CN108101707B - Total nutrient microbial amino acid water-soluble fertilizer and preparation method thereof - Google Patents
Total nutrient microbial amino acid water-soluble fertilizer and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a full-nutrient microbial amino acid water-soluble fertilizer and a preparation method thereof, wherein the amino acid water-soluble fertilizer comprises mixed fermentation liquor and amino acid powder, the mixed fermentation liquor consists of silicate bacteria fermentation liquor and pediococcus pentosaceus fermentation liquor, the effective bacteria content of the silicate bacteria fermentation liquor is more than 100 hundred million/g, the effective bacteria content of the pediococcus pentosaceus fermentation liquor is more than 50 hundred million/g, the weight ratio of the silicate bacteria fermentation liquor to the pediococcus pentosaceus fermentation liquor is (1-3) to (1-3), the weight of the amino acid powder is 1-5 times of that of the mixed fermentation liquor, in the amino acid powder, glycine accounts for more than or equal to 23 percent of the weight of the amino acid powder, and arginine accounts for less than or equal to. The total nutrient microbial amino acid water-soluble fertilizer realizes promotion of crop stage production, increase of yield and reduction of diseases.
Description
Technical Field
The invention belongs to the field of fertilizers, and particularly relates to a total-nutrient microbial amino acid water-soluble fertilizer and a preparation method thereof.
Background
Amino acids are essential nutrients for plant growth and are basic materials for constructing cells and repairing tissues. Because of the characteristics of amino acid, the amino acid is used as an accelerant and a catalyst for synthesizing various enzymes in plants, plays an important role in plant metabolism, can increase the chlorophyll content of the plants, improve the activity of the enzymes, promote the permeation of carbon dioxide, enable the photosynthesis to be more vigorous, and plays an important role in improving the quality of crops and increasing the content of Vc and sugar.
Researches show that the crops have high absorption, transportation and utilization capacity for amino acid water-soluble fertilizers, can quickly supplement essential nutrient elements and amino acids, effectively regulate nutrient absorption and nutrient accumulation, promote robust growth of plants and improve yield, quality and stress resistance of agricultural products.
Metabolites generated by microorganisms in the growth process play important roles in repairing and improving soil environment, promoting the growth of crop roots, reducing the harm of soil-borne diseases, reducing the usage amount of chemical pesticides and the like. With the increasing importance of food safety, the research and development of microorganism-related products with high efficiency, low toxicity and strong functionality are vigorously developed, the effect of preventing diseases by using bacteria is achieved, the input and use of chemical pesticides in production are reduced, the sustainable development of improving the quality safety of agricultural products and protecting the agricultural ecological environment is realized, the agricultural production cost is reduced, and the cost saving and the efficiency increasing of farmers are promoted.
At present, a great amount of unreasonable chemical fertilizers are used in agriculture in China, so that increasingly serious modern agricultural diseases such as soil fertility reduction, plant diseases and insect pests increase, agricultural product quality reduction and the like are caused, and water resources are extremely deficient, so that the state greatly improves advocate 'water and fertilizer integration', improves a fertilization mode and develops water-saving agriculture. The amino acid water-soluble fertilizer as a novel fertilizer with a remarkable water-saving function is valued and gradually popularized, and has been accepted by more and more soil fertilizer specialists, agricultural technology popularization experts and farmers.
However, most of the amino acid water-soluble fertilizers sold in the current market implement the lowest standard of the Ministry of agriculture NY1429-2010, the content of trace elements is more than 20g/L or the content of the trace elements is more than 30g/L, the large, medium and trace elements are not added in a complete or reasonable proportion, the amino acid water-soluble fertilizers containing beneficial microorganisms are few and few, and crops are required to be fertilized for multiple times to supplement other nutrient substances, so that the mutual promotion and absorption balance among the elements are difficult to achieve, the fertilization cost is increased, and the water conservation is also in void.
According to the problems, silicate bacteria fermentation liquor and pediococcus pentosaceus fermentation liquor are used as the basis, the compound amino acid powder is dissolved, compound microorganism amino acid-containing solution is prepared, and macroelements and amino acid chelated medium and trace elements are added in a reasonable proportion.
