CN107744602A - A kind of preparation method of bio-ink material available for 3D printing - Google Patents
A kind of preparation method of bio-ink material available for 3D printing Download PDFInfo
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- CN107744602A CN107744602A CN201710917939.8A CN201710917939A CN107744602A CN 107744602 A CN107744602 A CN 107744602A CN 201710917939 A CN201710917939 A CN 201710917939A CN 107744602 A CN107744602 A CN 107744602A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0023—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/0005—Ingredients of undetermined constitution or reaction products thereof
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0015—Medicaments; Biocides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0031—Hydrogels or hydrocolloids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/08—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0057—Ingredients of undetermined constitution or reaction products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0066—Medicaments; Biocides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y70/00—Materials specially adapted for additive manufacturing
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F251/00—Macromolecular compounds obtained by polymerising monomers on to polysaccharides or derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
Abstract
The invention discloses a kind of preparation method of the bio-ink material available for 3D printing, the preparation method mainly preparation including modified sodium alginate and the preparation of bio-ink material.A kind of preparation method of described bio-ink material available for 3D printing is by competent cell, cell culture medium, light trigger, modified sodium alginate and antioxidase composition.The bio-ink material of the present invention, sodium alginate is modified by photocuring, modified sodium alginate, the original good biocompatibility of sodium alginate, hemostatic can not only be kept, and due to connecting photo curable group on sodium alginate strand, the characteristic of dual crosslinking is made it have, can be fast curing-formed.This biomaterial has good biocompatibility with competent cell, and activity printing can be achieved, and thus the hydrogel of bio-ink material solidification shaping has good mechanical property, promoting healing function, hemostatic and biocompatibility.
Description
Technical field
The invention belongs to 3D printing field, and in particular to a kind of preparation side of bio-ink material available for 3D printing
Method.
Background technology
3D printing technique is an emerging manufacturing technology of rising in recent years, the technology mainly under the control of the computer,
The model converted after data is obtained according to CAD (CAD) model of printed matter or computed tomography (CT),
Pass through the accurate 3D accumulations of material, the digitlization forming technique of the quick 3D objects for manufacturing designated shape.
So far, the product manufactured using 3D printing technique is mostly used in et al. Ke organ, is repaiied for soft tissue
Recover and face very big challenge, wherein the biomaterial that research and development are suitable for 3D printing is crucial.
The problems such as long healing cycle, easy scar hyperplasia during reparation be present in soft tissue, the hydrogel that occurs at present,
Although the high-end medical dressing such as foam dressing, hydrocolloid, alginates medical films can reduce the cycle of soft tissue healing, effect
It is limited.
Using the 3D printing technique of biologic medical it is widely used be that synthetic material includes the degradable absorption polyester of thermoplasticity
Plastics (PLA, PLGA, PCL and their copolymer), and some natural macromolecular material (alginate, fiber eggs
In vain, collagen, gelatin, chitosan, hyaluronic acid etc., above-mentioned synthetic material carry out 3D using organic solvent or high temperature mostly and beaten
Print, the supporting structure printed is stable, but limited precision, it is impossible to is printed with mixing with cells.Water miscible natural macromolecular material
It can be printed simultaneously with cell, but cytoactive, support mechanical property and biocompatibility are poor, and it is fast curing-formed relatively tired
It is difficult.Current material be used alone or it is compound be all difficult to take into account cell compatibility, bioactivity and mechanical property, quick solid
Chemical conversion type.Therefore, bio-ink, it is the difficulty all not solved very well so far in particular for the bio-ink of soft tissue repair
Topic.
The content of the invention
The present invention provides a kind of preparation method of the bio-ink material available for 3D printing regarding to the issue above, the present invention
Bio-ink material, sodium alginate is modified by photocuring, modified sodium alginate, can not only keep sodium alginate
It is original good biocompatibility, hemostatic, and due to connecting photo curable group on sodium alginate strand, make it
Characteristic with dual crosslinking, can be fast curing-formed.This biomaterial has good biocompatibility with competent cell, can
Activity printing is realized, thus the hydrogel of bio-ink material solidification shaping has good mechanical property, promoting healing function, stopped
Courageous and upright and biocompatibility.
