CN108542938B - Antibacterial agent - Google Patents
Antibacterial agent Download PDFInfo
- Publication number
- CN108542938B CN108542938B CN201810475053.7A CN201810475053A CN108542938B CN 108542938 B CN108542938 B CN 108542938B CN 201810475053 A CN201810475053 A CN 201810475053A CN 108542938 B CN108542938 B CN 108542938B
- Authority
- CN
- China
- Prior art keywords
- parts
- chinese medicine
- traditional chinese
- antibacterial agent
- stock solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
- A61K31/047—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates having two or more hydroxy groups, e.g. sorbitol
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/13—Amines
- A61K31/14—Quaternary ammonium compounds, e.g. edrophonium, choline
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/538—Schizonepeta
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/62—Nymphaeaceae (Water-lily family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/04—Antipruritics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Dermatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicines Containing Plant Substances (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses the technical field of antibiosis and inflammation diminishing, and particularly relates to an antibacterial agent which comprises, by weight, 30-40 parts of pomegranate rind, 22-32 parts of radix sophorae flavescentis, 20-30 parts of lotus leaves, 17-27 parts of elsholtzia, 15-25 parts of schizonepeta, 15-25 parts of oregano, 13-23 parts of fructus anethi, 10-20 parts of indigo naturalis, 10-20 parts of rosemary, 20-30 parts of glycerol, 15-25 parts of PEG-40 hydrogenated castor oil, 2-5 parts of dodecyl dimethyl benzyl ammonium chloride, 0.5-2 parts of tea tree essential oil and 55-65 parts of purified water.
Description
Technical Field
The invention relates to the technical field of antibiosis and antiphlogosis, in particular to an antibacterial agent.
Background
The antibacterial agent is a chemical substance which can keep the growth or reproduction of certain microorganisms (fungi, bacteria, yeasts, algae, viruses and the like) below a necessary level within a certain period of time, and has antibacterial and bactericidal properties, and is generally divided into an inorganic antibacterial agent, an organic antibacterial agent and a natural antibacterial agent, wherein the inorganic antibacterial agent is mainly a silver ion antibacterial agent, and has the advantages of high safety and high cost and complex manufacturing process; the organic antibacterial agent is generally vanillin or ethyl vanillin compound, and has the advantages of rapid sterilization, strong sterilization capability, short effective period, easy decomposition and incomplete and definite safety; the natural antibacterial agent mainly comprises chitin, castor oil, etc., and has the advantages of simple use, high safety, limited antibacterial effect and low bactericidal rate. The traditional natural antibacterial agent has limited antibacterial effect, and the organic antibacterial agent has short effective period and cannot ensure safety, so that the invention provides the antibacterial agent, which is prepared by extracting and combining safe low-concentration chemical antibacterial substances from traditional Chinese medicines, so that the antibacterial agent can quickly inhibit bacteria and can inhibit bacteria for a long time.
Disclosure of Invention
The present invention has been made to solve the above problems occurring in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: an antibacterial agent characterized by: the antibacterial agent consists of the following components:
pomegranate rind, radix sophorae flavescentis, lotus leaves, elsholtzia, schizonepeta, origanum vulgaris, Chinese fennel, indigo naturalis, rosemary, glycerin, PEG-40 hydrogenated castor oil, dodecyl dimethyl benzyl ammonium chloride, tea tree essential oil and purified water.
Preferably, the antibacterial agent consists of the following components in parts by weight:
30-40 parts of pomegranate bark, 22-32 parts of radix sophorae flavescentis, 20-30 parts of lotus leaves, 17-27 parts of elsholtzia, 15-25 parts of schizonepeta, 15-25 parts of oregano, 13-23 parts of fructus illicii, 10-20 parts of indigo naturalis, 10-20 parts of rosemary, 20-30 parts of glycerol, 15-25 parts of PEG-40 hydrogenated castor oil, 2-5 parts of dodecyl dimethyl benzyl ammonium chloride, 0.5-2 parts of tea tree essential oil and 55-65 parts of purified water.
