CN108083597A - A kind of processing anaerobically digested sludge complex microorganism bacterium solution and bioleaching new method - Google Patents
A kind of processing anaerobically digested sludge complex microorganism bacterium solution and bioleaching new method Download PDFInfo
- Publication number
- CN108083597A CN108083597A CN201711449769.1A CN201711449769A CN108083597A CN 108083597 A CN108083597 A CN 108083597A CN 201711449769 A CN201711449769 A CN 201711449769A CN 108083597 A CN108083597 A CN 108083597A
- Authority
- CN
- China
- Prior art keywords
- sludge
- bioleaching
- anaerobically digested
- digested sludge
- bacterium solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/12—Treatment of sludge; Devices therefor by de-watering, drying or thickening
- C02F11/121—Treatment of sludge; Devices therefor by de-watering, drying or thickening by mechanical de-watering
- C02F11/122—Treatment of sludge; Devices therefor by de-watering, drying or thickening by mechanical de-watering using filter presses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Mycology (AREA)
- Mechanical Engineering (AREA)
- Molecular Biology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention discloses a kind of bioleaching methods of anaerobically digested sludge, belong to technical field of sludge treatment.The present invention is re-dubbed bioleaching work flora by introducing new microbial strains, and it is constantly tamed, the inoculum obtained will be tamed to carry out mixing progress bioleaching processing with anaerobically digested sludge, the EPS for being wrapped in sludge cell surface is quickly removed using new microbial strains degradation of small molecular organic acid and secretion surface reactive material, the bioleaching reaction time foreshortens to 12~for 24 hours, sludge is directly dehydrated into plate and frame filter press after bioleaching, obtains the mud cake that moisture content is less than 60%.This method is simple for process, and full utilization microbial action need not add any chemical agent, has the advantages that dehydration efficiency is high, operating cost is low, technological operation difficulty is small.
Description
Technical field
It is compound micro- more specifically to a kind of processing anaerobically digested sludge the invention belongs to technical field of sludge treatment
Biological bacterium solution and bioleaching new method.
Background technology
In recent years, with the quickening of Urbanization in China and the raising of environmental quality standards, pending dirt is caused
Water sharply increases, therefore various sewage treatment process such as oxidation ditch, biofilter and oxidation pond is used widely at this stage,
Ultimately result in a large amount of generations of another pollutant (sludge) in sewage disposal.The ingredient of sludge is sufficiently complex, separate sources
Sludge components difference is huge, and sludge mainly includes the zoogloea of multiple-microorganism formation and its organic and inorganic substances group of absorption
Into set, effective processing and disposal are only carried out to sludge in time, just can guarantee the operation of sewage treatment plant and processing effect
Fruit, specific resistance to filtration become become increasingly conspicuous and urgent need to resolve potential risk and environmental problem.Sludge dewatering can effectively drop
The volume of low pending mud, be a kind of method the most economic in sludge reduction and sludge ultimate disposal as burned, heap
The essential link such as fertilizer.
Currently used deep dehydration method for sludge mainly uses and organic or inorganic flocculating agent, nothing is added into sludge system
Machine flocculant mainly has molysite, aluminium salt etc., and organic flocculant mainly has cationic high-molecular flocculant (PAM-DMC) and poly- third
Acrylamide (PAM).Polyacrylamide is the widely used flocculant of present sewage treatment plant, mostly common with lime and ferric trichloride
It uses, moisture content is then dewatered to below 60% using chamber-type press filter.This method finds many deficiencies in practical applications, 1.
Operating cost is high, and inorganic dosing is larger, it is necessary to reach the 60% of sludge dry matter；2. treated, sludge calorific value significantly drops
It is low, it is unfavorable for the utilization of sludge following resourceization；After 3. lime stone adds in simultaneously, COD and ammonia-nitrogen content can be made to increase, improve sewage
Load is handled, sludge pH is promoted to 12-13.
Bioleaching technology is the deeply dehydrating sludge new technology newly risen in recent years, and sludge handling process full utilization is given birth to
Object drip soaks exclusive microorganism and carries out dewatering modification to sludge under aeration condition, through the direct press filtration of plate and frame filter press to aqueous
Mud cake of the amount less than 60%, has that operating cost is low, save sludge organic matter from damage and calorific value, will not increase dewatered sludge dry matter
Amount need not add any flocculant or flocculation aid, the advantage for being completely removed sludge stench and pathogen using microorganism completely,
It is a kind of environmentally friendly Sludge Disposal Techniques.
