CN108543117A - A kind of barrier holder and preparation method thereof - Google Patents
A kind of barrier holder and preparation method thereof Download PDFInfo
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- CN108543117A CN108543117A CN201810448834.7A CN201810448834A CN108543117A CN 108543117 A CN108543117 A CN 108543117A CN 201810448834 A CN201810448834 A CN 201810448834A CN 108543117 A CN108543117 A CN 108543117A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/042—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/02—Inorganic materials
- A61L31/022—Metals or alloys
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/02—Inorganic materials
- A61L31/024—Carbon; Graphite
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/02—Inorganic materials
- A61L31/026—Ceramic or ceramic-like structures, e.g. glasses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/02—Inorganic materials
- A61L31/028—Other inorganic materials not covered by A61L31/022 - A61L31/026
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
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Abstract
The present invention provides a kind of preparation methods of barrier holder, include the following steps：A) microorganism fungus kind is seeded in solid substrate, surface is obtained after fermentation has the solid substrate of bacteria cellulose film；The microorganism fungus kind is the strain that can generate bacteria cellulose；B) by step A) obtained solid substrate is positioned in the aerosol containing nutrient solution, ferments；C metallic support) is placed in step B) obtained by solid substrate surface, obtain sectional shelf-unit；D) sectional shelf-unit is positioned in the aerosol containing nutrient solution, is fermented, barrier holder is obtained.The preparation method of barrier holder provided by the present application can be prepared the clinical timbering material with good barrier function, and barrier cell, bacterium while can penetrate growth factor again, and have good biocompatibility, be a kind of ideal clinical to obstruct holder.
Description
Technical field
The present invention relates to technical field of composite materials more particularly to a kind of barrier holder and preparation method thereof.
Background technology
Bone tissue is a few one of histoorgan with self-regeneration ability, this power of regeneration not only along with
The skeleton development and bone tissue injury repair process of individual, or even still can effectively work after a body maturation.
Guided tissue regeneration (Guided Tissue Regeneration, GTR) is to place one using the method for surgery
A physical barriers selectively separate different tissues.By vicennial exploration, guided bone tissue is had been established at present
Regeneration, and therefrom amplify out the basic conception of guided bone regeneration, that is, utilize barrier film shielding action by surrounding soft tissue and wound
Area bone face separates, and such as tooth is prevented to grow into form fibrous connective tissue with fibroblast, osteoblast at bone face is enable to have enough
Time proliferation, reconstruction of bone tissue.The successful key of Guided Bone Regeneration technology is that blocking soft tissue grows into bone defect area, this will
Ask the pore structure of membrane material rationally or membrane material tight, to achieve the purpose that not allow soft tissue cells by wanting simultaneously
Membrane material is asked to act on the growth unrestraint of osteoblast.
Currently, membrane material include mainly with nonabsorable film that polytetrafluoroethylene (PTFE) (PTFE) is representative and with polylactic acid-based or
Collagen class material is 2 major class of Absorbable membrane prepared by raw material.There is finished product sale in this 2 class film foreign countries, but price is all extremely high
It is expensive, it is difficult to adapt to the needs of domestic plantation patient.We study the domestic membrane material that can make GTR applications thus, to domestic e-
PTFE film and homemade chitnous membrane carry out the basic research of membrane material, inquire into it and are used for the possibility of GTR.
E-PTFE films are polyflon manufactured medical macromolecular materials, biofacies after heat stretching process is handled
Capacitive is good, and physicochemical property is stablized, and disinfection is easy to use.The product is widely used in fracture, rupture of tendon, blood vessel and internal organs and wears
The repairing in hole.In dentistry field, e-PTFE is mainly used for Guided Bone Regeneration when periodontal disease therapeutic and Dental implantion；For tooth kind
The e-PTFE films of the finished product of plant have Gore_Tex and Im-pra etc., due to production technology and equipment category patent protection, product valence
Lattice are sufficiently expensive.Therefore, a kind of cheap, timbering material of function admirable is developed, it is particularly significant to clinical application.
Invention content
Present invention solves the technical problem that being to provide a kind of preparation method of barrier holder, barrier branch prepared by the application
Frame is a kind of clinical timbering material with good barrier function.
