CN108124749B - A kind of method that clematis Water culture is taken root - Google Patents
A kind of method that clematis Water culture is taken root Download PDFInfo
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- CN108124749B CN108124749B CN201711320853.3A CN201711320853A CN108124749B CN 108124749 B CN108124749 B CN 108124749B CN 201711320853 A CN201711320853 A CN 201711320853A CN 108124749 B CN108124749 B CN 108124749B
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- clematis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
A kind of method that clematis Water culture is taken root, management, acclimatization and transplants process after the preparation of preparation, mill water culture nutrient solution including cuttage vessel, the selection of clematis cuttings and processing, water planting culture, cuttage, on the basis of water culture technique, influence by plant growth regulator to clematis cuttage root-taking improves cutting plantation；Make the conditions such as moisture, temperature, illumination and air humidity controllable during cutting propagation, reduce influence of the external environment to plant propagation rate, cutting plantation is stablized, plant growth state well overcomes the problems, such as that cuttage is subject to seasonal restrictions, shorten breeding cycle, improve cuttage survival rate, cuttings survival rate is up to 85% or more, be conducive to the factorial production and popularization and application of clematis plant, there are preferable economic benefit, social benefit and ecological benefits.
Description
Technical field
The invention belongs to plant cuttage propagation fields, more particularly to a kind of method that clematis Water culture is taken root.
Background technique
Clematis (Clematis L.) is under the jurisdiction of Ranunculaceae (Ranunculaceae), is liana the most popular in recent years
One of flowers, pattern is abundant, flower pattern is different, many kinds of, possesses beautiful, graceful, generous unique ornamental value, can not only be used for
The pot flowers of novel in style, it can also be used to the ground system vertical such as wall, arched door, style greening, can also make cut-flower, by etc. answer
With leading in many world-class flower shows, " tough such as iron wire, the flowers are in blossom such as lotus " has the laudatory title of " liana queen ".In recent years,
With flourishing for home gardening and afforestation, market demand also increases year by year, and only Zhejiang rainbow in 2016 is more limited
The clematis sales volume of company increases to 5,000,000 from 800,000 in 2010, favorite of the clematis as home gardening, it is deep by
The favorite outstanding person of consumer.
As having green plants with broad prospects for development, market demand gradually increases clematis, main at this stage
There are three types of modess of reproduction: one kind is that seed propagation is sowed, but clematis seed is sprouted in the fall with the full seed of current year picking
Hair rate is lower, and most of clematis seed needs to sprout by the low temperature period of vernalization, and germinating time is longer, can not stablize
Large-scale breeding；Second is cutting propagation, and the prior art generally carries out in the fall, needs can just take root within 1-2 months, and skewer
It is unstable to be inserted into motility rate, is affected by the external environment larger, it can not production breeding；The third is mound layering, and this method uses
Last year generates ripe branch press strip, usually takes root, takes a long time in 1 year.
In general, the clematis seeding and seedling raising period is longer, and seedling quantity is unstable, in production to take cutting propagation more
Technology, but also due to cuttage survival rate is lower, slow growth influences the popularization of its large-scale breeding.Therefore, how iron wire is solved
Lotus cuttage difficult problem is the key that optimization clematis quickly breeds, is of great significance to the breeding of clematis the factorial production.
Summary of the invention
In order to overcome the shortcomings of that background technique, the present invention provide a kind of clematis Water culture rooting method, improve existing
Cultivation period is long, it is slow to take root, survival rate is unstable status.
The technical solution adopted in the present invention: a kind of clematis Water culture rooting method, including following operating procedure:
1) preparation of cuttage vessel: select opaque cabinet as cuttage vessel；
2) preparation of mill water culture nutrient solution: needed in the mill water culture nutrient solution comprising nutrient N, P, K, Ca, Mg, Fe, Zn,
Mn, Cu, Mo, B and inorganic chelator EDTA-Na2, and pH value is 5.0-5.5；
3) selection and processing of clematis cuttings: the maturation zone bud branch of the no disease and pests harm of selection semi-lignified or lignifying
As cuttings, the long 3-4cm of cuttings, top section is cross section, and lower part section is scarf, 1 blade is only stayed, by cuttings base portion
1-2cm submerges and impregnates 15min in plant growth regulator, processing of drying in the shade is taken out after immersion, wherein the plant growth regulating
Agent selects the indolebutyric acid (IBA) that concentration is 100mg/L for the methyl α-naphthyl acetate (NAA) or concentration of 200mg/L；
4) water planting culture: filling it up with mill water culture nutrient solution in cuttage vessel, by the suction of 2cm thickness of cuttings obtained in step 3
Water sponge strip package, and through cuttage fixed plate fixed placement in cuttage vessel；
5) manage after cuttage: water planting room temperature is 18-28 DEG C, humidity 80-100%, and nutrient solution is replaced in timing, cultivates 1-4
Week；
6) acclimatization and transplants: when the clematis cuttings root long of water planting reaches 1-2cm, which is moved into seedling-growing container, is moved
1 water is poured after cultivation daily, see after 3 days it is dry see pour, avoid direct sunlight, obtain seedling.