Silicate bacteria, also called bacillus mucilaginosus, have the important characteristics of decomposing potassium and silicon in minerals such as feldspar, mica and the like, decomposing phosphorus in apatite, and secreting plant growth stimulin and various enzymes to enhance the resistance of crops to certain diseases. The potassium in the bacillus mucilaginosus is dissociated after the thalli die, and can be absorbed and utilized by plants. As an important functional bacterium in microbial fertilizer, the fertilizer can improve the content of quick-acting potassium and phosphorus in soil, improve the yield and quality of crops and the like.
Pediococcus pentosaceus belongs to the genus Pediococcus, is widely used in the production of various traditional fermented foods and beverages, and is commonly used in the research of fermented soymilk and the like. Although useful in the agricultural field, it is often antagonistic when used in combination with other bacteria, either to the growth of the other bacteria or to the bacteria by which it is antagonistic.
The invention aims to solve the technical problem of realizing the mutual combination and promotion of silicate bacteria and pediococcus pentosaceus in crop fertilizers. In particular to a full-nutrient microbial amino acid water-soluble fertilizer which can effectively promote the growth and development of crops, enhance the stress resistance of the crops, increase the yield of the crops and improve the soil structure.
Disclosure of Invention
In order to ensure that silicate bacteria can decompose ores in soil and residual organic matters in soil, silicate bacteria fermentation liquor and pediococcus pentosaceus fermentation liquor are adopted, but because the two fermentation liquors can generate partial antagonism in the decomposition process, namely, the two fermentation liquors have mutually inhibited production in soil, amino acid needs to be added, the initial amount of the amino acid ensures the propagation of the bacteria, and the rapid fermentation of the two bacteria is realized after the initial propagation.
In order to solve the technical problems, the invention provides a full-nutrition type amino acid water-soluble fertilizer which comprises mixed fermentation liquor and amino acid powder, wherein the mixed fermentation liquor consists of silicate bacteria fermentation liquor and pediococcus pentosaceus fermentation liquor, the effective bacteria content of the silicate bacteria fermentation liquor is more than 100 hundred million/g, the effective bacteria content of the pediococcus pentosaceus fermentation liquor is more than 50 hundred million/g, the weight ratio of the silicate bacteria fermentation liquor to the pediococcus pentosaceus fermentation liquor is (1-3) to (1-3), the weight of the amino acid powder is 1-5 times of the weight of the mixed fermentation liquor, in the amino acid powder, glycine accounts for more than or equal to 23 percent of the weight of the amino acid powder, and arginine accounts for less than or equal to 8 percent of the weight of the amino acid powder.
Preferably, in the full-nutrient amino acid water-soluble fertilizer, glycine accounts for 23-50% of the weight of the amino acid powder, and arginine accounts for 1-8% of the weight of the amino acid powder.
Preferably, the full-nutrient amino acid water-soluble fertilizer also comprises a macroelement fertilizer, a secondary element fertilizer and a trace element fertilizer, wherein the weight of the macroelement fertilizer is 1-6 times of the weight of the mixed fermentation broth, the weight of the secondary element fertilizer is 0.3-3 times of the weight of the mixed fermentation broth, and the weight of the trace element fertilizer is 0.1-1 time of the weight of the mixed fermentation broth.
Preferably, in the full-nutrient amino acid water-soluble fertilizer, the macroelement fertilizer consists of urea, potassium dihydrogen phosphate and potassium nitrate, and the weight ratio of the urea to the potassium dihydrogen phosphate to the potassium nitrate is (6-15) to (10-20).
Preferably, in the fully nutrient amino acid water-soluble fertilizer, the secondary element fertilizer comprises one or two of calcium ammonium nitrate and hydrated sodium silicate (generally also called instant silicon).
Preferably, in the fully nutritional amino acid water-soluble fertilizer, the secondary element fertilizer is preferably composed of calcium ammonium nitrate and sodium silicate hydrate.
Preferably, in the fully nutritional amino acid water-soluble fertilizer, the trace element fertilizer comprises one or more of boric acid, zinc nitrate and ferrous sulfate.