In view of this, this application provides a kind of preparation method of barrier holder, include the following steps：
A) microorganism fungus kind is seeded in solid substrate, surface is obtained after fermentation has the solid-based of bacteria cellulose film
Bottom；The microorganism fungus kind is the strain that can generate bacteria cellulose；
B) by step A) obtained solid substrate is positioned in the aerosol containing nutrient solution, ferments；
C metallic support) is placed in step B) obtained by solid substrate surface, obtain sectional shelf-unit；
D) sectional shelf-unit is positioned in the aerosol containing nutrient solution, is fermented, barrier holder is obtained.
Preferably, in the aerosol further include one or both of function nano particle and pore creating material.
Preferably, the function nano particle is selected from carbon nanotube, graphene oxide, clay piece, boron nitride nanosheet, two
It is one or more in molybdenum sulfide nanometer sheet, ferroferric oxide nano granules, zinc oxide nanowire and gold nano grain；It is described to make
Hole agent is one or more in porous silicon, zeolite granular, calcium carbonate granule, hydroxyapatite and cellular glass.
Preferably, the solid substrate is selected from acetic acid bacteria surface culture medium, bacteria cellulose film, non-woven fabrics, plastics
One kind in plate, glass, plank and metal；The nutrient solution be by the glucose containing 5wt%~10wt% and 1wt%~
The aqueous solution of the yeast powder of 5wt% obtains after sterilizing.
Preferably, the solid substrate is acetic acid bacteria surface culture medium, and the acetic acid bacteria surface culture medium is
By the glucose containing 10wt%, the yeast powder of 1wt%, the calcium carbonate of 2wt% and 1.5wt% agar aqueous solution through boiling
Boiling, high-pressure sterilizing pot sterilize and stand cooling obtained plating medium.
Preferably, step A) in, the temperature of the fermentation is 15~33 DEG C, and the time is 24~48h.
Preferably, step B) in, the temperature of the fermentation is 15~33 DEG C, and the time is 1~5 day.
Preferably, step D) further include later：
Barrier holder is taken out from solid substrate, is impregnated in lye again after being rinsed using clear water, then uses clear water anti-
It is multiple to rinse.
Present invention also provides a kind of barrier holders, including metallic support and the bacterium for being coated on the metal support surface
Cellulose membrane, the bacteria cellulose film are obtained by microorganism fungus kind through everfermentation, and the microorganism fungus kind is that can generate bacterium
The strain of cellulose.
Preferably, the aperture of the bacteria cellulose film is less than 100nm.
This application provides a kind of preparation methods of barrier holder, and microorganism fungus kind is seeded to solid substrate first
On, make microorganism fungus kind fermentation to form bacteria cellulose film in solid substrate surface, then obtained solid substrate is positioned over
It ferments in aerosol containing nutrient solution, obtains that there is certain thickness bacteria cellulose film, be then positioned over metallic support
Obtained solid substrate surface makes metallic support be contacted with bacteria cellulose film and ferments again, metallic support is made to be embedded in bacterium
Inside cellulose, the bacteria cellulose film with the filling of certain thickness metallic support is formed.The barrier that the application is prepared
Holder is the structure that bacteria cellulose film coats metallic support, and the effect of metallic support is plasticity and support, bacteria cellulose film
It is the fine and close network structure formed by nano-cellulose, which can effectively obstruct cell and bacterium passes through；Further, this Shen
Please by the way that pore creating material is added, the aperture of bacteria cellulose film is increased, is conducive to the small sizes factor such as growth factor and passes through, simultaneously
Can histocyte, bacterium pass through.Therefore, the barrier holder that the application is prepared has preferable barriering effect.
Description of the drawings
Fig. 1 is the pattern photo of barrier holder prepared by embodiment 1；
Fig. 2 be embodiment 1 prepare barrier holder can fix, the photo of plastic type；
Fig. 3 is that the ti-alloy mesh that embodiment 2 is prepared fills the compound barrier holder of bacteria cellulose/hydroxyapatite
Digital photograph；
Fig. 4 is that the ti-alloy mesh that embodiment 2 is prepared fills the compound barrier holder of bacteria cellulose/hydroxyapatite
Electron scanning micrograph (amplification factor 8.28Kx)；
Fig. 5 is that the ti-alloy mesh that embodiment 2 is prepared fills the compound barrier holder of bacteria cellulose/hydroxyapatite
Electron scanning micrograph (amplification factor 16.62Kx)；
Fig. 6 is that the ti-alloy mesh that embodiment 2 is prepared fills the compound barrier holder of bacteria cellulose/hydroxyapatite
Electron scanning micrograph (amplification factor 5.93Kx)；
Fig. 7 is the number that the ti-alloy mesh that embodiment 3 is prepared fills the compound barrier holder of bacteria cellulose/carbon nanotube
Code photo；
Fig. 8 is that the ti-alloy mesh that embodiment 3 is prepared fills the compound barrier holder difference of bacteria cellulose/carbon nanotube
The electron scanning micrograph (10.00Kx) of amplification factor；
Fig. 9 is that the ti-alloy mesh that embodiment 3 is prepared fills the compound barrier holder difference of bacteria cellulose/carbon nanotube
The electron scanning micrograph (30.00Kx) of amplification factor.
