CN108478607B - Preparation method of sarcandra glabra extract liposome hydrogel - Google Patents
Preparation method of sarcandra glabra extract liposome hydrogel Download PDFInfo
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- CN108478607B CN108478607B CN201810384709.4A CN201810384709A CN108478607B CN 108478607 B CN108478607 B CN 108478607B CN 201810384709 A CN201810384709 A CN 201810384709A CN 108478607 B CN108478607 B CN 108478607B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/28—Steroids, e.g. cholesterol, bile acids or glycyrrhetinic acid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Liposomes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/04—Drugs for disorders of the respiratory system for throat disorders
Abstract
The invention discloses a preparation method of sarcandra glabra extract liposome hydrogel, which comprises the steps of firstly, dissolving lecithin, cholesterol, laurocapram and sarcandra glabra extract in 100-150 mL of chloroform solution, slowly rotating and evaporating under the conditions of 10 ℃ below zero and pressure of-0.065 MPa-0.09 MPa, adding sodium salt solution for mixing after chloroform is completely evaporated, then placing on a shaking bed for shaking for 1-2 h, and placing in a low-temperature ultrasonic instrument for ultrasonic dispersion for 1-3 min until the mixed solution becomes clear and transparent lipid colloid solution; and continuously stirring at the temperature of below 45 ℃, slowly dripping a calcium salt solution into the clear and transparent lipid colloid solution, finishing dripping within 30min, continuously and uniformly stirring, transferring into a packaging container for filling and forming, sealing by using a clean sealing film, and placing in a freezing environment for sealing and storing to obtain the sarcandra glabra extract liposome hydrogel. The paper hydrogel has obvious effect on treating pharyngitis.
Description
Technical Field
The invention belongs to the technical field of health care and medicine, and particularly relates to a preparation method of sarcandra glabra extract liposome hydrogel.
Background
The liposome serving as a preparation type can improve the treatment effect of the medicament, alleviate the adverse reaction of part of the medicament, and improve the formula, and can prepare the liposome with targeting property, thereby further reducing the influence of the medicament on healthy cells. However, since the structure of liposome is easily damaged by the external environment, and the phospholipid in liposome is also easily oxidized to break the liposome, it is difficult to satisfy the stability requirement of pharmaceutical preparation, which also severely restricts the application of liquid liposome. Lyophilized liposomes, however, overcome the disadvantages of liquid liposomes with respect to stability. However, the price of the freeze-drying equipment is high, the energy consumption is high, and the threshold of the liquefaction technology of the freeze-dried liposome is high, so that the application of the freeze-dried liposome is restricted. The hydrogel is in a semi-flowing state, is similar to a jelly state, is combined with the liposome, can provide a water-based environment for the liposome, and can also provide a spatial skeleton structure, so that the structural stability of the liposome is ensured, and the application prospect of the liposome is greatly expanded.
At present, studies using liposome formulations of drugs as transdermal formulations have been reported. Research shows that the complete liposome is very close to the structure of cells, so that the internally wrapped medicine is easy to transfer into epidermal cells, and the structure of the liposome can be preserved for a long time through the wrapping effect of biological macromolecules. However, the liposome structure belongs to a metastable structure and is limited by various factors (some factors are not smooth), so the sarcandra glabra extract liposome is prepared firstly, and then the structure of the liposome is preserved for a long time through the wrapping effect of biological macromolecules; moreover, the selected biological macromolecules have the water retention function at the same time, so that the lipid hydrogel can form osmotic pressure with the interior of the skin, and the release process of the medicine into the body is accelerated
Disclosure of Invention
The invention aims to: aiming at the problems, the invention provides a preparation method of sarcandra glabra extract liposome hydrogel, the paper hydrogel has obvious effect on treating pharyngitis, and can improve the utilization degree of the hydrogel and the user experience; the patient can also take medicine flexibly, and the use risk is reduced. In order to solve the technical problems, the invention adopts the following technical scheme:
in order to achieve the above object, the present invention provides a preparation method of sarcandra glabra extract liposome hydrogel, comprising the following steps:
(1) and preparing sarcandra glabra extract liposome: dissolving 5-15 parts of lecithin, 2.5-5 parts of cholesterol, 1-3 parts of laurocapram and 0.5-2 parts of sarcandra glabra extract in 100-150 mL of chloroform solution, slowly rotating and evaporating under the conditions of the temperature of below 10 ℃ and the pressure of-0.065 MPa to-0.09 MPa, adding a sodium salt solution to mix after the chloroform is completely evaporated, then placing the mixture on a shaking table to shake for 1-2 hours, and placing the mixture in a low-temperature ultrasonic instrument to perform ultrasonic dispersion for 1-3 minutes until the mixed solution becomes a clear and transparent lipid colloid solution;
(2) and preparing the hydrogel: continuously stirring at the temperature of below 45 ℃, slowly dripping a calcium salt solution into the clear and transparent lipid colloid solution, finishing dripping within 30min, continuously and uniformly stirring, transferring into a packaging container for filling and forming, sealing by using a clean sealing film, and placing in a freezing environment for sealing and storing to obtain the sarcandra glabra extract liposome hydrogel.