In view of clear superiority of the bioleaching technology on deeply dehydrating sludge, is disposed in sludge from wastewater treatment plant
In be used widely, Treatment of Sludge engineering is formed on Wuxi, Harbin, Tongxiang, Ningbo and other places, handles sludge day up to 30,000,000
Ton.Through retrieval, Chinese Patent Application No. 201410119833.X, data of publication of application is the patent application on July 23rd, 2014
File discloses the new method that a kind of sludge bioleaching method quickly nurses one's health deep dehydration, and this method carries out biology to sludge first
Drip leaching conditioning, i.e., directly drip sewage treatment plant's concentrated liquid sludge pumping to the biology containing dedicated complex microbial community
1.5-2d is handled in leaching reactor, irreducible water in sludge is made by the effect of microorganism substitution effect, biological oxidation and biological acidification
Become to be easier to the Free water removed, while Heavy Metals in Sludge is made to be extracted into water phase and kills pathogen in sludge.Then will
Sludge after bioleaching is collected in homogeneous pond, then is delivered directly in the membrane chamber-type press filter of usual pressure and taken off by pump
Water, dewatered sludge cake moisture content are less than 60%.The complex microbial community used in the patent is Thiobacilus thiooxidans TS6, oxygen
Change ferrous Thiobacillus LX5, Pichia pastoris Z3 and rhodotorula R30, applicable environment is indigested ordinary municipal sludge, is such as concentrated
Sludge, such sludge can be reduced quickly through bioleaching processing sludge pH, are helped to reduce the negative electrical charge on mud granule surface, be reached
To the effect of repulsion between decrease mud granule, the final enhancing for being beneficial to dehydration property achievees the effect that deep dehydration.Anaerobism
Digestion is the technical process for the sludge stabilizing that sewage treatment plant generally uses, and the purpose is to eliminate pathogen, reduce volatility
Solid and removal stench.Anaerobic digestion is divided into three phases, i.e. hydrolysed ferment stage, hydrogenesis and acetogenesis stage and methane phase rank
Section, hydrolyzing sludge can increase methane production before digestion, it may be difficult to which the organic molecule and molecular complex of decomposition are degraded to small
Molecular organic acids, such as formic acid, acetic acid, propionic acid, butyric acid, the sludge after anaerobic digestion contain suspended matter, ammonia nitrogen, phosphorus and substantial amounts of
Extracellular polymers (EPS), sludge pH is in alkalescence etc..In practice, it has been found that directly handled using traditional biological drip leaching method
Anaerobically digested sludge is unable to reach desired effect, and dewatering cannot improve, and resistivity rises instead with processing time, sludge
System pH does not drop to be risen instead.
Since traditional biological drip soaking technology can not improve anaerobically digested sludge dewatering, the effect of deep dehydration is not achieved
Fruit, therefore a kind of bioleaching new method suitable for anaerobically digested sludge is urgently found, to improve bioleaching dehydration efficiency,
It realizes and the target that deep dehydration realizes minimizing is carried out to anaerobically digested sludge.
The content of the invention
1. it to solve the problems, such as
Sludge specific resistance, the pH occurred when directly handling anaerobically digested sludge for existing traditional biological drip leaching method does not drop instead
The phenomenon that rising causes sludge dewatering effect to deteriorate, the present invention provide a kind of bioleaching new method of anaerobically digested sludge, adopt
The composite bacteria liquid pair formed with Thiobacilus ferrooxidans LX5, Thiobacilus thiooxidans TS6, Bordetella ZW2, Pichia pastoris D13
Anaerobically digested sludge carries out bioleaching.
2. technical solution
To solve the above-mentioned problems, the technical solution adopted in the present invention is as follows：
A kind of bioleaching new method of anaerobically digested sludge, comprises the following steps：
(1) complex microorganism bacterium solution culture
By the Thiobacilus ferrooxidans LX5 isolated from sludge (A.ferrooxidans LX5, preserving number CGMCC
NO.0727), Thiobacilus thiooxidans TS6 (A.thiooxidans TS6, preserving number CGMCC NO.0759), Bordetella ZW2
(Bordetella sp. preserving number CGMCC NO.3033), Pichia pastoris D13 (P.spartinae D13, preserving number CGMCC
NO.3035) it is seeded to respectively in respective culture medium, is subsequently placed in 23~28 DEG C, vibrates in 180~200rpm reciprocal shakers and expand
Numerous culture, until somatic cells quantity reaches 108A/mL；Then by Thiobacilus ferrooxidans LX5, Thiobacilus thiooxidans TS6,
Bordetella ZW2, Pichia pastoris D13 culture solutions are by volume (1~3)：(2~5)：(1~3)：(1~3) mixing is answered
Close microbial inoculum；
(2) complex microorganism bacterium solution is tamed
Complex microorganism bacterium solution is seeded in anaerobically digested sludge system, microbial nutrition microbial inoculum is added, 23~28
DEG C, carry out culture domestication under aeration condition, until sludge system pH, which is down to 3.0 stopping cultures, obtains first batch of acidification sludge；Then
First batch of acidification sludge is inoculated to fresh anaerobically digested sludge, adds microbial nutrition microbial inoculum, in 23~28 DEG C, aeration item
Culture domestication is carried out under part, until sludge system pH is down to 3.0 stopping cultures；Repeat the above process to complex microorganism bacterium solution into
Row domestication；
(3) bioleaching is nursed one's health
By the complex microorganism bacterium solution of domestication and anaerobically digested sludge by volume 1：(5~10) are mixed to biology and are dripped
Soak in reactor, add in microbial nutrition microbial inoculum, 23~28 DEG C, aeration, bioleaching 12 under stirring condition~for 24 hours, obtain
Bioleaching sludge；
(4) filter-press dehydration
The direct press filtration under plate and frame filter press of sludge after bioleaching is nursed one's health carries out separation of solid and liquid, and it is low to obtain moisture content
In 60% sludge cake.
