CN108142443A - A kind of Herba Asari extract and its application as Genes For Plant Tolerance fungus - Google Patents
A kind of Herba Asari extract and its application as Genes For Plant Tolerance fungus Download PDFInfo
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- CN108142443A CN108142443A CN201711325207.6A CN201711325207A CN108142443A CN 108142443 A CN108142443 A CN 108142443A CN 201711325207 A CN201711325207 A CN 201711325207A CN 108142443 A CN108142443 A CN 108142443A
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- 241000233866 Fungi Species 0.000 title claims abstract description 8
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 48
- 235000019441 ethanol Nutrition 0.000 claims abstract description 45
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 241000196324 Embryophyta Species 0.000 claims abstract description 14
- 241000228212 Aspergillus Species 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000000706 filtrate Substances 0.000 claims abstract description 6
- 230000006837 decompression Effects 0.000 claims abstract description 4
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 4
- 241000894006 Bacteria Species 0.000 claims description 32
- 241000219095 Vitis Species 0.000 claims description 29
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 28
- 230000002401 inhibitory effect Effects 0.000 claims description 28
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 27
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 27
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 25
- 239000006013 carbendazim Substances 0.000 claims description 25
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 25
- 230000001580 bacterial effect Effects 0.000 claims description 22
- 241000758794 Asarum Species 0.000 claims description 18
- 241000193738 Bacillus anthracis Species 0.000 claims description 18
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 9
- 201000010099 disease Diseases 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 7
- 230000035945 sensitivity Effects 0.000 claims description 7
- 238000009835 boiling Methods 0.000 claims description 6
- 235000013399 edible fruits Nutrition 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 4
- 244000052769 pathogen Species 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 4
- 241000123650 Botrytis cinerea Species 0.000 claims description 3
- 230000003385 bacteriostatic effect Effects 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 230000001717 pathogenic effect Effects 0.000 claims description 2
- 230000001629 suppression Effects 0.000 claims description 2
- RWQKHEORZBHNRI-BMIGLBTASA-N ochratoxin A Chemical compound C([C@H](NC(=O)C1=CC(Cl)=C2C[C@H](OC(=O)C2=C1O)C)C(O)=O)C1=CC=CC=C1 RWQKHEORZBHNRI-BMIGLBTASA-N 0.000 abstract description 16
- 239000000575 pesticide Substances 0.000 abstract description 9
- 240000000560 Citrus x paradisi Species 0.000 abstract description 3
- 241001465754 Metazoa Species 0.000 abstract description 2
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 2
- 230000000845 anti-microbial effect Effects 0.000 abstract description 2
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 238000001228 spectrum Methods 0.000 abstract description 2
- VYLQGYLYRQKMFU-UHFFFAOYSA-N Ochratoxin A Natural products CC1Cc2c(Cl)cc(CNC(Cc3ccccc3)C(=O)O)cc2C(=O)O1 VYLQGYLYRQKMFU-UHFFFAOYSA-N 0.000 description 14
- DAEYIVCTQUFNTM-UHFFFAOYSA-N ochratoxin B Natural products OC1=C2C(=O)OC(C)CC2=CC=C1C(=O)NC(C(O)=O)CC1=CC=CC=C1 DAEYIVCTQUFNTM-UHFFFAOYSA-N 0.000 description 14
- 239000003053 toxin Substances 0.000 description 7
- 231100000765 toxin Toxicity 0.000 description 7
- 108700012359 toxins Proteins 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 230000006378 damage Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000001408 fungistatic effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000758795 Aristolochiaceae Species 0.000 description 2
- SLLMHZXMVHNZOR-UHFFFAOYSA-N Kakuol Chemical compound C1=C(O)C(C(=O)CC)=CC2=C1OCO2 SLLMHZXMVHNZOR-UHFFFAOYSA-N 0.000 description 2
- 235000009392 Vitis Nutrition 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005374 membrane filtration Methods 0.000 description 2
- 244000039328 opportunistic pathogen Species 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241001289295 Asarum sieboldii Species 0.000 description 1
- 241000125121 Aspergillus carbonarius Species 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 241001465180 Botrytis Species 0.000 description 1
- 241000688200 Cingulata Species 0.000 description 1
- 241000326334 Coniella diplodiella Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241001620302 Glomerella <beetle> Species 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 235000002594 Solanum nigrum Nutrition 0.000 description 1
