CN108157608B

CN108157608B - Anti-stress and growth-promoting feed additive

Info

Publication number: CN108157608B
Application number: CN201810196935.XA
Authority: CN
Inventors: 肖世平; 魏伟群
Original assignee: Jiangxi Tianjia Biological Engineering Co ltd
Current assignee: Jiangxi Tianjia Biological Engineering Co ltd
Priority date: 2018-03-10
Filing date: 2018-03-10
Publication date: 2021-06-08
Anticipated expiration: 2038-03-10
Also published as: CN108157608A

Abstract

The invention relates to a feed additive for resisting stress and promoting growth, belonging to the field of feed additives. Every 100 parts of the feed additive comprise the following raw materials in parts by weight: 2-6 parts of gamma-aminobutyric acid, 4-8 parts of taurine, 3-8 parts of almond extract, 5-9 parts of refined composite plant seed powder, 7-12 parts of pleurotus nebrodensis and the balance of a carrier, wherein the carrier is wheat bran and medical stone in a mass ratio of 1-2: 1. The feed additive can effectively relieve the stress reaction of the cultured animals, reduce the death rate, effectively promote the growth and the production performance of the cultured animals, improve the health condition of the organisms of the cultured animals, reduce the feed conversion rate and improve the feed conversion rate.

Description

Anti-stress and growth-promoting feed additive

The technical field is as follows: the invention relates to a feed additive, in particular to a feed additive for resisting stress and promoting growth.

Background art:

the stress is a series of nonspecific systemic reactions or the sum of nonspecific reactions generated immediately after animals are acted on the body by some injurious biological, physical, chemical and special psychological strong stimuli (stress factors) in external and internal environments, and the stress can obviously injure the digestive system, respiratory system and immune system of the animals, so that the metabolic disorder of the body is caused, and the physiological function, the production performance and even the life crisis of the animals are directly influenced.

The existing anti-stress aspect of breeding animals is usually to change the breeding method and improve the environment or add electrolytes, vitamins, trace elements and the like, so the effect is not ideal, the breeding cost is also improved, the requirement of the animals on rapid growth and development cannot be met, and the stress reaction of the animals in transportation, production and breeding can be resisted.

Therefore, the development of the feed additive can effectively promote the cultured animals to resist various adverse environments and stress caused by feeding management, improve the health condition of the cultured animals, promote growth, and is beneficial to the healthy and continuous development of the animal breeding industry, thereby generating great economic benefit.

The invention content is as follows:

the invention aims to provide an anti-stress and growth-promoting feed additive, which can effectively relieve the stress reaction of cultured animals, reduce the death rate, effectively promote the growth and production performance of the cultured animals, improve the health condition of the cultured animals, reduce the feed conversion rate and improve the feed conversion rate.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

the feed additive with the functions of resisting stress and promoting growth is composed of the following raw materials in parts by weight per 100 parts: 2-6 parts of gamma-aminobutyric acid, 4-8 parts of taurine, 3-8 parts of almond extract, 5-9 parts of refined composite plant seed powder, 7-12 parts of pleurotus nebrodensis and the balance of a carrier, wherein the carrier is composed of wheat bran and medical stone according to the mass ratio of 1-2: 1;

the preparation method of the almond extract comprises the following steps: taking almond, crushing, sieving with a 80-mesh sieve, adding calcium chloride accounting for 0.4-0.8% of the mass of the almond and water accounting for 8-12 times of the mass of the almond, uniformly stirring, heating to 40-50 ℃, adding a complex enzyme preparation accounting for 0.3-0.7% of the mass of the almond, performing enzymolysis for 1-2h, filtering to obtain a filtrate, and performing vacuum drying on the filtrate to obtain an extract of the almond; the compound enzyme preparation consists of alpha-galactosidase, beta-glucanase and cellulase in a mass ratio of 1:1-2: 1-2;

the preparation method of the refined composite plant seed powder comprises the following steps:

(1) pulverizing fructus Carthami tinctorii, sieving with 60-80 mesh sieve, freezing at-20 deg.C for 1-2 hr, adding 1.0-1.5% sodium chloride 5-8 times the weight of fructus Carthami tinctorii, stirring, soaking for 20-30min, filtering, removing filtrate, and vacuum drying the residue to obtain fructus Carthami tinctorii residue;

