CN108498710A - A kind of preparation method of stem of noble dendrobium freeze-dried powder - Google Patents
A kind of preparation method of stem of noble dendrobium freeze-dried powder Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
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- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/31—Extraction of the material involving untreated material, e.g. fruit juice or sap obtained from fresh plants
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention discloses a kind of preparation methods of stem of noble dendrobium freeze-dried powder, overall process does not have high temperature, the factors such as be exposed to the sun influence, the bioactivity and Clinical efficacy of stem of noble dendrobium active ingredient are remained to the maximum extent, it obtains after testing, using the stem of noble dendrobium freeze-dried powder active ingredient of lyophilization than drying, the stem of noble dendrobium of high temperature drying is higher by 15% or so, and bioactivity higher, clinical efficacy is more preferable, and the stem of noble dendrobium is homogenized after Ultrasonic Pulverization, stem of noble dendrobium freeze-dried powder smashing fineness obtained greatly promotes, nanoscale can be reached, it is directly oral, it is easier to be digested, drug effect can preferably be played.
Description
Technical field
The present invention relates to a kind of stem of noble dendrobium freeze-dried powder, the preparation method of specifically a kind of stem of noble dendrobium freeze-dried powder.
Background technology
Dendrobium candidum (scientific name：DendrobiumofficinaleKimuraetMigo), also known as：Ribbed hedyotis herb, Yunnan iron sheet,
Iron sheet bucket maple.Belong to micro- specific item, the perennial herbaceous plant that grows nonparasitically upon another plant of orchid family.Stem is upright, cylindrical, 9-35 centimetres long, 2-4 millimeters thick,
Sepal and petal yellow green, close similar, long round shape lanceolar is about 1.8cm, wide 4~5mm, 3~June of florescence.It is distributed mainly on
The ground such as Chinese Anhui, Zhejiang, Fujian.Its stem is used as medicine, and belongs to the yin tonics in tonic.
Dendrobium candidum is herbaceos perennial, is distributed widely in the ground such as Guangxi, Yunnan, Zhejiang, Fujian, is typically distributed about
On the dark and damp rock in mountainous region half.
The morphological feature of dendrobium candidum：Stem is upright, cylindrical, 9-35 centimetres long, 2-4 millimeters thick, not branch, has more piece,
1-3-1.7 centimetres of internode length, often in middle part 3-5 pieces of leaf of the above alternate；Ye Erlie, papery, long round shape lanceolar, long 3-4 (- 7)
Centimetre, wide 9-11 (- 15) millimeter, apex is blunt and how many hook turn, and base portion is downward to embrace the sheath of stem, and edge and middle rib are often with pale purple
Color；Leaf sheath often with purple plague purpura, when old its upper limb and stem pine from and opens, and leave 1 ashen gap of ring-type with saving.
Raceme is often sent out from the old stem top for having fallen leaf, has 2-3 flower；Common peduncle is 5-10 millimeters long, and base portion has 2-3
Piece short sheath；Rachis inflection sigmoid, it is 2-4 centimetres long；Petal piece dry film matter, shallow white is oval, 5-7 millimeters long, and apex is slightly
It is blunt；Bennet and ovary are 2-2.5 centimetres long；Sepal and petal yellow green, nearly similar, long round shape lanceolar is about 1.8 centimetres, wide
4-5 millimeters, the sharp point of apex has 5 arteries and veins；Side sepal base portion is broader, about 1 centimetre wide；Calyx capsule cone, is about 5 millimeters, end
It is round；Lip white, base portion have the corpus callosum of 1 green or yellow, and ovum shape lanceolar is more slightly shorter than sepal, middle part reflexed, apex
Anxious point, does not split or unobvious 3 is split, and both sides below middle part have aubergine striped, and how much wavy edge is；Lip disk gathers thin papillary
Hair, and have 1 aubergine patch more than middle part；Stamen column yellow green is about 3 millimeters, and apex both sides respectively have 1 purple point；Stamen
Column foot yellow green band aubergine striped, thinly covered hair；Anther cap white, long ovate triangle are about 2.3 millimeters, and the nearly sharp point in top is simultaneously
And 2 split.The month at florescence 3-6.