- 240000002307 Solanum ptychanthum Species 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- HQVFCQRVQFYGRJ-UHFFFAOYSA-N formic acid;hydrate Chemical compound O.OC=O HQVFCQRVQFYGRJ-UHFFFAOYSA-N 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- -1 iginates Species 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 150000002611 lead compounds Chemical class 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 210000003739 neck Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 235000017807 phytochemicals Nutrition 0.000 description 1
- 229930000223 plant secondary metabolite Natural products 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of Herba Asari extract, the Herba Asari extract is water extract or alcohol extract, and wherein alcohol extract extracting method is：It is to weigh to crush sample 800g, impregnates plant drymeal with the ethyl alcohol of 5 times of amounts, stand 12h at room temperature；Ultrasonic extraction 2h；Decompression filters, and continuous to extract 3 times, merging filtrate is concentrated at 45 DEG C with Rotary Evaporators, after dry, preserved in 4 DEG C, is spare；Application of the Herba Asari extract as Genes For Plant Tolerance fungus is further included, can significantly inhibit the generation of Aspergillus carbonerius ochratoxin A (OTA) including it.Herba Asari extract used in the present invention is a kind of botanical biological pesticide, has the characteristics that antibacterial activity is high, antimicrobial spectrum is wide, is not easy to produce resistance, is nontoxic to humans and animals, can improve grape fruit foodsafety.
Description
Technical field
The present invention relates to field of biological pesticide, and in particular to botanical biological pesticide is more particularly to a kind of asarum extraction
Object and its application as Genes For Plant Tolerance fungus.
Background technology
Grape is one of most ancient, the most wide fruit of distribution in the world.Its cultivation history is of long standing and well established, and yield accounts for about generation
The a quarter of boundary's fruit total output.The whole world is distributed it has been reported that more than 70 kinds vitis spps, grape variety more than 8000 are planted
In temperate zone and subtropical zone, generally 20 ° -52 ° of north latitude, 30 ° -45 ° of south latitude.According to geographic origin difference, grape be divided into North America,
Eurasian and three, East Asia population.China is one of vitis spp resource distribution country the abundantest, known kind of East Asia population
Class has 42 kinds of China to have, also 1 subspecies, 12 mutation.The grape variety of plantation is there are about 500 kinds, grape -growing areas
The whole world second is occupied, is only second to Spain.2016, China's grape area more than 800,000 hectares (12,400,000 mu), ate Portugal raw
Grape yield is sure to occupy first place in the world always from after 2000.
Since grape shape flavor is all good and full of nutrition, and cultivating grape obtains good economic benefit in the past 20 years
And social benefit, China's grape industry development are rapid.But grape is as other garden crops, the serious harm of pest and disease damage
It is the restriction factor that industry develops in a healthy way, common, harm serious grape disease such as grape grey mould, white rot, anthracnose
Deng；Causing harm for these diseases seriously affects grape quality and yield, the difficulty that must still do not solved in the production of China's grape, and
And it is also problem in the production of worldwide grape.In addition, mycotoxin is to influence food security key factor, most important one
There is Ochratoxin A (OTA) toxin that Aspergillus carbonerius germ generates；The content of toxins is exceeded to cause human body very big wound
It is harmful or even carcinogenic.In agricultural production, it is using chemical pesticide to commonly use preventing control method, but chemical prevention exists and causes grape sick
The drug resistance of opportunistic pathogen, again residual contamination, rampant equivalent risk.Therefore the vital task that non-chemical pesticide is industry development is found,
It is the important topic of scientific research.Plant source method is excavated from plant, including exploitation botanical pesticide, from phytochemical
The direct utilization of middle searching lead compound, even plant leaching liquor immersion is the important content of this developing direction.
Asarum (Asarum sieboldii Miq.) is Aristolochiaceae (Aristolochiaceae) asarum (Asarum)
Plant originates in Shandong, Anhui, Zhejiang, Jiangxi, Henan, Hubei, Shaanxi, Sichuan, is born in dark and damp under height above sea level 1200-2100 Milins
In peat.Japan and Korea also have.This kind of all herbal medicine.But it has no and is applied to preventing and treating grapevine diseases as biological pesticide
Report and research.