Nutrient is in the mill water culture nutrient solution with densimeter, N:1.25-2.0 mM/L, P:0.15-0.2 mM/L, K:
0.4-0.8 mM/L, Ca:0.4-1.0 mM/L, Mg:0.4-1.0 mM/L, Fe:10-15 mM/L, Mn:3-6 mM/L, Mo:
0.1-0.35 mM/L, B:10-18 mM/L, Zn:0.3-0.7 mM/L, Cu:0.15-0.3 mM/L, inorganic chelator EDTA-
The concentration of Na2 is 20-40 mM/L, and carries out pH value adjusting by the way that HCl solution is added.
The cuttage vessel is the plastic box of width 20-30cm, long 30-40cm, depth 10-15cm, the cuttage fixed plate
For the PVC foam plate of wide 20-30cm, long 30-40cm, thickness 0.8-1.5cm, size just covers cuttage vessel, the cuttage
Fixed plate is equipped with the circular hole that diameter is 2.5cm, is used as cuttings and places mouth.
The cuttage fixed plate is carried out disinfection with 30%NaClO, is cleaned after impregnating 20min.
Replacement in mill water culture nutrient solution every 3 days is primary, and keeps mill water culture nutrient solution to contact with water-absorbing sponge item, while avoiding sunlight
Direct projection keeps room ventilation good, and water planting is until take root.
The seedling cultivation depth is advisable with covering new root just, seedling medium for peat is uniformly mixed with perlite and
At mixed proportion 1:1.
The beneficial effects of the present invention are: using above scheme, 1, the present invention on the basis of water culture technique, it is raw to pass through plant
Influence of the long regulator to clematis cuttage root-taking improves cutting plantation；Make moisture, temperature, light during cutting propagation
According to controllable with the conditions such as air humidity, influence of the external environment to plant propagation rate is reduced, cutting plantation is stablized, and plant is raw
Long status is good；2, the present invention can expand breeding area, while overcome the problems, such as that cuttage is subject to seasonal restrictions, and shorten breeding cycle,
Cuttage survival rate is improved, cuttings survival rate is conducive to the factorial production and popularization and application of clematis plant up to 85% or more, has
There are preferable economic benefit, social benefit and ecological benefits.
Specific embodiment
Embodiment one
A kind of clematis Water culture rooting method, including following operating procedure:
1) preparation of cuttage vessel: opaque cabinet is selected to fix as cuttage vessel and PVC foam plate as cuttage
Plate, the cuttage fixed plate are equipped with the circular hole that diameter is 2.5cm, are used as cuttings and place mouth；
2) preparation of mill water culture nutrient solution: needed in the mill water culture nutrient solution comprising nutrient N, P, K, Ca, Mg, Fe, Zn,
Mn, Cu, Mo, B and inorganic chelator EDTA-Na2, and pH value is adjusted to 5.0-5.5 by the way that HCl solution is added；
Wherein, nutrient by densimeter N:1.25 mM/L, P:0.2 mM/L, K:0.5 mM/L, Ca:0.5 mM/L,
Mg:0.5 mM/L, Fe:10mM/L, Mn:4mM/L, Mo:0.2mM/L, B:10mM/L, Zn:0.3 mM/L, Cu:0.1mM/L, nothing
The concentration of machine chelating agent EDTA-Na2 is 20mM/L；
3) selection and processing of clematis cuttings: the maturation zone bud branch of the no disease and pests harm of selection semi-lignified or lignifying
As cuttings, the long 3-4cm of cuttings, top section is cross section, and lower part section is scarf, 1 blade is only stayed, by cuttings base portion
1-2cm submerges in the naphthalene acid solution that concentration is 100mg/L and impregnates 15min, then takes out and dries in the shade to ensure to absorb；
4) water planting culture: filling it up with mill water culture nutrient solution in cuttage vessel, by the suction of 2cm thickness of cuttings obtained in step 3
Water sponge strip package, and be fixed by the circular hole of cuttage fixed plate, it is immersed in cuttings in mill water culture nutrient solution, water-absorbing sponge
Then it is kept in contact with mill water culture nutrient solution；
5) manage after cuttage: water planting room temperature is 18-28 DEG C, humidity 80-100%, the water planting nutrition of replacement in every 3 days
Liquid, while direct sunlight is avoided, it keeps room ventilation good, cultivates 1-4 weeks；
6) acclimatization and transplants: when the clematis cuttings root long of water planting reaches 1-2cm, which is moved into seedling-growing container, is moved
1 water is poured after cultivation daily, see after 3 days it is dry see pour, avoid direct sunlight, obtain seedling.