(2) parching semen gossypii powder with slow fire for 10-20min, pulverizing, and sieving with 60-80 mesh sieve to obtain semen gossypii powder;

(3) pulverizing evening primrose seed, sieving with 60-80 mesh sieve, adding water 8-12 times of evening primrose seed, stirring, adding lactic acid until lactic acid concentration is 0.5-0.9%, soaking for 15-20min, filtering, removing filtrate, and vacuum drying the residue to obtain evening primrose seed residue;

(4) mixing the substances prepared in the steps (1), (2) and (3) according to the mass ratio of 1:1-2:2-3 to obtain a mixture, adding 4-6 times of water by weight of the mixture, keeping the temperature at 45-55 ℃, soaking for 0.5-0.8h, then adding 0.1-0.3% of glucose by weight of the mixture, uniformly stirring, heating to 75-85 ℃, continuing to soak for 0.8-1.2h, then adding a certain amount of ethanol until the concentration of the ethanol is 45-55%, keeping the temperature at 30-40 ℃, continuing to soak for 0.5-1h, heating to 95 ℃, stirring until the ethanol is completely volatilized, then vacuum concentrating to 1/5 of the original volume, vacuum drying, crushing and sieving with a 80-mesh sieve to obtain the refined composite seed powder.

The feed additive can be widely applied to feed for breeding animals such as pigs, chickens, ducks, fishes and the like, and the addition amount is 1-1.5 kg/ton.

Has the advantages that:

1. the preparation method of the refined composite plant seed powder is researched by the applicant in an effort, the content of effective substance components in safflower seeds, kapok seeds and evening primrose seeds can be effectively improved, and the refined composite plant seed powder is scientifically compounded with taurine and gamma-aminobutyric acid, so that the regulation effect of the taurine and the gamma-aminobutyric acid on the central nervous system of the animal can be effectively enhanced, and the capacities of resisting oxidation and removing free radicals of the animal are obviously improved;

2. the feed additive is scientifically compounded, and the components have a synergistic effect, so that the feed additive can be widely applied to breeding animals such as pigs, chickens, ducks, fishes and the like, effectively relieve stress reaction, promote the growth performance of the animals, increase the daily gain of the breeding animals by 15-40% and increase the survival rate by 10-30%;

3. in the pig growth test process, the applicant finds that the feed additive can also effectively improve the pork quality, increase the intramuscular fat content and the eye muscle area of the pork and reduce the drip loss.

Detailed Description

Example 1

The feed additive with the functions of resisting stress and promoting growth is composed of the following raw materials in parts by weight per 100 parts: 4 parts of gamma-aminobutyric acid, 6 parts of taurine, 6 parts of almond extract, 7 parts of refined composite plant seed powder, 10 parts of pleurotus nebrodensis and the balance of a carrier, wherein the carrier is composed of wheat bran and medical stone according to the mass ratio of 1.5: 1;

the preparation method of the almond extract comprises the following steps: taking almond, crushing, sieving with a 80-mesh sieve, adding calcium chloride accounting for 0.6 percent of the mass of the almond and water accounting for 10 times of the mass of the almond, uniformly stirring, heating to 45 ℃, adding a complex enzyme preparation accounting for 0.5 percent of the mass of the almond, performing enzymolysis for 1.5h, filtering to obtain a filtrate, and performing vacuum drying on the filtrate to obtain an extract of the almond;

the compound enzyme preparation consists of alpha-galactosidase, beta-glucanase and cellulase according to the mass ratio of 1:1.5: 1.5;

(1) pulverizing fructus Carthami tinctorii, sieving with 70 mesh sieve, freezing at-20 deg.C for 1.5h, adding 1.3% sodium chloride 7 times the weight of fructus Carthami tinctorii, stirring, soaking for 250min, filtering, removing filtrate, and vacuum drying the residue to obtain fructus Carthami tinctorii residue;

(2) parching semen gossypii powder with slow fire for 15min, pulverizing, and sieving with 70 mesh sieve to obtain semen gossypii powder;

