CN108324800B

CN108324800B - Chinese herbal medicine compound extract, preparation method and application thereof, and medicine or cosmetic

Info

Publication number: CN108324800B
Application number: CN201810464320.0A
Authority: CN
Inventors: 韩晓辉; 赵爽; 赵园
Original assignee: Guangzhou Kefan Biological Technology Co ltd
Current assignee: Guangzhou Kefan Biological Technology Co ltd
Priority date: 2018-05-15
Filing date: 2018-05-15
Publication date: 2021-03-23
Anticipated expiration: 2038-05-15
Also published as: CN108324800A

Abstract

The invention provides a Chinese herbal medicine compound extract, a preparation method and application thereof, and a medicine or a cosmetic, and relates to the technical field of Chinese herbal medicine compound extracts. A Chinese herbal medicine compound extract comprises the following components: ligusticum wallichii root extract, angelica sinensis root extract, Shengma yuen extract, divaricate saposhnikovia root extract, medlar root extract, water and optional auxiliary materials. The traditional Chinese medicine composition disclosed by the invention overcomes the defect that the skin allergy problem is easy to relapse in the traditional western medicine solution, and is simple in formula, low in toxic and side effects and good in anti-inflammatory and itching relieving effects. In addition, the invention provides a preparation method of the Chinese herbal medicine compound extract, and the preparation method has the advantages of simple process, high extraction rate, low cost and short time. The Chinese herbal medicine compound extract can be applied to medicines or cosmetics, and medicines or cosmetics containing the Chinese herbal medicine compound extract are obtained.

Description

Chinese herbal medicine compound extract, preparation method and application thereof, and medicine or cosmetic

Technical Field

The invention relates to the technical field of Chinese herbal medicine extracts, and particularly relates to a Chinese herbal medicine compound extract, a preparation method and application thereof, and a medicine or a cosmetic.

Background

With the development of social economy, people have more and more resistance decline due to factors such as dietary habits, living pressure, natural environment and the like, people with various skin sensitivities tend to rise, the skin problems are always stubborn, inflammatory reactions caused by the skin sensitivities are not easy to remove, and the skin sensitivities are easy to relapse after treatment.

At present, the medicines for treating skin problems mainly comprise western medicines which are commonly used as follows: antihistamines: loratadine, cetirizine and urticaria pills. Hydrocortisone cream and triamcinolone acetonide acetate urea ointment, and even though many western medicines are treated by various administration modes with different degrees, skin problems can be relieved or repaired to a certain degree, but the skin problems are difficult to treat fundamentally. But the most common problems are that the traditional Chinese medicine composition is easy to repeatedly attack, difficult to fundamentally improve and often has toxic and side effects.

The existing Chinese herbal medicine or cosmetic aiming at the problems of sensitive, itchy or red skin and the like has complex formula and undesirable effect, still cannot achieve the effect of deep treatment to a certain extent, and is easy to relapse due to incomplete treatment. Therefore, the development of the Chinese herbal medicine compound extract which has the advantages of simple formula, natural components, good effect, difficult relapse, low toxic and side effects, fundamentally improving the body resistance and achieving better anti-inflammatory and itching-relieving effects is an important work in the technical field of improving skin health at present.

In view of the above, the present invention is particularly proposed.

Disclosure of Invention

The invention aims to provide a Chinese herbal medicine compound extract, which comprises a ligusticum wallichii root extract, a angelica sinensis root extract, a cimicifuga root extract, a radix sileris extract, a lycium barbarum root extract, water and optional auxiliary materials; the defect that the skin allergy problem is easy to relapse in the traditional western medicine solution is overcome, and the Chinese medicinal composition is simple in formula, low in toxic and side effects and good in anti-inflammatory and itching relieving effects.

The second purpose of the invention is to provide the preparation method of the Chinese herbal medicine compound extract, which has the advantages of simple process, high extraction rate, low cost and short time.

The invention also aims to provide the application of the Chinese herbal medicine compound extract prepared by the preparation method of the Chinese herbal medicine compound extract or the Chinese herbal medicine compound extract in preparing anti-inflammatory and antipruritic medicines or cosmetics.

The fourth purpose of the invention is to provide a medicine or a cosmetic containing the Chinese herbal medicine compound extract or the Chinese herbal medicine compound extract prepared by the preparation method of the Chinese herbal medicine compound extract.

In order to achieve the above purpose of the present invention, the following technical solutions are adopted:

in a first aspect, the invention provides a Chinese herbal medicine compound extract, which comprises the following components: ligusticum wallichii root extract, angelica sinensis root extract, Shengma yuen extract, divaricate saposhnikovia root extract, medlar root extract, water and optional auxiliary materials.

Preferably, on the basis of the scheme of the invention, the Chinese herbal medicine compound extract comprises the following components in percentage by mass: 1-5% of ligusticum wallichii root extract, 1-5% of angelica sinensis root extract, 1-5% of radix cimicifugae extract, 1-5% of radix sileris extract, 1-5% of lycium barbarum root extract, 0.1-1% of auxiliary material and the balance of water.

Preferably, the Chinese herbal medicine compound extract comprises the following components in percentage by mass: 1-5% of ligusticum wallichii root extract, 2-5% of angelica sinensis root extract, 1-5% of radix cimicifugae extract, 2-5% of radix sileris extract, 1-5% of lycium barbarum root extract, 0.1-1% of auxiliary material and the balance of water;

preferably, the Chinese herbal medicine compound extract comprises the following components in percentage by mass: 2-5% of ligusticum wallichii root extract, 3-5% of angelica sinensis root extract, 2-5% of radix cimicifugae extract, 3-5% of radix sileris extract, 2-5% of wolfberry root extract, 0.1-1% of auxiliary material and the balance of water.

Preferably, on the basis of the scheme of the invention, the auxiliary materials comprise one or more of a sweetening agent, an aromatic agent, a mucilage, an antioxidant, a surfactant, a humectant or a preservative.