Invention content
A kind of application the invention mainly solves the technical problem of providing Herba Asari extract and its as Genes For Plant Tolerance fungus,
Comparatively ideal control effect can be reached, and there is significant inhibiting effect to Aspergillus carbonerius production OTA abilities, grape food can be improved
Product safety.
In order to solve the above technical problems, the technical solution adopted by the present invention is：
A kind of Herba Asari extract, the Herba Asari extract be water extract or alcohol extract, the water extract extracting method
For：It weighs asarum and crushes dry product 100g, add distilled water 1000mL, impregnate 30min.First boil with high heat, after use slow boiling instead, decoct
40min is extracted, liquid is filtered while hot with 4 layers of gauze, repeats to extract once, is concentrated into after 2 filtrates of merging with slow boiling a concentration of
1000mg/mL, 4 DEG C preserve, are spare；The alcohol extract extracting method is：It is to weigh to crush sample 800g, with the second of 5 times of amounts
Alcohol impregnates plant drymeal, stands 12h at room temperature；Ultrasonic extraction 2h；Decompression filters, continuous to extract 3 times, merging filtrate, at 45 DEG C
It is lower to be concentrated with Rotary Evaporators, after dry, preserved in 4 DEG C, is spare.
A kind of application of Herba Asari extract as Genes For Plant Tolerance fungus is further related to, the Herba Asari extract is to following disease disease
Opportunistic pathogen has significant bacteriostatic activity, mainly including Botrytis cinerea, fruit white rot of grape bacterium, grape anthracnose and Aspergillus carbonerius
Germ.
Further, wherein the grape anthracnose is included to carbendazim highly resistance anthrax bacteria bacterial strain, the grey mold
Germ is included to carbendazim highly resistance ash arrhizus bacteria bacterial strain.
Further, a concentration of 6.25mg/mL-100mg/mL of the water extract, the alcohol extract are a concentration of
0.3125mg/mL-5mg/mL。
Further, when water extract is 100mg/mL, the bacteriostasis rate to anthrax bacteria, white rot germ and ash arrhizus bacteria is
40.40%th, 74.87% and 37.50%；It is 97.13% to ash arrhizus bacteria bacteriostasis rate as a concentration of 5mg/mL of alcohol extract, it is right
White rot germ, anthrax bacteria, Aspergillus carbonerius germ inhibiting rate are up to 100%.As a concentration of 2.5mg/mL of alcohol extract, to 4
The bacteriostasis rate of kind pathogen is more than 85.5%；As a concentration of 5mg/mL of alcohol extract, to carbendazim sensitivity anthrax bacteria bacterium
Strain FJZZ-62, coll-9, coll-20 bacteriostasis rate is respectively 96.30%, 94.33% and 96.88%, to carbendazim highly resistance anthrax
Germ bacterial strain FJND-9, GZSD-89 inhibiting rate are up to 100%, also reach 96.09% to the inhibiting rate of FJND-40, to more bacterium
Sensitive sense ash arrhizus bacteria bacterial strain GXGL-7, GXNN-7 germ bacterium bacteriostasis rate is up to 100%, is to GXNN-6 bacteriostasis rates
96.21%, 100% is up to carbendazim highly resistance ash arrhizus bacteria bacterial strain HBLF-10, GXZY-7 inhibiting rate, the suppression to GXZY-4
Rate processed is up to 97.01%.
Further, the alcohol extract generates OTA to Aspergillus carbonerius germ and significantly inhibits, when alcohol extract is dense
When spending for 5mg/mL, it is most apparent that OTA generates lag
The beneficial effects of the invention are as follows：Herba Asari extract of the present invention is a kind of as the germ killing drugs of active ingredient
Botanical biological pesticide belongs to one kind of bio-pharmaceutical, meets requirement of the modern society to novel pesticide.Secondly, Herba Asari extract
Have the characteristics that antibacterial activity is high, antimicrobial spectrum is wide, be not easy to produce resistance, be nontoxic to humans and animals.In addition, it is not only to grape fruit
Real encountered pathogenic bacteria has inhibition, also has significant inhibiting effect to Aspergillus carbonerius production OTA abilities, can improve grape fruit
Real foodsafety.