Specific implementation mode
For a further understanding of the present invention, the preferred embodiment of the invention is described with reference to embodiment, still
It should be appreciated that these descriptions are only the feature and advantage further illustrated the present invention, rather than to the claims in the present invention
Limitation.
The characteristics of for holder is obstructed in the prior art, this application provides a kind of preparation method of barrier holder, the resistances
Every holder be substantially it is a kind of bacteria cellulose film generated based on bacterial fermentation and fill the barrier holder of metallic support, the barrier branch
Frame has good barriers function, can penetrate growth factor while can obstructing bacterium, cell again, and have good biofacies
Capacitive is a kind of ideal clinical barrier holder.Specifically, the preparation method that the present invention obstructs holder includes the following steps：
A) microorganism fungus kind is seeded in solid substrate, surface is obtained after fermentation has the solid-based of bacteria cellulose film
Bottom；The microorganism fungus kind is the strain that can generate bacteria cellulose；
B) by step A) obtained solid substrate is positioned in the aerosol containing nutrient solution, ferments；
C metallic support) is placed in step B) obtained by solid substrate surface, obtain sectional shelf-unit；
D) sectional shelf-unit is positioned in the aerosol containing nutrient solution, is fermented, barrier holder is obtained.
During the application prepares barrier holder, the preparation of raw material is carried out first, that is, prepares microorganism fungus kind, solid
Substrate and preparation nutrient solution；Above-mentioned raw materials are commercial product, and the application is not particularly limited its source.In the application
In, the microorganism fungus kind is the strain that can generate bacteria cellulose, and the present invention does not limit the microorganism fungus kind particularly
System, as long as bacteria cellulose can be generated；Specifically, its can be selected from acetic acid Pseudomonas, Agrobacterium rhizobium and
It is one or more in Sarcina；In this application, the microorganism fungus kind is selected from gluconate pyracetobacillus or the Portugals Han Shi
Sweet and sour bacillus.
The application is not particularly limited the solid substrate, as long as that can support the solid material of growth i.e.
It can；Specifically, the solid substrate is selected from acetic acid bacteria surface culture medium, bacteria cellulose film, non-woven fabrics, plastic plate, glass
One kind in glass, plank and metal；In a particular embodiment, the solid substrate is selected from acetic acid bacteria surface culture medium,
Be by the glucose containing 10wt%, the yeast powder of 1wt%, the calcium carbonate of 2wt%, 1.5wt% agar aqueous solution pass through
It boils, high pressure sterilization and stand cooling obtained substrate；When herein described solid substrate selects other substrates, being can not be straight
The substrate for connecing offer bacteriotrophy needs to reuse after soaking using nutrient solution after sterilizing.
According to the present invention, then microorganism fungus kind is seeded in solid substrate after raw material preparation, is obtained after fermentation
Surface has the solid substrate of bacteria cellulose film；In the process, microorganism fungus kind fermentation is formed carefully in solid substrate surface
Fungin film.In above process, microorganism fungus kind is preferably seeded to solid by the pollution of other bacteriums in order to prevent, the application
In body substrate, place into insulating box.The temperature of the insulating box is 15~33 DEG C, in a particular embodiment, the insulating box
Temperature be 28~30 DEG C；The time of the culture is 24~48h, and in a particular embodiment, the time of the culture is 36h.
After the culture of insulating box, a complete bacteria cellulose film is formd in solid substrate surface.