In terms of habit, dendrobium candidum is suitable in nice and cool, moistening, the ambient growth of clear air.Usually it is born in sea
It pulls out up on the dark and damp rock in 1600 meters of mountainous region half, the environment of happiness warm and moist weather and half cloudy half light can not resist cold.
In terms of geographical distribution, dendrobium candidum is distributed widely in the Anhui west and south (Dabie Mountain), Eastern Part of Zhejiang Province, China (Yinxian County, day
Platform, fairy house), In Western Fujian Province (Ninghua), the Guangxi northwestward (Tiane), Sichuan, southeastern Yunnan (stone screen, mountain of papers, Malipo, west
Farmland).
In terms of propagation method, fast numerous method and plant division cutting propagation etc. in a organized way.
Tissue rapid propagation method：14~21 days hardenings should be first carried out before tissue-cultured seedling cultivation, concrete measure is to move to tissue-cultured seedling
Hardening room makes its gradual reform of nature environment in the environment of open variation, can bottle outlet plantation when tissue-cultured seedling leaf color is dark green.
First bottle cap is opened before bottle outlet, bottle seedling is allowed to be placed in outdoor air 2~3 days, allows its reform of nature humiture.So
After carry out washing seedling, the seedling with culture medium is gently taken out when washing seedling, is placed in basin and cleans, pollutes seedling and bare-root seeding or few root
Seedling is answered separated.Tissue-cultured seedling is first cleaned with tap water, should mainly wash off agar, in case agar is mouldy to cause rotten, then
It is rinsed with clear water again.When washing seedling preferably based on the size of seedling and quality synchronize be classified, to cultivate, to manage, improve
The survival rate of tissue-cultured seedling simultaneously keeps growth neat.
Plant division cutting propagation method：Selected when plant division life in 1 year or life in 2 years, color and luster it is light green, it is healthy and strong, sprout more, well developed root system and
The plant of no disease and pests harm makees kind of a strain, cuts off deadwood, disconnected branch, old branch and long fibrous root, strain clump is cut, and is divided into little Cong, every clump
Stem strain with leaf 5~7, you can plantation.
In terms of cultivation technique, the planting time of dendrobium candidum is usually annual spring and autumn, and spring is better than autumn.
In Zhejiang area, the Best Times of officinal dendrobium stem plantation are annual mid-April to late June, and this period, temperature was 12~25
DEG C, and air humidity is larger, the test tube seedling transplanting survival rate after bottle outlet is higher and growth time is longer；Followed by mid-September is extremely
Late October, the transplanting of this period are important to carry out the antifreeze work of cold-resistant.The cultivation matrix of dendrobium candidum is High efficiency and high quality cultivation
The biological nature of key, dendrobium candidum requires the existing good water-retaining property of cultivation matrix to have ventilating air permeability, large-scale production again
It is required that cultivation matrix raw material is easy to get, is easy to operate.Report mesostroma has sphagna, rubble, peanut shell, moss, coconut skin, pine tree
Skin, sawdust, charcoal, wooden unit etc., application in production is mainly bark, sawdust or bark, sawdust, rubble, organic fertilizer mixing
Object, wherein bark are ground into 2~3cm or less particles.Officinal dendrobium stem plantation requires to carry out in greenhouse, and the construction of greenhouse requires to do
It keeps off the rain to ventilation, shade, have fly net, and according to the habit of dendrobium candidum, consider the illumination in place, temperature, humidity, lead to
The natural causes such as wind.The preference temperature of Growth of Dendrobium candidum is 15~30 DEG C, and in Zhejiang area, summer is more hot, general to use
80% sunshade net covering, is raised plastic film, is cooled down with profit；Winter is colder, with double under 30%~50% sunshade degree
Layer plastic membrane sealing heat preservation.Dendrobium candidum requires that matrix moistening, air humidity is kept to keep 80% or more preferably, but cannot
Ponding, it is best using sprinkling irrigation or trickle irrigation when watering, it must not irrigation.Different seasons it is different regional irrigation amount it is also different.