Specific embodiment
It is described in detail with reference to preferred embodiment, so that advantages and features of the invention can be easier to by this field
Technical staff's understanding, so as to make a clearer definition of the protection scope of the present invention.
Embodiment 1：
First, the preparation of water extract and alcohol extract
The asarum of the present invention is purchased from Hui nationality's medicinal material market.Lay-by material is dried, is crushed, is placed at aeration-drying and protects
It deposits spare.
Water extract extracting method is：It weighs black nightshade and crushes dry product 100g, add distilled water 1000mL, impregnate 30min.First use force
Fire boils, after use slow boiling instead, decoct extraction 40min.Liquid is filtered while hot with 4 layers of gauze.Repeat to extract primary, 2 filters of merging
After liquid a concentration of 1000mg/mL is concentrated into slow boiling.4 DEG C preserve, are spare.Asarum alcohol extracting step is：It is to weigh crushing sample
800g impregnates plant drymeal with the ethyl alcohol of 5 times of amounts, stands 12h at room temperature；Ultrasonic extraction 2h；Decompression filters, continuous to extract 3 times,
Merging filtrate is concentrated at 45 DEG C with Rotary Evaporators, after dry, preserved in 4 DEG C, is spare.
2nd, the detection method of micro-organisms and OTA
Asarum water extract, alcohol extract are measured to Botrytis cinerea (Botrytis using mycelial growth rate method
Cinerea), fruit white rot of grape bacterium (Coniothyrium diplodiella), grape anthracnose (Glomerella
) and the bacteriostatic activity of Aspergillus carbonerius (Aspergillus carbonarius) cingulata.It is as follows：
After actication of culture, vigorous place is grown in culture dish edge mycelia with aseptic card punch (Ф=5mm) and uniformly punched,
Bacteria cake is made, is inoculated on culture medium, each culture dish center connects a bacteria cake, temperature is 25-28 DEG C, humidity is 73%
After culture 2-7d is inverted in incubator, colony diameter is surveyed with crossing method, calculates inhibiting rate.Each processing sets 3 repetitions.
Strain cultivates 14d on CYA culture mediums, plays the training for taking 12 pieces of carriers with aseptic card punch (Φ=5mm) daily
Support object.The culture that will carry disease germs is put in 5mL small test tubes, and methanol 2mL, whirlpool concussion 1min, static extraction 60min are added in after weighing
Afterwards, it first, then with 0.22 μm of membrane filtration, is finally examined with 0.45 μm of membrane filtration with ultra high efficiency liquid phase-cascade mass spectrometer
It surveys.
Ultra high efficiency liquid phase-string mass spectrograph testing conditions：Using the superelevation liquid chromatograph XevoTM TQ-S of U.S. Water
It is programmed simultaneously automatic sampling.Mobile phase is A1：Chromatography acetonitrile；A2：Chromatography methanol；B1：0.2% formic acid water；B2：Water；Flow velocity
It is set as：0.3mL/min；Sample size：1μL.Every gram is calculated according to the quality of taken agar block and the content of toxins detected
Toxin concentration in culture is expressed as Output of toxin (ng/g).
3rd, the bacteriostasis of water extract and alcohol extract
The result shows that asarum water extract is 74.87% to the bacteriostasis rate of white rot bacterium, fungistatic effect better than anthrax bacteria,
Ash arrhizus bacteria, and there is no inhibiting effect to Aspergillus carbonerius germ.
Asarum alcohol extract shows preferable fungistatic effect to 4 kinds of grape disease fungus.When asarum alcohol extract is a concentration of
It is 97.13% to ash arrhizus bacteria bacteriostasis rate during 5mg/mL, it is high to white rot bacterium, anthrax bacteria, Aspergillus carbonerius germ inhibiting rate
Up to 100%.As a concentration of 2.5mg/mL of alcohol extract, to the bacteriostasis rates of 4 kinds of pathogens more than 85.5%, EC50 values are
0.48-1.10mg/mL.The fungistatic effect of asarum alcohol extract dialogue maize ear rot bacterium is best, as a concentration of 1.25mg/mL of alcohol extract,
98.08% is up to the inhibiting rate of white rot bacterium.