Preferably, on the basis of the scheme of the invention, the sweetener comprises one or more of sucrose, fructose or glucose;

preferably, the flavoring agent comprises one or more of lemon, peppermint oil or essence;

preferably, the mucilage comprises one or more of sodium alginate, acacia, gelatin, methylcellulose or sodium carboxymethylcellulose;

preferably, the antioxidant comprises one or more of vitamin A, vitamin C, vitamin D, vitamin E, alpha-lipoic acid or coenzyme Q10;

preferably, the surfactant comprises one or more of phospholipid, choline, protein or sodium stearate;

preferably, the humectant comprises one or more of glycerol, collagen, hyaluronic acid or amino acid;

preferably, the preservative comprises sodium benzoate and/or potassium sorbate.

Preferably, on the basis of the scheme of the invention, the Chinese herbal medicine compound extract comprises the following components in percentage by mass: 1-5% of ligusticum wallichii root extract, 1-5% of angelica sinensis root extract, 1-5% of dahurian angelica root extract, 1-5% of divaricate saposhnikovia root extract, 1-5% of lycium barbarum root extract, 0.3-0.6% of sodium benzoate, 0.1-0.3% of potassium sorbate and the balance of water.

In a second aspect, the invention provides a preparation method of the Chinese herbal medicine compound extract, which comprises the following steps:

mixing rhizoma Ligustici Chuanxiong root extract, radix Angelicae sinensis extract, radix Helicteris extract, radix Saposhnikoviae extract and Lycii radix extract, water and optional adjuvants uniformly to obtain Chinese herbal compound extract.

Preferably, on the basis of the scheme of the invention, the ligusticum wallichii root extract is obtained by putting the crushed ligusticum wallichii root into ethanol with the volume fraction of 65-85% for extraction;

preferably, the angelica root extract is obtained by extracting crushed angelica root in ethanol with the volume fraction of 70-85%;

preferably, the Shengma Magen extract is obtained by extracting a crushed Shengma Magen material in 65-80% ethanol by volume fraction;

preferably, the radix sileris extract is obtained by putting the radix sileris crushed material into ethanol with the volume fraction of 65-85 percent for extraction;

preferably, the wolfberry root extract is obtained by extracting crushed wolfberry root in 65-85 vol% ethanol;

preferably, on the basis of the scheme of the invention, the extraction method of the ligusticum wallichii root extract comprises the following steps: soaking the crushed rhizoma ligustici wallichii root in 65-85% ethanol for 30-60 min, extracting for 20-60 min in a water bath at the temperature of 75-85 ℃, repeatedly extracting for 2-3 times, and removing the solvent under reduced pressure to obtain a rhizoma ligustici wallichii root extract;

the extraction method of the angelica root extract comprises the following steps: putting the crushed angelica root into 6-8 times of 70-85% ethanol for ultrasonic extraction for 60-120 min, collecting the extracting solution, and removing the solvent under reduced pressure to obtain an angelica root extract;

the extraction method of the Shengma Magen extract comprises the following steps: putting the crushed cimicifugae foetidae and the crushed material into 5-10 times of 65-80% ethanol for ultrasonic extraction, wherein the ultrasonic extraction time is 60-120 min, collecting an extracting solution, and removing a solvent under reduced pressure to obtain a cimicifugae rhizoma extract;

the extraction method of the radix sileris extract comprises the following steps: putting the radix sileris crushed material into 65-85% ethanol by volume fraction for ultrasonic extraction for 30-90 min, collecting the extracting solution, repeating ultrasonic extraction for 2-3 times, and removing the solvent under reduced pressure to obtain the radix sileris extract;

the extraction method of the lycium barbarum root extract comprises the following steps: and (3) putting the crushed wolfberry root into 65-85% ethanol for ultrasonic treatment for 30-60 min, collecting an extracting solution, repeating the ultrasonic treatment for 2-3 times, and removing the solvent under reduced pressure to obtain the wolfberry root extract.

In a third aspect, the invention provides an application of the Chinese herbal medicine compound extract prepared by the preparation method of the Chinese herbal medicine compound extract or the Chinese herbal medicine compound extract in preparing anti-inflammatory and antipruritic medicines or cosmetics.

In a fourth aspect, the invention provides a medicine or a cosmetic comprising the Chinese herbal medicine compound extract prepared by the preparation method of the Chinese herbal medicine compound extract or the Chinese herbal medicine compound extract.

Compared with the prior art, the invention has the following beneficial effects:

(1) the Chinese herbal medicine compound extract provided by the invention comprises a ligusticum wallichii root extract, a angelica sinensis root extract, a cimicifuga root extract, a divaricate saposhnikovia root extract, a Chinese wolfberry root extract, water and optional auxiliary materials; the components are all from natural plants, contain various active ingredients, are healthy and safe, relieve the defect that the skin allergy problem is easy to relapse in the traditional western medicine solution, and have the advantages of simple formula, low toxic and side effects and good anti-inflammatory and itching relieving effects.

(2) The invention provides a preparation method of the Chinese herbal medicine compound extract, which has the advantages of simple process, high extraction rate, low cost and short time.

Drawings

FIG. 1 is a schematic diagram of the detection of lymphocytes in the group of compound Chinese herbal medicine extracts of example 1 by electron microscopy for delayed allergic reaction of lymphocytes in the invention;

FIG. 2 is an electron microscope detection diagram of lymphocyte in the compound Chinese herbal medicine extract group of example 2 for detecting delayed allergic reaction lymphocytes according to the present invention;

FIG. 3 is an electron microscope detection diagram of lymphocyte in the compound Chinese herbal medicine extract group of example 3 for detecting delayed allergic reaction lymphocytes according to the present invention;

FIG. 4 is an electron microscope detection diagram of lymphocyte in the compound Chinese herbal medicine extract group of example 4 for detecting delayed allergic reaction lymphocytes according to the present invention;

FIG. 5 is an electron microscope detection diagram of lymphocyte in the group of compound Chinese herbal medicine extract of example 5 for detecting delayed allergic reaction lymphocytes according to the present invention;

FIG. 6 is a schematic diagram of the electron microscope for detecting normal lymphocytes by delayed allergy lymphocytes according to the present invention;

FIG. 7 is a schematic diagram of the electron microscope detection of lymphocytes in the model group according to the embodiment of the invention;

FIG. 8 is a schematic diagram of the detection of lymphocyte in hydrocortisone cream group according to the detection example of delayed allergic reaction lymphocyte in the invention.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

According to a first aspect of the invention, a Chinese herbal medicine compound extract is provided, which comprises the following components: ligusticum wallichii root extract, angelica sinensis root extract, Shengma yuen extract, divaricate saposhnikovia root extract, medlar root extract, water and optional auxiliary materials.