Preferably, the preparation method of the antibacterial agent comprises the following steps:
1) crushing radix sophorae flavescentis, elsholtzia, Chinese fennel and rosemary by using a traditional Chinese medicine crusher, sieving by using a 40-mesh sieve to obtain traditional Chinese medicine powder A, placing the traditional Chinese medicine powder A into a supercritical carbon dioxide extraction kettle, extracting the traditional Chinese medicine powder A by using carbon dioxide, introducing the carbon dioxide at the flow rate of 18-25 liters/hour, the extraction pressure of 15-30 MPa and the extraction temperature of 35-50 ℃, centrifuging by using a centrifuge after extracting for 1 hour, and taking supernatant to obtain traditional Chinese medicine stock solution A for later use;
2) crushing pomegranate rind, lotus leaf, schizonepeta, oregano and indigo naturalis by using a traditional Chinese medicine crusher, sieving by using a 40-mesh sieve to obtain traditional Chinese medicine powder B, adding purified water with the volume of 10 times into the traditional Chinese medicine powder B, soaking for 1 hour, decocting for 2 hours by using a micro-pressure decocting machine for 3 times, filtering and combining filtrates, and evaporating and concentrating to obtain traditional Chinese medicine stock solution B for later use;
3) heating glycerol in a water bath to 45-65 ℃, adding the traditional Chinese medicine stock solution A, the traditional Chinese medicine stock solution B and the tea tree essential oil, fully mixing, cooling to 35-45 ℃, adding dodecyl dimethyl benzyl ammonium chloride into the mixture, continuously stirring by using a stirrer to fully mix the mixture, adding PEG-40 hydrogenated castor oil, stirring at a constant speed to obtain a mixed solution, adding purified water, and uniformly stirring to obtain the antibacterial agent.
Compared with the prior art, the invention has the beneficial effects that: the antibacterial agent is prepared by compounding a traditional Chinese medicine extract with bacteriostasis and antibiosis and a safe low-concentration chemical bacteriostatic substance, has the advantages of easily obtained raw materials, reasonable formula, quick sterilization, long-acting bacteriostasis, mildness and no stimulation, has better bacteriostasis and sterilization effects on pseudomonas aeruginosa by pomegranate peel, and can prevent postoperative and wound infection; the fructus Foeniculi has active ingredients such as monoterpene and terpene alcohol compounds and volatile alkaloid, and has strong inhibiting effect on Staphylococcus aureus, Diplococcus pneumoniae, Escherichia coli, Bacillus dysenteriae and Pseudomonas aeruginosa; the marjoram is a natural antibiotic, and can prevent influenza, sterilize and inhibit bacteria; in addition, the elsholtzia and the radix sophorae flavescentis also have good anti-inflammatory and bacteriostatic effects; dodecyl dimethyl benzyl ammonium chloride is a cationic surfactant, is a non-oxidative bactericide and has the capability of broad-spectrum efficient sterilization and algae killing, and lotus leaves, schizonepeta and indigo naturalis have the effects of clearing away heat and toxic materials, dispelling wind and cooling blood and relieving itching; rosemary is an oxidant with excellent oxidation resistance, and rosemary essential oil has the effects of tranquilizing and allaying excitement, refreshing brain, disinfecting and sterilizing; the tea tree essential oil can remove peculiar smell; the glycerin has good moistening effect. The antibacterial agent can be used for quickly sterilizing, inhibiting bacteria for a long time, clearing away heat and toxic materials, dispelling wind and relieving itching, and removing peculiar smell, and is moistening and non-irritating.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the antibacterial agent comprises the following raw materials in weight gram: 30 g of pomegranate bark, 22 g of radix sophorae flavescentis, 20 g of lotus leaf, 17 g of elsholtzia, 15 g of schizonepeta, 15 g of oregano, 13 g of fennel, 10 g of natural indigo, 10 g of rosemary, 20 g of glycerin, 15 g of PEG-40 hydrogenated castor oil, 2 g of dodecyl dimethyl benzyl ammonium chloride, 0.5 g of tea tree essential oil and 55 g of purified water.