Version in 2010《Chinese Pharmacopoeia》The dendrobium candidums of page 265.Dendrobium candidum is as orchid dendrobium candidum
The drying stem of DendrobiumofficinaleKimuraetMigo, plant dendrobium candidum are harvested March in November to next year, are removed
Impurity cuts off part fibrous root, is twisted into spiral shape or spring-like in heating, dries, here it is " Tiepi Fengdous " of people's common name
(earrings dendrobium)；Or cut into chunks, dry or low temperature drying is exactly " dendrobium candidum " of people's common name.As it can be seen that there are three among these
Appellation, one is fresh plant dendrobium candidum, i.e. orchid dendrobium candidum DendrobiumofficinaleKimuraetMigo；Its
Two be the fresh plant through dry and be twisted into the drying stem of spiral shape or spring-like, is known as " Tiepi Fengdou " medicinal material；The third is should
Fresh plant is through cutting into chunks and drying, " dendrobium candidum " medicinal material.Above-mentioned pharmacopeia records the big gun for specifying orchid dendrobium candidum
Method processed, and usually final products form of the people from commercially available orchid dendrobium candidum.As it can be seen that classical
Dendrobium candidum processing, application method are to need to dry fresh orchid dendrobium candidum.It is well known that large batch of iron
The problem of drying of the skin stem of noble dendrobium needs stringent control to go mouldy, and need in this drying process to use smoking process, it is therefore, existing
Process so that various forms of dendrobium candidums is generated secondary pollutions, such as the sulfur content in medicine materical crude slice can be caused seriously to surpass
Mark, so as to cause serious injury to health.
The dendrobium candidum product of existing market sale, mostly uses stem dry product, i.e. Tiepi Fengdou is made, it is by dendrobium candidum
After cutting off blade and fibrous root, spiral shape or spring-like dry product are twisted into when drying, process substantially need by arrangement, baking,
Curling plus hoop, dry 4 processing steps are related to baking and banking up with earth and drying inherently to some thermal sensitivity in entire processing link
Nutriment generates destruction, or reduces its content.Therefore, it is necessary to develop new stem of noble dendrobium fresh medicine processing technology, research and develop
Convenient to take, with obvious effects, the higher stem of noble dendrobium fresh medicine of nutritive value.
Invention content
The purpose of the present invention is to provide a kind of preparation methods of stem of noble dendrobium freeze-dried powder, to solve to propose in above-mentioned background technology
The problem of.
To achieve the above object, the present invention provides the following technical solutions：
A kind of preparation method of stem of noble dendrobium freeze-dried powder, includes the following steps：1) feedstock processing：Fresh stem of noble dendrobium complete stool is taken, is removed
Dead leaf corrupt tissue, cleaned standby seam；2) will treated that the fresh stem of noble dendrobium is added after pure water is squeezed into homogenate carries out boiling, will be digested
It is 100-110 mesh that the water cooking liquid generated in journey, which utilizes strainer filtering, strainer mesh number, then utilizes decanter centrifuge in 5000-
Carry out centrifugal treating under conditions of 5500 revs/min, cross ceramic membrane concentration, 0.2 to 0.5 μm of membrane aperture, before film pressure be 0.1 to
0.3MPa crosses NF membrane and concentrates, 0.01-0.05 μm of membrane aperture, and pressure is 0.1-0.3MPa before film；3) it will be handled through above-mentioned steps
Water cooking liquid afterwards is put into quick-frozen storehouse and carries out quick-frozen, -40 DEG C to -25 DEG C of quick freezing temperature, quick-frozen time 2-4h；4) solid-state will be frozen into
Material be put into freeze-drying storehouse, and to dryness storehouse vacuumize process, vacuum degree 10Pa opens heating spoke to 100Pa
Penetrate, heating radiation temperature is 80 DEG C to 90 DEG C, and keeps temperature of charge in 40 DEG C or less freeze-drying time 12-24h, 5) by material powder
It is broken into powdered.