The present invention adopts the technical scheme that：A kind of preparation method of bio-ink material available for 3D printing, it is special
Sign is, comprises the following steps：
（1）The preparation of modified sodium alginate
A certain amount of sodium alginate is weighed in 25 ~ 50 DEG C of distilled water to being completely dissolved, then by sodium alginate and modifying agent
Mass ratio be 1:10 ~ 20 are matched, and after stirring 0.5 ~ 1h, reactant is adjusted with the NaOH solution that mass percent is 1%
The pH of system to 8 ~ 9,48 h are then reacted under the conditions of 4 DEG C, the NaOH that constantly addition mass percent is 1% during reaction is maintained
The pH of reaction system is eventually adding in bag filter 48 h that dialyse between 8.5, is freeze-dried, after irradiation sterilization, produces modified sea
Mosanom；
（2）The preparation of bio-ink material
A certain amount of modified sodium alginate and light trigger are dissolved in cell culture medium successively, produce A liquid；Competent cell is existed
After normally cultivating for 4 ~ 8 generations in cell culture medium, certain density cell suspending liquid is configured to, then adds antioxidase, stirred
Uniformly, the cell suspending liquid containing antioxidase is configured to, produces B liquid；Then A liquid and B liquid are well mixed, produce a kind of biology
Ink material.
Further, the step（1）Middle modifying agent is GMA.In the strand of sodium alginate
On connect can be with the group GMA of photocuring, keeping, the original good biological of sodium alginate is compatible
While property, hemostatic and absorbency, ionomer and the dual friendship of photocuring can be made it have with the group of photocuring by adding
The characteristic of connection.
Further, the step（1）The molecular cut off of bag filter is 14000 Da.The modified sea of different molecular quality
Mosanom, the mechanical property after solidification, biocompatibility and degradability are different, by controlling the molecular cut off of bag filter favourable
In the modified sodium alginate for preparing certain molecular weight scope, so as to control the quality of modified sodium alginate.
Further, the step（1）The substitution value of modified sodium alginate is 35 ~ 60%.Control modified sodium alginate takes
Dai Du, the original good biocompatibility of sodium alginate, hemostatic and absorbency can be not only kept, and ion can be kept
Crosslinking and the characteristic of the dual crosslinking of photocuring.
Further, the step（1）The dosage of middle irradiation sterilization is 15 ~ 25KGY.Modified sodium alginate is carried out
After sterilizing, the pathogenic bacteria of the inside can be killed, when being prepared into bio-ink material, are advantageous to improve living in bio-ink material
The survival rate of cell.
Further, the step（1）（2）Middle competent cell, light trigger, modified sodium alginate, antioxidase with it is thin
The mass ratio of born of the same parents' culture medium is（1~3）：（1~3）：（2~8）：（2~10）：（35~90）.
Further, the step（2）The mass ratio of light trigger and modified sodium alginate and cell culture medium in middle A liquid
For（1~3）：（2~8）：（20~55）.
Further, the step（2）Middle light trigger is azo dimethyl n-2- hydroxyl butyl propionamides.Add light-initiated
Agent, be advantageous to the fixation of modified sodium alginate.
Further, the step（2）Middle antioxidase is catalase.During photocuring, it can produce
Substantial amounts of free radical, free radical have very big destruction to living cells, can significantly reduce cell during 3D printing
Survival rate.Elimination effect of the catalase to free radical is very strong, advantageously reduces damage of the free radical to cell, improves thin
The survival rate of born of the same parents.
Further, the step（2）Middle competent cell is basic fibroblast.Basic fibroblast is cured with wound
Close relevant, the healing of the surface of a wound can be remarkably promoted.