Embodiment two
A kind of clematis Water culture rooting method, operating procedure are the same as example 1 substantially, the difference is that: step
Cuttings base portion 1-2cm is submerged in the naphthalene acid solution that concentration is 200mg/L in rapid 3 and impregnates 15min.
Embodiment three
A kind of clematis Water culture rooting method, operating procedure are the same as example 1 substantially, the difference is that: step
Cuttings base portion 1-2cm is submerged in the naphthalene acid solution that concentration is 300mg/L in rapid 3 and impregnates 15min.
Example IV
A kind of clematis Water culture rooting method, operating procedure are the same as example 1 substantially, the difference is that: step
Cuttings base portion 1-2cm is submerged in the indolebutyric acid solution that concentration is 100mg/L in rapid 3 and impregnates 15min.
Embodiment five
A kind of clematis Water culture rooting method, operating procedure are the same as example 1 substantially, the difference is that: step
Cuttings base portion 1-2cm is submerged in the indolebutyric acid solution that concentration is 200mg/L in rapid 3 and impregnates 15min.
Embodiment six
A kind of clematis Water culture rooting method, operating procedure are the same as example 1 substantially, the difference is that: step
Cuttings base portion 1-2cm is submerged in the indolebutyric acid solution that concentration is 300mg/L in rapid 3 and impregnates 15min.
It carries out Water culture to clematis by six kinds of different implementation methods to take root, and Statistical Comparison is as a result, obtain as follows
Conclusion:
Best with 200mg/L naphthalene acid solution and the effect of 100mg/L indolebutyric acid solution, through counting, cuttage is raw
Root success rate is respectively 89.67% and 86.43%, is above embodiment, and quantity of taking root is 2-3 times of other groups, average root long
1.65cm, and by reaching root system stable state after hardening 5 days, reach seedling cultivation and requires.
When seedling is cultivated, seedling cultivation depth is advisable with covering new root just, and seedling medium is that peat and perlite are uniform
It mixes, mixed proportion 1:1.
In addition, each the concentrating of nutrients allows there are certain error, the mill water culture nutrient solution when preparing mill water culture nutrient solution
Middle nutrient, inorganic chelator EDTA-Na2 are maintained in following range with densimeter: N:1.25-2.0 mM/L, P:
0.15-0.2 mM/L, K:0.4-0.8 mM/L, Ca:0.4-1.0 mM/L, Mg:0.4-1.0 mM/L, Fe:10-15 mM/L,
Mn:3-6 mM/L, Mo:0.1-0.35 mM/L, B:10-18 mM/L, Zn:0.3-0.7 mM/L, Cu:0.15-0.3 mM/L, nothing
Machine chelating agent EDTA-Na2:20-40 mM/L.
It is all right that the size of the cuttage vessel is just suitable for cuttings, should not be too large wasting space, also unsuitable too small
Growth is influenced, cuttage vessel generally selects the plastic box of width 20-30cm, long 30-40cm, depth 10-15cm, and the cuttage is solid
Fixed board size just covers cuttage vessel, width 20-30cm, long 30-40cm, thickness 0.8-1.5cm, the cuttage fixed plate
Before the use, 20min can be impregnated by 30%NaClO, carry out disinfection cleaning, it is ensured that nontoxic sterile to clematis cuttings one
Good growing environment, be further ensured that survival rooting rate.
Embodiment is not construed as the limitation to invention, but any based on spiritual improvements introduced of the invention, all Ying Ben
Within the protection scope of invention.
Claims (5)
1. a kind of clematis Water culture rooting method, it is characterised in that: including following operating procedure:
1) preparation of cuttage vessel: select opaque cabinet as cuttage vessel；
2) preparation of mill water culture nutrient solution: needed in the mill water culture nutrient solution comprising nutrient N, P, K, Ca, Mg, Fe, Zn, Mn, Cu,
Mo, B and inorganic chelator EDTA-Na2, and pH value is 5.0-5.5；
3) selection and processing of clematis cuttings: select the maturation zone bud branch of the no disease and pests harm of semi-lignified or lignifying as
Cuttings, the long 3-4cm of cuttings, top section are cross section, and lower part section is scarf, 1 blade are only stayed, by cuttings base portion 1-
2cm submerges and impregnates 15min in plant growth regulator, and processing of drying in the shade is taken out after immersion, and the plant growth regulator selection is dense
The indolebutyric acid (IBA) that the methyl α-naphthyl acetate (NAA) or concentration that degree is 200mg/L are 100mg/L；
4) water planting culture: filling it up with mill water culture nutrient solution in cuttage vessel, by the water suction sea of the 2cm thickness of cuttings obtained in step 3
Continuous item package, and through cuttage fixed plate fixed placement in cuttage vessel；
5) manage after cuttage: water planting room temperature is 18-28 DEG C, humidity 80-100%, and nutrient solution is replaced in timing, is cultivated 1-4 weeks；
6) acclimatization and transplants: when the clematis cuttings root long of water planting reaches 1-2cm, which is moved into seedling-growing container, after transplanting
Pour 1 water daily, see after 3 days it is dry see pour, avoid direct sunlight, obtain seedling；
Nutrient is in the mill water culture nutrient solution with densimeter, N:1.25-2.0 mM/L, P:0.15-0.2 mM/L, K:0.4-
0.8 mM/L, Ca:0.4-1.0 mM/L, Mg:0.4-1.0 mM/L, Fe:10-15 mM/L, Mn:3-6 mM/L, Mo:0.1-
0.35 mM/L, B:10-18 mM/L, Zn:0.3-0.7 mM/L, Cu:0.15-0.3 mM/L, inorganic chelator EDTA-Na2's
Concentration is 20-40 mM/L, and carries out pH value adjusting by the way that HCl solution is added.
2. clematis Water culture rooting method according to claim 1, it is characterised in that: the cuttage vessel is width
The plastic box of 20-30cm, long 30-40cm, depth 10-15cm, the cuttage fixed plate are width 20-30cm, long 30-40cm, thickness
The PVC foam plate of 0.8-1.5cm, size just cover cuttage vessel, and it is 2.5cm's that the cuttage fixed plate, which is equipped with diameter,
Circular hole is used as cuttings and places mouth.
3. clematis Water culture rooting method according to claim 1, it is characterised in that: the cuttage fixed plate is used
30%NaClO carries out disinfection, and cleans after impregnating 20min.
4. clematis Water culture rooting method according to claim 1, it is characterised in that: every 3 days of mill water culture nutrient solution is more
It changes once, and keeps mill water culture nutrient solution to contact with water-absorbing sponge item, while avoiding direct sunlight, keep room ventilation good, water
Training is until take root.
5. clematis Water culture rooting method according to claim 1, it is characterised in that: the seedling cultivation depth with
Just covering new root is advisable, and seedling medium is that peat is uniformly mixed with perlite, mixed proportion 1:1.
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| CN108834860A (en) * | 2018-07-09 | 2018-11-20 | 中国科学院昆明植物研究所 | Rare and endangered medicinal plant swordleaf dragon tree water planting Rapid Rooting seedling establishment method |
| CN109716940A (en) * | 2019-01-25 | 2019-05-07 | 福建农林大学 | A kind of method of indoor quickly breeding vine tea seedling |
| CN111133903B (en) * | 2020-01-16 | 2022-01-28 | 青岛农业大学 | Alfalfa water culture cutting propagation method |
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| CN104082152A (en) * | 2014-08-04 | 2014-10-08 | 云南农业大学 | Tissue culture and rapid propagation method for clematis ranunculoides |
| CN104396750A (en) * | 2014-11-18 | 2015-03-11 | 罗翼 | Tissue culture propagation method for clematis lanuginosa |
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| CN106818443A (en) * | 2017-03-24 | 2017-06-13 | 卢鹏 | A kind of red maple bough water planting cuttage breeding method |
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2017
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Patent Citations (5)
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| CN1868263A (en) * | 2006-06-29 | 2006-11-29 | 南京林业大学 | Tissue culture method for Clematis Multi Blue |
| CN104082152A (en) * | 2014-08-04 | 2014-10-08 | 云南农业大学 | Tissue culture and rapid propagation method for clematis ranunculoides |
| CN104396750A (en) * | 2014-11-18 | 2015-03-11 | 罗翼 | Tissue culture propagation method for clematis lanuginosa |
| CN105145176A (en) * | 2015-09-01 | 2015-12-16 | 张莘蔓 | Method for water culture cutting propagation of callistemon cirtinus |
| CN106818443A (en) * | 2017-03-24 | 2017-06-13 | 卢鹏 | A kind of red maple bough water planting cuttage breeding method |
Non-Patent Citations (1)
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