The preparation process of the antibacterial agent comprises the following steps: extracting radix Sophorae Flavescentis, herba Moslae, fructus Foeniculi and herba Rosmarini officinalis of 40 mesh with supercritical carbon dioxide extraction kettle at flow rate of 18L/hr and extraction pressure of 15 MPa at 35 deg.C for 1 hr, and centrifuging to obtain Chinese medicinal stock solution A;
soaking 40-mesh pericarpium Granati, folium Nelumbinis, herba Schizonepetae, Oregano, and indigo naturalis powder in 10 times volume of purified water for 1 hr, decocting for 2 hr with a micropressure decocting machine for 3 times, filtering, and concentrating to obtain Chinese medicinal stock solution B;
heating glycerol in water bath to 45 ℃, adding the traditional Chinese medicine stock solution A, the traditional Chinese medicine stock solution B and the tea tree essential oil, fully mixing, cooling to 35 ℃, adding dodecyl dimethyl benzyl ammonium chloride into the mixture, continuously stirring by using a stirrer to fully mix the mixture, adding PEG-40 hydrogenated castor oil, stirring at a constant speed to obtain a mixed solution, adding purified water, and uniformly stirring to obtain the antibacterial agent.
Example 2:
the antibacterial agent comprises the following raw materials in weight gram: 32 g of pomegranate bark, 24 g of radix sophorae flavescentis, 22 g of lotus leaf, 19 g of elsholtzia, 17 g of schizonepeta, 17 g of oregano, 15 g of fennel, 12 g of natural indigo, 12 g of rosemary, 22 g of glycerin, 17 g of PEG-40 hydrogenated castor oil, 3 g of dodecyl dimethyl benzyl ammonium chloride, 0.8 g of tea tree essential oil and 57 g of purified water.
The preparation process of the antibacterial agent comprises the following steps: extracting radix Sophorae Flavescentis, herba Moslae, fructus Foeniculi and herba Rosmarini officinalis of 40 mesh with supercritical carbon dioxide extraction kettle at flow rate of 20L/hr and extraction pressure of 18 MPa at 38 deg.C for 1 hr, and centrifuging to obtain Chinese medicinal stock solution A;
soaking 40-mesh pericarpium Granati, folium Nelumbinis, herba Schizonepetae, Oregano, and indigo naturalis powder in 10 times volume of purified water for 1 hr, decocting for 2 hr with a micropressure decocting machine for 3 times, filtering, and concentrating to obtain Chinese medicinal stock solution B;
heating glycerol in water bath to 49 ℃, adding the traditional Chinese medicine stock solution A, the traditional Chinese medicine stock solution B and the tea tree essential oil, fully mixing, cooling to 37 ℃, adding dodecyl dimethyl benzyl ammonium chloride into the mixture, continuously stirring by using a stirrer to fully mix the mixture, adding PEG-40 hydrogenated castor oil, stirring at a constant speed to obtain a mixed solution, adding purified water, and uniformly stirring to obtain the antibacterial agent.
Example 3:
the antibacterial agent comprises the following raw materials in weight gram: 34 g of pomegranate bark, 26 g of radix sophorae flavescentis, 24 g of lotus leaf, 21 g of elsholtzia, 19 g of schizonepeta, 19 g of oregano, 17 g of fennel, 14 g of natural indigo, 14 g of rosemary, 24 g of glycerin, 19 g of PEG-40 hydrogenated castor oil, 3.5 g of dodecyl dimethyl benzyl ammonium chloride, 1.1 g of tea tree essential oil and 59 g of purified water.
The preparation process of the antibacterial agent comprises the following steps: extracting radix Sophorae Flavescentis, herba Moslae, fructus Foeniculi and herba Rosmarini officinalis of 40 mesh with supercritical carbon dioxide extraction kettle at flow rate of 22L/hr and extraction pressure of 23 MPa at 42 deg.C for 1 hr, and centrifuging to obtain Chinese medicinal stock solution A;
soaking 40-mesh pericarpium Granati, folium Nelumbinis, herba Schizonepetae, Oregano, and indigo naturalis powder in 10 times volume of purified water for 1 hr, decocting for 2 hr with a micropressure decocting machine for 3 times, filtering, and concentrating to obtain Chinese medicinal stock solution B;
heating glycerol in water bath to 53 ℃, adding the traditional Chinese medicine stock solution A, the traditional Chinese medicine stock solution B and the tea tree essential oil, fully mixing, cooling to 39 ℃, adding dodecyl dimethyl benzyl ammonium chloride into the mixture, continuously stirring by using a stirrer to fully mix the mixture, adding PEG-40 hydrogenated castor oil, stirring at a constant speed to obtain a mixed solution, adding purified water, and uniformly stirring to obtain the antibacterial agent.
