CN108526041A - Full-automatic male and female silkworm chrysalis sorting unit - Google Patents
Full-automatic male and female silkworm chrysalis sorting unit Download PDFInfo
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- CN108526041A CN108526041A CN201810288418.5A CN201810288418A CN108526041A CN 108526041 A CN108526041 A CN 108526041A CN 201810288418 A CN201810288418 A CN 201810288418A CN 108526041 A CN108526041 A CN 108526041A
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 160
- 230000007246 mechanism Effects 0.000 claims abstract description 55
- 230000007723 transport mechanism Effects 0.000 claims abstract description 26
- 238000001514 detection method Methods 0.000 claims abstract description 25
- 238000012216 screening Methods 0.000 claims abstract description 9
- 238000010362 genome editing Methods 0.000 claims abstract description 5
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 5
- 230000005540 biological transmission Effects 0.000 claims abstract description 4
- 241000382353 Pupa Species 0.000 claims description 68
- 238000004064 recycling Methods 0.000 claims description 30
- 230000003287 optical effect Effects 0.000 claims description 14
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 7
- 240000005373 Panax quinquefolius Species 0.000 claims description 7
- 230000029918 bioluminescence Effects 0.000 claims description 6
- 238000005415 bioluminescence Methods 0.000 claims description 6
- MURGITYSBWUQTI-UHFFFAOYSA-N fluorescin Chemical compound OC(=O)C1=CC=CC=C1C1C2=CC=C(O)C=C2OC2=CC(O)=CC=C21 MURGITYSBWUQTI-UHFFFAOYSA-N 0.000 claims description 6
- 230000009471 action Effects 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 2
- 230000008901 benefit Effects 0.000 abstract description 3
- 235000013601 eggs Nutrition 0.000 description 15
- 238000005516 engineering process Methods 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 208000037805 labour Diseases 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000006378 damage Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 238000010146 3D printing Methods 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000006830 Luminescent Proteins Human genes 0.000 description 1
- 108010047357 Luminescent Proteins Proteins 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000012053 enzymatic serum creatinine assay Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
- B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
- B07C5/34—Sorting according to other particular properties
- B07C5/342—Sorting according to other particular properties according to optical properties, e.g. colour
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
- B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
- B07C5/02—Measures preceding sorting, e.g. arranging articles in a stream orientating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
- B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
- B07C5/36—Sorting apparatus characterised by the means used for distribution
- B07C5/361—Processing or control devices therefor, e.g. escort memory
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
- B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
- B07C5/36—Sorting apparatus characterised by the means used for distribution
- B07C5/361—Processing or control devices therefor, e.g. escort memory
- B07C5/362—Separating or distributor mechanisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a kind of full-automatic male and female silkworm chrysalis sorting unit, the female silkworm chrysalis in the male and female silkworm chrysalis carries fluorescence protein gene after gene editing technical finesse, and the full-automatic male and female silkworm chrysalis sorting unit includes：Feed mechanism：Silkworm chrysalis to be sorted is set to fall one by one on the corresponding station of transport mechanism；Transport mechanism：Multiple stations are set, silkworm chrysalis to be sorted described in undertaking when for feed mechanism blanking, and the silkworm chrysalis to be sorted is transmitted；Detection module：In the transmission path of the transport mechanism, the detection identification that sorting silkworm chrysalis carries out male and female is treated, to confirm the gender of silkworm chrysalis to be sorted；Blanking mechanism：After completing silkworm chrysalis sex-screening by detection module in the transport mechanism, the silkworm chrysalis to be sorted in the transport mechanism falls within the blanking mechanism one by one；Sorting mechanism：According to the detection module to the sex-screening recognition result of the silkworm chrysalis to be sorted, silkworm chrysalis is sorted.The present invention has the advantages that efficient, accuracy rate is high.
Description
Technical field
The present invention relates to silkworm chrysalis sorting technology fields, and in particular to a kind of full-automatic male and female silkworm chrysalis sorting unit.
Background technology
Male and female silkworm chrysalis needs to match one by one to lay eggs, and no it will cause wastes, and male and female pairing means that and needs to sort.