Further, Thiobacilus ferrooxidans LX5 culture medium g/L：(NH4)2SO43.5, KCl 0.119,
K2HPO40.58, Ca (NO3)2·4H2O 0.168, MgSO4·7H2O 4.42, with 10N H2SO4Adjust pH to 2.5；Sulfur oxide sulphur bar
Bacterium TS6 culture mediums g/L：(NH4)2SO40.4, KH2PO43.0, MgSO4·7H2O 0.5, CaCl2·2H2O 0.25, sulphur powder 10；
Bordetella ZW2 culture mediums g/L：(NH4)2SO40.3, CaCl2·2H2O 0.3, KCl 0.5, ZnSO40.3, MnSO4
0.23；Pichia pastoris D13 culture mediums g/L：Mealy potato 200, glucose 20, with 10N H2SO4Adjust pH to 2.5.
Further, the microbial nutrition microbial inoculum is to use high nutrient as raw material, after microbial fermentation
After filter-press dehydration, drying crushes obtained solid matter, main nutrient elements include active thalline and containing N, P, K and Fe, Zn,
The nutrients such as Mn, S.
Further, the microbial nutrition microbial inoculum adds volume as the 2~5% of anaerobically digested sludge.
Further, complex microorganism bacterium solution is connect by the 1~10% of anaerobically digested sludge volume in step (2)
Kind；3-5 times is repeated the above process in step (2) to tame complex microorganism bacterium solution.
Further, the aeration condition refers to aeration control in 3~6m3/(h·m2)。
Further, the anaerobically digested sludge moisture control is more than 98%.
A kind of complex microorganism bacterium solution for handling anaerobically digested sludge, by Thiobacilus ferrooxidans LX5, Thiobacillus thioxidans
TS6, Bordetella ZW2, the compound compositions of Pichia pastoris D13.
Further, Thiobacilus ferrooxidans LX5, Thiobacilus thiooxidans TS6, Bordetella ZW2, Pichia pastoris
The thalline quantity ratio of D13 is (1~3)：(2~5)：(1~3)：(1~3).
Applications of the Bordetella ZW2 (Bordetella sp.) in anaerobically digested sludge process field.
The bioleaching reactor is pulling flow type bioleaching reactor, and preferably Application No. 200810242584.8 is special
Pulling flow type bioleaching processing equipment disclosed in profit application.
3. advantageous effect
Compared with the prior art, beneficial effects of the present invention are：
(1) present invention is re-dubbed bioleaching work flora by introducing new microbial strains, and is constantly tamed and dociled
Change, quickly removed using new microbial strains Bordetella ZW2 degradation of small molecular organic acid and secretion surface reactive material
The EPS of sludge cell surface is wrapped in, eliminate the murder by poisoning inhibitory action to bioleaching acidophilic thiobacillus and is disappeared in anaerobism
Change and quickly bred in sludge system, play biological acidification effect, ensure bioleaching dehydration efficiency.
(2) conditioning of present invention processing anaerobically digested sludge bioleaching only need 12~for 24 hours, compared with compared with traditional biological drip leaching
Conditioning shortens 12~for 24 hours, directly reduce investment operating cost.
(3) present invention uses energy substance of the microbial nutrition microbial inoculum as composite flora, and utilization rate is high, and usage amount is few,
50%~80% is reduced compared with traditional microbiological nutritional agents, effectively reduces bioleaching operating cost.
(4) complex microbial community after domestication is added directly into sludge by the present invention, without individually cultivating, is simplified
Technical process and reduction actual motion operation difficulty.
(5) complex microorganism after being tamed in the present invention only need to be added once, longtime running, save operating cost.
(6) present invention process is simple, completely using microbial process, without adding any chemical agent, does not influence sludge
The key indexes such as calorific value, organic matter, it can be achieved that following resourceization utilizes diversity, generate certain economic value.
(7) acidophilic thiobacillus can not survive under alkaline environment, inanimate object acidification, and it is dirty that bioleaching does not have improvement
Mud dehydrating effect；The study found that Bordetella ZW2 (the Bordetella sp. preserving numbers CGMCC isolated from sludge
NO.3033) degradable small molecule organic material and secrete surface reactive material and quickly remove EPS, including may be by cell cracking
Albumen, polysaccharide, the nucleic acid of generation play an important role to improving sludge dewatering effect.
Description of the drawings
Fig. 1 is the bioleaching new method process flow chart of anaerobically digested sludge in the present invention.
Biomaterial preservation proves
Thiobacilus ferrooxidans LX5, classify entitled thiobacillus ferrooxidans, is preserved in China Microbiological
Culture presevation administration committee common micro-organisms center, preservation address are Chinese Zhong Guan-cun, and preservation date is March 13 in 2002
Day, preserving number CGMCCNo.0727.
Thiobacilus thiooxidans TS6, classify entitled Thiobacillus thiooxidans, is preserved in Chinese microorganism strain
Preservation administration committee common micro-organisms center, preservation address are Chinese Zhong Guan-cun, and preservation date is on July 4th, 2002, preservation
Number be CGMCCNo.0759.
Pichia pastoris D13, classify entitled Pichia spartinae, is preserved in Chinese microorganism strain preservation conservator
Can common micro-organisms center, preservation address is Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, preservation date
For on April 23rd, 2009, preserving number was CGMCC No.3035.
Bordetella ZW2, classify entitled Bordetella sp., is preserved in Chinese microorganism strain preservation conservator
Can common micro-organisms center, preservation address is Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, preservation date
For on April 23rd, 2009, preserving number CGMCC NO.3033.
