CN108293623A - A kind of lawn type of seeding - Google Patents
A kind of lawn type of seeding Download PDFInfo
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- CN108293623A CN108293623A CN201810047810.0A CN201810047810A CN108293623A CN 108293623 A CN108293623 A CN 108293623A CN 201810047810 A CN201810047810 A CN 201810047810A CN 108293623 A CN108293623 A CN 108293623A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention discloses a kind of lawn types of seeding, and steps are as follows：(1) it is 1 4cm straw clasts to smash straw waste at granularity, then by straw clast, soil, microbial-bacterial fertilizer and water (7 9) in mass ratio:(2‑4)：(1‑2)：(1 2) it is uniformly mixed, obtains lawn and cultivate soil；(2) lawn cultivation soil is evenly laid out on the soil for needing turf plant, it is 3 5cm then to cultivate soil to be densified to its thickness to lawn, obtains mixing careless base；(3) by grass-seed and fine earth or sandy soil in mass ratio 1：(5 10) it is uniformly mixed, then hole pressing is sown on the careless base of mixing, completes sowing.The present invention cultivates the major ingredient of soil using straw waste as lawn, using the degradation of microorganism, converts straw waste to fertilizer and used for lawn growth, meet demand of the lawn cultivating process to fertilizer, the dosage for reducing fertilizer reduces lawn and cultivates cost.
Description
Technical field
The invention belongs to lawns to cultivate planting technology field, and in particular to a kind of lawn type of seeding.
Background technology
In the ecosystem that the mankind are inhabited, lawn plays increasingly important role, it can not only beautify life
Environment purifies air, improves miniclimate, reduces noise, and in the ecological balance and the side of preventing erosion for safeguarding the Nature
Face plays an important role.As I accelerates with urbanization process, urban afforestation standard upgrading and urban size increase substantially,
The great demand to lawn is formd, market prospects are good.
The common lawn laying method in China various regions substantially has at present：The direct laying method of grass block；Lawn whitewashing laying method；Grass
Level ground tape planting laying method.(1) this method of the direct laying method of grass block is a kind of most common cover plant lawn method in China north and south various regions.I.e.
The excellent healthy and strong lawn by first grown, shovels it using grafter and removes, according to certain specification size, be laid with being carried to second
On the place on lawn, cover plant again is allowed to quickly form new meadow.(2) whitewashing laying method in lawn is to be equipped with air compression
Whitewashing unit grass-seed, fertilizer, moisturizer, herbicide, pigment and suitable pine can be blended with by stronger pressure
The green slurry liquid that soft organic matter and water etc. are configured to, Direct Uniform on spray feed to the level land or abrupt slope flattened.Its
In, the direct laying method of grass block is there is original arable land topsoil structure is destroyed, and lawn weight is heavy, it is inconvenient, of high cost etc. to transport
Problem.Lawn whitewashing laying method educates careless program complexity, and grass-seed after planting, in order to cover grass-seed, needs covering to be sieved above
Soil, laborious and human cost is high, and grass-seed cannot fully be combined with soil, and when glue is easy to run off.No matter using which kind of grass
Level ground laying method in the cultivating process of lawn, has that water, fertilizer demand are big, and uses general soil turf plant
When drainage problem be difficult to solve, influence lawn growth and effect.
Invention content
The object of the present invention is to provide a kind of lawn types of seeding.The lawn type of seeding using straw waste as
The major ingredient of soil is cultivated on lawn, is converted straw waste using the degradation of microorganism by adding microbial-bacterial fertilizer
It is used for lawn growth for fertilizer, meets the demand to fertilizer in the cultivating process of lawn, saved fertile in the cultivating process of lawn
The dosage of material, reduces the cultivation cost on lawn, moreover, turn waste into wealth, economical environment-protective.
