CN108102958A - One plant weight salt-soda soil plant rhizosphere growth-promoting A Shi bacillus and its application - Google Patents

One plant weight salt-soda soil plant rhizosphere growth-promoting A Shi bacillus and its application Download PDF

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CN108102958A
CN108102958A CN201711396251.6A CN201711396251A CN108102958A CN 108102958 A CN108102958 A CN 108102958A CN 201711396251 A CN201711396251 A CN 201711396251A CN 108102958 A CN108102958 A CN 108102958A
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culture medium
promoting
bacillus
gpr018
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井大炜
马海林
刘方春
刘幸红
马丙尧
杜振宇
杨庆山
周健
彭琳
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Shandong Academy of Forestry
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Abstract

The invention discloses a plant weight salt-soda soil rhizosphere growth-promoting A Shi bacillus and its applications.The bacterial strain can improve salt tolerant alkali ability of the Chinese tamarisk in weight saline-alkali environment.Bacteria agent is made in the heavy saline rhizosphere growth-promoting A Shi bacillus of the present invention, is inoculated into around Chinese tamarisk root system, after the Salt Strees Condition of certain time, compared with microbial inoculum is not inoculated with, Chinese tamarisk can be promoted to grow, improve the root system and plant water content of Chinese tamarisk.Therefore, the present invention can be used as Inoculant to be applied in weight Salty hebitats, and the salinity and alkalinity resistance to improve into Chinese tamarisk provides excellent strain resource.

Description

One plant weight salt-soda soil plant rhizosphere growth-promoting A Shi bacillus and its application
Technical field
The invention belongs to agricultural microbial technology fields, and in particular to a plant weight salt-soda soil plant rhizosphere growth-promoting A Shi gemma Bacillus and its application.
Background technology
Saline Land is to restrict one of an important factor for agriculture and forestry develop.Salination seriously limits plant growth, from And it makes a big impact to crop yield.According to statistics, the whole world causes the loss up to 11,000,000,000 of irrigation district crop due to salination Dollar, and be continuously increased.At present, salinization soil accounts for 30% or so of the land gross area, and there are about hm more than 1,000,000,000 in the whole world2's Soil is in salination state.The 2nd national reconnaissance information statistics report organized according to the Ministry of Agriculture, the total face of Chinese salinized soil Product, which accounts for the whole nation, can utilize the 4.88% of land area, wherein arable land medium salting area reaches 920.9 ten thousand hm2, account for national arable land The 6.62% of area.NaHCO3With Na2CO3It is the main component of inland Soda saline and alkaline soils, the plant being grown on such soil is held It is vulnerable to Na+, high pH and the low flow of water multiple stress, soil water permeability is caused to be reduced with aeration, the osmotic pressure of the soil liquid Increase, the validity of soil nutrient reduces, and seriously limits the normal growth of plant, causes surface vegetation community diversity continuous It reduces.
Microorganism plays an important role in terms of plant salt tolerance alkali ability is improved.One plant of cloaca is applied in Yang Qing mountains et al. Enterobacteria TIA062 (CN201610330288.8) can effectively improve the salt tolerance of Chinese wax under the conditions of certain salinity；Du Chuanying etc. People using temperature screening can be cultivated in the method that surface orientation culture is combined is to In Soils of Northeastern salt tolerant bacillus into Row separation and screening, it is believed that the salt resistance ability of bending bacillus and Bacillus subtillis is preferable；Wang Hui victories et al., which are studied, to be thought, Tomato inoculation Pseudomonas aurantica after, can acid and alkali-resistance, higher antibacterial activity is shown to germ.
