CN108307932A - A kind of Termitomyces albuminosus with black skin bacteria stick and its microwave sterilization method - Google Patents

A kind of Termitomyces albuminosus with black skin bacteria stick and its microwave sterilization method Download PDF

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Publication number
CN108307932A
CN108307932A CN201810278941.XA CN201810278941A CN108307932A CN 108307932 A CN108307932 A CN 108307932A CN 201810278941 A CN201810278941 A CN 201810278941A CN 108307932 A CN108307932 A CN 108307932A
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China
Prior art keywords
microwave
black skin
termitomyces albuminosus
bacteria stick
skin bacteria
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Pending
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CN201810278941.XA
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Chinese (zh)
Inventor
段振华
唐小闲
刘艳
何青石
赖青松
朱香澔
李定金
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Hezhou Tian De Star Agricultural And Forestry Investment Development Co Ltd
Hezhou University
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Hezhou Tian De Star Agricultural And Forestry Investment Development Co Ltd
Hezhou University
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Priority to CN201810278941.XA priority Critical patent/CN108307932A/en
Publication of CN108307932A publication Critical patent/CN108307932A/en
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Abstract

The invention discloses a kind of Termitomyces albuminosus with black skin bacteria sticks, are made of cultivating bag compost, and the cultivating bag compost includes following components:Sawdust 30 38%, cotton seed hull 35 43%, rice bran 8 12%, wheat bran 12 16%, gypsum 0.5 2%.The invention also discloses a kind of microwave sterilization methods of Termitomyces albuminosus with black skin bacteria stick.The culture medium prescription of Termitomyces albuminosus with black skin bacteria stick provided by the invention is simple, easily allocates, at low cost.Microwave sterilization method is easy to operate, safe efficient, energy-saving and environmental protection.