Currently, Sex judging in silkworm egg production process is mainly by manually cutting cocoon and mirror pupa, labor intensity is big, low production efficiency.Kind
Cocoon Sex judging must be completed in 5~10 days, and silkworm egg enterprise has to recruit large quantities of short-term labours, such as the maximum silkworm egg in the whole nation
Shandong Guang Tong groups of manufacturing enterprise need to recruit 1000 people when reflecting pupa temporarily, and mirror pupa masterful technique worker is fewer and fewer.Silkworm chrysalis
The labor cost of Sex judging improves year by year, and with every 30 yuan of silkworm egg production cost, casual labour's cost is 7~8 yuan and (accounts for production
Cost 25% or so), wherein 1~2 yuan of silkworm chrysalis Sex judging labor cost (accounting for casual labour's cost 20% or so).Reflect pupa not only
It wants eyesight good, also to have certain technology that can distinguish female, male, have higher requirement, silkworm chrysalis Sex judging to the qualification of operation
Heavy workload, be pressed for time, technical staff lack have become the main problem that silkworm egg manufacturing enterprise faces.Meanwhile silkworm chrysalis male and female are reflected
Other accuracy rate is directly related to the purity of the quality and variety of silkworm egg, is provided according to Zhejiang Province quality of silkworm eggs quarantine and examination station
The silkworm egg hybrid rate average value survey data of annual 80~100 batch of 2005~2016 years the whole provinces, significantly decrease trend, from
Highest 99.66% drops to minimum 98.65%.The decline of silkworm egg hybrid rate will directly affect quality of silkworm eggs, silkworm chrysalis yield
With silk quality.When silkworm chrysalis is bred, it is necessary to be that the female moth of A kinds matches the male moth of B kinds.If occurring wrong choosing when male and female sort,
The female moth that just will appear A kinds matches the male moth of B kinds with the female moth of the male moth of A breedings or B kinds, and such words offspring is just
Have various diseases.Even only 5 percent falsely drop, will also result in breeding failure.So male and female sort, if cannot be female
Hero matches one by one, the problem of with regard to being not only waste problem, breeding can more seriously be caused to fail.
It can be seen that in the prior art, either manually sorting by rule of thumb or machine being according to silkworm chrysalis weight and size
, all there is the technical problem that speed is slow, accuracy rate is low in sorting.Annual kind of China ovum demand is very big, it is necessary to kind of pupa carry out high speed,
High-precision male and female sort, and the prior art cannot be satisfied such requirement.It is female due to utilizing genetic technique and gene editing technology
Property silkworm chrysalis can express fluorescent orange in whole life cycle, therefore using this characteristic help to solve above-mentioned technology to ask
Topic.For the silkworm chrysalis Sex judging technical problem underlying that silkworm egg manufacturing enterprise faces, cultivation Sex judging is needed to be easy, silkworm egg life
Efficient Silkworm varieties are produced, silkworm egg production cost is reduced, improves quality of silkworm eggs and enterprise's productivity effect.Therefore, how house is utilized
Silkworm male and female individuality genome at difference, establish quick, efficient, general Sex judging system be people pursue mesh
Mark.
Invention content
In view of this, the purpose of the present invention is to provide a kind of full-automatic male and female silkworm chrysalis sortings efficient, accuracy rate is high
Device, to solve of the existing technology to expend the low technical problem of a large amount of labours, accuracy rate.
Technical solution of the invention is to provide a kind of full-automatic male and female silkworm chrysalis sorting unit with lower structure, described
Female silkworm chrysalis in male and female silkworm chrysalis carries fluorescence protein gene, the full-automatic male and female silkworm chrysalis point after gene editing technical finesse
Screening device includes：
Feed mechanism：Silkworm chrysalis to be sorted is set to fall one by one on the corresponding station of transport mechanism；
Transport mechanism：Multiple stations are set, accepted when for feed mechanism blanking described in silkworm chrysalis to be sorted, and waited for point to described
Silkworm chrysalis is selected to be transmitted；
Detection module：In darkroom, the darkroom is in the transmission path of the transport mechanism, in the transport mechanism
By fall into station when sorting silkworm chrysalis and being transmitted in darkroom one by one, treat sorting silkworm chrysalis positioned at dark indoor detection module and carry out
The detection of male and female identifies, to confirm the gender of silkworm chrysalis to be sorted；
Blanking mechanism：After silkworm chrysalis sex-screening being completed in the transport mechanism by detection module, the transport mechanism
On silkworm chrysalis to be sorted fall within the blanking mechanism one by one；
Sorting mechanism：According to the detection module to the sex-screening recognition result of the silkworm chrysalis to be sorted, to silkworm chrysalis into
Row sorting so that the female pupa fallen from blanking mechanism enters female pupa recycling bins, and male pupa enters male pupa recycling bins.