To realize that goal of the invention, the technical solution adopted by the present invention are as follows：
The present invention provides a kind of lawn types of seeding, include the following steps：
(1) straw waste is smashed into the straw clast that granularity is 1-4cm, it is then that straw is broken
Bits, soil, microbial-bacterial fertilizer and water (7-9) in mass ratio:(2-4)：(1-2)：The ratio of (1-2) is uniformly mixed, and obtains lawn
Cultivate soil；
(2) lawn cultivation soil is evenly laid out on the soil for needing turf plant, the thickness that soil is cultivated on the lawn is
Then 8-10cm cultivates soil to lawn and carries out compaction treatment, until its thickness is 3-5cm, obtains mixing careless base；
(3) by grass-seed and fine earth or sandy soil in mass ratio 1：(5-10) is uniformly mixed, and then hole pressing is sown in the careless base of mixing
On, complete sowing.
According to above-mentioned lawn type of seeding, it is preferable that the microbial-bacterial fertilizer is by fertilizer and complex micro organism fungicide group
At addition 20-30ml complex micro organism fungicides in every 1kg fertilizer.It is highly preferred that the fertilizer is ammonium phosphate, plant ash, humic
Acid in mass ratio 6：4：3 mixtures mixed.The preparation method of the microbial-bacterial fertilizer is：By ammonium phosphate, grass in fertilizer
Wood ash, humic acid mass ratio weigh ammonium phosphate, plant ash and humic acid, be mixed evenly, obtain fertilizer；Then to fertilizer
Middle sprinkling complex micro organism fungicide, obtains microbial-bacterial fertilizer after mixing.
According to above-mentioned lawn type of seeding, it is preferable that the complex micro organism fungicide is by Phanerochaete chrysosporium
(Phanerochaete chrysosporium Burdsall) bacterium solution, bacillus subtilis (Bacillus subtilis) bacterium
Liquid, aspergillus niger (Aspergillus niger) bacterium solution and Trichoderma (Trichoderma spp.) bacterium solution by volume 5:3:4:2
It mixes, viable count is (3.5-4.0) × 10 in the Phanerochaete chrysosporium bacterium solution9A/ml, the bacillus subtilis
Viable count is (1.0-1.5) × 10 in bacterium solution10A/ml, viable count is (2.0-2.5) × 10 in the aspergillus niger bacterium solution9A/
Ml, viable count is (3.0-3.5) × 10 in the Trichoderma bacterium solution9A/ml.
According to above-mentioned lawn type of seeding, it is preferable that the preparation method of the complex micro organism fungicide is：
A. the preparation of Phanerochaete chrysosporium bacterium solution：Aseptically, the original strain of Phanerochaete chrysosporium is connect
Kind cultivates 2 days at 30 DEG C on PDA solid mediums, obtains slant strains；Slant strains are inoculated in PDB culture mediums,
120rpm cultivates 48h under the conditions of 30 DEG C, obtains seed liquor；By seed liquor by volume 1:15 are inoculated in fermentation tank (culture medium
For PDB culture mediums), 30 DEG C of fermented and cultured 72h obtain Phanerochaete chrysosporium bacterium solution；
B. the preparation of bacillus subtilis bacterium solution：Aseptically, the original strain of bacillus subtilis is inoculated in
On LB solid mediums, at 37 DEG C, culture for 24 hours, obtains slant strains；Slant strains are inoculated in LB liquid medium, 37
120rpm cultures for 24 hours, obtain primary seed solution under the conditions of DEG C；Then by primary seed solution by volume 1:15 are inoculated in fermentation tank
In (culture medium is LB liquid medium), 37 DEG C of fermented and cultured 60h obtain bacillus subtilis bacterium solution；
C. the preparation of aspergillus niger bacterium solution：Aseptically, the original strain of aspergillus niger is inoculated in PDA solid mediums
On, it is cultivated 2 days at 28 DEG C, obtains slant strains；Slant strains are inoculated in PDB culture mediums, 120rpm under the conditions of 28 DEG C
48h is cultivated, seed liquor is obtained；By seed liquor by volume 1:18 are inoculated in fermentation tank (culture medium is PDB culture mediums), 28 DEG C
Fermented and cultured 72h obtains aspergillus niger bacterium solution；
D. the preparation of Trichoderma bacterium solution：Aseptically, the original strain of Trichoderma is inoculated in PDA solid mediums
On, it is cultivated 2 days at 28 DEG C, obtains slant strains；Slant strains are inoculated in PDB culture mediums, 120rpm under the conditions of 28 DEG C
36h is cultivated, seed liquor is obtained；By seed liquor by volume 1:20 are inoculated in fermentation tank (culture medium is PDB culture mediums), 28 DEG C
Fermented and cultured 72h obtains Trichoderma bacterium solution；
E. by volume by Phanerochaete chrysosporium bacterium solution, bacillus subtilis bacterium solution, aspergillus niger bacterium solution and Trichoderma liquor
5:3:4:2 are uniformly mixed, and obtain complex micro organism fungicide.