(3) pulverizing evening primrose seed, sieving with 70 mesh sieve, adding water 10 times of evening primrose seed, stirring, adding certain amount of lactic acid until lactic acid concentration is 0.7%, soaking for 18min, filtering, removing filtrate, and vacuum drying the residue to obtain evening primrose seed residue;

(4) mixing the substances prepared in the steps (1), (2) and (3) according to the mass ratio of 1:1.5:2.5 to obtain a mixture, adding water which is 5 times the weight of the mixture, keeping the temperature at 50 ℃ for soaking for 0.7h, then adding glucose with the mass of 0.2% of the mixture, uniformly stirring, heating to 80 ℃, continuing to soak for 1h, then adding a certain amount of ethanol until the concentration of the ethanol is 50%, keeping the temperature at 35 ℃ for continuing to soak for 0.8h, heating to 95 ℃, stirring until the ethanol is completely volatilized, then carrying out vacuum concentration to 1/5 of the original volume, carrying out vacuum drying, crushing and sieving with a 80-mesh sieve to obtain the refined composite seed powder.

Example 2

The feed additive with the functions of resisting stress and promoting growth is composed of the following raw materials in parts by weight per 100 parts: 4 parts of gamma-aminobutyric acid, 6 parts of taurine, 6 parts of almond extract, 7 parts of compound plant seed powder, 10 parts of pleurotus nebrodensis and the balance of a carrier, wherein the carrier is composed of wheat bran and medical stone according to the mass ratio of 1.5: 1;

the composite plant seed powder consists of safflower seeds, kapok seeds and evening primrose seeds according to the mass ratio of 1:1.5: 2.5.

Example 3

The feed additive with the functions of resisting stress and promoting growth is composed of the following raw materials in parts by weight per 100 parts: 4 parts of gamma-aminobutyric acid, 6 parts of taurine, 6 parts of almond extract, 10 parts of pleurotus nebrodensis and the balance of a carrier, wherein the carrier is composed of wheat bran and medical stone according to the mass ratio of 1.5: 1;

the preparation method of the almond extract is the same as that of example 1.

Example 4

According to the patent with the application number of 201610165096.6, the disclosed compound feed additive for pigs has the advantages that the compound feed additive for pigs has the effects of stress resistance and growth promotion, and comprises the following raw materials in parts by weight:

15 parts of gamma-aminobutyric acid, 15 parts of tauroursodeoxycholic acid, 6 parts of phytosterol ester, 0.5 part of resveratrol, 4 parts of plectranthus, 6 parts of pyrola, 7 parts of scouring rush, 2 parts of chaenomeles speciosa, 8 parts of cynanchum glaucescens, 3 parts of Chinese mahonia leaves, 4 parts of szechwan chinaberry fruits, 4 parts of creeping rostellularia herb, 7 parts of patrinia heterophylla, 2 parts of pleione indica, 10 parts of lotus seeds, 2 parts of rhaponticum carthamoides, 6 parts of pozuvine fog, 3 parts of loosestrife, 5 parts of bacillus licheniformis freeze-dried powder, 7 parts of clostridium butyricum freeze-dried powder and 650 parts of a carrier.

The phytosterol ester is beta-sitosterol ester: stigmasterol ester: mixture of campesterol ester =1:1: 1;

the carrier is corn: bean pulp =6:1 mixture;

the preparation method comprises the following steps:

the Chinese herbal medicines in the formula are: herba Rabdosiae Glaucocalycis, herba Pyrolae, herba Equiseti hiemalis, fructus Chaenomelis, rhizoma Cynanchi Stauntonii, folium Mahoniae, fructus Toosendan, herba Rostellulariae, radix patriniae, Bulbus Iphigeniae Indicae, semen Cauliginis, radix seu caulis Gei Multiflorae, and herba Lysimachiae Clethroids are decocted with water twice, each time the water amount is 6 times of the raw materials, each time the decoction is boiled for 0.5-1 hr, the decoctions are mixed and filtered, the filtrate is distilled and concentrated to obtain an extract, the extract is dried and crushed, processed by 80-100 meshes, mixed with gamma-aminobutyric acid, tauroursodeoxycholic acid, phytosterol ester, resveratrol, bacillus licheniformis freeze-dried powder, clostridium butyricum freeze-dried powder and.