Optionally, the feed mechanism includes hopper and feeding conveyer belt, and silkworm chrysalis to be sorted is put into hopper, silkworm chrysalis from
It is fallen at the lower opening of hopper in feeding conveyer belt, silkworm chrysalis is brought into and is undertaken in feeding conveyer belt by the feeding conveyer belt action
The charging conduit for conveying end, by the speed control blanking speed for adjusting feeding conveyer belt.
Optionally, the adjusting hairbrush worked as a buffer is equipped in the charging conduit.
Optionally, the transport mechanism includes chain and contains pupa box, and the chain is obliquely installed, and lower end is fallen close to described
Hopper, be uniformly arranged on chain contain pupa box, as station, fall from charging conduit described in silkworm chrysalis to be sorted fallen one by one in institute
It states and contains on pupa box.
Optionally, the detection module includes camera, light source, orange optical filter and green color filter, the light source
Green light is irradiated in silkworm chrysalis to be sorted through the green color filter, the female silkworm chrysalis with fluorescin is presented orange at this time, is taken the photograph
As head recognizes female silkworm chrysalis by orange optical filter, it is female that orange silkworm chrysalis, which is presented, and it is male that orange silkworm chrysalis is not presented,
Recognition result is sent to sorting mechanism, sorting recycling is carried out by sorting mechanism.
Optionally, the sorting mechanism is equipped with two slopes corresponding with female pupa recycling bins, male pupa recycling bins respectively,
When testing result is characterized as female pupa, then sorting mechanism is moved toward male pupa recycling bins so that the female pupa fallen from blanking mechanism is fallen
On the slope of female pupa recycling bins side, and falls into female pupa recycling bins along slope and then sorted when testing result is characterized as male pupa
Mechanism is moved toward female pupa recycling bins so that and the male pupa fallen from blanking mechanism is fallen on the slope in male pupa recycling bins side, and along
Slope falls into male pupa recycling bins.
Optionally, the camera is CCD camera, which is provided with light source, in fluoroscopic examination, silkworm chrysalis is by wavelength
After the light irradiation of 554NM, the internal bioluminescence substance of female can send out the transmitting light that wavelength is 586NM, and the camera is logical
It crosses load centre wavelength and captures bioluminescence for the cut-off fluorescent optical filter of the small bandwidth of 586NM.The light path of mechanism is set
It is calculated as:Use CREE CXA2530 high-powered LED lamps load 554NM narrow-cuts fluorescent optical filter for light source, the narrowband of 554NM
Optical filter ensures that the pure property of light source, the camera are filtered by loading the cut-off fluorescence for the small bandwidth that centre wavelength is 586NM
Mating plate captures bioluminescence.
Optionally, with CCD camera acquisition conveyer belt on silkworm chrysalis image, then by arithmetic programming to image at
Reason, judge the silkworm chrysalis male and female, then tables of data is established with MYSQL, finally utilize microcontroller control sorting mechanism to male and female silkworm chrysalis into
Row sorting.
Using structure of the invention, compared with prior art, has the following advantages：It is glimmering due to being implanted into biology in female silkworm chrysalis
Bioluminescence protein techniques are combined with machine vision technique, increase on camera and light source specific by photoprotein, the present invention
The optical filter of wavelength excludes the interference of non-excitation wavelength light, realizes that accurate, the high speed of male and female silkworm chrysalis identify.The present invention has
Advantage efficient, accuracy rate is high.
Description of the drawings
Fig. 1 is the structural schematic diagram of the full-automatic male and female silkworm chrysalis sorting unit of the present invention；
As shown in the figure, 1- hoppers, 2-1- feeding conveyer belts, 2-2- blanking mechanisms, 3- charging conduits, 4- adjust hairbrush, 5-1-
Sprocket wheel, 5-2- chains, 5-3- contain pupa box, 6-1- cameras, 6-2- light sources, the orange optical filters of 7-1,7-2- green color filters, 8-
Sorting mechanism, the female pupa recycling bins of 9-1-, 9-2- hero pupa recycling bins, 10- silkworm chrysalises.
Specific implementation mode
The preferred embodiment of the present invention is described in detail below in conjunction with attached drawing, but the present invention is not restricted to these
Embodiment.The present invention covers any replacement made in the spirit and scope of the present invention, modification, equivalent method and scheme.