The positive beneficial effect that the present invention obtains：
(1) present invention cultivates the major ingredient of soil using straw waste as lawn, by adding microbial-bacterial fertilizer, utilizes
The degradation of microorganism converts straw waste to fertilizer and is used for lawn growth, meets lawn cultivating process
In demand to fertilizer, saved the dosage of fertilizer in the cultivating process of lawn, reduced the cultivation cost on lawn, moreover, becoming useless
For treasured, economical environment-protective.
(2) present invention cultivates the major ingredient of soil, the mixing grass datum hole being prepared using straw waste as lawn
Porosity is big, and water penetration is strong, solves the problems, such as to drain hardly possible using existing for normal soil cultivation lawn.
(3) present invention sows grass-seed by the way of bunch planting on careless base, and grass-seed can come into full contact with careless base, improve
Grass-seed emergence rate and survival rate, moreover, lawn long lifespan fast at level ground.
(4) complex micro organism fungicide, composite microbial bacteria has been made using the conspiracy relation between a variety of bacterial strains in the present invention
Phanerochaete chrysosporium in agent has good degradation capability to lignin, can make the lignin degradation being wrapped in outside cellulose,
Be conducive to that cellulose is exposed, to enhance degradation efficiency of other microorganisms to cellulose；Trichoderma and aspergillus niger cellulase
Activity is very high, can effective degraded cellulose and hemicellulose, bacillus subtilis can degrade the macromoleculars such as carbohydrate, starch
Organic substance, and there is certain resistant effect.Therefore, the microorganism in complex micro organism fungicide can give up straw
Gurry Degradation and Transformation provides fertilizer for lawn growth, meets lawn growth use, also has and inhibits and kill the micro- life of pathogenic soil
The effect of object.
Specific implementation mode
Below by way of specific embodiment, the present invention will be further described in detail, but is not intended to limit the model of the present invention
It encloses.Without departing from the spirit and substance of the case in the present invention, to modifications or substitutions made by the method for the present invention, step or condition,
It all belongs to the scope of the present invention.
Unless otherwise specified, the conventional means that technological means used in embodiment is well known to those skilled in the art,
Reagent used is also this field conventional reagent.
Embodiment 1：The preparation of complex micro organism fungicide
(1) preparation of Phanerochaete chrysosporium bacterium solution：Aseptically, the original strain of Phanerochaete chrysosporium is connect
Kind cultivates 2 days at 30 DEG C on PDA solid mediums, obtains slant strains；Slant strains are inoculated in PDB culture mediums,
120rpm cultivates 48h under the conditions of 30 DEG C, obtains seed liquor；By seed liquor by volume 1:15 are inoculated in fermentation tank (culture medium
For PDB culture mediums), fermented and cultured 72h under conditions of low whipping speed 150rpm, ventilatory capacity 6slpm, temperature are 30 DEG C,
Phanerochaete chrysosporium bacterium solution is obtained, viable count is (3.5-4.0) × 10 in the Phanerochaete chrysosporium bacterium solution9A/ml；
(2) preparation of bacillus subtilis bacterium solution：Aseptically, the original strain of bacillus subtilis is inoculated in
On LB solid mediums, at 37 DEG C, culture for 24 hours, obtains slant strains；Slant strains are inoculated in LB liquid medium, 37
120rpm cultures for 24 hours, obtain primary seed solution under the conditions of DEG C；Then by primary seed solution by volume 1:15 are inoculated in fermentation tank
In (culture medium is LB liquid medium), under conditions of low whipping speed 150rpm, ventilatory capacity 6slpm, temperature are 37 DEG C
Fermented and cultured 60h obtains bacillus subtilis bacterium solution, and viable count is (1.0-1.5) × 10 in the bacillus subtilis bacterium solution10
A/ml；
(3) preparation of aspergillus niger bacterium solution：Aseptically, the original strain of aspergillus niger is inoculated in PDA solid cultures