4th, alcohol extract is to the inhibiting effect of carbendazim sensitivity anthrax bacteria bacterial strain
As a concentration of 5mg/mL of asarum alcohol extract, to carbendazim sensitivity anthrax bacteria bacterial strain FJZZ-62, coll-9,
Coll-20 bacteriostasis rates are respectively 96.30%, 94.33% and 96.88%, EC50 are respectively 0.52,0.92 and 0.46mg/mL.
5th, alcohol extract is to the inhibiting effect of carbendazim highly resistance anthrax bacteria bacterial strain
Asarum alcohol extract goes out good inhibiting effect to carbendazim highly resistance anthrax bacteria strains expressed.When alcohol extract is a concentration of
During 5mg/mL, 100% is up to carbendazim highly resistance anthrax bacteria bacterial strain FJND-9, GZSD-89 inhibiting rate, to FJND-40's
Inhibiting rate also reaches 96.09%.EC50 is respectively 0.46,1.39 and 0.77mg/mL.And 100 μ g/mL carbendazim to FJND-9,
The inhibiting rate of FJND-40 and GZSD-89 bacterial strains is respectively 9.80%, 21.50% and 10.01%.When alcohol extract is a concentration of
During 0.625mg/mL, to the inhibitory activity of the highly resistance bacterial strain also obvious activity for being better than 100 μ g/mL carbendazim.
6th, alcohol extract is to the inhibiting effect of carbendazim sensitivity ash arrhizus bacteria bacterial strain
During a concentration of 5mg/mL of asarum alcohol extract, to carbendazim sensitivity ash arrhizus bacteria bacterial strain GXGL-7, GXNN-7 germ bacterium
It is 1.06-1.59mg/mL's that bacteriostasis rate up to 100%, which is 96.21%, EC50 to GXNN-6 bacteriostasis rates,.
7th, alcohol extract is to the inhibiting effect of carbendazim highly resistance ash arrhizus bacteria bacterial strain
A concentration of 100 μ g/mL carbendazim is respectively to the inhibiting rate of HBLF-10, GXZY-4 and GXZY-7 bacterial strain
53.45%th, 55.11% and 14.76%.Asarum alcohol extract has carbendazim highly resistance ash arrhizus bacteria bacterial strain preferable inhibition to make
With effect is better than 100 μ g/mL carbendazim.As its a concentration of 5mg/mL, to carbendazim highly resistance ash arrhizus bacteria bacterial strain HBLF-
10th, GXZY-7 inhibiting rates are up to 100%, to the inhibiting rate of GXZY-4 up to 97.01%.Its EC50 is 1.04-1.34mg/mL.
8th, alcohol extract is to the inhibiting effect of Aspergillus Niger toxin
In blank control, OTA starts to generate in 3d, and a concentration of 6.22ng/g, 9d OTA yield peaks, dense
It spends for 2970.78ng/g.After culture medium adds asarum alcohol extract, there is retardation compared with blank control in the generation of OTA.Kakuol
Extract concentration is higher, and the time that OTA is generated more lags, and toxin producing is fewer.During a concentration of 0.3125mg/mL of alcohol extract, 5d is then
Begin with OTA generations, a concentration of 196.44ng/g of OTA.During a concentration of 5mg/mL of alcohol extract, OTA yield lags most apparent, 9d
Just there is a small amount of OTA to generate, when OTA a concentration of 31.56ng/g, 14d is 563.78ng/g, hence it is evident that less than control.
The foregoing is merely the embodiment of the present invention, are not intended to limit the scope of the invention, every to utilize this hair
The equivalent structure or equivalent flow shift that bright description is made directly or indirectly is used in other relevant technology necks
Domain is included within the scope of the present invention.