Then surface obtained above there is the solid substrate of bacteria cellulose film to be positioned over containing nutrient solution by the application
Aerosol in, fermentation, with solid substrate surface formed have certain thickness bacteria cellulose film.In above process,
The nutrient solution provides nutrition for the fermentation growth of microorganism fungus kind, is sent out for bacteria cellulose well known to those skilled in the art
Ferment growth need nutrient solution, this application is not particularly limited, in a particular embodiment, the nutrient solution be containing
The aqueous solution of the glucose of 5wt%~10wt% and the yeast powder of 1wt%~5wt%, needs sterilize in high-pressure sterilizing pot
It uses again afterwards.Further include in function nano particle and pore creating material in the aerosol to improve the functionality of barrier holder
It is one or two kinds of；Specifically, the function nano particle is function nano particle solution or function nano particle dry powder；The work(
Energy nano particle source by high pressure sterilization or is dispersed in sterile water or is made sterile dry by function nano particle aqueous solution；Tool
Body, the function nano particle is selected from carbon nanotube, graphene oxide, clay piece, boron nitride nanosheet, molybdenum disulfide nano
It is one or more in piece, ferroferric oxide nano granules, zinc oxide nanowire and gold nano grain；The pore creating material is itself
With pore passage structure or bacteria cellulose aquagel can be caused to form the material of pore passage structure, be selected from porous silica, zeolite
One or more of particle, hydroxyapatite, cellular glass and calcium carbonate granule.The temperature of the fermentation is 15~33
DEG C, in a particular embodiment, the temperature of the fermentation is 28~30 DEG C；The time of the fermentation is 1~5 day, is being embodied
In example, the time of the fermentation is 2~5 days.In above-mentioned fermentation process, due to containing function nano particle and/or pore creating material,
The hole formed containing function nano particle and pore creating material in the bacteria cellulose film then formed.
According to the present invention, then place metallic support on the surface for forming bacteria cellulose film, and make metallic support with it is thin
Fungin film fits closely.By metallic support branch is referred to as combined with certain thickness bacteria cellulose film with above-mentioned formation
Frame.Herein described metallic support is metallic support well known to those skilled in the art, is not particularly limited to this application,
Exemplary, the metallic support is the different metal of different shapes of plasticity, and specific shape can be metal wire, metal
Net, sheet metal and pattern metal etc.；In a particular embodiment, the metallic support is titanium net.
Said combination holder is finally positioned in the aerosol containing nutrient solution by the application, fermentation, after cultivating several days,
Metallic support is embedded in the inside of bacteria cellulose film, and form metallic support filling has certain thickness bacterial fibers
Plain film.In the case of containing function nano particle and/or pore creating material in aerosol, the tool of metallic support filling has been finally obtained
There is certain thickness bacteria cellulose/function nano Particles dispersed film.The bacterium for the metallic support filling that the application is prepared
Cellulose/function nano Particles dispersed film shows different colors due to the difference of function nano particle, such as：Bacterium is fine
Dimension element/carbon nano-tube compound film is black, and bacteria cellulose/ferroso-ferric oxide composite membrane is in sepia, bacteria cellulose/clay
Piece composite membrane is white etc..
Above-mentioned fermentation process with formed have the fermentation process of certain thickness bacteria cellulose film it is identical, herein without
It repeats.Contain plurality of impurities in the barrier rack surface of formation, therefore the application preferably removes barrier holder from solid substrate,
It impregnates in lye after being rinsed using clear water, is then rinsed to neutrality using clear water.The lye is specially a concentration of 1wt%
The time of the sodium hydroxide solution of~3wt%, the immersion is 6~36h；In a particular embodiment, the sodium hydroxide is water-soluble
The time of a concentration of 2wt% of liquid, the immersion are for 24 hours.
Present invention also provides a kind of barrier holders comprising metallic support be coated on the thin of the metal support surface
Fungin film, the bacteria cellulose film are obtained by microorganism fungus kind through everfermentation, and the microorganism fungus kind is that can generate carefully
The strain of fungin.
Further include nano functional in the bacteria cellulose film to provide the functionality of barrier holder according to the present invention
Particle, the functional nanoparticles are chosen in particular from carbon nanotube, graphene oxide, clay piece, boron nitride nanosheet, molybdenum disulfide
It is one or more in nanometer sheet, ferroferric oxide nano granules, zinc oxide nanowire and gold nano grain.It is herein described thin
The aperture of fungin film is less than 100nm.