On base, is cultivated 2 days at 28 DEG C, obtain slant strains；Slant strains are inoculated in PDB culture mediums, under the conditions of 28 DEG C
120rpm cultivates 48h, obtains seed liquor；By seed liquor by volume 1:18 it is inoculated in fermentation tank that (culture medium is PDB cultures
Base), fermented and cultured 72h under conditions of low whipping speed 150rpm, ventilatory capacity 6slpm, temperature are 28 DEG C obtains aspergillus niger
Bacterium solution, viable count is (2.0-2.5) × 10 in the aspergillus niger bacterium solution9A/ml；
(4) preparation of Trichoderma liquor：Aseptically, the original strain of trichoderma is inoculated on PDA solid mediums,
It is cultivated 2 days at 28 DEG C, obtains slant strains；Slant strains are inoculated in PDB culture mediums, 120rpm is cultivated under the conditions of 28 DEG C
36h obtains seed liquor；By seed liquor by volume 1:20 are inoculated in fermentation tank (culture medium is PDB culture mediums), in stirring speed
Fermented and cultured 72h under conditions of degree is 150rpm, ventilatory capacity 6slpm, temperature are 28 DEG C, obtains Trichoderma liquor, the trichoderma
Viable count is (3.0-3.5) × 10 in bacterium bacterium solution9A/ml；
(5) by volume by Phanerochaete chrysosporium bacterium solution, bacillus subtilis bacterium solution, aspergillus niger bacterium solution and Trichoderma liquor
5:3:4:2 are uniformly mixed, and obtain complex micro organism fungicide.
Embodiment 2：
A kind of lawn type of seeding, includes the following steps：
(1) straw waste is smashed into the straw clast that granularity is 1-4cm, it is then that straw is broken
Bits, soil, microbial-bacterial fertilizer and water in mass ratio 7：2：1：1 ratio is uniformly mixed, and is obtained lawn and is cultivated soil；
(2) lawn cultivation soil is evenly laid out on the soil for needing turf plant, the thickness that soil is cultivated on the lawn is
Then 10cm cultivates soil to lawn and carries out compaction treatment, until its thickness is 5cm, obtains mixing careless base；
(3) by grass-seed and fine earth in mass ratio 1：5 are uniformly mixed, and then hole pressing is sown on the careless base of mixing, completes sowing.
Wherein, the microbial-bacterial fertilizer is made of fertilizer and complex micro organism fungicide, and addition 20ml is compound in every 1kg fertilizer
Microbial bacterial agent；The fertilizer is ammonium phosphate, plant ash, humic acid in mass ratio 6：4：3 mixtures mixed；It is described multiple
It is by Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall) bacterium solution, withered grass to close microbial bacterial agent
Bacillus (Bacillus subtilis) bacterium solution, aspergillus niger (Aspergillus niger) bacterium solution and Trichoderma
(Trichoderma spp.) bacterium solution by volume 5:3:4:2 mix, viable count in the Phanerochaete chrysosporium bacterium solution
For (3.5-4.0) × 109A/ml, viable count is (1.0-1.5) × 10 in the bacillus subtilis bacterium solution10A/ml, it is described
Viable count is (2.0-2.5) × 10 in aspergillus niger bacterium solution9A/ml, viable count is (3.0-3.5) × 10 in the Trichoderma bacterium solution9
A/ml.
The preparation method of the microbial-bacterial fertilizer is：Phosphorus is weighed by the mass ratio of ammonium phosphate in fertilizer, plant ash, humic acid
Sour ammonium, plant ash and humic acid, are mixed evenly, and obtain fertilizer；Then complex micro organism fungicide is sprayed into fertilizer, is mixed
Microbial-bacterial fertilizer is obtained after uniformly.
Embodiment 3：
A kind of lawn type of seeding, includes the following steps：
(1) straw waste is smashed into the straw clast that granularity is 1-4cm, it is then that straw is broken
Bits, soil, microbial-bacterial fertilizer and water in mass ratio 9：4：2：2 ratio is uniformly mixed, and is obtained lawn and is cultivated soil；
(2) lawn cultivation soil is evenly laid out on the soil for needing turf plant, the thickness that soil is cultivated on the lawn is
Then 8cm cultivates soil to lawn and carries out compaction treatment, until its thickness is 3cm, obtains mixing careless base；
(3) by grass-seed and sandy soil in mass ratio 1：10 are uniformly mixed, and then hole pressing is sown on the careless base of mixing, completes to broadcast
Kind.