In order to make the public have thorough understanding to the present invention, it is described in detail in following present invention preferred embodiment specific
Details, and description without these details can also understand the present invention completely for a person skilled in the art.
The present invention is more specifically described by way of example with reference to attached drawing in the following passage.It should be noted that attached drawing is adopted
Use with more simplified form and non-accurate ratio, only to it is convenient, lucidly aid in illustrating the embodiment of the present invention
Purpose.
Refering to what is shown in Fig. 1, the structure of full-automatic male and female silkworm chrysalis sorting unit of the invention is illustrated, in the male and female silkworm chrysalis
Female silkworm chrysalis carries fluorescence protein gene after gene editing technical finesse, and the full-automatic male and female silkworm chrysalis sorting unit includes：
Feed mechanism：Silkworm chrysalis 10 to be sorted is set to fall one by one on the corresponding station of transport mechanism；
Transport mechanism：Multiple stations are set, accepted when for feed mechanism blanking described in silkworm chrysalis to be sorted, and waited for point to described
Silkworm chrysalis is selected to be transmitted；
Detection module：In darkroom, the darkroom is in the transmission path of the transport mechanism, in the transport mechanism
By fall into station when sorting silkworm chrysalis and being transmitted in darkroom one by one, treat sorting silkworm chrysalis positioned at dark indoor detection module and carry out
The detection of male and female identifies, to confirm the gender of silkworm chrysalis to be sorted；
Blanking mechanism 2-2：After silkworm chrysalis sex-screening being completed in the transport mechanism by detection module, the conveyer
Silkworm chrysalis to be sorted on structure falls within the blanking mechanism one by one；
Sorting mechanism 8：According to the detection module to the sex-screening recognition result of the silkworm chrysalis to be sorted, to silkworm chrysalis into
Row sorting so that the female pupa fallen from blanking mechanism enters female pupa recycling bins 9-1, and male pupa enters male pupa recycling bins 9-2.
The feed mechanism includes hopper 1 and feeding conveyer belt 2-1, and silkworm chrysalis 10 to be sorted is put into hopper 1, silkworm chrysalis 10
It is fallen from the lower opening of hopper 1 in feeding conveyer belt 2-1, silkworm chrysalis is brought into and is undertaken in by the feeding conveyer belt 2-1 actions
The charging conduit 3 for expecting conveyer belt 2-1 conveyings end, by the speed control blanking speed for adjusting feeding conveyer belt 2-1.
The adjusting hairbrush 4 worked as a buffer is equipped in the charging conduit 3.
The transport mechanism includes that chain 5-2 is obliquely installed with Sheng pupa box 5-3, the chain 5-2, and lower end is close to described
Charging conduit 3, be uniformly arranged on chain 5-2 contain pupa box 5-3, as station, fall from charging conduit 3 described in silkworm chrysalis to be sorted
It falls one by one on the Sheng pupa box 5-3.Being obliquely installed can prevent multiple silkworm chrysalises from entering a Sheng pupa box 5-3, can be because tilting
And toward falling, hence into next Sheng pupa box 5-3.
The detection module includes camera 6-1, light source 6-2, orange optical filter 7-1 and green color filter 7-2, described
Green light is irradiated in silkworm chrysalis to be sorted by light source 6-2 through the green color filter 7-2, and the female silkworm chrysalis with fluorescin is at this time
Existing orange, camera 6-1 recognizes female silkworm chrysalis by orange optical filter 7-1, and it is female that orange silkworm chrysalis, which is presented, and orange is not presented
The silkworm chrysalis of color is male, and recognition result is sent to sorting mechanism, sorting recycling is carried out by sorting mechanism.
The camera is the CCD camera with fluorescence detection function, and the CCD camera passes through machine vision pair
Silkworm chrysalis is identified, and it is female that orange silkworm chrysalis, which is presented, and it is male that orange silkworm chrysalis is not presented, and recognition result is sent to sorting
Mechanism carries out sorting recycling by sorting mechanism.