Preferably, in the full-nutrient amino acid water-soluble fertilizer, the trace element fertilizer consists of boric acid, zinc nitrate and ferrous sulfate.
Preferably, in the full-nutrient microbial amino acid water-soluble fertilizer, the full-nutrient microbial amino acid water-soluble fertilizer comprises 4-12 parts of silicate bacteria fermentation liquor, 4-12 parts of pediococcus pentosaceus fermentation liquor, 24-35 parts of amino acid powder, 6-15 parts of urea, 6-15 parts of monopotassium phosphate, 10-20 parts of potassium nitrate, 8-20 parts of secondary elements, 2-8 parts of trace elements and 50-70 parts of water, wherein the effective bacteria content of the silicate bacteria fermentation liquor is more than 100 hundred million/g; the content of effective bacteria in the pediococcus pentosaceus fermentation liquor is more than 50 hundred million/g, and in the amino acid powder, glycine accounts for 25 percent of the weight of the amino acid powder, and arginine accounts for 3 percent of the weight of the amino acid powder.
Preferably, in the fully nutritional amino acid water-soluble fertilizer, the secondary element fertilizer comprises one or two of calcium ammonium nitrate and sodium silicate hydrate.
Preferably, in the fully nutritional amino acid water-soluble fertilizer, the trace element fertilizer comprises one or more of boric acid, zinc nitrate and ferrous sulfate.
The invention also provides a preparation method of the full-nutrient amino acid water-soluble fertilizer, which comprises the following steps:
step 1: preparation of silicate bacteria fermentation broth
Step 2: preparation of Pediococcus pentosaceus fermentation broth
And step 3: preparation of compound microorganism amino acid-containing solution
Dissolving 25-35 parts of amino acid powder in 50-70 parts of water, and adding 4-12 parts of silicate bacteria fermentation liquor and 4-12 parts of pediococcus pentosaceus fermentation liquor to obtain the compound microorganism amino acid-containing solution.
And 4, step 4: addition of macroelements
6-15 parts of urea, 6-15 parts of monopotassium phosphate and 10-20 parts of potassium nitrate are sequentially added into the compound microorganism amino acid-containing solution, and the mixture is stirred until the urea, the monopotassium phosphate and the potassium nitrate are completely dissolved, so that the compound microorganism amino acid-containing solution containing the major elements is obtained.
And 5: chelating with medium elements
Adding 6-14 parts of calcium ammonium nitrate and 2-6 parts of instant silicon into the compound microorganism amino acid-containing solution containing the major elements, adjusting the pH value to 5-6, heating to about 40 ℃, and stirring until the mixture is completely dissolved to obtain the compound microorganism amino acid-containing solution containing the major elements.
Step 6: trace element addition chelation
Adding 1-4 parts of boric acid, 0.5-2 parts of zinc nitrate and 0.5-2 parts of ferrous sulfate into the compound microorganism amino acid-containing solution containing a large amount of medium elements, adjusting the pH value to 5.5-6.5, heating to about 40 ℃, and stirring until the mixture is completely dissolved to obtain the full-nutrient microorganism amino acid water-soluble fertilizer.
The invention also provides application of the total nutrient microbial amino acid water-soluble fertilizer, which is used for improving crop yield, crop disease resistance and content of soluble inorganic components and soluble organic components in soil.
The invention has the beneficial effects that:
the total nutrient microbial amino acid water-soluble fertilizer disclosed by the invention improves the levels of inorganic substance decomposition and organic substance decomposition in soil by virtue of the mixed fermentation broth consisting of the silicate bacteria fermentation broth and the pediococcus pentosaceus fermentation broth, and simultaneously solves the initial antagonistic function of the silicate bacteria and the pediococcus pentosaceus in the fermentation process, namely the activity ratio of two bacteria is lower due to the fact that the nutrient components of the two bacteria are in competition or the nutrition environment is not suitable in the fermentation process. The invention solves the problem by adding specific amino acid powder, realizes that the activity of the two bacteria at the initial stage is not influenced mutually, and realizes the original functions of the two bacteria.
The total nutrient microbial amino acid water-soluble fertilizer realizes promotion of crop stage production, increase of yield and reduction of diseases.
