CN108184920A - A kind of black nightshade extract and its application as Genes For Plant Tolerance fungus - Google Patents
A kind of black nightshade extract and its application as Genes For Plant Tolerance fungus Download PDFInfo
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- CN108184920A CN108184920A CN201711324940.6A CN201711324940A CN108184920A CN 108184920 A CN108184920 A CN 108184920A CN 201711324940 A CN201711324940 A CN 201711324940A CN 108184920 A CN108184920 A CN 108184920A
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- extract
- black nightshade
- bacteria
- carbendazim
- grape
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of black nightshade extract, the black nightshade extract are water extract or alcohol extract, and wherein alcohol extract extracting method is：It is to weigh to crush sample 800g, impregnates plant drymeal with the ethyl alcohol of 5 times of amounts, stand 12h at room temperature；Ultrasonic extraction 2h；Decompression filters, and continuous to extract 3 times, merging filtrate is concentrated at 45 DEG C with Rotary Evaporators, after dry, preserved in 4 DEG C, is spare；Further include application of the black nightshade extract as Genes For Plant Tolerance fungus.Black nightshade extract used in the present invention is a kind of botanical biological pesticide, has the characteristics that antibacterial activity is high, antimicrobial spectrum is wide, is not easy to produce resistance, is nontoxic to humans and animals, can improve grape fruit foodsafety.
Description
Technical field
The present invention relates to field of biological pesticide, and in particular to botanical biological pesticide is more particularly to a kind of black nightshade extraction
Object and its application as Genes For Plant Tolerance fungus.
Background technology
For grape as other garden crops, the serious harm of pest and disease damage is the restriction factor that industry develops in a healthy way, common
, harm serious grape disease such as grape grey mould, white rot, anthracnose etc., causing harm for these diseases seriously affect grape
Quality and yield, the difficulty that must still do not solved in the production of China's grape, and be also the problem in worldwide grape production.
In agricultural production, it is the chemical prevention method using chemical pesticide to commonly use preventing control method, but chemical prevention is sick in the presence of grape is caused
The drug resistance of opportunistic pathogen, again residual contamination, rampant equivalent risk.Using a variety of methods alternately cooperation Synthetical prevention be pest and disease damage prevention and control and
Prevent the developing direction and the approach for avoiding risk that mycotoxin pollutes.Therefore other approach and the side of non-chemical pesticide are found
Method is the vital task of industry development and the important topic of scientific research.Plant source method is excavated from plant, including opening
Botanical pesticide, the direct utilization for finding from phytochemical lead compound, even plant leaching liquor immersion are sent out, is this hair
Open up the important content in direction.
Black nightshade (Solanum nigrum L.) is Solanaceae (Solanaceae) Solanum (Solanum), and almost various regions are equal in China
It is distributed.Happiness is born near field side, wasteland and village.It is distributed widely in temperate zone to the torrid areas in Europe, Asia, America.Complete stool enters
Medicine, can eliminating stasis to subdue swelling, it is clearing heat and detoxicating.But have no report and the research that preventing and treating grapevine diseases are applied to as biological pesticide.
Invention content
A kind of application the invention mainly solves the technical problem of providing black nightshade extract and its as Genes For Plant Tolerance fungus,
Grape foodsafety can be improved.
In order to solve the above technical problems, one aspect of the present invention is：
A kind of black nightshade extract, the black nightshade extract be water extract or alcohol extract, the preparation side of the water extract
Method is：It weighs black nightshade and crushes dry product 100g, add distilled water 1000mL, impregnate 30min.First boil with high heat, after use slow boiling instead, decoct
Boil extraction 40min.Liquid is filtered while hot with 4 layers of gauze；It repeats to extract once, concentration is concentrated into slow boiling after 2 filtrates of merging
For 1000mg/mL, 4 DEG C preserve, are spare；The alcohol extract extracting method is：It is to weigh to crush sample 800g, is measured with 5 times
Ethyl alcohol impregnates plant drymeal, stands 12h at room temperature；Ultrasonic extraction 2h；Decompression filters, continuous to extract 3 times, merging filtrate, 45
It is concentrated at DEG C with Rotary Evaporators, after dry, preserved in 4 DEG C, is spare.
A kind of application of black nightshade extract as Genes For Plant Tolerance fungus is further related to, the black nightshade extract is to following disease disease
Opportunistic pathogen has significant bacteriostatic activity, mainly including Botrytis cinerea, fruit white rot of grape bacterium, grape anthracnose and Aspergillus carbonerius
Germ.