Example 4:
the antibacterial agent comprises the following raw materials in weight gram: 36 g of pomegranate bark, 28 g of radix sophorae flavescentis, 26 g of lotus leaf, 23 g of elsholtzia, 21 g of schizonepeta, 21 g of oregano, 19 g of fennel, 16 g of natural indigo, 16 g of rosemary, 26 g of glycerin, 21 g of PEG-40 hydrogenated castor oil, 3.9 g of dodecyl dimethyl benzyl ammonium chloride, 1.4 g of tea tree essential oil and 61 g of purified water.
The preparation process of the antibacterial agent comprises the following steps: extracting radix Sophorae Flavescentis, herba Moslae, fructus Foeniculi and herba Rosmarini officinalis of 40 mesh with supercritical carbon dioxide extraction kettle at flow rate of 23L/hr and extraction pressure of 26 MPa at 45 deg.C for 1 hr, and centrifuging to obtain Chinese medicinal stock solution A;
soaking 40-mesh pericarpium Granati, folium Nelumbinis, herba Schizonepetae, Oregano, and indigo naturalis powder in 10 times volume of purified water for 1 hr, decocting for 2 hr with a micropressure decocting machine for 3 times, filtering, and concentrating to obtain Chinese medicinal stock solution B;
heating glycerol in water bath to 58 ℃, adding the traditional Chinese medicine stock solution A, the traditional Chinese medicine stock solution B and the tea tree essential oil, fully mixing, cooling to 41 ℃, adding dodecyl dimethyl benzyl ammonium chloride into the mixture, continuously stirring by using a stirrer to fully mix the mixture, adding PEG-40 hydrogenated castor oil, stirring at a constant speed to obtain a mixed solution, adding purified water, and uniformly stirring to obtain the antibacterial agent.
Example 5:
the antibacterial agent comprises the following raw materials in weight gram: 38 g of pomegranate rind, 30 g of radix sophorae flavescentis, 28 g of lotus leaf, 25 g of elsholtzia, 23 g of schizonepeta, 23 g of oregano, 21 g of Chinese fennel, 18 g of natural indigo, 18 g of rosemary, 28 g of glycerin, 23 g of PEG-40 hydrogenated castor oil, 4.5 g of dodecyl dimethyl benzyl ammonium chloride, 1.7 g of tea tree essential oil and 63 g of purified water.
The preparation process of the antibacterial agent comprises the following steps: extracting radix Sophorae Flavescentis, herba Moslae, fructus Foeniculi and herba Rosmarini officinalis of 40 mesh with supercritical carbon dioxide extraction kettle at flow rate of 24L/hr, extraction pressure of 28 MPa and 47 deg.C for 1 hr, and centrifuging to obtain Chinese medicinal stock solution A;
soaking 40-mesh pericarpium Granati, folium Nelumbinis, herba Schizonepetae, Oregano, and indigo naturalis powder in 10 times volume of purified water for 1 hr, decocting for 2 hr with a micropressure decocting machine for 3 times, filtering, and concentrating to obtain Chinese medicinal stock solution B;
heating glycerol in water bath to 62 ℃, adding the traditional Chinese medicine stock solution A, the traditional Chinese medicine stock solution B and the tea tree essential oil, fully mixing, cooling to 43 ℃, adding dodecyl dimethyl benzyl ammonium chloride into the mixture, continuously stirring by using a stirrer to fully mix the mixture, adding PEG-40 hydrogenated castor oil, stirring at a constant speed to obtain a mixed solution, adding purified water, and uniformly stirring to obtain the antibacterial agent.
