CN108497246A - A kind of plant weight losing function beverage and preparation method thereof - Google Patents
A kind of plant weight losing function beverage and preparation method thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 235000013361 beverage Nutrition 0.000 title claims abstract description 24
- 239000000284 extract Substances 0.000 claims abstract description 80
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 45
- 244000037364 Cinnamomum aromaticum Species 0.000 claims abstract description 33
- 235000014489 Cinnamomum aromaticum Nutrition 0.000 claims abstract description 33
- 244000223014 Syzygium aromaticum Species 0.000 claims abstract description 33
- 235000016639 Syzygium aromaticum Nutrition 0.000 claims abstract description 32
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 29
- 240000000249 Morus alba Species 0.000 claims abstract description 29
- 230000000694 effects Effects 0.000 claims abstract description 22
- 241000196324 Embryophyta Species 0.000 claims abstract description 21
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 21
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 21
- 235000014360 Punica granatum Nutrition 0.000 claims abstract description 20
- 150000004056 anthraquinones Chemical class 0.000 claims abstract description 20
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 18
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N flavone Chemical compound O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims abstract description 17
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- 241000218213 Morus <angiosperm> Species 0.000 description 1
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- 150000004665 fatty acids Chemical class 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
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- 230000002218 hypoglycaemic effect Effects 0.000 description 1
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- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 1
- 235000008777 kaempferol Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
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- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 1
- 208000001797 obstructive sleep apnea Diseases 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 229920001190 pomegranate ellagitannin Polymers 0.000 description 1
- LMIBIMUSUFYFJN-RSVYENFWSA-N punicalagin Natural products O[C@@H]1O[C@@H]2COC(=O)c3cc(O)c(O)c(O)c3c4c(O)cc5OC(=O)c6c(c(O)c(O)c7OC(=O)c4c5c67)c8c(O)c(O)c(O)cc8C(=O)O[C@H]2[C@@H]9OC(=O)c%10cc(O)c(O)c(O)c%10c%11c(O)c(O)c(O)cc%11C(=O)O[C@@H]19 LMIBIMUSUFYFJN-RSVYENFWSA-N 0.000 description 1
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- 230000002195 synergetic effect Effects 0.000 description 1
- 229920002258 tannic acid Polymers 0.000 description 1
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 description 1
- 229940033123 tannic acid Drugs 0.000 description 1
- 235000015523 tannic acid Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of plant weight losing function beverage and preparation method thereof, the sum of weight percent is counted for 100%, and the weight percent of contained each ingredient is in the plant weight losing function beverage：EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 0.11~0.52%, cloves chromocor extract 0.30~0.68%, mulberry leaf polyphenol extract 0.18~0.62%, cassia seed anthraquinones extract 0.15~0.38%, sucrose 0.02~0.08%, Aspartame 0.01~0.05%, acesulfame potassium 0.01~0.05%, vitamin C 0.05~0.20%, citric acid 0.015~0.03%, remaining is purified water.Drinks green natural of the present invention, sweet mouthfeel have faint scent, and rich in plurality of active ingredients such as total polyphenols, general flavone, general anthraquinones, can play the health-care efficacy of pre- preventing obesity.
Description
Technical field
The invention belongs to technical field of health care food, and in particular to a kind of plant weight losing function beverage and preparation method thereof.
Background technology
1997, the World Health Organization（WHO）Obesity is clearly declared as a kind of disease.Obesity is one kind due to a variety of
Chronic metabolic diseases caused by factor are mainly shown as that the histiocytic quantity of body fat and volume increase, lead to body
The percentage of body fat weight percentage of liveweight increases extremely.It is that fatty liver, artery are athero- in addition, obesity can also lead to a variety of diseases
The direct pathogenic factor of the diseases such as hardening, Sleep Apnea-hypopnea Syndrome, hyperlipemia, high-cholesterol disease.In recent years,
No matter in developed country or in developing country, incidence of obesity is all in rising trend.WHO Report points out,
There are about 2.5 hundred million adults to suffer from obesity in the whole world at present, and the direct or indirect death toll that obesity is facilitated is every year up to 300,000 people.