Further, wherein the grape anthracnose is included to carbendazim highly resistance anthrax bacteria bacterial strain, the grey mold
Germ is included to carbendazim highly resistance ash arrhizus bacteria bacterial strain.
Further, a concentration of 6.25mg/mL-100mg/mL of the water extract, the alcohol extract are a concentration of
0.3125mg/mL-5mg/mL。
Further, as a concentration of 100mg/mL of water extract, the bacteriostasis rate to anthrax bacteria and white rot germ is respectively
78.77% and 64.56%；As a concentration of 5mg/mL of alcohol extract, to anthrax bacteria, white rot germ and ash arrhizus bacteria inhibiting rate point
Not Wei 80.15%, 87.54% and 18.69%, to carbendazim sensitivity anthrax bacteria bacterial strain FJZZ-62, coll-9 and coll-20
Inhibiting rate be respectively 69.44%, 75.92% and 77.08%, to highly resistance anthrax bacteria bacterial strain FJND-9, FJND-40 and
The inhibiting rate of GZSD-89 is respectively 60.83%, 84.37% and 44.12%, to carbendazim sensitivity grape grey mould bacteria strain
The inhibiting rate of GXGL-7, GXNN-6 and GXNN-7 are respectively 30.38%, 34.70% and 27.36%, to carbendazim highly resistance grape
The inhibiting rate of ash arrhizus bacteria bacterial strain HBLF-10, GXZY-4 and GXZY-7 are respectively 18.10%, 29.17% and 29.80%.
The beneficial effects of the invention are as follows：Black nightshade extract of the present invention is a kind of as the germ killing drugs of active ingredient
Botanical biological pesticide belongs to one kind of bio-pharmaceutical, meets requirement of the modern society to novel pesticide.Secondly, black nightshade extract
Have the characteristics that antibacterial activity is high, antimicrobial spectrum is wide, be not easy to produce resistance, be nontoxic to humans and animals.In addition, it is normal to grape fruit
See that pathogen has inhibition, grape fruit foodsafety can be improved.
Specific embodiment
It is described in detail with reference to preferred embodiment, so that advantages and features of the invention can be easier to by this field
Technical staff's understanding, so as to make a clearer definition of the protection scope of the present invention.
Embodiment 1：
The black nightshade of the present invention is purchased from Hui nationality's medicinal material market.Lay-by material is dried, is crushed, is placed at aeration-drying and protects
It deposits spare.
Botrytis cinerea (Botrytis cinerea), fruit white rot of grape bacterium (Coniothyrium
Diplodiella), grape anthracnose (Glomerella cingulata) and grape Aspergillus carbonerius germ (Aspergillus
Carbonarius it) is provided by the Chinese Academy of Agricultural Sciences's Plant Protection Institute.
The preparation of black nightshade Aqueous extracts weighs black nightshade and crushes dry product 100g, add distilled water 1000mL, impregnates 30min.First use force
Fire boils, after use slow boiling instead, decoct extraction 40min.Liquid is filtered while hot with 4 layers of gauze.Repeat to extract primary, 2 filters of merging
After liquid a concentration of 1000mg/mL is concentrated into slow boiling.4 DEG C preserve, are spare.
The preparation of alcohol extract, which weighs, crushes sample 800g, impregnates plant drymeal with the ethyl alcohol of 5 times of amounts, stands 12h at room temperature；
Ultrasonic extraction 2h；Decompression filters, and continuous to extract 3 times, merging filtrate is concentrated at 45 DEG C with Rotary Evaporators, after dry, 4 DEG C
It is middle to preserve, is spare.
Black nightshade water extract, alcohol extract are measured to Botrytis cinerea (Botrytis using mycelial growth rate method
Cinerea), fruit white rot of grape bacterium (Coniothyrium diplodiella), grape anthracnose (Glomerella
) and the bacteriostatic activity of Aspergillus carbonerius (Aspergillus carbonarius) cingulata.It is as follows：
Strain will be frozen to recover at room temperature, with a small amount of bacterium colony picking of transfer needle picking to the culture dish containing PDA culture medium
Central area, sealing, no light inversion are incubated in the incubator of 25 DEG C of temperature, humidity 73%.