Claims (6)
1. a kind of Herba Asari extract, it is characterised in that：The Herba Asari extract be water extract or alcohol extract, the water extract
Extracting method is：Weigh asarum and crush dry product 100g, add distilled water 1000mL, impregnate 30min, first boil with high heat, after use instead
Slow boiling decocts extraction 40min, and liquid is filtered while hot with 4 layers of gauze, repeats to extract once, be concentrated after merging 2 filtrates with slow boiling
To a concentration of 1000mg/mL, 4 DEG C preserve, are spare；The alcohol extract extracting method is：It weighs and crushes sample 800g, with 5 times
The ethyl alcohol of amount impregnates plant drymeal, stands 12h at room temperature；Ultrasonic extraction 2h；Decompression filters, continuous to extract 3 times, merging filtrate,
It is concentrated at 45 DEG C with Rotary Evaporators, after dry, preserved in 4 DEG C, is spare.
2. application of a kind of Herba Asari extract as described in claim 1 as Genes For Plant Tolerance fungus, which is characterized in that described is thin
Pungent extract has significant bacteriostatic activity to following disease pathogen, mainly including Botrytis cinerea, fruit white rot of grape bacterium, Portugal
Grape anthrax bacteria and Aspergillus carbonerius germ.
3. application as claimed in claim 2, which is characterized in that the wherein described grape anthracnose is included to carbendazim highly resistance
Anthrax bacteria bacterial strain, the ash arrhizus bacteria are included to carbendazim highly resistance ash arrhizus bacteria bacterial strain.
4. application as claimed in claim 2 or claim 3, which is characterized in that a concentration of 6.25mg/mL-100mg/ of the water extract
ML, a concentration of 0.3125mg/mL-5mg/mL of the alcohol extract.
5. application as claimed in claim 4, which is characterized in that when water extract is 100mg/mL, to anthrax bacteria, white rot
The bacteriostasis rate of bacterium and ash arrhizus bacteria is 40.40%, 74.87% and 37.50%；As a concentration of 5mg/mL of alcohol extract, to grey mold
Germ bacteriostasis rate is 97.13%, and 100% is up to white rot bacterium, anthrax bacteria, Aspergillus carbonerius germ inhibiting rate；Work as alcohol extracting
During a concentration of 2.5mg/mL of object, to the bacteriostasis rates of 4 kinds of pathogens more than 85.5%；As a concentration of 5mg/mL of alcohol extract,
It is respectively 96.30%, 94.33% and to carbendazim sensitivity anthrax bacteria bacterial strain FJZZ-62, coll-9, coll-20 bacteriostasis rate
96.88%, 100% is up to carbendazim highly resistance anthrax bacteria bacterial strain FJND-9, GZSD-89 inhibiting rate, the suppression to FJND-40
Rate processed also reaches 96.09%, and carbendazim sensitivity ash arrhizus bacteria bacterial strain GXGL-7, GXNN-7 germ bacterium bacteriostasis rate is up to
100%, it is 96.21% to GXNN-6 bacteriostasis rates, it is equal to carbendazim highly resistance ash arrhizus bacteria bacterial strain HBLF-10, GXZY-7 inhibiting rate
Up to 100%, to the inhibiting rate of GXZY-4 up to 97.01%.
6. application as claimed in claim 4, which is characterized in that the alcohol extract generates OTA to Aspergillus carbonerius germ to be had
Apparent inhibiting effect, as a concentration of 5mg/mL of alcohol extract, it is most apparent that OTA generates lag.
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| CN101027990A (en) * | 2007-03-13 | 2007-09-05 | 西北农林科技大学无公害农药研究服务中心 | Bactericidal use of asarone for preventing and treating plant diseases caused by plant pathogenic bacteria |
| CN105941513A (en) * | 2016-06-26 | 2016-09-21 | 合肥慧谷农业科技有限公司 | Plant bactericide and preparation method thereof |
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| CN105941513A (en) * | 2016-06-26 | 2016-09-21 | 合肥慧谷农业科技有限公司 | Plant bactericide and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 刘海燕: "细辛挥发油抗植物病原真菌活性及作用机理研究", 《中国优秀博硕士学位论文全文数据库 (硕士)医药卫生科技辑》 * |
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| CN111778165B (en) * | 2020-08-11 | 2022-10-25 | 哈尔滨工业大学 | Aspergillus niger DFY1 and application thereof |
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