Example 6:
the antibacterial agent comprises the following raw materials in weight gram: 40 g of pomegranate bark, 32 g of radix sophorae flavescentis, 30 g of lotus leaf, 27 g of elsholtzia, 25 g of schizonepeta, 25 g of oregano, 23 g of fennel, 20 g of natural indigo, 20 g of rosemary, 30 g of glycerin, 25 g of PEG-40 hydrogenated castor oil, 5 g of dodecyl dimethyl benzyl ammonium chloride, 2 g of tea tree essential oil and 65 g of purified water.
The preparation process of the antibacterial agent comprises the following steps: extracting radix Sophorae Flavescentis, herba Moslae, fructus Foeniculi and herba Rosmarini officinalis of 40 mesh with supercritical carbon dioxide extraction kettle at flow rate of 24L/hr and extraction pressure of 30 MPa at 50 deg.C for 1 hr, and centrifuging to obtain Chinese medicinal stock solution A;
soaking 40-mesh pericarpium Granati, folium Nelumbinis, herba Schizonepetae, Oregano, and indigo naturalis powder in 10 times volume of purified water for 1 hr, decocting for 2 hr with a micropressure decocting machine for 3 times, filtering, and concentrating to obtain Chinese medicinal stock solution B;
heating glycerol in water bath to 65 ℃, adding the traditional Chinese medicine stock solution A, the traditional Chinese medicine stock solution B and the tea tree essential oil, fully mixing, cooling to 45 ℃, adding dodecyl dimethyl benzyl ammonium chloride into the mixture, continuously stirring by using a stirrer to fully mix the mixture, adding PEG-40 hydrogenated castor oil, stirring at a constant speed to obtain a mixed solution, adding purified water, and uniformly stirring to obtain the antibacterial agent.
And (3) efficacy verification: the trial detection effect is carried out on the products prepared in the embodiments 1-6;
test item 1: the invention carries out 30, 50 and 60 second sterilization effect detection on pseudomonas aeruginosa, staphylococcus aureus, escherichia coli, candida albicans and diplococcus pneumoniae, and the test results are shown in the following table 1:
the results show that the pseudomonas aeruginosa, staphylococcus aureus, escherichia coli, candida albicans and diplococcus pneumoniae can be quickly killed in the embodiments 1-6 of the invention within 30 seconds, the sterilization effect is obviously superior to that of the blank group, wherein the sterilization effect of the embodiment 5 is the best, and the sterilization rate of the pseudomonas aeruginosa, staphylococcus aureus, escherichia coli, candida albicans and diplococcus pneumoniae within 30 seconds reaches 99.8%.
Test item 2: the invention carries out long-acting bacteriostasis experiments for pseudomonas aeruginosa, staphylococcus aureus, escherichia coli, candida albicans and diplococcus pneumoniae for 0, 2 and 4 hours, and the test results are shown in the following table 2:
the results show that the antibacterial agents of the embodiments 1 to 6 have long-acting antibacterial effects on pseudomonas aeruginosa, staphylococcus aureus, escherichia coli, candida albicans and diplococcus pneumoniae, the antibacterial rate in four hours reaches 95%, the antibacterial effect of the embodiment 5 is the best, and the antibacterial rate on pseudomonas aeruginosa, staphylococcus aureus, escherichia coli, candida albicans and diplococcus pneumoniae reaches 98.1% in 4 hours.