Therefore, a urgent problem to be solved is become for fat treatment.Currently, there are many medicament categories for the treatment of obesity, mainly
Have two class of chemical drugs and autonomic drug, but treat the chemical drugs of obesity and autonomic drug have mostly certain side effect with it is bad
Reaction, such as steatorrhea are weak etc..Therefore, good, the safe slimming drug of development efficacy or health products have a vast market
Demand and great social effect.
Fatty acid sythetase inhibitor does not enter blood, not will produce as a kind of slimming medicine acted in enteron aisle
Central nervous system side effect, does not influence mineral metabolism, is the hot spot of Recent study.And each Plant Extracts are as fat
The source of acid enzyme inhibitor has very huge potentiality, and its safety higher, source are more extensive, will be novel
It occupies an important position in slimming drug or health products exploitation.
Granatum is fruit pomegranate（Punica granatumL.）Pericarp after drying.Chemical composition in granatum with
Based on tannin class, alkaloid, organic acid and flavonoids.Studies have shown that the punicalagins containing high level, tan flower in granatum
The polyphenols such as acid have very strong fatty acid synthetase inhibitory activity.Cloves is plant clove of myrtaceae（Syzygium aromaticum (L.) Merr. & L. M. Perry）Dry flower, main active has eugenol, eugenol
Ester, β-carypohyllene, tannic acid, gaultherolin, Kaempferol, rhamnocitrin, peach element pavilion and vanillic aldehyde etc..Research confirmation, fourth
Fragrant extract has fatty acid synthetase inhibitory activity.Mulberry leaf are mulberry（Morus albaL.）Blade, it is mild-natured, it is sweet in flavor, tool
There is the effect of dispelling wind and heat from the body, clearing lung-heat ravine is dry, clears liver and improve vision, is clinically to use more a herb.Modern chemistry studies table
It is bright, flavonoids, phenolic acid class, alkaloids and polysaccharide isoreactivity ingredient are contained in mulberry leaf.Polyphenol contained by mulberry leaf have it is hypoglycemic,
A variety of physiological activity such as reducing blood lipid, blood pressure lowering, antiviral, inhibition fatty acid synthetase.Cassia seed is that species Cassia platymiscium is determined
It is bright（Cassia toraLinn.）Dry mature seed.Cassia seed first recorded in《Sheng Nong's herbal classic》, it is classified as top grade.1993,
Cassia seed is classified as integration of drinking and medicinal herbs raw material by health ministry.
Invention content
The purpose of the present invention is to provide a kind of plant weight losing function beverage, the beverage is by granatum, cloves, mulberry leaf, certainly
The raw material of these three integration of drinking and medicinal herbs of pine torch is made, pure natural, is free of toxic and harmful substance, can be used for fat prevention and protects
It is strong.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of plant weight losing function beverage, the sum of weight percent are 100% meter, and the weight percent of contained each ingredient is：
EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 0.11~0.52%, cloves chromocor extract 0.30~0.68%, mulberry leaf polyphenol extract 0.18~
0.62%, cassia seed anthraquinones extract 0.15~0.38%, sucrose 0.02~0.08%, Aspartame 0.01~0.05%, acesulfame potassium
0.01~0.05%, vitamin C 0.05~0.20%, citric acid 0.015~0.03%, remaining is purified water.
