CN108244328A - A kind of processing method of method of rice protein hydrolysate powder - Google Patents
A kind of processing method of method of rice protein hydrolysate powder Download PDFInfo
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- CN108244328A CN108244328A CN201810107398.7A CN201810107398A CN108244328A CN 108244328 A CN108244328 A CN 108244328A CN 201810107398 A CN201810107398 A CN 201810107398A CN 108244328 A CN108244328 A CN 108244328A
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- rice
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- protein hydrolysate
- hydrolysate powder
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/12—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses
- A23J1/125—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses by treatment involving enzymes or microorganisms
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- Chemical & Material Sciences (AREA)
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- Cereal-Derived Products (AREA)
Abstract
The invention discloses a kind of processing methods of method of rice protein hydrolysate powder, the present invention first cleans rice before the extraction of method of rice protein hydrolysate powder, drying, freezing processing, it can understand rice surface impurity, the nutriment of rice can be retained again, the present invention first cleans rice before the extraction of method of rice protein hydrolysate powder, freezing, it impregnates, infusion, grinding process, rice, which is cleaned, with cleaning solution can remove rice surface impurity, remove pesticide residue, emollescence can be played to rice again, the nutriment of rice can be retained again, be conducive to the extraction of follow-up method of rice protein hydrolysate powder.
Description
Technical field：
The present invention relates to rice processing technique fields, and in particular to a kind of processing method of method of rice protein hydrolysate powder.
Background technology：
Rice is paddy manufactured finished product after the processes such as cleaning, husking, husk rice, finished product arrangement.Rice is the master of the mankind
One of food, is analyzed according to modern nutriology, and rice contains protein, fat, vitamin B1, A, E and several mineral materials.With regard to kind
Speech, rice are divided into stalk rice and glutinous rice.Carbohydrate containing 75% or so in rice, protein 7%-8%, fatty 1.3%-
1.8%, and contain abundant B family vitamin etc..Carbohydrate in polished rice rice is mainly starch, contained protein master
If oryzenin, secondly oryzenin and globulin, the biological value of protein and the composition of amino acid are all than small
The cereal crops such as wheat, barley, millet, corn are high, higher by one in digestibility 66.8%-83.1% and grain protein
Kind.Therefore, edible rice has higher nutritive value.But the comparision contents of lysine and threonine are few in rice protein, institute
Not to be a kind of complete protein, nutritive value is less than animal protein.
Invention content：
The technical problems to be solved by the invention are to provide a kind of easy to operation, product recovery rate height and purity is high
Method of rice protein hydrolysate powder processing method.
The technical problems to be solved by the invention are realized using following technical solution：
A kind of processing method of method of rice protein hydrolysate powder, includes the following steps：
1) rice first with cleaning agent is impregnated 20-30 minutes, then is cleaned up with clear water, dried；
2) rice after drying is put into refrigerating chamber, is freeze-dried, freezer temperature is first cooled to -5~-10 DEG C,
Freezing 2 hours, then 0~5 DEG C is warming up to, it is kept for 20 minutes, then be warming up to 5~10 DEG C, is kept for 20 minutes；
3) rice after freezing is impregnated 80-100 minutes with 2 times of clear water；
4) rice of immersion together with soak is heated, after boiling 15-20 minutes, closes fire and simmer 20-30 minutes；
5) the well-done liquid of step 4 is subjected to defibrination with colloid mill, the polishing time is 5-10 minutes；
6) complex enzyme is added in the liquid that step 5 has been polished, and heat preservation enzymolysis is carried out at a temperature of 35-45 DEG C, then
Reflux state insulated and stirred 10-15min is heated to, centrifuges, takes upper strata enzymolysis liquid；
7) NF membrane using retention relative molecular mass 180-200Da is used to carry out nanofiltration desalination enzymolysis liquid；
8) enzymolysis liquid that step 7 is handled well is concentrated in vacuo, the concentrate of 30%-35% is obtained after concentration；
9) concentrate is sent into freeze drier, micro mist is made through micronizer in dry obtained solid, obtains rice
Protein hydrolysate powder.
The complex enzyme is by mass ratio 2：1：1 protease, amylase and cellulase composition.
Further, the cleaning agent is prepared from the following raw materials in parts by weight：8-12 parts of starch, 3-8 parts of salt, vinegar 2-4
Part, 1-3 parts of citric acid, 0.5-1 parts of pectin, microcrystalline cellulose -2-5 parts of glycine.
The specific preparation method of the microcrystalline cellulose-glycine is：It is warming up to after microcrystalline cellulose, glycine are mixed
80-85 DEG C heat preservation grinding 10min, then add in deionized water until mass water content reach 80-85%, be sufficiently stirred and utilize
Microwave treater microwave reflow treatment 5min, continues microwave reflow treatment 5min after being spaced 5min, is spaced microwave after 5min again
Reflow treatment 5min, repeats operation, and controlling total microwave reflow treatment, after treatment naturally cools to room in 25-35min
Temperature, gained mixture are sent into freeze drier, and micro mist is made to get microcrystalline cellulose through micronizer in dry obtained solid
Element-glycine.