Ligusticum wallichii is the rhizome of a plant of ligusticum of umbelliferae, is a common Chinese herbal medicine, contains multiple active ingredients, and is widely applied to medicines in cardiovascular system, nervous system, respiratory system and digestive system. The rhizoma Ligustici Chuanxiong root extract is extract of rhizoma Ligustici Chuanxiong root.

The radix Angelicae sinensis is derived from root of Angelica sinensis (oliv.) Diels of Umbelliferae, and has effects of replenishing blood, promoting blood circulation, regulating menstruation, relieving pain, loosening bowel, and relieving constipation. The active ingredients in angelica are more in variety, for example: butenyl phthalide, ligustilide, guaiacol, and organic acid; the radix Angelicae sinensis extract is radix Angelicae sinensis extract.

Cimicifugae foetidae, another name: longan root, herba Apocyni Veneti, and root of Grodonta Gredler are rhizome of Cimicifuga trifoliata, Cimicifuga dahurica or Cimicifuga foetida of Cimicifuga of Ranunculaceae. Is a widely applied Chinese herbal medicine, and is commonly used as a medicine component in the aspects of reducing blood pressure, tranquilizing, resisting convulsion, inhibiting nucleoside operation, resisting virus, reducing blood fat, protecting liver, resisting osteoporosis, relieving fever and pain, resisting ulcer and the like; the radix cimicifugae extract is radix cimicifugae extract, preferably radix cimicifugae extract.

Radix Saposhnikoviae is an Umbelliferae plant radix Saposhnikoviae, and dry root of plant without stem is used as Chinese herbal medicine component for relieving exterior syndrome, dispelling pathogenic wind, eliminating dampness, and relieving spasm, and can be used for treating common cold, headache, rheumatalgia, rubella pruritus and tetanus; the radix Saposhnikoviae root extract is extract of radix Saposhnikoviae root.

Lycium chinense belongs to solanaceae plants, and fruits and roots can be used as medicines and are common traditional Chinese medicine components. The medlar has the functions of promoting immunity, resisting aging, eliminating free radicals, resisting radiation and the like; the Lycii radix extract is root extract of Lycii Frutus.

Adjuvants are optionally added, which are generally auxiliary components, such as pharmaceutical and cosmetic adjuvants, typical but not limiting examples being: sweetening agents, flavoring agents, mucilage agents, antioxidants, surfactants, humectants, preservatives, or the like.

Specific Chinese herbal medicines are selected for compounding, so that better anti-inflammatory and itching relieving effects are achieved, and the Chinese herbal medicine extract is natural in components and free of toxic and side effects, so that the body function can be fundamentally improved, the effects of treating the root cause and preventing the symptoms from easily recurring are achieved.

In a preferred embodiment, the Chinese herbal medicine compound extract comprises the following components in percentage by mass: 1-5% of ligusticum wallichii root extract, 1-5% of angelica sinensis root extract, 1-5% of radix cimicifugae extract, 1-5% of radix sileris extract, 1-5% of lycium barbarum root extract, 0.1-1% of auxiliary material and the balance of water.

Typical but non-limiting contents of cnidium officinale root extract are for example: 1%, 2%, 3%, 4% or 5%;

typical but non-limiting angelica root extract contents are for example: 1%, 2%, 3%, 4% or 5%;

typical but non-limiting amounts of the extract of the kenaf root are for example: 1%, 2%, 3%, 4% or 5%;

typical but non-limiting amounts of the extract of ledebouriella root are for example: 1%, 2%, 3%, 4% or 5%;

typical but non-limiting boxthorn root extract contents are for example: 1%, 2%, 3%, 4% or 5%;

typical but non-limiting amounts of excipients are for example: 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1%.

The term "comprising" as used herein means that it may include other components in addition to the components described herein, which impart different properties to the herbal compound extract. In addition, the term "comprising" as used herein may be replaced by "being" or "consisting of … …" as closed.

It should be noted that the balance of water refers to the balance of water in the components of the Chinese herbal medicine compound extract except for the ligusticum wallichii root extract, the angelica sinensis root extract, the cimicifuga foetida root extract, the saposhnikovia divaricata root extract, the lycium barbarum root extract, the auxiliary materials and optionally other components, and the sum of the mass percentages of the water, the ligusticum wallichii root extract, the angelica sinensis root extract, the cimicifuga foetida root extract, the saposhnikovia divaricata root extract, the lycium barbarum root extract, the auxiliary materials and optionally other components is 100%.

The component proportion of each Chinese herbal medicine extract is determined, so that better cooperation and synergism can be achieved.

In a preferred embodiment, the auxiliary material comprises: one or more of sweetener, aromatic, mucilage, antioxidant, surfactant, humectant or antiseptic.

Typical but non-limiting sweeteners are for example: sucrose, fructose or glucose.

Typical but non-limiting fragrances are, for example: lemon, peppermint oil or essence;

typical but non-limiting mucilages are for example: sodium alginate, acacia, gelatin, methylcellulose, or sodium carboxymethylcellulose;

typical but non-limiting antioxidants are for example: vitamin a, vitamin C, vitamin D, vitamin E, alpha-lipoic acid or coenzyme Q10;

typical but non-limiting surfactants are for example: phospholipids, choline, protein or sodium stearate;

typical but non-limiting humectants are for example: glycerol, collagen, hyaluronic acid or amino acids;

typical but non-limiting preservatives are for example: sodium benzoate or potassium sorbate.

By adding auxiliary materials, the Chinese herbal compound extract can be adjusted according to actual needs, such as but not limited to: regulating smell, properties and quality guarantee period.

In a preferred embodiment, a typical herbal compound extract comprises the following components in percentage by mass: 1-5% of ligusticum wallichii root extract, 1-5% of angelica sinensis root extract, 1-5% of dahurian angelica root extract, 1-5% of divaricate saposhnikovia root extract, 1-5% of lycium barbarum root extract, 0.3-0.6% of sodium benzoate, 0.1-0.3% of potassium sorbate and the balance of water.