With sterile water by water extract stoste be configured to respectively 1000mg/mL, 500mg/mL, 250mg/mL, 125mg/mL,
62.5mg/mL solution.Sterile water is as blank control, and the carbendazim of 5 μ g/mL is as positive control.It works in aseptic
In platform, by prepared liquid respectively with PDA culture medium according to 1:9 ratio mixing, makes a concentration of 100 with medicine culture medium
Mg/mL, 50mg/mL, 25mg/mL, 12.5mg/mL, 6.25mg/mL, pour into the culture dish of a diameter of 90mm, and placement treats that it is solidifying
Gu.
A certain amount of extract is weighed, alcohol extract is configured to 50mg/mL, 25mg/mL, 12.5mg/ respectively with sterile water
The solution of mL, 6.25mg/mL, 3.125mg/mL.Sterile water is as blank control, and the carbendazim of 5 μ g/mL is as positive control.
In aseptic workbench, by prepared liquid respectively with PDA culture medium according to 1:9 ratio mixing, makes band medicine culture
A concentration of 5mg/mL, 2.5mg/mL, 1.25mg/mL, 0.625mg/mL, 0.3125mg/mL of base, pour into a diameter of 90mm's
Its solidification is treated in culture dish, placement.
After actication of culture, vigorous place is grown in mycelia with aseptic card punch (Ф=5mm) and uniformly punched, bacteria cake is made,
It is inoculated on band medicine culture medium, each culture dish center connects a bacteria cake, in the culture that temperature is 25-28 DEG C, humidity is 73%
After culture 2-7d is inverted in case, colony diameter is surveyed with crossing method, calculates inhibiting rate.Each processing sets 3 repetitions.
The result shows that water extract is better than white rot germ to the fungistatic effect of anthrax bacteria, to Aspergillus carbonerius germ and grey mold
Germ unrestraint acts on.As a concentration of 100mg/mL of water extract, the bacteriostasis rate to anthrax bacteria and white rot germ is respectively
78.77% and 64.56%.
Alcohol extract shows different effects to 4 kinds of grape disease fungus.As a concentration of 5mg/mL of alcohol extract, to anthrax
Germ, white rot germ and ash arrhizus bacteria inhibiting rate are respectively 80.15%, 87.54% and 18.69%.Do not have to Aspergillus carbonerius germ
There is inhibiting effect.
As a concentration of 5mg/mL of alcohol extract, to carbendazim sensitivity anthrax bacteria bacterial strain FJZZ-62, coll-9 and coll-
20 inhibiting rate is respectively 69.44%, 75.92% and 77.08%.
The carbendazim of a concentration of 100 μ g/mL is respectively to the inhibiting rate of bacterial strain FJND-9, FJND-40 and GZSD-89
9.80%th, 21.50% and 10.01%.Black nightshade alcohol extract has stronger inhibiting effect to carbendazim highly resistance anthrax bacteria bacterial strain.
As a concentration of 5mg/mL of alcohol extract, the inhibiting rate of highly resistance anthrax bacteria bacterial strain FJND-9, FJND-40 and GZSD-89 are distinguished
It is 60.83%, 84.37% and 44.12%, it is best to the inhibiting effect of FJND-40, hence it is evident that better than 100 μ g/mL carbendazim.
The inhibiting effect of 0.625mg/mL alcohol extracts is suitable with 100 μ g/mL carbendazim.
As a concentration of 5mg/mL of alcohol extract, to carbendazim sensitivity grape grey mould bacteria strain GXGL-7, GXNN-6 and
The inhibiting rate of GXNN-7 is respectively 30.38%, 34.70% and 27.36%.
The carbendazim of a concentration of 100 μ g/mL is respectively to the inhibiting rate of bacterial strain HBLF-10, GXZY-4 and GXZY-7
53.45%th, 55.11% and 14.76%.As a concentration of 5mg/mL of alcohol extract, to carbendazim highly resistance grape grey mould bacteria strain
The inhibiting rate of HBLF-10, GXZY-4 and GXZY-7 are respectively 18.10%, 29.17% and 29.80%, and effect is not as good as carbendazim.
The foregoing is merely the embodiment of the present invention, are not intended to limit the scope of the invention, every to utilize this hair
The equivalent structure or equivalent flow shift that bright description is made directly or indirectly is used in other relevant technology necks
Domain is included within the scope of the present invention.