The microcrystalline cellulose, glycine mass ratio in 1-5:5-10.
The beneficial effects of the invention are as follows：
(1) present invention first rice cleaned, freezed, impregnated before the extraction of method of rice protein hydrolysate powder, infusion, burnishing part
Reason, rice, which is cleaned, with cleaning solution can remove rice surface impurity, remove pesticide residue, and softening can be played to rice
Effect, and the nutriment of rice can be retained, be conducive to the extraction of follow-up method of rice protein hydrolysate powder；
(2) present invention extracts method of rice protein hydrolysate powder with conbined enzymolysis, is made with protease, amylase and cellulase
For complex enzyme, the leaching velocity of protein is dramatically speeded up, and extraction conditions are mild, energy consumption input cost is small, while avoids growing
Time high temperature, which extracts, destroys the structure of protein, so as to improve the recovery rate of protein；
(3) present invention isolates and purifies protein using macroporous absorbent resin mixed bed, in protein obtained by raising
The time required to purifying is greatly shortened while purity, the loss amount of the protein in purification process is reduced.
Specific embodiment：
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1
A kind of processing method of method of rice protein hydrolysate powder, includes the following steps：
1) rice first with cleaning agent is impregnated 20 minutes, then is cleaned up with clear water, dried；
2) rice after drying is put into refrigerating chamber, be freeze-dried, freezer temperature is first cooled to -5 DEG C, freezing 2
Hour, then 0 DEG C is warming up to, it is kept for 20 minutes, then be warming up to 5 DEG C, is kept for 20 minutes；
3) rice after freezing is impregnated 80 minutes with 2 times of clear water；
4) rice of immersion together with soak is heated, after boiling 15 minutes, closes fire and simmer 20 minutes；
5) the well-done liquid of step 4 is subjected to defibrination with colloid mill, the polishing time is 5 minutes；
6) complex enzyme is added in the liquid that step 5 has been polished, and heat preservation enzymolysis is carried out at a temperature of 35 DEG C, is reheated
To reflux state insulated and stirred 10min, centrifugation takes upper strata enzymolysis liquid；
7) NF membrane using retention relative molecular mass 180Da is used to carry out nanofiltration desalination enzymolysis liquid；
8) enzymolysis liquid that step 7 is handled well is concentrated in vacuo, 30% concentrate is obtained after concentration；
9) concentrate is sent into freeze drier, micro mist is made through micronizer in dry obtained solid, obtains rice
Protein hydrolysate powder.
The complex enzyme is by mass ratio 2：1：1 protease, amylase and cellulase composition.
Further, the cleaning agent is prepared from the following raw materials in parts by weight：8 parts of starch, 3 parts of salt, 2 parts of vinegar, lemon
1 part of acid, 0.5 part of pectin, 2 parts of microcrystalline cellulose-glycine.
The specific preparation method of the microcrystalline cellulose-glycine is：It is warming up to after microcrystalline cellulose, glycine are mixed
80 DEG C heat preservation grinding 10min, then add in deionized water until mass water content reach 80%, be sufficiently stirred and using microwave at
Device microwave reflow treatment 5min is managed, continues microwave reflow treatment 5min after being spaced 5min, again after the 5min of interval at microwave reflux
5min is managed, is repeated operation, controls total microwave reflow treatment in 25min, after treatment cooled to room temperature, gained mixes
It closes object to be sent into freeze drier, micro mist is made to get microcrystalline cellulose-glycine through micronizer in dry obtained solid.
The microcrystalline cellulose, glycine mass ratio 1:5.
Embodiment 2
A kind of processing method of method of rice protein hydrolysate powder, includes the following steps：
1) rice first with cleaning agent is impregnated 30 minutes, then is cleaned up with clear water, dried；
2) rice after drying is put into refrigerating chamber, be freeze-dried, freezer temperature is first cooled to -10 DEG C, freezing 2
Hour, then 5 DEG C are warming up to, it is kept for 20 minutes, then be warming up to 10 DEG C, is kept for 20 minutes；
3) rice after freezing is impregnated 100 minutes with 2 times of clear water；
4) rice of immersion together with soak is heated, boiled after twenty minutes, closed fire and simmer 30 minutes；
5) the well-done liquid of step 4 is subjected to defibrination with colloid mill, the polishing time is 10 minutes；
6) complex enzyme is added in the liquid that step 5 has been polished, and heat preservation enzymolysis is carried out at a temperature of 45 DEG C, is reheated
To reflux state insulated and stirred 15min, centrifugation takes upper strata enzymolysis liquid；
7) NF membrane using retention relative molecular mass 200Da is used to carry out nanofiltration desalination enzymolysis liquid；
8) enzymolysis liquid that step 7 is handled well is concentrated in vacuo, 35% concentrate is obtained after concentration；
9) concentrate is sent into freeze drier, micro mist is made through micronizer in dry obtained solid, obtains rice
Protein hydrolysate powder.