The typical Chinese herbal medicine compound extract has reasonable formula and good effect, and can obtain excellent anti-inflammatory and itching relieving effects.

According to a second aspect of the present invention, there is provided a method for preparing the above herbal compound extract, comprising the steps of:

mixing rhizoma Ligustici Chuanxiong root extract, radix Angelicae sinensis extract, radix Helicteris extract, radix Saposhnikoviae extract, fructus Lycii extract, water and optional adjuvants uniformly to obtain Chinese herbal medicine compound extract.

The extracts of the Chinese herbal medicines can be obtained by various methods, and typical but non-limiting extraction methods are as follows: ultrasonic extraction, reflux extraction, etc. Through mixing various components, the Chinese herbal medicine compound extract is obtained, contains various active ingredients and has the function of synergy.

In a preferred embodiment, the extract of the root of chuanxiong rhizome is obtained by extracting a crushed material of the root of chuanxiong rhizome in 65-85% ethanol by volume fraction;

typical but non-limiting ethanol concentrations are for example: 65%, 70%, 75%, 80% or 85%;

typical but non-limiting ethanol concentrations are for example: 70%, 75%, 80% or 85%;

the ethanol is preferably used for extraction, the cost is low, the extraction efficiency is high, the application range is wide, and the components in different Chinese herbal medicine raw materials are different, so the ethanol with different concentration ranges is used for extracting the Chinese herbal medicine.

In a preferred embodiment, the extraction method of the ligusticum wallichii root extract comprises the following steps: soaking the crushed rhizoma ligustici wallichii root in 65-85% ethanol for 30-60 min, extracting at 75-85 ℃ for 20-60 min, repeatedly extracting for 2-3 times, and removing the solvent under reduced pressure to obtain a rhizoma ligustici wallichii root extract;

typical but non-limiting immersion times are for example: 30min, 40min, 50min or 60 min; typical but non-limiting water bath times are for example: 20min, 30min, 40min, 50min or 60 min;

typical but non-limiting times of repeated extraction of the dregs are for example: 2 times or 3 times.

In a preferred embodiment, the method for extracting the angelica root extract comprises the following steps: putting the crushed angelica root into 6-8 times of 70-85% ethanol for ultrasonic extraction for 60-120 min, collecting the extracting solution, and removing the solvent under reduced pressure to obtain an angelica root extract;

the amount of the ethanol added is 6-8 times of the amount of the angelica root added.

Typical but non-limiting examples of the weight ratio of the ethanol solution are: 6 times, 7 times or 8 times;

typical but non-limiting ethanol ultrasound extraction times are for example: 60min, 70min, 80min,

90min, 100min, 110min or 120 min.

In a preferred embodiment, the method for extracting the Shengma Makino extract comprises the following steps: putting the crushed cimicifugae foetidae and the crushed material into 5-10 times of 65-80% ethanol for ultrasonic extraction for 60-120 min, collecting the extracting solution, and removing the solvent under reduced pressure to obtain a cimicifugae rhizoma extract;

the amount of the ethanol added is 6 to 8 times of the amount of the added Shengma root.

Typical but non-limiting multiples of ethanol content are, for example: 5 times, 6 times, 7 times, 8 times, 9 times, or 10 times;

in a preferred embodiment, the extraction method of the saposhnikovia divaricata root extract comprises the following steps: putting the radix sileris crushed material into 65-85% ethanol by volume fraction for ultrasonic extraction for 30-90 min, collecting the extracting solution, repeating ultrasonic extraction for 2-3 times, and removing the solvent under reduced pressure to obtain the radix sileris extract;

typical but non-limiting ultrasound times are: 30min, 40min, 50min, 60min, 70min, 80min or 90 min.

Typical but non-limiting ultrasound repetition times are: 2 times or 3 times.

In a preferred embodiment, the method for extracting the lycium barbarum root extract comprises the following steps: putting the crushed wolfberry root into 65-85% ethanol, performing ultrasonic treatment for 30-60 min, collecting an extracting solution, repeating the ultrasonic treatment for 2-3 times, and removing the solvent under reduced pressure to obtain a wolfberry root extract;

typical but non-limiting ultrasound times are: 30min, 40min, 50min or 60 min.

Typical but non-limiting ultrasound repetition times are: 2 times or 3 times.

Mixing the extracts, adding antiseptic and water, and aseptic packaging.

The selection of the solvent, the extraction temperature, the extraction method, the extraction time and the extraction repetition times in the extraction mode are correspondingly optimized according to different Chinese herbal medicines and extracted active ingredients, so that higher extraction efficiency can be achieved, the raw material cost is reduced, higher extraction efficiency is obtained, the extraction time is shortened, the extraction steps are simplified, the extraction cost is reduced, the traditional Chinese medicines are effectively extracted, excessive ingredients are prevented from being remained in medicine residues, and the longer quality guarantee period can be obtained by adding the preservative and carrying out sterile filling.

According to the third aspect of the invention, the application of the Chinese herbal medicine compound extract prepared by the preparation method of the Chinese herbal medicine compound extract or the Chinese herbal medicine compound extract in preparing anti-inflammatory and antipruritic medicines or cosmetics is provided.

The Chinese herbal medicine compound extract can be used for preparing medicines or cosmetics with small toxic and side effects, anti-inflammatory and itching relieving effects and good safety.

According to a fourth aspect of the present invention, there is provided a pharmaceutical or cosmetic comprising the herbal compound extract or the herbal compound extract prepared by the method for preparing the herbal compound extract.

The medicine or the cosmetic has the same advantages as the compound Chinese herbal medicine extract or the Chinese herbal medicine compound extract prepared by the preparation method of the compound Chinese herbal medicine extract, and has the advantages of small toxic and side effects, good safety, good anti-inflammatory and itching-relieving effects on skin and difficult recurrence.

For further understanding of the present invention, the method and effects of the present invention will be described in further detail with reference to specific examples. All the raw materials related to the invention can be obtained commercially.