Plant rhizosphere growth-promoting bacterium (Plant growth-promoting rhizobacteria, PGPR) is a kind of existence In plant rhizosphere, root table, there is the beneficial bacteria for promoting or having antagonism to pathogen to plant growth.A large number of experiments show that Many PGPR strains expresseds go out apparent promotion plant growth, prevention soil-borne disease, improve the degeneration-resistant of plants against abiotic stress Property, promote geobiont repair and other effects.PGPR directly or indirectly can promote plant to give birth to by one or more of mechanism of action Long, the direct growth-promoting mechanism of PGPR includes：Generate plant hormone, molten squama, fixed nitrogen, the thermophilic iron element of production, secretion acc deaminase etc..So And most plants and plant rhizosphere growth-promoting bacterium do not have with high salt, high salinity ability of resisting, especially in heavy salinized feelings Under condition, the beneficial microbe in plant rhizosphere environment is reduced, and causes the bioactivity of plant rhizosphere to reduce, and salt tolerance declines.Sieve Choosing improves plant salt tolerance alkalescence, performance microorganism-plant combined suitable for suitable for plant rhizosphere growth-promoting endophytic bacteria under heavy salinized environment Repair has huge application potential in heavy saline restoration of the ecosystem.
The content of the invention
In view of the above-mentioned problems of the prior art, inventor is screened for heavy saline plant rhizosphere soil, from A plant weight salt-soda soil rhizosphere growth-promoting A Shi bacillus is obtained in Chinese tamarisk rhizosphere soil, Chinese tamarisk can be improved in weight saline-alkali environment Salt tolerant alkali ability.
Specifically, the present invention relates to following technical schemes：
One of the objects of the present invention is to provide a plant weight salt-soda soil rhizosphere growth-promoting A Shi bacillus (Bacillusaryabhattai) GPR018 is preserved in Chinese microorganism strain preservation conservator on January 7th, 2016 Meeting common micro-organisms center, deposit number are CGMCC No.11973.
The second object of the present invention is to provide heavy saline rhizosphere growth-promoting A Shi bacillus (Bacillusaryabhattai) cultural method of GPR018 is especially included in what is fermented on skimmed milk power culture medium Process.
The third object of the present invention is to provide heavy saline rhizosphere growth-promoting A Shi bacillus (Bacillusaryabhattai) fermentation culture medium of GPR018, the fermentation culture medium include A Shi bacillus GPR018 Thalline.
In preferred embodiment, A Shi bacillus GPR018 clump counts are 10 in fermentation culture medium3-108cfu/mL。
The fourth object of the present invention is to provide a kind of use product comprising GPR018 bacterial strains or its fermentation culture medium, bag Include microbial bacterial agent or composition forms.
In preferred embodiment, the product includes carrier, and the carrier can be solid carrier or liquid-carrier；Example As solid carrier can be selected from clay, talcum, kaolin, montmorillonite, zeolite, silica, corn flour.Bean powder, polyvinyl alcohol or poly- Ethylene glycol, trehalose etc.；Liquid-carrier can be vegetable oil, mineral oil, water etc..In preferred embodiment, the product can Think powder morphology, solution morphology, particle shape etc..Such as the powder morphology of product can be the lyophilized of bacterial strain or its fermentate Powder.
The fourth object of the present invention is to provide above-mentioned bacterial strains or its fermentation culture medium and product under weight saline-alkali environment Promote plant growth, improve plant leaf blade water content or reduce the application in plant film quality Peroxidative damage.Preferably, the plant Object is Chinese tamarisk.
In preferred embodiment, in the application, above-mentioned bacterial strains or its fermentation culture medium and product are administered to plant Around object root system.
Saline-alkali environment of the present invention, mainly NaHCO3With Na2CO3It is Soda saline and alkaline soils for main component, growth is herein Plant on class soil is easily subject to Na+, high pH and the low flow of water multiple stress, soil water permeability is caused to be reduced with aeration, The osmotic pressure of the soil liquid increases, and the validity of soil nutrient reduces, and seriously limits the normal growth of plant.
The present invention achieves following advantageous effect：
The plant weight salt-soda soil rhizosphere growth-promoting A Shi bacillus of the present invention and its application, can improve Chinese tamarisk in geavy salt alkali ring Salt tolerant alkali ability in border.Bacteria agent is made in the heavy saline rhizosphere growth-promoting A Shi bacillus of the present invention, is inoculated into Gui Around willow root system, after the Salt Strees Condition of certain time, compared with microbial inoculum is not inoculated with, Chinese tamarisk can be promoted to grow, improve Chinese tamarisk Root system, blade and plant water content.Therefore, the present invention can be used as Inoculant to be applied in weight Salty hebitats, to improve into Chinese tamarisk Salinity and alkalinity resistance provide excellent strain resource.