Claims (5)
1. a kind of black nightshade extract, it is characterised in that：The black nightshade extract be water extract or alcohol extract, the water extract
Preparation method be：Weigh black nightshade and crush dry product 100g, add distilled water 1000mL, impregnate 30min, first boil with high heat, after change
With slow boiling, extraction 40min is decocted, liquid is filtered while hot with 4 layers of gauze；It repeats to extract once, uses slow boiling dense after merging 2 filtrates
A concentration of 1000mg/mL is reduced to, 4 DEG C preserve, is spare；The alcohol extract extracting method is：It is to weigh to crush sample 800g, uses
The ethyl alcohol of 5 times of amounts impregnates plant drymeal, stands 12h at room temperature；Ultrasonic extraction 2h；Decompression filters, continuous to extract 3 times, merges filter
Liquid is concentrated at 45 DEG C with Rotary Evaporators, after dry, preserved in 4 DEG C, is spare.
A kind of 2. application of black nightshade extract as described in claim 1 as Genes For Plant Tolerance fungus, which is characterized in that the dragon
Certain herbaceous plants with big flowers extract has significant bacteriostatic activity to following disease pathogen, mainly including Botrytis cinerea, fruit white rot of grape bacterium, Portugal
Grape anthrax bacteria and Aspergillus carbonerius germ.
3. application as claimed in claim 2, which is characterized in that the wherein described grape anthracnose is included to carbendazim highly resistance
Anthrax bacteria bacterial strain, the ash arrhizus bacteria are included to carbendazim highly resistance ash arrhizus bacteria bacterial strain.
4. application as claimed in claim 2 or claim 3, which is characterized in that a concentration of 6.25mg/mL-100mg/ of the water extract
ML, a concentration of 0.3125mg/mL-5mg/mL of the alcohol extract.
5. application as claimed in claim 4, which is characterized in that as a concentration of 100mg/mL of water extract, to anthrax bacteria and
The bacteriostasis rate of white rot germ is respectively 78.77% and 64.56%；As a concentration of 5mg/mL of alcohol extract, to anthrax bacteria, white rot
Germ and ash arrhizus bacteria inhibiting rate are respectively 80.15%, 87.54% and 18.69%, to carbendazim sensitivity anthrax bacteria bacterial strain
The inhibiting rate of FJZZ-62, coll-9 and coll-20 are respectively 69.44%, 75.92% and 77.08%, to highly resistance anthrax bacteria
The inhibiting rate of bacterial strain FJND-9, FJND-40 and GZSD-89 are respectively 60.83%, 84.37% and 44.12%, quick to carbendazim
The inhibiting rate for feeling grape grey mould bacteria strain GXGL-7, GXNN-6 and GXNN-7 is respectively 30.38%, 34.70% and
27.36%, the inhibiting rate to carbendazim highly resistance grape grey mould bacteria strain HBLF-10, GXZY-4 and GXZY-7 is respectively
18.10%th, 29.17% and 29.80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711324940.6A CN108184920B (en) | 2017-12-13 | 2017-12-13 | Solanum nigrum extract and application thereof as plant antibacterial |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711324940.6A CN108184920B (en) | 2017-12-13 | 2017-12-13 | Solanum nigrum extract and application thereof as plant antibacterial |
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| Publication Number | Publication Date |
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| CN108184920A true CN108184920A (en) | 2018-06-22 |
| CN108184920B CN108184920B (en) | 2023-01-06 |
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| CN201711324940.6A Active CN108184920B (en) | 2017-12-13 | 2017-12-13 | Solanum nigrum extract and application thereof as plant antibacterial |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577844A (en) * | 2016-11-15 | 2017-04-26 | 中国农业科学院麻类研究所 | Preparation for prevention and cure of pepper phytophthora blight, and production and application thereof |
-
2017
- 2017-12-13 CN CN201711324940.6A patent/CN108184920B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577844A (en) * | 2016-11-15 | 2017-04-26 | 中国农业科学院麻类研究所 | Preparation for prevention and cure of pepper phytophthora blight, and production and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 冯俊涛 等: ""56种植物抑菌活性筛选试验"", 《西北农林科技大学学报(自然科学版)》 * |
| 姬妍茹 等: ""龙葵等19种野生植物提取物的离体抑菌活性研究"", 《中国农学通报》 * |
| 赵锦慧 等: ""龙葵水提物对2种常见致病菌的抑制作用研究"", 《周口师范学院学报》 * |
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| CN108184920B (en) | 2023-01-06 |
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