CN108102910A - A kind of nucleic acid extraction and amplification all-in-one machine - Google Patents
A kind of nucleic acid extraction and amplification all-in-one machine Download PDFInfo
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- CN108102910A CN108102910A CN201611055235.6A CN201611055235A CN108102910A CN 108102910 A CN108102910 A CN 108102910A CN 201611055235 A CN201611055235 A CN 201611055235A CN 108102910 A CN108102910 A CN 108102910A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M41/48—Automatic or computerized control
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Abstract
The invention discloses a kind of nucleic acid extraction with expanding all-in-one machine, including：First cabin；Nucleic acid-extracting apparatus is arranged in the first cabin, for extracting nucleic acid from sample；Second cabin；Reagent adding set, it is arranged in the second cabin, for adding nucleic acid amplification reaction reagent into the nucleic acid that nucleic acid-extracting apparatus extracts, reagent storage area is provided in second cabin, for storing nucleic acid amplification reaction reagent, the first closed cofferdam, the 3rd cabin are provided between first cabin and the second cabin；Nucleic acid amplifier is arranged in the 3rd cabin, for being expanded to the nucleic acid after addition nucleic acid amplification reaction reagent, wherein, the second closed cofferdam is provided between the first cabin and the 3rd cabin.Nucleic acid extraction is isolated with nucleic acid amplification using multiple cabins, meanwhile, when carrying out nucleic acid transfer, gas barrier is carried out using cofferdam, to prevent the indoor air contact in each cabin from cross contamination occurs.
Description
Technical field
The present invention relates to Nucleic acid manipulation techniques fields, and in particular to a kind of nucleic acid extraction and amplification all-in-one machine.
Background technology
Nucleic acid extraction refers to the process of complete sample nucleic acid extraction, generally use core using mating nucleic acid extracting reagent
Sour extraction apparatus carries out nucleic acid extraction.Nucleic acid amplification refers to the technology that nucleic acid amplification duplication is realized in vitro, nucleic acid amplification
Reaction realizes the amplification to micro sample of nucleic acid and detection, has been widely used in the fields such as biological medicine.At present,
Include polymerase chain reaction PCR, nucleic acid loop-mediated isothermal amplification LAMP, the amplification for relying on nucleotide sequence through having developed
The multiple nucleic acids such as the nucleic acid amplification reaction HAD that NASBA, rolling circle amplification reaction RCA, ligation amplification reaction LCR, unwindase rely on expand
Increasing method.But there is the easy cross contamination of amplified production in these nucleic acid amplification methods based on target sequence, by product
The false positive signal that pollution generates can cause the misinterpretation of testing result.Although serial of methods has been developed to prevent from expanding
The cross contamination of product, the method for such as carry out stringent zonal control to PCR operations, expanding and detecting using stopped pipe utilize enzyme
Method that solution method eliminates pollution etc., but these methods are more demanding to experiment condition and operating personnel, and human factor shadow
Sound is larger.In addition, template used in existing amplification method is all by independent sample extraction process, and in extraction process easily
Template cross contamination is caused, influences the judgement of pattern detection result.
The content of the invention
The technical problem to be solved in the present invention is in the prior art to the nucleic acid extraction process of sample and nucleic acid amplification mistake
Journey be easy to cause cross contamination, so as to provide a kind of nucleic acid extraction and amplification all-in-one machine.
The one side of the embodiment of the present invention provides a kind of nucleic acid extraction and amplification all-in-one machine, including：First cabin；Core
Sour extraction element is arranged in first cabin, for extracting nucleic acid from sample；Second cabin；Reagent adding set, if
It puts in second cabin, for adding nucleic acid amplification reaction reagent, institute into the nucleic acid that the nucleic acid-extracting apparatus extracts
State and reagent storage area be provided in the second cabin, for storing the nucleic acid amplification reaction reagent, first cabin with it is described
The first closed cofferdam is provided between second cabin, the nucleic acid of the nucleic acid-extracting apparatus extraction is defeated from first cabin
It send to first cofferdam, air cleaning in cabin is carried out in first cofferdam, is then sent to second cabin
It is interior；3rd cabin；Nucleic acid amplifier is arranged in the 3rd cabin, after to addition nucleic acid amplification reaction reagent
Nucleic acid is expanded, wherein, the second closed cofferdam is provided between first cabin and the 3rd cabin, it is described to add
The nucleic acid after nucleic acid amplification reaction reagent is added to be delivered to second cofferdam from second cabin, in second cofferdam
Air cleaning in interior carry out cabin, is then sent in the 3rd cabin, and nucleic acid amplification is carried out by the nucleic acid amplifier.