Female silkworm chrysalis can express fluorescent orange in whole life cycle.The light that light source is sent out filters by green-emitting fluorescent
After piece, 554nm, the green light of bandwidth 30nm are generated, green light is passed through spectroscope, is irradiated on silkworm chrysalis, silkworm chrysalis is in green light
Under irradiation, if female silkworm chrysalis, then the fluorescent orange of 586nm can be inspired.Wavelength 586nm centered on fluorescent orange optical filter,
The optical filter of bandwidth 20nm can exclude the interference of other wavelength lights, can only be 586nm by wavelength, bandwidth 20nm's is orange
Light.Fluoroscopic examination camera determines that silkworm chrysalis is female silkworm chrysalis, is otherwise male silkworm chrysalis by the orange light wave of identification 586mn.Cause
The high speed identification to male and female silkworm chrysalis may be implemented by the combination of the devices such as Machine Vision Recognition Technology and illumination optical filtering in this.
The image of silkworm chrysalis in transport mechanism is acquired with CCD camera, then image is handled by arithmetic programming, is judged
The silkworm chrysalis male and female, then tables of data is established with MYSQL, it realizes the positioning to silkworm chrysalis, finally microcontroller is utilized to control sorting mechanism pair
Male and female silkworm chrysalis is sorted.
The specific course of work of the present invention is as follows：Silkworm chrysalis to be sorted is put into hopper 1, feeding conveyer belt 2-1 actions will
Silkworm chrysalis brings charging conduit 3 into, and feeding conveyer belt 2-1 can control blanking speed.It is provided in charging conduit 3 and adjusts hairbrush 4, hairbrush 4
Effect there are two, first, blanking speed can be controlled, two can be prevented from it is injured when silkworm chrysalis falls.Silkworm chrysalis itself is very soft, holds
It is vulnerable to machinery, physical injury, soft hairbrush can play buffering left and right, prevent silkworm chrysalis injured.It is provided on chain 5-2
Pupa box 5-3 is contained, when Sheng pupa tape, which silkworm chrysalis, reaches detection zone, camera is taken pictures, and male and female are told, and is then fallen in blanking mechanism
On 2-2, when detection device is determined as female pupa, sorting mechanism 8 moves downward, and female pupa falls into female pupa recycling bins 9-1；When detection fills
It sets when being determined as male silkworm chrysalis, sorting mechanism 8 moves right, and male pupa falls into male pupa recycling bins 9-2.It is arranged blanking mechanism 2-2's
Purpose is, prepares to sort, and to the time of sorting mechanism reaction, while playing the role of undertaking, can allow and know
The silkworm chrysalis not gone out is sorted with better posture.
It contains pupa box to be conveniently fixed on chain by screw, contains pupa box and formed by 3D printing according to the size of silkworm chrysalis, needle
To different silkworm chrysalis kinds, Sheng pupa box is of different sizes, contains pupa box interior smooth, prevents damage silkworm chrysalis.One can only be held by containing pupa box
Silkworm chrysalis.
Although embodiment is separately illustrated and is illustrated above, it is related to the common technology in part, in ordinary skill
Personnel apparently, can be replaced and integrate between the embodiments, be related to one of embodiment and the content recorded is not known, then
It can refer to another embodiment on the books.
Embodiments described above does not constitute the restriction to the technical solution protection domain.It is any in above-mentioned implementation
Modifications, equivalent substitutions and improvements etc., should be included in the protection model of the technical solution made by within the spirit and principle of mode
Within enclosing.
Claims (8)
1. a kind of full-automatic male and female silkworm chrysalis sorting unit, the female silkworm chrysalis in the male and female silkworm chrysalis is after gene editing technical finesse
With fluorescence protein gene, the full-automatic male and female silkworm chrysalis sorting unit includes：
Feed mechanism：Silkworm chrysalis to be sorted is set to fall one by one on the corresponding station of transport mechanism；
Transport mechanism：Multiple stations are set, silkworm chrysalis to be sorted described in undertaking when for feed mechanism blanking, and to the silkworm to be sorted
Pupa is transmitted；
Detection module：In darkroom, the darkroom will be fallen in the transmission path of the transport mechanism in the transport mechanism
Enter station when sorting silkworm chrysalis and being transmitted in darkroom one by one, treat sorting silkworm chrysalis positioned at dark indoor detection module and carry out male and female
Detection identification, to confirm the gender of silkworm chrysalis to be sorted；
Blanking mechanism：After completing silkworm chrysalis sex-screening by detection module in the transport mechanism, in the transport mechanism
Silkworm chrysalis to be sorted falls within the blanking mechanism one by one；
Sorting mechanism：According to the detection module to the sex-screening recognition result of the silkworm chrysalis to be sorted, silkworm chrysalis is divided
Choosing so that the female pupa fallen from blanking mechanism enters female pupa recycling bins, and male pupa enters male pupa recycling bins.