Test item 3: in the embodiments 1 to 6 of the invention, the test experiments on the antipruritic effect, the odor removing effect, the moisturizing effect and the skin irritation are performed, 50 patients are selected for trial in the embodiments 1 to 6, and the test results are shown in the following table 3:
| example 1 | Example 2 | Example 3 | Example 4 | Example 5 | Example 6 | |
| The number of people with obvious itching relieving effect | 42 | 45 | 46 | 47 | 49 | 48 |
| The number of people with obvious odor removing effect | 47 | 48 | 48 | 49 | 50 | 49 |
| The number of people with obvious moistening effect | 41 | 43 | 45 | 47 | 49 | 49 |
| Irritation property | Is free of | Is free of | Is free of | Is free of | Is free of | Is free of |
The results show that the skin care cream disclosed by the embodiments 1-6 of the invention has good itching relieving effect, peculiar smell removing effect and moistening effect, and has no irritation to skin. The effect of example 5 is the best, 49 people feel obvious antipruritic effect, 50 people feel obvious peculiar smell removing effect, and 49 people feel obvious moisturizing effect after using the composition.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (1)
1. An antibacterial agent characterized by: the antibacterial agent is prepared from the following components:
30-40 parts of pomegranate peel, 22-32 parts of radix sophorae flavescentis, 20-30 parts of lotus leaf, 17-27 parts of elsholtzia, 15-25 parts of schizonepeta, 15-25 parts of oregano, 13-23 parts of fructus illicii, 10-20 parts of indigo naturalis, 10-20 parts of rosemary, 20-30 parts of glycerol, 15-25 parts of PEG-40 hydrogenated castor oil, 2-5 parts of dodecyl dimethyl benzyl ammonium chloride, 0.5-2 parts of tea tree essential oil and 55-65 parts of purified water;
the preparation method of the antibacterial agent comprises the following steps:
1) crushing radix sophorae flavescentis, elsholtzia, Chinese fennel and rosemary by using a traditional Chinese medicine crusher, sieving by using a 40-mesh sieve to obtain traditional Chinese medicine powder A, placing the traditional Chinese medicine powder A into a supercritical carbon dioxide extraction kettle, extracting the traditional Chinese medicine powder A by using carbon dioxide, introducing the carbon dioxide at the flow rate of 18-25 liters/hour, the extraction pressure of 15-30 MPa and the extraction temperature of 35-50 ℃, centrifuging by using a centrifuge after extracting for 1 hour, and taking supernatant to obtain traditional Chinese medicine stock solution A for later use;
2) crushing pomegranate rind, lotus leaf, schizonepeta, oregano and indigo naturalis by using a traditional Chinese medicine crusher, sieving by using a 40-mesh sieve to obtain traditional Chinese medicine powder B, adding purified water with the volume of 10 times into the traditional Chinese medicine powder B, soaking for 1 hour, decocting for 2 hours by using a micro-pressure decocting machine for 3 times, filtering and combining filtrates, and evaporating and concentrating to obtain traditional Chinese medicine stock solution B for later use;
3) heating glycerol in a water bath to 45-65 ℃, adding the traditional Chinese medicine stock solution A, the traditional Chinese medicine stock solution B and the tea tree essential oil, fully mixing, cooling to 35-45 ℃, adding dodecyl dimethyl benzyl ammonium chloride into the mixture, continuously stirring by using a stirrer to fully mix the mixture, adding PEG-40 hydrogenated castor oil, stirring at a constant speed to obtain a mixed solution, adding purified water, and uniformly stirring to obtain the antibacterial agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810475053.7A CN108542938B (en) | 2018-05-17 | 2018-05-17 | Antibacterial agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810475053.7A CN108542938B (en) | 2018-05-17 | 2018-05-17 | Antibacterial agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108542938A CN108542938A (en) | 2018-09-18 |
| CN108542938B true CN108542938B (en) | 2021-02-12 |
Family
ID=63494982
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810475053.7A Active CN108542938B (en) | 2018-05-17 | 2018-05-17 | Antibacterial agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108542938B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109105398A (en) * | 2018-09-21 | 2019-01-01 | 北京弘纳绿源科技发展有限公司 | A kind of plant source extraction bactericidal deodorant and preparation method thereof for environmental improvement |