The preparation method of the plant weight losing function beverage includes the following steps：
（1）The preparation of EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL：Pericarpium granati, chopping are collected, the low temperature drying at 40~60 DEG C crushes, mistake
200 mesh sieve；Then solid-liquid ratio 1 is pressed:15~1:The ethanol solution of mass concentration 60%, ultrasonic extraction 5~10 is added in 25 g/mL
Min, filtering, filter residue repeat extraction 1~2 time, merge extracting solution, obtain pericarpium granati extracting solution, be concentrated under reduced pressure into no alcohol taste, on
D101 macroporous adsorption resin chromatography columns remove macromolecular water soluble protein and polysaccharide with water elution, then dense with quality successively
The ethanol solution of degree 20%, 40%, 60%, 80%, 100% carries out gradient elution, and the ethyl alcohol for collecting 60%~80% position is washed
De- liquid, vacuum-concentrcted at 60 DEG C, obtains EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL；
（2）The preparation of cloves chromocor extract：Cloves bud is collected, is dried under the conditions of 40~60 DEG C, is crushed, 120 mesh sieve is crossed；So
Solid-liquid ratio 1 is pressed afterwards:15~1:The ethanol solution of mass concentration 75% is added in 25 g/mL, and 10~15 min of ultrasonic extraction is filtered,
Filter residue repeats extraction 1~2 time, merges extracting solution, obtains cloves extracting solution, be concentrated under reduced pressure into no alcohol taste, upper D101 macroporous absorptions
Resin chromatography column removes macromolecular water soluble protein and polysaccharide with water elution, then successively with mass concentration 10%, 30%,
50%, 70%, 100% ethanol solution carries out gradient elution, collects the ethanol eluate at 30%~50% position, true at 60 DEG C
Sky is concentrated under reduced pressure, and obtains cloves chromocor extract；
（3）The preparation of mulberry leaf polyphenol extract：Mulberry leaf are collected, are dried under the conditions of 40~60 DEG C, are crushed, 200 mesh sieve is crossed；Then it presses
Solid-liquid ratio 1:20~1:The ethanol solution of mass concentration 70%, 30~45 min of ultrasonic extraction, filtering, filter residue is added in 30 g/mL
Extraction 1~2 time is repeated, merges extracting solution, obtains Mori folium extract, after being concentrated under reduced pressure into no alcohol taste, upper AB-8 macroporous absorptions tree
Fat chromatographic column removes macromolecular water soluble protein and polysaccharide with water elution, then successively with mass concentration 10%, 30%,
50%, 70%, 100% ethanol solution carries out gradient elution, collects the ethanol eluate at 50%~70% position, true at 60 DEG C
Sky is concentrated under reduced pressure, and obtains mulberry leaf polyphenol extract；
（4）The preparation of cassia seed anthraquinones extract：Cassia seed is dried through 40~60 DEG C, is crushed, and 200 mesh sieve is crossed；Then feed liquid is pressed
Than 1:10~1:The ethanol solution of mass concentration 90%, 30~45 min of ultrasonic extraction, filtering, filter residue repetition is added in 20 g/mL
Extraction 1~2 time merges extracting solution, obtains cassia seed extracting solution, after being concentrated under reduced pressure into no alcohol taste, upper AB-8 macroporous absorptions tree
Fat chromatographic column removes macromolecular water soluble protein and polysaccharide with water elution, then successively with mass concentration 20%, 40%,
60%, 80%, 100% ethanol solution carries out gradient elution, collects the ethanol eluate at 80% position, vacuum decompression at 60 DEG C
Concentration, obtains cassia seed anthraquinones extract；
（5）Compounding：Gained EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL, cloves chromocor extract, mulberry leaf polyphenol extract, cassia seed anthraquinone are carried
Object is taken to be mixed evenly in proportion with sucrose, Aspartame, acesulfame potassium, vitamin C, citric acid, purified water；
（6）Filling sterilizing：By prepared beverage filling in pop can bottle, sterilize 15-20 min at 115-120 DEG C, so
Postcooling obtains the plant weight losing function beverage containing Four Plants extract component to room temperature.
The remarkable advantage of the present invention is：
（1）The plant weight losing function beverage that important target spot of the present invention using fatty acid synthetase as weight-reducing makes is mainly with pomegranate
Skin and cloves, mulberry leaf, cassia seed these three medicinal and edible plants extract composition, multicomponent synergistic effect, effect is more
By force, and natural, safety, low toxicity.
（2）Preparation process of the present invention is simple, of low cost, and ethyl alcohol is only used during extraction and separation, does not make
It with other toxic solvents, not can cause environmental pollution, toxic solvents residue problem be also not present in extract, product quality can
It is effectively ensured.
（3）Drinks green natural of the present invention, sweet mouthfeel have faint scent, show that its total polyphenols contains through constituent analysis
Amount is 505 mg/100 g, general flavone content is 480 mg/100 g, total anthraquinones content is 265 mg/100 g, can play prevention
Fat health-care efficacy.
Specific implementation mode
In order to make content of the present invention easily facilitate understanding, With reference to embodiment to of the present invention
Technical solution is described further, but the present invention is not limited only to this.