2. full-automatic male and female silkworm chrysalis sorting unit according to claim 1, it is characterised in that：The feed mechanism includes
Silkworm chrysalis to be sorted (10) is put into hopper (1), lower section of the silkworm chrysalis (10) from hopper (1) by hopper (1) and feeding conveyer belt (2-1)
Opening is fallen at feeding conveyer belt (2-1), and silkworm chrysalis is brought into and is undertaken in feeding conveyer belt by feeding conveyer belt (2-1) action
(2-1) conveys the charging conduit (3) of end, by the speed control blanking speed for adjusting feeding conveyer belt (2-1).
3. full-automatic male and female silkworm chrysalis sorting unit according to claim 2, it is characterised in that：It is set in the charging conduit (3)
There is the adjusting hairbrush (4) worked as a buffer.
4. full-automatic male and female silkworm chrysalis sorting unit according to claim 1, it is characterised in that：The transport mechanism includes chain
Item (5-2) and Sheng pupa box (5-3), the chain (5-2) are obliquely installed, and lower end is close to the charging conduit (3), in chain (5-
2) it is uniformly arranged on and contains pupa box (5-3), as station, fallen one by one in institute from the silkworm chrysalis to be sorted to fall in charging conduit (3)
It states and contains on pupa box (5-3).
5. full-automatic male and female silkworm chrysalis sorting unit according to claim 3, it is characterised in that：The detection module includes
Camera (6-1), light source (6-2), orange optical filter (7-1) and green color filter (7-2), described in the light source (6-2) penetrates
Green light is irradiated in silkworm chrysalis to be sorted by green color filter (7-2), and orange, camera is presented in the female silkworm chrysalis with fluorescin at this time
(6-1) recognizes female silkworm chrysalis by orange optical filter (7-1), and it is female that orange silkworm chrysalis, which is presented, and orange silkworm chrysalis is not presented
For male, recognition result is sent to sorting mechanism, sorting recycling is carried out by sorting mechanism.
6. full-automatic male and female silkworm chrysalis sorting unit according to claim 1, it is characterised in that：On the sorting mechanism (8)
It is then sorted equipped with two slopes corresponding with female pupa recycling bins, male pupa recycling bins respectively when testing result is characterized as female pupa
Mechanism is moved toward male pupa recycling bins so that and the female pupa fallen from blanking mechanism (2-2) is fallen on the slope in female pupa recycling bins side, and
Female pupa recycling bins are fallen into along slope, when testing result is characterized as male pupa, then sorting mechanism is moved toward female pupa recycling bins so that
The male pupa fallen from blanking mechanism (2-2) falls on the slope in male pupa recycling bins side, and falls into male pupa recycling bins along slope.
7. full-automatic male and female silkworm chrysalis sorting unit according to claim 5, it is characterised in that：On the camera (6-1)
Equipped with light source, in fluoroscopic examination, after silkworm chrysalis is irradiated by the light that wavelength is 554NM, the internal bioluminescence substance of female can be sent out
Go out the transmitting light that wavelength is 586NM, the camera filters by loading the cut-off fluorescence for the small bandwidth that centre wavelength is 586NM
Piece captures bioluminescence.
8. full-automatic male and female silkworm chrysalis sorting unit according to claim 7, it is characterised in that：It is acquired and is passed with CCD camera
The image for taking silkworm chrysalis is sent, then image is handled by arithmetic programming, judges the silkworm chrysalis male and female, then number is established with MYSQL
According to table, finally male and female silkworm chrysalis is sorted using microcontroller control sorting mechanism.
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| CN113634515A (en) * | 2021-09-24 | 2021-11-12 | 桂林航天工业学院 | Full-automatic male and female silkworm chrysalis sorting equipment |
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| EP3996500A4 (en) * | 2019-07-11 | 2023-07-05 | Verily Life Sciences LLC | Pupae singulator and sex sorter |
| US11440057B2 (en) | 2019-07-11 | 2022-09-13 | Verily Life Sciences Llc | Pupae singulator and sex sorter |
| CN110575965B (en) * | 2019-09-30 | 2021-06-01 | 中国计量大学 | Silkworm pupa male and female screening method for near infrared spectrum identification |
| EP4275487A1 (en) * | 2022-05-09 | 2023-11-15 | Bühler AG | Insect sorting device for the sorting of live insects, handling unit for handling insects comprising an insect sorting device and farm for rearing insects |
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