10kg of the additive is added into each ton of pig feed.

Test example:

1. test animals and designs

300 healthy and large three-element pigs with the average body weight of about 40kg in subordinate farms of Jiangxiangtian bioengineering GmbH are selected for experiments, and are randomly divided into 5 groups, 6 repeats in each group, 10 repeats in each group, and the groups are respectively a control group, a test 1 group, a test 2 group, a test 3 group and a test 4 group.

2. Test feed

Control group: commercial basal ration for conventional feed for growing-finishing pigs;

test 1, test 2, and test 3: the feed additives prepared in the embodiments 1, 2 and 3 are respectively added into the basic daily ration of the control group, and the addition amount is 1.2 kg/ton;

test group 4 groups: the feed additive prepared in example 4 was added to the basal diet of the control group at a dose of 10 kg/ton.

3. Feeding management and index determination

In summer, under the condition of industrial culture, the house temperature is 30-35 ℃, and the relative humidity is 60-75%. The test pigs were fed and drunk freely and were subjected to conventional feeding management. The test period is 30 days, the weight is weighed and the material is hardened after the test is finished, 6 test pigs are slaughtered randomly in each test group respectively, the eye muscle area, the tenderness, the intramuscular fat and the drip loss are measured, and serum is collected to measure the antioxidant index. And calculating the daily gain, feed intake and feed conversion ratio of each treatment group, and observing the mental condition of the pigs.

Data statistical analysis: experimental data were analyzed for one-factor anova using SSPS13.0 statistical software.

4. The test results are shown in Table 1, Table 2 and Table 3 below

TABLE 1 growth Performance index results for test pigs of different test groups

Item	Control group	Test 1 group	Test 2 groups	Test 3 groups	Test 4 groups
						Initial weight (kg)	40.24	40.32	40.6	40.23	40.21
Terminal weight (kg)	58.92	64.82	59.43	58.92	61.26
						Daily gain (g/d, head)	622.7	816.7	627.7	623.0	701.7
Daily food intake (g/d, head)	1469.5	1706.8	1468.7	1470.3	1571.7
						Meat ratio of materials	2.36	2.09	2.34	2.36	2.24

TABLE 2 pork quality index results of different test groups

Item	Control group	Test 1 group	Test 2 groups	Test 3 groups	Test 4 groups
						Area of eye muscle	36.9	48.2	37.5	37.8	36.5
Intramuscular fat	1.71	2.11	1.72	1.73	1.70
						Drip loss	2.46	2.13	2.42	2.44	2.47

TABLE 3 results of the antioxidant index of pig serum tested in different test groups

Item	Control group	Test 1 group	Test 2 groups	Test 3 groups	Test 4 groups
						Superoxide dismutase Activity (SOD) (IU/ml)	88.12	116.32	89.12	87.87	109.23
Glutathione peroxidase Activity (GSH-Px) (IU/ml)	21.21	34.18	22.32	21.81	29.14
						Malondialdehyde (MDA)	4.73	2.08	4.67	4.61	3.24

As can be seen from Table 1, the daily gain and the daily feed intake of the test pigs in the test group 1 are respectively increased by 31.2%, 30.1%, 31.1%, 16.4% and 16.1%, 16.2%, 16.1% and 8.6% compared with those in the control group, the test group 2, the test group 3 and the test group 4, and the feed-meat ratio of the test group 1 is respectively reduced by 11.4%, 10.7%, 11.4% and 6.7% compared with those in the control group, the test group 2, the test group 3 and the test group 4, which shows that the feed additive can obviously improve the growth performance of the fattening pigs.

As can be seen from table 2, in the test 1 group, compared with the control group, the test 2 group, the test 3 group and the test 4 group, the eye muscle area of pork was increased by more than 27%, the intramuscular fat was increased by more than 21%, and the drip loss was reduced by more than 11%; the pork eye muscle area, intramuscular fat and drip loss were not significantly different in the control group, test 2 group, test 3 group and test 4 group. The feed additive can effectively improve the pork quality.