The total nutrient microbial amino acid water-soluble fertilizer realizes the degradation of inorganic matters and organic matters in soil, improves the content of soluble inorganic matters and soluble organic matters in the soil, and has excellent effect on improving the soil environment for crop growth.
Detailed Description
In the following examples, in order to verify the effect of silicate bacteria and pediococcus pentosaceus on the combination with different amino acid powders, especially the influence of the glycine and arginine contents on the performance of two fermentation liquids, amino acid powders with different glycine and arginine contents were prepared, and four kinds of amino acid powders A, B, C and D were prepared, wherein amino acid powder E was a raw product of a purchased fish meal amino acid powder.
The content of ammonium glycinate and arginine is adjusted to the amino acid coarse powder, and glycine is diluted under the condition of high arginine content and added under the condition of low glycine content in the amino acid preparation process. In general, the glycine content is insufficient, and if the arginine content needs to be adjusted to be more than 3%, the purchased pure arginine is generally required to be added.
The specific amino acid powder code and glycine and arginine content are as follows:
amino acid powder A: glycine 25% and arginine 3%
Amino acid powder B: 23 percent of ammonium glycoside and 8 percent of arginine
Amino acid powder C: glycine 15% and arginine 10%
Amino acid powder D: glycine 30% and arginine 15%
Amino acid powder E: glycine 4.52%, arginine 2.18%
In the amino acid powder, the amino acid powder E is purchased fish meal amino acid powder, wherein the content of glycine and arginine is an actual detection value. Amino acid powders A-D are to verify the effect of glycine and arginine on the effect of water soluble fertilizers.
The silicate bacteria and the pediococcus pentosaceus are both purchased from the market and are conventional silicate bacteria and pediococcus pentosaceus.
Example 1: preparation of total nutrient microbial amino acid water-soluble fertilizer I
Step 1: preparation of silicate bacteria fermentation broth
Selecting a ring of cyclosilicate bacteria by using an inoculating ring, inoculating the cyclosilicate bacteria into a seed culture medium slant test tube, and activating for 18 hours at the temperature of 37 ℃; inoculating two-ring activated silicate bacteria strain with inoculating ring into 500ml shake flask containing 150ml shake flask culture medium, culturing in constant temperature shake flask at 37 deg.C and rotation speed of 120r/min for 24 hr to obtain seed solution; inoculating the shake flask culture into a fermentation tank culture medium according to an inoculation amount of 5% by weight of the fermentation tank culture medium, and fermenting at the temperature of 35-37 ℃, the ventilation volume of 1:1, the tank pressure of 0.05MPa and the rotation speed of 120 r/min; sampling and detecting at 12h, sampling once every two hours, performing microscopic examination, and ending fermentation when the spore formation rate reaches over 90% and the effective bacteria content in the bacteria liquid is over 100 hundred million/g to obtain the silicate bacteria fermentation liquid.
The seed culture medium is prepared according to the following preparation method:
dissolving 5 parts by weight of sucrose, 2 parts by weight of potassium dihydrogen phosphate, 0.5 part by weight of magnesium sulfate heptahydrate, 0.5 part by weight of light calcium carbonate and 15 parts by weight of agar in 1000 parts by weight of distilled water, uniformly mixing, and then sterilizing at 121 ℃ for 30min to obtain the seed culture medium;
the shake flask culture medium was prepared according to the following preparation method:
dissolving 10 parts by weight of sucrose, 2 parts by weight of potassium dihydrogen phosphate, 0.5 part by weight of magnesium sulfate heptahydrate, 0.2 part by weight of sodium chloride and 1 part by weight of light calcium carbonate in 1000 parts by weight of distilled water, uniformly mixing, and then sterilizing at the temperature of 121 ℃ for 30min to obtain the shake flask culture medium;
the fermenter medium was prepared according to the following preparation method:
3 parts by weight of sucrose, 10 parts by weight of glucose, 12 parts by weight of starch, 2 parts by weight of magnesium sulfate heptahydrate, 2 parts by weight of dipotassium hydrogen phosphate, 2 parts by weight of ammonium sulfate, 2 parts by weight of precipitated calcium carbonate and 0.1 part by weight of ferric chloride were dissolved in 1000 parts by weight of distilled water, mixed uniformly, and then sterilized at 121 ℃ for 30 minutes to obtain the culture medium for the fermentation tank.
Step 2: preparation of Pediococcus pentosaceus fermentation broth
Selecting a pediococcus pentosaceus strain by using an inoculating loop, inoculating the pediococcus pentosaceus strain into a seed culture medium slant test tube, and activating at the temperature of 37 ℃ for 18 hours; inoculating two-ring activated pediococcus pentosaceus strain to a 500ml shake flask containing 150ml shake flask culture medium, and culturing in a constant temperature shaking table at 37 deg.C and rotation speed of 120r/min for 24 hr to obtain seed solution; the shake flask culture was inoculated into a fermenter medium at an inoculum size of 3% by weight of the fermenter medium, and the fermentation was carried out at 35-37 ℃ under a tank pressure of 0.04mpa and a rotation speed of 200 rpm/min. And finishing fermentation when the effective bacteria content in the bacteria liquid is more than 50 hundred million/g to obtain the pediococcus pentosaceus fermentation liquid.
The seed culture medium is prepared according to the following preparation method:
dissolving 2 parts by weight of glucose, 0.5 part by weight of yeast powder, 1 part by weight of beef extract, 1 part by weight of peptone, 0.05 part by weight of magnesium sulfate and 0.005 part by weight of manganese sulfate in 1000 parts by weight of distilled water, uniformly mixing, and then sterilizing at the temperature of 121 ℃ for 30min to obtain the seed culture medium.
The shake flask culture medium was prepared according to the following preparation method:
dissolving 2 parts by weight of glucose, 0.5 part by weight of yeast powder, 1 part by weight of beef extract, 1 part by weight of peptone, 0.5 part by weight of sodium acetate, 0.1 part by weight of monopotassium phosphate, 0.2 part by weight of diammonium hydrogen citrate, 0.052 part by weight of magnesium sulfate, 0.005 part by weight of manganese sulfate, 801ml of tween and 1 part by weight of light calcium carbonate in 1000 parts by weight of distilled water, uniformly mixing, and then sterilizing at the temperature of 121 ℃ for 30min to obtain the shake flask culture medium.
The fermenter medium was prepared according to the following preparation method:
3.5 parts by weight of glucose, 1.0 part by weight of yeast powder, 1.0 part by weight of beef extract, 0.6 part by weight of sodium acetate, 0.2 part by weight of monopotassium phosphate, 0.2 part by weight of diammonium hydrogen citrate, 0.05 part by weight of magnesium sulfate, 0.006 part by weight of manganese sulfate, 1 part by weight of precipitated calcium carbonate and 801.5ml of tween are dissolved in 1000 parts by weight of distilled water, uniformly mixed and then sterilized at 121 ℃ for 30min to obtain the fermentation tank culture medium.
And step 3: preparation of compound microorganism amino acid-containing solution
And (3) dissolving 30 parts of amino acid powder A in 60 parts of water, and adding 8 parts of silicate bacteria fermentation liquor and 8 parts of pediococcus pentosaceus fermentation liquor to obtain the compound microorganism amino acid-containing solution.
And 4, step 4: addition of macroelements
And sequentially adding 10 parts of urea, 10 parts of monopotassium phosphate and 15 parts of potassium nitrate into the compound microorganism amino acid-containing solution, and stirring until the urea, the monopotassium phosphate and the potassium nitrate are completely dissolved to obtain the compound microorganism amino acid-containing solution containing the major elements.
And 5: chelating with medium elements
And adding 10 parts of calcium ammonium nitrate and 4 parts of instant silicon into the compound microorganism amino acid-containing solution containing the major elements, adjusting the pH value to 5-6, heating to about 40 ℃, and stirring until the mixture is completely dissolved to obtain the compound microorganism amino acid-containing solution containing a large amount of medium elements.
Step 6: trace element addition chelation
Adding 3 parts of boric acid, 1 part of zinc nitrate and 1 part of ferrous sulfate into the compound microorganism amino acid-containing solution containing a large amount of medium elements, adjusting the pH value to 5.5-6.5, heating to about 40 ℃, and stirring until complete dissolution to obtain the full-nutrition microorganism amino acid water-soluble fertilizer I.
Example 2: preparation of total nutrient microbial amino acid water-soluble fertilizer II
According to the method of example 1, the amino acid powder A is replaced by the amino acid powder B, and the other preparation conditions are unchanged, so that the total-nutrient microbial amino acid water-soluble fertilizer II is obtained.
Example 3: preparation of total nutrient microbial amino acid water-soluble fertilizer III
According to the method of example 1, the amino acid powder A is replaced by the amino acid powder C, and the other preparation conditions are unchanged, so that the total nutrient microbial type amino acid water-soluble fertilizer III is obtained.
Example 4: preparation of total nutrient microbial amino acid water-soluble fertilizer IV
According to the method of example 1, the amino acid powder A is replaced by the amino acid powder D, and the rest preparation conditions are unchanged, so that the total nutrient microbial amino acid water-soluble fertilizer IV is obtained.
Example 5: preparation of full-nutrient microbial amino acid water-soluble fertilizer V
According to the method of example 1, the amino acid powder A is replaced by the amino acid powder E, and the rest preparation conditions are unchanged, so that the total-nutrient microbial amino acid water-soluble fertilizer V is obtained.
Example 6: preparation of total nutrient microbial amino acid water-soluble fertilizer VI
According to the method of example 1, the amino acid powder A is replaced by 30 parts of distilled water, and the rest preparation conditions are unchanged, so that the total nutrient microbial type amino acid water-soluble fertilizer VI is obtained.
The prepared total-nutrient microbial amino acid water-soluble fertilizer I-VI is analyzed and determined according to a standard analysis method of NY884-2012 bioorganic fertilizer, and the result shows that the total effective viable count is more than or equal to 5.4 hundred million/g; the organic matter content is more than or equal to 15.4 percent by weight; performing analysis and determination according to a standard analysis method of NY1107-2010 macroelement water-soluble fertilizer, wherein nitrogen, phosphorus and potassium are more than or equal to 20.8%; according to the analysis and determination of a standard analysis method of NY1429-2010 amino acid-containing water-soluble fertilizer, the content of calcium and silicon is more than or equal to 3.5 percent; the content of B, Zn and Fe is more than or equal to 0.5 percent.
Test example 1: wheat application test of total-nutrient microbial amino acid water-soluble fertilizer
The implementation of this test example is as follows:
the test varieties are as follows: wheat, Zhoumai 22.
Test time: 10/2/2015 to 2016/6/30/2015.
Test site: the city of Job's work in Henan province, Xiu county, Zhou Zhuang, county, Meng village.
The test method comprises the following steps: the experiment is provided with 8 treatments, each treatment is repeated for 3 times, 24 cells are arranged in total, and the area of each cell is 30m2. The experimental topdressing and other management measures are the same.
Treatment 1: conventional fertilization and spraying of 400 times of a total nutrient microbial amino acid water-soluble fertilizer I, wherein the spraying is performed once in a node pulling period, a booting period and a grouting period;
and (3) treatment 2: conventional fertilization and spraying of 400 times of a total nutrient microbial amino acid water-soluble fertilizer II, wherein the spraying is performed once in a node pulling period, a booting period and a grouting period;
and (3) treatment: conventional fertilization and spraying of 400-fold liquid of the total nutrient microbial amino acid water-soluble fertilizer III, wherein the liquid is sprayed once in the jointing stage, the booting stage and the grouting stage;
and (4) treatment: conventional fertilization and spraying of 400 times of a total nutrient microbial amino acid water-soluble fertilizer IV solution, wherein the solution is sprayed once in a node pulling period, a booting period and a grouting period;
and (4) treatment 5: conventional fertilization and spraying of 400 times of a total nutrient microbial amino acid water-soluble fertilizer V solution, wherein the solution is sprayed once in a node pulling period, a booting period and a grouting period;
and (6) treatment: conventional fertilization and spraying of 400 times of a total nutrient microbial amino acid water-soluble fertilizer VI, wherein the spraying is performed once in a node pulling period, a booting period and a grouting period;
and (7) treatment: conventional fertilization and spraying of a commercially available amino acid liquid fertilizer in the same period as the treatment 1;
and (4) treatment 8: conventional fertilization and spraying clean water with the same amount as that of the treatment 1 in the same period;
the test was performed on a regular fertilization basis. The conventional fertilization comprises the following steps: 50kg of compound fertilizer is applied to the bottom of each mu of the fertilizer, and 15kg of urea is applied to each mu of the fertilizer in the jointing stage. And strictly according to the requirements of the test scheme, the full-nutrient microbial amino acid water-soluble fertilizer, the commercially available amino acid liquid fertilizer and clear water are respectively sprayed in 2017 for 3 and 30 days, 4 and 18 days and 5 and 8 days. During harvesting, each cell is singly harvested and called to count the yield, and field investigation and seed test are simultaneously carried out. The other management measures of the test are the same as the general field production management except that the fertilizer liquid or clear water is applied by water according to the scheme.
1. Influence on growth period of wheat
The wheat growth period is investigated and shown in table 1, and the spraying of the total nutrient microbial amino acid water-soluble fertilizer has a certain promotion effect on the wheat growth period, which is mainly shown in that the indexes of the elongation stage, the heading stage and the maturation stage are improved to different degrees.
TABLE 1 questionnaire of wheat growth period
| Growth period | Process 1 | Treatment 2 | Treatment 3 | Treatment 4 | Treatment 5 | Treatment 6 | Treatment 7 | Treatment 8 |
| Sowing time | 16/10 | 16/10 | 16/10 | 16/10 | 16/10 | 16/10 | 16/10 | 16/10 |
| Stage of emergence | 24/10 | 24/10 | 24/10 | 24/10 | 24/10 | 24/10 | 24/10 | 24/10 |
| Tillering stage | 22/11 | 22/11 | 22/11 | 22/11 | 22/11 | 22/11 | 22/11 | 22/11 |
| Jointing stage | 28/3 | 28/3 | 28/3 | 28/3 | 28/3 | 29/3 | 29/3 | 30/3 |
| Heading period | 19/4 | 19/4 | 19/4 | 19/4 | 20/4 | 21/4 | 23/4 | 25/4 |
| Maturity stage | 4/6 | 5/6 | 6/6 | 6/6 | 7/6 | 8/6 | 12/6 | 16/6 |
The time for advancing the maturation period was the longest in the early and late stages of each growth period using the fully nutrient microbial amino acid water-soluble fertilizer of example 1, and the heading period was advanced by at least 5 days using the fully nutrient microbial amino acid water-soluble fertilizers of examples 1 to 5 of the present invention.
2. Influence on major diseases of wheat
TABLE 2 investigation of major wheat diseases
Wheat sharp eyespot and powdery mildew are main diseases of wheat in China and become important factors influencing wheat production. After the general outbreak of the control plot, the treatments are investigated, the investigation of the sheath blight and powdery mildew of wheat is shown in table 2, and the results show that when the full-nutrient microbial amino acid water-soluble fertilizer of the embodiment 1 of the invention is sprayed, the control effects on the sheath blight and the powdery mildew of wheat are 42.69% and 57.47% respectively, which shows that the compound microbial liquid contained in the fertilizer to be tested plays a good role in inhibiting the expansion and spread of the main diseases of wheat.
3. Influence on wheat production factor
The spraying of the full-nutrient microbial amino acid water-soluble fertilizer improves the yield factor of wheat. As can be seen from table 3: treatment 1 had an increase in grain size per ear and an increase in thousand grain weight resulted in an increase in yield per mu as compared with treatments 2-8. The fact that the total nutrient microbial amino acid water-soluble fertilizer of the embodiment 1 of the invention is sprayed can increase the ear grain number and thousand grain weight of wheat, increase the yield per mu and realize that the yield increase ratio of the estimated yield per mu reaches 19 percent, which is almost peculiar to the production of wheat.
TABLE 3 statistical table for field investigation and seed examination
4. Improvement of wheat soil
By preliminary calculation from measurements of the soil for the unit soluble organic matter content and the unit soluble inorganic matter, treatment 1 of the total nutrient microbial amino acid water-soluble fertilizer of example 1, which are higher than the treatment 2-8, was applied, and the total dry weight of the soluble organic matter and the soluble inorganic matter in the soil was measured during the maturation period, and it was found that treatment 1 was increased by 4.32% from the total dry weight of the soil soluble organic matter and the soluble inorganic matter of treatment 2 and by 12.98% from the total dry weight of the soil soluble organic matter and the soluble inorganic matter of treatment 8. It can be seen that the invention has an unexpected soil improvement effect through the content adjustment of glycine and arginine in the amino acid powder.
Claims (8)
1. The full-nutrition amino acid water-soluble fertilizer comprises mixed fermentation liquor and amino acid powder, wherein the mixed fermentation liquor consists of silicate bacteria fermentation liquor and pediococcus pentosaceus fermentation liquor, the effective bacteria content of the silicate bacteria fermentation liquor is more than 100 hundred million/g, the effective bacteria content of the pediococcus pentosaceus fermentation liquor is more than 50 hundred million/g, the weight ratio of the silicate bacteria fermentation liquor to the pediococcus pentosaceus fermentation liquor is (1-3) to (1-3), the weight of the amino acid powder is 1-5 times of that of the mixed fermentation liquor, in the amino acid powder, the weight percentage of glycine is 23-50% of the weight of the amino acid powder, and the weight percentage of arginine is 1-8% of the weight of the amino acid powder.
2. The full nutritional type amino acid water soluble fertilizer as claimed in claim 1, characterized in that the full nutritional type amino acid water soluble fertilizer further comprises a macroelement fertilizer, a secondary element fertilizer and a microelement fertilizer, wherein the weight of the macroelement fertilizer is 1-6 times of the weight of the mixed fermentation broth, the weight of the secondary element fertilizer is 0.3-3 times of the weight of the mixed fermentation broth, and the weight of the microelement fertilizer is 0.1-1 times of the weight of the mixed fermentation broth.
3. The full nutritional type amino acid water soluble fertilizer as claimed in claim 2, characterized in that the macroelement fertilizer consists of urea, monopotassium phosphate and potassium nitrate, and the weight ratio of urea, monopotassium phosphate and potassium nitrate is (6-15): (10-20).
4. The fully nutritive amino acid water soluble fertilizer according to claim 2, characterized in that the secondary element fertilizer comprises one or both of calcium ammonium nitrate and sodium silicate hydrate.
5. The fully nutritive amino acid water soluble fertilizer according to claim 2, characterized in that the trace element fertilizer comprises one or more of boric acid, zinc nitrate and ferrous sulphate.
6. The full-nutrition microbial amino acid water-soluble fertilizer is characterized by comprising 4-12 parts of silicate bacteria fermentation liquor, 4-12 parts of pediococcus pentosaceus fermentation liquor, 24-35 parts of amino acid powder, 6-15 parts of urea, 6-15 parts of monopotassium phosphate, 10-20 parts of potassium nitrate, 8-20 parts of secondary elements, 2-8 parts of trace elements and 50-70 parts of water, wherein the effective bacteria content of the silicate bacteria fermentation liquor is more than 100 hundred million/g; the content of effective bacteria in the pediococcus pentosaceus fermentation liquor is more than 50 hundred million/g, and in the amino acid powder, glycine accounts for 25 percent of the weight of the amino acid powder, and arginine accounts for 3 percent of the weight of the amino acid powder.
7. The fully nutritive amino acid water soluble fertilizer according to claim 6, characterized in that the secondary element fertilizer comprises one or both of calcium ammonium nitrate and sodium silicate hydrate and the trace element fertilizer comprises one or more of boric acid, zinc nitrate and ferrous sulfate.
8. Use of the complete nutrient microbial amino acid water-soluble fertilizer as claimed in claim 6 or 7, for increasing wheat yield, wheat disease resistance and increasing the content of soluble inorganic components and soluble organic components in soil.
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