In the examples and comparative examples, the chuanxiong rhizome extract, the angelica root extract, the Shengma Magen root extract, the ledebouriella root extract and the lycium barbarum root extract were prepared by the following methods, respectively:

the extraction method of the ligusticum wallichii root extract comprises the following steps: soaking 1kg of rhizoma Ligustici Chuanxiong crushed material in 70% ethanol for 120min, extracting with 80 deg.C water bath, repeating the extraction for 3 times, and removing solvent under reduced pressure to obtain rhizoma Ligustici Chuanxiong extract;

the extraction method of the angelica root extract comprises the following steps: extracting crushed angelica root 1kg in 70% ethanol 8 times the volume of the crushed angelica root for 120min, collecting the extract, and removing the solvent under reduced pressure to obtain the angelica root extract;

the extraction method of the Shengma Magen extract comprises the following steps: extracting 1kg of rhizoma cimicifugae and crushed material with 5 times of 70% ethanol for 120min, collecting extractive solution, and removing solvent under reduced pressure to obtain radix cimicifugae extract;

the extraction method of the radix sileris extract comprises the following steps: ultrasonic extracting 1kg of radix Saposhnikoviae crushed material in 70% ethanol for 60min, collecting extractive solution, repeating ultrasonic extraction for 3 times, and removing solvent under reduced pressure to obtain radix Saposhnikoviae extract;

the extraction method of the lycium barbarum root extract comprises the following steps: taking 1kg of crushed product of Lycii Frutus root, placing in 70% ethanol, performing ultrasonic treatment for 60min, collecting extractive solution, repeating ultrasonic treatment for 3 times, and removing solvent under reduced pressure to obtain Lycii Frutus root extract;

example 1

Respectively weighing and mixing 3g of ligusticum wallichii root extract, 3g of angelica sinensis root extract, 3g of dahurian angelica root extract, 4g of divaricate saposhnikovia root extract, 4g of medlar root extract, 0.5g of sodium benzoate and 0.2g of potassium sorbate, and adding water to the final volume of 100ml to obtain the Chinese herbal medicine compound extract.

Example 2

Respectively weighing and mixing 3g of ligusticum wallichii root extract, 3g of angelica sinensis root extract, 3g of dahurian angelica root extract, 3g of divaricate saposhnikovia root extract, 4g of medlar root extract, 0.5g of sodium benzoate and 0.2g of potassium sorbate, and adding water to the final volume of 100ml to obtain the Chinese herbal medicine compound extract.

Example 3

Weighing rhizoma Ligustici Chuanxiong root extract 1g, radix Angelicae sinensis extract 3g, radix Cistanchis herba extract 3g, radix Saposhnikoviae extract 4g, fructus Lycii root extract 1g, sodium benzoate 0.4g, and potassium sorbate 0.3g respectively, mixing, and adding water to final volume of 100ml to obtain Chinese herbal compound extract.

Example 4

Weighing rhizoma Ligustici Chuanxiong root extract 2g, radix Angelicae sinensis extract 3g, radix Cistanchis herba extract 3g, radix Saposhnikoviae extract 3g, fructus Lycii root extract 3g, sodium benzoate 0.5g, and potassium sorbate 0.2g respectively, mixing, and adding water to final volume of 100ml to obtain Chinese herbal compound extract.

Example 5

Respectively weighing and mixing 3g of ligusticum wallichii root extract, 3g of angelica sinensis root extract, 1g of dahurian angelica root extract, 4g of divaricate saposhnikovia root extract, 4g of medlar root extract, 0.5g of sodium benzoate and 0.2g of potassium sorbate, and adding water to the final volume of 100ml to obtain the Chinese herbal medicine compound extract.

Comparative example 1

Respectively weighing 3g of angelica root extract, 3g of Xingan Shengmao root extract, 4g of radix sileris root extract, 4g of medlar root extract, 0.5g of sodium benzoate and 0.2g of potassium sorbate, mixing, and adding water to the final volume of 100ml to obtain the Chinese herbal medicine compound extract.

Comparative example 2

Respectively weighing and mixing 3g of ligusticum wallichii root extract, 3g of dahurian angelica root extract, 4g of divaricate saposhnikovia root extract, 4g of barbary wolfberry root extract, 0.5g of sodium benzoate and 0.2g of potassium sorbate, and adding water to the mixture until the final volume is 100ml to obtain the Chinese herbal medicine compound extract.

Comparative example 3

Weighing rhizoma Ligustici Chuanxiong root extract 3g, radix Angelicae sinensis extract 3g, radix Saposhnikoviae extract 4g, fructus Lycii extract 4g, sodium benzoate 0.5g, and potassium sorbate 0.2g respectively, mixing, and adding water to final volume of 100ml to obtain Chinese herbal compound extract.

Comparative example 4

Weighing rhizoma Ligustici Chuanxiong root extract 3g, radix Angelicae sinensis extract 3g, radix Cistanchis herba extract 3g, fructus Lycii root extract 4g, sodium benzoate 0.5g, and potassium sorbate 0.2g respectively, mixing, and adding water to final volume of 100ml to obtain Chinese herbal compound extract.

Comparative example 5

Respectively weighing and mixing 3g of ligusticum wallichii root extract, 3g of angelica sinensis root extract, 3g of dahurian angelica root extract, 4g of divaricate saposhnikovia root extract, 0.5g of sodium benzoate and 0.2g of potassium sorbate, and adding water to the mixture until the final volume is 100ml to obtain the Chinese herbal medicine compound extract.

Comparative example 6

Weighing rhizoma Ligustici Chuanxiong root extract 1g, radix Angelicae sinensis extract 0.5g, radix Cistanchis extract 3g, radix Saposhnikoviae extract 4g, fructus Lycii root extract 5g, sodium benzoate 0.5g, and potassium sorbate 0.2g respectively, mixing, and adding water to final volume of 100ml to obtain Chinese herbal compound extract.

Test example 1 anti-inflammatory test

Experimental animals:

ICR mice, weight 18-22g, male and female, provided by Shanghai laboratory animal center, quality certification: no.34000200000976, 0010424.

Experimental materials:

the Chinese herbal medicine compound extracts used in the experiment are respectively from example 1, example 2, example 3,

Example 4, example 5, comparative example 1, comparative example 2, comparative example 3, comparative example 4, comparative example 5, comparative example 6.

Medicine preparation: hydrocortisone cream, manufactured by tianjin jinyao pharmaceutical ltd, lot number: 15090302.

grouping experiments:

control group: is blank control, no experimental treatment group was performed;

model group: inducing an inflammatory treatment, but not administering a drug treatment;

drug group: inducing inflammation treatment, and treating with hydrocortisone ointment;

example 1 group: inducing inflammation treatment, applying the Chinese herbal medicine compound extract obtained in the example 1;

example 2 group: inducing inflammation treatment, applying the Chinese herbal medicine compound extract obtained in the example 2;

example 3 group: inducing inflammation treatment, applying the Chinese herbal medicine compound extract obtained in the example 3;

example 4 group: inducing inflammation treatment, applying the Chinese herbal medicine compound extract obtained in the example 4;

example 5 group: inducing inflammation treatment, applying the Chinese herbal medicine compound extract obtained in the example 5;

comparative example 1 group: inducing inflammation treatment, applying the Chinese herbal medicine compound extract obtained in the comparative example 1;

comparative example 2 group: inducing inflammation treatment, applying the Chinese herbal medicine compound extract obtained in the comparative example 2;

comparative example 3 group: inducing inflammation treatment, applying the Chinese herbal medicine compound extract obtained in the comparative example 3;

comparative example 4 group: inducing inflammation treatment, applying the Chinese herbal medicine compound extract obtained in the comparative example 4;

comparative example 5 group: inducing inflammation treatment, applying the Chinese herbal medicine compound extract obtained in the comparative example 5;

comparative example 6 group: inducing inflammation treatment, applying the Chinese herbal medicine compound extract obtained in the comparative example 6; the experimental method comprises the following steps:

1. delayed allergic reaction experiment of mice

140 ICR mice were divided into 10 animals per weight for each of the control group, model group, drug group (hydrocortisone cream group), example 1 group, example 2 group, example 3 group, example 4 group, example 5 group, comparative example 1 group, comparative example 2 group, comparative example 3 group, comparative example 4 group, comparative example 5 group, comparative example 6 group. Performing dermatitis induction: the back of each group of mice was dehaired, and the area was about 2cm × 2cm, 100 μ l of 7% DNCB acetone solution was applied to the back of the mice by a sample applicator, and 20 μ l of 0.1% DNCB solution was applied to the right ear of the mice 5 days after sensitization, and the challenge was performed 1 time every 3 days for 5 times.

The topical application is administered once a day, 0.2 ml/time, in the morning and evening, for 14 days. When in administration, 100 mul and 20 mul of each component solution and solvent are respectively taken and respectively coated on the back sensitization area and the inner and outer surfaces of the right ear. At 48h after each challenge, mice were observed for symptoms of dorsal skin inflammation and photographed. 12h after the last dose, mice were sacrificed by cervical amputation and treated as follows: firstly, selecting 3 different fixed parts of the back skin of the mouse, punching the fixed parts by using a metal puncher with the thickness of 8mm, immediately measuring the thickness by using an external micrometer after removing subcutaneous adipose tissues, and weighing by using an analytical balance. Fixing the back skin specimen with 4% formaldehyde, embedding in paraffin, slicing, HE staining, and counting lymphocytes.

2. Experiment for mouse auricle swelling caused by xylene

Taking 140 mice with the weight of 18-22g and half of each sex, randomly dividing the mice into 14 groups: control group, model group, drug group (hydrocortisone cream group), example 1 group, example 2 group, example 3 group, example 4 group, example 5 group, comparative example 1 group, comparative example 2 group, comparative example 3 group, comparative example 4 group, comparative example 5 group, comparative example 6 group, each group containing 10 animals. The right ear of the mouse is coated with each group of corresponding medicines, 0.2ml of each group of medicines is coated once a day for 3 days continuously, 30min after the last administration, 100% dimethylbenzene inflammation-causing liquid is coated on the front and back surfaces of the right ear of the mouse, the left ear is not treated, the animal is killed after 4 hours, two ears are cut off immediately, the ear at the same part is taken by an 8mm puncher, the ear is weighed on an analytical balance, the swelling degree is determined by subtracting the weight of the left ear from the weight of the right ear, and the swelling rate is determined by (the weight of the right ear-the weight of the left ear)/the weight of the left ear multiplied by 100%.

The experimental results are as follows:

1. effect of each drug tested on thickness and weight of the skin on the back of mice

In the experiment, animals died in each of the groups except the control group and example 4, and the specific number is shown in the following table. Mouse delayed allergy experiment shows that the influence of each tested drug on the thickness and weight of the back skin of a mouse is shown in the specific results in table 1.

TABLE 1 delayed allergic response in mice the effect of each test drug on the thickness and weight of the skin on the back of the mice

Note: compared with the control group, the compound of the formula,^**p is less than 0.01; in comparison with the set of models,^#P＜0.05，^##P＜0.01。

2. effect of Each test drug on the number of lymphocytes in the dorsal skin of mice

After the skin of each group of animals was HE-stained, the number of lymphocytes was observed under a light microscope. The specific results of the test drugs on the number of lymphocytes on the back skin of the mice in the delayed allergic reaction experiments of the mice are shown in Table 2, the lymphocyte electron microscope pictures (HE stained by 400 times) of examples 1-5 are shown in the attached figures 1-5, and the lymphocyte electron microscope pictures of a control group, a model group and a drug group (hydrocortisone cream group) are shown in the attached figures 6-8.

TABLE 2 delayed allergic response in mice the Effect of each test drug on the number of lymphocytes in the skin on the back of mice

Note: in comparison with the normal group,^**p is less than 0.01; in comparison with the set of models,^#P＜0.05，^##P＜0.01。

3. influence of each test drug on mouse auricle swelling experiment caused by paraxylene

Xylene-induced mouse auricle swelling experiment the specific results of the test drugs on the influence of xylene-induced mouse auricle swelling are shown in table 3.

TABLE 3 Effect of each test agent on swelling of auricle in mice induced by xylene

Note that, compared with the normal group,^**p is less than O, 01; in comparison with the set of models,^#P＜0,05。

as can be seen from the test results in table 1, there was a significant increase in skin thickness and weight on the back of the mice compared to the normal group; compared with the model group, the skin thickness of the back of the mouse can be reduced in the groups of example 1, example 2, example 3, example 4, example 5, comparative example 1, comparative example 2, comparative example 3, comparative example 4, comparative example 5 and comparative example 6, and the effects of example 1, example 2 and example 4 are more obvious; the weight of the skin on the back of the mouse can be obviously reduced by the examples 1, 2 and 4, wherein the effect of the example 1 is the most obvious, the examples 5 and 3 are slightly poorer than other examples, but the better effect can be achieved, and the effects of the comparative examples 1, 2, 3, 4, 5 and 6 are not good.

As can be seen from the results of the skin lymphocyte count test in Table 2, the skin lymphocyte count of the animals in the model group was significantly increased compared with that in the normal group; compared with the model group, the examples 1, 4, 2 and 5 can obviously reduce the number of the lymphocytes on the back skin of the mice, and the example 3 is poorer, wherein the effects of the examples 1 and 4 are more obvious.

As can be seen from the test results in table 3, the swelling rate of the auricle of the mouse in the model group is significantly increased compared with the normal group, and the swelling rate of the auricle of the mouse is significantly decreased in examples 1 and 4, and the swelling rate of examples 2, 3 and 5 is also decreased to a certain extent in comparison with the model group, which is better than that in the comparison examples, and the swelling rate reduction effect in comparison examples 1 to 6 is poorer.

As can be seen from tables 1 to 3, examples 1 to 5 achieved better anti-inflammatory effects than comparative examples 1 to 6 as a whole, and the present invention achieved better anti-inflammatory effects using purely natural ingredients.

Test example 2 antipruritic test

Experimental animals:

ICR mouse, weight 18 ~ 22g, the sex is used concurrently, is provided by Shanghai laboratory animal center, and quality certification: 0010424.

white guinea pig, weight 250-: 0032052. experimental materials:

the Chinese herbal medicine compound extracts used in the experiment are respectively from an example 1 group, an example 2 group, an example 3 group, an example 4 group, an example 5 group, a comparative example 1 group and a comparative example 2 group, a comparative example 3 group, a comparative example 4 group, a comparative example 5 group and a comparative example 6 group.

Medicine preparation: triamcinolone acetonide acetate urea ointment is produced by Zhejiang dean pharmaceutical Co., Ltd, and has the following batch numbers: 1603501A.

Histamine phosphate, manufactured by michelin biochemistry ltd, shanghai, under batch number: C10052437. grouping experiments:

control group: as a control, pruritus treatment was induced, and distilled water was applied;

drug group: inducing pruritus treatment and applying drug treatment, wherein the drug is triamcinolone acetonide acetate urea ointment;

example 1 group: inducing pruritus treatment by applying the Chinese herbal medicine compound extract obtained in example 1;

example 2 group: inducing pruritus treatment by applying the Chinese herbal medicine compound extract obtained in the example 2;

example 3 group: inducing pruritus treatment by applying the Chinese herbal medicine compound extract obtained in example 3;

example 4 group: inducing pruritus treatment by applying the Chinese herbal medicine compound extract obtained in the example 4;

example 5 group: inducing pruritus treatment by applying the Chinese herbal medicine compound extract obtained in example 5;

comparative example 1 group: inducing pruritus treatment, and applying the Chinese herbal medicine compound extract obtained in the comparative example 1;

comparative example 2 group: inducing pruritus treatment, and applying the Chinese herbal medicine compound extract obtained in the comparative example 2;

comparative example 3 group: inducing pruritus treatment, and applying the Chinese herbal medicine compound extract obtained in the comparative example 3;

comparative example 4 group: inducing pruritus treatment, and applying the Chinese herbal medicine compound extract obtained in the comparative example 4;

comparative example 5 group: inducing pruritus treatment, and applying the Chinese herbal medicine compound extract obtained in the comparative example 5;

comparative example 6 group: inducing pruritus treatment, and applying the Chinese herbal medicine compound extract obtained in the comparative example 6; the experimental method comprises the following steps:

1. inhibition effect of each test drug on mouse itching caused by dextran

Taking 130 mice with the weight of 18-22g and half of each sex, and randomly dividing the mice into 14 groups according to the sex and the weight: the group of example 1, the group of example 2, the group of example 3, the group of example 4, the group of example 5, the group of comparative example 1, the group of comparative example 2, the group of comparative example 3, the group of comparative example 4, the group of comparative example 5, the group of comparative example 6, the group of drugs (triamcinolone acetonide acetate urea ointment group) and the distilled water control group were subjected to experiments in which the back hairs of the animals were removed with 1% sodium sulfide and the area of the skin area of the removed hairs was 2cm × 2cm, and then subjected to the experiments after 2 days. During the experiment, the hair removal area on the back of each group of animals is respectively coated with corresponding medicines (0.2 ml/animal). The continuous 5 days, 30min after the last administration, dextran 0.95mg/kg is injected into tail vein of each mouse. Taking the positions of the front paw scratching head, the back paw scratching trunk and the whole body bitten by mouth of the mouse as itching indications, recording the itching times of the mouse within 30 minutes, and comparing the difference between groups.

2. Inhibition of histamine-induced local skin itching in guinea pigs with each test agent

130 white guinea pigs weighing 250-. The experimental groups are respectively smeared with the tested medicine 4 times, 2 times daily, 0.025mL each time, and the area is about 1.1cm²Wrapping with gauze after natural film formation, fixing with adhesive tape, and topically applying the extract to the administration group with a thickness of 1.2cm²Then bandaged and fixed. On day 3, gently rubbing skin of the depilated area of the back of the right foot with fine abrasive paper to make it reddish but not bleed, applying 0.2mL of the tested drug to the wound surface, gently removing the tested drug after 40min, and applying 0.03mL of histamine to the back of each guinea pig foot from low concentration to high concentration at intervals of 3min, wherein the histamine concentration is 1.0 × 10^-4、1.5×10^-4、2.0×10^-4、2.5×10^-4、3.0×10^-4、3.5×10^-4、4.0×10^-4. When guinea pigs appearWhen the itching reaction is carried out, namely the wound surface part of the right hind paw is licked (or the wound surface part is smeared until the itching reaction does not appear at the highest concentration), the histamine amount given by each guinea pig is accumulated at the moment, namely the histamine tolerance amount of the guinea pig, and the histamine tolerance amount is used as an index to carry out t-test statistical treatment to evaluate the itching relieving effect of the medicine.

The experimental results are as follows:

1. inhibition effect of each test drug on mouse itching caused by dextran

The specific results are shown in Table 4.

TABLE 4 inhibitory Effect of each test drug on mouse itching caused by dextran

Note: in comparison with the set of models,^##P<0.01。

The specific results are shown in Table 5.

TABLE 5 inhibitory Effect of each test agent on local cutaneous pruritus in Guinea pig caused by Histamine

Note: in comparison with the set of models,^##P<0.01。

as can be seen from the test results in Table 4, example 1 had a significant effect of inhibiting the itching of mice as compared with the control group. The effects of the embodiment 2, the embodiment 3 and the embodiment 5 are not as good as the effects of the embodiment 1 and the embodiment 4, wherein the effect of the embodiment 1 is better.

As can be seen from the test results in Table 5, example 1 significantly inhibited the itching reaction in guinea pigs, and examples 2, 3 and 5 were less effective than examples 1 and 4, as compared to the negative control, in which example 4 was not as effective as example 1, example 3 was not as effective, and comparative examples 1 to 6 were not as effective.

As can be seen from the test results in tables 4 and 5, examples 1 to 5 achieved better antipruritic effects than comparative examples 1 to 6 in general, and the present invention achieved better antipruritic effects using pure natural ingredients.

While particular embodiments of the present invention have been illustrated and described, it would be obvious that various other changes and modifications can be made without departing from the spirit and scope of the invention. It is therefore intended to cover in the appended claims all such changes and modifications that are within the scope of this invention.

Claims

1. The Chinese herbal medicine compound extract is characterized by comprising the following components: rhizoma Ligustici Chuanxiong root extract, radix Angelicae sinensis root extract, radix Helicteris extract, radix Saposhnikoviae root extract, fructus Lycii root extract, water and antiseptic.

2. The Chinese herbal medicine compound extract as claimed in claim 1, which comprises the following components in percentage by mass:

1-5% of ligusticum wallichii root extract, 1-5% of angelica sinensis root extract, 1-5% of radix cimicifugae extract, 1-5% of radix sileris extract, 1-5% of lycium barbarum root extract, 0.1-1% of preservative and the balance of water;

preferably, the Chinese herbal medicine compound extract comprises the following components in percentage by mass: 1-5% of ligusticum wallichii root extract, 2-5% of angelica sinensis root extract, 1-5% of radix cimicifugae extract, 2-5% of radix sileris extract, 1-5% of lycium barbarum root extract, 0.1-1% of preservative and the balance of water;

preferably, the Chinese herbal medicine compound extract comprises the following components in percentage by mass: 2-5% of ligusticum wallichii root extract, 3-5% of angelica sinensis root extract, 2-5% of radix cimicifugae extract, 3-5% of radix sileris extract, 2-5% of lycium barbarum root extract, 0.1-1% of preservative and the balance of water.

3. The herbal compound extract as claimed in claim 2, wherein the preservative comprises sodium benzoate and/or potassium sorbate.

4. The Chinese herbal medicine compound extract as claimed in any one of claims 1 to 3, which comprises the following components in percentage by mass:

1-5% of ligusticum wallichii root extract, 1-5% of angelica sinensis root extract, 1-5% of radix cimicifugae extract, 1-5% of radix sileris extract, 1-5% of lycium barbarum root extract, 0.3-0.6% of sodium benzoate, 0.1-0.3% of potassium sorbate and the balance of water.

5. A method for preparing a herbal compound extract as claimed in any one of claims 1 to 4, comprising the steps of:

mixing rhizoma Ligustici Chuanxiong root extract, radix Angelicae sinensis extract, radix Helicteris extract, radix Saposhnikoviae extract, fructus Lycii extract, water and optional antiseptic uniformly to obtain Chinese herbal medicine compound extract.

6. The preparation method of the Chinese herbal medicine compound extract according to claim 5, characterized in that the chuanxiong rhizome root extract is obtained by extracting chuanxiong rhizome root crushed material in ethanol with the volume fraction of 65-85%;

preferably, the wolfberry root extract is obtained by extracting crushed wolfberry root in 65-85 vol% ethanol.

7. The preparation method of the Chinese herbal medicine compound extract as claimed in claim 6, wherein the extraction method of the ligusticum wallichii root extract comprises the following steps:

soaking the crushed rhizoma ligustici wallichii root in 65-85% ethanol for 30-60 min, extracting at 75-85 ℃ for 20-60 min, repeatedly extracting for 2-3 times, and removing the solvent under reduced pressure to obtain a rhizoma ligustici wallichii root extract;

preferably, the extraction method of the angelica root extract comprises the following steps: putting the crushed angelica root into 6-8 times of 70-85% ethanol for ultrasonic extraction for 60-120 min, collecting the extracting solution, and removing the solvent under reduced pressure to obtain an angelica root extract;

preferably, the extraction method of the Shengma Magen extract comprises the following steps: putting the crushed cimicifugae foetidae and the crushed material into 5-10 times of 65-80% ethanol for ultrasonic extraction, wherein the ultrasonic extraction time is 60-120 min, collecting an extracting solution, and removing a solvent under reduced pressure to obtain a cimicifugae rhizoma extract;

preferably, the extraction method of the radix sileris extract comprises the following steps: putting the radix sileris crushed material into 65-85% ethanol by volume fraction for ultrasonic extraction for 30-90 min, collecting the extracting solution, repeating ultrasonic extraction for 2-3 times, and removing the solvent under reduced pressure to obtain the radix sileris extract;

preferably, the extraction method of the lycium barbarum root extract comprises the following steps: and (3) putting the crushed wolfberry root into 65-85% ethanol for ultrasonic treatment for 30-60 min, collecting an extracting solution, repeating the ultrasonic treatment for 2-3 times, and removing the solvent under reduced pressure to obtain the wolfberry root extract.

8. The use of the herbal compound extract as defined in any one of claims 1 to 7 or the herbal compound extract obtained by the method for preparing the herbal compound extract as defined in any one of claims 6 to 7 in the preparation of anti-inflammatory antipruritic drugs or cosmetics.

9. A pharmaceutical or cosmetic comprising the herbal compound extract according to any one of claims 1 to 7 or the herbal compound extract obtained by the method for preparing the herbal compound extract according to any one of claims 6 to 7.