As a further solution of the present invention：In the step 2), 0.4-1.5g emulsifiers are added in homogenate per 100Kg,
After mixing, homogenate is handled 5-10 minutes using ultrasonic cell disruption instrument.
As a further solution of the present invention：In the step 2), the solid-to-liquid ratio of homogenate and pure water is 1g：(100-120)
m。
As a further solution of the present invention：In the step 5), the mode of crushing is Ultrasonic Pulverization.
As further scheme of the invention：It further include step 6)：Powdered object merging freezing after super crushing is dry
In dry machine material disc, pre-freeze 1~2 hour, then freezed under conditions of vacuum degree is -15~-20Pa, temperature is -25~-35 DEG C
20~40 hours, make water sublimed to get to stem of noble dendrobium freeze-dried powder.
Compared with prior art, the beneficial effects of the invention are as follows：Overall process of the present invention such as does not have high temperature, is exposed to the sun at the factors shadow
It rings, remains the bioactivity and Clinical efficacy of stem of noble dendrobium active ingredient to the maximum extent, obtain after testing, using lyophilization
Stem of noble dendrobium freeze-dried powder active ingredient be higher by 15% or so than the stem of noble dendrobium of drying, high temperature drying, and bioactivity higher, clinical treat
Effect is more preferable, and stem of noble dendrobium homogenate, after Ultrasonic Pulverization, stem of noble dendrobium freeze-dried powder smashing fineness obtained greatly promotes, and can reach nanometer
Grade, it is directly oral, it is easier to be digested, can preferably play drug effect.
Specific implementation mode
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
The every other embodiment that art personnel are obtained without making creative work belongs to the model that the present invention protects
It encloses.
Embodiment 1：A kind of preparation method of stem of noble dendrobium freeze-dried powder, includes the following steps：1) feedstock processing：Take the fresh stem of noble dendrobium complete
Strain, removal dead leaf corrupt tissue, cleaned standby seam；2) will treated that the fresh stem of noble dendrobium is added after pure water is squeezed into homogenate carries out boiling,
It is 100 mesh that the water cooking liquid generated in digestion process, which is utilized strainer filtering, strainer mesh number, then utilizes decanter centrifuge 5000
Centrifugal treating is carried out under conditions of rev/min, crosses ceramic membrane concentration, 0.2 μm of membrane aperture, pressure is 0.1MPa before film, crosses nanofiltration
Film concentrates, 0.01 μm of membrane aperture, and pressure is 0.1MPa before film；3) will through above-mentioned steps treated water cooking liquid is put into quick-frozen storehouse into
Scanning frequency is frozen, -40 DEG C of quick freezing temperature, quick-frozen time 2h；4) solid material will be frozen into be put into freeze-drying storehouse, and to drying
Storehouse vacuumize process, vacuum degree 10Pa, while heating radiation is opened, heating radiation temperature is 80 DEG C and keeps temperature of charge 40
DEG C or less freeze-drying time 12h, 5) by crushing material at powdered.
In the step 2), 0.4g emulsifiers are added in homogenate per 100Kg, after mixing, homogenate are utilized into ultrasonic wave
Cell crushing instrument is handled 5 minutes.
In the step 2), the solid-to-liquid ratio of homogenate and pure water is 1g：100m.
In the step 5), the mode of crushing is Ultrasonic Pulverization.