As can be seen from Table 3, the superoxide dismutase activity (SOD) and the glutathione peroxidase activity (GSH-Px) of the pigs tested in the test group 1 are respectively improved by 32 percent, 30.5 percent, 32.4 percent, 17.2 percent, 37.6 percent, 30.7 percent, 33.8 percent and 16.1 percent compared with the control group, the test group 2, the test group 3 and the test group 4; and Malondialdehyde (MDA) is respectively reduced by 43.3%, 42.6%, 41.9% and 17.3%, which shows that the feed additive can effectively improve the level of the oxidation resistance index of the test pig and improve the anti-stress capability of the pig.

Claims

1. An anti-stress and growth-promoting feed additive, which is characterized in that: each 100 parts of the composition comprises the following raw materials in parts by weight: 2-6 parts of gamma-aminobutyric acid, 4-8 parts of taurine, 3-8 parts of almond extract, 5-9 parts of refined composite plant seed powder, 7-12 parts of pleurotus nebrodensis and the balance of a carrier, wherein the carrier is composed of wheat bran and medical stone according to the mass ratio of 1-2: 1;

(4) mixing the substances prepared in the steps (1), (2) and (3) according to the mass ratio of 1:1-2:2-3 to obtain a mixture, adding water with the weight of 4-6 times of the weight of the mixture, keeping the temperature at 45-55 ℃, soaking for 0.5-0.8h, then adding glucose with the mass of 0.1-0.3% of the mixture, uniformly stirring, heating to 75-85 ℃, continuing to soak for 0.8-1.2h, then adding ethanol until the concentration of the ethanol is 45-55%, keeping the temperature at 30-40 ℃, continuing to soak for 0.5-1h, heating to 95 ℃, stirring until the ethanol is completely volatilized, then vacuum concentrating to the original volume of 1/5, then vacuum drying, crushing and sieving with a 80-mesh sieve to obtain the refined composite seed powder.

2. The feed additive for resisting stress and promoting growth according to claim 1, wherein each 100 parts of the feed additive comprises the following raw materials in parts by weight: 4 parts of gamma-aminobutyric acid, 6 parts of taurine, 6 parts of almond extract, 7 parts of refined composite plant seed powder, 10 parts of pleurotus nebrodensis and the balance of a carrier, wherein the carrier is composed of wheat bran and medical stone according to the mass ratio of 1.5: 1;

the preparation method of the almond extract comprises the following steps: taking almond, crushing, sieving with a 80-mesh sieve, adding calcium chloride accounting for 0.6 percent of the mass of the almond and water accounting for 10 times of the mass of the almond, uniformly stirring, heating to 45 ℃, adding a complex enzyme preparation accounting for 0.5 percent of the mass of the almond, performing enzymolysis for 1.5h, filtering to obtain a filtrate, and performing vacuum drying on the filtrate to obtain an extract of the almond; the compound enzyme preparation consists of alpha-galactosidase, beta-glucanase and cellulase according to the mass ratio of 1:1.5: 1.5;

(3) pulverizing evening primrose seed, sieving with 70 mesh sieve, adding water 10 times of evening primrose seed, stirring, adding lactic acid until lactic acid concentration is 0.7%, soaking for 18min, filtering, removing filtrate, and vacuum drying the residue to obtain evening primrose seed residue;

(4) mixing the substances prepared in the steps (1), (2) and (3) according to the mass ratio of 1:1.5:2.5 to obtain a mixture, adding water which is 5 times the weight of the mixture, keeping the temperature at 50 ℃ for soaking for 0.7h, then adding glucose with the mass of 0.2% of the mixture, uniformly stirring, heating to 80 ℃, continuing to soak for 1h, then adding ethanol until the concentration of the ethanol is 50%, keeping the temperature at 35 ℃ for continuing to soak for 0.8h, heating to 95 ℃, stirring until the ethanol is completely volatilized, then carrying out vacuum concentration to 1/5 of the original volume, carrying out vacuum drying, crushing, and sieving with a 80-mesh sieve to obtain the refined composite seed powder.