Preferably, the sodium salt solution added in the step (1) is a sodium alginate solution, and the sodium alginate solution is mixed, wherein the concentration of the added sodium alginate solution is 1%, and the volume of the added sodium alginate solution is 80 mL-100 mL.
Preferably, the sodium salt solution added in step (1) is a sodium phosphate solution, a sodium bicarbonate solution or a sodium dihydrogen phosphate solution, and the mixture is mixed, wherein the pH value of the sodium phosphate solution, the sodium bicarbonate solution or the sodium dihydrogen phosphate solution added in step (1) is 7.3-7.8, and the volume of the solution is 80-120 mL.
Preferably, the calcium salt solution slowly added dropwise in step (2) is a calcium chloride solution, a calcium sulfate solution or a calcium dihydrogen phosphate solution.
In a further preferred embodiment of the above-mentioned aspect, the slowly added calcium chloride solution, calcium sulfate solution or calcium dihydrogen phosphate solution has a concentration of 0.1mol/L and a volume of 20mL to 50 mL.
Preferably, in the step (2), when the stirring is continuously carried out and the stirring is continuously and slowly carried out uniformly at the temperature of 40 ℃, the stirring speed is 20r/min to 50r/min, and the stirring time is 25min to 45 min.
Further preferably, the temperature of the environment in freezing is-2 ℃ to 6 ℃.
In summary, due to the adoption of the technical scheme, the invention has the following technical beneficial effects:
in summary, by adopting the above technical scheme, the beneficial effects of the invention are as follows:
(1) the paper hydrogel has a remarkable effect on treating pharyngitis, the pharyngitis can be treated by selecting a transdermal agent according to analysis of an action part of the pharyngitis, the pharyngitis is taken as a common disease, the traditional Chinese medicine has obvious advantages, however, the content of the traditional Chinese medicine components is influenced by various factors, and the drug effect of the current preparation is not obviously improved, so that a package of solution schemes which can simultaneously solve the problems of medicine stability, bioavailability, onset time and administration frequency is required to be found; the affected part of pharyngitis is close to the epidermis and is a good template disease for the development of transdermal preparations; the traditional Chinese medicine has a large number of formulas and different preparation types to treat pharyngitis, but a transdermal preparation for treating the pharyngitis is not available; pharyngitis belongs to chronic diseases, has long treatment period and is easy to repeatedly attack, the transdermal preparation can reduce the medication times of patients, and the medicine takes effect continuously, so that the patients can obtain better experience.
(2) The liposome hydrogel is mainly used for skin administration, and a transdermal administration system enables people to easily take the medicine and belongs to slow-release administration, so that the risk of blood concentration reduction caused by forgetting to take the medicine and the risk of systemic reaction caused by allergy are avoided. The liver can be protected from the side effect of the medicine, the bioavailability can be improved, and the user experience can be improved; the patient can also flexibly take the medicine, the use risk is reduced, and the medicine taking times and the medicine taking dosage are greatly reduced, so the medicine is very suitable for treating the pharyngitis patient.
(3) The sarcandra glabra extract liposome hydrogel is prepared by determining sarcandra glabra as a template for researching the liposome hydrogel according to a formula principle of treating both symptoms and root causes; selecting proper solvents according to polarity, firstly determining the content extreme values of isofraxidin, chlorogenic acid and rosmarinic acid in the sarcandra glabra, and then utilizing a method of alternately extracting different solvents to enable the content of the three compounds in the extracting solution to approach the extreme values; adsorbing the compounds with low polarity in the extracting solution by using an adsorbent; filtering, and concentrating to obtain a series of Chinese medicinal extracts; measuring the content of effective components in the extract, preparing liposome from the extract, and preparing hydrogel together with biomacromolecule compound; the optimum preparation proportion is determined by using the abdominal skin of a mouse as a template and researching the transdermal efficiency and the dynamic process of the hydrogel, and the research lays a foundation for the research of developing a novel pharyngitis transdermal administration dosage form with reasonable process and controllable quality.
Drawings
FIG. 1 is a comparative analysis chart of the surface morphology of the liposome hydrogel of the sarcandra glabra extract of the present invention;
FIG. 2 is a graph comparing the particle size distribution of the liposome hydrogel of the sarcandra glabra extract of the present invention;
FIG. 3 is a graph comparing the particle size distribution of the liposome hydrogel of sarcandra glabra extract of the present invention;
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below with reference to the accompanying drawings by way of examples of preferred embodiments. It should be noted, however, that the numerous details set forth in the description are merely for the purpose of providing the reader with a thorough understanding of one or more aspects of the invention, even though such aspects of the invention may be practiced without these specific details.
Example 1
According to an aspect of the present invention, the embodiment provides a method for preparing a sarcandra glabra extract liposome hydrogel, comprising the following steps:
(1) and preparing sarcandra glabra extract liposome: dissolving 5g of lecithin, 2.5g of cholesterol, 3g of laurocapram and 0.5g of sarcandra glabra extract in 120mL of chloroform solution, slowly and rotationally evaporating at the temperature of below 10 ℃ and under the pressure of-0.065 MPa, adding a sodium alginate solution with the concentration of 1% and the volume of 80mL after the chloroform is completely evaporated, mixing, placing on a shaking table, shaking for 1h, and placing in a low-temperature ultrasonic instrument for ultrasonic dispersion for 1min until the mixed solution becomes a clear and transparent light yellow lipid colloidal solution;
(2) and preparing the hydrogel: continuously stirring for 25min at the temperature of 35 ℃, stirring at the speed of 20r/min, slowly dropwise adding a calcium chloride solution with the concentration of 0.1mol/L and a calcium chloride solution with the volume of 20mL into the clear and transparent lipid colloid solution, continuously and slowly stirring for 45min at the stirring speed of 20r/min after dropwise adding is finished within 30min, uniformly stirring to obtain a hydrogel mixture, then transferring the hydrogel mixture into a packaging container for containing and forming, sealing by using a clean sealing film, and then sealing and storing in a freezing environment at the temperature of 4-6 ℃ to obtain the sarcandra glabra extract liposome hydrogel.
Example 2
According to an aspect of the present invention, the embodiment provides a method for preparing a sarcandra glabra extract liposome hydrogel, comprising the following steps:
(1) and preparing sarcandra glabra extract liposome: dissolving 15g of lecithin, 5g of cholesterol, 1g of laurocapram and 2g of sarcandra glabra extract in 150mL of chloroform solution, slowly and rotationally evaporating at the temperature of below 5 ℃ and under the pressure of-0.09 MPa, adding a sodium alginate solution with the concentration of 1% and the volume of 100mL after the chloroform is completely evaporated, mixing, then placing on a shaking table, shaking for 2 hours, placing in a low-temperature ultrasonic instrument, and ultrasonically dispersing for 3 minutes until the mixed solution becomes a clear and transparent light yellow lipid colloidal solution;
(2) and preparing the hydrogel: continuously stirring for 45min at the temperature of below 45 ℃, stirring at the speed of 50r/min, slowly dripping a calcium chloride solution with the concentration of 0.1mol/L and a calcium chloride solution with the volume of 30mL into the clear and transparent lipid colloid solution, continuously and slowly stirring for 25min at the stirring speed of 50r/min after dripping is finished within 30min, obtaining a hydrogel mixture after stirring uniformly, then transferring the hydrogel mixture into a packaging container for filling and forming, sealing by using a clean sealing film, and then placing in a refrigerating environment at the temperature of-2 ℃ for sealing and storing to obtain the sarcandra glabra extract liposome hydrogel.
Example 3
According to an aspect of the present invention, the embodiment provides a method for preparing a sarcandra glabra extract liposome hydrogel, comprising the following steps:
(1) and preparing sarcandra glabra extract liposome: dissolving 12g of lecithin, 4g of cholesterol, 2.8g of laurocapram and 1.2g of sarcandra glabra extract in 130mL of chloroform solution, slowly and rotationally evaporating at the temperature of below 10 ℃ and under the pressure of-0.075 MPa, adding a sodium alginate solution with the concentration of 1% and the volume of 100mL after the chloroform is completely evaporated, mixing, then placing on a shaking table, shaking for 1.5h, and placing in a low-temperature ultrasonic instrument for ultrasonic dispersion for 2min until the mixed solution becomes a clear and transparent light yellow lipid colloidal solution;
(2) and preparing the hydrogel: continuously stirring for 30min at the temperature of 40 ℃, stirring at the speed of 35r/min, slowly dripping a calcium chloride solution with the concentration of 0.1mol/L and a calcium chloride solution with the volume of 50mL into the clear and transparent lipid colloid solution, continuously and slowly stirring for 35min at the stirring speed of 30r/min after dripping is finished within 30min, obtaining a hydrogel mixture after stirring uniformly, then transferring the hydrogel mixture into a packaging container for filling and forming, sealing by using a clean sealing film, and then placing in a refrigerating environment at the temperature of 2-4 ℃ for sealing and storing to obtain the sarcandra glabra extract liposome hydrogel.
Example 4
According to one aspect of the present invention, there is provided a method for preparing a sarcandra glabra extract liposome hydrogel, which is similar to example 3, except that sarcandra glabra extract liposome is prepared by adding a sodium phosphate solution, a sodium bicarbonate solution or a sodium dihydrogen phosphate solution with a pH of 7.3 and a volume of 80mL after chloroform is completely evaporated, mixing, then placing on a shaker for shaking for 1.5h, and placing in a low temperature ultrasonic apparatus for ultrasonic dispersion until the mixed solution becomes a clear and transparent pale yellow lipid colloidal solution; in the preparation of hydrogel, calcium sulfate solution or calcium dihydrogen phosphate solution with concentration of 0.1mol/L and volume of 35mL is slowly dropped into the prepared liposome hydrogel.
Example 5
This example provides a method for preparing a sarcandra glabra extract liposome hydrogel according to an aspect of the present invention, which is similar to example 4, except that sarcandra glabra extract liposome is prepared by adding a sodium phosphate solution, a sodium bicarbonate solution or a sodium dihydrogen phosphate solution having a pH of 7.8 and a volume of 120mL after chloroform is completely evaporated, and mixing; in the preparation of hydrogel, calcium sulfate solution or calcium dihydrogen phosphate solution with concentration of 0.1mol/L and volume of 40mL is slowly dropped into the prepared liposome hydrogel.
Example 6
This example provides a method for preparing a sarcandra glabra extract liposome hydrogel according to an aspect of the present invention, which is similar to example 4, except that sarcandra glabra extract liposome is prepared by adding a sodium phosphate solution, a sodium bicarbonate solution or a sodium dihydrogen phosphate solution having a pH of 7.4 and a volume of 90mL after chloroform is completely evaporated, and mixing; in the preparation of hydrogel, calcium sulfate solution or calcium dihydrogen phosphate solution with concentration of 0.1mol/L and volume of 45mL is slowly dropped into the prepared liposome hydrogel.
In summary, in order to further understand the technical features of the present invention, the present invention is further described below with reference to the accompanying drawings, example 3 is selected as a representative test, and the prepared sarcandra glabra extract liposome hydrogel is tested under a scanning electron microscope; firstly, blank reference substance 1 liposome hydrogel and blank reference substance 2 liposome hydrogel are prepared, and then surface morphology analysis and comparison are carried out on the blank reference substance 1 liposome hydrogel and the blank reference substance 2 liposome hydrogel.
First, surface morphology analysis and comparison
1. Preparation of blank control 1: preparation of blank liposome 1 (in comparison to sodium alginate added in example 3):
dissolving 12g of lecithin, 4g of cholesterol and 2.8g of laurocapram in 130ml of chloroform solution, slowly rotating and evaporating at 10 ℃, adding 100ml of 1% sodium alginate solution for mixing after the chloroform solution (solvent) is completely evaporated, shaking on a shaking table for 1.5 hours, and placing in a low-temperature ultrasonic instrument for ultrasonic dispersion for 2min until the mixed solution becomes a clear and transparent colorless transparent colloidal solution.
2. Preparation of blank control 2: preparing blank liposome 2 (comparing with added phosphate), dissolving lecithin 12g, cholesterol 4g, and laurocapram 2.8g in chloroform solution 130ml, slowly rotary evaporating at 10 deg.C until the solvent is completely evaporated, adding phosphate buffer solution 90ml with pH7.4, shaking on shaking table for 1.5 hr, and ultrasonic dispersing in low temperature ultrasonic instrument for 2 min. Until the mixture became clear and transparent colorless and transparent colloidal solution.
As can be seen from the electron microscope image, all three liposomes are approximately spherical, the blank liposome 1 has uneven size distribution, as shown in the image a in FIG. 1, and is easy to aggregate into lumps; the comprehensive radius distribution of the blank liposome 2 is slightly larger than that of the blank liposome, as shown in a graph b in a graph 1, the blank liposome is related to embedded drugs and is similar to the blank liposome and is easy to aggregate into blocks; the radius of the sarcandra glabra extract liposome hydrogel is the largest, as shown in a graph c in a graph 1, the distribution is also the most uniform, and a photo shows that no agglomeration tendency exists among liposomes, which is related to isolation among the liposomes caused by the wrapping of sodium alginate, so that the sarcandra glabra extract liposome aqueous solution can form osmotic pressure, the sarcandra glabra encapsulation rate can be greatly improved, the particle size distribution of the sarcandra glabra extract liposome is more even, as shown in a graph 2, the main particle size distribution of a blank liposome 1 is about 100nm, the distribution area is wider, and the distribution area is also consistent with an electron microscope photo in the graph 1; the particle size distribution of the blank liposome 2 is slightly larger than that of the blank liposome (140-150nm), and is related to the embedded drug; the peak intensity and distribution interval show that the sarcandra glabra extract liposome hydrogel (sodium alginate solution) is most uniformly distributed, and the peak value is about 200 nm.
Preparation of lipid hydrogel transdermal experiment of sarcandra glabra extract
1. Preparation of mouse abdominal skin: healthy mice were sacrificed and then the abdominal skin was stripped and the skin was carefully removed of hair and subcutaneous fat, taking care to maintain the integrity of the skin.
2. Hourly cumulative transdermal test
The treated mouse skin was observed under a microscope to confirm no damage. The skin of the mice was fixed to a Franz vertical diffuser (diffusion radius 1 cm); the dermis layer of the mouse skin was determined to be towards the receiving surface and the stratum corneum layer towards the administration surface. After fixation, excess skin was removed and the receiving surface was filled with phosphate buffer (pH7.4) in the following system:
1) adopting a blank reference substance 1 system as a control experiment to determine whether the interference exists in the mouse skin extract in the analysis method;
2) adopting a blank reference substance 2 system as a reference experiment to determine whether the interference exists in the mouse skin extract in the analysis method;
3) the sarcandra glabra extract lipid hydrogel system is used as a control experiment to determine whether the skin extract of the mouse has interference in the analysis method;
the sampling time is respectively as follows: the cumulative amount of the transdermal drug was calculated by determining the drug system concentration using a high performance liquid chromatograph and a standard curve for 2 hours, 4 hours, 8 hours, 12 hours, 18 hours, and 24 hours. Each group is operated in parallel for 3 times. Until the transdermal reaches the balance, carefully observing the skin before and after the reaction by using a microscope to determine whether the abdominal skin of the mouse is complete or not so as to ensure that the experimental result is real and reliable; the cumulative transdermal capacity of the lipid hydrogel can be calculated as follows:
g is the accumulated transdermal mass; v is the receiving pool volume; v0Is the sampling volume; cnThe drug concentration measured for the last sample point; ciThe drug concentration measured for the ith sample point; a is the diffusion area of the Franz vertical diffuser;
in-vitro transdermal experimental simulation is carried out by utilizing mouse abdominal skin, and the curve of the relation between the accumulated transdermal amounts of the blank reference substance 1, the blank reference substance 2 and the sarcandra glabra extract lipid hydrogel system and time is determined to be shown in figure 3, as can be seen from figure 3, the transdermal capacity of the sarcandra glabra extract lipid hydrogel system is obviously improved, and the transdermal amount of the sarcandra glabra extract lipid hydrogel system is improved by 50 percent compared with that of the blank reference substance 1 and the blank reference substance 2.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.
Claims (6)
1. A preparation method of sarcandra glabra extract liposome hydrogel is characterized by comprising the following steps: the method comprises the following steps:
(1) and preparing sarcandra glabra extract liposome: dissolving 5-15 parts of lecithin, 2.5-5 parts of cholesterol, 1-3 parts of laurocapram and 0.5-2 parts of sarcandra glabra extract in 100-150 mL of chloroform solution, slowly rotating and evaporating under the conditions of the temperature of below 10 ℃ and the pressure of-0.065 MPa to-0.09 MPa, adding a sodium alginate solution, a sodium phosphate solution or a sodium dihydrogen phosphate solution to mix after the chloroform is completely evaporated, then placing on a shaking table, shaking for 1-2 hours, and placing in a low-temperature ultrasonic instrument for ultrasonic dispersion for 1-3 minutes until the mixed solution becomes a clear and transparent lipid colloidal solution;
(2) and preparing the hydrogel: continuously stirring at the temperature of below 45 ℃, slowly dripping a calcium salt solution into the clarified and transparent lipid colloid solution, finishing dripping within 30min, continuously and uniformly stirring, transferring into a packaging container for filling and forming, sealing by using a clean sealing film, and then placing into a freezer at the temperature of-2-6 ℃ for environmental sealing and storing to obtain the sarcandra glabra extract liposome hydrogel.
2. The method for preparing the sarcandra glabra extract liposome hydrogel according to claim 1, wherein the method comprises the following steps: the concentration of the sodium alginate solution added in the step (1) is 1%, and the volume is 80 mL-100 mL.
3. The method for preparing the sarcandra glabra extract liposome hydrogel according to claim 1, wherein the method comprises the following steps: the pH value of the sodium phosphate solution or the sodium dihydrogen phosphate solution added in the step (1) is 7.3-7.8, and the volume is 80-120 mL.
4. The method for preparing the sarcandra glabra extract liposome hydrogel according to claim 1, wherein the method comprises the following steps: the calcium salt solution slowly dripped in the step (2) is a calcium chloride solution, a calcium sulfate solution or a calcium dihydrogen phosphate solution.
5. The method for preparing the sarcandra glabra extract liposome hydrogel according to claim 4, wherein the method comprises the following steps: the slowly-dropwise-added calcium chloride solution, calcium sulfate solution or calcium dihydrogen phosphate solution is a calcium chloride solution with the concentration of 0.1mol/L, and the volume is 20 mL-50 mL.
6. The method for preparing the sarcandra glabra extract liposome hydrogel according to claim 1, wherein the method comprises the following steps: and (3) continuously stirring and continuously slowly stirring uniformly at the temperature of 40 ℃ in the step (2), wherein the stirring speed is 20-50 r/min, and the stirring time is 25-45 min.
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