It further include step 6)：In powdered object merging freeze drier material disc after super crushing, pre-freeze 1 hour, then
It is freezed 20 hours under conditions of vacuum degree is -15Pa, temperature is -25 DEG C, makes water sublimed to get to stem of noble dendrobium freeze-dried powder.
Embodiment 2：A kind of preparation method of stem of noble dendrobium freeze-dried powder, includes the following steps：1) feedstock processing：Take the fresh stem of noble dendrobium complete
Strain, removal dead leaf corrupt tissue, cleaned standby seam；2) will treated that the fresh stem of noble dendrobium is added after pure water is squeezed into homogenate carries out boiling,
It is 110 mesh that the water cooking liquid generated in digestion process, which is utilized strainer filtering, strainer mesh number, then utilizes decanter centrifuge 5500
Centrifugal treating is carried out under conditions of rev/min, crosses ceramic membrane concentration, 0.5 μm of membrane aperture, pressure is 0.3MPa before film, crosses nanofiltration
Film concentrates, 0.05 μm of membrane aperture, and pressure is 0.3MPa before film；3) will through above-mentioned steps treated water cooking liquid is put into quick-frozen storehouse into
Scanning frequency is frozen, -25 DEG C of quick freezing temperature, quick-frozen time 4h；4) solid material will be frozen into be put into freeze-drying storehouse, and to drying
Storehouse vacuumize process, vacuum degree 100Pa, while heating radiation is opened, heating radiation temperature is 90 DEG C, and temperature of charge is kept to exist
40 DEG C or less freeze-drying times for 24 hours, 5) by crushing material at powdered.
In the step 2), 1.5g emulsifiers are added in homogenate per 100Kg, after mixing, homogenate are utilized into ultrasonic wave
Cell crushing instrument is handled 10 minutes.
In the step 2), the solid-to-liquid ratio of homogenate and pure water is 1g：120m.
In the step 5), the mode of crushing is Ultrasonic Pulverization.
It further include step 6)：In powdered object merging freeze drier material disc after super crushing, pre-freeze 2 hours, then
It is freezed 40 hours under conditions of vacuum degree is -20Pa, temperature is -35 DEG C, makes water sublimed to get to stem of noble dendrobium freeze-dried powder.
Embodiment 3：A kind of preparation method of stem of noble dendrobium freeze-dried powder, includes the following steps：1) feedstock processing：Take the fresh stem of noble dendrobium complete
Strain, removal dead leaf corrupt tissue, cleaned standby seam；2) will treated that the fresh stem of noble dendrobium is added after pure water is squeezed into homogenate carries out boiling,
It is 105 mesh that the water cooking liquid generated in digestion process, which is utilized strainer filtering, strainer mesh number, then utilizes decanter centrifuge 5300
Centrifugal treating is carried out under conditions of rev/min, crosses ceramic membrane concentration, 0.3 μm of membrane aperture, pressure is 0.2MPa before film, crosses nanofiltration
Film concentrates, 0.03 μm of membrane aperture, and pressure is 0.2MPa before film；3) will through above-mentioned steps treated water cooking liquid is put into quick-frozen storehouse into
Scanning frequency is frozen, -30 DEG C of quick freezing temperature, quick-frozen time 3h；4) solid material will be frozen into be put into freeze-drying storehouse, and to drying
Storehouse vacuumize process, vacuum degree 50Pa, while heating radiation is opened, heating radiation temperature is 80 DEG C, and temperature of charge is kept to exist
40 DEG C or less freeze-drying time 18h, 5) by crushing material at powdered.
In the step 2), 1.0g emulsifiers are added in homogenate per 100Kg, after mixing, homogenate are utilized into ultrasonic wave
Cell crushing instrument is handled 8 minutes.
In the step 2), the solid-to-liquid ratio of homogenate and pure water is 1g：110m.
In the step 5), the mode of crushing is Ultrasonic Pulverization.