Beneficial effects of the present invention are：
（1）Sea can kept with the group GMA of photocuring by being connected on the strand of sodium alginate
While the original good biocompatibility of mosanom, hemostatic and absorbency, its tool can be made with the group of photocuring by adding
There is the characteristic of ionomer and the dual crosslinking of photocuring, can be fast curing-formed, it is suitable quick can to solve current 3D printing shortage
The problem of shaped hydrogel.
（2）Elimination effect of the catalase added in bio-ink material to free radical is very strong, advantageously reduces
Damage of the free radical to cell, the survival rate of cell is improved, realize the good compound of biomaterial and cell, realize that activity is beaten
Print.
（3）Competent cell is added in bio-ink material, the medical dressing come out by 3D printing has to be promoted well
Healing effect, the healing of the surface of a wound can be remarkably promoted.
（4）The medical dressing prepared by the bio-ink material has good mechanical property, promoting healing effect, hemostasis
Property and biocompatibility.
Embodiment
Technical scheme is described further with reference to embodiment.
Embodiment 1
A kind of preparation method of bio-ink material available for 3D printing, comprises the following steps：
（1）The preparation of modified sodium alginate
2 parts of sodium alginates are weighed in 100 parts 35 DEG C of distilled water to being completely dissolved, then add 15 parts of methacrylic acid contractings
Water glyceride, after stirring 45min, the pH to 8.0 of reaction system is adjusted with the NaOH solution that mass percent is 1%, then 4
48 h are reacted under the conditions of DEG C, constantly the pH for the NaOH maintenance reaction systems that addition mass percent is 1% is 8.0 during reaction, most
Dialyse 48 hs are added in bag filter afterwards, and the molecular cut off of bag filter is 14000 Da, freeze-drying, is irradiated under the conditions of 15 KGY
After sterilizing, modified sodium alginate is produced.The substitution value of modified sodium alginate prepared by the method is 35%；
（2）The preparation of bio-ink material
2 parts of modified sodium alginates and 1 part of azo dimethyl n-2- hydroxyl butyl propionamide are dissolved in 20 parts of cell culture mediums successively
In, produce A liquid；After 1 part of basic fibroblast normally is cultivated into 4 generations in 15 parts of cell culture mediums, finite concentration is configured to
Cell suspending liquid, then add 2 parts of catalases, stir, be configured to the cell suspending liquid containing catalase, i.e.,
Obtain B liquid；Then A liquid and B liquid are well mixed, produce a kind of bio-ink material.
The bio-ink material is printed by 3D printer, photo-crosslinking can be solidified in 6s under 365nm ultraviolet irradiations
Shaping, by the hydrogel of curing molding it is clipped, packaging, sterilizing, used with 150 patient with diabetic feet, 96% patient
The surface of a wound heals completely after 15 days, and is produced without scar, and control group is alginates medical films（Purchase Xerox's brightness product）, control group
150 patient with diabetic feet wound healing cycles are 35 days.According to GB/T 6344-2008 method, the stretching to hydrogel is strong
Degree and elongation at break are detected, and experiment measures tensile strength >=1.52MPa of hydrogel, elongation at break >=43.3%.