Wherein, the microbial-bacterial fertilizer is made of fertilizer and complex micro organism fungicide, and addition 30ml is compound in every 1kg fertilizer
Microbial bacterial agent；The fertilizer is ammonium phosphate, plant ash, humic acid in mass ratio 6：4：3 mixtures mixed；It is described multiple
It is by Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall) bacterium solution, withered grass to close microbial bacterial agent
Bacillus (Bacillus subtilis) bacterium solution, aspergillus niger (Aspergillus niger) bacterium solution and Trichoderma
(Trichoderma spp.) bacterium solution by volume 5:3:4:2 mix, viable count in the Phanerochaete chrysosporium bacterium solution
For (3.5-4.0) × 109A/ml, viable count is (1.0-1.5) × 10 in the bacillus subtilis bacterium solution10A/ml, it is described
Viable count is (2.0-2.5) × 10 in aspergillus niger bacterium solution9A/ml, viable count is (3.0-3.5) × 10 in the Trichoderma bacterium solution9
A/ml.
The preparation method of the microbial-bacterial fertilizer is：Phosphorus is weighed by the mass ratio of ammonium phosphate in fertilizer, plant ash, humic acid
Sour ammonium, plant ash and humic acid, are mixed evenly, and obtain fertilizer；Then complex micro organism fungicide is sprayed into fertilizer, is mixed
Microbial-bacterial fertilizer is obtained after uniformly.
Embodiment 4：
A kind of lawn type of seeding, includes the following steps：
(1) straw waste is smashed into the straw clast that granularity is 1-4cm, it is then that straw is broken
Bits, soil, microbial-bacterial fertilizer and water in mass ratio 8：3：2：1 ratio is uniformly mixed, and is obtained lawn and is cultivated soil；
(2) lawn cultivation soil is evenly laid out on the soil for needing turf plant, the thickness that soil is cultivated on the lawn is
Then 9cm cultivates soil to lawn and carries out compaction treatment, until its thickness is 4cm, obtains mixing careless base；
(3) by grass-seed and sandy soil in mass ratio 1：8 are uniformly mixed, and then hole pressing is sown on the careless base of mixing, completes sowing.
Wherein, the microbial-bacterial fertilizer is made of fertilizer and complex micro organism fungicide, and addition 25ml is compound in every 1kg fertilizer
Microbial bacterial agent；The fertilizer is ammonium phosphate, plant ash, humic acid in mass ratio 6：4：3 mixtures mixed；It is described multiple
It is by Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall) bacterium solution, withered grass to close microbial bacterial agent
Bacillus (Bacillus subtilis) bacterium solution, aspergillus niger (Aspergillus niger) bacterium solution and Trichoderma
(Trichoderma spp.) bacterium solution by volume 5:3:4:2 mix, viable count in the Phanerochaete chrysosporium bacterium solution
For (3.5-4.0) × 109A/ml, viable count is (1.0-1.5) × 10 in the bacillus subtilis bacterium solution10A/ml, it is described
Viable count is (2.0-2.5) × 10 in aspergillus niger bacterium solution9A/ml, viable count is (3.0-3.5) × 10 in the Trichoderma bacterium solution9
A/ml.
The preparation method of the microbial-bacterial fertilizer is：Phosphorus is weighed by the mass ratio of ammonium phosphate in fertilizer, plant ash, humic acid
Sour ammonium, plant ash and humic acid, are mixed evenly, and obtain fertilizer；Then complex micro organism fungicide is sprayed into fertilizer, is mixed
Microbial-bacterial fertilizer is obtained after uniformly.