Specific embodiment
The present invention is further described below with reference to specific embodiment.
Embodiment 1
The anaerobically digested sludge bioleaching processing experiment of Nanjing sewage treatment plant
Mentioned technique according to the present invention, according to flow (see attached drawing 1) to sewage treatment plant's anaerobically digested sludge into
Row processing.Qu Yue20LGai factories anaerobically digested sludge, sludge fundamental property is as follows after measured：PH value is 7.10, solid content
4.06%, organic matter 48.9%, sludge specific resistance 6.8 × 1013M/kg, total nitrogen 35.1%, total phosphorus 27%.
(1) anaerobically digested sludge culture complex microbial community is utilized
By Thiobacilus ferrooxidans LX5 (A.ferrooxidans LX5, preserving number are CGMCC NO.0727), sulfur oxide
Thiobacillus TS6 (A.thiooxidans TS6, preserving number CGMCC NO.0759), Bordetella ZW2 (Bordetella sp.
Preserving number CGMCC NO.3033), Pichia pastoris D13 (P.spartinae D13, preserving number CGMCC NO.3035) is inoculated with respectively
(the Thiobacilus ferrooxidans LX5 culture medium g/L into respective culture medium：(NH4)2SO43.5, KCl 0.119, K2HPO40.58,
Ca(NO3)2·4H2O 0.168, MgSO4·7H2O 4.42, with 10N H2SO4Adjust pH to 2.5；Thiobacilus thiooxidans TS6 culture
Base g/L：(NH4)2SO40.4, KH2PO43.0, MgSO4·7H2O 0.5, CaCl2·2H2O 0.25, sulphur powder 10；Bordetella
Bacterium ZW2 culture mediums g/L：(NH4)2SO40.3, CaCl2·2H2O 0.3, KCl 0.5, ZnSO40.3, MnSO40.23；Bi Chi
Yeast D13 culture mediums g/L：Mealy potato 200, glucose 20, with 10N H2SO4PH is adjusted to 2.5), being subsequently placed in 25 DEG C, 180rpm
Numerous culture is expanded in vibration in reciprocal shaker, until somatic cells quantity reaches 108A/mL.By Thiobacilus ferrooxidans LX5, oxygen
Change sulphur Thiobacillus TS6, Bordetella ZW2, Pichia pastoris D13 culture solutions by volume 2：4：3：3 mixing obtain composite microbial
Complex microorganism bacterium solution is seeded in anaerobically digested sludge system (bacterium solution volume 10mL, anaerobically digested sludge body by object bacterium solution
Product is 100mL), microbial nutrition microbial inoculum 2g is added, (the aeration quantity 3m under 25 DEG C, aeration condition3/(h·m2)) cultivate and tame and docile
Change, until sludge system pH, which is down to 3.0 stopping cultures, obtains first batch of acidification sludge.Then first batch of acidification sludge 100mL is taken to connect again
Kind adds microbial nutrition microbial inoculum the 2g, (aeration quantity under 25 DEG C, aeration condition to fresh 1000mL anaerobically digested sludges
3m3/(h·m2)) culture domestication is carried out, until sludge system pH is down to 3.0 stopping cultures.It repeats the above process 5 times, gained acid
Change sludge as inoculum after domestication.
The preparation method of microbial nutrition microbial inoculum is as follows：
(a) complex microbial community is obtained
Thiobacilus ferrooxidans LX5 (A.ferrooxidans LX5, preserving number are CGMCC NO.0727), Boulder is special
Salmonella ZW2 (Bordetella sp., preserving number CGMCC NO.3033) is seeded to respectively in respective culture medium, is subsequently placed in 25
DEG C, (3m under aeration condition3/(h·m2)) expand numerous culture, until somatic cells quantity reaches 107A/mL.By ferrous oxide sulphur bar
Bacterium LX5, Bordetella ZW2 culture solutions by volume 3：1 mixing obtains complex microorganism bacterium solution.Complex microorganism bacterium solution is overall
Product is 5L.
(b) nutriment composite flora ferments
Weigh sucrose 50g, peptone 10g, potassium dihydrogen phosphate 100g, ammonium chloride 40g, phosphoric acid hydrogen ammonia 10g, ammonium sulfate 50g,
Bitter salt 30g, five water sodium thiosulfate 50g, calcium nitrate 10g, ferric sulfate 50g, manganese sulfate 10g, zinc sulfate 100g, chlorine
Change potassium 40g, green vitriol 200g, ammonium sulfate 10g, sulphur 50g.
Above-mentioned nutriment is dissolved in 10L water, adds in complex microorganism the bacterium solution, (3m under 25 DEG C, aeration condition3/
(h·m2)) cultivated, until cultivating system pH stops culture when being reduced to 3.0.
(c) separation of solid and liquid
Mixture after composite flora ferments is separated through solid-liquid separating equipment, and will be Nutrious fermented using crushing plant
Below cake crushing to 100 mesh.
(d) auxiliary material mixes
Nutrious fermented object and filler material diatomite are put into blender, is sufficiently mixed, stirs evenly, obtains dietetic bacterial
Agent.