Description of the drawings
Fig. 1 bacterial strain GPR018 colonial morphologies of the present invention；
Fig. 2 bacterial strain GPR018 light microscopes microscopy cyclograms of the present invention；
The phylogenetic tree of Fig. 3 bacterial strain GPR018 of the present invention
Different strains surveys OD600 values under the different salt concentrations of Fig. 4 embodiments 3
Specific embodiment
With reference to embodiment, the present invention is described further, as described below, is only the preferable implementation to the present invention Example, not limits the present invention, any person skilled in the art is possibly also with the disclosure above Technology contents be changed to the equivalent embodiment changed on an equal basis.It is every without departing from the present invention program content, according to the present invention Any simple modification for being made to following embodiment of technical spirit or equivalent variations, all fall in protection scope of the present invention.
The screening separation and preservation of 1 heavy saline plant growth-promoting rhizobacteria of embodiment
Gather 6, Huanghe delta Chinese tamarisk Rhizosphere sampling sample.By gradient dilution method, isolated from Rhizosphere sampling thin Bacterium isolate.The bacterium being separated to is purified by three zoning collimation methods, and judges whether bacterial strain purifies by microscopy, to pure Bacterium after change is numbered, and picking single bacterium colony is inoculated in sterilizing toothpick on skimmed milk power culture medium flat plate.37 DEG C of culture 2d Afterwards, the bacterium colony for transparent circle occur is chosen, measures colony diameter d and transparent loop diameter D, and calculates D/d, selects the big progress of ratio It purifies and verifies the effect of production protease repeatedly, choose the further secondary screening of bacterial strain of function-stable.
Secondary screening uses Folin phenol methods.By 1mL original fermentation liquors add 2mL mass fractions be 0.5% casein solution in pH value 7.0th, 37 DEG C of water-bath 15min, then inactivated with the trichloroacetic acid that mass fraction is 10%, then take the centrifugation of the above-mentioned reaction solutions of 1mL Supernatant adds 1mL Folin phenol in the Na of 5mL 0.55mol/L2CO3The lower 37 DEG C of water-bath 15min of alkaline condition survey under 660nm Absorbance.To add the reaction system of inactivation enzyme solution as blank.By the bacterial strain that secondary screening obtains by Chinese tamarisk nursery and potted plant experiment, Determine one plant of bacterium for promoting Chinese tamarisk growth result best, number GPR018.
The Physiology and biochemistry and Molecular Identification of 2 heavy saline plant growth-promoting rhizobacteria GPR018 of embodiment
Colony characteristics and thalli morphology：The circular rule of bacterium colony, bacterium colony is big, surface bulge, relatively moistens, yellowish, opaque, Thalline is smaller, in rod-shaped, has gemma, colonial morphology is as shown in Figure 1, its light microscope microscopy is as shown in Figure 2.
Physiological and biochemical property：Catalase test is positive, and citrate is positive using experiment, hydrolysis starch negative, V-P Measure negative, D-Glucose negative, PEARLITOL 25C negative, trehalose negative, D-glucitol experiment the moon Property, cane sugar test is negative, glucose aerogenesis negative.
Function is qualitative and quantitative determines：1mL original fermentation liquors are added into the casein solution that 2mL mass fractions are 0.5%, in pH value 7.0th, 37 DEG C of water-bath 15min, then inactivated with the trichloroacetic acid that mass fraction is 10%, then take the centrifugation of the above-mentioned reaction solutions of 1mL Supernatant adds 1mL Folin phenol in the Na of 5mL 0.55mol/L2CO3The lower 37 DEG C of water-bath 15min of alkaline condition survey under 660nm Absorbance.To add the reaction system of inactivation enzyme solution as blank.It defines at this point in the reaction：1ug tyrosine is generated per 1min Required enzyme amount is defined as an enzyme activity (IU).By measuring, proteinase activity is 47.22 μ g/ml in zymotic fluid.
The 16S rDNA sequences of GPR018
ACATTGGGGGCATGCCTATAATGCAGTCGAGCGAACTGATTAGAAGCTTGCTTCTATGACGTTAGCGGCGGACGGGT GAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGAAGCTAATACCGGATAGGATCTTCT CCTTCATGGGAGATGATTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGTGCATTAGCTAGTTGGTGA GGTAACGGCTCACCAAGGCCACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCC AGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGAT GAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACAAGAGTAACTGCTTGTACCTTGACGGTACC TAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTG GGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAAC TGGGGAACTTGAGTGCAGAAGAGAAAAGCGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACC AGTGGCGAAGGCGGCTTTTTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCC TGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCTAACGCATTAA GCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCAT GTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACTCTAGAGATAGAGCGTTCC CCTTCGGGGGACAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAA CGAGCGCAACCCTTGATCTTAGTTGCCAGCATTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAA GGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGGGCT GCAAGACCGCGAGGTCAAGCCAATCCCATAAAACCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAG CTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACAC CACGAGAGTTTGTAACACCCGAAGTCGGTGGAGTAACCGTAAGGAGCTAGCCGCCTAAGGTGGACAGGATAATGG (SEQ ID NO:1)
Identification and analysis：Phylogenetic tree is as shown in figure 3, GPPR018 is the same as A Shi gemma bars it can be seen from phylogenetic tree Bacterium (Bacillus aryabhattai) is on same branch, and the similarity of the two is therefore preliminary by GPR018 up to 98.57% It is accredited as A Shi bacillus.
Biological deposits information：One plant weight salt-soda soil rhizosphere growth-promoting A Shi bacillus, the strain classification are named as A Shi buds Spore bacillus (Bacillus aryabhattai) GPR018, is preserved in Chinese microorganism strain preservation pipe on January 7th, 2016 Reason committee common micro-organisms center (abbreviation CGMCC, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the Chinese Academy of Sciences Institute of microbiology, postcode 100101), deposit number is CGMCC No.11973.
Growth-promoting effect under the salt resistance ability and weight saline-alkali environment of embodiment 3GPR018
1. salt resistance ability
4 kinds of A Shi bacillus are selected, are respectively that (deposit number is A Shi bacillus BPR078 (CGMCCNo.11971), (deposit number is (CGMCC No.11972), A Shi bacillus to A Shi bacillus BPR088 (for (CGMCC No.11967) and A Shi bacillus GPR018 of the invention, (deposit number is (CGMCC to deposit number to JIA2 No.11973).4 plants of production auxin bacterial strains are first seeded in LB liquid medium, 180rpm, 37 DEG C of constant-temperature table cultures are for 24 hours. After 200 μ L is taken to activate bacterium solution to being 1%, 3%, 6% respectively to sodium chloride concentration, in the fluid nutrient medium of 9% LB, Mei Geti Degree three is parallel, is placed on 180rpm, 37 DEG C of constant-temperature table cultures for 24 hours, using spectrophotometer, not to be inoculated with pair of bacteria suspension Answering culture medium, turbidimetry periodically samples for blank control, the OD600 of parallel 3 measure bacterium solution.The results are shown in Figure 4, can see Go out, when sodium chloride concentration increases to 6%, the OD600 of GPR018 is significantly greater than other three kinds of A Shi bacillus, illustrates that its is resistance to Salt ability is apparently higher than other bacterial strains.
2. the application effect under weight saline-alkali environment
The GPR018 being stored on the inclined-plane methods of three rides are activated in LB (peptone 10g；NaCl 10g；Ferment Female medicinal extract 5g；Agar 15-20g；Water 1000ml；PH 7.0-7.2, packing, 121 DEG C of sterilizing 20min) on tablet.Picking single bacterium colony Into corresponding 10ml fluid nutrient mediums, 37 DEG C, 180rpm is cultivated for 24 hours.Seed liquor is seeded to accordingly by 1% inoculum concentration It ferments in 50ml fluid nutrient mediums, 37 DEG C, 180rpm cultures 2d.
The Chinese tamarisk container seedling of cultivation is transplanted in plastic tub, after 2 months a period of times of culture, Chinese tamarisk is carried out at stress Reason.2 kinds of basic salt NaHCO3And Na2CO3In molar ratio 9:1 mixing, sets 400mmol/L concentration, to irrigate salt during Stress treatment Subject to water.GPR018 bacterium solutions are inoculated with while stress, 5ml bacterium solutions is taken to dilute 10 times and are inoculated into around Chinese tamarisk root.Not to be inoculated with Bacterium solution (pouring same amount of culture medium) is as control, each 10 plants of processing, normal management.Stress treatment lasts 40 days, then The mature leaf of same area is taken to carry out the measure of physical signs.Tape measure is respectively adopted and measures plant height, calculates height growth amount (alkali Plant height when plant height-alkaline stress starts at the end of stress)；The plant height of control measures and computational methods are consistent with Stress treatment.Using Oven drying method measures the relative water content of Chinese tamarisk blade, using the malonaldehyde of thiobarbituricacidα- colorimetric method for determining Chinese tamarisk blade (MDA) content.The results are shown in Table 1, it can be seen that after Chinese tamarisk stress 40d, inoculation GPR018 can carry leaf r elative water content High by 12.29%, mda content reduces by 22.15%, and height growth amount adds 27.0%, and GPR018 can improve Gui under salt stress The salt tolerance of willow promotes Chinese tamarisk growth.
Growth-promoting effects of the table 1GPR018 under weight saline-alkali environment
SEQUENCE LISTING
<110>Shandong Forest Science Academy
<120>One plant weight salt-soda soil plant rhizosphere growth-promoting A Shi bacillus and its application
<130>
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1461
<212> DNA
<213> Bacillus aryabhattai
<400> 1
acattggggg catgcctata atgcagtcga gcgaactgat tagaagcttg cttctatgac 60
gttagcggcg gacgggtgag taacacgtgg gcaacctgcc tgtaagactg ggataacttc 120
gggaaaccga agctaatacc ggataggatc ttctccttca tgggagatga ttgaaagatg 180
gtttcggcta tcacttacag atgggcccgc ggtgcattag ctagttggtg aggtaacggc 240
tcaccaaggc cacgatgcat agccgacctg agagggtgat cggccacact gggactgaga 300
cacggcccag actcctacgg gaggcagcag tagggaatct tccgcaatgg acgaaagtct 360
gacggagcaa cgccgcgtga gtgatgaagg ctttcgggtc gtaaaactct gttgttaggg 420
aagaacaagt acaagagtaa ctgcttgtac cttgacggta cctaaccaga aagccacggc 480
taactacgtg ccagcagccg cggtaatacg taggtggcaa gcgttatccg gaattattgg 540
gcgtaaagcg cgcgcaggcg gtttcttaag tctgatgtga aagcccacgg ctcaaccgtg 600
gagggtcatt ggaaactggg gaacttgagt gcagaagaga aaagcggaat tccacgtgta 660
gcggtgaaat gcgtagagat gtggaggaac accagtggcg aaggcggctt tttggtctgt 720
aactgacgct gaggcgcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca 780
cgccgtaaac gatgagtgct aagtgttaga gggtttccgc cctttagtgc tgcagctaac 840
gcattaagca ctccgcctgg ggagtacggt cgcaagactg aaactcaaag gaattgacgg 900
gggcccgcac aagcggtgga gcatgtggtt taattcgaag caacgcgaag aaccttacca 960
ggtcttgaca tcctctgaca actctagaga tagagcgttc cccttcgggg gacagagtga 1020
caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg 1080
agcgcaaccc ttgatcttag ttgccagcat ttagttgggc actctaaggt gactgccggt 1140
gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg acctgggcta 1200
cacacgtgct acaatggatg gtacaaaggg ctgcaagacc gcgaggtcaa gccaatccca 1260
taaaaccatt ctcagttcgg attgtaggct gcaactcgcc tacatgaagc tggaatcgct 1320
agtaatcgcg gatcagcatg ccgcggtgaa tacgttcccg ggccttgtac acaccgcccg 1380
tcacaccacg agagtttgta acacccgaag tcggtggagt aaccgtaagg agctagccgc 1440
ctaaggtgga caggataatg g 1461

Claims (10)

1. plant weight salt-soda soil rhizosphere growth-promoting A Shi bacillus (Bacillus aryabhattai) GPR018, in 2016 January 7 was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC No.11973。
2. heavy saline rhizosphere growth-promoting A Shi bacillus (Bacillus aryabhattai) GPR018 described in claim 1 Cultural method is included in the process fermented on skimmed milk power culture medium.
3. heavy saline rhizosphere growth-promoting A Shi bacillus (Bacillus aryabhattai) GPR018 described in claim 1 Fermentation culture medium, which is characterized in that the fermentation culture medium includes A Shi bacillus GPR018 thalline.
4. fermentation culture medium according to claim 3, which is characterized in that A Shi bacillus GPR018 in fermentation culture medium Clump count is 103-108cfu/mL。
5. a kind of use product, bag comprising any one of bacterial strain or claim the 3-4 fermentation culture medium described in claim 1 Include microbial bacterial agent or composition forms.
6. product according to claim 5, which is characterized in that the product includes carrier, the carrier for solid carrier or Liquid-carrier；
Preferably, solid carrier is selected from clay, talcum, kaolin, montmorillonite, zeolite, silica, corn flour, bean powder, polyvinyl alcohol With the one or more in/polyethylene glycol, trehalose；
The one kind of liquid-carrier in vegetable oil, mineral oil, water.
7. product according to claim 5, which is characterized in that the product be powder morphology, solution morphology or particle shape, Preferably, the powder morphology of product is the freeze-dried powder of bacterial strain or fermentation culture medium.
8. any one of any one of bacterial strain described in claim 1 or claim the 3-4 fermentation culture medium, claim 5-7 institute Product is stated to promote plant growth under weight saline-alkali environment, improve plant leaf blade water content or reduce in plant film quality Peroxidative damage Application.
9. application according to claim 8, which is characterized in that the plant is Chinese tamarisk.
10. application according to claim 8 or claim 9, which is characterized in that in the application, by bacterial strain or fermentation culture medium, production Product are administered to around root system of plant.
CN201711396251.6A 2017-12-21 2017-12-21 Plant rhizosphere growth promoting Bacillus aryabhattai for heavy saline-alkali soil and application thereof Active CN108102958B (en)

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CN113897200A (en) * 2021-11-27 2022-01-07 河北萌帮生物科技有限公司 Microbial soil remediation agent and preparation method thereof
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Publication number Priority date Publication date Assignee Title
CN109136153A (en) * 2018-09-29 2019-01-04 中国科学院成都生物研究所 One plant of salt tolerant bacillus for having a Plant growth promotion
CN110731223A (en) * 2019-11-15 2020-01-31 南京林业大学 Method for promoting growth of salix jinguiensis under stress of salts
CN110731223B (en) * 2019-11-15 2022-02-22 南京林业大学 Method for promoting growth of salix psammophila under salt stress
CN110982743A (en) * 2019-12-16 2020-04-10 沈阳农业大学 Bacillus aryabhattai for promoting corn root development and application thereof
CN112410244A (en) * 2020-09-30 2021-02-26 山东农业大学 Bacillus aryabhattai and application thereof
CN113897200A (en) * 2021-11-27 2022-01-07 河北萌帮生物科技有限公司 Microbial soil remediation agent and preparation method thereof
CN113897200B (en) * 2021-11-27 2024-02-02 河北萌帮生物科技有限公司 Microbial soil restoration agent and preparation method thereof
CN115851485A (en) * 2022-08-11 2023-03-28 华中农业大学 Bacillus bacteria and application thereof in improving saline-alkali resistance of plants
CN115851485B (en) * 2022-08-11 2023-08-29 华中农业大学 Bacillus bacteria and application thereof in improving salt and alkali tolerance of plants
CN115777489A (en) * 2022-11-23 2023-03-14 山东省林业科学研究院 Biological greening method for garden engineering

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