Embodiment 1
（1）The preparation of EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL：Pericarpium granati, chopping are collected, the low temperature drying at 40 DEG C crushes, and crosses 200 mesh
Sieve；Then solid-liquid ratio 1 is pressed:The ethanol solution of mass concentration 60%, 5 min of ultrasonic extraction, filtering, filter residue weight is added in 15 g/mL
It extracts 1 time again, merges extracting solution, obtain pericarpium granati extracting solution, be concentrated under reduced pressure into no alcohol taste, upper D101 macroporous absorbent resins layer
Analyse column, macromolecular water soluble protein and polysaccharide removed with water elution, then successively with mass concentration 20%, 40%, 60%,
80%, 100% ethanol solution carries out gradient elution, collects the ethanol eluate at 60% position, vacuum-concentrcted at 60 DEG C,
Obtain EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL；
（2）The preparation of cloves chromocor extract：Cloves bud is collected, is dried under the conditions of 40 DEG C, is crushed, 120 mesh sieve is crossed；Then it presses
Solid-liquid ratio 1:The ethanol solution of mass concentration 75%, 10 min of ultrasonic extraction, filtering, filter residue repetition extraction 1 is added in 15 g/mL
It is secondary, merge extracting solution, obtain cloves extracting solution, is concentrated under reduced pressure into no alcohol taste, upper D101 macroporous adsorption resin chromatographies column, with washing
Macromolecular water soluble protein and polysaccharide are removed in removing, then successively with mass concentration 10%, 30%, 50%, 70%, 100%
Ethanol solution carries out gradient elution, collects the ethanol eluate at 30% position, and vacuum-concentrcted at 60 DEG C obtains cloves flavones
Extract；
（3）The preparation of mulberry leaf polyphenol extract：Mulberry leaf are collected, are dried under the conditions of 40 DEG C, are crushed, 200 mesh sieve is crossed；Then feed liquid is pressed
Than 1:The ethanol solution of mass concentration 70%, 30 min of ultrasonic extraction, filtering, filter residue repetition extraction 1 time, conjunction is added in 20 g/mL
And extracting solution, Mori folium extract is obtained, after being concentrated under reduced pressure into no alcohol taste, upper AB-8 macroporous adsorption resin chromatographies column, with water elution
Macromolecular water soluble protein and polysaccharide are removed, then successively with the second of mass concentration 10%, 30%, 50%, 70%, 100%
Alcoholic solution carries out gradient elution, collects the ethanol eluate at 50% position, and vacuum-concentrcted at 60 DEG C obtains mulberry leaf polyphenol and carries
Take object；
（4）The preparation of cassia seed anthraquinones extract：Cassia seed is dried through 40 DEG C, is crushed, and 200 mesh sieve is crossed；Then solid-liquid ratio 1 is pressed:
The ethanol solution of mass concentration 90% is added in 10 g/mL, and 30 min of ultrasonic extraction, filtering, filter residue repetition extraction 1 time, merging carries
Liquid is taken, cassia seed extracting solution is obtained, after being concentrated under reduced pressure into no alcohol taste, upper AB-8 macroporous adsorption resin chromatographies column, with water elution
Macromolecular water soluble protein and polysaccharide are removed, then successively with the second of mass concentration 20%, 40%, 60%, 80%, 100%
Alcoholic solution carries out gradient elution, collects the ethanol eluate at 80% position, and vacuum-concentrcted at 60 DEG C obtains cassia seed anthraquinone
Extract；
（5）Compounding：By EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 0.11%, cloves chromocor extract 0.30%, mulberry leaf polyphenol extract 0.18%,
Cassia seed anthraquinones extract 0.15%, sucrose 0.02%, Aspartame 0.01%, acesulfame potassium 0.01%, vitamin C 0.05%, lemon
Sour 0.015%, the ratio of purified water 99.155% is mixed evenly；
（6）Filling sterilizing：By prepared beverage filling in pop can bottle, sterilize 20 min at 115 DEG C, is subsequently cooled to
Room temperature obtains the plant weight losing function beverage containing Four Plants extract component.
Drinks obtained by the present embodiment are oral with 0.2 g/kg BW, 0.4 g/kg BW, the dosage of 0.8/kg BW
Gavage is given obese model mouse and is fed 30 days, the results show that the weight of each dosage group and fat/body ratio are significantly lower than model comparison
Group（P<0.05）, and the food ration of each dosage group is not significantly lower than model control group（P>0.05）, show to plant obtained by the present invention
Object weight losing function beverage has auxiliary weight losing function.