Embodiment 5：
A kind of lawn type of seeding, includes the following steps：
(1) straw waste is smashed into the straw clast that granularity is 1-4cm, it is then that straw is broken
Bits, soil, microbial-bacterial fertilizer and water in mass ratio 8：4：2：1 ratio is uniformly mixed, and is obtained lawn and is cultivated soil；
(2) lawn cultivation soil is evenly laid out on the soil for needing turf plant, the thickness that soil is cultivated on the lawn is
Then 10cm cultivates soil to lawn and carries out compaction treatment, until its thickness is 4cm, obtains mixing careless base；
(3) by grass-seed and fine earth in mass ratio 1：6 are uniformly mixed, and then hole pressing is sown on the careless base of mixing, completes sowing.
Wherein, the microbial-bacterial fertilizer is made of fertilizer and complex micro organism fungicide, and addition 25ml is compound in every 1kg fertilizer
Microbial bacterial agent；The fertilizer is ammonium phosphate, plant ash, humic acid in mass ratio 6：4：3 mixtures mixed；It is described multiple
It is by Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall) bacterium solution, withered grass to close microbial bacterial agent
Bacillus (Bacillus subtilis) bacterium solution, aspergillus niger (Aspergillus niger) bacterium solution and Trichoderma
(Trichoderma spp.) bacterium solution by volume 5:3:4:2 mix, viable count in the Phanerochaete chrysosporium bacterium solution
For (3.5-4.0) × 109A/ml, viable count is (1.0-1.5) × 10 in the bacillus subtilis bacterium solution10A/ml, it is described
Viable count is (2.0-2.5) × 10 in aspergillus niger bacterium solution9A/ml, viable count is (3.0-3.5) × 10 in the Trichoderma bacterium solution9
A/ml.
The preparation method of the microbial-bacterial fertilizer is：Phosphorus is weighed by the mass ratio of ammonium phosphate in fertilizer, plant ash, humic acid
Sour ammonium, plant ash and humic acid, are mixed evenly, and obtain fertilizer；Then complex micro organism fungicide is sprayed into fertilizer, is mixed
Microbial-bacterial fertilizer is obtained after uniformly.
Claims (6)
1. a kind of lawn type of seeding, which is characterized in that include the following steps：
(1) straw waste is smashed at the straw clast that granularity is 1-4cm, then by straw clast, soil
Earth, microbial-bacterial fertilizer and water (7-9) in mass ratio:(2-4)：(1-2)：The ratio of (1-2) is uniformly mixed, and obtains lawn cultivation
Soil；
(2) lawn cultivation soil is evenly laid out on the soil for needing turf plant, the thickness that soil is cultivated on the lawn is 8-
Then 10cm cultivates soil to lawn and carries out compaction treatment, until its thickness is 3-5cm, obtains mixing careless base；
(3) by grass-seed and fine earth or sandy soil in mass ratio 1：(5-10) is uniformly mixed, and then hole pressing is sown on the careless base of mixing, complete
At sowing.
2. lawn type of seeding according to claim 1, which is characterized in that the microbial-bacterial fertilizer is by fertilizer and compound micro-
Bacteria agent forms, and 20-30ml complex micro organism fungicides are added in every 1kg fertilizer.
3. lawn type of seeding according to claim 2, which is characterized in that the fertilizer is ammonium phosphate, plant ash, humic
Acid in mass ratio 6：4：3 mixtures mixed.
4. lawn type of seeding according to claim 3, which is characterized in that the preparation method of the microbial-bacterial fertilizer is：
Ammonium phosphate, plant ash and humic acid are weighed by the mass ratio of ammonium phosphate in fertilizer, plant ash, humic acid, is mixed evenly, obtains
To fertilizer；Then complex micro organism fungicide is sprayed into fertilizer, obtains microbial-bacterial fertilizer after mixing.
5. lawn type of seeding according to claim 2, which is characterized in that the complex micro organism fungicide is by yellow archespore
Flat lead fungi (the Phanerochaete chrysosporium Burdsall) bacterium solution of hair, bacillus subtilis (Bacillus
Subtilis) bacterium solution, aspergillus niger (Aspergillus niger) bacterium solution and Trichoderma (Trichoderma spp.) bacterium solution press body
Product ratio 5:3:4:2 mix, and viable count is (3.5-4.0) × 10 in the Phanerochaete chrysosporium bacterium solution9A/ml, it is described
Viable count is (1.0-1.5) × 10 in bacillus subtilis bacterium solution10A/ml, viable count is (2.0- in the aspergillus niger bacterium solution
2.5)×109A/ml, viable count is (3.0-3.5) × 10 in the Trichoderma bacterium solution9A/ml.