Embodiment 2
A kind of preparation method of bio-ink material available for 3D printing, comprises the following steps：
（1）The preparation of modified sodium alginate
2 parts of sodium alginates are weighed in 100 parts 35 DEG C of distilled water to being completely dissolved, then add 15 parts of Glycidyl methacrylates
Glyceride, after stirring 45min, the pH to 8.5 of reaction system is adjusted with the NaOH solution that mass percent is 1%, then at 4 DEG C
Under the conditions of react 48 h, constantly the pH for the NaOH maintenance reaction systems that addition mass percent is 1% is 8.5 during reaction, finally
48 h that dialyse are added in bag filter, and the molecular cut off of bag filter is 14000 Da, freeze-drying, irradiates and goes out under the conditions of 20 KGY
After bacterium, modified sodium alginate is produced.The substitution value of modified sodium alginate prepared by the method is 50%；
（2）The preparation of bio-ink material
5 parts of modified sodium alginates and 2 parts of azo dimethyl n-2- hydroxyl butyl propionamides are dissolved in 40 parts of cell culture mediums successively
In, produce A liquid；After 2 parts of basic fibroblasts normally are cultivated into 6 generations in 30 parts of cell culture mediums, finite concentration is configured to
Cell suspending liquid, then add 6 parts of catalases, stir, be configured to the cell suspending liquid containing catalase, i.e.,
Obtain B liquid；Then A liquid and B liquid are well mixed, produce a kind of bio-ink material.
The bio-ink material is printed by 3D printer, can be consolidated photo-crosslinking in 4.5s under 365nm ultraviolet irradiations
Chemical conversion type, by the hydrogel of curing molding it is clipped, packaging, sterilizing, used with 150 patient with diabetic feet, 97% trouble
Person after 13 days the surface of a wound heal completely, and without scar produce, control group is alginates medical films（Purchase Xerox's brightness product）, control group
150 patient with diabetic feet wound healing cycles are 35 days.According to GB/T 6344-2008 method, the stretching to hydrogel is strong
Degree and elongation at break are detected, and experiment measures tensile strength >=1.67MPa of hydrogel, elongation at break >=49.7%.
Embodiment 3
A kind of preparation method of bio-ink material available for 3D printing, comprises the following steps：
（1）The preparation of modified sodium alginate
2 parts of sodium alginates are weighed in 100 parts 50 DEG C of distilled water to being completely dissolved, then add 20 parts of Glycidyl methacrylates
Glyceride, after stirring 1h, the pH to 9.0 of reaction system is adjusted with the NaOH solution that mass percent is 1%, then in 4 DEG C of conditions
48 h of lower reaction, constantly the pH for the NaOH maintenance reaction systems that addition mass percent is 1% 9.0, is eventually adding during reaction
Dialyse 48 h in bag filter, and the molecular cut off of bag filter is 14000 Da, freeze-drying, irradiation sterilization under the conditions of 25 KGY
Afterwards, modified sodium alginate is produced.The substitution value of modified sodium alginate prepared by the method is 60%；
（2）The preparation of bio-ink material
8 parts of modified sodium alginates and 3 parts of azo dimethyl n-2- hydroxyl butyl propionamides are dissolved in 55 parts of cell culture mediums successively
In, produce A liquid；After 3 parts of basic fibroblasts normally are cultivated into 8 generations in 35 parts of cell culture mediums, finite concentration is configured to
Cell suspending liquid, then add 10 parts of catalases, stir, be configured to the cell suspending liquid containing catalase,
Produce B liquid；Then A liquid and B liquid are well mixed, produce a kind of bio-ink material.
The bio-ink material is printed by 3D printer, photo-crosslinking can be solidified in 3s under 365nm ultraviolet irradiations
Shaping, by the hydrogel of curing molding it is clipped, packaging, sterilizing, used with 150 patient with diabetic feet, 98% patient
The surface of a wound heals completely after 10 days, and is produced without scar, and control group is alginates medical films（Purchase Xerox's brightness product）, control group
150 patient with diabetic feet wound healing cycles are 35 days.According to GB/T 6344-2008 method, the stretching to hydrogel is strong
Degree and elongation at break are detected, and experiment measures tensile strength >=2.35MPa of hydrogel, elongation at break >=56.4%.