This application provides a kind of preparation methods of barrier holder, and the clinic with good barrier function can be prepared
Timbering material can penetrate growth factor while obstructing cell, bacterium, and have good biocompatibility again, be a kind of
Ideal clinical barrier holder.It is provided by the invention barrier holder preparation method it is simple, it is at low cost, environmentally protective, can scale
Metaplasia is produced, and has effectively been expanded the application field of bacteria cellulose, has been with a wide range of applications.
For a further understanding of the present invention, with reference to embodiment to barrier holder provided by the invention and preparation method thereof
It is described in detail, protection scope of the present invention is not limited by the following examples.
Embodiment 1
The aqueous solution of 500mL glucose containing 10wt%, 1wt% yeast powders, 2wt% calcium carbonate, 1.5wt% agar is prepared,
Stirring and dissolving is simultaneously boiled, and in packing to the beaker of 5 250mL, is sealed beaker with tissue culture film, high-pressure sterilizing pot sterilizing, cooling
Standing obtains solid substrate；The aqueous solution for containing 5wt% glucose and 1wt% yeast powders is prepared as nutrient solution；Prepare metal
Titanium net simultaneously sterilizes spare；
Gluconate pyracetobacillus strain is seeded in above-mentioned solid substrate, constant incubator is put into, is cultivated at 28 DEG C
24h；
Solid substrate obtained above is transferred to by constant incubator in the aerosol spray that nutrient solution is formed, nutrient solution
Spraying speed is adjusted to 2mL/ hours；
After the above process ferments 2 days, the titanium net after being formed by bacteria cellulose composite membrane surface and placing sterilizing repeats
Above-mentioned fermentation process 3 days；
Gained holder is taken out from solid culture primary surface, the hydroxide of mass fraction 2% is put into after being rinsed using clear water
It is impregnated in sodium water solution for 24 hours, taking-up is impregnated with clear water, rinsed to neutrality, and barrier holder is obtained.
Fig. 1 is the digital photograph that the ti-alloy mesh that the present embodiment is prepared fills the compound barrier holder of bacteria cellulose；
Fig. 2 is that the ti-alloy mesh that the present embodiment is prepared is filled the compound barrier holder plastic type of bacteria cellulose, can be consolidated
Fixed photo.
Embodiment 2
The aqueous solution of 500mL glucose containing 10wt%, 1wt% yeast powders, 2wt% calcium carbonate, 1.5wt% agar is prepared,
Stirring and dissolving is simultaneously boiled, and high-pressure sterilizing pot sterilizing is poured into the square tray of length of side 40cm, cooling and standings obtain solid-based
Bottom；The aqueous solution containing 5wt% glucose Yu 1wt% yeast powders is prepared, as nutrient solution；Prepare 3mg/mL's using sterile water
Nanometer hydroxyapatite solution；Prepare ti-alloy mesh and sterilizes spare；
Gluconate pyracetobacillus strain is seeded in above-mentioned solid substrate, constant incubator is put into, is cultivated at 28 DEG C
24h；
Solid substrate obtained above is transferred to nutrient solution by constant incubator, nanometer hydroxyapatite solution is formed
Aerosol spray in, two kinds of solution spraying speed is all adjusted to 2mL/ hours；
After the above process ferments 3 days, the titanium net after being formed by bacteria cellulose composite membrane surface and placing sterilizing repeats
Above-mentioned fermentation process 4 days；
Holder obtained above is taken out from solid substrate surface, the hydrogen of mass fraction 2% is put into after being rinsed using clear water
It is impregnated in aqueous solution of sodium oxide for 24 hours, taking-up is impregnated with clear water, rinsed to neutrality, and barrier holder is obtained.
Fig. 3 is that the ti-alloy mesh that the present embodiment is prepared fills the compound barrier holder of bacteria cellulose/hydroxyapatite
Digital photograph；
Fig. 4,5,6 are that the ti-alloy mesh that the present embodiment is prepared fills the compound barrier of bacteria cellulose/hydroxyapatite
Electron scanning micrograph under holder different amplification.