6. lawn type of seeding according to claim 5, which is characterized in that the preparation method of the complex micro organism fungicide
For：
A. the preparation of Phanerochaete chrysosporium bacterium solution：Aseptically, the original strain of Phanerochaete chrysosporium is inoculated in
On PDA solid mediums, is cultivated 2 days at 30 DEG C, obtain slant strains；Slant strains are inoculated in PDB culture mediums, at 30 DEG C
Under the conditions of 120rpm cultivate 48h, obtain seed liquor；By seed liquor by volume 1:15 are inoculated in fermentation tank (culture medium PDB
Culture medium), 30 DEG C of fermented and cultured 72h obtain Phanerochaete chrysosporium bacterium solution；
B. the preparation of bacillus subtilis bacterium solution：Aseptically, the original strain of bacillus subtilis LB is inoculated in consolidate
On body culture medium, at 37 DEG C, culture for 24 hours, obtains slant strains；Slant strains are inoculated in LB liquid medium, in 37 DEG C of items
120rpm cultures for 24 hours, obtain primary seed solution under part；Then by primary seed solution by volume 1:15 are inoculated in fermentation tank
(culture medium is LB liquid medium), 37 DEG C of fermented and cultured 60h, obtains bacillus subtilis bacterium solution；
C. the preparation of aspergillus niger bacterium solution：Aseptically, the original strain of aspergillus niger is inoculated on PDA solid mediums,
It is cultivated 2 days at 28 DEG C, obtains slant strains；Slant strains are inoculated in PDB culture mediums, 120rpm is cultivated under the conditions of 28 DEG C
48h obtains seed liquor；By seed liquor by volume 1:18 are inoculated in fermentation tank (culture medium is PDB culture mediums), 28 DEG C of fermentations
72h is cultivated, aspergillus niger bacterium solution is obtained；
D. the preparation of Trichoderma bacterium solution：Aseptically, the original strain of Trichoderma is inoculated on PDA solid mediums,
It is cultivated 2 days at 28 DEG C, obtains slant strains；Slant strains are inoculated in PDB culture mediums, 120rpm is cultivated under the conditions of 28 DEG C
36h obtains seed liquor；By seed liquor by volume 1:20 are inoculated in fermentation tank (culture medium is PDB culture mediums), 28 DEG C of fermentations
72h is cultivated, Trichoderma bacterium solution is obtained；
E. by Phanerochaete chrysosporium bacterium solution, bacillus subtilis bacterium solution, aspergillus niger bacterium solution and Trichoderma bacterium solution by volume 5:
3:4:2 are uniformly mixed, and obtain complex micro organism fungicide.
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| CN201810047810.0A CN108293623A (en) | 2018-01-18 | 2018-01-18 | A kind of lawn type of seeding |
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| CN201810047810.0A CN108293623A (en) | 2018-01-18 | 2018-01-18 | A kind of lawn type of seeding |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110226489A (en) * | 2019-06-27 | 2019-09-13 | 深圳市铁汉生态环境股份有限公司 | A kind of slope greening is sowed grass seeds by duster fiber matrix material and preparation method thereof and application method |
| CN113045364A (en) * | 2021-03-19 | 2021-06-29 | 广东省林业科学研究院 | Garden waste pretreatment and composting method |
Citations (7)
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| CN101844367A (en) * | 2010-05-26 | 2010-09-29 | 西安科技大学 | Method for manufacturing meadow tiles by crop straws |
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| CN110226489A (en) * | 2019-06-27 | 2019-09-13 | 深圳市铁汉生态环境股份有限公司 | A kind of slope greening is sowed grass seeds by duster fiber matrix material and preparation method thereof and application method |
| CN110226489B (en) * | 2019-06-27 | 2022-05-03 | 深圳市铁汉生态环境股份有限公司 | Slope greening spray-seeding fiber matrix material and preparation method and use method thereof |
| CN113045364A (en) * | 2021-03-19 | 2021-06-29 | 广东省林业科学研究院 | Garden waste pretreatment and composting method |
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