The complex enzyme is by mass ratio 2：1：1 protease, amylase and cellulase composition.
Further, the cleaning agent is prepared from the following raw materials in parts by weight：12 parts of starch, 8 parts of salt, 4 parts of vinegar, lemon
3 parts of acid, 1 part of pectin, 5 parts of microcrystalline cellulose-glycine.
The specific preparation method of the microcrystalline cellulose-glycine is：It is warming up to after microcrystalline cellulose, glycine are mixed
85 DEG C heat preservation grinding 10min, then add in deionized water until mass water content reach 85%, be sufficiently stirred and using microwave at
Device microwave reflow treatment 5min is managed, continues microwave reflow treatment 5min after being spaced 5min, again after the 5min of interval at microwave reflux
5min is managed, is repeated operation, controls total microwave reflow treatment in 35min, after treatment cooled to room temperature, gained mixes
It closes object to be sent into freeze drier, micro mist is made to get microcrystalline cellulose-glycine through micronizer in dry obtained solid.
The microcrystalline cellulose, glycine mass ratio 5:10.
Embodiment 3
A kind of processing method of method of rice protein hydrolysate powder, includes the following steps：
1) rice first with cleaning agent is impregnated 25 minutes, then is cleaned up with clear water, dried；
2) rice after drying is put into refrigerating chamber, be freeze-dried, freezer temperature is first cooled to -8 DEG C, freezing 2
Hour, then 3 DEG C are warming up to, it is kept for 20 minutes, then be warming up to 8 DEG C, is kept for 20 minutes；
3) rice after freezing is impregnated 90 minutes with 2 times of clear water；
4) rice of immersion together with soak is heated, after boiling 18 minutes, closes fire and simmer 25 minutes；
5) the well-done liquid of step 4 is subjected to defibrination with colloid mill, the polishing time is 8 minutes；
6) complex enzyme is added in the liquid that step 5 has been polished, and heat preservation enzymolysis is carried out at a temperature of 40 DEG C, is reheated
To reflux state insulated and stirred 14min, centrifugation takes upper strata enzymolysis liquid；
7) NF membrane using retention relative molecular mass 190Da is used to carry out nanofiltration desalination enzymolysis liquid；
8) enzymolysis liquid that step 7 is handled well is concentrated in vacuo, the concentrate of 30%-35% is obtained after concentration；
9) concentrate is sent into freeze drier, micro mist is made through micronizer in dry obtained solid, obtains rice
Protein hydrolysate powder.
The complex enzyme is by mass ratio 2：1：1 protease, amylase and cellulase composition.
Further, the cleaning agent is prepared from the following raw materials in parts by weight：10 parts of starch, 6 parts of salt, 3 parts of vinegar, lemon
2 parts of acid, 0.8 part of pectin, 4 parts of microcrystalline cellulose-glycine.
The specific preparation method of the microcrystalline cellulose-glycine is：It is warming up to after microcrystalline cellulose, glycine are mixed
83 DEG C heat preservation grinding 10min, then add in deionized water until mass water content reach 84%, be sufficiently stirred and using microwave at
Device microwave reflow treatment 5min is managed, continues microwave reflow treatment 5min after being spaced 5min, again after the 5min of interval at microwave reflux
5min is managed, is repeated operation, controls total microwave reflow treatment in 30min, after treatment cooled to room temperature, gained mixes
It closes object to be sent into freeze drier, micro mist is made to get microcrystalline cellulose-glycine through micronizer in dry obtained solid.
The microcrystalline cellulose, glycine mass ratio 3:8.