Optionally, first cofferdam and second cofferdam are respectively arranged with air inlet and gas outlet.
Optionally, further include：Second conveyer is arranged in first cabin, for the nucleic acid extraction to be filled
The nucleic acid for putting extraction is delivered to first cofferdam；Second conveyer is arranged in second cabin, for by described in
Nucleic acid in first cofferdam is delivered to second cofferdam；3rd transmission device is arranged in the 3rd cabin, is used for
Nucleic acid in second cofferdam is delivered to the 3rd cofferdam.
Optionally, further include：First manipulator, the nucleic acid for the nucleic acid-extracting apparatus to be extracted are placed into described
On one transmission device；Second manipulator, the nucleic acid for the 3rd transmission device to be delivered to the 3rd cabin are placed into
On the nucleic acid amplifier.
Optionally, first cabin includes the first sub- cabin and the second sub- cabin, the first sub- cabin and described the
Two sub- cabins are mutually isolated, wherein, the nucleic acid-extracting apparatus is arranged in the described first sub- cabin；In the second sub- cabin
Reagent adding set is provided with, for adding nucleic acid amplification reaction reagent into the nucleic acid that the nucleic acid-extracting apparatus extracts.
Optionally, waste liquid cabin is provided in the described first sub- cabin, for storing waste liquid caused by nucleic acid extraction.
Optionally, waste liquid cabin independent closed.
Optionally, the nucleic acid amplifier is PCR instrument.
Optionally, further include：Master board, for the nucleic acid-extracting apparatus, the reagent adding set and described
Nucleic acid amplifier is controlled.
Optionally, further include：Multiple interfaces, it is described for connecting exterior terminal and being communicated with the exterior terminal
Multiple interfaces include following at least two：RJ-45 interfaces, RJ-11 interface, SC optical fiber interfaces, fddi interface, AUI interfaces, BNC
Interface, Console interfaces, USB interface；Interface convertor is arranged in the master board, electric respectively with the multiple interface
Connection, for the data of the master board to be converted into the information that any of the multiple interface interface can transmit；
Wireless communication module is arranged in the master board, for realizing wireless telecommunications with exterior terminal.
In the embodiment of the present invention, nucleic acid-extracting apparatus carries out nucleic acid extraction in the first cabin, and the nucleic acid after extraction passes through
First cofferdam carries out isolation cleaning and then enters the second cabin, and reagent adding set adds nucleic acid amplification reaction reagent,
Then after carrying out isolation cleaning by the second cofferdam, the 3rd cabin is sent into, it is anti-to carry out nucleic acid amplification by accounting amplification device
Should, nucleic acid extraction is isolated with nucleic acid amplification using each cabin, meanwhile, when carrying out nucleic acid transfer, using cofferdam into
Row gas barrier, to prevent the indoor air contact in each cabin from cross contamination occurs.
Description of the drawings
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution of the prior art
Embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, in describing below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the schematic diagram for the specific example that amplifying nucleic acid of the embodiment of the present invention is extracted with expanding all-in-one machine；
Fig. 2 is the schematic diagram for another specific example that amplifying nucleic acid of the embodiment of the present invention is extracted with expanding all-in-one machine.
Specific embodiment
Technical scheme is clearly and completely described below in conjunction with attached drawing, it is clear that described implementation
Example is part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill
Personnel's all other embodiments obtained without making creative work, belong to the scope of protection of the invention.