Embodiment 4
A kind of preparation method of bio-ink material available for 3D printing, comprises the following steps：
（1）The preparation of modified sodium alginate
2 parts of sodium alginates are weighed in 100 parts 50 DEG C of distilled water to being completely dissolved, then add 20 parts of Glycidyl methacrylates
Glyceride, after stirring 1h, the pH to 9.0 of reaction system is adjusted with the NaOH solution that mass percent is 1%, then in 4 DEG C of conditions
48 h of lower reaction, constantly the pH for the NaOH maintenance reaction systems that addition mass percent is 1% 9.0, is eventually adding during reaction
Dialyse 48 h in bag filter, and the molecular cut off of bag filter is 14000 Da, freeze-drying, irradiation sterilization under the conditions of 25 KGY
Afterwards, modified sodium alginate is produced.The substitution value of modified sodium alginate prepared by the method is 60%；
（2）The preparation of bio-ink material
8 parts of modified sodium alginates and 3 parts of azo dimethyl n-2- hydroxyl butyl propionamides are dissolved in 55 parts of cell culture mediums successively
In, produce A liquid；After 3 parts of basic fibroblasts normally are cultivated into 8 generations in 35 parts of cell culture mediums, finite concentration is configured to
Cell suspending liquid, then add 10 parts of catalases, stir, be configured to the cell suspending liquid containing catalase,
Produce B liquid；Then A liquid and B liquid are well mixed, produce a kind of bio-ink material.
The bio-ink material is printed by 3D printer, soaked in the calcium chloride solution that mass concentration is 0.25%,
Curable shaping after 30s, the hydrogel of curing molding is washed through alcohol, distilled water cleaning, dry tack free, shearing, packaging, sterilizing,
Used with 150 patient with diabetic feet, 98% patient after 10 days the surface of a wound heal completely, and produced without scar, control group is
Alginates medical films（Purchase Xerox's brightness product）, 150 patient with diabetic feet wound healing cycles of control group are 35 days.According to
GB/T 6344-2008 method, the tensile strength and elongation at break of hydrogel are detected, experiment measures hydrogel
Tensile strength >=2.65MPa, elongation at break >=59.1%.
Embodiment 5
It is 2 × 10 by concentration for the bio-ink material no cytotoxicity of the checking present invention4Individual/mL cell suspension inoculation in
3 piece of 96 well culture plate, per the uL of hole 100, it is placed in 5%CO2, 24 h of culture in 37 DEG C of incubator.Original is abandoned after observation cell attachment
Liquid, washed 2 times with PBS, abandon after stoste be separately added into experimental group (sample sets), negative control group (10% hyclone DMEM) with
Positive controls (5% dimethyl sulfoxide (DMSO)), zeroing hole is set（Without cell, only plus culture medium, other steps are identical）It is each per hole
200 μ L, every group of 6 holes/plate, by 96 orifice plates after processing, after being placed in 5%CO2,37 DEG C of h of incubator culture 72, observe cell shape
State and growing state.Take out one piece of 96 well culture plate respectively in above-mentioned time point, being separately added into 20 μ L MTT, (mass concentration is
5 mg/mL, now with the current), 5%CO2, 37 DEG C be incubated 4 h after terminate culture.Add 200 μ L's per hole after sucking supernatant
DMSO, 600 rpm/min vibrate 10 min, and ELIASA determines absorbance in 490 nm, and records result.Calculate relative propagation
Rate (RGR), toxicity of the evaluation material to cell.Cell is calculated as follows with respect to appreciation rate：
RGR（%）=（Experimental group OD values/negative control group OD values）×100%
The bio-ink material of table 1 and cell growth status after the h of cells contacting 72
As it can be seen from table 1 positive controls have very maxicell toxicity, 1 ~ 4 equal acellular poison of negative control group and embodiment
Property.As a result show, bio-ink material prepared by the present invention has good biocompatibility, no cytotoxicity.
It should be pointed out that embodiment is the more representational example of the present invention, it is clear that skill of the invention
Art scheme is not limited to above-described embodiment, can also there is many variations.One of ordinary skill in the art, it is clearly public with institute of the invention
Written description open or according to file is undoubted to be obtained, and is considered as this patent scope of the claimed.
