CN108504765B - 实时荧光pcr真菌检测引物、探针、试剂盒及检测方法 - Google Patents
实时荧光pcr真菌检测引物、探针、试剂盒及检测方法 Download PDFInfo
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- CN108504765B CN108504765B CN201810536632.8A CN201810536632A CN108504765B CN 108504765 B CN108504765 B CN 108504765B CN 201810536632 A CN201810536632 A CN 201810536632A CN 108504765 B CN108504765 B CN 108504765B
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- probe
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
名称 | 量值(ul) |
10×PCR buffer | 2.5 |
5×probe buffer | 5 |
20mM dN(U)TP | 0.2 |
5U/ul Hotstart Taq | 0.2 |
2U/ul UDG | 0.1 |
10uM ITSF1 | 1 |
10uM ITSR1 | 0.1 |
10uM ICF1 | 0.1 |
10uM ICR1 | 0.1 |
10uM Probe | 各0.5 |
DNA | 4 |
Total | 25 |
Claims (5)
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CN201810536632.8A CN108504765B (zh) | 2018-05-30 | 2018-05-30 | 实时荧光pcr真菌检测引物、探针、试剂盒及检测方法 |
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CN201810536632.8A CN108504765B (zh) | 2018-05-30 | 2018-05-30 | 实时荧光pcr真菌检测引物、探针、试剂盒及检测方法 |
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CN108504765A CN108504765A (zh) | 2018-09-07 |
CN108504765B true CN108504765B (zh) | 2022-04-01 |
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113403421A (zh) * | 2021-08-05 | 2021-09-17 | 丹娜(天津)生物科技股份有限公司 | 一种毛霉菌分型检测的引物探针组合及其应用 |
CN115011714B (zh) * | 2022-04-06 | 2024-06-04 | 四川众志禾生物技术有限责任公司 | 检测真菌的引物组、探针组、试剂盒、用途及检测方法 |
CN117051168A (zh) * | 2023-10-11 | 2023-11-14 | 北京量觉科技有限责任公司 | 一种检测隐球菌的引物探针组合、方法以及试剂盒 |
CN117512204B (zh) * | 2024-01-05 | 2024-04-09 | 江苏美克医学技术有限公司 | 曲霉菌属、新型隐球菌、耶氏肺孢子多重检测的引物和探针的组合、试剂盒及应用 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6180339B1 (en) * | 1995-01-13 | 2001-01-30 | Bayer Corporation | Nucleic acid probes for the detection and identification of fungi |
CN1286989C (zh) * | 2005-04-22 | 2006-11-29 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | 检测致病真菌生物芯片 |
PT103277B (pt) * | 2005-05-17 | 2007-03-30 | Univ Do Minho | Fragmentos de dna e primers para a detecção e identificação de espécies de cândida clinicamente relevantes |
CN108034745B (zh) * | 2017-12-21 | 2020-04-17 | 杭州缔蓝生物技术有限公司 | 一种同时检测四种念珠菌的引物探针组合和试剂盒 |
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Address after: 311200 room 911, block C, no.198, Qidi Road, economic and Technological Development Zone, Xiaoshan District, Hangzhou City, Zhejiang Province Patentee after: HANGZHOU QIANJI BIOTECHNOLOGY Co.,Ltd. Patentee after: Hangzhou Meilian Medical Co.,Ltd. Address before: 311200 room 911, block C, no.198, Qidi Road, economic and Technological Development Zone, Xiaoshan District, Hangzhou City, Zhejiang Province Patentee before: HANGZHOU QIANJI BIOTECHNOLOGY Co.,Ltd. Patentee before: HANGZHOU MEILIAN MEDICAL EXAMINATION INSTITUTE Co.,Ltd. |
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