(2) bioleaching is handled
Pending anaerobically digested sludge is diluted to moisture content 98%, according to the volume ratio of inoculum and anaerobically digested sludge
1：10 are put into inoculum and anaerobically digested sludge to bioleaching reactor, (the aeration quantity 3m under 25 DEG C, aeration condition3/
(h·m2)) bioleaching 12h, the modified sludge of bioleaching is obtained, sludge system pH is measured and is down to 2.0.
(3) filter-press dehydration
The direct press filtration of plate and frame filter press, feed time 30min, high pressure will be delivered to after bioleaching is nursed one's health by sludge pump
Hydraulic pressure squeezes pressurize 45min, feed pressure 0.6MPa, diaphragm pressing pressure 1.0MPa, and press filtration water colorless is clarified at this time, is squeezed out
Mud cake is in khaki, and odorless, moisture content of the cake is 58.4% after measured.
Comparative example 1
Leaching microorganism species (not adding Bordetella ZW2) are dripped using traditional biological and handle above-mentioned anaerobically digested sludge,
Processing step is nursed one's health, sludge system pH is 8.6, and sludge pump is delivered to plate compression with embodiment 1 through bioleaching for 24 hours
The direct press filtration of machine, 1h inputiing mud volumes are 20L, and the mud cake squeezed out is shapeless, black, there is stink, and moisture content of the cake exists after measured
85%, press filtration water is muddy slime water.
Embodiment 2
The anaerobically digested sludge bioleaching processing experiment of Wuxi sewage treatment plant
Mentioned technique according to the present invention, according to flow (see attached drawing 1) to sewage treatment plant's anaerobically digested sludge into
Row processing.About 100L factory's anaerobically digested sludges are taken, sludge fundamental property is as follows after measured：PH value is 7.56, solid content
3.0%, organic matter 50.1%, sludge specific resistance 8.37 × 1013M/kg, total nitrogen 35.1%, total phosphorus 27%.
(1) anaerobically digested sludge culture complex microbial community is utilized
Thiobacilus ferrooxidans LX5, Thiobacilus thiooxidans TS6, Bordetella ZW2, Pichia pastoris D13 are inoculated with respectively
(the Thiobacilus ferrooxidans LX5 culture medium g/L into respective culture medium：(NH4)2SO43.5, KCl 0.119, K2HPO40.58,
Ca(NO3)2·4H2O 0.168, MgSO4·7H2O 4.42, with 10N H2SO4Adjust pH to 2.5；Thiobacilus thiooxidans TS6 culture
Base g/L：(NH4)2SO40.4, KH2PO43.0, MgSO4·7H2O 0.5, CaCl2·2H2O 0.25, sulphur powder 10；Bordetella
Bacterium ZW2 culture mediums g/L：(NH4)2SO40.3, CaCl2·2H2O 0.3, KCl 0.5, ZnSO40.3, MnSO40.23；Bi Chi
Yeast D13 culture mediums g/L：Mealy potato 200, glucose 20, with 10N H2SO4PH is adjusted to 2.5), being subsequently placed in 23 DEG C, 200rpm
Numerous culture is expanded in vibration in reciprocal shaker, until somatic cells quantity reaches 108A/mL.By Thiobacilus ferrooxidans LX5, oxygen
Change sulphur Thiobacillus TS6, Bordetella ZW2, Pichia pastoris D13 culture solutions by volume 1：5：2：2 mixing obtain composite microbial
Complex microorganism bacterium solution is seeded in anaerobically digested sludge system (bacterium solution volume 50mL, anaerobically digested sludge body by object bacterium solution
Product is 500mL), microbial nutrition microbial inoculum 15g is added, (the aeration quantity 6m under 23 DEG C, aeration condition3/(h·m2)) cultivated
Domestication, until sludge system pH, which is down to 3.0 stopping cultures, obtains first batch of acidification sludge.Then first batch of acidification sludge 500mL is taken again
Fresh 5000mL anaerobically digested sludges are seeded to, add microbial nutrition microbial inoculum the 10g, (aeration quantity under 25 DEG C, aeration condition
6m3/(h·m2)) culture domestication is carried out, until sludge system pH is down to 3.0 stopping cultures.It repeats the above process 3 times, gained acid
Change sludge as inoculum after domestication.
(2) bioleaching is handled
Pending anaerobically digested sludge is diluted to moisture content 98%, according to the volume ratio of inoculum and anaerobically digested sludge
1：5 by inoculum and anaerobically digested sludge be put into bioleaching reactor (be welded using 4mm steel plates, size for length
The long rectangular configuration of 1000mm × 800mm × 600mm, working volume 400L are separated into 2 corridors completely with steel plate along longitudinal direction
Road, each gallery set 2 baffle plates, interlock up and down, divide into 3 regions, and inside is equipped with aperture tube aerator.It is specific
Structure is see 200810242584.8 patent of Application No.).(the aeration quantity 6m under 23 DEG C, aeration condition3/(h·m2)) biology drip
Leaching for 24 hours, obtains the modified sludge of bioleaching, measures sludge system pH and be down to 2.0.
(3) filter-press dehydration
The direct press filtration of plate and frame filter press, feed time 60min, high pressure will be delivered to after bioleaching is nursed one's health by sludge pump
Hydraulic pressure squeezes pressurize 90min, feed pressure 0.6MPa, diaphragm pressing pressure 1.0MPa, and press filtration water colorless is clarified at this time, is squeezed out
Mud cake is in khaki, and odorless, moisture content of the cake is 58.3% after measured.