It further include step 6)：In powdered object merging freeze drier material disc after super crushing, pre-freeze 1 hour, then
It is freezed 20 hours under conditions of vacuum degree is -15Pa, temperature is -25 DEG C, makes water sublimed to get to stem of noble dendrobium freeze-dried powder.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Profit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent requirements of the claims
Variation is included within the present invention.In addition, it should be understood that although this specification is described in terms of embodiments, but it is not each
Embodiment only contains an independent technical solution, and this description of the specification is merely for the sake of clarity, this field
Technical staff should consider the specification as a whole, and the technical solutions in the various embodiments may also be suitably combined, form this
The other embodiment that field technology personnel are appreciated that.
Claims (5)
1. a kind of preparation method of stem of noble dendrobium freeze-dried powder, which is characterized in that include the following steps：1) feedstock processing：Take the fresh stem of noble dendrobium
Complete stool, removal dead leaf corrupt tissue, cleaned standby seam；It 2) will treated that the fresh stem of noble dendrobium is added after pure water is squeezed into homogenate steams
It boils, it is 100-110 mesh that the water cooking liquid generated in digestion process, which is utilized strainer filtering, strainer mesh number, is then centrifuged using the spiral shell that crouches
Machine carries out centrifugal treating under conditions of 5000-5500 revs/min, crosses ceramic membrane and concentrates, 0.2 to 0.5 μm of membrane aperture, is pressed before film
Power is 0.1 to 0.3MPa, crosses NF membrane and concentrates, 0.01-0.05 μm of membrane aperture, and pressure is 0.1-0.3MPa before film；It 3) will be through upper
It states the water cooking liquid after step process and is put into quick-frozen, -40 DEG C to -25 DEG C of the quick freezing temperature of quick-frozen storehouse progress, quick-frozen time 2-4h；4) will
It has been frozen into solid material to be put into freeze-drying storehouse, and to dryness storehouse vacuumize process, vacuum degree 10Pa to 100Pa, simultaneously
Heating radiation is opened, heating radiation temperature is 80 DEG C to 90 DEG C, and keeps temperature of charge in 40 DEG C or less freeze-drying time 12-24h,
5) by crushing material at powdered.
2. the preparation method of stem of noble dendrobium freeze-dried powder according to claim 1, which is characterized in that in the step 2), per 100Kg
0.4-1.5g emulsifiers are added in homogenate, after mixing, homogenate is handled 5-10 minutes using ultrasonic cell disruption instrument.
3. the preparation method of stem of noble dendrobium freeze-dried powder according to claim 1, which is characterized in that in the step 2), homogenate with
The solid-to-liquid ratio of pure water is 1g：(100-120)m.
4. the preparation method of stem of noble dendrobium freeze-dried powder according to claim 1, which is characterized in that in the step 5), crushing
Mode is Ultrasonic Pulverization.
5. the preparation method of stem of noble dendrobium freeze-dried powder according to claim 1, which is characterized in that further include step 6)：It is super to crush
Afterwards powdered object merging freeze drier material disc in, pre-freeze 1~2 hour, then vacuum degree be -15~-20Pa, temperature
It is freezed 20~40 hours under conditions of being -25~-35 DEG C, makes water sublimed to get to stem of noble dendrobium freeze-dried powder.
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| CN106822605A (en) * | 2017-03-31 | 2017-06-13 | 泸州德高生物科技有限公司 | The preparation method of the ecological oral freeze-dried powder of the stem of noble dendrobium |
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2018
- 2018-05-22 CN CN201810492824.3A patent/CN108498710A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102687875A (en) * | 2012-04-25 | 2012-09-26 | 大连棒棰岛海产股份有限公司 | Preparation method of freeze-dried powder of sea cucumber boiled liquid |
| CN106822605A (en) * | 2017-03-31 | 2017-06-13 | 泸州德高生物科技有限公司 | The preparation method of the ecological oral freeze-dried powder of the stem of noble dendrobium |
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