Embodiment 2
（1）The preparation of EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL：Pericarpium granati, chopping are collected, the low temperature drying at 50 DEG C crushes, and crosses 200 mesh
Sieve；Then solid-liquid ratio 1 is pressed:The ethanol solution of mass concentration 60%, 8 min of ultrasonic extraction, filtering, filter residue weight is added in 20 g/mL
It extracts 1 time again, merges extracting solution, obtain pericarpium granati extracting solution, be concentrated under reduced pressure into no alcohol taste, upper D101 macroporous absorbent resins layer
Analyse column, macromolecular water soluble protein and polysaccharide removed with water elution, then successively with mass concentration 20%, 40%, 60%,
80%, 100% ethanol solution carries out gradient elution, collects the ethanol eluate at 70% position, vacuum-concentrcted at 60 DEG C,
Obtain EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL；
（2）The preparation of cloves chromocor extract：Cloves bud is collected, is dried under the conditions of 50 DEG C, is crushed, 120 mesh sieve is crossed；Then it presses
Solid-liquid ratio 1:The ethanol solution of mass concentration 75%, 12 min of ultrasonic extraction, filtering, filter residue repetition extraction 1 is added in 20 g/mL
It is secondary, merge extracting solution, obtain cloves extracting solution, is concentrated under reduced pressure into no alcohol taste, upper D101 macroporous adsorption resin chromatographies column, with washing
Macromolecular water soluble protein and polysaccharide are removed in removing, then successively with mass concentration 10%, 30%, 50%, 70%, 100%
Ethanol solution carries out gradient elution, collects the ethanol eluate at 40% position, and vacuum-concentrcted at 60 DEG C obtains cloves flavones
Extract；
（3）The preparation of mulberry leaf polyphenol extract：Mulberry leaf are collected, are dried under the conditions of 50 DEG C, are crushed, 200 mesh sieve is crossed；Then feed liquid is pressed
Than 1:The ethanol solution of mass concentration 70%, 35 min of ultrasonic extraction, filtering, filter residue repetition extraction 1 time, conjunction is added in 25 g/mL
And extracting solution, Mori folium extract is obtained, after being concentrated under reduced pressure into no alcohol taste, upper AB-8 macroporous adsorption resin chromatographies column, with water elution
Macromolecular water soluble protein and polysaccharide are removed, then successively with the second of mass concentration 10%, 30%, 50%, 70%, 100%
Alcoholic solution carries out gradient elution, collects the ethanol eluate at 60% position, and vacuum-concentrcted at 60 DEG C obtains mulberry leaf polyphenol and carries
Take object；
（4）The preparation of cassia seed anthraquinones extract：Cassia seed is dried through 50 DEG C, is crushed, and 200 mesh sieve is crossed；Then solid-liquid ratio 1 is pressed:
The ethanol solution of mass concentration 90% is added in 15 g/mL, and 35 min of ultrasonic extraction, filtering, filter residue repetition extraction 1 time, merging carries
Liquid is taken, cassia seed extracting solution is obtained, after being concentrated under reduced pressure into no alcohol taste, upper AB-8 macroporous adsorption resin chromatographies column, with water elution
Macromolecular water soluble protein and polysaccharide are removed, then successively with the second of mass concentration 20%, 40%, 60%, 80%, 100%
Alcoholic solution carries out gradient elution, collects the ethanol eluate at 80% position, and vacuum-concentrcted at 60 DEG C obtains cassia seed anthraquinone
Extract；
（5）Compounding：By EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 0.20%, cloves chromocor extract 0.48%, mulberry leaf polyphenol extract 0.52%,
Cassia seed anthraquinones extract 0.28%, sucrose 0.04%, Aspartame 0.02%, acesulfame potassium 0.02%, vitamin C 0.1%, citric acid
0.02%, the ratio of purified water 98.32% is mixed evenly；
（6）Filling sterilizing：By prepared beverage filling in pop can bottle, sterilize 18min at 120 DEG C, is subsequently cooled to
Room temperature obtains the plant weight losing function beverage containing Four Plants extract component.
Embodiment 3
（1）The preparation of EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL：Pericarpium granati, chopping are collected, the low temperature drying at 60 DEG C crushes, and crosses 200 mesh
Sieve；Then solid-liquid ratio 1 is pressed:The ethanol solution of mass concentration 60%, 10 min of ultrasonic extraction, filtering, filter residue weight is added in 25 g/mL
It extracts 2 times again, merges extracting solution, obtain pericarpium granati extracting solution, be concentrated under reduced pressure into no alcohol taste, upper D101 macroporous absorbent resins layer
Analyse column, macromolecular water soluble protein and polysaccharide removed with water elution, then successively with mass concentration 20%, 40%, 60%,
80%, 100% ethanol solution carries out gradient elution, collects the ethanol eluate at 80% position, vacuum-concentrcted at 60 DEG C,
Obtain EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL；
（2）The preparation of cloves chromocor extract：Cloves bud is collected, is dried under the conditions of 60 DEG C, is crushed, 120 mesh sieve is crossed；Then it presses
Solid-liquid ratio 1:The ethanol solution of mass concentration 75%, 15 min of ultrasonic extraction, filtering, filter residue repetition extraction 2 is added in 25 g/mL
It is secondary, merge extracting solution, obtain cloves extracting solution, is concentrated under reduced pressure into no alcohol taste, upper D101 macroporous adsorption resin chromatographies column, with washing
Macromolecular water soluble protein and polysaccharide are removed in removing, then successively with mass concentration 10%, 30%, 50%, 70%, 100%
Ethanol solution carries out gradient elution, collects the ethanol eluate at 50% position, and vacuum-concentrcted at 60 DEG C obtains cloves flavones
Extract；
（3）The preparation of mulberry leaf polyphenol extract：Mulberry leaf are collected, are dried under the conditions of 60 DEG C, are crushed, 200 mesh sieve is crossed；Then feed liquid is pressed
Than 1:The ethanol solution of mass concentration 70%, 45 min of ultrasonic extraction, filtering, filter residue repetition extraction 2 times, conjunction is added in 30 g/mL
And extracting solution, Mori folium extract is obtained, after being concentrated under reduced pressure into no alcohol taste, upper AB-8 macroporous adsorption resin chromatographies column, with water elution
Macromolecular water soluble protein and polysaccharide are removed, then successively with the second of mass concentration 10%, 30%, 50%, 70%, 100%
Alcoholic solution carries out gradient elution, collects the ethanol eluate at 70% position, and vacuum-concentrcted at 60 DEG C obtains mulberry leaf polyphenol and carries
Take object；
（4）The preparation of cassia seed anthraquinones extract：Cassia seed is dried through 60 DEG C, is crushed, and 200 mesh sieve is crossed；Then solid-liquid ratio 1 is pressed:
The ethanol solution of mass concentration 90% is added in 20 g/mL, and 45 min of ultrasonic extraction, filtering, filter residue repetition extraction 2 times, merging carries
Liquid is taken, cassia seed extracting solution is obtained, after being concentrated under reduced pressure into no alcohol taste, upper AB-8 macroporous adsorption resin chromatographies column, with water elution
Macromolecular water soluble protein and polysaccharide are removed, then successively with the second of mass concentration 20%, 40%, 60%, 80%, 100%
Alcoholic solution carries out gradient elution, collects the ethanol eluate at 80% position, and vacuum-concentrcted at 60 DEG C obtains cassia seed anthraquinone
Extract；
（5）Compounding：By EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 0.52%, cloves chromocor extract 0.68%, mulberry leaf polyphenol extract 0.62%,
Cassia seed anthraquinones extract 0.38%, sucrose 0.08%, Aspartame 0.05%, acesulfame potassium 0.05%, vitamin C 0.20%, lemon
Sour 0.03%, the ratio of purified water 97.39% is mixed evenly；
（6）Filling sterilizing：By prepared beverage filling in pop can bottle, sterilize 15min at 120 DEG C, is subsequently cooled to
Room temperature obtains the plant weight losing function beverage containing Four Plants extract component.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification should all belong to the covering scope of the present invention.
Claims (2)
1. a kind of plant weight losing function beverage, it is characterised in that：The sum of weight percent is 100% meter, the plant slimming exercise
The weight percent of contained each ingredient is in energy beverage：EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 0.11~0.52%, cloves chromocor extract
0.30~0.68%, mulberry leaf polyphenol extract 0.18~0.62%, cassia seed anthraquinones extract 0.15~0.38%, sucrose 0.02~
0.08%, Aspartame 0.01~0.05%, acesulfame potassium 0.01~0.05%, vitamin C 0.05~0.20%, citric acid 0.015~
0.03%, remaining is purified water.
2. a kind of preparation method of plant weight losing function beverage as described in claim 1, it is characterised in that：Include the following steps：
（1）The preparation of EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL：Pericarpium granati, chopping are collected, the low temperature drying at 40~60 DEG C crushes, mistake
200 mesh sieve；Then solid-liquid ratio 1 is pressed:15~1:The ethanol solution of mass concentration 60%, ultrasonic extraction 5~10 is added in 25 g/mL
Min, filtering, filter residue repeat extraction 1~2 time, merge extracting solution, obtain pericarpium granati extracting solution, be concentrated under reduced pressure into no alcohol taste, on
D101 macroporous adsorption resin chromatography columns remove macromolecular water soluble protein and polysaccharide, then with mass concentration with water elution
The ethanol solution of 20%-100% carries out gradient elution, collects the ethanol eluate at 60%~80% position, and vacuum subtracts at 60 DEG C
Pressure concentration, obtains EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL；
（2）The preparation of cloves chromocor extract：Cloves bud is collected, is dried under the conditions of 40~60 DEG C, is crushed, 120 mesh sieve is crossed；So
Solid-liquid ratio 1 is pressed afterwards:15~1:The ethanol solution of mass concentration 75% is added in 25 g/mL, and 10~15 min of ultrasonic extraction is filtered,
Filter residue repeats extraction 1~2 time, merges extracting solution, obtains cloves extracting solution, be concentrated under reduced pressure into no alcohol taste, upper D101 macroporous absorptions
Resin chromatography column removes macromolecular water soluble protein and polysaccharide, then with the second of mass concentration 10%-100% with water elution
Alcoholic solution carries out gradient elution, collects the ethanol eluate at 30%~50% position, and vacuum-concentrcted at 60 DEG C obtains cloves
Chromocor extract；
（3）The preparation of mulberry leaf polyphenol extract：Mulberry leaf are collected, are dried under the conditions of 40~60 DEG C, are crushed, 200 mesh sieve is crossed；Then it presses
Solid-liquid ratio 1:20~1:The ethanol solution of mass concentration 70%, 30~45 min of ultrasonic extraction, filtering, filter residue is added in 30 g/mL
Extraction 1~2 time is repeated, merges extracting solution, obtains Mori folium extract, after being concentrated under reduced pressure into no alcohol taste, upper AB-8 macroporous absorptions tree
Fat chromatographic column removes macromolecular water soluble protein and polysaccharide, then with the ethyl alcohol of mass concentration 10%-100% with water elution
Solution carries out gradient elution, collects the ethanol eluate at 50%~70% position, and it is more to obtain mulberry leaf for vacuum-concentrcted at 60 DEG C
Phenol extraction object；
（4）The preparation of cassia seed anthraquinones extract：Cassia seed is dried through 40~60 DEG C, is crushed, and 200 mesh sieve is crossed；Then feed liquid is pressed
Than 1:10~1:The ethanol solution of mass concentration 90%, 30~45 min of ultrasonic extraction, filtering, filter residue repetition is added in 20 g/mL
Extraction 1~2 time merges extracting solution, obtains cassia seed extracting solution, after being concentrated under reduced pressure into no alcohol taste, upper AB-8 macroporous absorbent resins
Chromatographic column removes macromolecular water soluble protein and polysaccharide with water elution, then molten with the ethyl alcohol of mass concentration 20%-100%
Liquid carries out gradient elution, collects the ethanol eluate at 80% position, vacuum-concentrcted at 60 DEG C, obtains the extraction of cassia seed anthraquinone
Object；
（5）Compounding：Gained EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL, cloves chromocor extract, mulberry leaf polyphenol extract, cassia seed anthraquinone are carried
Object is taken to be mixed evenly in proportion with sucrose, Aspartame, acesulfame potassium, vitamin C, citric acid, purified water；
（6）Filling sterilizing：By prepared beverage filling in pop can bottle, sterilize 15-20 min at 115-120 DEG C, so
Postcooling obtains the plant weight losing function beverage to room temperature.
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