Description

A kind of Termitomyces albuminosus with black skin bacteria stick and its microwave sterilization method
Technical field
The present invention relates to a kind of bacteria stick and preparation method thereof more particularly to a kind of Termitomyces albuminosus with black skin bacteria stick and its microwave sterilizations Method.
Background technology
Termitomyces albuminosus with black skin, scientific name are Oudemansiella raphanipes, are the trade name of ovum spore oudemansiella radicata, again Name long root mushroom.The bacterium is with traditional one of the medicinal fungi of very high edible and medical value and China.Because of its fresh and crisp alcohol Perfume, fine and tender taste, unique in taste, delicious flavour, can blood pressure lowering, food and medicament dual-purpose, full of nutrition, and receive the favor of people, and Regard as edible mushroom top grade.
Termitomyces albuminosus with black skin contains the multiple nutritional components such as protein, fat, calcium, phosphorus, iron, the core yellow acid that needed by human body is wanted, It is weak, crowd and the ideal diet good food of the elderly after being ill.It the effect of carrying delicious taste, on monosodium glutamate.Its paddy Propylhomoserin sodium content is higher, thus no matter cooking or steamed or cook soup, flavour is all very delicious, is U.S. rare on dining table Taste delicacies.
Termitomyces albuminosus with black skin, original are wild mushrooms, and existing people use advanced science and technology, and Termitomyces albuminosus with black skin, which is introduced greenhouse, plants Training, realizes the artificial cultivation of Termitomyces albuminosus with black skin, and Termitomyces albuminosus with black skin is made still to maintain its exclusive flavor, cultivated area it is fast Speed, which increases, reduces its market price, and more people is allowed to enjoy this deliciousness.
China's major part Termitomyces albuminosus with black skin uses bacteria stick cultivation technique, the nutrition and life required due to Termitomyces albuminosus with black skin growth State environmental requirement is high, thus Termitomyces albuminosus with black skin bacteria stick culture medium and the manufacturing technology of bacteria stick be to seem very crucial.Except this, bacterium Stick cultivation needs first sterilize to bacteria stick, and the step is equally critically important, and only sterilize thorough bacteria stick, bacterium after inoculation Silk ability healthy growth, to obtain the edible mushroom of good quality and high output.Currently, most of Termitomyces albuminosus with black skin manufacturing enterprise, in bacterium In stick manufacturing process, the formula of culture medium is complicated, and consumptive material is more, and incubation time is long, of high cost;Existing bacteria stick sterilizing is mainly adopted Steam is generated with fuel such as burn wood, coals, it is not thorough to be frequently present of sterilizing in sterilization process for the smoke pollution of environment of generation Bottom, compost is rancid, living contaminants, the problems such as sterilization time is long, mycelia is slow-growing, and then influence Termitomyces albuminosus with black skin growth, Cause edible mushroom low output, poor quality, of high cost.
Invention content
In order to solve the above-mentioned problems of the prior art, it is an object of the present invention to provide a kind of Termitomyces albuminosus with black skin bacterium Stick.
The second purpose of the present invention is to provide the microwave sterilization methods of above-mentioned Termitomyces albuminosus with black skin bacteria stick.
One of the object of the invention is achieved through the following technical solutions:A kind of Termitomyces albuminosus with black skin bacteria stick, by cultivating bag culture Material is made, and the cultivating bag compost includes following components:Sawdust 30-38%, cotton seed hull 35-43%, rice bran 8-12%, wheat bran 12-16%, gypsum 0.5-2%.
As an embodiment of the present invention, the Termitomyces albuminosus with black skin stick is cylindrical, and bacteria stick specification is:Diameter 13- 15cm, high 38-42cm, single bacteria stick weight 1.2-1.6kg.
The two of the object of the invention are achieved through the following technical solutions:A kind of microwave sterilization side of Termitomyces albuminosus with black skin bacteria stick Termitomyces albuminosus with black skin bacteria stick is placed in microwave equipment cavity progress microwave sterilization processing by method, sterilization process include heat temperature raising and 2 stages of constant temperature;Wherein, the microwave power of heat temperature raising is 1.0-2.5kW, and the microwave heating heating-up time is 10-300min;It is permanent The microwave power of temperature is 0-0.5kW, constant temperature time 0-300min.
2 stages of heat temperature raising and constant temperature of the present invention are continuous, and constitute complete sterilization process.
In the present invention, the heat temperature raising indicates that microwave is in heated condition, and constant temperature is run by microwave and stops heating It constitutes.
The constant temperature time is made of microwave run time and microwave heating dwell time, it is preferable that each microwave operation Time is 20 or 60s, and stopping heating time is 40 or 60s.Constant temperature total time of the present invention is preferably 0-200min.
The Termitomyces albuminosus with black skin stick presses 34.5-181.5kg/m3Useful load be placed in microwave equipment cavity.
Compared with the prior art, the present invention has the following advantages:
(1) the cultivating bag culture material formula of Termitomyces albuminosus with black skin bacteria stick provided by the invention is simple, easily allocates, and raw material is cheap, It is at low cost.Manufactured bacteria stick inoculation survival rate is high.It is more existing same using the yield of bacteria stick Termitomyces albuminosus with black skin provided by the invention Class bacteria stick is high, average per bacteria stick 500g, or even can reach 600g or more.