In the description of the present invention, it is necessary to explanation, term " " center ", " on ", " under ", "left", "right", " vertical ",
The orientation or position relationship of the instructions such as " level ", " interior ", " outer " be based on orientation shown in the drawings or position relationship, merely to
Convenient for the description present invention and simplify description rather than instruction or imply signified device or element must have specific orientation,
With specific azimuth configuration and operation, therefore it is not considered as limiting the invention.In addition, term " first ", " second ",
" the 3rd " is only used for description purpose, and it is not intended that instruction or hint relative importance.
In the description of the present invention, it is necessary to illustrate, unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected or be integrally connected；It can
To be mechanical connection or be electrically connected；It can be directly connected, can also be indirectly connected by intermediary, it can be with
It is the connection inside two elements, can is wireless connection or wired connection.For those of ordinary skill in the art
For, the concrete meaning of above-mentioned term in the present invention can be understood with concrete condition.
As long as in addition, technical characteristic involved in invention described below different embodiments non-structure each other
It can be combined with each other into conflict.
The present embodiment provides a kind of nucleic acid extraction and amplification all-in-one machine, for carrying out nucleic acid extraction to sample, then to carrying
The nucleic acid got is expanded, as shown in Figure 1, the nucleic acid extraction includes with amplification all-in-one machine：First cabin 10, nucleic acid extraction dress
Put the 20, second cabin 30, reagent adding set 100, the 3rd cabin 130 and nucleic acid amplifier 40.
Nucleic acid-extracting apparatus 20 is arranged in the first cabin 10, for extracting nucleic acid from sample.Nucleic acid-extracting apparatus 20
Instrument for extracting nucleic acid is properly termed as, for the reagent for using adaptation automatically to be come out the nucleic acid extraction in sample.
Reagent adding set 100 is arranged in the second cabin 30, for adding into the nucleic acid that nucleic acid-extracting apparatus 20 extracts
Add nucleic acid amplification reaction reagent, reagent storage area (not shown) is provided in the second cabin 30, for storing nucleic acid amplification
Reaction reagent, is provided with the first closed cofferdam 140 between the first cabin 10 and the second cabin 30, nucleic acid-extracting apparatus 20 carries
The nucleic acid taken is delivered to the first cofferdam 140 from the first cabin 10, carries out air cleaning in cabin in the first cofferdam 140, so
After be delivered in the second cabin 30.Optionally, in order to ensure the activity of reagent, it is Celsius that the temperature of reagent storage area is maintained at 2-8
Degree.Area in preparation (not shown) is also provided in second cabin 30, after the region is for placing added complete reagent
Sample of nucleic acid.
Nucleic acid amplifier 40 is arranged in the 3rd cabin 130, for the nucleic acid after addition nucleic acid amplification reaction reagent
It is expanded, wherein, the second closed cofferdam 50 is provided between the second cabin 30 and the 3rd cabin 130, addition nucleic acid expands
Increase the nucleic acid after reaction reagent and be delivered to the second cofferdam 50 from the second cabin 30, air in cabin is carried out in the second cofferdam 50
Cleaning, is then sent in the 3rd cabin 130, nucleic acid amplification is carried out by nucleic acid amplifier 40.
In the embodiment of the present invention, nucleic acid-extracting apparatus carries out nucleic acid extraction in the first cabin, and the nucleic acid after extraction passes through
First cofferdam carries out isolation cleaning and then enters the second cabin, and reagent adding set adds nucleic acid amplification reaction reagent,
Then after carrying out isolation cleaning by the second cofferdam, the 3rd cabin is sent into, it is anti-to carry out nucleic acid amplification by accounting amplification device
Should, nucleic acid extraction is isolated with nucleic acid amplification using each cabin, meanwhile, when carrying out nucleic acid transfer, using cofferdam into
Row gas barrier, to prevent the indoor air contact in each cabin from cross contamination occurs.
Air cleaning in cofferdam may be employed inner air self-loopa filtering and realize cleaning, can also be noted by outside
Enter clean air into line replacement, as shown in Fig. 2, being provided with air inlet 51 and gas outlet 52 in the second cofferdam 50.Specifically, carry
After getting nucleic acid, hatch door 53 is opened, and the nucleic acid extracted is transported in the second cofferdam 50, and hatch door 53 is closed, second every
Confined space is formed from cabin 50, air inlet 51 and gas outlet 52 are opened, and clean air are injected from air inlet 51, by the second cofferdam
Air in 50 is discharged from gas outlet 52.It is opened by the processing of the second cofferdam and then by hatch door 54, in order to extract
Nucleic acid be delivered to reagent adding set 100 carry out reagent addition.Wherein, the set-up mode of the first isolation cabin is similarly, no longer superfluous
It states.
As shown in Fig. 2, the all-in-one machine of the embodiment of the present invention further includes：Second conveyer 60 is arranged on the first cabin 10
Interior, the nucleic acid for nucleic acid-extracting apparatus 20 to be extracted is delivered to the second cofferdam 50；Second conveyer 150 is arranged on
In two cabins 30, for the nucleic acid in the second cofferdam 50 to be delivered to the second cofferdam 50；3rd transmission device 70, is arranged on
In 3rd cabin 130, for the nucleic acid in the second cofferdam 50 to be delivered to the 3rd cofferdam 130.
Further, which further includes：First manipulator 80, the nucleic acid for nucleic acid-extracting apparatus 20 to be extracted are put
It puts in second conveyer 60；Second manipulator 90, for the 3rd transmission device 70 to be delivered to the nucleic acid in the 3rd cabin 130
It is placed on nucleic acid amplifier 40.
According to embodiments of the present invention, by using manipulator and transmission device, expand so as to fulfill nucleic acid extraction and nucleic acid
The automation of increasing is started to participate in without staff.
Still optionally further, in order to avoid pollution of the waste liquid to the first sub- cabin caused by during nucleic acid extraction, because
This is provided with waste liquid cabin 110 in the first sub- cabin, for storing waste liquid caused by nucleic acid extraction.The waste liquid cabin 110 is independent
It is closed.
In the embodiment of the present invention, nucleic acid amplifier 40 is PCR instrument.The PCR instrument includes but not limited to qualitative PCR instrument, glimmering
Light pcr instrument, regular-PCR instrument.Wherein, fluorescent PCR instrument can be isothermal fluorescent PCR instrument or the instrument of identical functions.
Optionally, further include：Master board, for nucleic acid-extracting apparatus, reagent adding set and nucleic acid amplifier
It is controlled.
Optionally, further include：Multiple interfaces, for connecting exterior terminal and being communicated with exterior terminal, multiple interfaces
Including following at least two：RJ-45 interfaces, RJ-11 interface, SC optical fiber interfaces, fddi interface, AUI interfaces, bnc interface,
Console interfaces, USB interface；Interface convertor is arranged in master board, is electrically connected with multiple interfaces, for that will lead
The data of control panel are converted into the information that any of multiple interfaces interface can transmit；Wireless communication module is arranged on master
On control panel, for realizing wireless telecommunications with exterior terminal.
In the embodiment of the present invention, multiple interfaces are integrated on all-in-one machine, can there is multiple and different species, the interface of signal,
To be suitable for various exterior terminal interfaces, in this way, staff can be connected using various types of terminals with all-in-one machine, without
Use dedicated computer, you can it is monitored, also, by adding wireless communication module, can realize wireless prison
Control, as long as exterior terminal establishes wireless connection with PCR instrument, staff can be anywhere being realized to nucleic acid extraction and expansion
Increase the monitoring of detection process, greatly improve convenience and the intelligence of all-in-one machine.
Optionally, the wireless communication module of the embodiment of the present invention includes at least one of：WIFI module, bluetooth module,
Zigbee module, mobile communication module.For wireless monitor closely, exterior terminal can pass through bluetooth mould with all-in-one machine
Block, Zigbee module, WIFI module establish connection, realize wireless telecommunications and data monitoring, for remote monitoring, then can use
Mobile communication module is established long distance wireless with exterior terminal and is connected, facilitate staff's remote monitoring all-in-one device reaction process and
As a result.
Further, mobile communication module includes：Gsm module, GPRS module, 3G module, 4G modules.
In the embodiment of the present invention, exterior terminal is one of the following：Router, desktop computer, mobile phone, tablet computer, notes
This computer.Wherein, when exterior terminal is router, it that is to say and the reaction process data and result of all-in-one machine are uploaded to network
Server, staff can extract these data on network server.In the embodiment of the present invention, user can use individual
The terminals such as mobile phone, computer and all-in-one machine instrument, which are established, to be connected, so as to the monitoring to showing anywhere in fact.
Obviously, the above embodiments are merely examples for clarifying the description, and is not intended to limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And the obvious variation thus extended out or
Among changing still in the protection domain of the application.
Claims (10)
1. a kind of nucleic acid extraction and amplification all-in-one machine, which is characterized in that including：
First cabin；
Nucleic acid-extracting apparatus is arranged in first cabin, for extracting nucleic acid from sample；
Second cabin；
Reagent adding set is arranged in second cabin, for being added into the nucleic acid that the nucleic acid-extracting apparatus extracts
Nucleic acid amplification reaction reagent is provided with reagent storage area in second cabin, for storing the nucleic acid amplification reaction reagent,
The first closed cofferdam, the core of the nucleic acid-extracting apparatus extraction are provided between first cabin and second cabin
Acid is delivered to first cofferdam from first cabin, air cleaning in cabin is carried out in first cofferdam, then
It is delivered in second cabin；
3rd cabin；
Nucleic acid amplifier is arranged in the 3rd cabin, for being carried out to the nucleic acid after addition nucleic acid amplification reaction reagent
Amplification, wherein, the second closed cofferdam is provided between first cabin and the 3rd cabin, the addition nucleic acid expands
Increase the nucleic acid after reaction reagent and be delivered to second cofferdam from second cabin, cabin is carried out in second cofferdam
Interior air cleaning is then sent in the 3rd cabin, and nucleic acid amplification is carried out by the nucleic acid amplifier.
2. nucleic acid extraction according to claim 1 and amplification all-in-one machine, which is characterized in that first cofferdam and described
Second cofferdam is respectively arranged with air inlet and gas outlet.
3. nucleic acid extraction according to claim 1 and amplification all-in-one machine, which is characterized in that further include：
Second conveyer is arranged in first cabin, and the nucleic acid for the nucleic acid-extracting apparatus to be extracted is delivered to
First cofferdam；
Second conveyer is arranged in second cabin, described for the nucleic acid in first cofferdam to be delivered to
Second cofferdam；
3rd transmission device is arranged in the 3rd cabin, described for the nucleic acid in second cofferdam to be delivered to
3rd cofferdam.
4. nucleic acid extraction according to claim 3 and amplification all-in-one machine, which is characterized in that further include：
First manipulator, the nucleic acid for the nucleic acid-extracting apparatus to be extracted are placed into the second conveyer；
Second manipulator, the nucleic acid for the 3rd transmission device to be delivered to the 3rd cabin are placed into the nucleic acid and expand
It is installed in addition with and puts.
5. nucleic acid extraction according to claim 1 and amplification all-in-one machine, which is characterized in that be provided in first cabin
Waste liquid cabin, for storing waste liquid caused by nucleic acid extraction.
6. nucleic acid extraction according to claim 5 and amplification all-in-one machine, which is characterized in that waste liquid cabin independent closed.
7. according to claim 1-6 any one of them nucleic acid extraction and amplification all-in-one machine, which is characterized in that the nucleic acid amplification
Device is PCR instrument.
8. nucleic acid extraction according to claim 7 and amplification all-in-one machine, which is characterized in that the PCR instrument is fluorescent PCR
Instrument.
9. according to claim 1-6 any one of them nucleic acid extraction and amplification all-in-one machine, which is characterized in that further include：
Master board, for being controlled to the nucleic acid-extracting apparatus, the reagent adding set and the nucleic acid amplifier
System.
10. nucleic acid extraction according to claim 9 and amplification all-in-one machine, which is characterized in that further include：
Multiple interfaces, for connecting exterior terminal and being communicated with the exterior terminal, the multiple interface include with down toward
It is two few：RJ-45 interfaces, RJ-11 interface, SC optical fiber interfaces, fddi interface, AUI interfaces, bnc interface, Consol e interfaces,
USB interface；
Interface convertor is arranged in the master board, is electrically connected with the multiple interface, for by the main control
The data of plate are converted into the information that any of the multiple interface interface can transmit；
Wireless communication module is arranged in the master board, for realizing wireless telecommunications with exterior terminal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611055235.6A CN108102910A (en) | 2016-11-25 | 2016-11-25 | A kind of nucleic acid extraction and amplification all-in-one machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611055235.6A CN108102910A (en) | 2016-11-25 | 2016-11-25 | A kind of nucleic acid extraction and amplification all-in-one machine |
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| CN108102910A true CN108102910A (en) | 2018-06-01 |
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ID=62204357
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| Application Number | Title | Priority Date | Filing Date |
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| CN201611055235.6A Pending CN108102910A (en) | 2016-11-25 | 2016-11-25 | A kind of nucleic acid extraction and amplification all-in-one machine |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642046A (en) * | 2018-05-16 | 2018-10-12 | 上海科源电子科技有限公司 | Integral biological sample treatment and detection device and its method |
| CN109337807A (en) * | 2018-09-30 | 2019-02-15 | 苏州百源基因技术有限公司 | PCR assembly line |
| CN109825416A (en) * | 2018-11-16 | 2019-05-31 | 苏州新波生物技术有限公司 | A kind of fully automatic molecular diagnostic system and molecular diagnosis method |
| WO2020057193A1 (en) * | 2018-09-19 | 2020-03-26 | 基蛋生物科技股份有限公司 | Fluorescence detection system for nucleic acid extraction and amplification |
| CN111575174A (en) * | 2020-05-11 | 2020-08-25 | 中国医学科学院北京协和医院 | Nucleic acid extraction and amplification all-in-one |
| CN113970473A (en) * | 2021-08-31 | 2022-01-25 | 河南工业大学 | PCR experiment operation panel |
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| US20130230860A1 (en) * | 2010-10-27 | 2013-09-05 | Bioneer Corporation | Automatic real-time pcr system for the various analysis of biological sample |
| US20130284028A1 (en) * | 2011-05-10 | 2013-10-31 | Zhejiang Vacin Bio-Pharmaceutical Ltd. | Apparatus for Dust Removal by Air Curtain Isolation and Self-Circulation Purification |
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| CN103555558A (en) * | 2010-07-23 | 2014-02-05 | 贝克曼考尔特公司 | Vessel for real time pcr |
| US20130230860A1 (en) * | 2010-10-27 | 2013-09-05 | Bioneer Corporation | Automatic real-time pcr system for the various analysis of biological sample |
| US20130284028A1 (en) * | 2011-05-10 | 2013-10-31 | Zhejiang Vacin Bio-Pharmaceutical Ltd. | Apparatus for Dust Removal by Air Curtain Isolation and Self-Circulation Purification |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642046A (en) * | 2018-05-16 | 2018-10-12 | 上海科源电子科技有限公司 | Integral biological sample treatment and detection device and its method |
| WO2020057193A1 (en) * | 2018-09-19 | 2020-03-26 | 基蛋生物科技股份有限公司 | Fluorescence detection system for nucleic acid extraction and amplification |
| CN109337807A (en) * | 2018-09-30 | 2019-02-15 | 苏州百源基因技术有限公司 | PCR assembly line |
| WO2020062315A1 (en) * | 2018-09-30 | 2020-04-02 | 苏州百源基因技术有限公司 | Pcr pipeline |
| CN109825416A (en) * | 2018-11-16 | 2019-05-31 | 苏州新波生物技术有限公司 | A kind of fully automatic molecular diagnostic system and molecular diagnosis method |
| CN111575174A (en) * | 2020-05-11 | 2020-08-25 | 中国医学科学院北京协和医院 | Nucleic acid extraction and amplification all-in-one |
| CN113970473A (en) * | 2021-08-31 | 2022-01-25 | 河南工业大学 | PCR experiment operation panel |
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Application publication date: 20180601 |