Claims (10)
1. the preparation method of a kind of bio-ink material available for 3D printing, it is characterised in that comprise the following steps：
（1）The preparation of modified sodium alginate：
A certain amount of sodium alginate is weighed in 25 ~ 50 DEG C of distilled water to being completely dissolved, then by sodium alginate and modifying agent
Mass ratio be 1:10 ~ 20 are matched, and after stirring 0.5 ~ 1h, reactant is adjusted with the NaOH solution that mass percent is 1%
The pH of system to 8 ~ 9,48 h are then reacted under the conditions of 4 DEG C, the NaOH that constantly addition mass percent is 1% during reaction is maintained
The pH of reaction system is eventually adding in bag filter 48 h that dialyse between 8.5, is freeze-dried, after irradiation sterilization, produces modified sea
Mosanom；
（2）The preparation of bio-ink material：
A certain amount of modified sodium alginate and light trigger are dissolved in cell culture medium successively, produce A liquid；Competent cell is existed
After normally cultivating for 4 ~ 8 generations in cell culture medium, certain density cell suspending liquid is configured to, then adds antioxidase, stirred
Uniformly, the cell suspending liquid containing antioxidase is configured to, produces B liquid；Then A liquid and B liquid are well mixed, produce a kind of biology
Ink material.
A kind of 2. preparation method of bio-ink material available for 3D printing according to claim 1, it is characterised in that
The step（1）Middle modifying agent is GMA.
A kind of 3. preparation method of bio-ink material available for 3D printing according to claim 1, it is characterised in that
The step（1）The molecular cut off of bag filter is 14000 Da.
A kind of 4. preparation method of bio-ink material available for 3D printing according to claim 1, it is characterised in that
The step（1）The substitution value of modified sodium alginate is 35 ~ 60%.
A kind of 5. preparation method of bio-ink material available for 3D printing according to claim 1, it is characterised in that
The step（1）The dosage of middle irradiation sterilization is 15 ~ 25KGY.
A kind of 6. preparation method of bio-ink material available for 3D printing according to claim 1, it is characterised in that
The step（1）（2）Middle competent cell, light trigger, modified sodium alginate, the mass ratio of antioxidase and cell culture medium are
（1~3）：（1~3）：（2~8）：（2~10）：（35~90）.
A kind of 7. preparation method of bio-ink material available for 3D printing according to claim 1, it is characterised in that
The step（2）The mass ratio of light trigger and modified sodium alginate and cell culture medium is in middle A liquid（1~3）：（2~8）：（20
~55）.
A kind of 8. preparation method of bio-ink material available for 3D printing according to claim 1, it is characterised in that
The step（2）Middle light trigger is azo dimethyl n-2- hydroxyl butyl propionamides.
A kind of 9. preparation method of bio-ink material available for 3D printing according to claim 1, it is characterised in that
The step（2）Middle antioxidase is catalase.
10. a kind of preparation method of bio-ink material available for 3D printing according to claim 1, its feature exist
In the step（2）Middle competent cell is basic fibroblast.
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| CN115998954A (en) * | 2021-10-22 | 2023-04-25 | 重庆医科大学附属口腔医院 | Biological ink composition containing giant salamander skin secretion and application thereof |
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| CN112451746A (en) * | 2020-11-25 | 2021-03-09 | 广东省科学院生物工程研究所 | Preparation method of light-curable sodium alginate hydrogel repair stent |
| CN113842506A (en) * | 2021-08-13 | 2021-12-28 | 广东唯泰生物科技有限公司 | Biological ink for 3D biological printing and preparation method and application thereof |
| CN113679888A (en) * | 2021-08-26 | 2021-11-23 | 山东大学 | Photocuring forming composite hydrogel matrix precursor, preparation method thereof and stent with same |
| CN115998954A (en) * | 2021-10-22 | 2023-04-25 | 重庆医科大学附属口腔医院 | Biological ink composition containing giant salamander skin secretion and application thereof |
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