(2) microwave is a kind of frequency electromagnetic waves, and under the action of some strength microwave field, microwave has material microorganism Lethal effect, i.e. fuel factor and non-thermal effect.Microorganism is mainly made of water and protein, and wherein water is polar molecule, moisture Son violent Wen Sheng under the action of electromagnetic field of high frequency, protein receptor heat space structure change or destruction, protein denaturation make bacterium It is difficult to survive and dead.Furthermore microorganism, under strong electromagnetic wave, metabolism and genetic process are blocked, cell membrane and hereditary object Matter is destroyed, to be killed.On the other hand, the non-thermal effect of microwave energy plays Typical physical sterilizing in sterilizing and is not had Some special roles, the polar molecule in organism generates strong rotation in microwave field makes the vegetative cell of microorganism lose Enzyme system in activity or destruction microbial cell, causes the death of microorganism.Microwave energy is penetrated into the inside of medium and material, It is inside and outside to heat simultaneously, have penetration power is strong, heating rate is fast, heating time is short, sterilization is uniform, low power consuming, easy to control, easy care The advantages that.A large amount of sterilization times are thus saved using microwave sterilization, shorten hypha growth cycle;Sterilizing cost is saved; Easy to operate, sterilizing is thorough, and environmental protection does not generate harmful substance;Bacteria stick bacterial contamination rate after microwave sterilization is gone out than conventional vapor Bacterium it is low.
(3) using the yield of the casting skin Termitomyces albuminosus of the bacteria stick after sterilization treatment of the present invention and the yield after conventional vapor sterilizing There is no notable difference.
Specific implementation mode
Embodiment 1
Termitomyces albuminosus with black skin stick is prepared:
Cylindrical Termitomyces albuminosus with black skin bacteria stick is made by cultivating bag compost, cultivating bag compost is composed of the following components:Wood Bits 35%, cotton seed hull 40%, rice bran 9%, wheat bran 15%, gypsum 1%.
Full appearance, unabroken Termitomyces albuminosus with black skin bacteria stick are chosen, by 51.42-68.20kg/m3Useful load be placed in it is micro- In wave device cavity, microwave sterilization cabinet door is shut, and the technological parameter in each stage is set as follows.
The heat temperature raising stage:The microwave power 1.0kW of heat temperature raising, microwave heating heating-up time 170min.
The constant temperature stage:Microwave power is 0.5kW, stops the cycle of heating 60s with microwave operation 60s, this phases-time is 40min。
Microwave sterilization total time is 210min, and the Termitomyces albuminosus with black skin bacteria stick bacterial contamination rate after sterilizing is compared with steam sterilizing It is low.It is taken out after being cooled to room temperature bacteria stick after sterilizing, inoculated and cultured according to a conventional method after 0-3 days, average product 500g/ Stick, maximum output 600g/ sticks.
Embodiment 2
Termitomyces albuminosus with black skin stick makes:
Cylindrical Termitomyces albuminosus with black skin bacteria stick is made by cultivating bag compost, cultivating bag compost is composed of the following components:Wood Bits 37%, cotton seed hull 38.5%, rice bran 10%, wheat bran 13%, gypsum 1.5%.
Full appearance, unabroken Termitomyces albuminosus with black skin bacteria stick are chosen, by 68.56-90.80kg/m3Useful load be placed in it is micro- In wave device cavity, microwave sterilization cabinet door is shut, and the technological parameter in each stage is set as follows.
The heat temperature raising stage:The microwave power 1.5kW of heating, microwave heating heating-up time 150min.
The constant temperature stage:Microwave power is 0.3kW, stops the cycle of heating 40s with microwave operation 20s, this phases-time is 30min。
Microwave sterilization total time is 180min, and the Termitomyces albuminosus with black skin bacteria stick bacterial contamination rate after sterilizing is compared with steam sterilizing It is low.It is taken out after being cooled to room temperature bacteria stick after sterilizing, inoculated and cultured according to a conventional method after 0-3 days, average product 500g/ Stick, maximum output 600g/ sticks.
Embodiment 3
Termitomyces albuminosus with black skin stick makes:
Cylindrical Termitomyces albuminosus with black skin bacteria stick is made by cultivating bag compost, cultivating bag compost is composed of the following components:Wood Bits 32%, cotton seed hull 43%, rice bran 10%, wheat bran 13.65%, gypsum 1.35%.
Full appearance, unabroken Termitomyces albuminosus with black skin bacteria stick are chosen, by 68.92-78.62kg/m3Useful load be placed in it is micro- In wave device cavity, microwave sterilization cabinet door is shut, and the technological parameter in each stage is set in accordance with the following methods.
The heat temperature raising stage:The microwave power 1.5kW of heating, microwave heating heating-up time 60min;The microwave power of heating 1.0kW, microwave heating heating-up time 140min.
The constant temperature stage:Microwave power is 0.5kW, stops the cycle of heating 60s with microwave operation 60s, this phases-time is 40min。
Microwave sterilization total time is 240min, and the Termitomyces albuminosus with black skin bacteria stick bacterial contamination rate after sterilizing is compared with steam sterilizing It is low.It is taken out after being cooled to room temperature bacteria stick after sterilizing, inoculated and cultured according to a conventional method after 0-3 days, average product 500g/ Stick, maximum output 600g/ sticks.
Embodiment 4
Termitomyces albuminosus with black skin stick makes:
Cylindrical Termitomyces albuminosus with black skin bacteria stick is made by cultivating bag compost, cultivating bag compost is composed of the following components:Wood Bits 33%, cotton seed hull 41%, rice bran 11%, wheat bran 14%, gypsum 1%.
Full appearance, unabroken Termitomyces albuminosus with black skin bacteria stick are chosen, by 102.84-110.20kg/m3Useful load be placed in In microwave equipment cavity, microwave sterilization cabinet door is shut, and the technological parameter in each stage is set as follows.
The heat temperature raising stage:The microwave power 2.0kW of heating, microwave heating heating-up time 60min;The microwave power of heating 1.0kW, microwave heating heating-up time 210min.
The constant temperature stage:Microwave power is 0.3kW, stops the cycle of heating 40s with microwave operation 20s, this phases-time is 50min。
Microwave sterilization total time is 320min, and the Termitomyces albuminosus with black skin bacteria stick bacterial contamination rate after sterilizing is compared with steam sterilizing It is low.It is taken out after being cooled to room temperature bacteria stick after sterilizing, inoculated and cultured according to a conventional method after 0-3 days, average product 500g/ Stick, maximum output 600g/ sticks.
For those skilled in the art, technical solution that can be as described above and design are made other each The corresponding change of kind and deformation, and all these changes and deformation should all belong to the protection model of the claims in the present invention Within enclosing.