Embodiment 3
The anaerobically digested sludge bioleaching processing experiment of Zhengzhou sewage treatment plant
Mentioned technique according to the present invention, according to flow (see attached drawing 1) to sewage treatment plant's anaerobically digested sludge into
Row processing.About 500L factory's anaerobically digested sludges are taken, sludge fundamental property is as follows after measured：PH value is 7.32, solid content
3.6%, organic matter 48.6%, sludge specific resistance 6.37 × 1013M/kg, total nitrogen 33.1%, total phosphorus 25%.
(1) anaerobically digested sludge culture complex microbial community is utilized
Thiobacilus ferrooxidans LX5, Thiobacilus thiooxidans TS6, Bordetella ZW2, Pichia pastoris D13 are inoculated with respectively
(the Thiobacilus ferrooxidans LX5 culture medium g/L into respective culture medium：(NH4)2SO43.5, KCl 0.119, K2HPO40.58,
Ca(NO3)2·4H2O 0.168, MgSO4·7H2O 4.42, with 10N H2SO4 tune pH to 2.5；Thiobacilus thiooxidans TS6 culture
Base g/L：(NH4)2SO40.4, KH2PO43.0, MgSO4·7H2O 0.5, CaCl2·2H2O 0.25, sulphur powder 10；Bordetella
Bacterium ZW2 culture mediums g/L：(NH4)2SO40.3, CaCl2·2H2O 0.3, KCl 0.5, ZnSO40.3, MnSO40.23；Bi Chi
Yeast D13 culture mediums g/L：Mealy potato 200, glucose 20, with 10N H2SO4PH is adjusted to 2.5), being subsequently placed in 28 DEG C, 200rpm
Numerous culture is expanded in vibration in reciprocal shaker, until somatic cells quantity reaches 108A/mL.By Thiobacilus ferrooxidans LX5, oxygen
Change sulphur Thiobacillus TS6, Bordetella ZW2, Pichia pastoris D13 culture solutions by volume 3：2：1：1 mixing obtains composite microbial
Complex microorganism bacterium solution is seeded in anaerobically digested sludge system (bacterium solution volume 50mL, anaerobically digested sludge body by object bacterium solution
Product is 500mL), microbial nutrition microbial inoculum 25g is added, (the aeration quantity 5m under 28 DEG C, aeration condition3/(h·m2)) cultivated
Domestication, until sludge system pH, which is down to 3.0 stopping cultures, obtains first batch of acidification sludge.Then first batch of acidification sludge 500mL is taken again
Fresh 5000mL anaerobically digested sludges are seeded to, add microbial nutrition microbial inoculum the 15g, (aeration quantity under 28 DEG C, aeration condition
5m3/(h·m2)) culture domestication is carried out, until sludge system pH is down to 3.0 stopping cultures.It repeats the above process 5 times, gained acid
Change sludge as inoculum after domestication.
(2) bioleaching is handled
Pending anaerobically digested sludge is diluted to moisture content 98%, according to the volume ratio of inoculum and anaerobically digested sludge
1：8 are put into inoculum and anaerobically digested sludge to bioleaching reactor.(the aeration quantity 5m under 28 DEG C, aeration condition3/
(h·m2)) bioleaching 18h, the modified sludge of bioleaching is obtained, sludge system pH is measured and is down to 2.5.
(3) filter-press dehydration
The direct press filtration of plate and frame filter press, feed time 90min, high pressure will be delivered to after bioleaching is nursed one's health by sludge pump
Hydraulic pressure squeezes pressurize 120min, feed pressure 0.6MPa, diaphragm pressing pressure 1.0MPa, and press filtration water colorless is clarified at this time, is squeezed out
Mud cake in khaki, odorless, moisture content of the cake is 57.9% after measured.
Claims (10)
1. the bioleaching new method of a kind of anaerobically digested sludge, which is characterized in that comprise the following steps：
(1) complex microorganism bacterium solution culture
By the Thiobacilus ferrooxidans LX5 isolated from sludge, Thiobacilus thiooxidans TS6, Bordetella ZW2, complete red ferment
Female D13 is seeded to respectively in respective culture medium, be subsequently placed in 23~28 DEG C, in 180~200rpm reciprocal shakers vibration expand it is numerous
Culture, until somatic cells quantity reaches 108A/mL；Then by Thiobacilus ferrooxidans LX5, Thiobacilus thiooxidans TS6, rich
Moral spy Salmonella ZW2, Pichia pastoris D13 culture solutions are by volume (1~3)：(2~5)：(1~3)：(1~3) mixing obtains compound
Microbial inoculum；
(2) complex microorganism bacterium solution is tamed
Complex microorganism bacterium solution is seeded in anaerobically digested sludge system, adds microbial nutrition microbial inoculum, in 23~28 DEG C, exposure
Culture domestication is carried out under the conditions of gas, until sludge system pH, which is down to 3.0 stopping cultures, obtains first batch of acidification sludge；It then will be first batch of
Acidification sludge is inoculated to fresh anaerobically digested sludge, adds microbial nutrition microbial inoculum, under 23~28 DEG C, aeration condition into
Row culture domestication, until sludge system pH is down to 3.0 stopping cultures；It repeats the above process and complex microorganism bacterium solution is tamed and dociled
Change；
(3) bioleaching is nursed one's health
By the complex microorganism bacterium solution of domestication and anaerobically digested sludge by volume 1：(5~10) are mixed anti-to bioleaching
Answer in device, add in microbial nutrition microbial inoculum, 23~28 DEG C, aeration, bioleaching 12 under stirring condition~for 24 hours, obtain biology
Drip leaching sludge；
(4) filter-press dehydration
The direct press filtration under plate and frame filter press of sludge after bioleaching is nursed one's health carries out separation of solid and liquid, obtains moisture content and is less than
60% sludge cake.
A kind of 2. bioleaching new method of anaerobically digested sludge according to claim 1, which is characterized in that ferrous oxide
Thiobacillus LX5 culture mediums g/L：(NH4)2SO43.5, KCl 0.119, K2HPO40.58, Ca (NO3)2·4H2O 0.168,
MgSO4·7H2O 4.42, with 10N H2SO4Adjust pH to 2.5；Thiobacilus thiooxidans TS6 culture medium g/L：(NH4)2SO40.4,
KH2PO43.0, MgSO4·7H2O 0.5, CaCl2·2H2O 0.25, sulphur powder 10；Bordetella ZW2 culture mediums g/L：(NH4)2SO40.3, CaCl2·2H2O 0.3, KCl 0.5, ZnSO40.3, MnSO40.23；Pichia pastoris D13 culture mediums g/L：Potato
Powder 200, glucose 20, with 10N H2SO4Adjust pH to 2.5.
3. the bioleaching new method of a kind of anaerobically digested sludge according to claim 1, which is characterized in that described is micro-
Biological nutrition microbial inoculum is to use high nutrient as raw material, the solid matter obtained after microbial fermentation after filter-press dehydration, main
Nutrient is wanted to include N, P, K and Fe, Zn, Mn trace element.
4. the bioleaching new method of a kind of anaerobically digested sludge according to claim 1 or 3, which is characterized in that described
Microbial nutrition microbial inoculum add volume be anaerobically digested sludge 2~5%.
A kind of 5. bioleaching new method of anaerobically digested sludge according to claim 4, which is characterized in that step (2)
Middle complex microorganism bacterium solution is inoculated with by the 1~10% of anaerobically digested sludge volume；Step repeats the above process 3-5 in (2)
It is secondary that complex microorganism bacterium solution is tamed.
6. a kind of bioleaching new method of anaerobically digested sludge according to claim 1, it is characterised in that：The aeration item
Part refers to aeration control in 3~6m3/(h·m2)。
7. a kind of bioleaching new method of anaerobically digested sludge according to claim 1, it is characterised in that：The anaerobism disappears
Change moisture percentage in sewage sludge control more than 98%.
8. a kind of complex microorganism bacterium solution for handling anaerobically digested sludge, which is characterized in that by Thiobacilus ferrooxidans LX5, oxygen
Change sulphur Thiobacillus TS6, Bordetella ZW2, the compound compositions of Pichia pastoris D13.
A kind of 9. complex microorganism bacterium solution for handling anaerobically digested sludge according to claim 8, which is characterized in that oxidation
Ferrous Thiobacillus LX5, Thiobacilus thiooxidans TS6, Bordetella ZW2, Pichia pastoris D13 thalline quantity ratio for (1~
3)：(2~5)：(1~3)：(1~3).
10. applications of the Bordetella ZW2 (Bordetella sp.) in anaerobically digested sludge process field.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711449769.1A CN108083597B (en) | 2017-12-27 | 2017-12-27 | Composite microbial liquid for treating anaerobic digestion sludge and novel bioleaching method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711449769.1A CN108083597B (en) | 2017-12-27 | 2017-12-27 | Composite microbial liquid for treating anaerobic digestion sludge and novel bioleaching method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108083597A true CN108083597A (en) | 2018-05-29 |
| CN108083597B CN108083597B (en) | 2020-09-11 |
Family
ID=62180148
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711449769.1A Active CN108083597B (en) | 2017-12-27 | 2017-12-27 | Composite microbial liquid for treating anaerobic digestion sludge and novel bioleaching method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108083597B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109293216A (en) * | 2018-11-20 | 2019-02-01 | 湖南首创投资有限责任公司 | A kind of intelligent high-efficiency sludge dewatering system and technique |
| CN111517603A (en) * | 2020-05-06 | 2020-08-11 | 中国科学院成都生物研究所 | Biological reduction treatment technology for expanded sludge |
| CN111763648A (en) * | 2020-07-29 | 2020-10-13 | 中原工学院 | Acidophilic mixed strain and rapid preparation method and application thereof |
| CN112607988A (en) * | 2020-12-31 | 2021-04-06 | 广西壮族自治区环境保护科学研究院 | Biological regulator for reducing water content of sludge and application thereof |
| CN114380459A (en) * | 2021-12-15 | 2022-04-22 | 南京农业大学 | Method for treating farm wastewater through biological flocculation-electrodialysis and application thereof |
| CN114644999A (en) * | 2022-04-22 | 2022-06-21 | 南京农业大学 | Mycobacterium for efficient dehydration of anaerobic digestion sludge and application thereof |
| CN116817546A (en) * | 2023-08-28 | 2023-09-29 | 上海凌逍环保科技有限公司 | Dehydration treatment method, liquid fertilizer preparation method and organic fertilizer preparation method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103936246A (en) * | 2014-03-27 | 2014-07-23 | 南京农业大学 | New method for deeply dewatering sludge through bio-leaching process rapid conditioning |
| KR20160046718A (en) * | 2014-10-21 | 2016-04-29 | 주식회사 비엠 | Novel mixed strains BM-S-1A, Complex probiotics containning the same and Biological treatment method for purifying wastewater from leather industry using the same |
-
2017
- 2017-12-27 CN CN201711449769.1A patent/CN108083597B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103936246A (en) * | 2014-03-27 | 2014-07-23 | 南京农业大学 | New method for deeply dewatering sludge through bio-leaching process rapid conditioning |
| KR20160046718A (en) * | 2014-10-21 | 2016-04-29 | 주식회사 비엠 | Novel mixed strains BM-S-1A, Complex probiotics containning the same and Biological treatment method for purifying wastewater from leather industry using the same |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109293216A (en) * | 2018-11-20 | 2019-02-01 | 湖南首创投资有限责任公司 | A kind of intelligent high-efficiency sludge dewatering system and technique |
| CN109293216B (en) * | 2018-11-20 | 2019-11-19 | 湖南首创投资有限责任公司 | A kind of intelligent high-efficiency sludge dewatering system and technique |
| CN111517603A (en) * | 2020-05-06 | 2020-08-11 | 中国科学院成都生物研究所 | Biological reduction treatment technology for expanded sludge |
| CN111763648A (en) * | 2020-07-29 | 2020-10-13 | 中原工学院 | Acidophilic mixed strain and rapid preparation method and application thereof |
| CN112607988A (en) * | 2020-12-31 | 2021-04-06 | 广西壮族自治区环境保护科学研究院 | Biological regulator for reducing water content of sludge and application thereof |
| CN114380459A (en) * | 2021-12-15 | 2022-04-22 | 南京农业大学 | Method for treating farm wastewater through biological flocculation-electrodialysis and application thereof |
| CN114644999A (en) * | 2022-04-22 | 2022-06-21 | 南京农业大学 | Mycobacterium for efficient dehydration of anaerobic digestion sludge and application thereof |
| CN114644999B (en) * | 2022-04-22 | 2023-03-10 | 南京农业大学 | Mycobacterium for efficient dehydration of anaerobic digestion sludge and application thereof |
| CN116817546A (en) * | 2023-08-28 | 2023-09-29 | 上海凌逍环保科技有限公司 | Dehydration treatment method, liquid fertilizer preparation method and organic fertilizer preparation method |
| CN116817546B (en) * | 2023-08-28 | 2023-12-01 | 上海凌逍环保科技有限公司 | Dehydration treatment method, liquid fertilizer preparation method and organic fertilizer preparation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108083597B (en) | 2020-09-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108083597A (en) | A kind of processing anaerobically digested sludge complex microorganism bacterium solution and bioleaching new method | |
| CN103936246B (en) | A kind of sludge bioleaching method nurses one's health the method for deep dehydration fast | |
| CN102875210A (en) | Method for co-composting of blue-green algae and polluted bottom sediment | |
| CN107500497B (en) | Treatment method and system for black and odorous water body sludge | |
| CN108178341B (en) | Solid microbial purification particle and preparation method thereof | |
| CN105293845A (en) | Treatment method for sludge reduction | |
| CN110921850B (en) | Method for treating sewage by using microorganisms | |
| CN102874957B (en) | Method for processing liquid dung of pig farm through flocculation oxidizing resistance diminishing method | |
| CN103319218B (en) | Preparation method for composite microbial fertilizer with bioleached sludge and composite microbial fertilizer | |
| CN104817252B (en) | Method for improving dewatering performance of municipal sludge at low temperatures | |
| CN104338736A (en) | Perishable organic matter oxidizing agent, treatment system and method for preparing humic acid-contained fertilizers | |
| CN105238843B (en) | A kind of microbial flocculant, preparation method and applications | |
| CN104445851A (en) | Method for recycling sludge processing waste liquid to promote sludge harmless treatment | |
| CN105217911A (en) | A kind of bioleaching that utilizes reacts the technological process control carrying out sludge dewatering | |
| CN1314604C (en) | Highland and severe cold areas sewage treatment method | |
| CN102897923A (en) | Bioleaching method for promoting deep dehydration of water-blooming cyanobacteria | |
| CN105502852A (en) | Method for quickly treating refuse leachate of refuse incineration plant | |
| CN108128996B (en) | Composite microbial liquid for treating citric acid sludge and bioleaching treatment method | |
| CN1169945C (en) | Thiobacillus thiooxidans and biological eliminating method of chromium in tanning sludge | |
| CN103466791A (en) | Improved pre-acidifying treatment process of citric acid wastewater | |
| CN106316690A (en) | Method for produce liquid organic fertilizer with starch wastewater to improve saline and alkaline land | |
| CN103045649B (en) | Method for preparing microbial flocculant form potato starch wastewater as raw material | |
| CN108928911A (en) | A method of the degradation beneficiation wastewater COD based on sulphur flora | |
| CN207391228U (en) | Device for preparing organic fertilizer from sludge | |
| CN101723557A (en) | Micro-organism treatment process for printing and dyeing waste water |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |