CN108409417A - A kind of microbial manure prepared by frankia - Google Patents
A kind of microbial manure prepared by frankia Download PDFInfo
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- CN108409417A CN108409417A CN201810339120.2A CN201810339120A CN108409417A CN 108409417 A CN108409417 A CN 108409417A CN 201810339120 A CN201810339120 A CN 201810339120A CN 108409417 A CN108409417 A CN 108409417A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/04—Double-superphosphate; Triple-superphosphate; Other fertilisers based essentially on monocalcium phosphate
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Fertilizers (AREA)
Abstract
The present invention discloses a kind of microbial manure prepared by frankia, includes the component of following weight ratio：10 20 parts of 40 60 parts of dregs of beans, 20 40 parts of wheat bran, 20 50 parts of nitrogen, phosphorus and potassium fertilizer, 14 parts of magnesium sulfate, 1 10 parts of liquid microelement, 15 parts of frankia bacterium powder, 13 parts of microbial bacterial agent and charcoal.The microbial manure contains the active principles such as frankia bacterium powder, liquid microelement, microbial bacterial agent, by scientific matching, each component interacts, greatly improve its nitrogen fixation efficiency, herbaceous plant nodulation and nitrogen fixation can successfully be infected, and with the nutrient for improving crop nutritional condition, fixing some invalid states in nitrogen and activating soil, create the effect of good soil microenvironment.
Description
Technical field
The present invention relates to microbial manure field, especially a kind of microbial manure of frankia preparation.
Background technology
Microbial manure is to cause crop to obtain a kind of product of specific fertilizer effect with the vital movement of microorganism, is agriculture
One kind of fertilizer is used in industry production.Microbial manure contains a large amount of beneficial microbes, can improve crop nutritional condition, fixation
The nutrient of the invalid state of some in nitrogen and activating soil creates good soil microenvironment to promote the life of crop
It is long.Proof is tested in the world for many years, and symbiotic nitrogen fixation efficiency can be improved with legumes such as legume inoculation soybean, peanuts, have really
Effect of increasing production is rationally dressed seed or is applied microbial manure using other bacterial manure, also has effect of increasing production to non-legume crops, and
There is the effect that chemical fertilizer is not achieved.
Frank (Frankia) bacterium is a unique category that can fix nitrogen in air in Actinomycetal.Its fixed nitrogen
Amount more than 3-5 times or more of Rhizobium leguminosarum, accounts for 60% or more of symbiotic nitrogen fixation total amount, accounts for 80% of sources of nitrogen on the earth again
More than.As alder root Frank's bacterium amount of nitrogen fixation is 85-300 kilograms of nitrogen/hectare/year (kg N2/ha/y), autumn alder amount of nitrogen fixation
For 40-157, red branch alder is 140-209, and sea-buckthorn is 47~179, horse-tail 229, Coriaria sinica 150.1kg in fixed air
N2 elements are equivalent to the urea for applying 2.5kg, their 1 year suitable mus of amount of nitrogen fixation of these plants apply the urea of 24-50kg, and soybean
Only quite mu applies 14kg urea.With the growth of the actnorhizal plant age of tree, there are newly-increased 50% or more newborn root nodule, institute every year
Incrementally increased with being added to for the age of tree with fixed nitrogen total amount.As it can be seen that Frank's bacterium nodulation and nitrogen fixation, on the earth using compound state
Nitrogen cycle and the ecological balance play particularly important effect in the source of nitrogen and terrestrial ecosystems, while also having afforested the earth,
Soil fertility is improved, human survival condition and quality are created and improve, plays huge ecological benefits.
Frank bacterium has the function of good nodulation and nitrogen fixation, if applied in microbial manure, it will greatly improve
Its nitrogen fixation efficiency.But so far, forefathers scholars can only infect nodulation and nitrogen fixation using frankia on xylophyta,
But herbaceous plant nodulation and nitrogen fixation cannot be infected, keeps the application of frankia significantly limited.How to configure using Frank bacterium
Microbial manure, make its effect reach more preferably be existing research an emphasis.
Invention content
In order to overcome the disadvantages mentioned above of the prior art, the object of the present invention is to provide micro- lifes prepared by a kind of frankia
Object fertilizer, the microbial manure can successfully infect herbaceous plant nodulation and nitrogen fixation, and having improves crop nutritional condition, fixed nitrogen
With the nutrient of the invalid state of some in activating soil, the effect of good soil microenvironment is created.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of microbial manure prepared by frankia, which includes the component of following weight ratio：Dregs of beans
40-60 parts, 20-40 parts of wheat bran, 20-50 parts of nitrogen, phosphorus and potassium fertilizer, 1-4 parts of magnesium sulfate, 1-10 parts of liquid microelement, frankia bacterium
10-20 parts of 1-5 parts of powder, 1-3 parts of microbial bacterial agent and charcoal.
As a further improvement on the present invention：The microbial manure includes the component of following weight ratio：Dregs of beans 45-55
Part, 25-35 parts of wheat bran, 30-40 parts of nitrogen, phosphorus and potassium fertilizer, 2-3 parts of magnesium sulfate, 4-6 parts of liquid microelement, frankia bacterium powder 2-4
12-18 parts of part, 1.5-2.5 parts of microbial bacterial agent and charcoal.
As a further improvement on the present invention：The microbial manure includes the component of following weight ratio：50 parts of dregs of beans,
30 parts of wheat bran, 35 parts of nitrogen, phosphorus and potassium fertilizer, 2.5 parts of magnesium sulfate, 5 parts of liquid microelement, 3 parts of frankia bacterium powder, microbial bacterial agent 2
15 parts of part and charcoal.
As a further improvement on the present invention：The microbial bacterial agent includes phosphate-solubilizing bacteria and potassium bacterium.
As a further improvement on the present invention：The liquid microelement includes boric acid, two water manganese chlorides, seven water sulfuric acid
Zinc, cupric sulfate pentahydrate, two water sodium manganates and calcium chloride hexahydrate.
As a further improvement on the present invention：The nitrogen, phosphorus and potassium fertilizer includes urea, one-lime phosphate and potassium sulfate.
As a further improvement on the present invention：The frankia refers to the 30- obtained using the method for Mutagenesis
Frank that 60min breeds a generation, grows mycelia for 5-7 days under conditions of suitable yeasting and raw material, differentiates spore
Salmonella.
As a further improvement on the present invention：The charcoal is to be carbonized to be formed under the conditions of 500-700 DEG C by stalk
Biological activated carbon.
As a further improvement on the present invention：The preparation method of the microbial manure includes：Each component is distinguished into powder
Broken sieving, it is then uniform in stirrer for mixing, it is granulated, freeze-drying, screening, packs, you can obtain the microbial manure.
Compared with prior art, the beneficial effects of the invention are as follows：
The microbial manure contains the active principles such as frankia bacterium powder, liquid microelement, microbial bacterial agent, by section
Proportioning is learned, each component interaction greatly improves its nitrogen fixation efficiency, can successfully infect herbaceous plant nodulation and nitrogen fixation, and
With the nutrient for improving crop nutritional condition, fixing some invalid states in nitrogen and activating soil, good soil is created
The effect of micro-ecological environment.
The microbial manure has the function of certain fertilizer and certain production-increasing function, be effectively improved conventional fertilizers utilization rate it is low,
Utilization rate of fertilizer can be improved in a series of problems caused by excessive application etc., correct application, reaches the mesh that synergy is increased production, decrement drop is dirty
, it is efficient development, green, the inevitable requirement of sustainable agriculture.
Description of the drawings
Fig. 1 is the fungistatic effect figure of frankia.
Fig. 2 is the gaining effect figure of four kinds of vegetables.
Fig. 3, which is that four kinds of frankia pair is herbal, infects design sketch.
Fig. 4 is content of organic matter variation diagram in soil.
Fig. 5 is hydrolyzable nitrogen changes of contents figure in soil.
Fig. 6 is available phosphorus content variation diagram in soil.
Fig. 7 is quick-acting potassium content variation diagram in soil.
Specific implementation mode
In conjunction with description of the drawings, the present invention is further described with embodiment：
Embodiment one：
A kind of microbial manure prepared by frankia, formula：Include the component of following weight ratio：50 parts of dregs of beans, wheat
30 parts of bran, 35 parts of nitrogen, phosphorus and potassium fertilizer, 2.5 parts of magnesium sulfate, 5 parts of liquid microelement, 3 parts of frankia bacterium powder, 2 parts of microbial bacterial agent
With 15 parts of charcoal.
Preparation method：Each component is pulverized and sieved respectively, it is then uniform in stirrer for mixing, be granulated, freeze-drying, sieve,
Packaging, you can obtain the microbial manure.
Embodiment two：
A kind of microbial manure prepared by frankia, formula：Include the component of following weight ratio：40 parts of dregs of beans, wheat
22 parts of bran, 22 parts of nitrogen, phosphorus and potassium fertilizer, 1.5 parts of magnesium sulfate, 2.3 parts of liquid microelement, 1.9 parts of frankia bacterium powder, microbial bacteria
11 parts of 1.2 parts of agent and charcoal.
Preparation method：Each component is pulverized and sieved respectively, it is then uniform in stirrer for mixing, be granulated, freeze-drying, sieve,
Packaging, you can obtain the microbial manure.
Embodiment three：
A kind of microbial manure prepared by frankia, formula：Include the component of following weight ratio：58 parts of dregs of beans, wheat
38 parts of bran, 45 parts of nitrogen, phosphorus and potassium fertilizer, 3.6 parts of magnesium sulfate, 9.2 parts of liquid microelement, 4.3 parts of frankia bacterium powder, microbial bacteria
18.8 parts of 2.6 parts of agent and charcoal.
Preparation method：Each component is pulverized and sieved respectively, it is then uniform in stirrer for mixing, be granulated, freeze-drying, sieve,
Packaging, you can obtain the microbial manure.
Example IV：
A kind of microbial manure prepared by frankia, formula：Include the component of following weight ratio：45 parts of dregs of beans, wheat
35 parts of bran, 30.5 parts of nitrogen, phosphorus and potassium fertilizer, 2.2 parts of magnesium sulfate, 4.2 parts of liquid microelement, 2.1 parts of frankia bacterium powder, microorganism
12.3 parts of 2.5 parts of microbial inoculum and charcoal.
Preparation method：Each component is pulverized and sieved respectively, it is then uniform in stirrer for mixing, it is granulated, freeze-drying, sieves
Divide, packaging, you can obtain the microbial manure.
Embodiment five：
A kind of microbial manure prepared by frankia, formula：Include the component of following weight ratio：55 parts of dregs of beans, wheat
25 parts of bran, 39.5 parts of nitrogen, phosphorus and potassium fertilizer, 2.8 parts of magnesium sulfate, 5.8 parts of liquid microelement, 3.6 parts of frankia bacterium powder, microorganism
17.6 parts of 1.5 parts of microbial inoculum and charcoal.
Preparation method：Each component is pulverized and sieved respectively, it is then uniform in stirrer for mixing, be granulated, freeze-drying, sieve,
Packaging, you can obtain the microbial manure.
Experimental example one：The toxicological experiment of frankia
Using frankia bacterium solution to intragastric administration on mice, the toxicological experiment of frankia is carried out.
1 frankia bacterium solution of table is to mouse maximum tolerance determination
From table 1 the results showed that using frankia bacterium solution to intragastric administration on mice, when concentration reaches 1.5g/ml, have after 3 days
One death；When concentration is increased to 2.0g/ml, there are 3 death in the 2nd day to 4 days.Mouse shows in addition to activity is reduced,
Without other any abnormal responses.Through Anatomical Observation, the cause of death is mouse stomach mucous modification, this may be that excessively high dose is hindered
Caused by having done harm to gastric mucosa.Therefore, the LD50 of Frank bacterium is calculated by 1.5g/ml：Mouse maximal tolerance dose multiple=(1.5/
21.68) × (70000g/30g)=154 times.It fully proves that Frank bacterium extracting solution is not substantially toxic, belongs to nontoxic grade.
Experimental example two：The bacteriostatic experiment of frankia
It is caused a disease to staphylococcus aureus, Cabbage Leaf Spot, Escherichia coli, four kinds of saccharomycete using frankia bacterium solution
Bacterium carries out bacteriostatic experiment.
From Fig. 1 the results showed that the lethality of four kinds of pathogenic bacteria of frankia bacterium solution pair with the increase of bacterial concentration and
Become strong, it is especially stronger to the lethality of Escherichia coli and Cabbage Leaf Spot, it can effectively inhibit the growth of some pathogenic bacteria.
Experimental example three:Frankia infects experiment
Be added the frankia bacterium solution of Artificial Growth element to corn, okra, wheat, four kinds of herbaceous plant of sorghum into
Row infects experiment.
From Fig. 3 the results showed that the frankia bacterium solution success after Artificial Growth element is added is tied on four kinds of herbaceous plant
Tumor fixed nitrogen, wherein dross it is most be okra and corn, dross rate is up to 70.5%~100%.After one month, also with acetylene
Former method determines nitrogenase activity in okra root nodule, up to 77.6nmol/ plants/day, is equivalent to triennial Hippophae nodule fixed nitrogen
The 71.2% of enzyme activity.With the mycelia with the presence of Frank bacterium in transmission electron microscope observing to corn root nodule, scanning electron microscopic observation is used
There are more spores in corn nodule cell.