The extraction result of protein in 1 rice of the present invention of table
| Group | Recovery rate mg/100g | Purity % |
| Embodiment 1 | 285.86 | 89.25 |
| Embodiment 2 | 289.34 | 92.03 |
| Embodiment 3 | 287.51 | 91.52 |
The basic principles, main features and the advantages of the invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (3)
1. a kind of processing method of method of rice protein hydrolysate powder, which is characterized in that include the following steps：
1) rice first with cleaning agent is impregnated 20-30 minutes, then is cleaned up with clear water, dried；
2) rice after drying is put into refrigerating chamber, be freeze-dried, freezer temperature is first cooled to -5~-10 DEG C, freezing 2
Hour, then 0~5 DEG C is warming up to, it is kept for 20 minutes, then be warming up to 5~10 DEG C, is kept for 20 minutes；
3) rice after freezing is impregnated 80-100 minutes with 2 times of clear water；
4) rice of immersion together with soak is heated, after boiling 15-20 minutes, closes fire and simmer 20-30 minutes；
5) the well-done liquid of step 4 is subjected to defibrination with colloid mill, the polishing time is 5-10 minutes；
6) complex enzyme is added in the liquid that step 5 has been polished, and heat preservation enzymolysis is carried out at a temperature of 35-45 DEG C, is reheated
To reflux state insulated and stirred 10-15min, centrifugation takes upper strata enzymolysis liquid；
7) NF membrane using retention relative molecular mass 180-200Da is used to carry out nanofiltration desalination enzymolysis liquid；
8) enzymolysis liquid that step 7 is handled well is concentrated in vacuo, the concentrate of 30%-35% is obtained after concentration；
9) concentrate is sent into freeze drier, micro mist is made through micronizer in dry obtained solid, obtains rice hydrolysis
Albumen powder.
2. the processing method of method of rice protein hydrolysate powder according to claim 1, it is characterised in that：The complex enzyme is by matter
Measure ratio 2：1：1 protease, amylase and cellulase composition.
3. the processing method of method of rice protein hydrolysate powder according to claim 1, it is characterised in that：Further, it is described clear
Lotion is prepared from the following raw materials in parts by weight：8-12 parts of starch, 3-8 parts of salt, 2-4 parts of vinegar, 1-3 parts of citric acid, pectin 0.5-1
Part, microcrystalline cellulose -2-5 parts of glycine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810107398.7A CN108244328A (en) | 2018-02-02 | 2018-02-02 | A kind of processing method of method of rice protein hydrolysate powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810107398.7A CN108244328A (en) | 2018-02-02 | 2018-02-02 | A kind of processing method of method of rice protein hydrolysate powder |
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| CN108244328A true CN108244328A (en) | 2018-07-06 |
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| CN201810107398.7A Pending CN108244328A (en) | 2018-02-02 | 2018-02-02 | A kind of processing method of method of rice protein hydrolysate powder |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109170561A (en) * | 2018-09-11 | 2019-01-11 | 西安禾其果商贸股份有限公司 | Selenium-rich rice extract and the preparation method and application thereof |
| CN110089616A (en) * | 2019-05-14 | 2019-08-06 | 江西虔农农业股份有限公司 | A kind of processing method of selenium-rich hydrolysis rice protein cream and powder |
| WO2020113975A1 (en) * | 2018-12-03 | 2020-06-11 | 无锡金农生物科技有限公司 | High-purity rice protein powder and preparation method therefor and use thereof |
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| CN1995368A (en) * | 2006-12-15 | 2007-07-11 | 江南大学 | Method for hydrolyzing, separating and preparing rice starch and rice peptide using enzyme method |
| CN103789383A (en) * | 2014-03-11 | 2014-05-14 | 江苏联海生物科技有限公司 | Rice peptide production method |
| CN107439913A (en) * | 2017-08-16 | 2017-12-08 | 寿县寿州芈八子食品科技有限公司 | Beef blood stains cleaning additive before a kind of steamed beef soup makes |
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2018
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1995368A (en) * | 2006-12-15 | 2007-07-11 | 江南大学 | Method for hydrolyzing, separating and preparing rice starch and rice peptide using enzyme method |
| CN103789383A (en) * | 2014-03-11 | 2014-05-14 | 江苏联海生物科技有限公司 | Rice peptide production method |
| CN107439913A (en) * | 2017-08-16 | 2017-12-08 | 寿县寿州芈八子食品科技有限公司 | Beef blood stains cleaning additive before a kind of steamed beef soup makes |
Non-Patent Citations (3)
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| 刘永乐: "《稻米及其制品生产技术问答》", 31 August 2010, 科学普及出版社 * |
| 刘远: "《食品生物技术导论》", 31 August 2007, 中国农业出版社 * |
| 胡小波: "大米蛋白粉的提取 ", 《生意通》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109170561A (en) * | 2018-09-11 | 2019-01-11 | 西安禾其果商贸股份有限公司 | Selenium-rich rice extract and the preparation method and application thereof |
| WO2020113975A1 (en) * | 2018-12-03 | 2020-06-11 | 无锡金农生物科技有限公司 | High-purity rice protein powder and preparation method therefor and use thereof |
| CN110089616A (en) * | 2019-05-14 | 2019-08-06 | 江西虔农农业股份有限公司 | A kind of processing method of selenium-rich hydrolysis rice protein cream and powder |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| CB03 | Change of inventor or designer information |
Inventor after: Guo Shaoxiang Inventor after: Zhu Tonggui Inventor before: Zhu Tonggui Inventor before: Wang Bihua |
|
| CB03 | Change of inventor or designer information | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180706 |
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| RJ01 | Rejection of invention patent application after publication |