Embodiment 3
The aqueous solution of 500mL glucose containing 10wt%, 1wt% yeast powders, 2wt% calcium carbonate, 1.5wt% agar is prepared,
Stirring and dissolving is simultaneously boiled, and in packing to the beaker of 5 250mL, is sealed beaker with tissue culture film, high-pressure sterilizing pot sterilizing, cooling
Standing obtains solid substrate；The aqueous solution containing 5wt% glucose Yu 1wt% yeast powders is prepared, as nutrient solution；Using sterile
Water prepares the carbon nanotube of 1mg/mL；Prepare ti-alloy mesh and sterilizes spare；
Gluconate pyracetobacillus strain is seeded in above-mentioned solid substrate, constant incubator is put into, is cultivated at 28 DEG C
24h；
Solid substrate obtained above is transferred to nutrient solution by constant incubator, carbon nanotube is formed by aerosol spray
In mist, two kinds of solution spraying speed are all adjusted to 2mL/ hours；
After the above process ferments 2 days, the titanium net after being formed by bacteria cellulose composite membrane surface and placing sterilizing repeats
Above-mentioned fermentation process 3 days；
Gained holder is taken out from solid culture primary surface, the hydroxide of mass fraction 2% is put into after being rinsed using clear water
It is impregnated in sodium water solution for 24 hours, taking-up is impregnated with clear water, rinsed to neutrality, and barrier holder is obtained.
Fig. 7 is that the ti-alloy mesh that the present embodiment is prepared fills the compound barrier holder of bacteria cellulose/carbon nanotube
Digital photograph；
The compound barrier holder of bacteria cellulose/carbon nanotube is filled in Fig. 8,9 for the ti-alloy mesh that the present embodiment is prepared
The electron scanning micrograph of different amplification.
The explanation of above example is only intended to facilitate the understanding of the method and its core concept of the invention.It should be pointed out that pair
For those skilled in the art, without departing from the principle of the present invention, the present invention can also be carried out
Some improvements and modifications, these improvement and modification are also fallen within the protection scope of the claims of the present invention.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest range caused.
Claims (10)
1. a kind of preparation method of barrier holder, includes the following steps：
A) microorganism fungus kind is seeded in solid substrate, surface is obtained after fermentation has the solid substrate of bacteria cellulose film；
The microorganism fungus kind is the strain that can generate bacteria cellulose；
B) by step A) obtained solid substrate is positioned in the aerosol containing nutrient solution, ferments；
C metallic support) is placed in step B) obtained by solid substrate surface, obtain sectional shelf-unit；
D) sectional shelf-unit is positioned in the aerosol containing nutrient solution, is fermented, barrier holder is obtained.
2. preparation method according to claim 1, which is characterized in that further include in the aerosol function nano particle and
One or both of pore creating material.
3. preparation method according to claim 2, which is characterized in that the function nano particle is selected from carbon nanotube, oxygen
Graphite alkene, clay piece, boron nitride nanosheet, molybdenum disulfide nano sheet, ferroferric oxide nano granules, zinc oxide nanowire and
It is one or more in gold nano grain；The pore creating material is selected from porous silicon, zeolite granular, calcium carbonate granule, hydroxyapatite
With it is one or more in cellular glass.
4. preparation method according to claim 1 or 2, which is characterized in that the solid substrate is flat selected from acetic acid bacteria solid
One kind in face culture medium, bacteria cellulose film, non-woven fabrics, plastic plate, glass, plank and metal；The nutrient solution is by containing
There are the glucose of 5wt%~10wt% and the aqueous solution of the yeast powder of 1wt%~5wt% to be obtained after sterilizing.
5. preparation method according to claim 4, which is characterized in that the solid substrate is acetic acid bacteria surface culture
Base, the acetic acid bacteria surface culture medium are the calcium carbonate by the glucose containing 10wt%, the yeast powder of 1wt%, 2wt%
With the aqueous solution of the agar of 1.5wt% through boiling, high-pressure sterilizing pot sterilizes and stands cooling obtained plating medium.
6. preparation method according to claim 1 or 2, which is characterized in that step A) in, the temperature of the fermentation is 15~
33 DEG C, the time is 24~48h.
7. preparation method according to claim 1 or 2, which is characterized in that step B) in, the temperature of the fermentation is 15~
33 DEG C, the time is 1~5 day.
8. preparation method according to claim 1 or 2, which is characterized in that step D) further include later：
Barrier holder is taken out from solid substrate, impregnates in lye after being rinsed using clear water, is then rushed repeatedly using clear water again
It washes.
9. a kind of barrier holder, including metallic support and the bacteria cellulose film for being coated on the metal support surface, described thin
Fungin film is obtained by microorganism fungus kind through everfermentation, and the microorganism fungus kind is the strain that can generate bacteria cellulose.
10. barrier holder according to claim 9, which is characterized in that the aperture of the bacteria cellulose film is less than
100nm。
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