| CN112210269A (en) * | 2019-07-09 | 2021-01-12 | 常州市大使涂料有限公司 | Nontoxic antibacterial coating for drinking water tank |
| CN110755477A (en) * | 2019-11-29 | 2020-02-07 | 中国科学院新疆理化技术研究所 | Application of schizonepeta tenuifolia essential oil and pomegranate bark effective component composition |
| CN112921430A (en) * | 2021-03-09 | 2021-06-08 | 广州泰达纤维制品有限公司 | Antibacterial warm-keeping cotton and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102021094A (en) * | 2009-09-22 | 2011-04-20 | 曹军 | Natural tea tree oil screen cleaning fluid |
| CN102319409A (en) * | 2003-07-17 | 2012-01-18 | 纽约市哥伦比亚大学托管会 | Antimicrobial compositons containing synergistic combinations of quaternary ammonium compounds and essential oils and/or constituents thereof |
| CN106912503A (en) * | 2015-12-25 | 2017-07-04 | 北京绿伞化学股份有限公司 | A kind of indoor air disinfection agent and preparation method thereof |
-
2018
- 2018-05-17 CN CN201810475053.7A patent/CN108542938B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102319409A (en) * | 2003-07-17 | 2012-01-18 | 纽约市哥伦比亚大学托管会 | Antimicrobial compositons containing synergistic combinations of quaternary ammonium compounds and essential oils and/or constituents thereof |
| CN102021094A (en) * | 2009-09-22 | 2011-04-20 | 曹军 | Natural tea tree oil screen cleaning fluid |
| CN106912503A (en) * | 2015-12-25 | 2017-07-04 | 北京绿伞化学股份有限公司 | A kind of indoor air disinfection agent and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 8种植物精油对肠道常见微生物体外抑菌效果的研究;吴克刚；等;《现代食品科技》;20170323;第33卷(第6期);133-141、93 * |
| 中药治疗足癣;曲浩源;《新疆中医药》;19900829(第4期);13 * |
| 关于中医治疗急性菌痢的苦干问题;时振声;《陕西新医药》;19790531;第8卷(第5期);34-36 * |
| 苦参石榴乌梅汤加味灌肠治疗小儿肠炎临床报道;刘飞霞;《黑龙江护理杂志》;20000912;第6卷(第9期);31页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108542938A (en) | 2018-09-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108542938B (en) | Antibacterial agent | |
| CN106334067B (en) | Gynecological gel containing silver ion antibacterial agent, preparation method and application | |
| JPH10298096A (en) | Synergistic herbal extract | |
| CN107412585B (en) | Green environment-friendly hand-washing-free disinfection gel and preparation method thereof | |
| CN106109534B (en) | Itching relieving repair liquid containing silver ion antibacterial agent, preparation method and application | |
| KR102366247B1 (en) | Wet wipe composition and its manufacturing method | |
| CN104922248B (en) | A kind of antibacterial, anti-inflammatory medicine compound gel and preparation method | |
| CN111150778A (en) | A sterilizing disinfectant with high safety and high efficiency for oral mucosa, skin and general articles, and its preparation method | |
| WO2020019929A1 (en) | Compound chinese traditional medicine antibacterial composition and application thereof in toiletry products | |
| JP2014533725A (en) | Skin preparation for external use with excellent antiseptic properties | |
| CN103933275A (en) | Antibacterial disinfection medicament and preparation method thereof | |
| KR20170130690A (en) | Natural detergent composition and preparation method thereof | |
| CN106138192B (en) | Bacteriostatic anti-inflammatory traditional Chinese medicine composition and detergent prepared from composition | |
| CN105012581A (en) | Fresh aloe antibacterial composite, application and preparing method of fresh aloe antibacterial composite | |
| Azubuike et al. | Formulation and evaluation of antimicrobial activities of herbal cream containing ethanolic extracts of Azadirachta indica leaves and Aloe vera gel | |
| CN109999128A (en) | The hundred antibacterial cream of grass of one kind | |
| KR101357168B1 (en) | A producing method for antimicrobial arm warmers | |
| CN104542739A (en) | Incense with anti-bacteria effect for clinical nursing and preparation method of incense | |
| CN101336966B (en) | Osmidrosis dispelling liniment containing natural essential oil of Zingiber corallinum Hance | |
| CN111481567B (en) | Compound ozone oil and preparation method and application thereof | |
| CN111821286B (en) | Liquid invisible mask with broad-spectrum bacteriostasis and preparation method and use method thereof | |
| CN104436116B (en) | A kind of rosemary lavipeditum powder and preparation method thereof | |
| CN113144058A (en) | Skin-moistening refreshing wash-free disinfection gel and preparation method thereof | |
| KR102140958B1 (en) | Method for manufacturing functional shampoo having antimicrobial effect containing peppermint, cinnamon, oregano, ginger Extract | |
| CN110711222A (en) | Compound essential oil for soothing skin and eczema of infants and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |