CN108478703A

CN108478703A - A kind of Chinese medicine composition and quality determining method for treating arrhythmia cordis

Info

Publication number: CN108478703A
Application number: CN201810675116.3A
Authority: CN
Inventors: 张艳; 张彦; 王超凡; 成泽东
Original assignee: Liaoning University of Traditional Chinese Medicine
Current assignee: Liaoning University of Traditional Chinese Medicine
Priority date: 2018-06-27
Filing date: 2018-06-27
Publication date: 2018-09-04

Abstract

A kind of Chinese medicine composition and quality determining method for treating arrhythmia cordis, including Chinese medicine major ingredient and pharmaceutic adjuvant, the Chinese medicine major ingredient are made of following ingredients weight parts proportioning Chinese medicine：Honey-fried licorice root, Radix Rehmanniae, ginseng, Radix Astragali, Radix Ophiopogonis, Radix Salviae Miltiorrhizae, safflower, spina date seed.Preparation method includes the following steps：Honey-fried licorice root, Radix Rehmanniae, ginseng, Radix Astragali are taken in prescription ratio, twice, filtering, filtrate merges heating and refluxing extraction；Radix Ophiopogonis, Radix Salviae Miltiorrhizae, safflower, spina date seed are taken in prescription ratio, the gained dregs of a decoction are added, obtains liquid, distillate is collected, carries out coarse filtration and refined filtration with plate and frame filter press, concentrate spray drying process prepares extract powder；Dextrin mixing is added in cream powder, is pelletized with ethanol wet, wet granular boiled bed drying, whole grain, both obtains finished dosage form.The invention can adjust the compliance of patient's cardiac muscle, reduce intra-atrial reentry, stablize myocardial cell membrane, improve the multipole conducting system function of cardiac muscle, improve the quality of life of patient, will be widely used in technical field of Chinese medicine.

Description

A kind of Chinese medicine composition and quality determining method for treating arrhythmia cordis

Technical field

The present invention relates in technical field of Chinese medicine a kind of Chinese medicine composition that treating arrhythmia cordis and quality testing side Method.

Background technology

Currently, arrhythmia cordis is since sinoatrial node excitement exception or excitement result from other than sinoatrial node, exciting conduction is slow Slowly, block or conducted through abnormal passage, i.e., the origin of cardiomotility and (or) conductive impairment cause heartbeat frequency and (or) allorhythmia.Arrhythmia cordis is one group of disease important in angiocardiopathy.It can individually fall ill, also can be with other painstaking effort Pipe disease occurs together.Whether its prognosis and the cause of disease of arrhythmia cordis inducement, evolving trend, cause serious hemodynamic obstacle related, can It breaks out and causes to die suddenly, it is also sustainable to involve heart and cause its failure.Premature beat is the most common type arrhythmia cordis.Premature beat is again Referred to as Premature contracfion.It refer to heartbeat caused by the too early impulsion that ectopic pacemaker is sent out.Sinus property or atopy can be happened at On the basis of (such as auricular fibrillation) rhythm of the heart.It can be accidental or takes place frequently, it can be irregularly or regular at each or per several normal Occur after beating, forms bigeminy or connection rule property Premature contracfion.It can be divided into sinus property, Fang Xing, atrioventricular junction by origin position With four kinds of room property.Wherein most common with ventricular premature beat, followed by room, knot property are more rare.Arrhythmia cordis is in angiocardiopathy One group of important disease.It can individually fall ill, and can also occur together with other cardiovascular diseases.The cause of disease of its prognosis and arrhythmia cordis lures Whether cause, evolving trend cause serious hemodynamic obstacle related, can break out and cause to die suddenly, also sustainable to involve heart And cause its failure.

The classification of antiarrhythmic drug has been continued to use nearly 30 years.Drug is divided into according to pharmaceutically-active electro physiology feature Four classes.I class blocks the sodium channel of cardiac muscle and conducting system of heart, has membrane stabilizing action, reduces by 0 phase depolarization of action potential and rises Rate and amplitude slow down conduction of velocity, over reach potential duration (APD) and event related potential (ERP).To resting membrane electric potential Without influence.According to drug to the difference of sodium channel retardation, it is divided into as three subclass, i.e. I a, Ib, I c.

(1) I a class appropriateness block sodium channels bring back to life time constant 1-10s, and the most notable to extend ERP, drug includes Kui Buddhist nun's fourth, procainamide, disopyramide etc..

(2) the I slight block sodium channels of b classes bring back to life time constant<1s reduces self-disciplining, and drug includes lidocaine, benzene Appropriate English sodium, mexiletine etc..

(3) the I apparent block sodium channels of c classes bring back to life time constant>It is most strong to slow down conductive effect by 10s.Drug includes Propafenone, Encainide, Flecainide etc..

II class Bextra inhibits pacemaker current, sodium current and L-type calcium current caused by sympathetic activation to increase Add, show as slowing down four phase diastole depolarization rates and reducing self-disciplining, reduces by 0 phase climbing speed of action potential and slow down conduction Property.Drug includes Propranolol, atenolol, metoprolol etc..

III class over reach potential duration medicine, inhibits a variety of potassium currents, drug include amiodarone, Sotalol, bretylium tosylate, According to cloth for sharp and how non-for profit etc..

IV class calcium channel blocker, including Verapamil and diltiazem etc..

It is noted that grasping the indication of antiarrhythmic drug in clinic, and not all arrhythmia cordis is both needed to apply the anti-heart Arrhythmic agents are restrained, only directly result in apparent symptom or haemodynamics obstacle or with the malignant cardiac rhythm for causing fatal risk It just needs to be treated for arrhythmia cordis when not normal.Antiarrhymic has inevitably bad anti-in clinical application It answers.Such as：Nausea,vomiting,diarrhea, abdominal pain, cacesthesia, clouding of consciousness, delirium, stupor, aggravation asthma and chronic obstructive pulmonary The adverse reactions such as disease, intermittent claudication, Raynaud's phenomenon, mental depression, bradycardia.Therefore, development is a kind for the treatment of the heart It restrains not normal Chinese medicine composition and quality determining method is always new issue anxious to be resolved.

Invention content

The purpose of the present invention is to provide a kind of Chinese medicine composition and quality determining method for treating arrhythmia cordis, the inventions Palatelet-selectin in rat plasma can be reduced, thromboxane B2 (TXB2), matrix metalloproteinase 9 in atrial fibrillation rat blood serum can be reduced (MMP-9) content lowers plasminogen activator inhibitor (PAI-I) level in rat blood serum, to mitigate the breaking-out of atrial fibrillation, The Ca of cardiac muscular tissue can be lowered²⁺ATP enzyme genetic transcription reaches calcium overload when improving atrial fibrillation, delays the hair of atrial electrical remodeling It is raw, the effect of " AF causes AF " is prevented, Cx40 gene expression doses can be substantially reduced, the multiple generation turned back in atrium can be reduced, The expression for adjusting kinases 2 (ERK2) mRNA outside atrial fibrillation rat heart muscle histocyte can be raised, atrial fibrillation rat can be lowered Cardiac muscular tissue's L-type calcium channel mRNA expression is horizontal, and is effectively improved the variation of atrial fibrillation myocardial ultrastructure.

The object of the present invention is achieved like this：A kind of Chinese medicine composition for treating arrhythmia cordis, including Chinese medicine major ingredient and Pharmaceutic adjuvant, the Chinese medicine major ingredient are made of following ingredients weight parts proportioning Chinese medicine：Honey-fried licorice root 25-40g, Radix Rehmanniae 25- 30g, ginseng 20-25g, Radix Astragali 25-40g, Radix Ophiopogonis 25-40g, Radix Salviae Miltiorrhizae 35-40g, safflower 20-25g, spina date seed 20-40g；

The Chinese medicine major ingredient is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 30g, Radix Rehmanniae 25g, people Join 20g, Radix Astragali 30g, Radix Ophiopogonis 25g, Radix Salviae Miltiorrhizae 35g, safflower 20g, spina date seed 20g；

The Chinese medicine major ingredient is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 30g, Radix Rehmanniae 25g, people Join 25g, Radix Astragali 25g, Radix Ophiopogonis 25g, Radix Salviae Miltiorrhizae 35g, safflower 20g, spina date seed 25g；

The Chinese medicine major ingredient is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 25g, Radix Rehmanniae 30g, people Join 20g, Radix Astragali 35g, Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 35g, safflower 20g, spina date seed 20g；

The Chinese medicine major ingredient is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 25g, Radix Rehmanniae 25g, people Join 20g, Radix Astragali 30g, Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 40g, safflower 20g, spina date seed 25g；

The Chinese medicine major ingredient is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 30g, Radix Rehmanniae 25g, people Join 20g, Radix Astragali 30g, Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 35g, safflower 25g, spina date seed 25g；

The Chinese medicine major ingredient is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 40g, Radix Rehmanniae 25g, people Join 25g, Radix Astragali 30g, Radix Ophiopogonis 25g, Radix Salviae Miltiorrhizae 35g, safflower 20g, spina date seed 25g；

The Chinese medicine major ingredient is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 25g, Radix Rehmanniae 25g, people Join 20g, Radix Astragali 30g, Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 35g, safflower 25g, spina date seed 40g；

The Chinese medicine major ingredient is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 35g, Radix Rehmanniae 30g, people Join 25g, Radix Astragali 25g, Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 35g, safflower 20g, spina date seed 30g；

The Chinese medicine major ingredient is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 35g, Radix Rehmanniae 30g, people Join 20g, Radix Astragali 25g, Radix Ophiopogonis 25g, Radix Salviae Miltiorrhizae 40g, safflower 20g, spina date seed 40g；

The pharmaceutic adjuvant is one or more of in dextrin, starch, lactose；

A kind of preparation method of Chinese medicine composition for treating arrhythmia cordis includes the following steps：

(1) honey-fried licorice root, Radix Rehmanniae, ginseng, Radix Astragali are taken in prescription ratio, 70% ethyl alcohol measured with 10 times is heated to reflux and carries It takes twice, 2 hours every time, filters, filtrate merges, and recycling ethyl alcohol obtains liquid I；

(2) Radix Ophiopogonis, Radix Salviae Miltiorrhizae, safflower, spina date seed are taken in prescription ratio, impregnated 30 minutes, the gained dregs of a decoction in step (1) are added Entering, common decocting 2 times, for the first time 12 times of water, decocts 2 hours, filtration obtains liquid II, and second of 8 times of water decocts 1.5 hours, Filtration, obtains liquid III, collects distillate twice, obtain liquid IV；

(3) liquid I, II, III, IV is merged, mixing carries out coarse filtration and refined filtration, refined filtration liquid triple effect with plate and frame filter press It is 1.15 that energy-saving concentrator, which is concentrated into relative density, temperature 60 C；Concentrate spray drying process, 6000 milliliters of feed speed/ Minute, 6000 revs/min of centrifugal speed, 120 DEG C of inlet air temperature prepares extract powder；20% dextrin mixing is added in cream powder, uses 80% ethanol wet is pelletized, wet granular boiled bed drying, 75 DEG C of wind-warm syndrome；Beta-cyclo dextrin included compound powder, whole grain is added；It examines and divides Loaded in aluminium plastic membrane bag, 3 grams are often wrapped, both obtains finished dosage form；

Preparation in the step (3) is pill, capsule, decoction, powder, electuary, particle or oral solution；

A kind of quality determining method of Chinese medicine composition for treating arrhythmia cordis is as follows：

One, instrument and reagent

LC-10P liquid phase pump LC-10UV UV detector, big Liaanjiang county Shen separation science Technology Co., Ltd. production；Chromatographic column 1(Hypersil)：Dalian Yi Lite Co., Ltds provide；5 μ, 4.6mm × 200mm；Chromatographic column 2 (Spherigel)：Big Liaanjiang county Shen separation science Co., Ltd provides；5 μ, 4.6mm × 200mm；Agents useful for same acetonitrile is chromatographically pure, and Shandong king's Yu industry is limited Company Yucheng chemical plant；Water is double distilled water, and other reagents are that analysis is pure；Ginsenoside Rg1's reference substance, Chinese drug biology system Product examine is fixed to be provided, and assay is used, lot number 0703-9812；

Two, method and result

1, thin layer differentiates

(1) thin layer of Radix Salviae Miltiorrhizae differentiates

This product content 2g is taken, water 50ml, ultrasonic extraction 30 minutes is added to let cool, centrifuges, supernatant is taken, with dilute hydrochloric acid tune Section pH value is 1-2, is extracted 2 times, each 20ml with ethyl acetate, merges ethyl acetate liquid, is evaporated, it is molten that residue adds methanol 1ml to make Solution, as test solution；Protocatechualdehyde reference substance separately is taken, adds methanol that solution of every 1ml containing 1mg is made, product are molten as a contrast Liquid；It is tested according to thin-layered chromatography, with reference to Chinese Pharmacopoeia one annex VIB of version in 2000, draws 5 μ l of reference substance solution, test sample Solution 10-20 μ l, put on the silica gel g thin-layer plate in same using 0.4% sodium carboxymethylcellulose as binder, respectively with chloroform- Acetone-formic acid is solvent, chloroform：Acetone：The ratio of formic acid is 8：1：1, it is unfolded, takes out, dry, sprays with 5% ferric trichloride Ethanol solution, it is clear that hot wind is blown to spot development, sets and is inspected under daylight；In test sample chromatography, corresponding with reference substance chromatography On position, the spot of same color is shown；

(2) thin layer of Radix Astragali differentiates

This product content 3g is taken, methanol 30ml is added, is ultrasonically treated 30 minutes, lets cool, is filtered, filtrate is evaporated, and residue adds water 20ml makes dissolving, is transferred in separatory funnel, adds water saturated n-butanol shaking extraction 2 times, is followed successively by 30ml, 20ml, merges N-butanol extracting liquid is washed 2 times, each 20ml with 1% sodium hydroxide solution, discards lye, then the washing being saturated with n-butanol It washs 2 times, each 15ml, discards aqueous, n-butanol liquid is evaporated, and residue adds methanol 1-2ml to make dissolving, as test solution；Separately Astragaloside IV reference substance is taken, adds methanol that solution of every 1ml containing 0.5mg is made, as a contrast product solution；It is tried according to thin-layered chromatography It tests, with reference to one VI B of annex of China's coastal port, draws each 5 μ l of above two solution, put respectively thin in same silica G On laminate, using 10 DEG C of overnight lower layer's solution arranged below of chlorofonn-ethylacetate-methanol-water as solvent, chloroform：Acetic acid second Ester：Methanol：The ratio of water is 15: 40: 22: 10, is unfolded, and takes out, dries, and spray is heated with 10% ethanol solution of sulfuric acid, at 105 DEG C It is clear to spot development；In test sample chromatography, on position corresponding with reference substance chromatography, the spot of same color is shown；

(3) thin layer of spina date seed differentiates

This product content 4g is taken, water 50ml, ultrasonic extraction 30 minutes is added to let cool, centrifuges, takes supernatant, use water saturated N-butanol extracts three times, and each 25ml merges n-butanol liquid, is extracted 2 times, each 50ml with ammonia solution, discards ammonia solution, positive fourth Alcohol liquid is extracted 2 times, each 30ml with distilled water, discards aqueous solution, and n-butanol liquid is evaporated, and residue adds methanol 1ml to make dissolving, as Test solution；Saponin A reference substance separately is taken, adds methanol that solution of every 1ml containing 1mg is made, as a contrast product solution.According to Thin-layered chromatography is tested, and with reference to Chinese Pharmacopoeia version one in 2000, annex VI B draw each 5-10 μ l of above two solution, point Other point is on same silica gel g thin-layer plate, using 10 DEG C of lower layer's solution arranged below of chloroform-methanol-water as solvent, chloroform:First Alcohol:The ratio of water is 65：35：10, it is unfolded, takes out, dry, sprays with 1% vanillic aldehyde concentrated sulfuric acid solution, hot wind is blown to spot development Clearly, it sets and is inspected under daylight；In test sample chromatography, on the corresponding position of product chromatography, the spot of same color 1 is shown；

2, assay

(1) chromatographic condition：

Chromatographic column 1 (Hypersil)：5 μ, 4.6mm × 200mm；Chromatographic column 2 (Spherigel)：5 μ, 4.6mm × 200mm； It is filler with octadecylsilane chemically bonded silica；- 0.05% phosphoric acid of acetonitrile is mobile phase；Acetonitrile：The ratio of 0.05% phosphoric acid It is 20：80, Detection wavelength 203nm；

(2) preparation of reference substance solution:

Precision weighs ginsenoside Rg1 reference substance 10.78mg, is placed in 10ml volumetric flasks, methanol is added to make it dissolve in right amount And it is diluted to scale, product solution as a contrast after shaking up；Precision draws above-mentioned reference substance solution 2.5ml, is placed in 5ml volumetric flasks In, add methanol dilution to scale, product dilute solution as a contrast after shaking up, a concentration of 0.539mg/ml of dilute solution；

(3) preparation of test solution：

The a small amount of content of this product is taken, it is finely ground, take 2g, it is accurately weighed and water-saturated n-butanol 50ml is added, it is weighed heavy again Amount, ultrasonic extraction 30 minutes are stood overnight, and twice ultrasonic extracts 30 minutes, and weighed weight supplies less loss with water-saturated n-butanol Weight, filtration discards primary filtrate, collects subsequent filtrate；Precision draws subsequent filtrate 25ml, sets in separatory funnel, with 0.5% hydrogen Aqueous solution of sodium oxide washs 2 times, each 10ml, merges butanol solution, and the water 15ml being saturated with n-butanol is washed 1 time, is discarded Water lotion, butanol solution water bath method；Residue adds methanol to make to be dissolved in 25ml measuring bottles, adds methanol dilution to scale, shakes up, It is filtered to obtain the final product with miillpore filter, empty a diameter of 0.45 μm of the net of miillpore filter；

(4) selection of Detection wavelength：

Ginsenoside Rg1's reference substance solution 50 μ l, solution concentration 1.078mg/ml are taken, adds methanol about 5ml, uses Shimadzu UV-260 is scanned in 400-200nm wave-length coverages, and UV absorption figure is shown in Fig. 1, can be seen that ginseng from UV absorption Fig. 1 Saponin(e Rg1 has absorption peak in 203nm；Therefore select 203nm for ginsenoside Rg1's assay wavelength；

(5) ginsenoside Rg1's reference substance linear relationship is investigated：

Accurate 2 μ l of ginsenoside Rg1's reference substance dilute solution, 4 μ l, 6 μ l, 8 μ l and 10 μ l, the solution concentration drawn is respectively 1.078mg/ml is injected separately into liquid chromatograph, measures ginsenoside Rg1's chromatographic peak peak area, sample size and peak area Relationship is shown in Table 1；

1 ginsenoside Rg1's reference substance sample size of table and peak area relationship

Sample size μ g	2.156	4.312	6.468	8.624	10.078
						Peak area	10202387	22357595	32868559	45702492	56099805

Calibration curve equation is Y=9.163 × 10^-8X+1.074×10^-2R=0.9997；

Sample introduction is ranging from：2.156 μ of μ g~10.078 g；

(6) precision test：

20,090801 sample of lot number is taken, it is finely ground, 2g is taken, it is accurately weighed, by test liquid preparation side under " assay " item Method is tested, and precision draws 5 μ l of test liquid, is amounted to 5 parts, is injected separately into liquid chromatograph, and ginsenoside Rg1's peak area point is measured Not Wei 20254816,20722368,19968224,20929208,20274486, relative standard deviation 1.90%；

(7) blank test：

Recipe quantity medicinal material is taken, ginseng is removed, negative controls are made by preparation process, then press and tried under " assay " item It tests, prepares the negative controls solution for lacking ginseng crude drug；By negative controls solution, test solution and each 5 μ of reference substance solution L is injected separately into liquid chromatograph, and measurement result shows that negative fluid has no phase at ginsenoside Rg1's chromatographic peak retention time Impurity peaks are answered to interfere；

(8) stability test：

20,090801 sample of lot number is taken, it is finely ground, 2g is taken, it is accurately weighed, by test liquid preparation side under " assay " item Method is tested, and when test liquid is placed 0,4,8 and 12 hour, draws test liquid and each 5 μ of ginsenoside Rg1's reference substance solution L is injected separately into liquid chromatograph, and it is respectively 20254816,20557692,20527968,20977204 to measure peak area, phase It is 1.45% to standard deviation；From test result it is found that test liquid is placed 12 hours, Determination of Content of Ginsenoside Rg_1 is basicly stable.From The above results are it is found that this assay method, test solution precision are good；

(9) reappearance test：

20,090801 sample of lot number is taken, it is finely ground, 2g is taken, it is accurately weighed, by test liquid preparation side under " assay " item Method is tested, Determination of Content of Ginsenoside Rg_1 in determination sample, and result is：8.86,8.98,9.08,9.02,8.80mg/, averagely contain Amount is 8.94mg/, relative standard deviation 1.29%；From test result it is found that Determination of Content of Ginsenoside Rg_1 measures reproducibility examination It tests and meets regulation；

(10) sample recovery rate is tested：

20,090801 sample of lot number is taken, it is finely ground, 1g is taken, it is accurately weighed, by test liquid preparation side under " assay " item Method is tested, and amounts to 5 parts, and difference is accurate to be added ginsenoside Rg1 reference substance 17mg, by being tested under " assay " item, measures and adds Determination of Content of Ginsenoside Rg_1 in standard specimen product calculates, obtains the rate of recovery of this content assaying method ginsenoside Rg1；Its test result It is shown in Table 2；

2 Determination of Content of Ginsenoside Rg_1 of table measures recovery test result

From above-mentioned recovery test result it is found that this method rate of recovery is good；

(11) Determination of Content of Ginsenoside Rg_1 measures in sample:

Each 20 of the sample of different lot numbers is taken, it is finely ground, 2g is taken, it is accurately weighed, by method test under " assay " item, survey Determine Determination of Content of Ginsenoside Rg_1, calculates, the result is shown in tables 3；

Determination of Content of Ginsenoside Rg_1 measurement result in 3 sample of table amounts to 10 batches

Determination of Content of Ginsenoside Rg_1 in sample is can be seen that in 5.61mg/ -9.88mg/ grains from above-mentioned test result Between, therefore ginseng will be defined as in finished product must not be less than 5.0mg in terms of ginsenoside Rg1；

Three, conclusion

1, pharmacodynamic action

A kind of Chinese medicine composition that treating arrhythmia cordis is formed by zhigancao decoction addition or subtraction of changes；Zhigancao decoction derives from Zhang Zhong Scape《The Treatise on Fevrile Diseases》, " typhoid fever, intermittent pulse severe palpilation, zhigancao decoction master it " primary efficacy be benefiting qi and nourishing yin, activate yang multiple arteries and veins；It is a kind of The Chinese medicine composition for treating arrhythmia cordis is by eight taste such as Radix Rehmanniae, ginseng, Radix Astragali, Radix Ophiopogonis, Radix Glycyrrhizae, spina date seed, Radix Salviae Miltiorrhizae, safflower Medicine forms；Research through modern pharmacology, a kind of Chinese medicine composition that treating arrhythmia cordis have good antiarrhythmic work With；Honey-fried licorice root：Effect is invigorating middle warmer and replenishing QI, and relieving spasm to stop pain moistens the lung and relieve the cough, purging intense heat and detonicating, mediation property of medicine；It is sweet in flavor mild-natured, returns spleen, Stomach, the heart, lung channel；Gas and property are slow, and can rise can drop；Modern medicine study, honey-fried licorice root have apparent antiarrhythmic effect, sugared skin Matter hormone-like effect, anti-oxidant and anti-aging effects；Radix Rehmanniae：Effect is nourishing Yin and clearing heat, and cool blood is enriched blood.It is sweet in flavor, bitter, it is slightly cold, returns The heart, liver, stomach, the thick drop of matter are let out；Myocardial blood flow amount, reducing heart rate can be increased, and there is certain antihypertensive effect；It is yellow Stilbene：Function be it is raw with can invigorating qi for consolidating superficies, inducing diuresis for removing edema；Toast with can tonifying middle-Jiao and Qi, control all laboured breathing syndrome of blood deficiency；Nature and flavor, it is sweet, it is micro- Temperature enters lung, the spleen channel；It can significantly improve heart discharge, cardiac index, the often amount of fighting and index of often fighting；Ginseng：It can reinforce vital energy, Calm the nerves, internal lesion caused by overexertion it is deficient, deficiency of food, the card of all qi and blood sperm deficiencies such as burnout；Nature and flavor：It is sweet, slight bitter, temperature；Enter spleen, lung channel；Energy Enhance myocardial contractive power, increase cardiac output, there is cardiac effect；Increase coronary blood flow, reducing heart rate, resist myocardial ischemia work With anti anoxia and protection cardiac muscle reduce myocardial oxygen consumption, reduce affinity of the hemoglobin to oxygen, more to tissue release Oxygen；Radix Ophiopogonis：Effect is nourishing Yin and moistening lung, beneficial stomach production of sperm, relieving restlessness that clears away heart-fire；Nature and flavor are sweet, slight bitter, cold nature, return lung, stomach, the heart channel of Hang-Shaoyin；It improves Myocardial contractive power and cardiac pumping function improve tolerance of the mouse ischemic myocardium to bottom oxygen, improve cardiac muscle cell's nutrition, blood flow Amount, anti-oxidant, antifatigue effect promote liquid immune；Thus a kind of Chinese medicine composition for treating arrhythmia cordis is clinically Play the role for the treatment of arrhythmia cordis well；

2, detection method result

This test result establishes a kind of quality determining method of Chinese medicine composition that treating arrhythmia cordis, and ginseng is one kind The main ingredient of the Chinese medicine composition of arrhythmia cordis, ginsenoside Rg1 containing active ingredient are treated, and content is higher, as in said preparation Main component, content directly influences therapeutic effect, therefore uses high performance liquid chromatography, using it as the finger of assay Ingredient is marked, can reflect the quality of said preparation, a kind of Chinese medicine composition that treating arrhythmia cordis is made with the content of ginsenoside Rg1 It is reasonable for the index of quality control；Simultaneously using according to thin-layered chromatography, to spina date seed in preparation, Radix Salviae Miltiorrhizae, Radix Astragali is determined Property differentiate, as a result on position corresponding with reference substance chromatography, show same color spot, method favorable reproducibility；The quality is examined The foundation of survey method is for reducing preparation differences between batches, ensureing the quality of the pharmaceutical preparations, ensure that clinical efficacy is of great significance, and method Easy to operate, accurate, reproducible, specificity is strong, can be used for controlling the quality of Dingxin capsule.

The present invention is characterized by its Chinese medicine composition proportioning, preparation method and quality determining method.Its principle is：It visits Study carefully a kind of quality determining method of Chinese medicine composition that treating arrhythmia cordis, a kind of Chinese medicine composition that treating arrhythmia cordis is to face Empirical formula of the bed for treating arrhythmia cordis more than two decades.By in eight taste such as Radix Rehmanniae, ginseng, Radix Astragali, Radix Ophiopogonis, Radix Glycyrrhizae, spina date seed The effect of medicine compatibility forms, and there is invigorating heart gas to help heart-yang, grows the heart-yin nourishing heart blood, and heart arteries and veins is answered in promoting blood circulation.Preferably to control in it In quality and clinical efficacy, we are with a kind of active ingredient ginseng soap of main ingredient ginseng in Chinese medicine composition for treating arrhythmia cordis Glycosides Rg1 is index, using the content of Radix Ginseng by HPLC saponin(e Rg1, while to Radix Salviae Miltiorrhizae in preparation, Radix Astragali, acid Three taste medicine of jujube kernel carries out thin-layer qualitative discriminating, and foundation is provided for the complete quality standards in Chinese drugs of determination.

Chinese medicine has significant advantage to the correction of arrhythmia cordis, and treatment arrhythmia cordis is significant in efficacy, before having wide development Scape.Without serious adverse reaction." monarch " combination principle of Chinese medicine：《Sheng Nong's herbal classic》：" 120 kinds of medicine-feeding is monarch, It is main to support life；120 kinds of Chinese medicine is minister, and master nourishs one's nature；Putting in poison 120 kinds makes for assistant, cures mainly disease；Medication must close the monarch and his subjects' assistant Make." monarch drug in a prescription：It is the drug for main disease or main symptom its primary treatment effect.Ministerial drug：It is that auxiliary monarch drug in a prescription reinforces the main disease for the treatment of, master The drug of card.Adjutant：It is the therapeutic effect for reinforcing monarch, ministerial drug, supervises its toxicity and high strong property, or prevent seriously ill heresy Very, the drug of expulsion of yin-yang.Make medicine：Drug with tying-in and reconciliation effect in the side of being.Among one side, monarch drug in a prescription must be indispensable, And minister, help, make three medicines then can take the circumstances into consideration configure or delete.Monarch minister, helps, makes --- and it is the group that tcm prescription medication must comply with Knit principle, be it is dialectical, legislation on the basis of, according to the needs of the state of an illness, the principle that comedicine is combined.It is every according to performance Herbal medicine is different.

According to clinical practice and theoretical research, from quasi- fixed core square, medicine group becomes：Honey-fried licorice root, Radix Rehmanniae, ginseng, Radix Astragali, Radix Ophiopogonis, Radix Salviae Miltiorrhizae, safflower, spina date seed.In the application, it is different according to the different compatibilities of illness.Premature beat point functionality and device matter Property, concerted application of drugs is different, and functional premature beat spina date seed dosage is big, organic premature beat ginseng, Radix Astragali, Radix Salviae Miltiorrhizae dosage Greatly.By multiple zoopery and clinical test, nine kinds of proportioning dosage are proposed according to Chinese prescription principle, wherein zoopery Seven kinds of Chinese prescriptions, two kinds of Chinese prescriptions of clinical test are in varied situations applied to premature beat, the treatment of atrial fibrillation.Chinese medicine What is do not passed secret is in dosage ", it is seen that drug dose is an important factor for influencing compound effect, is clinical practice of Chinese medicine experience product Tired crystallization embodies tcm clinical practice medicine law, is tcm treatment according to syndrome differentiation, the side's of sending medication and the material base for characterizing curative effect. The it is proposed of this nine kinds of combinations is in the case where curing mainly card and the interpretation of the cause, onset and process of an illness is essentially identical, by the change of drug dose, but compatibility Relationship is constant, and treatment state of an illness weight is different, and curative effect also changes, and produces various clinical effect.

A kind of Chinese medicine composition that treating arrhythmia cordis is demonstrated by having done a large amount of clinical and animal experiment study Curative effect, by the economic benefit brought and social benefit.Multinomial data prove a kind of Chinese medicine composition for treating arrhythmia cordis The general status of atrial fibrillation rat can be improved；Palatelet-selectin content increases in blood plasma when atrial fibrillation, and platelet activation, the present invention can Reduce palatelet-selectin in rat plasma；The content of TXB2, MMP-9 in atrial fibrillation rat blood serum can be reduced；It lowers in rat blood serum PAI-I is horizontal, to mitigate the breaking-out of atrial fibrillation；A kind of Chinese medicine composition for treating arrhythmia cordis can lower the Ca of cardiac muscular tissue²⁺- ATP enzyme genetic transcription reaches calcium overload when improving atrial fibrillation, to delay the generation of atrial electrical remodeling, prevents the work of " AF causes AF " With；A kind of Chinese medicine composition that treating arrhythmia cordis can be substantially reduced Cx40 gene expression doses, can reduce the multiple folding in atrium The generation returned；The expression that kinases 2 (ERK2) mRNA is adjusted outside atrial fibrillation rat heart muscle histocyte can be raised；It can lower Atrial fibrillation rat heart muscle tissue L-type calcium channel mRNA expression is horizontal；And it is effectively improved the variation of atrial fibrillation myocardial ultrastructure. Clinical observation proves that a kind of Chinese medicine composition for treating arrhythmia cordis can improve and restore the overall effective of patient's ventricular late potential Rate up to 79.9%, affirmed by clinical efficacy.The present invention can adjust the compliance of patient's cardiac muscle, under the conditions of increasing myocardial anoxia Tolerance and myocardial ATP enzyme activity, reduce intra-atrial reentry, stablize myocardial cell membrane, improve cardiac muscle multipole conduction system Significant protective effect is presented to cardiac muscle in system function.The clinical efficacy for significantly improving patient improves the quality of life of patient, reaches To antiarrhythmic effect.From different perspectives, different levels, using modern science and technology means and method, with molecular biology Based on, a kind of mechanism of action of Chinese medicine composition that treating arrhythmia cordis is furtherd investigate and explored, is premature beat and room The drug therapy quivered provides objective basis, this has the more potential diagnosis of premature beat and atrial fibrillation and generation, the development of predictive disease Far-reaching application value.

Compared with prior art, trouble can be adjusted by having for a kind of Chinese medicine composition that treating arrhythmia cordis and quality determining method The compliance of person's cardiac muscle increases the activity of the tolerance and myocardial ATP enzyme under the conditions of myocardial anoxia, reduces intra-atrial reentry, surely Centering muscle cell membrane, improves the multipole conducting system function of cardiac muscle, significant protective effect is presented to cardiac muscle, hence it is evident that improve patient Clinical efficacy, improve the quality of life of patient, reach antiarrhythmic effect, from different perspectives, different levels, using the modern times Scientific and technological means and method are furtherd investigate and are explored to the mechanism of action of drug based on molecular biology, are early It fights and provides objective basis with the drug therapy of atrial fibrillation, this is to the potential diagnosis of premature beat and atrial fibrillation and generation, the development of predictive disease With more far-reaching application value, clinical observation proves that the present invention can improve and restore the overall efficiency of patient's ventricular late potential Up to 79.9%, the advantages that clinical efficacy is obtained certainly, will be widely used in technical field of Chinese medicine.

Description of the drawings

The following describes the present invention in detail with reference to the accompanying drawings and embodiments.

Fig. 1 be the present invention rat modeling before electrocardiogram.

Fig. 2 be the present invention rat modeling after instant electrocardiogram.

Fig. 3 be the present invention rat modeling after enter a group electrocardiogram.

Fig. 4 is each group rat heart muscle tissue Ca of the present invention²⁺- ATP mRNA express PCR product agarose gel electrophoresis knot Fruit is schemed.

Fig. 5 is each group rat heart muscle tissue Cx40 gene expression PCR product agarose gel electrophoresis result figures of the present invention.

Fig. 6 is the electrophoretogram one of the L-type calcium channel mRNA expression Long-term change trend of the present invention.

Fig. 7 is the electrophoretogram two of the L-type calcium channel mRNA expression Long-term change trend of the present invention.

Fig. 8 is the electrophoretogram one of extracellular regulated kinases 2 (ERK2) mRNA expression Long-term change trends of the present invention.

Fig. 9 is the electrophoretogram two of extracellular regulated kinases 2 (ERK2) mRNA expression Long-term change trends of the present invention.

Figure 10 be the present invention Electronic Speculum under each group rat heart muscle tissue hyper-microstructure situation normal control group picture one.

Figure 11 be the present invention Electronic Speculum under each group rat heart muscle tissue hyper-microstructure situation normal control group picture two.

Figure 12 be the present invention Electronic Speculum under each group rat heart muscle tissue hyper-microstructure situation model comparison group picture one.

Figure 13 be the present invention Electronic Speculum under each group rat heart muscle tissue hyper-microstructure situation model comparison group picture two.

Figure 14 be the present invention Electronic Speculum under each group rat heart muscle tissue hyper-microstructure situation Chinese herbs group figure one.

Figure 15 be the present invention Electronic Speculum under each group rat heart muscle tissue hyper-microstructure situation Chinese herbs group figure two.

Figure 16 be the present invention Electronic Speculum under each group rat heart muscle tissue hyper-microstructure situation western medicine group figure one.

Figure 17 be the present invention Electronic Speculum under each group rat heart muscle tissue hyper-microstructure situation western medicine group figure two.

Specific implementation mode

Reference attached drawing,

Embodiment one

(1) honey-fried licorice root 30g, Radix Rehmanniae 25g, ginseng 20g, Radix Astragali 30g are taken in prescription ratio, 70% second measured with 10 times Alcohol, twice, 2 hours every time, filtering, filtrate merged heating and refluxing extraction, and recycling ethyl alcohol obtains liquid I；

(2) Radix Ophiopogonis 25g, Radix Salviae Miltiorrhizae 35g, safflower 20g, spina date seed 20g are taken in prescription ratio, impregnated 30 minutes, by step (1) The middle gained dregs of a decoction are added, common decocting 2 times, for the first time 12 times of water, decoct 2 hours, and filtration obtains liquid II, second of 8 times of water, It decocts 1.5 hours, filtration obtains liquid III, collects distillate twice, obtains liquid IV；

(3) liquid I, II, III, IV is merged, mixing carries out coarse filtration and refined filtration, refined filtration liquid triple effect with plate and frame filter press It is 1.15 that energy-saving concentrator, which is concentrated into relative density, temperature 60 C；Concentrate spray drying process, feed speed 6000ml/ Min, centrifugal speed 6000r/min, 120 DEG C of inlet air temperature, prepare extract powder；20% dextrin mixing is added in cream powder, with 80% Ethanol wet is pelletized, wet granular boiled bed drying, 75 DEG C of wind-warm syndrome；Beta-cyclo dextrin included compound powder, whole grain is added；Inspection is sub-packed in In aluminium plastic membrane bag, 3g is often wrapped, both obtains finished dosage form, and pill, capsule, decoction, powder, electuary, granule or mouth is made Take liquid.

A kind of Chinese medicine composition that treating arrhythmia cordis and drug quality differentiate detection method, according to the animal of embodiment one Laboratory report：

Experimental method：Select the healthy male Wistar big white mouse 100, weight 250-300g of 10-12 week old, animal one As in good condition, fur gloss, feed, adaptable fed after a week modeling normal with activity.It is mixed using calcium chloride-acetylcholine Close the animal model that liquid establishes atrial fibrillation in such a way that caudal vein is administered.As stated above after modeling, screening has typical atrial fibrillation The rat of Electrocardiogram Feature 30, is randomly divided into, totally 3 groups, average every group of 10 rats.In addition no progress modeling processing is just 10 rats of normal control group are four groups altogether.Because there is rats death during medication, therefore every group of random selection 8 is only into row index Measurement.Each group animal is raised under equal conditions.Normal group, model group gavage the physiological saline of same isometric(al) once a day； By people's mouse dose lonvestion, dosage (g) × coefficient (0.081)/required the weight of animals of the people for dosage (g/Kg)=provide (Kg)。

Invigorating Qi Chinese herbs group：It gavages and is equivalent to 16.2g/kg/d crude drugs, once a day；Western medicine group fills Clothes are equivalent to the Verapamil of 4.5mg/kg/d crude drugs, once a day.Share medicine 4 weeks.Indices are measured after 4 weeks.It retouches respectively Note rat modeling before, after modeling, enter a group electrocardiogram after modeling；Observe the ordinary circumstance of rat：Including the state of mind, hair color, mouth Lip, tongue nature, breathing, the variation etc. of heart rate.The level of palatelet-selectin in serum is measured using double crush syndrome method.Using 14.0 software packages of SPSS are for statistical analysis.

(1) rat modeling electrocardiogram

Fig. 1 be the present invention rat modeling before electrocardiogram.Fig. 2 be the present invention rat modeling after instant electrocardiogram.Fig. 3 is Enter a group electrocardiogram after the rat modeling of the present invention.

(2) each group rat general state is observed

The visible hair color of normal rats is pure white, and fur Smooth and moist, expression is normal, and body movement is flexible, and breathing is steady, shape State is good.

The visible spirit of atrial fibrillation rat is not good enough after modeling, and happiness is tired sleeping, and respiratory rate is accelerated, and claw has livid purple color, skin with lip Hair is not so good as preoperative Smooth and moist, and breathing, heart rate number are obviously accelerated.After the four weeks of administration, Chinese medicine group, Western medicine Verapamil pair It is clearly better according to the rat state of group, appetite increases, and activity is flexibly；Model group state is not good enough earlier above.Prompt Dingxin capsule group and Verapamil group all has an impact the observation index of AF rats.Because there is rats death during medication, therefore every group of random selection 8 Only into the measurement of row index.

(3) palatelet-selectin situation of change in each group rat blood serum, is shown in Table 4.

Palatelet-selectin situation of change in 4 each group rat blood serum of table

Group	Number of cases	Palatelet-selectin (ng/ml)
			Blank control group	8	51.186±9.571
Model control group	8	122.836±12.998*
			Chinese medicine group	8	92.289±16.121^Δ#
Western medicine group	8	98.34±13.451^Δ

Note：Compared with blank group, * P<0.01；Compared with model group,^ΔP<0.01；Compared with Verapamil group,^#P> 0.05。

It can be seen that palatelet-selectin, in model group and the more significant difference of blank control group, P ＜ 0.01 illustrate that model group is big Palatelet-selectin content is significantly larger than normal group in mouse serum, prompts when atrial fibrillation that palatelet-selectin content increases in blood plasma, and blood platelet is lived Change；Palatelet-selectin content is substantially reduced treatment group's (including Western medicine, Chinese medicine group) compared with model group, P ＜ 0.01, illustrates have significantly Meaning, treatment are effective.Chinese medicine group P compared with western medicine group>0.05, it is not statistically significant, illustrates Chinese medicine and Western medicine Wella Pa rice is similar with prevention effect to the treatment of atrial fibrillation.

Embodiment two

(1) honey-fried licorice root 30g, Radix Rehmanniae 25g, ginseng 25g, Radix Astragali 25g are taken in prescription ratio, 70% second measured with 10 times Alcohol, twice, 2 hours every time, filtering, filtrate merged heating and refluxing extraction, and recycling ethyl alcohol obtains liquid I；

(2) Radix Ophiopogonis 25g, Radix Salviae Miltiorrhizae 35g, safflower 20g, spina date seed 25g are taken in prescription ratio, impregnated 30 minutes, by step (1) The middle gained dregs of a decoction are added, common decocting 2 times, for the first time 12 times of water, decoct 2 hours, and filtration obtains liquid II, second of 8 times of water, It decocts 1.5 hours, filtration obtains liquid III, collects distillate twice, obtains liquid IV；

A kind of Chinese medicine composition that treating arrhythmia cordis and drug quality differentiate detection method, according to the animal of embodiment two Laboratory report：

Experimental method：Select the healthy male Wistar big white mouse 100, weight 250-300g of 10-12 week old, animal one As in good condition, fur gloss, feed, adaptable fed after a week modeling normal with activity.It is mixed using calcium chloride-acetylcholine Close the animal model that liquid establishes atrial fibrillation in such a way that caudal vein is administered.As stated above after modeling, screening has typical atrial fibrillation The rat of Electrocardiogram Feature 30, is randomly divided into, totally 3 groups, average every group of 10 rats.In addition no progress modeling processing is just 10 rats of normal control group are four groups altogether.Because there is rats death during medication, therefore every group of random selection 8 is only into row index Measurement.Each group animal is raised under equal conditions.Normal group, model group gavage the physiological saline of same isometric(al) once a day； By people's mouse dose lonvestion, dosage (g) × coefficient (0.081)/required the weight of animals of the people for dosage (g/Kg)=provide (Kg).Invigorating Qi Chinese herbs group：It gavages and is equivalent to 16.2g/kg/d crude drugs, once a day；Western medicine group gavages It is equivalent to the Verapamil of 4.5mg/kg/d crude drugs, once a day.Share medicine 4 weeks.After 4 weeks blood is measured using radioimmunology method Thromboxane B in clear₂(TXB₂) level.It is for statistical analysis using SPSS14.0 software packages.

TXB in 5 each group rat blood serum of table₂The case where

Group	Number of cases	TXB₂(X±S)
			Normal group	8	36.39±4.94
Model group	8	129.57±16.29*
			Chinese medicine group	8	44.10±4.68**
Western medicine group	8	70.78±9.56#

* the P ＜ 0.05 compared with normal group, * * the P ＜ 0.05 compared with model group, # the P ＜ 0.05 compared with Chinese medicine group, with mould Type group compares P ＜ 0.05.

From table 5：Model group has significant difference (P ＜ 0.01), illustrates model group rats serum compared with normal group Middle TXB₂It more normally organizes statistically significant.Chinese medicine group and Western medicine group are compared with model group, and P ＜ 0.05 are statistically significant；But Chinese medicine group and Western medicine group compare, and Chinese medicine group is better than Western medicine group (P ＜ 0.05).In this experiment, TXB in the serum of model group₂It is high It is in normal group and statistically significant, when illustrating atrial fibrillation, TXB in serum₂Level increases, and Chinese medicine can actually lower serum Middle TXB₂Level, to mitigate the breaking-out of AF.

In 6 each group rat blood serum of table the case where PAI-I

Group	Number of cases	PAⅠ-1(X±S)
			Normal group	8	16.09±5.41
Model group	8	49.33±9.10*
			Chinese medicine group	8	19.40±5.14**
Western medicine group	8	29.61±4.91#

From table 6：Model group has significant difference (P ＜ 0.01), illustrates model group rats serum compared with normal group Middle PA I -1 more normally organizes statistically significant.Chinese medicine group and Western medicine group are compared with model group, and P ＜ 0.05 are statistically significant, And Chinese medicine group and Western medicine group compare, Chinese medicine group is better than Western medicine group (P ＜ 0.05).In this experiment, PAI-I in the serum of model group Higher than normal group and statistically significant, when illustrating atrial fibrillation, PAI-I levels increase in serum, and invigorating Qi Chinese medicine Dingxin capsule can actually lower PAI-I levels in serum, to mitigate the breaking-out of AF.

Embodiment three

(1) honey-fried licorice root 25g, Radix Rehmanniae 30g, ginseng 20g, Radix Astragali 35g are taken in prescription ratio, 70% second measured with 10 times Alcohol, twice, 2 hours every time, filtering, filtrate merged heating and refluxing extraction, and recycling ethyl alcohol obtains liquid I；

(2) Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 35g, safflower 20g, spina date seed 20g are taken in prescription ratio, impregnated 30 minutes, by step (1) The middle gained dregs of a decoction are added, common decocting 2 times, for the first time 12 times of water, decoct 2 hours, and filtration obtains liquid II, second of 8 times of water, It decocts 1.5 hours, filtration obtains liquid III, collects distillate twice, obtains liquid IV；

A kind of Chinese medicine composition that treating arrhythmia cordis and drug quality differentiate detection method, according to the animal of embodiment three Laboratory report：

Experimental method：Select the healthy male Wistar big white mouse 100, weight 250-300g of 10-12 week old, animal one As in good condition, fur gloss, feed, adaptable fed after a week modeling normal with activity.It is mixed using calcium chloride-acetylcholine Close the animal model that liquid establishes atrial fibrillation in such a way that caudal vein is administered.As stated above after modeling, screening has typical atrial fibrillation The rat of Electrocardiogram Feature 30, is randomly divided into, totally 3 groups, average every group of 10 rats.In addition no progress modeling processing is just 10 rats of normal control group are four groups altogether.Because there is rats death during medication, therefore every group of random selection 8 is only into row index Measurement.Each group animal is raised under equal conditions.Normal group, model group gavage the physiological saline of same isometric(al) once a day； By people's mouse dose lonvestion, dosage (g) × coefficient (0.081)/required the weight of animals of the people for dosage (g/Kg)=provide (Kg).Invigorating Qi Chinese herbs group：It gavages and is equivalent to 16.2g/kg/d crude drugs, once a day；Western medicine group fills Clothes are equivalent to the Verapamil of 4.5mg/kg/d crude drugs, once a day.Share medicine 4 weeks.It is put to death greatly under narcosis after 4 weeks Mouse, be stored in after taking left ventricle to be freezed in liquid nitrogen -70 DEG C it is spare.RT-PCR method measures cardiac muscular tissue Ca²⁺ATP enzyme gene Expression.It is for statistical analysis using 14.0 software packages of SPSS.

Each group rat heart muscle tissue Ca²⁺The situation of change of ATP enzyme gene expression：

Ca is measured with RT-PCR method²⁺- ATP mRNA and β-actin expression, and agarose is carried out to RT-PCR products Gel electrophoresis produces two light belts, such as Fig. 1：The above is Ca²⁺- ATP mRNA expressions, and it is below β-actin expression Situation, then calculate Ca²⁺The gray density ratio of-ATP mRNA/ β-actin carries out comparison among groups, as shown in table 7.

Fig. 4 is each group rat heart muscle tissue Ca²⁺- ATP mRNA express PCR product agarose gel electrophoresis result figure.Fig. 4 In 1 be Marka；2,3 be normal group in Fig. 4；4,5 be model group in Fig. 4；6,7 be Chinese medicine group in Fig. 4；8,9 be Western medicine in Fig. 4 Group.

7 each group rat heart muscle tissue Ca of table²⁺ATP enzyme gene/β-actin expressions

From table 7：Model group is compared with normal group, and (P ＜ 0.05=, illustrate model group rats Ca to significant difference² ⁺ATP enzyme gene expression is more normally organized statistically significant.Chinese medicine group and Western medicine group are compared with model group, P ＜ 0.05, have significantly Statistical significance；But Chinese medicine group and Western medicine group compare, and Chinese medicine group is better than Western medicine group (P ＜ 0.05=.In this experiment, model group Ca²⁺ATP enzyme gene expression is higher than normal group and statistically significant, when illustrating atrial fibrillation, the Ca of cardiac muscular tissue²⁺ATP enzyme Increase or density increases, and invigorating Qi Chinese medicine can actually lower Ca²⁺The mRNA of ATP enzyme is expressed, to mitigate AF's Breaking-out.

Example IV

(1) honey-fried licorice root 25g, Radix Rehmanniae 25g, ginseng 20g, Radix Astragali 30g are taken in prescription ratio, 70% second measured with 10 times Alcohol, twice, 2 hours every time, filtering, filtrate merged heating and refluxing extraction, and recycling ethyl alcohol obtains liquid I；

(2) Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 40g, safflower 20g, spina date seed 25g are taken in prescription ratio, impregnated 30 minutes, by step (1) The middle gained dregs of a decoction are added, common decocting 2 times, for the first time 12 times of water, decoct 2 hours, and filtration obtains liquid II, second of 8 times of water, It decocts 1.5 hours, filtration obtains liquid III, collects distillate twice, obtains liquid IV；

A kind of Chinese medicine composition that treating arrhythmia cordis and drug quality differentiate detection method, according to the animal of example IV Laboratory report：

Experimental method：Select the healthy male Wistar big white mouse 100, weight 250-300g of 10-12 week old, animal one As in good condition, fur gloss, feed, adaptable fed after a week modeling normal with activity.It is mixed using calcium chloride-acetylcholine Close the animal model that liquid establishes atrial fibrillation in such a way that caudal vein is administered.As stated above after modeling, screening has typical atrial fibrillation The rat of Electrocardiogram Feature 30, is randomly divided into 3 groups, average every group of 10 rats.In addition no carry out the normal of modeling processing 10 rats of control group are four groups altogether.Because there is rats death during medication, therefore every group of random selection 8 is only into row index It measures.Each group animal is raised under equal conditions.Normal group, model group gavage the physiological saline of same isometric(al) once a day；It presses People's mouse dose lonvestion, dosage (g) × coefficient (0.081)/required the weight of animals (Kg) of the people for dosage (g/Kg)=provide. Invigorating Qi Chinese herbs group：It gavages and is equivalent to 16.2g/kg/d crude drugs, once a day；Western medicine group gavages quite In the Verapamil of 4.5mg/kg/d crude drugs, once a day.Share medicine 4 weeks.Rat is put to death under narcosis after 4 weeks, takes a left side Ventricle be stored in after being freezed in liquid nitrogen -70 DEG C it is spare.RT-PCR method measures cardiac muscular tissue's Cx40 gene expressions.Using 14.0 software packages of SPSS are for statistical analysis.

The situation of change of each group rat heart muscle tissue Cx40 gene expressions：

Cx40 gene expressions and β-actin expression are measured with RT-PCR method, and agarose is carried out to RT-PCR products Gel electrophoresis produces two light belts, such as Fig. 2：The above is Cx40 expression conditions, and is below β-actin expressions, The gray density ratio for calculating Cx40mRNA/ β-actin again carries out comparison among groups, as shown in table 8.

Fig. 5 is each group rat heart muscle tissue Cx40 gene expression PCR product agarose gel electrophoresis result figures of the present invention. 1 is Marka in Fig. 5；2,3 be normal group in Fig. 5；4,5 be model group in Fig. 5；6,7 be Chinese medicine group in Fig. 5；8,9 be west in Fig. 5 Medicine group.

8 each group rat heart muscle tissue Cx40 genes of table/β-actin expressions

Group	Number of cases	Intensity ratio
			Normal group	8	0.31±5.88×10^-2
Model group	8	2.08±42.66×10^-2*
			Chinese medicine group	8	0.92±11.7×10^-2**
Western medicine group	8	1.16±8.21×10^-2#

From table 8：Model group is compared with normal group, and (P ＜ 0.05=, illustrate model group rats to significant difference Cx40 gene expressions are more normally organized statistically significant.Compared with model group, P ＜ 0.05 have statistics for Chinese medicine group and Western medicine group Meaning；But Chinese medicine group and Western medicine group compare, and Chinese medicine group is better than Western medicine group (P ＜ 0.05=.In this experiment, the Cx40 of model group Gene expression is higher than normal group and statistically significant, when illustrating atrial fibrillation, the Cx40 gene expressions of cardiac muscular tissue increase or Density increases, and invigorating Qi Chinese medicine can actually lower Cx40 gene expressions, to mitigate the breaking-out of AF.

Embodiment five

(2) Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 35g, safflower 25g, spina date seed 25g are taken in prescription ratio, impregnated 30 minutes, by step (1) The middle gained dregs of a decoction are added, common decocting 2 times, for the first time 12 times of water, decoct 2 hours, and filtration obtains liquid II, second of 8 times of water, It decocts 1.5 hours, filtration obtains liquid III, collects distillate twice, obtains liquid IV；

A kind of Chinese medicine composition that treating arrhythmia cordis and drug quality differentiate detection method, according to the animal of embodiment five Laboratory report：

Experimental method：Select the healthy male Wistar big white mouse 100, weight 250-300g of 10-12 week old, animal one As in good condition, fur gloss, feed, adaptable fed after a week modeling normal with activity.It is mixed using calcium chloride-acetylcholine Close the animal model that liquid establishes atrial fibrillation in such a way that caudal vein is administered.As stated above after modeling, screening has typical atrial fibrillation The rat of Electrocardiogram Feature 30, is randomly divided into, totally 3 groups, average every group of 10 rats.In addition no progress modeling processing is just 10 rats of normal control group are four groups altogether.Because there is rats death during medication, therefore every group of random selection 8 is only into row index Measurement.Each group animal is raised under equal conditions.Normal group, model group gavage the physiological saline of same isometric(al) once a day； By people's mouse dose lonvestion, dosage (g) × coefficient (0.081)/required the weight of animals of the people for dosage (g/Kg)=provide (Kg).Invigorating Qi Chinese herbs group：It gavages and is equivalent to 16.2g/kg/d crude drugs, once a day；Western medicine group gavages It is equivalent to the Verapamil of 4.5mg/kg/d crude drugs, once a day.Share medicine 4 weeks.Rat is put to death under narcosis after 4 weeks, Be stored in after taking left ventricle to be freezed in liquid nitrogen -70 DEG C it is spare.RT-PCR method measures cardiac muscular tissue's L-type calcium channel gene table It reaches and extracellular regulated kinases 2 (EPK2) gene expression.For statistical analysis using 14.0 software packages of SPSS, comparison among groups are used Variance analysis, if heterogeneity of variance is examined using Dunnett.

(1) influence that invigorating Qi Chinese medicine expresses experimental AF rat heart muscles ERK2, LTCC mRNA

The influence (X scholar s) that invigorating Qi Chinese medicine expresses experimental AF rat heart muscles ERK2, LTCC mRNA

The influence (X scholar s) that 9 invigorating Qi Chinese medicine of table expresses experimental AF rat heart muscles ERK2, LTCC mRNA

Group	ERK2mRNA/β-actin mRNA	LTCCmRNA/β-actin mRNA
			Blank control group	0.23458±0.03908	0.80578±0.05135
Model control group	0.51158±0.05408*	0.31592±0.04408*
			Dingxin capsule group	0.26824±0.02688^Δ#	0.70348±0.03878^Δ#
Verapamil group	0.49709±0.04740	0.61867±0.03098^Δ

Note：Compared with blank group, * P<0.05；Compared with model group,^ΔP<0.05；Compared with Verapamil group,^#P< 0.05。

(2) L-type calcium channel mRNA expression Long-term change trend

Model control group rat reduces compared with blank control group L-type calcium channel mRNA expression level, and two groups are compared with notable Sex differernce (P<0.05).It is invigorating Qi Chinese herbs group, Verapamil intervention group, apparent compared with model control group Improve the state (P that L-type calcium channel mRNA expression level reduces in atrial fibrillation rat atrial tissue<0.05), dry with Verapamil Pre- group compares, and invigorating Qi Chinese herbs group curative effect is better than Verapamil intervention group (P<0.05).

Fig. 6 is the electrophoretogram one of the L-type calcium channel mRNA expression Long-term change trend of the present invention.1,2 be normal group in Fig. 6；Figure 3,4 be model group in 6；5,6 be Chinese medicine group in Fig. 6；7,8 be Western medicine group in Fig. 6.

Fig. 7 is the electrophoretogram two of the L-type calcium channel mRNA expression Long-term change trend of the present invention.1,2 be normal group in Fig. 7；Figure 3,4 be model group in 7；5,6 be Chinese medicine group in Fig. 7；7,8 be Western medicine group in Fig. 7.

(3) extracellular regulated kinases 2 (ERK2) mRNA expresses Long-term change trend

Expression of the model control group rat compared with blank control group extracellular regulated kinases 2 (ERK2) mRNA increases, in The expression of medicine treatment group extracellular regulated kinases 2 (ERK2) mRNA reduces, hence it is evident that improves in atrial fibrillation rat atrial tissue Raised state (the P of expression of extracellular regulated kinases 2 (ERK2) mRNA<0.05), Verapamil intervention group is to extracellular The raised state of expression for adjusting kinases 2 (ERK2) mRNA is not improved.

Fig. 8 is the electrophoretogram one of extracellular regulated kinases 2 (ERK2) mRNA expression Long-term change trends of the present invention.1,2 is just Normal group, 3,4 be model group, and 5,6 be Chinese medicine group, and 7,8 be Western medicine group.

Fig. 9 is the electrophoretogram two of extracellular regulated kinases 2 (ERK2) mRNA expression Long-term change trends of the present invention.1,2 is just Normal group, 3,4 be model group, and 5,6 be Chinese medicine group, and 7,8 be Western medicine group.

Embodiment six

(1) honey-fried licorice root 40g, Radix Rehmanniae 25g, ginseng 25g, Radix Astragali 30g are taken in prescription ratio, 70% second measured with 10 times Alcohol, twice, 2 hours every time, filtering, filtrate merged heating and refluxing extraction, and recycling ethyl alcohol obtains liquid I；

A kind of Chinese medicine composition that treating arrhythmia cordis and drug quality differentiate detection method, according to the animal of embodiment six Laboratory report：

Experimental method：Select the healthy male Wistar big white mouse 100, weight 250-300g of 10-12 week old, animal one As in good condition, fur gloss, feed, adaptable fed after a week modeling normal with activity.It is mixed using calcium chloride-acetylcholine Close the animal model that liquid establishes atrial fibrillation in such a way that caudal vein is administered.As stated above after modeling, screening has typical atrial fibrillation The rat of Electrocardiogram Feature 30, is randomly divided into, totally 3 groups, average every group of 10 rats.In addition no progress modeling processing is just 10 rats of normal control group are four groups altogether.Because there is rats death during medication, therefore every group of random selection 8 is only into row index Measurement.Each group animal is raised under equal conditions.Normal group, model group gavage the physiological saline of same isometric(al) once a day； By people's mouse dose lonvestion, dosage (g) × coefficient (0.081)/required the weight of animals of the people for dosage (g/Kg)=provide (Kg).Chinese herbs group：It gavages and is equivalent to 16.2g/kg/d crude drugs, once a day；Western medicine group, which gavages, is equivalent to 4.5mg/ The Verapamil of kg/d crude drugs, once a day.Share medicine 4 weeks.Rat is put to death under narcosis after 4 weeks, takes left ventricle in liquid In nitrogen freeze after be stored in -70 DEG C it is spare.Rat atrial is taken to be cut into 1mm³Several pieces of size cleans blood with ice physiological saline, Again with 2.5% glutaraldehyde, 1% osmic acid OsO₄Dual fixation, alcohol, acetone serial dehydration, epoxy resin Epon812 embeddings, system At ultra-thin section, the double dyeing of uranium, lead are used later, with the ultra microstructure of transmission electron microscope observing cardiac muscular tissue.

Each group rat heart muscle tissue hyper-microstructure situation is as follows under Electronic Speculum：(see attached drawing 10-17)

Normal group (Figure 10,11)：Visible cardiac muscle cell's band is clear under Electronic Speculum, and muscle corpuscle is located under muscle fibril Side, sarcoplasm mitochondrial quantity is more, the uniform no swelling of size and form, and ridge arrangement is close, and muscle fibril traveling is neat, adjacent flesh There are intercalated disc, intercalated disc to be located at the both ends of cell between fiber.

Model group (Figure 12,13)：It can be seen that Myocardial ultrastructure has major injury, cardiac muscle cell's mitochondrial swelling, ridge Fracture is in vacuole sample, and karyopyknosis is in irregular type, and electron density reduces, and metachromatin accumulates in the periphery of core in core, it is seen that Kernel, muscle fibril is disorganized, muscle segment is broken, and intercalated disc is discontinuous, existing rough surfaced endoplasmic reticulum (RER) in sarcoplasmic reticulum, it is seen that glycogen particle, Fat drips etc..

The visible cardiac muscle cell's muscle fibril of Chinese herbs group (Figure 14,15) is normal, arrangement is close, and muscle corpuscle is normal, line Plastochondria volume becomes smaller, and shape owes rule, and ridge is intensive, quantity is more, relatively with normal group.

The visible cardiac muscle cell's muscle fibril of western medicine group (Figure 16,17) is close to normally, and arrangement is closer, and muscle corpuscle connects Close normal, mitochondria volume becomes smaller, and shape is irregular, and no fiber is disorderly, is broken and lacks, and is closer to normal group.

Embodiment seven

(2) Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 35g, safflower 25g, spina date seed 40g are taken in prescription ratio, impregnated 30 minutes, by step (1) The middle gained dregs of a decoction are added, common decocting 2 times, for the first time 12 times of water, decoct 2 hours, and filtration obtains liquid II, second of 8 times of water, It decocts 1.5 hours, filtration obtains liquid III, collects distillate twice, obtains liquid IV；

A kind of Chinese medicine composition that treating arrhythmia cordis and drug quality differentiate detection method, according to the animal of embodiment seven Laboratory report：

Experimental method：Select the healthy male Wistar big white mouse 100, weight 250-300g of 10-12 week old, animal one As in good condition, fur gloss, feed, adaptable fed after a week modeling normal with activity.It is mixed using calcium chloride-acetylcholine Close the animal model that liquid establishes atrial fibrillation in such a way that caudal vein is administered.As stated above after modeling, screening has typical atrial fibrillation The rat of Electrocardiogram Feature 30, is randomly divided into, totally 3 groups, average every group of 10 rats.In addition no progress modeling processing is just 10 rats of normal control group are four groups altogether.Because there is rats death during medication, therefore every group of random selection 8 is only into row index Measurement.Each group animal is raised under equal conditions.Normal group, model group gavage the physiological saline of same isometric(al) once a day； By people's mouse dose lonvestion, dosage (g) × coefficient (0.081)/required the weight of animals of the people for dosage (g/Kg)=provide (Kg).Chinese herbs group：It gavages and is equivalent to 16.2g/kg/d crude drugs, once a day；Western medicine group, which gavages, is equivalent to 4.5mg/ The Verapamil of kg/d crude drugs, once a day.Share medicine 4 weeks.Rat blood serum is measured using double-antibody sandwich ABC~ELISA method The level of middle MMP-9.Anti- rat MMP-9 monoclonal antibodies are coated on ELISA Plate, and the MMP-9 in standard items and sample is combined with monoclonal antibody, Biotinylated anti-rat MMP-9 is added, forms immune complex connection onboard, the Strep of horseradish peroxidase-labeled Ta-vidin is combined with biotin, and zymolyte OPO is added, yellow occurs, adds terminate liquid, after darkening, is surveyed at 492nm OD values, MMP-9 concentration is directly proportional to OD values, and the concentration of sample MMP-9 is found out by drawing standard curve.

The variation (X scholar s) of MMP-9 in 10 each group rat blood serum of table

Group	Number of cases	MMP-9(X±S)
			Normal group	8	1.840±0.280
Model group	8	4.060±1.040**
			Chinese medicine group	8	2.325±1.450△
Western medicine group	8	3.245±1.635#*

Note：Compared with normal group, * P ＜ 0.05；Compared with model group, △ P ＜ 0.05；Compared with Chinese medicine group, #P ＜ 0.05

One, instrument and reagent

LC-10P liquid phase pump LC-10UV UV detector, big Liaanjiang county Shen separation science Technology Co., Ltd. production；Chromatographic column 1 (Hypersil), Dalian Yi Lite Co., Ltds provide；5 μ, 4.6mm × 200mm；Chromatographic column 2 (Spherigel), big Liaanjiang county Shen separation science Co., Ltd provides；5 μ, 4.6mm × 200mm；Agents useful for same acetonitrile is chromatographically pure, and Shandong king's Yu industry is limited Company Yucheng chemical plant；Water is double distilled water, and other reagents are that analysis is pure；Ginsenoside Rg1's reference substance, Chinese drug biology system Product examine is fixed to be provided, and assay is used, lot number 0703-9812；

Two, method and result

1, thin layer differentiates

(1) thin layer of Radix Salviae Miltiorrhizae differentiates

(2) thin layer of Radix Astragali differentiates

(3) thin layer of spina date seed differentiates

2, assay

(1) chromatographic condition：

(2) preparation of reference substance solution:

(3) preparation of test solution：

(4) selection of Detection wavelength：

Calibration curve equation is Y=9.163 × 10^-8X+1.074×10^-2R=0.9997；

Sample introduction is ranging from：2.156 μ of μ g~10.078 g；

(6) precision test：

(7) blank test：

(8) stability test：

(9) reappearance test：

(10) sample recovery rate is tested：

(11) Determination of Content of Ginsenoside Rg_1 measures in sample:

Three, conclusion

1, pharmacodynamic action

2, detection method result

A kind of Chinese medicine composition that treating arrhythmia cordis and drug quality differentiate the clinical experiment report of detection method：

1, clinical experiment report one

(1) pharmaceutical formulation that clinical test uses

Honey-fried licorice root 35g, Radix Rehmanniae 30g, ginseng 25g, Radix Astragali 25g, Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 35g, safflower 20g, spina date seed 30g.

(2) clinical experimental study method：Selection of clinical ventricular late potential exception and TCM Syndrome Type category deficiency of both qi and yin syndrome of blood stasis Person 30, wherein male 21, women 9；The oldest person 64 years old, reckling 42 years old are 56 years old average；Course of disease longest 3 months, It is most 2 weeks short；Protopathy is coronary heart disease 9, outmoded 18 Myocardial Infarction Cases, cardiomyopathy 3, companion's cardiac insufficiency person 8.All diseases Example has different degrees of ventricular premature beat, wherein 3 with atrial fibrillation.All cases while treating underlying diseases, are given Give Chinese medicine treatment.Daily 1 dose, decocting divides 3 times and takes orally, and 1 month as a treatment course.Curative effect determinate standard：Ventricular late potential feminine gender is It cures, it is effective that above-mentioned diagnostic criteria, which improves 1/2ms, and it is invalid not change into.Statistical is carried out using 14.0 software packages of SPSS Analysis.As a result as follows:

11 clinical experimental study result of table

Chinese medicine fixed core square group	Ventricular late potential	Diagnostic criteria	Diagnostic criteria	Total effective rate
						It is negative	Improve 1/2ms	Without improvement
30	19	5	6	79.9%
					Curative effect rate %	63.3	16.6	20.1

(3) clinical test results：

Show that fixed core square improves and the overall efficiency of recovery ventricular late potential is up to 79.9% by clinical observation, faces Bed curative effect is affirmed, to provide new idea and method extremely for treatment ventricular late potential.

2, clinical experiment report two

(1) pharmaceutical formulation that clinical test uses

Honey-fried licorice root 35g, Radix Rehmanniae 30g, ginseng 20g, Radix Astragali 25g, Radix Ophiopogonis 25g, Radix Salviae Miltiorrhizae 40g, safflower 20g, spina date seed 40g.

(2) clinical experimental study method：Selection of clinical is clarified a diagnosis as by protopathy, coronary heart disease, high worry, the wind heart The atrial fibrillation that disease, myocarditis, cardiomyopathy, pulmonary heart disease cause, and TCM Syndrome Type category deficiency of both qi and yin syndrome of blood stasis person, totally 60.Its Middle male 28, women 32；Oldest 75 years old, minimum 29 years old.The course of disease is all at 1 year or more；Protopathy, coronary heart disease 13, high worry 10, rheumatic heart disease 10, myocarditis 9, cardiomyopathy 8, pulmonary heart disease 5, unknown 5 of reason.All diseases Example while treating underlying diseases, gives Chinese medicine treatment.One dose per day takes orally in three times, and 30 doses as a treatment course, generally A course for the treatment of is taken, single cases can take two courses for the treatment of according to my situation.We are just included in statistics after one course for the treatment of Object, observe Chinese medicine the effect of situation.All antiarrhythmic drugs are deactivated during taking Chinese medicine.With the disease of observation post administration patient Shape improves situation.Curative effect determinate standard：(1) effective：Atrial attack number reduces 50%, and ventricular rate slows down 1/3, and symptom is apparent Improvement is effective.(2) effectively：Atrial attack number significantly reduces, and ventricular rate part slows down, and it is to have that symptom part, which improves, Effect.(3) invalid：Atrial attack number does not change, and it is invalid that symptom, which does not improve,.

12 clinical experimental study result of table

Chinese medicine fixed core square group	It is effective	Effectively	In vain	It is efficient
					30	19	32	9	85%
Cardinal symptom	Palpitaition, shortness of breath uncomfortable in chest	Insomnia, dreaminess	Dizzy, burnout	Tongue vein improves
					Symptom improvement rate %	85	60	55	46

(3) clinical test results：

Clinical observation shows atrial fibrillation deficiency of both qi and yin and syndrome of blood stasis on the basis of conventional therapy plus in invigorating Qi Medicine can significantly improve the clinical efficacy of patient, improve the quality of life of patient.

Claims

1. a kind of Chinese medicine composition for treating arrhythmia cordis, including Chinese medicine major ingredient and pharmaceutic adjuvant, it is characterised in that：In described Medicine major ingredient is made of following ingredients weight parts proportioning Chinese medicine：Honey-fried licorice root 25-40g, Radix Rehmanniae 25-30g, ginseng 20-25g, Radix Astragali 25-40g, Radix Ophiopogonis 25-40g, Radix Salviae Miltiorrhizae 35-40g, safflower 20-25g, spina date seed 20-40g.

2. a kind of Chinese medicine composition for treating arrhythmia cordis according to claim 1, it is characterised in that：The Chinese medicine master Material is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 30g, Radix Rehmanniae 25g, ginseng 20g, Radix Astragali 30g, Radix Ophiopogonis 25g, Radix Salviae Miltiorrhizae 35g, safflower 20g, spina date seed 20g.

3. a kind of Chinese medicine composition for treating arrhythmia cordis according to claim 1, it is characterised in that：The Chinese medicine master Material is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 30g, Radix Rehmanniae 25g, ginseng 25g, Radix Astragali 25g, Radix Ophiopogonis 25g, Radix Salviae Miltiorrhizae 35g, safflower 20g, spina date seed 25g.

4. a kind of Chinese medicine composition for treating arrhythmia cordis according to claim 1, it is characterised in that：The Chinese medicine master Material is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 25g, Radix Rehmanniae 30g, ginseng 20g, Radix Astragali 35g, Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 35g, safflower 20g, spina date seed 20g.

5. a kind of Chinese medicine composition for treating arrhythmia cordis according to claim 1, it is characterised in that：The Chinese medicine master Material is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 25g, Radix Rehmanniae 25g, ginseng 20g, Radix Astragali 30g, Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 40g, safflower 20g, spina date seed 25g.

6. a kind of Chinese medicine composition for treating arrhythmia cordis according to claim 1, it is characterised in that：The Chinese medicine master Material is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 30g, Radix Rehmanniae 25g, ginseng 20g, Radix Astragali 30g, Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 35g, safflower 25g, spina date seed 25g.

7. a kind of Chinese medicine composition for treating arrhythmia cordis according to claim 1, it is characterised in that：The Chinese medicine master Material is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 40g, Radix Rehmanniae 25g, ginseng 25g, Radix Astragali 30g, Radix Ophiopogonis 25g, Radix Salviae Miltiorrhizae 35g, safflower 20g, spina date seed 25g.

8. a kind of Chinese medicine composition for treating arrhythmia cordis according to claim 1, it is characterised in that：The Chinese medicine master Material is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 25g, Radix Rehmanniae 25g, ginseng 20g, Radix Astragali 30g, Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 35g, safflower 25g, spina date seed 40g.

9. a kind of Chinese medicine composition for treating arrhythmia cordis according to claim 1, it is characterised in that：The Chinese medicine master Material is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 35g, Radix Rehmanniae 30g, ginseng 25g, Radix Astragali 25g, Radix Ophiopogonis 30g, Radix Salviae Miltiorrhizae 35g, safflower 20g, spina date seed 30g.

10. a kind of Chinese medicine composition for treating arrhythmia cordis according to claim 1, it is characterised in that：The Chinese medicine Major ingredient is made of following ingredients weight parts optimum ratio Chinese medicine：Honey-fried licorice root 35g, Radix Rehmanniae 30g, ginseng 20g, Radix Astragali 25g, Radix Ophiopogonis 25g, Radix Salviae Miltiorrhizae 40g, safflower 20g, spina date seed 40g.

11. a kind of Chinese medicine composition for treating arrhythmia cordis according to claim 1, it is characterised in that：Described is medicinal Auxiliary material is one or more of in dextrin, starch, lactose.

12. a kind of preparation method of Chinese medicine composition for treating arrhythmia cordis according to claim 1, it is characterised in that： A kind of preparation method of Chinese medicine composition for treating arrhythmia cordis includes the following steps：

(1) honey-fried licorice root, Radix Rehmanniae, ginseng, Radix Astragali are taken in prescription ratio, 70% ethyl alcohol measured with 10 times, heating and refluxing extraction two Secondary, 2 hours every time, filtering, filtrate merged, and recycling ethyl alcohol obtains liquid I；

(2) Radix Ophiopogonis, Radix Salviae Miltiorrhizae, safflower, spina date seed are taken in prescription ratio, impregnated 30 minutes, the gained dregs of a decoction in step (1) are added, Common decocting 2 times, 12 times of water, decoct 2 hours for the first time, and filtration obtains liquid II, and second of 8 times of water decocts 1.5 hours, filter It crosses, obtains liquid III, collect distillate twice, obtain liquid IV；

(3) liquid I, II, III, IV is merged, mixing carries out coarse filtration and refined filtration, refined filtration liquid Three-effect energy-saving with plate and frame filter press It is 1.15 that inspissator, which is concentrated into relative density, temperature 60 C；Concentrate spray drying process, 6000 ml/min of feed speed, 6000 revs/min of centrifugal speed, 120 DEG C of inlet air temperature, prepares extract powder；20% dextrin mixing is added in cream powder, with 80% second Alcohol wetting granulation, wet granular boiled bed drying, 75 DEG C of wind-warm syndrome；Beta-cyclo dextrin included compound powder, whole grain is added；Inspection is sub-packed in aluminium It moulds in bag film, often wraps 3 grams, both obtain finished dosage form.

13. a kind of preparation method of Chinese medicine composition for treating arrhythmia cordis according to claim 1 or 12, feature exist In：Preparation in the step (3) is pill, capsule, decoction, powder, electuary, particle or oral solution.

14. a kind of quality determining method of Chinese medicine composition for treating arrhythmia cordis according to claim 1, feature exist In：A kind of quality determining method of Chinese medicine composition for treating arrhythmia cordis is as follows：

One, instrument and reagent

LC-10P liquid phase pump LC-10UV UV detector, big Liaanjiang county Shen separation science Technology Co., Ltd. production；Chromatographic column 1 (Hypersil)：Dalian Yi Lite Co., Ltds provide；5 μ, 4.6mm × 200mm；Chromatographic column 2 (Spherigel)：Big Liaanjiang county Shen Separation science Co., Ltd provides；5 μ, 4.6mm × 200mm；Agents useful for same acetonitrile is chromatographically pure, the limited public affairs of Shandong king's Yu industry Take charge of Yucheng chemical plant；Water is double distilled water, and other reagents are that analysis is pure；Ginsenoside Rg1's reference substance, Chinese pharmaceutical biological product Calibrating is provided, and assay is used, lot number 0703-9812；

Two, method and result

1, thin layer differentiates

(1) thin layer of Radix Salviae Miltiorrhizae differentiates

This product content 2g is taken, water 50ml, ultrasonic extraction 30 minutes is added to let cool, centrifuges, takes supernatant, PH is adjusted with dilute hydrochloric acid Value is 1-2, is extracted 2 times, each 20ml with ethyl acetate, merges ethyl acetate liquid, is evaporated, and residue adds methanol 1ml to make dissolving, is made For test solution；Protocatechualdehyde reference substance separately is taken, adds methanol that solution of every 1ml containing 1mg is made, as a contrast product solution；According to Thin-layered chromatography is tested, and with reference to Chinese Pharmacopoeia one annex VIB of version in 2000, draws 5 μ l of reference substance solution, test solution 10-20 μ l are put on the silica gel g thin-layer plate in same using 0.4% sodium carboxymethylcellulose as binder, respectively with chloroform-acetone- Formic acid is solvent, chloroform：Acetone：The ratio of formic acid is 8：1：1, it is unfolded, takes out, dry, spray is molten with 5% ferric trichloride ethyl alcohol Liquid, it is clear that hot wind is blown to spot development, sets and is inspected under daylight；In test sample chromatography, on position corresponding with reference substance chromatography, The spot of aobvious same color；

(2) thin layer of Radix Astragali differentiates

This product content 3g is taken, methanol 30ml is added, is ultrasonically treated 30 minutes, lets cool, is filtered, filtrate is evaporated, and residue adds water 20ml Make dissolving, be transferred in separatory funnel, adds water saturated n-butanol shaking extraction 2 times, be followed successively by 30ml, 20ml, merge positive fourth Alcohol extract is washed 2 times, each 20ml with 1% sodium hydroxide solution, discards lye, then the water washing 2 being saturated with n-butanol Secondary, each 15ml discards aqueous, and n-butanol liquid is evaporated, and residue adds methanol 1-2ml to make dissolving, as test solution；Separately take Huang Stilbene first glycosides reference substance, adds methanol that solution of every 1ml containing 0.5mg is made, as a contrast product solution；It is tested according to thin-layered chromatography, ginseng According to one VI B of annex of China's coastal port, each 5 μ l of above two solution are drawn, are put respectively in same silica gel g thin-layer plate On, using 10 DEG C of overnight lower layer's solution arranged below of chlorofonn-ethylacetate-methanol-water as solvent, chloroform：Ethyl acetate：First Alcohol：The ratio of water is 15: 40: 22: 10, is unfolded, and takes out, dries, and sprays with 10% ethanol solution of sulfuric acid, spot is heated at 105 DEG C Point colour developing is clear；In test sample chromatography, on position corresponding with reference substance chromatography, the spot of same color is shown；

(3) thin layer of spina date seed differentiates

This product content 4g is taken, water 50ml, ultrasonic extraction 30 minutes is added to let cool, centrifuges, supernatant is taken, with water saturated positive fourth Three times, each 25ml merges n-butanol liquid, is extracted 2 times, each 50ml with ammonia solution, discards ammonia solution, n-butanol liquid for alcohol extracting It is extracted 2 times, each 30ml with distilled water, discards aqueous solution, n-butanol liquid is evaporated, and residue adds methanol 1ml to make dissolving, as examination Product solution；Saponin A reference substance separately is taken, adds methanol that solution of every 1ml containing 1mg is made, as a contrast product solution.According to thin layer Chromatography experiment draws each 5-10 μ l of above two solution with reference to Chinese Pharmacopoeia version one, annex VIB in 2000, put respectively in On same silica gel g thin-layer plate, using 10 DEG C of lower layer's solution arranged below of chloroform-methanol-water as solvent, chloroform:Methanol:Water Ratio is 65：35：10, it is unfolded, takes out, dry, sprays with 1% vanillic aldehyde concentrated sulfuric acid solution, it is clear that hot wind is blown to spot development, sets It is inspected under daylight；In test sample chromatography, on the corresponding position of product chromatography, the spot of same color 1 is shown；

2, assay

(1) chromatographic condition：

Chromatographic column 1 (Hypersil)：5 μ, 4.6mm × 200mm；Chromatographic column 2 (Spherigel)：5 μ, 4.6mm × 200mm；With ten Eight alkyl silane bonded silica gels are filler；- 0.05% phosphoric acid of acetonitrile is mobile phase；Acetonitrile：The ratio of 0.05% phosphoric acid is 20： 80, Detection wavelength 203nm；

(2) preparation of reference substance solution:

Precision weighs ginsenoside Rg1 reference substance 10.78mg, is placed in 10ml volumetric flasks, and methanol is added to make it dissolve in right amount and dilute It releases to scale, product solution as a contrast after shaking up；Precision draws above-mentioned reference substance solution 2.5ml, is placed in 5ml volumetric flasks, adds Methanol dilution is to scale, product dilute solution as a contrast after shaking up, a concentration of 0.539mg/ml of dilute solution；

(3) preparation of test solution：

The a small amount of content of this product is taken, it is finely ground, take 2g, accurately weighed and water-saturated n-butanol 50ml is added, weighed weight, surpasses again Sound extracts 30 minutes, stands overnight, and twice ultrasonic extracts 30 minutes, and weighed weight supplies the weight of less loss with water-saturated n-butanol Amount, filtration discard primary filtrate, collect subsequent filtrate；Precision draws subsequent filtrate 25ml, sets in separatory funnel, with 0.5% hydroxide Sodium water solution washs 2 times, each 10ml, merges butanol solution, and the water 15ml being saturated with n-butanol is washed 1 time, discards washing Liquid, butanol solution water bath method；Residue adds methanol to make to be dissolved in 25ml measuring bottles, adds methanol dilution to scale, shakes up, use is micro- Hole filter membrane filters to obtain the final product, empty a diameter of 0.45 μm of the net of miillpore filter；

(4) selection of Detection wavelength：

Ginsenoside Rg1's reference substance solution 50 μ l, solution concentration 1.078mg/ml are taken, methanol about 5ml is added, with Shimadzu UV-260 It is scanned in 400-200nm wave-length coverages, UV absorption figure is shown in Fig. 1, can be seen that ginsenoside Rg1 from UV absorption Fig. 1 There is absorption peak in 203nm；Therefore select 203nm for ginsenoside Rg1's assay wavelength；

Calibration curve equation is Y=9.163 × 10^-8X+1.074×10^-2R=0.9997；

Sample introduction is ranging from：2.156 μ of μ g~10.078 g；

Calibration curve equation is Y=9.163 × 10^-8X+1.074×10^-2R=0.9997；

Sample introduction is ranging from：2.156 μ of μ g~10.078 g；

(6) precision test：

20,090801 sample of lot number is taken, it is finely ground, 2g is taken, it is accurately weighed, it is tried by test liquid preparation method under " assay " item It tests, precision draws 5 μ l of test liquid, amounts to 5 parts, is injected separately into liquid chromatograph, measures ginsenoside Rg1's peak area and is respectively 20254816,20722368,19968224,20929208,20274486, relative standard deviation 1.90%；

(7) blank test：

Recipe quantity medicinal material is taken, ginseng is removed, negative controls are made by preparation process, then by being tested under " assay " item, make The negative controls solution of standby scarce ginseng crude drug；By negative controls solution, test solution and each 5 μ l of reference substance solution, divide Zhu Ru not be in liquid chromatograph, measurement result shows that negative fluid has no corresponding miscellaneous at ginsenoside Rg1's chromatographic peak retention time Mass peak interferes；

(8) stability test：

20,090801 sample of lot number is taken, it is finely ground, 2g is taken, it is accurately weighed, it is tried by test liquid preparation method under " assay " item It tests, when test liquid is placed 0,4,8 and 12 hour, draws test liquid and each 5 μ l of ginsenoside Rg1's reference substance solution, point Zhu Ru not be in liquid chromatograph, it is respectively 20254816,20557692,20527968,20977204 to measure peak area, opposite to mark Quasi- deviation is 1.45%；From test result it is found that test liquid is placed 12 hours, Determination of Content of Ginsenoside Rg_1 is basicly stable.From above-mentioned As a result it is found that this assay method, test solution precision are good；

(9) reappearance test：

20,090801 sample of lot number is taken, it is finely ground, 2g is taken, it is accurately weighed, it is tried by test liquid preparation method under " assay " item It tests, Determination of Content of Ginsenoside Rg_1 in determination sample, result is：8.86,8.98,9.08,9.02,8.80mg/, average content is 8.94mg/, relative standard deviation 1.29%；From test result it is found that Determination of Content of Ginsenoside Rg_1 measures reappearance test symbol Close regulation；

(10) sample recovery rate is tested：

20,090801 sample of lot number is taken, it is finely ground, 1g is taken, it is accurately weighed, it is tried by test liquid preparation method under " assay " item It tests, amounts to 5 parts, it is accurate respectively that ginsenoside Rg1 reference substance 17mg is added, by being tested under " assay " item, measure mark-on sample Determination of Content of Ginsenoside Rg_1 in product calculates, obtains the rate of recovery of this content assaying method ginsenoside Rg1；Its test result is shown in Table 2；

(11) Determination of Content of Ginsenoside Rg_1 measures in sample:

Each 20 of the sample of different lot numbers is taken, it is finely ground, 2g is taken, it is accurately weighed, by method test under " assay " item, measure people Join saponin(e Rg1 contents, calculates, the result is shown in tables 3；

Determination of Content of Ginsenoside Rg_1 is can be seen that in sample between 5.61mg/ -9.88mg/ from above-mentioned test result, because This will be defined as ginseng in finished product must not be less than 5.0mg in terms of ginsenoside Rg1；

Three, conclusion

1, pharmacodynamic action

A kind of Chinese medicine composition that treating arrhythmia cordis is formed by zhigancao decoction addition or subtraction of changes；Zhigancao decoction is from Zhang Zhongjing 《The Treatise on Fevrile Diseases》, " typhoid fever, intermittent pulse severe palpilation, zhigancao decoction master it " primary efficacy be benefiting qi and nourishing yin, activate yang multiple arteries and veins；A kind for the treatment of The Chinese medicine composition of arrhythmia cordis is by the eight taste Chinese medicine group such as Radix Rehmanniae, ginseng, Radix Astragali, Radix Ophiopogonis, Radix Glycyrrhizae, spina date seed, Radix Salviae Miltiorrhizae, safflower At；Research through modern pharmacology, a kind of Chinese medicine composition that treating arrhythmia cordis have good antiarrhythmic effect；Toast Radix Glycyrrhizae：Effect is invigorating middle warmer and replenishing QI, and relieving spasm to stop pain moistens the lung and relieve the cough, purging intense heat and detonicating, mediation property of medicine；It is sweet in flavor mild-natured, returns spleen, stomach, the heart, Lung channel；Gas and property are slow, and can rise can drop；Modern medicine study, honey-fried licorice root have apparent antiarrhythmic effect, glucocorticoid Sample acts on, anti-oxidant and anti-aging effects；Radix Rehmanniae：Effect is nourishing Yin and clearing heat, and cool blood is enriched blood.It is sweet in flavor, bitter, be slightly cold, the thoughts of returning home, liver, Stomach, the thick drop of matter are let out；Myocardial blood flow amount, reducing heart rate can be increased, and there is certain antihypertensive effect；Radix Astragali：Work( Be it is raw with can invigorating qi for consolidating superficies, inducing diuresis for removing edema；Toast with can tonifying middle-Jiao and Qi, control all laboured breathing syndrome of blood deficiency；Nature and flavor, sweet, tepor enters Lung, the spleen channel；It can significantly improve heart discharge, cardiac index, the often amount of fighting and index of often fighting；Ginseng：It can reinforce vital energy, calm the nerves, Internal lesion caused by overexertion it is deficient, deficiency of food, the card of all qi and blood sperm deficiencies such as burnout；Nature and flavor：It is sweet, slight bitter, temperature；Enter spleen, lung channel；The heart can be enhanced Myotility increases cardiac output, has cardiac effect；Increase coronary blood flow, reducing heart rate, function of resisting myocardial ischemia resists Anoxic and protection cardiac muscle, reduce myocardial oxygen consumption, reduce affinity of the hemoglobin to oxygen, discharge more oxygen to tissue；Wheat Winter：Effect is nourishing Yin and moistening lung, beneficial stomach production of sperm, relieving restlessness that clears away heart-fire；Nature and flavor are sweet, slight bitter, cold nature, return lung, stomach, the heart channel of Hang-Shaoyin；Improve cardiac muscle Convergent force and cardiac pumping function improve tolerance of the mouse ischemic myocardium to bottom oxygen, improve cardiac muscle cell's nutrition, blood flow, Anti-oxidant, antifatigue effect promotes liquid immune；Thus a kind of Chinese medicine composition for treating arrhythmia cordis clinically has very The effect of good treatment arrhythmia cordis；

2, detection method result

This test result establishes a kind of quality determining method of Chinese medicine composition that treating arrhythmia cordis, and ginseng is a kind for the treatment of The main ingredient of the Chinese medicine composition of arrhythmia cordis, ginsenoside Rg1 containing active ingredient, and content is higher, as the master in said preparation Want ingredient, content directly influences therapeutic effect, therefore uses high performance liquid chromatography, the index using it as assay at Point, it can reflect the quality of said preparation, a kind of Chinese medicine composition that treating arrhythmia cordis is using the content of ginsenoside Rg1 as matter The index of amount control is reasonable；Simultaneously using according to thin-layered chromatography, to spina date seed in preparation, Radix Salviae Miltiorrhizae, Radix Astragali has carried out qualitative mirror Not, as a result on position corresponding with reference substance chromatography, the spot of same color, method favorable reproducibility are shown；The quality testing side The foundation of method is for reducing preparation differences between batches, ensureing the quality of the pharmaceutical preparations, ensure that clinical efficacy is of great significance, and method operates Simply, accurate, reproducible, specificity is strong, can be used for controlling the quality of Dingxin capsule.