4 includes Boratex；
Stock5 includes EDTA- molysite；Stock6 includes potassium dihydrogen phosphate；Stock7 includes potassium sulfate；Stock8 includes calcium chloride.
3. improving the preparation method of the mill water culture nutrient solution of soybean yields as claimed in claim 2, which is characterized in that the system
Preparation Method includes：Stock1-stock8 is diluted 1000 times, takes stock1-stock8 to dilute the liquid mixing after 1000 times, obtains
To nutrient solution.
4. improving the application method of the mill water culture nutrient solution of soybean yields as claimed in claim 2, which is characterized in that use battalion
When nutrient solution culture soybean, include the following steps：
A, the nutrient solution configured, with 0.5 mM H2SO4Or 1 mM KOH pH is adjusted to 5.7-5.9 after, packing is to incubator；
B, will 7 days soybean seedling Rhizobium Inoculations of growth, and migrate in the nutrient solution after adjusting pH, ventilate 10 per hour
Min, pH of Additional nutrient solution and tune, makes pH maintain 5.7-5.9 every three days.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102060611A (en) * | 2010-11-26 | 2011-05-18 | 合肥工业大学 | Liquid composite foliar fertilizer special for soybean |
| CN102584384A (en) * | 2011-12-29 | 2012-07-18 | 东北农业大学 | Phosphorus concentration nutrient solution for soybean sand culture |
| CN102701861A (en) * | 2012-06-21 | 2012-10-03 | 魏玉芳 | Plant nutrient liquid fertilizer |
| CN103952341A (en) * | 2014-04-16 | 2014-07-30 | 四川农业大学 | Azorhizobium strain SCAUs152 and application thereof |
| CN107500864A (en) * | 2017-08-28 | 2017-12-22 | 珠海市绿之宝农业有限公司 | A kind of vegetable aquaculture nutrient solution and preparation method thereof and application method |
-
2018
- 2018-06-05 CN CN201810570710.6A patent/CN108558518A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102060611A (en) * | 2010-11-26 | 2011-05-18 | 合肥工业大学 | Liquid composite foliar fertilizer special for soybean |
| CN102584384A (en) * | 2011-12-29 | 2012-07-18 | 东北农业大学 | Phosphorus concentration nutrient solution for soybean sand culture |
| CN102701861A (en) * | 2012-06-21 | 2012-10-03 | 魏玉芳 | Plant nutrient liquid fertilizer |
| CN103952341A (en) * | 2014-04-16 | 2014-07-30 | 四川农业大学 | Azorhizobium strain SCAUs152 and application thereof |
| CN107500864A (en) * | 2017-08-28 | 2017-12-22 | 珠海市绿之宝农业有限公司 | A kind of vegetable aquaculture nutrient solution and preparation method thereof and application method |
Non-Patent Citations (1)
| Title |
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| 李凯等: "铵硝配比对大豆生长及结瘤固氮的影响", 《中国油料作物学报》 * |
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Application publication date: 20180921 |