Claims (7)

1. a kind of Termitomyces albuminosus with black skin bacteria stick, which is characterized in that be made of cultivating bag compost, the cultivating bag compost include with Lower component:Sawdust 30-38%, cotton seed hull 35-43%, rice bran 8-12%, wheat bran 12-16%, gypsum 0.5-2%.
2. Termitomyces albuminosus with black skin bacteria stick according to claim 1, which is characterized in that the Termitomyces albuminosus with black skin stick is cylindrical, bacterium Bar gauge lattice are:Diameter 13-15cm, high 38-42cm, single bacteria stick weight 1.2-1.6kg.
3. the microwave sterilization method of claim 1-2 any one of them Termitomyces albuminosus with black skin bacteria sticks, which is characterized in that by casting skin chicken Fir bacterium bacteria stick, which is placed in microwave equipment cavity, carries out microwave sterilization processing, and sterilization process includes 2 stages of heat temperature raising and constant temperature; Wherein, the microwave power of heat temperature raising is 1.0-2.5kW, and the microwave heating heating-up time is 10-300min;The microwave power of constant temperature For 0-0.5kW, constant temperature time 0-300min.
4. the microwave sterilization method of Termitomyces albuminosus with black skin bacteria stick according to claim 3, which is characterized in that the heat temperature raising It is continuous with 2 stages of constant temperature.
5. the microwave sterilization method of Termitomyces albuminosus with black skin bacteria stick according to claim 3, which is characterized in that the constant temperature time by Microwave run time and heating dwell time composition, each microwave run time be 20 or 60s, heating dwell time be 40 or 60s。
6. the microwave sterilization method of Termitomyces albuminosus with black skin bacteria stick according to claim 5, which is characterized in that the constant temperature total time For 0-200min.
7. the microwave sterilization method of Termitomyces albuminosus with black skin bacteria stick according to claim 3, which is characterized in that the Termitomyces albuminosus with black skin Stick presses 34.5-181.5kg/m3Useful load be placed in microwave equipment cavity.
CN201810278941.XA 2018-03-30 2018-03-30 A kind of Termitomyces albuminosus with black skin bacteria stick and its microwave sterilization method Pending CN108307932A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101218876A (en) * 2007-12-26 2008-07-16 孙思国 Termitomyces albuminosus with black skin producing process
CN101935256A (en) * 2010-09-28 2011-01-05 上海市农业科学院 Pleurotus eryngii culture medium
CN104285663A (en) * 2013-07-16 2015-01-21 曲淑敏 Black termitomyces cultivation method
CN106613323A (en) * 2016-11-16 2017-05-10 贺州学院 Sterilization method of edible fungus stick
CN106718046A (en) * 2016-12-06 2017-05-31 贺州学院 A kind of simple sterilizing methods of edible fungus stick
CN107318466A (en) * 2017-07-28 2017-11-07 昆明海冠食用菌开发有限公司 A kind of method that highlands anti-season cultivates casting skin chicken fir

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101218876A (en) * 2007-12-26 2008-07-16 孙思国 Termitomyces albuminosus with black skin producing process
CN101935256A (en) * 2010-09-28 2011-01-05 上海市农业科学院 Pleurotus eryngii culture medium
CN104285663A (en) * 2013-07-16 2015-01-21 曲淑敏 Black termitomyces cultivation method
CN106613323A (en) * 2016-11-16 2017-05-10 贺州学院 Sterilization method of edible fungus stick
CN106718046A (en) * 2016-12-06 2017-05-31 贺州学院 A kind of simple sterilizing methods of edible fungus stick
CN107318466A (en) * 2017-07-28 2017-11-07 昆明海冠食用菌开发有限公司 A kind of method that highlands anti-season cultivates casting skin chicken fir

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