Experimental example four:The field experiment of four kinds of vegetables of bacterial manure pair
Using the microbial manure of embodiment one as experimental group, using certain microbial manure for generally selling on the market as
Control group is administered to four kinds of leek, spinach, cucumber, tomato vegetables, carries out 14 days by a definite date field experiments.
From Fig. 2 the results showed that the experimental group vegetables weight using microbial manure is apparently higher than control group, absolutely prove
The microbial manure can effectively facilitate the growth of vegetables.
Experimental example five:Improved experimental of the frankia bacterium powder to soil
Using frankia bacterium powder as experimental group, as a control group with peat soil, it is administered to soil, carried out 180 days by a definite date
Improved experimental.
From Fig. 4, Fig. 5, Fig. 6 and Fig. 7 the results showed that compared with control group is using peat soil, using frankia bacterium powder
Soil in the content of organic matter, hydrolysis nitrogen content, available phosphorus content and quick-acting potassium content all significantly promoted, fully prove Forlan
Kirschner bacterium bacterium powder can be good at conditioned soil property improvement, substantially increase the vigor of soil.
Claims (9)
1. microbial manure prepared by a kind of frankia, it is characterised in that：The microbial manure includes following weight
The component of ratio：40-60 parts of dregs of beans, 20-40 parts of wheat bran, 20-50 parts of nitrogen, phosphorus and potassium fertilizer, 1-4 parts of magnesium sulfate, liquid microelement 1-10
Part, 1-5 parts of frankia bacterium powder, 1-3 parts of microbial bacterial agent and 10-20 parts of charcoal.
2. microbial manure prepared by a kind of frankia according to claim 1, it is characterised in that：Micro- life
Object fertilizer includes the component of following weight ratio：45-55 parts of dregs of beans, 25-35 parts of wheat bran, 30-40 parts of nitrogen, phosphorus and potassium fertilizer, magnesium sulfate 2-3
Part, 4-6 parts of liquid microelement, 2-4 parts of frankia bacterium powder, 1.5-2.5 parts of microbial bacterial agent and 12-18 parts of charcoal.
3. microbial manure prepared by a kind of frankia according to claim 2, it is characterised in that：Micro- life
Object fertilizer includes the component of following weight ratio：50 parts of dregs of beans, 30 parts of wheat bran, 35 parts of nitrogen, phosphorus and potassium fertilizer, 2.5 parts of magnesium sulfate, micro member
15 parts of 5 parts of plain liquid, 3 parts of frankia bacterium powder, 2 parts of microbial bacterial agent and charcoal.
4. the microbial manure prepared according to a kind of any frankias of claim 1-3, it is characterised in that：It is described
Microbial bacterial agent include phosphate-solubilizing bacteria and potassium bacterium.
5. the microbial manure prepared according to a kind of any frankias of claim 1-3, it is characterised in that：It is described
Liquid microelement include boric acid, two water manganese chlorides, white vitriol, cupric sulfate pentahydrate, two water sodium manganates and calcium chloride hexahydrate.
6. the microbial manure prepared according to a kind of any frankias of claim 1-3, it is characterised in that：It is described
Nitrogen, phosphorus and potassium fertilizer include urea, one-lime phosphate and potassium sulfate.
7. the microbial manure prepared according to a kind of any frankias of claim 1-3, it is characterised in that：It is described
Frankia refer to the 30-60min breedings generation obtained using the method for Mutagenesis, in suitable yeasting and raw material
Under conditions of the frankia that grows mycelia for 5-7 days, differentiate spore.
8. the microbial manure prepared according to a kind of any frankias of claim 1-3, it is characterised in that：It is described
Charcoal be the biological activated carbon of formation of being carbonized under the conditions of 500-700 DEG C by stalk.
9. the microbial manure prepared according to a kind of any frankias of claim 1-3, it is characterised in that：It is described
The preparation method of microbial manure include：Each component is pulverized and sieved respectively, it is then uniform in stirrer for mixing, be granulated,
Freeze-drying, screening, packaging, you can obtain the microbial manure.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111676178A (en) * | 2020-07-26 | 2020-09-18 | 吉林省弘雨环境科技有限公司 | Frankia ginseng biocontrol microbial inoculum and soil improvement method |
| US11124461B2 (en) | 2019-07-04 | 2021-09-21 | Incitec Pivot Limited | Fertilizer |
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Application publication date: 20180817 |